Conventional Restriction Enzymes Product Description Size Price(Rs.) FERMENTAS is a world leader in the discovery, manufacturing and marketing of quality molecular biologicals and providing services to the international research community. As a dynamically evolving biotech company, they strive to make vital contributions to the rapidly growing fields of genomics, proteomics and microfluidics through key partnerships and collaborations. Their solid scientific and R&D background, product development experience, flexibility and strong team spirit make us an ideal research and development partner to bring new technologies to the market. CAT# Size Price(Rs.) ER1841 Product Description BauI 200 units 4,555 ER0721 BclI 1000 units 1,335 ER0725 BclI 3000 units 3,613 ER0722 BclI 5000 units 5,341 ER0061 BcnI 1000 units 2,199 ER0062 BcnI 5000 units 8,797 ER1251 BcuI 400 units 3,691 ER1252 BcuI 2000 units 14,766 3,927 ER1961 BdaI 50 units ER1591 BfiI 50 units 5,027 ER1592 BfiI 250 units 20,107 ER1161 BfmI 200 units 4,006 ER1162 BfmI 1000 units 16,023 ER1501 BfuI 100 units 2,199 ER1502 BfuI 500 units 8,797 ER0071 BglI 2000 units 3,220 RESTRICTION ENZYMES ER0072 BglI 5x2000 units 12,881 A ER0081 BglII 500 units 1,335 5,891 ER0082 BglII 2500 units 5,341 5,027 ER1421 Bme1390I 500 units 2,513 ER1422 Bme1390I 2500 units 10,053 ER1431 BoxI 500 units 2,513 ER1011 BpiI 200 units 2,828 ER1012 BpiI 1000 units 11,310 ER1311 BplI 100 units 4,241 ER1312 BplI 500 units 16,965 ER1181 Bpu10I 200 units 6,205 ER1182 Bpu10I 1000 units 24,819 ER0091 Bpu1102I 200 units 2,121 ER0092 Bpu1102I 1000 units 8,483 ER1081 BseDI 300 units 3,456 ER1082 BseDI 1500 units 13,823 ER2061 ER1581 AanI (PsiI) NEW AarI 200 units 25 units ER1582 AarI 125 units 20,107 ER1721 AasI 300 units 4,084 ER1722 AasI 1500 units 16,337 ER0991 AatII 300 units 2,121 ER0992 AatII 1500 units 8,483 ER0901 Acc65I 1000 units 2,121 ER0902 Acc65I 5000 units 8,483 ER1231 AdeI 500 units 2,828 ER1232 AdeI 2500 units 11,310 ER1941 AjiI 200 units 4,398 ER1951 AjuI 100 units 3,927 ER1801 AlfI 50 units 7,226 ER0871 BseGI 500 units 2,435 ER1491 AloI 100 units 8,247 ER0872 BseGI 2500 units 9,739 ER1492 AloI 500 units 32,988 ER1711 BseJI 2000 units 3,377 ER0011 AluI 600 units 3,142 ER1201 BseLI 500 units 2,749 ER0012 AluI 3000 units 12,567 ER1202 BseLI 2500 units 10,996 ER0021 Alw21I 500 units 2,513 ER1261 BseMI 100 units 3,377 ER0022 Alw21I 2500 units 10,053 ER1262 BseMI 500 units 13,509 ER0031 Alw26I 1000 units 3,142 ER1401 BseMII 100 units 4,713 ER0032 Alw26I 5000 units 12,567 ER1402 BseMII 500 units 18,850 ER0041 Alw44I 1000 units 2,278 ER0881 BseNI 1000 units 4,713 BseNI 5000 units 18,850 ER0042 Alw44I 5000 units 9,111 ER0882 ER1411 ApaI 3000 units 2,435 ER1441 BseSI 500 units 5,184 ER1415 ApaI 5000 