ATP Worksheet (not to be confused with a wigwam) Part A Chemical Structure of ATP ATP is a molecule that is produced & consumed in the cell. There are 3 subunits that comprise ATP; they are ribose, adenine and phosphoric acid (phosphate) Look at the following diagrams to answer these questions 1. 1. What is the molecular formula of ribose? In the term adenosine, from what molecule does the root: Adeno come ? C___ H___O___ 2. What is the ratio of H to O atoms? 3. To what organic groups does ribose belong? 4. What is the overall shape of ribose? 5. What is the molecular formula of adenine? Ose come ? 2. What atoms had to be removed to make the ATP molecule? 3. What molecule did you make with these atoms? 4. Is this an example of Hydrolysis or Condensation Reaction? (hint :the building of ATP) What atom is present in adenine that was not present in ribose? 5. Was this reaction exergonic or endergonic? ? (hint: the building of ATP) What atom is present in ribose that is not present in adenine? 6. Was this reaction catabolic or anabolic? (hint: the building of ATP) 7. What type of bonds are responsible for holding adenine, ribose & phosphoric acids together? C___H___N___ 6. 7. 8. What is the molecular formula for phosphoric acid? H___P___O___ (hint: look at the types of atoms that are involved) Part B Constructing the ATP Molecule 8. To what organic group does ATP belong? The ATP molecule is composed of 1 ribose molecule, 1 adenine molecule & 3 phosphoric acid molecules. Step 1 Cut out the Adenine and Ribose Piece and join them together. In order to get them to fit like a jigsaw puzzle, you must cut out the O-H and H from the respective pieces (cut on the dotted line). Adenosine is a combination of 2 terms, adenine and ribose. Ribose Step 2 Attach the 1 phosphoric acid (phosphate) to the adenosine, you must cut out the O-H and H from the respective pieces (cut on the dotted line). Attach the last 2 phosphoric acids to each other and the ATP molecule. Tape your ATP molecule onto a sheet of paper, you should also have 4 H2O taped down Adenine Step 3 Construct and an ADP molecule, tape it down on the flip side of your ATP paper . You should also have 3 H2O and 1 phosphoric acid (phosphate) taped down with the ADP Phosphoric Acid or Phosphate Lecture Notes Not a part of the lab Part C Converting ATP to ADP The equation is the following ATP ADP + Phosphate + Energy 1. What is the reactant? 2. What are the products? 3. Which of the products is responsible for cell being able to do work? 4. Is this equation an anabolic or catabolic reaction? 5. Is this equation an endergonic or exergonic reaction? 6. Was this equation due to condensation reaction or hydrolysis? Part D Converting ADP to ATP ATP can be formed within the mitochondrion of a cell if the reactants are available, ADP, Phosphate group and Energy ADP + Phosphate group + Energy ATP The ATP-ADP Cycle is an important continuum for all living creatures. Assign each of the vocabulary terms below to one side of the cycle 1. Why does a cell want to convert ADP to ATP? 2. What is the advantage to having a vast supply of ATP in the cell? 3. From where does the energy come to convert ADP to ATP? (hint: periods 4,5,6,7A) ATP Side 1 4. What are the reactants? 5. What is the product? 6. Is this an anabolic or catabolic reaction? 7. Is this an endergonic or exergonic reaction? 8. Was this reaction due to condensation reaction or hydrolysis? Review Analysis 1. ADP List the number AND type of sub-units needed to make: ATP ADP Side 2 Vocab Terms A. B. C. D. E. F. G. H. I. J. K. L. M. High energy molecule is being built by the mitochondrion Release of free energy so cell can do work Endergonic reaction Exergonic reaction Condensation Reaction Hydrolysis reaction Catabolic reaction Anabolic reaction Phosphorylation Eating snacks or sunlight exposure (if you are a plant) ATPase ATP Synthase De-Phosphorylation
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