The Future of Foods Annual Report for the year A Centre of Research Excellence hosted by Massey University 2009 CONTENTS 03 04 06 Chairman’s review Directors’ report Highlights for 2009 08 12 17 Our strategy Our people Our science 27 28 Awards and achievements Statement of financial performance (CoRE activities) 29 30 Notes to the financial statements Scientific publications for 2009 Leading the crusade A New Zealand Centre of Research Excellence (CoRE) The Riddet Institute brings together leading scientists from Massey University (host institution), the University of Otago, The University of Auckland, AgResearch Limited and the New Zealand Institute for Plant and Food Research Limited. A partnership approach is central to our vision, with specialists pooling their expertise and resources and contributing crosssectoral perspectives to stimulate new ideas. Our CoRE is inclusive and unites key elements of New Zealand’s resources in food and related sciences. With discovery-based research and a high level of scholarship, the Riddet Institute is a catalyst for innovation to create sustained competitive advantage for New Zealand. Our contribution will be a legacy of world-class science that will act as a source of inspiration, particularly for young New Zealanders. Riddet Institute Centre of Research Excellence Annual Report 2009 1 2 Riddet Institute Centre of Research Excellence Annual Report 2009 chairman’s review Joining the forces Now, more than ever, New Zealand needs to carefully position and nurture its intellectual resources in our largest export sector – food. This sector is where New Zealand’s best comparative advantage lies, and it is our best hope for high returns on premium products in the future. W e are operating in a global economy where food supplies are dwindling and, paradoxically, where tranches of the world’s population suffer from obesity. There are many opportunities for clever science, but to make the best of them we need to work together across institutional boundaries and across disciplines and we need significant further financial commitment from the Government and from industry. At present, there is underinvestment in the critical opportunities for our nation. The Riddet Institute is unique in the food research sector – it is multi-disciplinary and brings together talent from the key science organisations concerned with food and health. This model is proving very successful and must be the way of the future for all New Zealand science. But the Institute cannot operate as a fledgling. To keep up in the world of international science it must have the resources to attract top researchers and scholars and be able to undertake a range of studies for industry to keep our companies competitive and at the forefront of innovation. New Zealand has top talent in this area of science, and it is vital to keep it. The eagle must be allowed to soar. Large companies in the New Zealand food industry recognise the need for investment in science. The Riddet Institute is backed by the larger food companies and we enjoy a productive and cordial relationship with them. Their support, both moral and financial, is unquestioned as we look to grow the Institute to give us the power to make a real difference. The third partner in this effort must be Government and its commitment to a prosperous economy based on natural advantage. This triumvirate represents a real hope for New Zealand. Finally, I would like to thank my fellow Board members and the Institute’s co-directors. We have had a busy year building strategic relationships and I have every confidence that now the Institute will move to the next level of effectiveness. We are poised and eager for that challenge. Dr James Watson Chairman Pre-eminence in agri-food research and scholarship is central to Massey University’s strategy. Our reputation for excellence in this area is greatly enhanced by having the Riddet Institute on our Manawatu campus. Steve Maharey Vice-Chancellor, Massey University Riddet Institute Centre of Research Excellence Annual Report 2009 3 directors’ report Meeting the challenge The Riddet Institute, a national Centre of Research Excellence (CoRE) devoted exclusively to the furtherance of the New Zealand food industry, plays a pivotal role in fortifying the New Zealand food industry with true scientific excellence. T hrough the CoRE research programme, we attempt to understand at a fundamental level the assembly of food structures, the changes in food structure that take place during the stages of human digestion and the consequent metabolic and physiological consequences. Food is central to New Zealand’s economy. More than half of our export income derives from food products, and our primary production exports are worth some $37 billion. New Zealand’s prime asset is its natural environment, including fertile soils and abundant rain and sunshine, all underpinning our primary production. As climate change, population growth and global demand for food, energy and water become limiting factors for world food production, New Zealand is going to have an important role to play in meeting world food needs. The future opportunities for New Zealand have never been brighter. More than ever before, our food industry relies on science and technology to ensure premium products, efficient processes and, increasingly, the development of very highvalue IP-embedded specialty food ingredients. Our Riddet Foodlink initiative, which you will read more about in this report, ensures that our research remains connected to those we are set up to serve. It is most gratifying to note that, over the last 12 months, those new scientific interconnections and synergies that we envisaged in establishing the Institute two years ago are now beginning to bear fruit. By way of example, the bioengineers from The University of Auckland led by Professor Andrew Pullan are now working closely with Distinguished Professor R Paul Singh, a Riddet Institute Principal Investigator from the University of California, Davis – one of the highest ranking food science institutions in the United States. He, in turn, is establishing close day-to-day connections with the gastrointestinal physiology teams at Palmerston North and Dunedin. What potential for breakthroughs in thinking! The old boundaries are coming down and a national critical mass in food and nutritional sciences is beginning to take shape. A specific indicator of general scientific quality and esteem is the recognition that the Riddet Institute has received from Wageningen University — arguably the most prestigious and well-founded university site for food science and nutrition research within Europe. Research leaders at Wageningen University have identified the Riddet Institute as a chosen partner for close collaboration. Recently, our Scientific Advisory Panel completed its first visit to the Institute. The panel includes Professor Eric Dickinson, University of Leeds, England, a food colloids expert; Professor Mark Wahlqvist, a human nutritionist with 4 Riddet Institute Centre of Research Excellence Annual Report 2009 a medical background; and Dr Peter Munro FRSNZ, General Manager, Ingredients Innovation, Fonterra Co-operative Group Ltd. The panel spent a very intensive week at the Institute, visiting most of our sites and groups. Its preliminary findings endorse strongly the Riddet Institute’s direction: “The panel is convinced of the importance of this ambitious research programme for the future delivery of better human health and wellbeing through the development of food innovation. The combination of experienced researchers and expertise assembled within this CoRE programme to address the issues of food structure change during human digestion is considered by the panel to be unique. An exciting feature of the Riddet Institute is the large number of excellent post-doctoral researchers and PhD students that have been recruited. This represents major capacity-building for New Zealand in terms of human capital and expertise.” During the year, there have been a number of highlights. In early 2009, the Institute held a political summit meeting and scientific conference, with the Rt Hon JB Bolger as our keynote speaker. Later in the year, we celebrated the launch of the Earle Food Research Fund, a $1 million fund aimed at recruiting and supporting star-quality PhD scholars. The name of the fund honours the many contributions to food technology by Massey University Professors Emeriti Richard and Mary Earle. The Earles attended and spoke at our successful two-day PhD colloquium event, an effective way to share knowledge across the Institute. The year saw much of our new laboratory equipment installed, giving us state-of-the-art facilities. In addition to the equipment purchased through the TEC Capital grant, we were most grateful to the Agilent Foundation for the generous donation to the Institute of specialist equipment and funding towards the QTOF mass spectrometer. Perhaps the major highlight for the year was the signing in England of a supply and distribution agreement between Speirs Nutritionals Ltd, a spin-out company of Riddet, and the multi-national Croda Europe Ltd. This will ensure that microencapsulation technologies invented by Riddet scientists will be marketed in all corners of the globe, with profits returning to New Zealand. The year 2009 has undoubtedly been another year of strong performance for the Riddet Institute. Our successes, however, are entirely due to the dedication of the scientists, support staff, Board members and wider stakeholders who collectively make up the Institute. To them, we say thank you. Special acknowledgement is due to the outstanding support given by the Vice-Chancellor of Massey University, our host organisation. Harjinder Singh PhD FRSNZ Paul J Moughan PhD DSc FRSNZ Professor Co-Director, Riddet Institute Distinguished Professor Co-Director, Riddet Institute Research leaders at Wageningen University sought a prestigious institution outside Europe to work with and after careful consideration, they identified the Riddet Institute as the chosen partner for close collaboration. Riddet Institute Centre of Research Excellence Annual Report 2009 5 Highlights for 2009 Scientific Advisory Panel appointed Earle Food Research Fund launched with $1 m for post-graduate scholarships Major agreement signed with Croda Europe and Speirs Nutritionals Ltd to market the Institute’s omega-3 emulsion technology Food conference and political summit held in Palmerston North Two-day PhD colloquium held for Riddet Institute post-graduate students Memorandum of Understanding signed with University of Shizuoka Purchase of QTOF mass spectrometer with generous assistance from the Agilent Foundation Partnership with Wageningen University formalised and will involve joint project on global food sustainability Publication of the textbook Advances in potato chemistry and technology edited by Riddet staff 6 Riddet Institute Centre of Research Excellence Annual Report 2009 10/09 02 /09 10/09 Prestigious scholarships Professor Harper honoured for his contribution to New Zealand science Student colloquium to be annual event The Earle Food Research Fund was launched in October. The million dollar scholarship fund has been named in honour of the contributions to the field made by Massey Professors Emeriti Richard and Mary Earle, who were international pioneers in food technology and engineering. Professor Mary Earle, Massey Vice-Chancellor Steve Maharey, Professor Richard Earle, Distinguished Professor Paul Moughan and Professor Harjinder Singh. At the Riddet Institute National Conference in February, Professor Jim Harper of the Ohio State University, USA, was presented with a greenstone adze in appreciation of his 28 years of research in New Zealand. Professor Harper worked full-time at the NZ Dairy Research Institute from 1981 to 1986 and has returned to New Zealand every year since for the three summer months. He has spent the last five summers at the Riddet Institute. Professor Harper is an internationally renowned food scientist and is an honorary Fellow of the Royal Society of New Zealand. In mid-October, our PhD scholars attended the Riddet Institute student colloquium in Palmerston North. As well as attending training sessions on IP management, written communication and presentation skills, each student gave a 15-minute presentation on their work, which was judged by three Riddet Institute senior scientists. Many senior scientists from around the country attended the presentations. During the colloquium, Professors Emeriti Richard and Mary Earle addressed the students and Professor Richard Earle gave a copy of their book Case Studies in Food Product Development to the student with the best presentation. PhD students Ranjita Sengupta (Massey University) (left) and Ofir Benjamin (University of Otago). Riddet Institute Centre of Research Excellence Annual Report 2009 7 Our strategy Science and targeted knowledge transfer The Institute’s goal is to be a premier centre for original research and scholarship, delivering measurable and sustainable benefits to all segments of the New Zealand food industry for the benefit of the nation. S trategies to realise the goal revolve around science and targeted knowledge transfer to underpin the development of innovative foods promoting health and wellness. Indicators of success include: • demonstrable and major benefits to individual sectors of the food industry, the industry as a whole and New Zealand’s economy • recognition by industry and Government that real benefits have been achieved and that the Riddet Institute has established its own clear and unique role. Strategic priorities • • • • World-class fundamental and strategic research Developing human capital (tomorrow’s leaders) Transferring new knowledge and technologies Partnering with industry to identify, capture and develop intellectual property • Exceptional international academic and industrial networks. Global connections In May, Distinguished Professor Paul Moughan, co-director of the Riddet Institute, helped broker a Memorandum of Understanding for co-operation in research and higher learning between Wageningen University in the Netherlands and Massey University.1 Wageningen University is the leading European university in agricultural and life sciences. The first project, named Proteos, will commence in mid-2010 and will involve Riddet Institute staff with expertise in protein science. JAPANESE CONNECTION Professor Harjinder Singh was appointed a Guest Professor at Kumamoto University’s Graduate School of Science and Technology in Japan. In November, he completed a series of lectures in food science at the university. He also initiated a joint project with Professor Shigeru Itoh, Shock wave and Condensed Matter Research Centre, on the shock wave processing of food materials. riddet fooDlink – demonstrating knowledge transfer Riddet Foodlink is a primary vehicle for the Institute to communicate with industry. Riddet Foodlink was set up to foster dialogue between industry and the Institute, to keep abreast of industry trends and issues and to ensure industry has access to the Institute’s scientific expertise. Forty-five companies belong to the network. Members receive preferential invitations to Riddet Institute conferences and events. The first meeting of members of the Riddet Foodlink network took place in late October 2009. Over a full day, members were updated on the latest research taking place at the Institute and on the broader R&D issues facing the New Zealand food industry. A further workshop is planned for the first half of 2010. other events At the beginning of the year, the Riddet Institute held a scientific conference over three days, attracting over 150 attendees. The speakers included leading international scientists from Canada, Japan, India, USA, UK and Australia. A further conference is planned in 2011. A Japan-New Zealand Functional Foods Workshop was held at the Institute at the end of July. Attended by 40 leading scientists including 12 Japanese experts, the two-day workshop was chaired by Professor Warren McNabb. Distinguished Professor Paul Moughan, Dr Aalt Dijkhuizen, Chairman of the Executive Board of Wageningen UR, (seated) and Professor Wouter Hendriks. 