units 3,927 ER1442 BseSI 2500 units 20,735 ER1412 ApaI 5x3000 units 9,739 ER1451 BseXI 100 units 3,299 ER1452 BseXI 500 units 13,195 ER0921 Bsh1236I 500 units 2,199 ER0922 Bsh1236I 2500 units 8,797 ER0891 Bsh1285I 600 units 3,142 ER1001 BshNI 2000 units 1,964 ER1461 BshTI 200 units 3,849 B 2008-09 Chemicals, Reagents & Kits CAT# ER0051 ER0055 ER0052 ER0053 116 BamHI BamHI BamHI BamHI, HC Call +91-11-45027000 4000 units 10000 units 5x4000 units 20000 units 1,335 3,142 5,341 5,341 Fax. +91-11-25419631 E-mail : [email protected] Size Price(Rs.) CAT# Product Description Size Price(Rs.) CAT# Product Description ER1462 BshTI 1000 units Size Price(Rs.) 15,394 CAT# Product Description Size Price(Rs.) ER0121 Bsp119I 2500 units 2,513 ER0131 Bsp120I 1500 units 1,571 ER0132 Bsp120I 5x1500 units 6,283 ER0133 Bsp120I, HC 7500 units 6,283 ER0931 Bsp1407I 300 units 1,728 ER0932 Bsp1407I 1500 units 6,912 ER0781 Bsp143I 300 units 3,142 ER0782 Bsp143I 1500 units 12,567 ER0791 Bsp143II 1000 units 2,121 Bsp143II 5000 units 8,483 ER0111 Bsp68I 800 units 3,220 ER0112 Bsp68I 4000 units 12,881 ER1151 BspLI 200 units 3,142 ER1152 BspLI 1000 units 12,567 ER2041 BspoI(BmtI) 200 units 4,713 ER2042 BspoI(BmtI) 1000 units 18,850 ER1321 BspPI 100 units 2,199 ER1322 BspPI 500 units 8,797 ER0831 BspTI 1000 units 2,121 Fax. +91-11-25419631 Call +91-11-45027000 117 2008-09 E-mail : [email protected] ER0792 Chemicals, Reagents & Kits Product Description Conventional Restriction Enzymes CAT# Conventional Restriction Enzymes Product Description ER0832 BspTI Size Price(Rs.) 5000 units ER0701 Size Price(Rs.) 8,483 ER0281 Eco24I 1500 units 4,006 Bst1107I 500 units 2,435 ER0291 Eco31I 1000 units 4,713 ER0702 Bst1107I 2500 units 9,739 ER0292 Eco31I 5000 units 18,850 ER1021 BstXI 500 units 1,964 ER0301 Eco32I 2000 units 2,121 ER1022 BstXI 2500 units 7,854 ER0305 Eco32I 4000 units 3,927 ER0141 Bsu15I 600 units 2,121 ER0302 Eco32I 5x2000 units 8,483 ER0145 Bsu15I 1000 units 3,534 ER0303 Eco32I, HC 10000 units 8,483 ER0142 Bsu15I 3000 units 8,483 ER0311 Eco47I 800 units 1,806 ER0151 BsuRI 3000 units 1,649 ER0312 Eco47I 4000 units 7,226 ER0152 BsuRI 5x3000 units 6,598 ER0321 Eco47III 200 units 6,833 ER1741 BveI 250 units 4,398 ER0322 Eco47III 1000 units 27,333 ER0331 Eco52I 500 units 4,163 ER0332 Eco52I 2500 units 16,651 C CAT# Product Description ER1391 CaiI 500 units 3,849 ER0341 Eco57I 200 units 4,713 ER1392 CaiI 2500 units 15,394 ER0342 Eco57I 1000 units 18,850 ER0181 Cfr10I 200 units 2,356 ER1671 Eco57MI 50 units 6,205 ER0182 Cfr10I 1000 units 9,425 ER0361 Eco72I 2000 units 2,435 ER0191 Cfr13I 1000 units 3,142 ER0362 Eco72I 5x2000 units 9,739 ER0192 Cfr13I 5000 units 12,567 ER0371 Eco81I 500 units 2,435 ER0201 Cfr42I 1200 units 2,121 ER0372 Eco81I 2500 units 9,739 