RNA Polymerase II Monoclonal Antibody
Sampler
Catalog Number: MPY-127R
Available Size: 3 x 0.2 mL
Description:
Clone:
A sampler pack of our three RNA Polymerase
II Monoclonal Antibodies
8WG16 (IgG2a), H5 (IgM), H14 (IgM)
Host:
Also Known As:
Mouse
DNA-directed RNA polymerase II subunit
RPB1; RNA polymerase II subunit B1; DNAdirected RNA polymerase II subunit A; RNAdirected RNA polymerase II subunit RPB1;
DNA-directed RNA polymerase III largest
subunit
Species Reactivity:
Extensive (Yeast to Human)
Form:
Ascites
HeLa cells fixed in 4% paraformaldehyde and exposed to
RNA Pol II Ab, clone H5 at a 1:1000 dilution.
Specificity:
The antibody 8WG16 was developed using RNA polymerase (RNA pol II) purified from wheat germ extract. It recognizes the C-terminal
heptapeptide repeat present on the largest subunit of pol II. Clone 8WG16 has been used to investigate the role of the C-terminal domain of the
220kD RNA pol II subunit in the formation of the transcription complex.antibody H5 was developed against a purified phosphorylated form of
RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites; ser-2 and ser-5. Both
sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. This antibody recognizes the phosphoserine 2 version of pol
II.antibody H14 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has
two physiologically important phosphorylation sites; ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal
domain. This antibody recognizes the phosphoserine 5 version of pol II.
Uses:
These antibodies are effective in immunoblotting, immunofluorescence (IF), immunoprecipitation (IP), pol II purification and functional studies.
Suggested Working Dilution:
Quality control tests were done by Western blot and Dot blot. The optimal working dilution should be determined for each specific assay
condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or
modified for resale without prior written approval.
• Western blot: 1:500*
• IF: 1:50-100
• IP: 1:100
Notes:
*Predicted MW = 220-240 kD
*use casein or BSA in the blocking solution rather than non-fat-dry milk
See reverse side for additional information
Antibody Products:
Email:
+1.800.223.0796 • +1.781.915.0990
[email protected]
www.covance.com
Page 1 of 2
RNA Polymerase II Monoclonal Antibody Sampler
Catalog Number: MPY-127R
Storage:
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing
the antibody. For long-term storage, keep the antibody at -80°C.
References:
SH Ahn, M Kim, S Buratowski. Phosphorylation of Serine 2 with the RNA Polymerase II C-Terminal Domain Couples Transcription and 3’ End
Processing. Mol. Cell 13:67-76, 2004.
JM Espinosa, RE Verdun, BM Emerson. p53 functions through stress- and promoter-specific recruitment of transcription initiation components
before and after DNA damage. Mol. Cell. 12(4):1015-27, 2003.
L Pellizzoni, B Charroux, J Rappsilber, M Mann, G Dreyfuss. A functional interaction between the survival motor neuron complex and RNA
polymerase II. J. Cell. Biol. 152(1):75-85, 2001.
SR Bhaumik, MR Green. SAGA is an essential in vivo target of the yeast acidic activator Gal4p. Genes. Dev. 15(15):1935-1945, 2001.
M Patturajan, RJ Schulte, BM Sefton, R Berezney, M Vincent, O Bensaude, SL Warren, JL Corden. Growth-related changes in phosphorylation
of yeast RNA polymerase II. J. Biol. Chem. 273(8):4689-4694, 1998.
DB Bregman, L Du, S van der Zee, SL Warren. Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete
nuclear domains. J. Cell. Biol. 129:287-298, 1995.
J Zhang, JF Corden. Identification of phosphorylation sites in the repetitive carboxyl-terminal domain of the mouse RNA polymerase II largest
subunits. J. Biol. Chem. 266:2290-2296, 1991.
NE Thompson, TH Steinberg, DB Aronson, RR Burgess. Inhibition of in-vivo and in-vitro transcription by monoclonal antibodies prepared
against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. J. Biol. Chem. 264:1151111520, 1989.
Warranty/Conditions:
Covance products may not be resold or modified for resale without prior written approval. All sales are subject to Covance Antibody Products
Terms and Conditions of Sale.
Datasheet Revision Date:
11/6/2011
Related Products:
▪ RNA Polymerase II 8WG16 Monoclonal Antibody Catalog Number MMS-126R
▪ RNA Polymerase II H14 Monoclonal Antibody Catalog Number MMS-134R
▪ RNA Polymerase II H5 Monoclonal Antibody Catalog Number MMS-129R
Page 2 of 2
Antibody Products:
Email:
+1.800.223.0796 • +1.781.915.0990
[email protected]
www.covance.com