1 Following the agreement, the Wellington-based Ambassador of the Kingdom of The Netherlands, the late Ms Annelies Boogaerdt, visited the Institute in Palmerston North in July and again in August. Ms Boogaerdt died suddenly in Wellington in November, and the Institute co-directors express their great sadness at her passing. Ms Boogaerdt’s enthusiasm and her willingness to help forge closer links between the two institutions were most appreciated. 8 Riddet Institute Centre of Research Excellence Annual Report 2009 Strategy outcomes Case study Speirs goes for its best shot yet During the year, further development took place in the marketing of the Institute’s omega-3 microencapsulation technology through its joint venture company Speirs Nutritionals Ltd. A ‘nutritional shot’ has been developed that can deliver 100 percent RDI of EPA/DHA (500 mg) in a 6 ml sachet. T his shot can be consumed directly or mixed with yoghurt at the point of consumption. The product is stable for six months under refrigeration and does not have any perceivable off-flavour. This innovation is a significant improvement in the omega-3 product range, as all known competitive omega-3 products delivering over a 60 mg/serve have a perceivable fishy flavour. The ‘nutritional shot’ has been displayed in several exhibitions around the world through global marketing partner Croda Europe and has received huge interest from the food and nutraceutical industries. The product is now in the final stages of commercialisation. The Riddet Institute commercialised its patented technology for the microencapsulation of omega-3 fatty acids through its joint venture company Speirs Nutritionals Ltd in 2008. The company set up a state-of-the-art manufacturing facility in Marton, and the first commercial product was sent to Europe in 2009. Professor Harjinder Singh is the inventor of the encapsulation technology. One of the highlights of the year was the announcement in March 2009 that the Riddet Institute’s spin-out company Speirs Nutritionals Ltd would enter major world markets in partnership with Croda Europe Ltd. The exclusive supply agreement allows Croda to market omega-3 emulsions to food product manufacturers in the UK, Europe and North America. Speirs Nutritionals now microencapsulates Croda’s premium quality fish oil concentrates into omega-3 emulsions for incorporation in a wide range of solid and liquid food products. Croda is recognised globally as a leading industry manufacturer of innovative omega-3 products. Vice President for Croda Europe’s Health Care division, Dr David Cherry, said, “We chose Speirs as our exclusive supplier of omega-3 emulsions because we will gain competitive advantage by combining our high concentrated lipids with their New Zealand-developed microencapsulation technology. We consider the Speirs technology as a significant step forward in this market, allowing versatile delivery of very high omega-3 levels without any impact on taste. The outcome is Ωmelife™, a product that will lead the way on potency in the omega-3 food ingredients market.” General Manager of Speirs Nutritionals Ltd David Speirs said, “The Croda agreement confirms the value of the partnership we have formed with Riddet Institute in Speirs Nutritionals and provides us with a platform to work together on further innovations in the food sector.” The Croda agreement confirms the value of the partnership we have formed with Riddet Institute in Speirs Nutritionals and provides us with a platform to work together on further innovations in the food sector. Professor Gerald Tannock University of Otago Riddet Institute Centre of Research Excellence Annual Report 2009 9 Strategy outcomes collaboration Government policy input The Riddet Institute is one of six partners in Food Innovation New Zealand (FINZ), an initiative launched in August aimed at dramatically increasing New Zealand’s capacity in agri-food innovation and enabling the industry to better perform in domestic and international markets. FINZ partners are Massey University, AgResearch, Plant & Food Research, Fonterra, the Bio Commerce Centre and the Riddet Institute, with the support of Palmerston North City Council and Manawatu District Council. The FINZ partnership represents a globally significant collective of food innovation capability – more than 600 academic, research technical and commercial staff members and nearly as many under-graduate and post-graduate students. It is the largest collaboration of food innovation experts in the Southern Hemisphere. In March and June, the Institute welcomed a delegation of scientists from the University of Shizuoka in Japan. The Institute has built up a strong relationship with the university, and later in the year, a formal Memorandum of Understanding was signed to promote further joint activity. The Riddet Institute has key relationships with Government and provides input into New Zealand science policy. During 2009 Institute staff were involved in the following initiatives: • Professor Harjinder Singh was invited by the Ministry of Research, Science and Technology to be a member of a five-person exploratory mission to India. The delegation’s objectives were to better understand India’s true science capabilities and build relationships with key people in agriculture, biotechnology and food research. • In the first half of the year, the Institute hosted the Minister of Research, Science and Technology, the Hon Dr Wayne Mapp; the Hon David Carter, Minister of Agriculture; Nathan Guy, Associate Minister of Agriculture; the Hon Simon Power, MP for Rangitikei; senior officials from the Ministry of Agriculture and Forestry; opposition spokesperson for Research, Science and Technology, Ms Moana Mackey; and MP for Palmerston North Iain Lees-Galloway. • In February, the Institute held a half-day summit, the Future of Food, attended by over 150 eminent international scientists and key people in the food industry and in government. The Riddet Institute is one of six partners in Food Innovation New Zealand (FINZ), an initiative launched in August 2009. 10 Riddet Institute Centre of Research Excellence Annual Report 2009 Professor Harjinder Singh (second from left) was one of five delegates on a Ministry of Research, Science and Technology exploratory mission to India. • The Institute has hosted several visits from New Zealand Trade and Enterprise (NZTE) staff, who have been accompanied by representatives of overseas food companies. • Professor Harjinder Singh was invited by the Ministry of Agriculture & Forestry (MAF) to contribute to its strategic foresight project, the key focus of which was to explore the challenges and opportunities around achieving export-led economic growth. • Dr Mike Boland took part in the first EC-New Zealand Joint Science and Technology Cooperation Committee Meeting in Brussels in early June. • In August, Professor Harjinder Singh, Professor Warren McNabb, Dr David Everett and Dr Kyoung-Sik Han visited Korea to explore opportunities in functional food research. Professor Singh is the co-ordinator of the food innovation programme for the Korea-NZ Focal Point Programme. Outreach The Riddet Institute welcomes opportunities to talk to the general public, institutions and media about its science programmes. It is also part of the Association of Centres of Research Excellence (aCoRE) and participates in joint initiatives to increase awareness of the role played by the Centres of Research Excellence in the New Zealand economy. Individual staff also participate in wider programmes. For example: • Dr Sharon Henare was an invited participant of the Manu Ao Academy Working Party for assessment of measures of success for academic and professional development of Maori staff. She was also an invited participant of the Massey University working party for the Maori and Pasifika student success strategies planning workshop in October 2009. • Professor Harjinder Singh was invited by the Royal Society of New Zealand to give a speech to Members of Parliament at Parliament House in Wellington. The title of the address was ‘New perspectives on functional food development’. • Professor Paul Moughan was invited to address the Fonterra Shareholders’ Council in Auckland in May and the FAME course for young executives in Christchurch. • The Biotechnology Learning Hub, funded by MoRST to provide educational resources for teachers, completed two focus stories featuring the work of the Riddet Institute on omega-3 technology and on chemical analysis of the Maori potato (taewa). These stories involved extensive interviewing and filming of the scientists involved. The Rt Hon Jim Bolger attends an Institute half-day summit on the Future of Food. Jennifer Barclay, NZTE New York, met with Distinguished Professor Paul Moughan at the Riddet Institute in Palmerston North in the middle of the year. Riddet Institute Centre of Research Excellence Annual Report 2009 11 Our people Board of Directors Co-directors 1 2 3 4 5 6 7 8 9 Scientific Advisory Panel The Riddet Institute Scientific Advisory Panel was formed in May 2009. The panel’s role is to provide the Institute with advice on scientific, technical and research matters relating to research performance and science excellence. Members of the panel are Professor Eric Dickinson, Dr Peter Munro FRSNZ and Professor Mark Wahlqvist. The Scientific Advisory Panel conducts an annual research performance review by February each year. Professor Eric Dickinson completed a PhD at the University of Sheffield and was awarded a DSc by the University of Leeds. He has held academic appointments at the University of California, University of Oxford and University of Leeds. He has over 500 research publications and over 8,000 citations. He is a Fellow of several organisations including the Royal Society of Chemistry. He is currently Professor of Food Colloids, Procter Department of Food Science, at the University of Leeds, UK. 12 Riddet Institute Centre of Research Excellence Annual Report 2009 Dr Peter Munro gained his PhD at the University of London and is a Fellow of the Royal Society of New Zealand. He has lectured at the University of Auckland (Department of Chemical and Materials Engineering) and was Professor and Head of Department of Food Technology at Massey University, followed by roles at the New Zealand Dairy Institute and Fonterra Co-operative Group Ltd. He is currently General Manager, Ingredients Innovation, Fonterra Co-operative Group Ltd. Professor Mark Wahlqvist is a Fellow of the Royal Australasian College of Physicians and a Fellow and Life Member of the Nutrition Society of Australia. He holds several university appointments and is Director, Asia Pacific Health and Nutrition Centre, Monash University, Australia, and Visiting Professor at Hangzhou University of Commerce and Zhejiang University in China and at the National Health Research Institute, Taiwan He is immediate past-president of the International Union of Nutritional Sciences. 1. 2. 3. Dr James (Jim) Watson is well known in New Zealand scientific and business circles as the founder and CEO of Genesis Research and Development, a past-president of the Royal Society of New Zealand and past-chair of the National Science Panel. He has had a long career in health sciences and has held professorships at the University of California, Irvine, and the University of Auckland. Professor Robert Anderson is Pro ViceChancellor, College of Sciences at Massey University. He joined Massey University in 1971 and has a PhD from Cornell University, USA. In 2007, Professor Anderson was made an Officer, New Zealand Order of Merit. Dr Jeremy Hill is Group Director, Technology at Fonterra Co-operative Group Ltd. He graduated from Hull University in 1987 with a PhD, joined the staff of the New Zealand Dairy Institute (NZDRI) as a research scientist in 1991 and became Deputy Chief Executive of the NZDRI in 2000. He is a member of the National Committee of the International Dairy Federation (IDF) and President of the New Zealand IDF Committee. 4. 5. 6. Mr Peter Landon-Lane is CEO of Plant & Food Research. He was previously at the Fonterra Co-operative Group Ltd, where he held a number of senior positions in New Zealand, Japan, Taiwan and Europe. Prior to entering the dairy industry, he held Trade Commissioner roles in China and the Philippines. Dr Tracey McIntosh from the University of Auckland is a former co-director of Nga Pae o te Maramatanga, a sister Centre of Research Excellence. She has been a Fulbright Visiting Lecturer in New Zealand Studies at Georgetown, Washington DC, and has taught and researched in the Pacific, France and in central Africa. Professor Vernon Squire is Deputy Vice-Chancellor Academic and International at the University of Otago. He has a DSc from the University of Wales and graduated with a PhD from Cambridge where he was also employed subsequently for several years. He left the UK in 1987 to take up the post of Professor of Applied Mathematics at the University of Otago. 7. 8. 9. Dr Andrew West has been Chief Executive of AgResearch since May 2004. In the late 1980s and early 1990s, Dr West played a major role in the New Zealand Government’s science reforms. In the late 1990s, he was Chief Executive of the New Zealand Qualifications Authority, and from 2001 to 2004 he was Executive Chairman of the Tertiary Education Commission. Distinguished Professor Paul Moughan graduated PhD from Massey University in 1984 and was awarded Doctor of Science in 1996. He received the Distinguished Professor Award from Massey University in 2005 following international peer review. He has published more than 300 scientific works and is a Fellow of the Royal Society of New Zealand. Professor Moughan was co-founder of the Riddet Institute in 2003 and prior to that was Head of the Institute of Food, Nutrition and Human Health at Massey University. Professor Harjinder Singh is co-founder of the Riddet Institute. He graduated PhD from the National University of Ireland, University College, Cork, in 1986. He is a Fellow of the Royal Society of New Zealand and Fellow of the International Academy of Food Science and Technology. He is ranked in the world’s top 15 most highly cited agricultural scientists and was presented with the William Haines Dairy Science Award by the California Dairy Science Foundation (USA) and the Massey University Research Medal in 2008. Administration Group from left: Paula McCool (Communications Officer), Dr Mike Boland (Principal Scientist and Executive Officer), John HenleyKing (Project Manager), Professor Harjinder Singh (Co-director), Felicia Stibbards (Business Administrator), Terri Palmer (PA to the Directors), Willi Twight (Operations Manager), Distinguished Professor Paul Moughan (Co-director). Riddet Institute Centre of Research Excellence Annual Report 2009 13 Principal Investigators Advancing the opportunities 1 2 3 5 6 7 9 14 Riddet Institute Centre of Research Excellence Annual Report 2009 4 8 1. 