Fig. S7
Genetic validation of Sec14 inhibitors. Results shown demonstrate increased sensitivity to a
Sec14 knockdown mutant (SEC14P-136) and increased resistance to Sec14 bypass mutants (cki1Δ
and kes1Δ). Growth rate relative to the no-compound control (y-axis) was measured at increasing
concentrations of 9131112, 9097855, 9053361 and 9045654 (an inactive analog) (x-axis).
Results for wild-type (CTY182; top panel), a SEC14 promoter mutant (CTY374; SEC14P-136) in
which steady-state Sec14 protein levels are reduced ~7-fold relative to wild-type (second panel),
the SEC14 bypass mutants, cki1Δ (CTY303; third panel) and kes1Δ (CTY159; bottom panel) are
shown. Data are mean +/- SEM (N = 3). IC50s, i.e. 50% inhibitory concentrations, represent the
95% confidence interval. Chemical structures are shown on the right.