Gene expresssion relativee to
G
GA
APDH
a2V
Hbegf
(A)
Hox10
(B)
(C)
Supplemental Figure S1. The bar diagram shows the mRNA expression of (A) a2V in
fetus; (B) Hbegf and (C) Hox10 in placenta recovered from control and PGN+poly(I:C)
treated group. Error bars=±SEM. N=6-11 each group.
Supplemental Figure 2: Uteroplacental unit of day 14.5 of pregnancy showing demarcation
of the different zones. (A) 50X, (B) 400X.
Dec
UT
Sp
Lb
PL
(A)
sp
(B)
35
iNos
1600
a
30
TNF-ɲ
1400
a
1200
25
1000
20
800
15
600
10
400
b
5
PGN+
poly(I:C)
PBS
-
ATP
-
+
+
IgG anti-a2V anti-a2V ATP
+ ATP
+
+
+
-
IgG
anti-a2V
-
-
((A))
1000
b
200
0
0
PBS
-
ATP
IgG
-
+
+
+
PGN+
poly(I:C)
anti-a2V anti-a2V ATP
+ ATP
+
+
(B)
a
900
TNF-ɲ (pg/ml)
Gen
ne expression re
elative to Gapdh
h
Supplemental Fig. 3: Anti-a2V enhances PGN+poly(I:C) induced expression of iNOS and TNF-ɲ. Expression of iNOS (A), TNF-ɲ (B) and secretion
of TNF-ɲ (C) in the RAW 264.7 macrophage cell line 2 hours after incubation with PBS or PGN+poly(I:C), followed by 3 hrs incubation with ATP,
IgG, anti-a2V or ATP+anti-a2V. P values were calculated by one way ANOVA and compare exposures with and without anti-a2V. n = 3 replicates
per condition per experiment. Depicted is a representative figure from three repeat experiments. Error bars=±SEM. aPч0.01 Significant
difference vs PGN+poly(I:C) alone or PGN+poly(I:C)+IgG and bPч0.01 Significant difference vs IgG.
800
700
600
500
400
b
300
200
100
0
PGN+
poly(I:C)
PBS
-
ATP
IgG
anti-a2V
-
+
+
+
+
(C)
anti-a2V
+ ATP
+
ATP
IgG
anti-a2V
-
-
-
-
IgG
-
anti-a2V
-
Merge
FITC
PGN+ poly(I:C)
(A)
AF-594
FITC
(B)
Merge
Control
(C)
(D)
(E)
(F)
((G))
(H)
PGN+
poly(I:C)
Supplemental Fig. 4: A representative negative control image for immunostaining. Isotype controls (A) Rabbit IgG for ȕcatenin or active capsapse-3, (B) FITC merged image with DAPI, Original magnification 200X; (C, F) Rat IgG for F4/80; (D
and G) Mouse IgG for CD11c or CD206; (E and H) FITC and AF-594 merged with DAPI in control and PGN+poly(I:C)
group, Original magnification: 400X.