2. 3. Professor Richard Archer holds a PhD from Massey University, where he is currently Professor and Head of the Institute of Food, Nutrition and Human Health. He is a Fellow of the Institution of Professional Engineers of New Zealand and former Head of the Institute of Technology and Engineering at Massey University. Professor Archer’s career has focused on process engineering research and its commercialisation. Professor Geoffrey Jameson graduated with a PhD from the University of Canterbury in 1971. He then spent nearly 20 years overseas in Switzerland and at Georgetown University in Washington DC before joining Massey University in 1994. Professor Jameson is a Fellow of the Royal Society of New Zealand and Director of the Centre for Structural Biology at Massey University. Dr Nigel Larsen graduated from Massey University in 1980 with a PhD in Chemistry. He is Science Group Leader, Bioresources Engineering & Chemistry, Plant & Food Research, Lincoln, Canterbury. He is widely recognised as an authority on the processing of foods and grains and is a specialist in bakery products. 4. 5. 6. Professor Warren McNabb has a PhD from Massey University, where he is an Adjunct Professor. In 2009, he was appointed General Manager, Food & Textiles Group at AgResearch. Professor McNabb is a Fellow of the New Zealand Institute of Agricultural and Horticultural Sciences. Professor Jim Mann is a physician and Professor of the Department of Human Nutrition and of the Department of Medicine at the University of Otago. He is Director of the Edgar National Centre for Diabetes Research, Co-director of the WHO Collaborating Centre for Human Nutrition and a Fellow of the Royal Society of New Zealand. Professor Mann is a Companion of the New Zealand Order of Merit. He was educated at the University of Cape Town and the University of Oxford. Professor Laurence Melton graduated with a PhD in Organic Chemistry from Simon Fraser University in Canada in 1974. He is Director of the Food Science programmes at the University of Auckland and Professor of Food Chemistry. He is a Fellow of the Royal Society of Chemistry, Fellow of the American Institute of Chemists and Fellow of the International Academy of Food Science and Technology. 7. 8. 9. Professor Andrew Pullan is head of the Department of Engineering Science at the University of Auckland. He graduated from the University of Auckland with a PhD in Engineering in 1988. He leads a research team of seven post-doctoral fellows and six PhD and master’s students. Professor Pullan was elected as a Fellow of the Royal Society of New Zealand in 2009. Distinguished Professor R Paul Singh graduated PhD in Agricultural Engineering from Michigan State University in 1974. He was inducted into the US Food Engineering Hall of Fame in 2003. He is Distinguished Professor of Food Engineering at the University of California, Davis, and a member of the US National Academy of Engineering. Professor Gerald Tannock joined the University of Otago in 1974 and was awarded a Professorial Chair in 1996. He held a half-time position in the Department of Agricultural, Food and Nutritional Science at the University of Alberta, Edmonton, Canada, from 2001 to 2005. He was awarded a Royal Society of New Zealand Silver Medal in 2000 for his contributions to science and technology and was elected a Fellow of the American Academy of Microbiology in 2002. Associate Investigators Dr Eric Altermann Dr Mike Boland Associate Professor John Bronlund Dr Lawrence Creamer, FRSNZ Professor Clive Davies, FRSNZ Dr Kevin Davies Professor Peter Derrick Dr David Everett Professor Juliet Gerrard Associate Professor Matt Golding Associate Professor Paul Kilmartin Dr Julian Lee Professor Roger Lentle Dr Duncan McGillivray AgResearch Massey University Massey University Massey University Massey University Plant & Food Research Massey University University of Otago University of Canterbury Massey University The University of Auckland Plant & Food Research (retired 2009) Massey University The University of Auckland Associate Professor Kate McGrath Professor Robert McLachlan, FRSNZ Dr John Monro Dr Gill Norris Professor Charmian O’Connor, FRSNZ Professor David Parry, FRSNZ Dr Nicole Roy Dr Tanya Soboleva Dr Juliet Sutherland Professor Graeme Wake, FRSNZ Dr Bill Williams Professor Ray Winger Victoria University of Wellington Massey University Plant & Food Research Massey University The University of Auckland (retired 2009) Massey University AgResearch AgResearch Plant & Food Research Massey University Massey University Massey University Riddet Institute Centre of Research Excellence Annual Report 2009 15 Research and technical staff Post-graduate students Dr Payel Bagga Dr Libei Bateman Valentine Borges Dr Guillaume Brisson Steven Chalmers Jack Cui Dr Shantanu Das Dr John Davidson Binosha Fernando Dr Maria Ferrua Janiene Gilliland Dr Derek Haisman Chris Hall Dr Kyoung-Sik Han Dr Sharon Henare Dr Jason Hindmarsh Dr Lovedeep Kaur Dr Blair Lawley Dr Simon Loveday Dr Carlos Montoya Russell Richardson Shane Rutherfurd Nok Sawatdeenaruenat Dr Jaspreet Singh Dr Jiahong Su Amit Taneja Namrata Taneja Charlie Towler Dr Aiqian Ye Dr Peter Zhu Maggie Zou Visaka Anantawat Prabhu Balan Ofir Benjamin Lakshmi Chaitanya Selina Chan Sylvia Chung Leah Coles Anant Dave Eli Gray-Stuart Shiromani Jayasekara Sandra Kim Moritz Lassé Megan Levers Davide Mercadante Warren Miner-Williams Arup Nag Mallesh Rao Peram Daniel Ries Emmanuelle Riou Anwesha Sarkar Horng Yuan Saw Ranjita Sengupta Christina Streicher Jiahong Su Richard Sun Amit Taneja Lisa Te Morenga Dulantha Ulluwishewa Amy Van Wey Xiangli Wang Teresa Wegrzyn Hongyan Yao Research Officer Research Associate Technician Post-doctoral Fellow IT and Web Consultant Technologist Product Development Manager Post-doctoral Fellow Technician Research Officer Technician/Laboratory Supervisor Senior Technical Advisor Food Technologist Post-doctoral Fellow Post-doctoral Fellow Research Officer Post-doctoral Fellow Post-doctoral Fellow Research Officer Post-doctoral Fellow Technical Officer Senior Research Officer Technician Research Officer Research Assistant Research Officer Technician Researcher Senior Research Officer Technologist Research Associate Resident visiting scientists Professor Douglas Dalgleish Dr Diego Genovese Professor Jim Harper Professor Andy Rao Dr Lindsay Sawyer 16 University of Guelph, Canada PLAPIQUI (UNS-CONICET), Argentina Ohio State University, USA Cornell University, USA University of Edinburgh, UK Riddet Institute Centre of Research Excellence Annual Report 2009 Massey University Massey University University of Otago Massey University The University of Auckland Massey University Massey University Massey University Massey University Massey University Massey University University of Canterbury University of Otago The University of Auckland Massey University Massey University Massey University Massey University Massey University Massey University Massey University Massey University Massey University Massey University Massey University Massey University University of Otago Massey University Massey University Massey University Massey University Massey University PhD PhD PhD PhD PhD PhD PhD PhD PhD MSc PhD PhD MSc PhD PhD MSc PhD PhD PhD PhD PhD PhD MSc PhD PhD PhD PhD PhD PhD MSc PhD PhD ENSAIA, Nancy-Université ENSAIA, Nancy-Université University of Hohenheim Technical University of Berlin University of Copenhagen University of Copenhagen Technical University of Berlin Amity Institute of Biotechnology Amity Institute of Biotechnology Indian Institute of Technology University of the South Pacific AgroParisTech University of Vienna Singapore Polytechnic France France Germany Interns 2009 Anne Dartois Michael Foucault Katja Mader Daniela Endt Kristina Bak Japelt Carina Svendsen Thilo Berg Devesh Chandra Pant Darshan Kumar Monish Salhotra Sainimili Vaubula Mateyawa Aymeric Autheman Natascha Stroebinger Zhao Zhuo Germany Denmark Denmark Germany India India India Fiji France Austria Singapore Our science We are at the nexus of food science and nutrition Our goal is to generate essential underpinning knowledge that will provide the base of tomorrow’s innovations in advanced foods. Our research focuses on the nexus of food science and human nutrition and falls into three broad areas: Future Foods – Inspired by Nature (CoRE research programme) Functional Foods and Ingredients Personalised Foods Fundamental underpinning science and unique national competency Functional Foods and Ingredients Future Foods Inspired by Nature Personalised Foods industry-focused research and development Riddet Institute Centre of Research Excellence Annual Report 2009 17 Our science Future Foods – Inspired by Nature ‘Natural structures’ in foods not only provide textures and flavours and supply nutrients, but also support metabolic regulation of energy and the controlled absorption of nutrients and bioactive compounds. O ur hypothesis is that food behaviour post-ingestion, and the consequent availability of nutrients, can be managed through selecting and manufacturing of food ingredients to produce food structures that will control the release of nutrients and bioactive molecules. These reengineered structures will mimic the functions of the ‘natural’ structures and will change in response to external stimuli in a predictable manner, for example, they may be digested rapidly enough to be an efficient delivery carrier of nutrients and bioactive molecules, but slowly enough to preserve the structure during the tasting and perception processes. Our work in this area is divided into four platforms: Food Materials and Structures Gastrointestinal Biology Modelling and Engineering Innovative Food Solutions innovative food solutions Food Materials and Structures Gastrointestinal Biology Modelling and Engineering Chemistry • Physics • Mathematics • Engineering • Biology • Materials Science Nanotechnology • Digestive Physiology • Nutrition • Microbial Ecology 18 Riddet Institute Centre of Research Excellence Annual Report 2009 Our science Future Foods – Inspired by Nature > Food Materials and Structures We want to determine underlying fundamental principles controlling the assembly, breakdown and behaviour of food structures and to understand how these processes work. Project examples: • Interfacial engineering and properties of food emulsions (Project leader: Professor Harjinder Singh) • Ingredient interactions in complex foods (Project leader: Professor Laurence Melton) • Protein self-assembly and nanostructures in foods (Project leader: Professor Geoffrey Jameson). Profile > Protein nanostructuring and nanofibril formation The texture of food and bioavailability of nutrients all stem from the molecules and ions that comprise the food, from the complex interactions amongst these components – the nano- and microstructures that result from these interactions – and from the changes in interactions and structuring that take place on various treatments, such as heat, pressure and pH . O ne nanostructuring of particular interest involves the formation of nanofibrils (also called beta-fibrils) from food proteins (Figure 1). These structures, once formed, can be very stable and resistant to breakdown by heat, acid and digestion by proteases. Riddet Institute Investigators Simon Loveday and PhD student Anant Dave (Massey University), Juliet Gerrard (University of Canterbury), Nigel Larsen (Plant & Food Research) and PhD student Moritz Lassé (University of Canterbury) are investigating the extent to which food processing conditions may favour formation of nanofibrils from key food components and how this might impact on food safety, texture and nutritional quality. The model proteins employed are ß-lactoglobulin from milk (Figure 2a) and ovalbumin (Figure 2b) from hen egg whites. What happens to the formation and stability of these fibrils when additional components used in food preparation are added, such as whole eggs, whole milk, flour and sugar? Do these nanofibrils have any health consequences? How important are these fibrils to food texture? These key questions are under investigation. Proteins have remarkably flexible structures – Figure 2 gives a misleading picture of a rigid protein structure – a picosecond (10-12) freeze-frame snapshot of a molecule that is, in fact, highly dynamic. Groups of atoms can move back and forth over distances of up to 0.5 nm on time scales ranging from picoseconds to milliseconds for slow ‘breathing’ motions. It is the structure and, crucially, these internal motions that determine how proteins and other large molecules such as polysaccharides interact. These interactions are the basis of food textures, nutritional availability and behaviour during food processing. Therefore, in order to rigorously test ideas on how proteins determine food structures and properties, we must be able to prepare ‘designer’ proteins and enzymes to study in the lab. However, many proteins, especially milk proteins such as bovine ß-lactoglobulin, when synthesised outside their natural host, behave poorly, often because of the sulphur-containing amino acid cysteine (these cysteines are highlighted in Figure 2). After more than five personyears of effort by technician Trevor Loo, post-doctoral fellow Komala Ponniah and master’s student Kristy Baker, supervised by Riddet Institute Investigators Gill Norris and Geoff Jameson, we have finally cracked this problem and can prepare fully functional synthetic bovine ß-lactoglobulin using a special Escherichia coli expression system. Potentially, other milk proteins can be similarly prepared. Importantly, with this expression system, we can make 13C and 15N isotopically labelled protein. With isotopically labelled protein, advanced nuclear magnetic resonance (NMR), a technique related to MRI, can then be used to study these internal protein motions and inter-molecular interactions (Figure 3). Very recently, master’s student Kristy Baker, working with Pat Edwards, technical director of the BioNMR facility, discovered by NMR methods a key hitherto undiscovered breathing motion of bovine ß-lactoglobulin, hinged at the junction of two sub-domains (see Figures 2a and 3b; Tyr20 sits at this hinge point). This motion may be the prelude to denaturation of bovine ß-lactoglobulin, which can lead to the nanofibrils