CO2/O2 analysis
for every bioreactor size
From Autoclavable to Steam-In-Place, from R&D to production
A STEP AHEAD
Applikon Biotechnology
FROM AUTOCLAVABLE TO STEAM-IN-PLACE, FROM R&D TO PRODUCTION
CO2/O2 analysis for every bioreactor size
CO2
Carbon dioxide (CO2) is a product of the cellular metabolism of microorganisms. During a bioprocess,
the CO2 produced can be used to control the bioprocess or to evaluate the growth process of the culture.
The CO2 sensor can measure the (absolute) CO2 concentration of a gaseous sample.
The principle of the measurement is spectrophotometric by measuring the absorption of infrared light at two wavelengths
the percentage CO2 is measured.
The measuring range of the sensor is up to 10% CO2 (vol/vol%) within the temperature range of 15-40˚C.
Patented technology
• not sensitive for humidity
• independent from temperature
Sensor head
Detector
Light
source
Sealing
CO2
CO2
CO2
CO2
CO2
Measuring cap
Sapphire
window
CO2
Filter
CO2
CO2
CO2
CO2
CO2
Based on the infrared measuring method
working on the dual beam principle for reliable results
O2
A
O2-
O2
O2
The oxygen analysis in the exhaust gas flow of a bioreactor can be used to calculate the OUR (Oxygen Uptake Rate)
The essential part of the O2 sensor is a Zirconium Dioxide (ZrO2) element. The element is heated up to 560˚C. This type of
sensor is based on the principle of an oxygen pump cell and therefor independent of reference gasses. The sensor simply
is calibrated with ambient air.
With an oxygen pump cell, voltage and current are applied and in such a cell the limiting current is proportional to the
oxygen concentration of the sample flow.
The measuring range is: 0.1 - 25 Vol.% O2
Applikon Biotechnology
Gas
CO 2
Measuring principle
Infrared, Dual wavelength
Concentration Range
0 - 10 Vol.% CO2
Accuracy (25°C, 1,0 bar)
< 0,2% Full Scale ± 3% of value
Temperature Range
15 - 40°C, others on request
Pressure (ambient)
Compensated
Dimensions
80 x 55 mm D x H (blue cap)
Measuring unit
Material
Steel 1.4571/Sapphire/Viton
Dimensions
40 x 20 mm D x H (flow cell)
Power supply
12 V DC, 0,5 A (included)
Maintenance
Zero point once a month (ambient air)
Calibration once a year
CE proofed
Gas
O2
Measuring principle
Zirconium Dioxide
Concentration Range
0.1 - 25 Vol.% O2
Accuracy (25°C, 1,0 bar)
< 2% Full Scale ± 3% of value
Response time
2-10 sec
Temperature Range
15 - 40°C, others on request
Pressure (ambient)
Compensated
Dimensions
80 x 55 mm D x H (blue cap)
Measuring unit
Material
Steel 1.4571/Viton
Dimensions
40 x 20 mm D x H (flow cell)
Power supply
12 V DC, 1 A (included)
Maintenance
Zero point once a month (ambient air)
Calibration once a year
CE proofed
Flow-through cell for SIP reactors,
positioned after exhaust filter
(weld-ends/TC)
Flow-through cell for autoclavable
reactors, positioned after exhaust filter
(hose connection)
F R O M A U T OAC U
LA
T VOACB LL A
E VT AO BSLT EE ASM Y- ISN T- PE LMA SC E , F R O M R & D T O P R O D U C T I O N
CO2/O2 analysis for every bioreactor size
Advantages
No moving parts
Low operating costs
High accuracy
• monthly fresh air adjustment
Low maintenance
Robust
Low cross sensitivity to other gases
• assembly made of steel 1.4571 /
Independent of humidity and pressure
sapphire / Viton
Calibration with ambient air
• compact construction
Easy to use
Durable
Economically priced
Long term reliability (< 2% reading / year)
Low investment costs
• no additional exhaust gas cooling
• no extra gas lines required
• quick mounting of standard connections
Ordering information
Z073101010
• CO2 gas sensor, 0-10%, 6mm HC*
Z015002510
• O2 gas sensor, 0-25%, 6mm HC*
* HC = Hose barbed Connection. Weld-end or TC to be specified upon order.
A STEP AHEAD
Applikon Biotechnology BV De Brauwweg 13, P.O. Box 149, 3100 AC Schiedam, The Netherlands.
Phone: +31 10 298 35 55. Fax: +31 10 437 96 48. E-mail: [email protected]
www.applikon.com