FULANO ALEX MUREMI A22/0125/2003 BSC. AGRICULTURE (CROP PROTECTION) SUPERVISOR: PROF. MUTHOMI JAMES Dry bean leading legume consumed in Kenya Current yield status less than 500-1500kg Pests and diseases is a yield constraint Bean production majorly by small scale farmers Beans for food security Small scale farmers seldom use certified seeds Using quality seed is paramount seed purity germination capacity seedling infection Symptomatic Vs asymptomatic Planting disease free seeds (IPM) General objective To determine bean seed quality of samples obtained from farmers and markets in Bungoma East Sub-County Specific objectives To determination the proportion of pure seed component in the seed samples and proportion of other crop seeds, weed seeds and inert matter by weight in percentage To determine germination percentage and seed/seedling infections Sample Collection The bean samples were collected from individual farmers, Kimilili Town Market and Webuye Town Market at random Sample size 30 Sell 1 kg beans as seed? Determination of Purity Analysis Weighted 500 g of every sample Separated into fractions, bean seed, other crop seed, weed seed and inert matter and weighted Obtained normal looking bean seeds and malformed bean seeds from bean seed fraction and weighed Separated main bean variety and the other other bean varieties from normal looking seeds Determination of germination and seedling infection Observed aseptic condition 100 seed were blotted on moistened absorbent paper covered by another set and rolled accordingly Three replications for each sample placed in polythene sleeve and labeled Incubation Observation Moisture levels were checked Collected data on germination Physical purity The samples averagely had standards set for physical purity by KEPHIS Traces of other crop seeds for market samples Traces of weed seed sample in all the major sources Table of Bean Fraction, Other Crop Seed Fraction, Weed Seed Fraction and Inert Matter Fraction MAJOR SOURCES OF BEAN SAMPLES FRACTION % WEED OTHER SEED CROP SEED FRACTION FRACTION % % INERT MATTER FRACTION % KIMILILI TOWN MARKET 99.4 0 0 0.5 WEBUYE TOWN MARKET 99.5 0 0 0.5 FARMERS' OWNED 98.4 0.6 0 1.0 MEAN % 99.1 0.2 0 0.7 Varietal Purity Not varietally pure Samples having more than one variety Shrunken and discolored seeds Reduced quality Table of Normal Looking Bean Seed, Malformed Bean Seed, Main Bean Seed Variety and Other Bean Varieties MAJOR SOURCES OF SAMPLES NORMAL LOOKING BEAN SEED % MALFOR MED MAIN BEAN BEAN SEED OTHER BEAN SEED % VARIETY % VARIETIES % KIMILILI TOWN MARKET 89.9 8.9 68.8 30.7 WEBUYE TOWN MARKET 88.8 11.0 59.2 40.7 FARMERS OWNED 88.7 11.0 53.7 46.3 MEAN 89.1 10.3 60.6 39.2 Germination percentage Relatively fair germination Few vigorous seedlings Symptoms as diagnostic aspects of seedborne diseases Table of seedlings, dead seeds, moldy seeds, diseased seedlings and abnormal seedlings out of the total blotted seeds SOURCES OF COLLECTION % MOULDY ABNO. SEEDLI SEEDS/SE DISEASED BEAN BLOTTED NGGS DEAD EDLINGS SEEDLINGS SEEDLING SEEDS % SEEDS % % % S% KIMILILI TOWN MARKET 100 64 36 10 24 35 WEBUYE TOWN MARKET 100 58 43 7 19 33 FARMERS' OWNED MEAN 100 100 78 67 22 34 7 8 23 22 38 35 Seedlings Diseased seedlings Dead seeds and Moldiness Vigorous Seedlings Conclusion and Recommendations Conclusion The seeds were highly contaminated Recommendations There should be availability of clean planting material Holistic approach in ascertaining bean seed quality status Inclusion of a control
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