described in the previous paragraph. As part of Platform 1, techniques are being developed to study more closely the assemblies and interactions of molecules. In addition to NMR methods using Massey University’s 700-MHz NMR spectrometer with cryoprobe, especially utilising a unique high-pressure/high-temperature cell, advanced methods for studying nano-sized molecular complexes with huge masses are being developed around the 9.4 Ts FT-ICR mass spectrometer, an instrument with the highest field in Australasia (Figure 4). Riddet Institute Centre of Research Excellence Annual Report 2009 19 Our science Future Foods – Inspired by Nature > Food Materials and Structures > Profile (a) (b) fig.1 Nanofibrils Many globular proteins, especially on heating at low pH, are susceptible to losing helical structural elements and to refolding into a polymeric form, characterised by a continuous ß-sheet. There is evidence that these structures may form in processed foods, and we are assessing how these structures might impact on food quality and safety. Fig. 1(a) A model for a ß-sheet nanofibril. Fig. 1(b) Electron micrograph of nanofibrils formed on heat denaturation of bovine ß-lactoglobulin at low pH. 500 nm fig.2 Protein structures The structures of two key proteins of food ingredients shown in cartoon representation. Protein structures are generally comprised of three main motifs, usually referred to as secondary structure: (1) the arrows represent beta strands, where the protein adopts a stretched out conformation and adjacent strands are linked by hydrogen bonds of the main chain, (2) the helices represent alpha helices, where the protein is coiled up and hydrogen bonds link coils of the helix and (3) loops or random coil, which link beta strands into sheet-like structures to alpha helices. Fig. 2(a) The structure of bovine ß-lactoglobulin. This protein, despite much study by a myriad of techniques, retains many mysteries, especially why it is present in some milks and not in others (e.g. human milk) and what is its physiological function in milk. Its denaturation properties are important in many foods. New Zealand cows express one, other or a binary mixture selected from among three natural variants – A, B and C. Key sulphur-sulphur bridges and the sites of difference among naturally occurring variants are highlighted. Fig. 2(b) The structure of egg-white ovalbumin. The mechanical denaturation of this protein is the basis of pavlovas. However, little is known of its predilection for formation, especially in the presence of other food components, and of the consequences of these nanostructures in food. (a) (b) A/B substitution site: Asp64Gly EF loop ‘closed’ at low pH Val118Ala Disulfide bridges Dimer interface Tyr20 B/C substitution site: Gln59His 20 Riddet Institute Centre of Research Excellence Annual Report 2009 Our science Future Foods – Inspired by Nature > Food Materials and Structures > Profile fig.3 Protein structures Fig. 3(a) Two-dimensional NMR spectrum of bovine ß-lactoglobulin. For this 15N-labelled protein, each peptide NH moiety has a distinct peak in this contour map. The three natural variants – A, B and C – of bovine ß-lactoglobulin are superimposed to show both similarities and subtle differences in structure. Together with this and other NMR techniques, the internal motions of this protein can be deciphered. Fig. 3(b) The unusually long time scale for motions of a key tyrosine residue for variant A of ß-lactoglobulin is shown. This tyrosine, Tyr20, which sits at the intersection of the two ß sheets that comprise the barrel of ß-lactoglobulin is highlighted in Fig. 2(a). from left: Dr Gillian Norris, Kristy Baker, Professor Geoffrey Jameson and Dr Pat Edwards. (a) fig.4 Mass spectrometer The partly assembled 9.4 Ts FT-ICR mass spectrometer. This researchgrade instrument, designed by Riddet Investigator Peter Derrick (Massey University), will provide leadingedge capabilities for characterising large weakly associated molecular complexes. Key technical staff of the Institute of Fundamental Sciences, Massey University (left to right: Igor Filipov, Steve Denby and Dave Lloyd) are installing and testing the detector. The high-field liquid-helium cooled superconducting magnet stands behind them. Figures are courtesy of Patrick Edwards (Technical Director of the Massey University BioNMR Facility) and Kristy Baker (MSc student). (b) Tyr20 Riddet Institute Centre of Research Excellence Annual Report 2009 21 Our science Future Foods – Inspired by Nature > Gastrointestinal Biology The human gastrointestinal tract (GIT) is a fitting target for the development of specialised functional foods, acting as it does on the interface between diet and the metabolic events that sustain life. We want to understand how the GIT functions and how it processes different diets and food materials. Project examples: • Foods targeting nutrient uptake kinetics, satiety and gut function (Project leader: Professor Paul Moughan) • The genetic and physiological basis for biotransformations of food (Project leader: Professor Warren McNabb) • Dietary intervention targeting physiological endpoints (Project leader: Professor Jim Mann). Profile > Feeding our gut not so simple – there is always a barrier The human intestine, with a surface area of 400 m2, is the largest interface between humans and their environment. The intestinal barrier consists of a complex array of cell types and acts as a ‘biological bouncer’ to protect the body from the entry of microbes and antigens. W hile, in healthy individuals, this barrier provides protection from pathogenic microbes and promotes the uptake of nutrients, a disruption of the fragile balance erodes our wellbeing. Our ‘gut’ is really at the centre of how ‘good’ we feel. Ultimately, this balance is maintained by complex interactions of genetic networks both within and between the host and their microbial community whereby marker molecules can trigger individual responses and cascades. An internationally recognised research team headed by Warren McNabb and Paul Moughan is investigating these interactions between intestinal cells, nutrients and microbes promoting health. Two of the projects, which are led by Nicole Roy and Eric Altermann from AgResearch, are focused on the human intestinal barrier function. The barrier can be divided into four components: a physical barrier, a chemical barrier, an immunological barrier and a microbial barrier (with some trillions of resident bacteria). Intestinal bacteria are involved in a molecular ‘cross-talk’ with the other intestinal barrier components and play functional roles in the development of the intestine and the maturation of the intestinal immune system. Dulantha Ulluwishewa (supervised by Warren McNabb, Rachel Anderson, Nicole Roy, Jerry Wells and Paul Moughan) is studying the physical barrier, which is a single layer of epithelial cells (epithelium) with tight junctions between adjacent cells. The tight junctions are multi-protein complexes that affiliate together and act like glue, and they play a critical role in determining the permeability, function and health of the intestine. Dulantha aims to understand the ‘cross-talk’ between epithelial and dendritic cells in response to intestinal bacteria. Dulantha is developing a unique model of the epithelium where the luminal aspect is exposed to an anaerobic environment and thus is more representative of 22 Riddet Institute Centre of Research Excellence Annual Report 2009 the actual intestine. In 2009, Dulantha spent six weeks in the laboratory of Professor Jerry Wells, Head of Host-Microbe Interactomics at Wageningen University. Dulantha learnt techniques for investigating how microbes can alter dendritic cell function. Ranjita Sengupta (supervised by Warren McNabb, Eric Altermann, Nicole Roy and Paul Moughan) is comparing two strains of Lactobacillus fermentum that have diametrically opposed effects on intestinal barrier function to study the molecular basis of these differences. Her work aims to identify important genetic differences in these strains, which will provide a better understanding of the effect that individual bacteria can have on intestinal barrier function. The long-term goal is to understand how foods interact with our resident microbes and epithelial and immune cells to maintain intestinal barrier function and to use the resulting knowledge and IP to develop new ingredients that can add value to ‘smart foods’ because they promote our health and wellness. Dulantha Ulluwishewa. Our science Future Foods – Inspired by Nature > Modelling and Engineering We are developing advanced mathematical and computational models of aspects of the human gut and subsequent food processing systems, together with the experimental techniques and instrumentation required to validate the models. This platform underpins all the other platforms and, importantly, contributes to the development of innovative food formulations and creative process specifications for food manufacturing. Project examples: • Modelling of the human digestive process (Project leaders: Professors Andrew Pullan and R Paul Singh). Profile > Understanding the way we chew could contribute to formulation of new foods Mastication, or chewing, is a complex process in which food is crushed and ground by teeth resulting in increases in the surface area of foods to allow more efficient breakdown by enzymes. A fter chewing, the food (now called a bolus) is swallowed, and it enters the oesophagus and continues on to the stomach, where the next step of digestion occurs. Work has begun on understanding the processes of mastication and bolus formation to predict the changes of food structure over the course of chewing. New insights will be found into how we chew and how foods could be formulated to exploit this behaviour for sensory or nutritional benefit. Not only engineering and modelling aspects are under study, but also human physiology and behaviour. To this end, collaborations have been developed with researchers nationally and internationally. Mastication decreases particle size of foods (increases surface area) by fracture for brittle foods, or cutting and shearing for more fibrous materials. Saliva addition causes liquid bridging between particles, providing cohesion and lubrication of the bolus. Some of the added moisture is absorbed into the food particles allowing solutes to be leached out. The fact that these solutes are tasted shows that some of the moisture added is subsequently lost from the bolus. It is commonly reported that as little as 40 percent of the initial food weight is present in the bolus at the point of swallow, demonstrating that particles as well as solutes are lost into the oral cavity during chewing. All these factors show that there are many mechanisms and interactions to deal with both in mathematical modelling and experimentation. Mathematical modelling work is being undertaken by a PhD student (Eli Gray-Stuart) under the supervision of Massey University’s Jim Jones, John Bronlund, Robert McLachlan and Jason Hindmarsh. Through the application of mathematical modelling, the PhD project will provide tools and techniques that can be used to extract further understanding of human mastication from the experimental data collected in other projects. Over the last decade, Peter Xu and John Bronlund have been conducting research on building and controlling masticatory robots. A full 3D parallel robot jaw has been designed following human biomechanical principles and is capable of accurately reproducing recorded human jaw movements during chewing of foods. Richard Sun and the chewing robot. This work has been carried out by post-graduate students J-Sebastian Pap and Jonathan Torrance and was supported by a Foundation for Research, Science and Technology project led by Andrew Pullan. To replicate chewing trajectories of the molar teeth during chewing, a more simple 2D linkage robot was built by post-graduate students Darren Lewis and Richard Sun in collaboration with Plant & Food Research. With support from the Riddet Institute, the functionality of the robot has been extended to include food manipulation between chewing cycles and enhanced control. Each of these robots can be used to mimic human chewing and prepare boluses for in vitro digestion assays. Information on the magnitude and direction of forces applied to the food can be collected in real time, and the resulting bolus can be assessed. Modelling of these phenomena requires good-quality data on the breakage properties of foods and how they are masticated in humans. A significant research activity has been established that focuses on the chewing behaviour of humans and how this is influenced by food properties. This includes investigation of the changes in particle size distribution and bolus composition for a range of different food systems. Riddet Institute Centre of Research Excellence Annual Report 2009 23 Our science Future Foods – Inspired by Nature > Innovative Food Solutions We want to create completely new processes or processing routes for the development of new products, in particular, those that could deliver personalised high-quality food products. Novel food concepts, processes and services are being developed based on the knowledge generated by all the research platforms and existing knowledge in the scientific literature. Project examples: • Technofoods (Project leader: Professor Richard Archer) • Novel technologies for food extrusion (Project leader: Dr Nigel Larsen) • Future market directions (Project leader: Professor Ray Winger). Profile > Fast food of the future? Professor Richard Archer and Associate Professor Matt Golding of Massey University in Palmerston North are imagining the fast food of the near future. T hey imagine the sms-Twitter generation reacting against the slow-organic-real-local food trends of their parents. They are building a system for making foods at point of sale by three-dimensional printing. Such printing is the basis of rapid prototyping where a thread of molten plastic is laid down layer by layer to build up an object of any shape. In the food case, a food paste is laid down drop by drop, coloured and flavoured as it happens. A coloured, flavoured 3D shape is built up rapidly then cooked fast. This is not a food we know. It has no name. There are no rules. It only exists as a software file before becoming real. You can download it or create it yourself or toss digital coins to determine what you get. Secretly, such food can be highly nutritious and carry many healthy features – but don’t tell the kids that. PhD student Teresa Wegrzyn is studying the issue of how to create a controlled solid food foam structure during the rapid and somewhat heterogeneous process of microwave cooking. She is using very simple starch plus polysaccharide batters (potentially gluten-free) as the initial model to understand the fundamentals. Ultimately, 3D printed foods must use batters that will be stable for months as a paste, then flow during printing, then set up instantly once deposited and hold shape during cooking as well as provide a nucleation template for the final food structure. Fellow PhD student Sandra