ER0205 Cfr42I 2000 units 3,613 ER0381 Eco88I 1000 units 2,121 ER0202 Cfr42I 5x1200 units 8,483 ER0391 Eco91I 1000 units 1,806 ER0171 Cfr9I 300 units 4,163 ER0392 Eco91I 5000 units 7,226 ER0172 Cfr9I 1500 units 16,651 ER0261 EcoO109I 2000 units 3,142 ER0161 CfrI 200 units 4,006 ER0271 EcoRI 5000 units 1,414 ER0162 CfrI 1000 units 16,023 ER0275 EcoRI 10000 units 2,828 EcoRI 5x5000 units 4,870 25000 units 4,870 ER0741 CpoI 200 units 5,027 ER0272 ER0742 CpoI 1000 units 20,107 ER0273 EcoRI, HC ER1901 CseI 100 units 4,713 ER1921 EcoRII 200 units 7,776 EcoRII 1000 units 31,103 500 units 6,048 ER1902 CseI 500 units 18,850 ER1922 ER0211 Csp6I 1500 units 3,613 ER0441 EheI ER0451 Esp3I 200 units 4,555 ER0452 Esp3I 1000 units 18,222 4,948 D ER1701 DpnI 500 units 2,356 ER1705 DpnI 1000 units 4,320 ER1702 DpnI 2500 units 9,425 ER1811 FaqI 100 units ER0221 DraI 1500 units 1,964 ER1661 FspAI 100 units 6,205 5x1500 units 7,854 ER1662 FspAI 500 units 24,819 7500 units 7,854 ER1761 FspBI 500 units 5,184 ER1762 FspBI 2500 units 20,449 ER0461 GsuI 100 units 6,205 ER0462 GsuI 500 units 24,819 ER1851 HhaI 2000 units 3,691 ER0471 Hin1I 300 units 1,335 ER0472 Hin1I 1500 units 5,341 ER1831 Hin1II 300 units 3,299 ER1601 Hin4I 50 units 4,948 ER1602 Hin4I 250 units 19,793 ER0222 DraI ER0223 DraI, HC E ER0231 Eam1104I 300 units 2,121 ER0232 Eam1104I 1500 units 8,483 ER0241 2008-09 Chemicals, Reagents & Kits CAT# Eam1105I 1000 units 4,870 ER0242 Eam1105I 5000 units 19,478 ER0251 Ecl136II 1500 units 3,299 ER0401 Eco105I 600 units 5,027 ER0402 Eco105I 3000 units 20,107 ER0411 Eco130I 2500 units 2,749 ER0421 ER0422 118 Eco147I Eco147I Call +91-11-45027000 1000 units 5000 units 2,670 10,682 F G H Fax. +91-11-25419631 E-mail : [email protected] Hin6I 2000 units 3,299 ER0731 Mph1103I 1000 units 3,456 ER0482 Hin6I 5x2000 units 13,195 ER0732 Mph1103I 5000 units 13,823 ER0491 HincII 500 units 2,356 ER2021 MreI (Sse 2321) 300 units 4,477 ER0492 HincII 2500 units 9,425 ER2022 MreI (Sse 2321) 1500 units 17,908 ER0501 HindIII 5000 units 1,414 ER0541 MspI 3000 units 2,513 ER0505 HindIII 10000 units 2,828 ER0542 MspI 5x3000 units 10,053 ER0502 HindIII 5x5000 units 4,870 ER1341 MssI 250 units 2,749 ER0503 HindIII, HC 25000 units 4,870 ER1342 MssI 1250 units 10,996 ER0801 HinfI 2000 units 2,042 ER0751 MunI 300 units 2,749 ER0802 HinfI 5x2000 units 8,168 ER0752 MunI 1500 units 10,996 ER0803 HinfI, HC 10000 units 8,168 ER0961 Mva1269I 200 units 2,278 1,492 ER0962 Mva1269I 1000 units 7,854 5000 units 5,969 ER0551 MvaI 2000 units 2,042 ER0552 MvaI 5x2000 units 8,168 4,084 ER0511 HpaII Size 1000 units Price(Rs.) CAT# Product Description Size Price(Rs.) ER0512 HpaII ER1101 HphI 300 units 3,299 ER1102 HphI 1500 units 13,195 ER1571 Hpy8I 200 units 4,006 ER1572 Hpy8I 1000 units 