FOOD
CEofix Application Note 043103
CEofixTM ANIONS 2
PN 10-004640 EuroPN 844111039
ANALYSIS OF BROMATE IN MINERAL WATER
using CEofix™ Anions2
1. Introduction
Ozonation is an alternative to chlorination for disinfection of drinking water. While source water can contain
bromide, the ozonation will tend to oxidize the bromide to bromate. Bromate has been judged by the US EPA
and WHO as a potential carcinogen.
Low level detection of bromate is therefore important for control of drinking water.
2. Method
Bromate can be detected as inorganic anion by capillary electrophoresis using inverse polarity and direct UV
detection at 200nm.
Analyses were performed with P/ACE MDQ (Beckman Coulter) using UV filter detector at 200nm, fused silica
capillary and CEofix™ Anions2 kit. We used standard method except for injection which was done electrokinetically
for 20 seconds at 3 kV.
As matrix we used a commercial available mineral water, containing following anions: Chloride 5 mg/L,
SO4 4 mg/L, NO3 1.9 mg/L and HCO3 6 mg/L.
This sample was spiked with 100 µg/L, 50 µg/L, 25 µg/L, 12.5 µg/L and 6.25 µg/L of fresh prepared BrO3.
No further sample preparation was needed.
ANALIS s.a.
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Tel: ++32 (0)81 25 50 50 • Fax: ++32 (0)81 23 12 37
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3. Results
Most anions present in mineral water such as HCO3
and SO4 are UV transparent or will absorb only at very
high concentration such as chloride. NO3 , Br (not present in the sample) and BrO3 will absorb. BrO3 is very
well separated and using electrokinetic injection low
level at the µg/L can be detected.
Linearity plot for spiked BrO3 in the analysed mineral
water gave an excellent linearity between 100 µg/L
and 5 µg/L with a R2 of 0.9985.
Results can vary from one sample matrix to another as
electrokinetic injection will give different results
depending on the concentration of anions with an high
mobility.
Figure 2 : Mineral water spiked with 100 µg/L of BrO3.
Figure 3 : Mineral water spiked with 100 µg/L of BrO3.
Detailed view.
Figure 4 : Mineral water spiked with 6.25 µg/L of BrO3.
Detailed view.
ANALIS s.a.
11 rue de Néverlée • B-5020 Suarlée • BELGIUM
Tel: ++32 (0)81 25 50 50 • Fax: ++32 (0)81 23 12 37
[email protected] • http://ceofix.analis.com