Kim is studying colouration of constructed food. Ideally, she can take a .pix file from a mobile ‘phone camera and calculate for each volume element (voxel) of batter the quantity of food dye to use from those available in the printer cassette. The colour must come out just right after cooking, so the calculation model 24 Riddet Institute Centre of Research Excellence Annual Report 2009 must account for spectral blending, dispersion and diffusion, partitioning between food matrix elements, changes in matrix colour background during cooking, reflectance effects of the surface and reactions of the dye itself. A significant milestone this year was the development of the first printer prototype based on a Cartesian robot. Final year 2009 engineering students Grant Ramsay and Jacob Pemberton, supervised by Gourab Sen Gupta of Massey and Terry Southern of Beta Solutions Ltd, have developed the first printer prototype based on a Cartesian robot. They have developed first cut printer driver files and image processing routines to pump batter and flavour and colour and mix it all in line. A new pair of mechatronics students will continue developments in 2010. From left: PhD student Teresa Wegrzyn, Professor Richard Archer, Associate Professor Matt Golding, Dr Gourab Sen Gupta, and PhD student Sandra Kim. Our science Functional Foods and Ingredients Knowledge of foods and their formulations as they pertain to human health and wellness will allow industry to develop new functional foods and novel ingredients. To provide this knowledge, we have to understand the structures and functions of food materials and their functionality in terms of health effects, sensory attributes and other quality attributes. The Riddet Institute is undertaking a range of projects to develop new functional foods for its industry clients. Profile > Food synergy – an example with kiwifruit Food science has traditionally been carried out using a reductionist approach, studying a single food type. This has been partly because a simpler system is easier to study and interpret, but has also been encouraged by the sector-based nature of R&D funding and the expected commercial benefits of R&D. I n New Zealand, there has traditionally been a separation between pastorally farmed foods (meat and dairy), arable crops, horticulture and seafood. The reality is that, when we eat a meal, it is almost invariably a mixture of different food types. Food ingredients sourced from different sectors may be combined into a single foodstuff, different foods from different sectors will be on the plate for a single course and different foods will make up the different courses of a meal, usually with very different food make-up. When we eat food, it all gets mechanically broken down and mixed, ending up in the same place – initially the stomach. Recently, attention has begun to be paid to the interactions of foods of different types when consumed together. When two foods interact during digestion to behave differently from how they would be digested separately, this is called ‘food synergy’ – the whole differs from the sum of the parts. Kiwifruit is an interesting food because it contains a very active proteolytic enzyme – actinidin. In support of ZESPRI’s health and nutrition research programme underpinning market initiatives, we have recently been investigating the effect of eating kiwifruit on the digestion of a range of food proteins. This work, initially carried out using an in vitro model system, has recently been validated using laboratory rats and shows that the consumption of kiwifruit together with food protein can have a dramatic effect on digestion for some food proteins but little effect on others, particularly for digestion in the stomach. Proteins that were more effectively digested in the presence of actinidin included soy protein, beef muscle protein and gluten, while little effect was seen for whey protein. Studies with rats using Massey University’s wide-bore NMR have allowed an investigation of rates of stomach emptying when rats are fed different proteins, with or without kiwifruit. Initial studies have shown that, for proteins where actinidin helps break down the food protein, stomach emptying is more rapid when kiwifruit is consumed. These results may explain the observation that, when kiwifruit is consumed as part of a high protein meal, feelings of over fullness are avoided. Breakdown of food proteins during digestion is known to give rise to a range of bioactive peptides that have a range of different functional properties. Preliminary evidence shows that consumption of kiwifruit with food proteins, in addition to enhancing digestion, changes the nature of the peptides produced. These can be expected to have altered functional properties. The Riddet Institute’s new QTOF mass spectrometer is now being used to help us understand the detailed chemistry of actinidin-assisted digestion and the peptides that are produced. The Riddet Institute acknowledges ZESPRI for financial support for this work and recognises ZESPRI’s ongoing role as a cornerstone industry partner. Riddet Institute Centre of Research Excellence Annual Report 2009 25 Our science Personalised Nutrition – ‘I’-foods We have a highly original approach to the delivery of innovative foods and beverages customised for the individual and manufactured at point of sale. It brings together a truly multi-disciplinary team consisting of researchers with expertise in food engineering, computer engineering and robotics, food ingredient formulations and physical functionality, nutrient utilisations/functional foods and human/clinical nutrition. P ersonalised nutrition is a response to differences between individuals and attempts to balance an individual’s diet to their specific individual health and situational needs. Nutrition is not just about a balance of macro- and micronutrients – a plethora of ‘functional’ food components are also known to affect health in ways that extend far beyond the simple supply of nutrients, and they can be modifiers of nutrient uptake and usage as well as regulators of the expression of some genes, thus modifying the effect of nutritional balance as seen by the body’s metabolism. Personalised nutrition attempts to take this into account, to provide optimal customised nutrition for each individual. Personalised nutrition is widely seen as a delivery vehicle for the benefits of nutrigenomics. In sophisticated markets today, there is wide acceptance that nutrition has a profound effect on health and wellness, and as individuals become more aware of their specific nutritional needs, the demand for personalised nutrition is expected to increase. Personalised nutrition goes beyond the appeal of the expected health benefits – it appeals to the sense of self as an individual. This sense has been led by Apple with its iPod. Individualised products are successful because they appeal to today’s sophisticated consumers and allow them to feel empowered and distinguished from the crowd. It is instructive to look at personalisation in the context of Maslow’s hierarchy of needs. One version of Maslow’s pyramid is shown here. The principle of the pyramid is that needs on a lower level must be met before needs at the level above are addressed. In affluent society, all of the survival needs are easily met for the majority of the population. It is the ‘self-actualisation’ drivers in the higher levels that drive the consumer. Personalised nutrition is all about meeting these higher-level needs in the context of nutrition. Methods for the delivery of personalised nutrition will need to evolve. We can already see a range of specialist dietary food products on supermarket shelves, including fat-free, gluten-free, low-starch, enriched with omega-3 fats and so on. 26 Riddet Institute Centre of Research Excellence Annual Report 2009 Partial solutions, based on smart ingredients and selected (and eventually specially bred) fresh foods, and targeting the most common nutrition-affecting genetic variations will be early products to market. The POSIFoods concept, a recently completed project involving the Riddet Institute with Fonterra and BASF, Germany, was an attempt to marry personalised nutrition with convenience to better meet consumer needs. Development of this concept – or any other serious form of personalised nutrition – has yet to occur. This is partly because the necessary outputs from nutrigenomics are as yet incomplete and partly because the appropriate business model has yet to be developed. The Riddet Institute continues to develop its ideas around personalised nutrition, with a new concept presently under development. drivers for ‘i’ Beauty Knowledge Self-actualisation Esteem Belongingness survival needs Safety Physiological Maslow’s hierarchy of needs (Boland, 2006). Awards and achievements Dr Aiqian Ye Professor Andrew Pullan Professor Roger Lentle Professor Laurie Melton Professor Harjinder Singh Distinguished Professor Paul Moughan was awarded the Massey University Research Medal – Early Career. He was also a finalist in the 2009 Agricultural Food and Chemistry Society Young Scientist Awards held in Washington DC. Principal Investigator Professor Laurie Melton was appointed Distinguished Visiting Scientist of Food Science Australia at Werribee. Principal Investigator Professor Andrew Pullan was elected a Fellow of the Royal Society of New Zealand. Professor Pullan is based at the University of Auckland. was appointed a Guest Professor at Kumamoto University in Japan. Professor Geoffrey Jameson PhD scholar Anwesha Sarkar A Marsden grant ($860,000 over three years) was awarded to Principal Investigator Professor Geoffrey Jameson. received the 6th NIZO Elsevier Young Scientist Award for the best presentation at the NIZO Conference held in the Netherlands in October. Associate Investigator Roger Lentle was promoted to Professor. He is based at Massey University. advised on Canadian Research Chair appointments in his capacity as an appointed member of the College of Reviewers for Canadian Research Chairs. He also chaired an international committee to review postgraduate research and teaching at Wageningen University in the Netherlands. Riddet Institute Centre of Research Excellence Annual Report 2009 27 Statement of financial performance (CoRE activities) for the period ended 31 December 2009 INCOME Revenue NOTES TEC operational funding Earle Food Research Fund Interest received (scholarship fund) 1 Sundry income and reimbursements Total revenue Funds carried forward 2008–2009 Funds carried forward 2009–2010 2008 2009 2,134,666 3,134,660 1,000,000 0 84,000 0 0 21,721 3,218,666 3,156,381 Earle Food Research Fund 0 1,084,000 Operational Funding 0 1,043,352 Total funds carried forward 2008–2009 0 2,127,352 -1,084,000 -1,071,025 Earle Food Research Fund 2 Operational funding -1,043,352 -810,218 Total funds carried forward 2009–2010 -2,127,352 -1,881,243 1,091,314 3,402,490 TOTAL INCOME EXPENDITURE Research platforms Administration and infrastructure Overheads Platform research costs (incl. overheads) 3 288,670 2,453,088 Earle Food Research Fund scholarships 2 0 12,975 Total research platforms 288,670 2,466,063 Staff-related costs 496,984 467,532 148,569 324,914 Total administration and infrastructure Operational costs 645,553 792,446 Massey University overheads (administration) 157,091 143,981 1,091,314 3,402,490 TOTAL EXPENDITURE 4 Notes: 1 Interest for 2009 to be credited in 2010. 2 First scholarship funding round held in 2009. Six scholarships awarded with students starting late 2009 and early 2010. 3 Payments to partners and collaborators. Research programme started July 2008 (six months operation only). 4 Includes $120,000 for Massey University space charges and costs for meetings (Principal Investigator meetings, research platform meetings, Board meetings) and events (student colloquium, Riddet Institute conference, Future of Food summit). 28 Riddet Institute Centre of Research Excellence Annual Report 2009 Notes to the financial statements Entity reporting From the first year of TEC CoRE funding, $1 million was set aside for a scholarship fund (the Earle Food Research Fund), which is used to support students working on the CoRE research programme over the term of the TEC CoRE contract. Massey University has agreed to invest this funding in order to accrue interest. These financial reports are for the Riddet Institute, a Centre of Research Excellence established in 2008 consequent to an agreement with the Tertiary Education Commission (TEC) for funding and an agreement between Massey University, AgResearch Ltd, the New Zealand Institute for Crop & Food Research Ltd and the University of Otago for its operation. Expenditure The Riddet Institute is hosted by Massey University, and these financial reports have been generated from Massey University financial records, which are audited annually. These financial reports cover income and expenditure for the Riddet Institute CoRE budget centre, but do not include income and expenditure related to non-CoRE activities in the Riddet Institute. A large proportion of the Riddet Institute’s expenditure related to the CoRE research programme is by partners and collaborators under research subcontracts. This expenditure is recognised in the Statement of financial performance as payments to those institutions rather than expenditure incurred by them. Reporting period Goods and ServiceS Tax (GST) These reports are for income and expenditure in the 12 months ending 31 December 2009. These financial reports have been prepared so that all figures are stated exclusive of GST. Income Revenue is recognised in the Statement of Financial Performance as that actually received. Income is then adjusted to account for research activity completed in advance or yet to be completed. Capital equipment purchased (from TEC Capital Equipment Grant) For period ended 31 December 2009 NOTES NZ$ Capital Equipment Grant from TEC 1,630,590 Capital equipment purchased in 2008 1,028,272 602,318 Funds brought forward from 2008 Items Purchased 2009 Contribution towards SAXS camera Cost 1 100,000 QTOF mass spectrometer 2 256,063 High-end computer 1 Ultra high pressure unit 97,611 Funds carried forward to 2010 20,212 473,686 Total 2 128,432 Notes: 1 All capital equipment listed above is to be located at the Riddet Institute at Massey University, Palmerston North except: • SAXS camera Victoria University • high-end computer The University of Auckland 2 $103,306 outstanding on mass spectrometer purchase to be paid in 2010. Uncommitted funds at year end 2009: $25,126. Riddet Institute Centre of Research Excellence Annual Report 2009 29 Scientific publications Peer-reviewed journal publications Awati, A., Rutherfurd, S.M., Plugge, W., Reynolds, G.W., Marrant, H., Kies, A.K., and Moughan, P.J. (2009). ‘Ussing chamber results for amino acid absorption of protein hydrolysates in porcine jejunum must be corrected for endogenous protein.’ Journal of the Science of Food and Agriculture, 89, 1857-1861. Awati, A., Rutherfurd, S.M., Kies, A.K., Veyry, A., and Moughan, P.J. (2009). ‘Endogenous lysine in ileal digesta in the growing rat determined using different methods.’ Journal of the Science of Food and Agriculture, 89, 2200-2206. Balan, P., Han, K.S., Rutherfurd, S.M., Singh, H., and Moughan, P.J. (2009). ‘Orally administered ovine serum immunoglobulins influence growth performance, organ weights and gut morphology in growing rats.’ The Journal of Nutrition, 139, 244-249. Booten, T.J., Harris, P.J., Melton, L.D., and Newman, R.H. (2009). ‘Solid-state 13C NMR study of a composite of tobacco xyloglucan and Gluconacetobacter xylinus cellulose: Molecular interactions between the component polysaccharides.’ Biomacromolecules, 10, 2961- 2967. Chase, J.G., Andreassen, S., Pielmeier, U., Hann, C.E., McAuley, K.A., and Mann, J.I. (2009). ‘A glucose-insulin pharmacodynamic surface modeling validation and comparison of metabolic system models.’ Biomedical Signal Processing and Control, (BSPC), 4 (4), 355-363, ISSN: 1746-8094 (invited special edition). Chung Chun Lam, S.M.S., Moughan, P.J., Awati, A., and Morton, H.R. (2009). ‘The influence of whey protein and glycomacropeptide on satiety in adult humans.’ Physiology and Behaviour, 96, 162-168. Cochrane, F.C., Cookson, T.V.M., Jameson, G.B., and Parker, E.J. (2009). Reversing evolution: re-establishing obligate metal-ion dependency in a metal-independent KDO8P synthase.’ Journal of Molecular Biology, 390, 646-661. Cucheval, A S.B., Al-Gobashy, M., Hemar, Y., Otter, D., and Williams, M.A.K. (2009). ‘Direct measurements of interfacial interactions between pectin and k-casein and implications for the stabilization of calcium-free casein micelle mimics.’ Journal of Colloid and Interface Science, 338 (2), 450-462. Cucheval, A.S.B., Vincent, R.R., Hemar, Y., Otter, D., and Williams, M.A.K. (2009). ‘Diffusing wave spectroscopy investigations of acid milk gels containing pectin.’ Colloid and Polymer Science, 287 (6), 695-704. Cucheval, A.S.B., Vincent, R.R., Hemar, Y., Otter, D., and Williams, M.A.K. (2009). ‘Multiple particle tracking investigations of acid milk gels using tracer particles with designed surface chemistries and comparison with diffusing wave spectroscopy studies.’ Langmuir, 25 (19), 11827-11834. Cummings, J.H., Mann, J.I., Nishida, C., and Vorster, H.H. (2009). ‘Dietary fibre: an agreed definition.’ The Lancet, 373, 365–366. Dale, K., McAuley, K.A., Taylor, R.W., Williams, S.M., Farmer, V.L., Hansen, P., Vorgers, S.M., Chisholm, A.W., and Mann, J.I. (2009). ‘Determining optimal approaches for successful weight maintenance: A 2 x 2 factorial randomised controlled trial.’ Canadian Medical Association Journal, 180 (10): E39-E46. Dale, K.S., Mann, J.I., McAuley, K.A., Williams, S.M., and Farmer, V.L. (2009). ‘Sustainability of lifestyle changes following an intensive lifestyle intervention in insulin resistant adults: Follow-up at 2-years.’ Asia Pacific Journal of Clinical Nutrition, 18 (1): 114-20. Deglaire, A., Bos, C., Tomé, D., and Moughan, P.J. (2009). ‘Ileal digestibility of dietary protein in the growing pig and adult human.’ British Journal of Nutrition, 102, 1752-1759. Deglaire, A., Fromentin, C., Fouillet, H., Airinei, G., Gaudichon, C., Boutry, C., Benamouzig, R., Moughan, P.J., Tome, D., and Bos, C. (2009). ‘Hydrolyzed dietary casein as compared with the intact protein reduces postprandial peripheral, but not whole-body, uptake of nitrogen in humans.’ American Journal of Clinical Nutrition, 90, 1011-1022. 30 Riddet Institute Centre of Research Excellence Annual Report 2009 Dekker, J. W., Wickens, K., Black, P. N., Stanley, T. V., Mitchell, E. A., Fitzharris, P., Tannock, G. W., Purdie, G., and Crane, J. (2009). ‘Safety aspects of probiotic bacterial strains Lactobacillus rhamnosus HN001 and Bifidobacterium animalis subsp. lactis HN019 in human infants aged 0-2 years.’ International Dairy Journal, 19, 149-154. Devenish, S.R.A. and Gerrard, J.A. (2009). ‘The role of quaternary structure in (ß/a)8-barrel proteins: evolutionary happenstance or a higher level of structure-function relationships?’ Organic and Biomolecular Chemistry, 7, 833-839. Dobson, R.C.J., Perugini, M.A., Jameson, G.B., and Gerrard, J.A. (2009). ‘Specificity versus catalytic potency: The role of threonine 44 in Escherichia coli dihydrodipicolinate synthase mediated catalysis.’ Biochimie, 91, 1036-1044. Du, P., O’Grady, G., Egbuji, J.U., Lammers, W.J., Budgett, D., Nielsen, P., Windsor, J.A., Pullan, A.J., and Cheng, L.K. (2009). ‘High-resolution mapping of in vivo gastrointestinal slow wave activity using flexible printed circuit board electrodes: methodology and validation.’ Annals of Biomedical Engineering, 37 (4), 839-846. Du, P., Li, S., O’Grady, G., Cheng, L.K., Pullan, A.J., and Chen, J.D.Z. (2009). ‘Effects of electrical stimulation on isolated rodent gastric smooth muscle cells evaluated via a joint computational simulation and experimental approach.’ American Journal of Physiology - Gastrointestinal and Liver Physiology, 297, G672-G680. Du, P., O’Grady, G., Windsor, J.A., Cheng, L.K., and Pullan, A.J. (2009). ‘A tissue framework for simulating the effects of gastric electrical stimulation and in-vivo validation.’ IEEE Transactions on Biomedical Engineering, 56 (12), 2755-2761. Faville, R.A., Pullan, A.J., Sanders, K.M., Koh, S.D., Lloyd, C.M., and Smith, N.P. (2009). ‘Biophysically-based mathematical modelling of interstitial cells of Cajal slow wave activity generated from a discrete unitary potential basis.’ Biophysical Journal, 96 (12) 4834-4852. Fellahm A., Anjukandi, P., Waterland, M.R., and Williams M.A.K. (2009). ‘Determining the degree of methylesterification of pectin by ATR/FT-IR: Methodology optimisation and comparison with theoretical calculations.’ Carbohydrate Polymers, 78 (4), 847-853. Ferrua, M.J. and Singh, R.P. (2009). ‘Modeling the forced-air cooling process of fresh strawberry packages. Part I: numerical model.’ International Journal of Refrigeration, 32 (2), 335-348. Ferrua, M.J. and Singh, R.P. (2009). ‘Modeling the forced air cooling process of fresh strawberry packages. Part II: Experimental validation of the flow model.’ International Journal of Refrigeration, 32 (2), 349-358. Ferrua, M.J. and Singh, R.P. (2009). ‘Modeling the forced-air cooling process of fresh strawberry packages. Part III: Experimental validation of the energy model.’ International Journal of Refrigeration, 32 (2), 359-368. Ferrua M.J. and Singh, R.P. (2009). ‘Design guidelines for the forced-air cooling process of strawberries.’ International Journal of Refrigeration, 32 (8), 1932-1943. Fraser, R.D.B. and Parry, D.A.D. (2009). ‘The role of Beta-Sheets in the structure and assembly of keratins.’ Biophysical Reviews, 1, 27-35. Garvey, M., Gras, S. L., Meehan, S., Meade, S.J., Carver, J.A., and Gerrard, J.A. (2009). ‘Protein nanofibres of defined morphology prepared from mixtures of crude crystallins.’ International Journal of Nanotechnology, 6, 258-273. Han, K.-S., Boland, M., Singh, H., and Moughan, P.J. (2009). ‘The in vitro anti-pathogenic activity of immunoglobulin concentrates extracted from ovine blood.’ Applied Biochemistry and Biotechnology, 157, 442-452. Hindmarsh, J. (2009). ‘Investigation of drying and re-hydration of powdered dairy products with magnetic resonance imaging getting home with a FRST Fellowship.’ New Zealand Science Review, 66 (2), 74. Hutchings, S.C., Bronlund, J.E., Lentle, R.G., Foster, K.D., Jones, J.R., and Morgenstern, M.P. (2009). ‘Variation of bite size with different types of food bars and implications for serving methods in mastication studies.’ Food Quality and Preference, 20 (6), 456-460. Janssen, P.W.M., Lentle, R.G., Hulls, C., Ravindran, V., and Amerah, A.M. (2009). ‘Spatiotemporal mapping of the motility of the isolated caecum of the chicken.’ Journal of Comparative Physiology B, 179 (5), 593-604. Kaur, L., Singh, J., and Singh, H. (2009). ‘Characterization of Gum Ghatti (Angeissus latifolia): A structural and rheological approach.’ Journal of Food Science, 72, E328-E332. Kaur, L., Singh, J., Singh, H., and McCarthy, O.J. (2009). ‘Cassia Gum: A novel galactomannan for use in starch based foods.’ Getreidetechnologie, 63 (4), 11-25. Kemp, M., Edwards, B., Burgess, M., Clarke, W.E., Nicholson, G., Parry, D.A.D., and Davies, K.E. (2009). ‘Syncoilin isoform organisation and differential expression in murine striated muscle.’ Journal of Structural Biology, 165, 196-203. Kim, Y., Kim, M.J., Han, K.S., Imm, J.Y., Oh, S., and Kim, S.H. (2009). ‘Anticancer activity of lactoferrin isolated from caprine colostrums on human cancer cell lines.’ International Journal of Dairy Technology, 62 (2), 277-281. Knoch, B., Barnett, M.P.G., Roy, N.C., and McNabb, W.C. (2009). ‘Review: Study of the effects of dietary polyunsaturated fatty acids: Molecular mechanisms involved in intestinal inflammation.’ International Journal of Fats and Oils, 60, 8-21. Knoch, B., Barnett, M.P.G., Zhu, S.T., Park, Z.A., Nones, K., Dommels, Y.E.M., Knowles, S.O., McNabb, W.C., and Roy, N.C. (2009). ‘Genome-wide analysis of genes involved in dietary eicosapentaenoic acid- and oleic acid-induced modulation in colon inflammation of interleukin 10 gene deficient mice.’ Journal of Nutrigenetics and Nutrigenomics, 2, 9-28. Kong, F. and Singh, R.P. (2009). ‘Modes of disintegration of solid foods in simulated gastric environment.’ Food Biophysics, 4 (3), 180-190. Kong, F. and Singh, R.P. (2009). ‘Digestion of raw and roasted almonds in simulated gastric environment.’ Food Biophysics, 4 (4), 365-377. Kuhn, J., Delahunty, C.M., Considine, T., and Singh, H. (2009). ‘In-mouth flavour release from milk proteins.’ International Dairy Journal, 19, 307-313. Lentle, R.G. and Janssen, P. (2009). ‘A review of the roles of filtration and expression in the processing of digesta with high solid phase content.’ Comparative Biochemistry and Physiology – Part A, 154 (1), 1-9. Loveday, S.M., Hindmarsh, J.P., Creamer, L.K., and Singh, H. (2009). ‘Physicochemical changes in a model protein bar during storage.’ Food Research International, Elsevier, 798-806. Loveday, S.M., Rao, M.A., Creamer, L.K., and Singh, H. (2009). ‘Review: Factors affecting rheological characteristics of fibril gels: The case of ß-lactoglobulin and a-lactalbumin.’ Journal of Food Science, 74, 47-55. Lundin, L. and Golding, M. (2009). ‘Structure design for healthy food.’ Australian Journal of Dairy Technology, 64, 68-74. McGillivray, D.J., Mata, J.P., Zank, J., and White, J.W. (2009). ‘Nano- and microstructure of the interfaces between air, oil and water.’ Langmuir, 25, 4065. Mackintosh, S.H., Meade, S.J., Healy, J.P., Sutton, K.H., Larsen, N.G., Squires, A.M., and Gerrard, J.A. (2009). ‘Wheat glutenin proteins assemble into a nanostructure with unusual structural features.’ Journal of Cereal Science, 49, 157-162. Mallassagne-Bulgarelli, N. and McGrath, K.M. (2009). ‘Dynamics of oil transfer in oil-in-water emulsions.’ Soft Matter, 5, 4804-4813. Mann, J. (2009). ‘Alcohol and type 2 diabetes.’ International Diabetes Monitor, 21, 35-36. Mann, J. (2009). ‘Intakes of fruit juices and sugar-sweetened beverages and risk of type 2 diabetes.’ International Diabetes Monitor, 21, 193-195. Mann, J. (2009). ‘Vegetarian diets.’ British Medical Journal, 339, b2507. Mann, J. and Nye, E.R. (2009). ‘Fad diets in Sweden, of all places.’ The Lancet, 374, 767-765. Mann, J.I. and Cummings, J.H. (2009). ‘Possible implications for health of the different definitions of dietary fibre.’ Nutrition, Metabolism and Cardiovascular Diseases, 19, 226-229. Melton, L.D., Smith, B.G., Ibrahim, R., and Schroeder, R. (2009). ‘Mannans in primary and secondary plant cell walls.’ New Zealand Journal of Forestry Science, 39, 153-160. Miner-Williams, W., Moughan, P.J., and Fuller, M.F. (2009). ‘Endogenous components of digesta protein from the terminal ileum of pigs fed a casein-based diet.’ Journal of Agricultural and Food Chemistry, 57, 2072-2078. Miner-Williams, W., Moughan, P.J., and Fuller, M.F. (2009). ‘Methods for mucin analysis: a comparative study.’ Journal of Agricultural and Food Chemistry, 57, 6029-6035. Molan, A.L., Flanagan, J., Wei, W., and Moughan, P.J. (2009). ‘Selenium-containing green tea has higher antioxidant and prebiotic activities then regular green tea.’ Food Chemistry, 114, 829-835. Montoya, C.A. and Leterme, P. (2009). ‘Determination of the digestible energy and prediction of the net energy content of toasted and non-toasted canola meals from Brassica junceae and Brassica napus in growing pigs by the total faecal collection and the indigestible marker method.’ Canadian Journal of Animal Science, 89, 481-487. Montoya, C.A., Lallès, J.P., Beebe, S., Souffrant, W.B., Molle, D., and Leterme, P. (2009). ‘Susceptibility of phaseolin (Phaseolus vulgaris) subunits to trypsinolysis and influence of dietary level of raw phaseolin on protein digestion in the small intestine of rats.’ British Journal of Nutrition, 101, 1324-1332. Murti, R.A., Paterson, A.H.J., Pearce, D.L., and Bronlund, J.E. (2009). ‘Stickiness of skim milk powder using the particle gun technique.’ International Dairy Journal, 19 (3), 137-141. Noisuwan, A., Hemar, Y., Wilkinson, B., and Bronlund, J.E. (2009). ‘Dynamic rheological and microstructural properties of normal and waxy rice starch gels containing milk protein ingredients.’ Starch, 61 (3-4), 214-227. Nones, K., Dommels, Y.E.M., Barnett, M.P.G., McNabb, W.C., Park-Ng, Z.A., Sheridan, S., and Roy, N.C. (2009). ‘Effect of dietary curcumin and rutin on colonic inflammation and gene expression in mdr1a-/- mice: a model of inflammatory bowel diseases.’ British Journal of Nutrition, 101, 169-181. Nones, K., Dommels, Y.E.M., Barnett, M.P.G., McNabb, W.C., Park-Ng, Z.A., Sheridan, S., and Roy, N.C. (2009). ‘Multidrug resistance gene deficient mice have an altered caecal microflora that precedes the onset of intestinal inflammation.’ Journal of Applied Microbiology, 107 (2), 557-566. O’Grady, G., Du, P., Egbuji, J.U., Wahabi, A., Lammers, W.J.E.P., Cheng, L.K., Pullan, A.J., and Windsor, J.A. (2009). ‘A novel laparoscopic device for the measurement of gastrointestinal slow wave activity.’ Surgical Endoscopy, 23 (12), 2842-2848. Paterson, A.H.J. and Bronlund, J.E. (2009). ‘The practical implications of temperature induced moisture migration in bulk lactose.’ Journal of Food Engineering, 91 (1), 85-90. Poulsen, R.C., Du Loots, T., Moughan, P.J., and Kruger, M.C. (2009). ‘Ileal and faecal digestibility of daidzein and genistein and plasma bioavailability of these isoflavones and their bioactive metabolites in the ovariectomised rat.’ Molecular Nutrition and Food Research, 53, S27-S35. Riddet Institute Centre of Research Excellence Annual Report 2009 31 Scientific publications (continued) Ravindran, V., Morel, P.C.H., Rutherfurd, S.M., and Thomas, D.V. (2009). ‘Endogenous flow of amino acids in the avian ileum as influenced by increasing dietary peptide concentrations.’ British Journal of Nutrition, 101, 822-828 Rutherfurd, S.M. (2009). ‘Accurate determination of the amino acid content of selected feedstuffs.’ International Journal of Food Sciences and Nutrition, 60 Suppl. 7, 53-62. Rutherfurd, S.M. and Sarwar-Gilani, G. (2009). ‘Review: Amino Acid Analysis.’ Current Protocols in Protein Science, 58, 11.9.1-11.9.37. Sarkar, A., Goh, K.K.T., and Singh, H. (2009). ‘Colloidal stability and interactions of milk-protein-stabilized emulsions in an artificial saliva.’ Food Hydrocolloids, 23, 1270-1278. Sarkar, A., Goh, K.K.T., Singh, R.P., and Singh, H. (2009). ‘Behaviour of an oil-in-water emulsion stabilized by ß-lactoglobulin in an in vitro gastric model.’ Food Hydrocolloids. 23, 1563-1569. Schroeder, R., Melton, L.D., Harris, P.J., Smith, B.G., and Schmitt, U. (2009). ‘Plant cell walls: diversity and approaches to understanding their function.’ New Zealand Journal of Forestry Science, 39, 113-114. Seimon, R.V., Wooster, T., Otto, B., Golding, M., Day, L., Little, T.J., Horowitz, M., Clifton, P.M., and Feinle-Bisset, C. (2009). ‘The droplet size of intraduodenal fat emulsions influences antropyloroduodenal motility, hormone release, and appetite in healthy males.’ American Journal of Clinical Nutrition, 89, 1729-1736. Sensula, B.M., Derrick, P.J., Bickerton, J.C., and Pazdur, A. (2009). ‘Mass spectrometric study of glucose and cellobiose produced during enzymatic hydrolysis of a-cellulose extracted from oak late-wood annual rings.’ Rapid Communications in Mass Spectrometry, 23 (13), 2070-2074. Singh, H., Ye, A., and Horne, D. (2009). ‘Review: Structuring food emulsion in the gastrointestinal tract to modify lipid digestion.’ Progress in Lipid Research, 28, 92-100. Singh, J., Dartois, A., and Kaur, L. (2009). ‘Review: Starch digestibility in food matrix.’ Trends in Food Science and Technology, (available online) doi:10.1016/j.tifs.2009.12.001. Singh, J., Kaur, L., McCarthy, O.J., Moughan, P.J., and Singh, H. (2009). ‘Development and characterization of extruded snacks from New Zealand Taewa (Maori potato) flours.’ Food Research International, 42, 666-673. Stanger, J., Tucker, N., Wallace, A., Larsen, N., Staiger, M., and Reeves, R. (2009). ‘The effect of electrode configuration and substrate material on the mass deposition rate of electrospinning.’ Journal of Applied Polymer Science, 112, 1729–1737. Sugiarto, M., Ye, A., and Singh, H. (2009). ‘Characterisation of binding of iron to sodium caseinate and whey protein isolate.’ Food Chemistry, 114, 1007-1013. Sukumar, D., Vidyarthi, S.K., and Singh, R.P. (2009). ‘Impact of blending of frying oils on viscosity and heat transfer coefficient at elevated temperatures.’ Journal of Food Processing and Preservation, DOI 10.1111/j.1745-4530.2008.00265.x. Sun-Waterhouse, D., Chen, J., Chuah, C., Wibisono, R., Melton, L.D., Laing, W., Ferguson, L.R., and Skinner, M.A. (2009). ‘Kiwifruitbased polyphenols and related antioxidants for functional foods: kiwifruit extract-enhancing gluten-free bread.’ International Journal of Food Sciences and Nutrition, 22, 1-14. Sun-Waterhouse, D., Wen, I., Wibisono, R., Melton, L.D., and Wadwha, S. (2009). ‘Evaluation of the extraction efficiency for polyphenol extracts from by-products of green kiwifruit juicing.’ International Journal of Food Science and Technology, 44, 2644-2652. Sutherland, J., Miles, M.C., Hedderley, D., Li, J., Devoy, S., Sutton, K., and Lauren, D. (2009). ‘In vitro effects of food components on selected probiotic and pathogenic bacteria.’ International Journal of Food Sciences and Nutrition, 60 (8), 717-727. Tannock, G.W. (2009). ‘Review: Research for the 21st century: can we draw a blueprint of the bowel ecosystem?’ Bioscience and Microflora, 28, 75-80. 32 Riddet Institute Centre of Research Excellence Annual Report 2009 Thimm, J.C., Burritt, D.J., Ducker, W.A., and Melton, L.D. (2009). ‘Pectins influence microfibril aggregation in celery cell walls: An atomic force microscopy study.’ Journal of Structural Biology, 168, 337-344. Thompson, A.K., Couchoud, A., and Singh, H. (2009). ‘Comparison of hydrophobic and hydrophilic encapsulation using liposomes prepared from milk fat globule-derived phospholipids and soya phospholipids.’ Dairy Science and Technology, 89, 99-113. Vincent, R.R. and Williams, M.A.K. (2009). ‘Microrheological investigations give insights into the microstructure and functionality of pectin gels.’ Carbohydrate Research, 344 (14), 1863-1871. Vincent, R.R., Cucheval, A., Hemar, Y., and Williams, M.A.K. (2009). ‘Bio-inspired network optimization in soft materials Insights from the plant cell wall.’ The European Physical Journal, 28 (1), 79-87. Werner, S.R.L., Jones, J.R., Paterson, A.H.J., Archer, R.H., and Pearce, D.L. (2009). ‘Droplet impact and spreading on lecithinated anhydrous milkfat surfaces.’ Journal of Food Engineering, 90, 525-530. White, J.W., Perriman, A.W., McGillivray, D.J., and Lin, J.M. (2009). ‘Protein interfacial structure and nanotoxicology.’ Nuclear Instruments and Methods in Physics Research, 600 (1), 263-265. Williams, M.A.K., Cucheval, A., Ström, A., and Ralet, M. (2009). ‘Electrophoretic behaviour of co-polymeric galacturonans including comments on the information content of the intermolecular charge distribution.’ Biomacromolecules, 10 (6), 1523-1531. Xie, D., Xu, W.L., Foster K.D., and Bronlund, J. (2009). ‘Objectoriented knowledge framework for modelling human mastication of foods.’ Expert Systems with Applications, 36 (3), 4810-4821. Xu, W.L,. Fang, F., Bronlund, J., and Potgieter, J. (2009). ‘Generation of rhythmic and voluntary pattern of mastication using Matsouka oscillator.’ Mechatronics, 19 (2), 205-217. Ye, A., Cui, J., Taneja, A., Zhu, X., and Singh, H. (2009). ‘Evaluation of processed cheese fortified with fish oil emulsion.’ Food Research International, 42, 1093-1098. Ye, A. and Hewitt, S. (2009). ‘Phase structures impact the rheological properties of rennet-casein-based imitation cheese containing starch.’ Food Hydrocolloids, 23, 867-873. Ye, A., Hewitt, S., and Taylor, S. (2009). ‘Characteristics of rennet-casein-based model processed cheese containing maize starch: rheological properties, meltabilities and microstructures.’ Food Hydrocolloids, 23, 1220-1227. Ye, A. and Taylor, S. (2009). ‘Characterization of cold-set gels produced from heated emulsions stabilized by whey protein.’ International Dairy Journal, 19, 721-727. Younghoon, K., Mae, J.K., Kyoung, S.H., Jee, Y.I., Sejong, O., and Sae, H.K. (2009). ‘Anticancer activity of lactoferrin isolated from caprine colostrum on human cancer cell lines.’ International Journal of Dairy Technology, 26, 277-281. Book Edited Singh, J. and Kaur, L. (2009). Advances in Potato Chemistry and Technology. Elsevier/Academic Press, USA. Singh, R.P. and Erdogdu, F. (2009). Virtual Experiments in Food Processing. 2nd edition. RAR Press, Davis, CA. Singh, R.P. and Heldman, D.R. (2009). Introduction to Food Engineering. 4th edition. Academic Press, London. Book Chapters Johns, M.L., Wilson, D.I., and Hindmarsh, J.P. (2009). ‘Magnetic resonance studies of drop-freezing processes.’ In: Magnetic Resonance Microscopy: Spatially Resolved NMR Techniques and Applications. Codd, S.L. and Seymour, J.D. (Eds). John Wiley and Sons, pp. 465-486. Kaur, L. and Singh, J. (2009). ‘The role of galactomannan seed gums in diet and health – a review.’ In: Recent Progress in Medicinal Plants, Vol 24, Standardization of Herbal/Ayurvedic Formulations. Govil, J.N. and Singh, V.K. (Eds). Studium Press LLC, USA, pp. 329-343. Kong, F. and Singh, R.P. (2009). ‘Emerging Food Technologies’. In: Process-Induced Food Toxicants: Occurrence, Formation, Mitigation, and Health Risks. Stadler, R.H. and Lineback, D.R. (Eds). John Wiley and Sons, pp. 621-643. Liu, Q., Donner, E., Tarn, R., Singh, J., and Chung, H. (2009). ‘Advanced analytical techniques to evaluate the quality of potato and potato starch.’ In: Advances in Potato Chemistry and Technology. Singh, J. and Kaur, L. (Eds). Elsevier, pp. 221-248. McCarthy, O.J. and H. Singh (2009). ‘Physico-chemical properties of milk.’ In: Advanced Dairy Chemistry -III, Lactose, water, salts and minor constituents. McSweeny, P.L.H. and Fox, P. F. (Eds). Springer, New York, pp. 691-759. Moughan, P.J. (2009). ‘Digestion and absorption of proteins and peptides.’ In: Designing Functional Foods: Measuring and Controlling Food Structure Breakdown and Nutrient Absorption.McClements, D.J. and Decker. E.A. (Eds). Woodhead Publishing, pp. 148-170. Singh, H. (2009). ‘Protein interactions and functionality of milk protein products.’ In: Dairy-derived ingredients. Food and nutraceutical uses. Corredig, M. (Ed). Woodhead Publishing, Oxford, pp. 644-674. Singh, H., Ye, A., and Thompson, A. (2009). ‘Nanoencapsulation systems based on milk proteins and phospholipids’. Chapter 8. In: ACS Symposium Series, Vol.1007, pp. 131-142. Singh, J. and Kaur, L. (2009). ‘Introduction.’ In: Advances in Potato Chemistry and Technology. Singh, J. and Kaur, L. (Eds). Elsevier, pp. ix-xii. Singh, J. and Kaur, L. (2009). ‘Novel applications and non-food uses of potato: future perspectives in nanotechnology. Chapter 15. In: Advances in Potato Chemistry and Technology, Singh, J. and Kaur, L. (Eds). Elsevier, pp. 425-446. Singh, J., Kaur, L., and McCarthy, J. (2009). ‘Potato starch and its modification.’ In: Advances in Potato Chemistry and Technology, Singh, J. and Kaur, L. (Eds). Elsevier, pp. 273-318. Singh, J., Kaur, L., and Rao, M.A. (2009). ‘Textural and Rheological Characteristics of Raw and Cooked Potatoes. In: Advances in Potato Chemistry and Technology, Singh, J. and Kaur, L. (Eds). Elsevier, pp. 249-272. Singh, R.P. (2009). ‘Numerical Procedures.’ Chapter 7. In: Food Science and Technology. Campbell-Platt, G. (Ed). Wiley-Blackwell, Ames, IA, pp. 175-192. Singh, R.P. (2009). ‘Food Engineering.’ Chapter 10. In: Food Science and Technology. Campbell-Platt, G. (Ed). Wiley-Blackwell, Ames, IA, pp. 247-278. Singh, R.P., Erdogdu, F., and Rahman, M.S. (2009). ‘Specific Heat and Enthalpy of Foods.’ Chapter 16. In: Food Properties Handbook. 2nd edition. Rahman, M.S. (Ed). CRC Publishers, Boca Raton, pp. 517-543. Tannock, G.W. (2009). ‘Microbial succession in gut health: probiotics.’ In: Foodborne microbes: shaping the host ecosystem. Jaykus, L-A., Wang, H.H., and Schlesinger, L.S. (Eds). ASM Press, Washington, DC, USA, pp. 63-79 [ISBN 978-1-55581-405-2]. Tannock, G.W. (2009). ‘What pediatricians need to know about the analysis of the gut microbiota.’ In: Probiotics and pediatric medicine. Michial, S. and Sherman, P.M. (Eds). Humana Press, Totawa, NJ, USA, pp. 17-28 [ISBN 978-1-60327-288-9]. Keynote and invited addresses Monro, J. (2009). Homeostasis and the accuracy of functional food values. Nutrition Society of Australia and Nutrition Society of New Zealand Joint Annual Scientific Meeting, Newcastle, Australia, 8-11 December. Moughan, P.J. (2009). Protein/amino acid determination of diets and ingredients. International Postgraduate Seminar Advances in Feed Evaluation Science, Wageningen, The Netherlands, 30 March-3 April. Moughan, P.J. (2009). Educating the next generation in agriculture. 25th International Animal Health and Nutrition Symposium, Kentucky, USA, 18 May. Moughan, P.J. (2009). Describing dietary energy – do we need a new approach? Nutrition Society of Australia and Nutrition Society of New Zealand Joint Annual Scientific Meeting, Newcastle, Australia, 8-11 December. Singh, H. (2009). Controlling milk protein interactions to enhance powder functionality. 4th International Symposium on Spray Dried Dairy Products, Melbourne, 15-17 April. Singh, H. (2009). Interfacial engineering of food emulsions to modify lipid digestion. The Japan Food Machinery Manufacturers’ Association Conference, Tokyo, Japan, 9-13 June. Singh, H. (2009). Food structure assembly and nutrition: status and prospects. NZIFST Conference 2009 – Food Elements: Putting the pieces together, Christchurch, 23-25 June. Singh, H. (2009). Delivery of bioactive lipids in functional foods. 28th ISF Congress, Sydney, Australia, 26-30 September. Singh, H. (2009). Structuring food materials to control nutrient bioavailability. Nutrition Society of Australia and Nutrition Society of New Zealand Joint Annual Scientific Meeting, Newcastle, Australia, 8-11 December. Ye, A. (2009). Understanding biopolymer interactions: thermodynamic approaches from model systems to real foods. 238th American Chemical Society National Meeting, Washington DC, 16-20 August. Papers in Published Conference Proceedings Anderson, R.C., McNabb, W.C., and Roy, N.C. (2009). The effects of human Lactobacillus fermentum isolates on in vitro colonic barrier integrity and epithelial cell whole genome expression are strain dependent. 6th European Nutrigenomics Conference (NuGO week), p. 156, Montecatini Terme, Italy, 31 August-3 September. Barnett, M.P.G., McNabb, W.C., and Roy, N.C. (2009). An introduction to Nutrigenomics: Tailoring New Zealand foods to match people’s genes. The Australian and New Zealand Laboratory Animal Association (ANZLAA) 2009 Annual Conference, Wellington, New Zealand, 1-3 September. Bermingham, E.N., Barnett, M.P.G., McNabb, W.C., Zhu, Z., and Roy, N.C. (2009). Epigenetic effects of post-natal supplementation of selenium and folate. 6th European Nutrigenomics Conference (NuGO week), p. 143, Montecatini Terme, Italy, 31 August-3 September. Cheng, L.K., O’Grady, G., Du, P., Egbuji, J.U., Windsor, J.A., and Pullan, A.J. (2009). Detailed Measurements of Gastric Electrical Activity and their Implications on Inverse Solutions. Proceedings of the 31st Annual IEEE Engineering and Medicine in Biology Conference, pp. 1302-1305, Minneapolis, USA, 2-6 September. Cooney, J.M., Barraclough, D., Laing, W., Barnett, M.P.G., Knoch, B., Dommels, Y.E.M., McNabb, W.C., and Roy, N.C. (2009). Differential expression of proteins in mouse models of human inflammatory bowel disease following dietary intervention with polyunsaturated fatty acid or polyphenol rich diets. 6th European Nutrigenomics Conference (NuGO week), p. 158, Montecatini Terme, Italy, 31 August-3 September. Du, P., Qiao, W., O’Grady, G., Egbuji, J.U., Lammers, Q.J., Cheng, L.K., and Pullan, A.J.(2009). Automated detection of gastric slow wave events and estimation of propagation velocity vector fields from serosal high-resolution mapping. Proceedings of the 31st Annual IEEE Engineering and Medicine in Biology Conference, pp. 2527-2530, Minneapolis, USA, 2-6 September. Riddet Institute Centre of Research Excellence Annual Report 2009 33 Scientific publications (continued) Du, P., Shiyin, L., Cheng, L.K., Pullan, A.J., and Chen, J. (2009). A virtual model for optimizing gastric electrical stimulation protocols. Conference Proceedings for Digestive Disease Week, Chicago, USA, 30 May-4 June. Du, P., O’Grady, G., Egbuji, J.U., Lammers, W., Windsor, J.A., Cheng, L.K., Pullan, A.J. (2009). A new method for the highresolution mapping of human slow wave activity using flexible printed circuit board electrodes. Conference Proceedings for Digestive Disease Week, Chicago, USA, 30 May-4 June. Egbuji, J.U., O’Grady, G.O., Du, P., Windsor, J.A., and Pullan, A.J. (2009). Mechanisms of gastric arrhythmias in the porcine model. HealthX NZ Conference, Auckland Medical School, Auckland, 11 September. Ferrua, M.J., Marra, F., and Singh, R.P. (2009). PIV analysis of the flow field within a closed system that simulates the peristaltic movement of the stomach wall. IFT Annual Meeting. No 09-A-2619IFT, Anaheim, California, 6-9 June. Gladine, C., Roy, N., Russ, A., Park Z., Morio, B., Chardigny, J. M., McNabb, W. C. and Comte, B. (2009). Modulations of aortic and hepatic transcriptomes by docosahexaenoic acid during atherosclerosis development: are there some dose/response effects? 6th European Nutrigenomics Conference (NuGO week), p. 44, Montecatini Terme, Italy, 31 August-3 September. Huang, L., Xu, W.L., Torrance, J., and Bronlund, J.E. (2009). Modeling and impedance control of a chewing robot with a 6RSS parallel mechanism. Second International Conference, ICIRA 2009, Singapore, December. Intelligent Robotics and Applications 5928, pp. 733-743. Hutchings, S.C., Foster, K.D., Bronlund, J.E., Lentle, R.G., Sun, C., Jones, J.R., and Morgenstern, M.P. (2009). Mastication of peanuts embedded in different food matrices: a pilot study. The delivery of functionality in complex food systems: Physically-inspired approaches from nanoscale to microscale. 3rd International Symposium, Wageningen, The Netherlands, 18-21 October. Hutchings, S.C., Foster, K.D., Bronlund, J.E., Lentle, R.G., Sun, C., Jones, J.R., and Morgenstern, M.P.(2009). Mastication of peanuts embedded in different food matrices: a pilot study. 3rd Annual Biomouth Symposium. Dunedin, 10-11 June, NZIFST conference, Christchurch, July. Kim, J.H.K., Bradshaw, L.A., Pullan, A.J., and Cheng, L.K. (2009). Localization of multiple gastric slow waves from non-invasive magnetic field measurements. Joint International Neurogastroenterology and Motility meeting, Chicago, USA, 27-30 August. Knoch, B., Nones, K., Barnett, M.P.G., Cooney, J.M., Barraclough, D., Laing, W.A., McNabb, W.C., and Roy, N.C. (2009). Microbiota and protein profiles of interleukin-10 gene-deficient mice are altered when fed diets enriched in n-3 and n-6 polyunsaturated fatty acids. 33rd Annual Scientific Meeting of Nutrition Society of Australia & Nutrition Society of New Zealand, p. 115, Newcastle, Australia, 8-11 December. Knoch, B., Nones, K., Barnett, M.P.G., McNabb W.C., and Roy, N.C. (2009). Microbiota profile of interleukin-10 gene-deficient mice is altered when fed diets enriched in n-3 and n-6 polyunsaturated fatty acids. 6th European Nutrigenomics Conference (NuGO week), p. 227, Montecatini Terme, Italy, 31 August-3 September. Komuro, R., Cheng, L.K., Erickson, J.C., Bradshaw, L.A., and Pullan, A.J. (2009). Validation of simulated gastric slow wave activity with magnetic field recordings. Joint International Neurogastroenterology and Motility Meeting, Chicago, USA, 27-30 August. Loveday, S.M., Wang, X.L., Anema, S.G., and Singh, H. (2009). Beta-lactoglobulin nanofibrils: optimising fibrillation kinetics & manipulating fibril morphology. NZ Institute of Food Science and Technology Annual Conference, Christchurch, 23-25 June. Loveday, S.M., Wang, X.L., Rao, M.A., Anema, S.G., and Singh, H. (2009). Tuning the properties of beta-lactoglobulin nanofibrils with pH, NaCl and CaCl2. 6th NIZO Dairy Conference, Papendal, The Netherlands, 30 September-2 October. 34 Riddet Institute Centre of Research Excellence Annual Report 2009 Meas, P., Paterson, A.H.J., Cleland, D.J., Bronlund, J.E., Mawson, A.J., Hardacre, A., and Rickman, J. (2009). Relating rice grain quality to conditions during sun drying. Post Harvest 2009 - Rice Exhibition and Conference (5024), Bangkok, 15 July. Meas, P., Paterson, A.H.J., Cleland, D.J., Bronlund, J.E., Mawson, A.J., Hardacre, A., and Rickman, J. (2009). Mathematical model of sun drying of rice. Post Harvest 2009 - Rice Exhibition and Conference (5024), Bangkok, 15 July. Meas, P., Paterson, A.H.J., Cleland, D.J., Bronlund, J.E., Mawson, A.J., Hardacre, A., and Rickman, J. (2009). Measurement of bed grain air conditions during sun drying of rice. Post Harvest 2009 Rice Exhibition and Conference (5024), Bangkok, 15 July. Meas, P., Paterson, A.H.J., Cleland, D.J., Bronlund, J.E., Mawson, A.J., Hardacre, A., and Rickman, J. (2009). Effects of different sun drying methods on the drying time and the rice grain quality. Post Harvest 2009 - Rice Exhibition and Conference (5024), Bangkok, 15 July. Montoya, C.A., Neufeld, K., Kish, P., and Leterme, P. (2009). Canola meals from yellow-seeded Brassica napus and B. junceae have higher digestible and net energy content in pigs than the meal from black-seeded B. napus. ADSA/CSAS/ASAS Joint Annual Meeting, Montreal, 12-16 July. Montoya, C.A., Neufeld, K., Kish, P., and Leterme, P. (2009). Effect of grinding on the digestible and net energy content of field peas (Pisum sativum) in growing pigs. ADSA/CSAS/ASAS Joint Annual Meeting, Montreal, 12-16 July. Montoya, C.A., Neufeld, K., Kish, P., and Leterme, P. (2009). Digestible and net energy content of white and regular flakes of yellow and black canola meals of Brassica napus and B. junceae in growing pigs. 2009 ADSA/ASAS Midwest Section Annual Meeting, Des Moines, Iowa, 16-18 March. Montoya, C.A., Neufeld, K., Kish, P., and Leterme, P. (2009). Evaluation of the prediction of the net energy content of canola meal and full-fat canola seeds in growing pigs. 2009 ADSA/ASAS Midwest Section Annual Meeting, Des Moines, Iowa, 16-18 March. Moongngarm, A., Bronlund, J.E., and Grigg, N. (2009). Effect of pre-processing of rice on chewing aspects and in vitro starch digestibility. 3rd Annual Biomouth Symposium, Dunedin, 10-11 June. O’Grady, G., Du, P., Egbuji, J.U., Lammers, W.J.E.P., Cheng, L.K., Pullan, A.J., and Windsor, J.A. (2009). A novel device for the minimally-invasive measurement of gastrointestinal slow wave activity. International Surgical Conference, Adelaide, Australia, September. O’Grady, G., Du, P., Egbuji, J.U., Lammers, W.J.E.P., Cheng, L.K., Pullan, A.J., and Windsor, J.A. (2009). Intra-operative high-resolution mapping of human gastric slow wave activity. International Surgical Conference, Adelaide, Australia, September. O’Grady, G., Du, P., Egbuji, J.U., Lammers, W.J.E.P., Cheng, L.K., Pullan, A.J., and Windsor, J.A. (2009). Intraoperative mapping of human gastric slow wave activity: initial results. Royal Australasian College of Surgeons’ Annual Scientific Congress, Brisbane, Australia. O’Grady, G., Du, P., Egbuji, J.U., Lammers, W.J.E.P., Cheng, L.K., Pullan, A.J., and Windsor, J.A. (2009). Intraoperative mapping of human gastric slow wave activity: initial results. New Zealand Association of General Surgeons Annual Meeting, Invercargill, New Zealand. O’Grady, G., Du, P., Egbuji, J.U, Lammers, W.J.E.P., Cheng, L.K., Pullan, A.J., and Windsor, J.A. (2009). High-resolution mapping of human gastric slow wave activity: methods and first results. Conference Proceedings for Digestive Disease Week, Chicago, USA, 30 May-4 June. O’Grady, G., Egbuji, J.U., Du, P., Lammers, W.J.E.P., Mahab, A., Cheng, L.K., Pullan, A.J., and Windsor, J.A. (2009). A novel laparoscopic device for the measurement of gastrointestinal slow wave activity and in-vivo validation. Conference Proceedings for Digestive Disease Week, Chicago, USA, 30 May-4 June. O’Grady, G., Egbuji, J.U,. Du, P., Cheng, L.K., Pullan, A.J., and Windsor, J.A. (2009). High-frequency gastric electrical stimulation for the treatment of gastroparesis: A meta-analysis. Conference Proceedings for Digestive Disease Week, Chicago, USA, 30 May-4 June. Paterson, A.H.J., Murti, R.A., Pearce, D., and Bronlund, J.E. (2009). Comparison of particle gun vs. fluid bed methods for measuring stickiness point. 4th International Symposium on Spray Dried Dairy Products, Melbourne, Australia. 15-17 April. Pleasants, A.B., Shorten, P.R., Le Roux, G., Jones, R., and Soboleva, T.K. (2009). Modelling the shelf life of fresh meat – practical experience. Invited paper. 55th International Congress of Meat Science and Technology, Meat – Muscle, Manufacturing and Meats, Denmark, August. Rao, M.A., Loveday, S.M., Creamer, L., and Singh, H. (2009). Phase behavior of high-concentration sodium caseinate dispersions derived from rheological data. The 238th American Chemical Society National Meeting, Washington DC, 16-20 August. Rao, M.A., Loveday, S.M., Creamer, L.K., and Singh, H. (2009). Rheology of nanometer- and micrometer-scale food dispersions and gels. The Institute of Food Technologists Annual Conference, Anaheim, CA, 6-9 June. Russ, A.E., Barnett, M.P.G., McNabb, W.C., Anderson, R.C., Reynolds, G.W., and Roy, N.C. (2009). Dietary ruminant milk and soy solids differentially affect body growth in the interleukin-10 gene-deficient mouse model of intestinal inflammation. 33rd Annual Scientific Meeting of Nutrition Society of Australia & Nutrition Society of New Zealand, p. 25, Newcastle, NSW, Australia, 8-11 December. Rutherfurd, S.M. and Moughan, P.J. (2009). Determining available lysine in processed feedstuffs. Proceedings of the Massey University Advancing Pork Production Seminar, pp. 7-10, Palmerston North, New Zealand. Sarkar, A., Horne, D.S., and Singh, H. (2009). Interactions of milk-protein stabilized emulsions with pancreatin and/or bile salts in an in vitro intestinal model system. 6th NIZO Dairy conference, Papendal, The Netherlands, 30 September-2 October. Shorten, P.R., Soboleva, T.K., and Pleasants, A.B. (2009). Probabilistic modelling of the growth of spoilage bacteria on chilled food products. The 3rd International Conference on Environmental, Industrial and Applied Microbiology, Lisbon, Portugal, 2-4 December. Te Morenga, L.A., Mann, J.I., Levers, M.T., Brown, R.C., and Williams, S.M. (2009). High protein diets - better than conventional low fat, high fibre diets? 33rd New Zealand Society for the Study of Diabetes Annual Scientific Meeting, Dunedin, 1-3 July. Te Morenga, L.A., Mann, J.I., Levers, M.T., Brown, R.C., and Williams, S.M. (2009). Are high carbohydrate diets the best choice for overweight women. 33rd Annual Scientific Meeting of Nutrition Society of Australia & Nutrition Society of New Zealand, Newcastle, NSW, Australia, 8-11 December. Yao, H., Foster, K., and Bronlund, J. (2009). Monitoring tongue movement during feeding using an Articulograph. 3rd Annual Biomouth Symposium, Dunedin, New Zealand, 10-11 June. CONFERENCE presentations Archer, R. (2009). Personal perspectives on getting the best from industry-university collaboration in future food research development. Riddet Institute National Conference: the Future of Food, Palmerston North, 16-18 February. Boland, M. (2009). The Riddet Institute Centre of Research Excellence. NZIFST Conference 2009 – Food Elements: Putting the pieces together, Christchurch, 23-25 June. Chung, S. (2009). The role of whey protein in body weight management. Riddet Institute National Conference: the Future of Food, Palmerston North, 16-18 February. Das, S. (2009). Research commercialisation interface – focusing on food and beverage industry. 11th Annual Food Regulation and Labelling Standards Conference, Sydney, 9-10 November. Egbuji, J.U., Du, P., O’Grady, G., Lammers, W.J., Cheng, L. Windsor, J., and Pullan, A. (2009). High-resolution mapping of gastric electrical activity. NZIFST Conference 2009 – Food Elements: Putting the pieces together, Christchurch, 23-25 June. Ferrua, M.J. (2009). Use of computational fluid dynamics to model the gastric motility during digestion. NZIFST Conference 2009 – Food Elements: Putting the pieces together, Christchurch, 23-25 June. Kaur, L. (2009). Eating kiwifruit: A key to healthy digestion. NZIFST Conference 2009 – Food Elements: Putting the pieces together, Christchurch, 23-25 June. Larsen, N. (2009). Nanotechnology and future foods. Riddet Institute National Conference: the Future of Food, Palmerston North, 16-18 February. Lentle, R. (2009). Digesta flow: the first hurdle in digestion and absorption. Riddet Institute National Conference: the Future of Food, Palmerston North, 16-18 February. Loveday, S.M. (2009). Tuning the properties of beta-lactoglobulin nanofibrils with pH, NaCl and CaCl2. 6th NIZO Dairy Conference, Papendal, The Netherlands, 1 October. McNabb, W. (2009). Molecular nutrition and gastrointestinal function. Riddet Institute National Conference: the Future of Food, Palmerston North, 16-18 February. Pullan, A. (2009). Modelling the human stomach. Riddet Institute National Conference: the Future of Food, Palmerston North, 16-18 February. Rao, M.A. (2009). Rheological characteristics of fibril gels of whey proteins. Riddet Institute National Conference: the Future of Food, Palmerston North, 16-18 February. Riou, E. (2009). Effects of added calcium on heat-induced denaturation and gelation of whey proteins. Riddet Institute National Conference: the Future of Food, Palmerston North, 16-18 February. Roy, N.C. and McNabb, W.C. (2009). Understanding the molecular interactions between our food, our microbes and us. Japan – New Zealand Functional Foods Workshop, Palmerston North, 30-31 July. Rutherfurd, S.M. (2009). Determining available lysine in processed feedstuffs. Massey University Advancing Pork Production Seminar, Palmerston North, New Zealand, 8 June. Sarkar, A. (2009). Behaviour of ß-lactoglobulin-stablized oil-in-water emulsion in a simulated gastric fluid. Riddet Institute National Conference: the Future of Food, Palmerston North, 16-18 February. Sarkar, A. (2009). In vitro evaluation of interactions of milk protein stabilized oil-in-water emulsion with artificial saliva. NZIFST Conference 2009 – Food Elements: Putting the pieces together, Christchurch, 23-25 June. Sengupta, R. (2009). Comparative molecular analyses of Lactobacillus fermentum strains affecting intestinal barrier integrity. NZIFST Conference 2009 – Food Elements: Putting the pieces together, Christchurch, 23-25 June. Singh, H. (2009). Functional foods and nanotechnology: current status and opportunities. Australian Food Science and Technology Conference, Brisbane, Australia, 13-16 July. Singh, R.P. (2009). Advances in modelling the human digestive process to develop foods for novel benefits. Riddet Institute National Conference: the Future of Food, Palmerston North, 16-18 February. Wang, X.L. (2009). Heat-induced ß-lactoglobulin fibrils – fibril formation and the effect of pH on fibril formation. Riddet Institute National Conference: the Future of Food, Palmerston North, 16-18 February. Riddet Institute Centre of Research Excellence Annual Report 2009 35 Riddet Institute at a glance The Riddet Institute is engaged in discovery-based scientific research into the structure and behaviour of complex food systems and how these interact with the consumer to influence quality of life. The Institute is a Government-funded Centre of Research Excellence The Riddet Institute is named after Professor William Riddet (1898-1958), a pioneer in food science and university education. 62 7 45 scientific staff nationwide post-graduate scholars support staff Riddet Institute income 2009 ($000) Total $5.86 million Breakdown of other income – $2,725 TEC CoRE operational – $3,135 FRST/other Govt – $857 Other income – $2,725 PBRF – $473 Trusts and Massey – $52 Industry (NZ and overseas) – $1,343 36 Riddet Institute Centre of Research Excellence Annual Report 2009 Our partners Contact Riddet Institute Headquarters – Massey University Palmerston North Cnr University Avenue and Orchard Road Massey University, Palmerston North. Private Bag 11222 Palmerston North 4442 New Zealand Phone: +64 6 350 5284 Facsimile: +64 6 350 5655 Email: [email protected] www.riddet.ac.nz
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