16,023 ER0571 NcoI 500 units ER1731 HpyF10VI 300 units 3,927 ER0575 NcoI 1000 units 7,069 ER0572 NcoI 2500 units 16,337 ER0581 NdeI 500 units 2,042 ER0582 NdeI 2500 units 8,168 ER0585 NdeI 4000 units 10,996 ER0971 NheI 500 units 2,749 ER0975 NheI 1000 units 4,713 ER0972 NheI 2500 units 10,996 ER1511 NmuCI 200 units 4,713 ER1512 NmuCI 1000 units 18,850 ER0591 NotI 300 units 3,063 ER0595 NotI 500 units 4,713 ER0592 NotI 1500 units 12,253 ER0593 NotI, HC 1500 units 12,253 ER1221 NsbI 400 units 3,456 ER1222 NsbI 2000 units 13,823 ER1732 HpyF10VI ER1881 HpyF3I ER1882 HpyF3I 1500 units 15,708 500 units 3,770 2500 units 15,080 K ER0531 Kpn2I 500 units 2,278 ER0532 Kpn2I 2500 units 9,111 ER0521 KpnI 4000 units 3,456 ER0522 KpnI 5x4000 units 13,823 ER0523 KpnI, HC 20000 units 13,823 ER1031 KspAI 500 units 1,954 ER1032 KspAI 2500 units 7,854 L ER1931 LguI 100 units 5,105 ER1932 LguI 500 units 20,425 ER2071 Lsp1109I (BbvI) 1x200 units 3,927 ER1621 LweI 100 units 4,948 ER1622 NEW LweI 500 units 19,793 N O ER1631 OliI 200 units 3,770 ER1632 OliI 1000 units 15,080 P M ER2081 MauBI NEW 1x100 units 3,927 ER1271 MbiI 1000 units 3,063 ER0811 MboI 300 units 3,142 ER0812 MboI 1500 units 12,567 ER0821 MboII 300 units 3,456 ER0822 MboII 1500 units 13,823 ER1211 MlsI 200 units 4,006 ER1212 MlsI 1000 units 16,023 MluI 1000 units 2,749 ER0562 MluI 5000 units 10,996 ER1071 ER1072 MnlI MnlI E-mail : [email protected] 300 units 1500 units 3,142 12,567 PaeI 500 units 6,519 ER0602 PaeI 2500 units 26,076 ER1281 PagI 400 units 3,142 ER1282 PagI 2000 units 12,567 ER1861 PasI 200 units 7,147 ER1091 PauI 200 units 3,142 ER1092 PauI 1000 units 12,567 ER1521 PdiI 200 units 1,728 ER1522 PdiI 1000 units 6,912 ER1531 PdmI 500 units 2,356 ER1532 PdmI 2500 units 9,425 ER1781 PfeI 500 units 4,791 Fax. +91-11-25419631 Call +91-11-45027000 119 2008-09 ER0561 ER0601 Chemicals, Reagents & Kits Product Description ER0481 Conventional Restriction Enzymes CAT# Conventional Restriction Enzymes Product Description Size Price(Rs.) ER0851 Pfl23II 300 units 4,555 ER1892 SgsI 1500 units 11,310 ER1751 PfoI 200 units 9,181 ER0661 SmaI 1200 units 2,356 ER1541 PpiI 50 units 4,161 ER0665 SmaI 2000 units 3,927 ER1542 PpiI 250 units 16,651 ER0662 SmaI 5x1200 units 9,425 ER1971 Ppu21I 500 units 4,398 ER0663 SmaI, HC 6000 units 9,425 ER1871 PscI 200 units 5,498 ER1241 SmiI 1000 units 2,749 ER1872 PscI 1000 units 21,992 ER1242 SmiI 5000 units 10,996 ER0941 Psp1406I 300 units 4,713 ER1981 SmoI 200 units 4,398 ER0942 Psp1406I 1500 units 18,850 ER1691 SmuI 50 units 4,320 ER0761 Psp5II 500 units 4,398 ER1692 SmuI 250 units 17,279 ER0611 PstI 3000 units 1,492 ER1791 SsiI 200 units 4,398 ER0615 PstI 10000 units 4,634 ER0771 SspI 500 units 2,513 ER0612 PstI 5x3000 units 5,969 ER0772 SspI 2500 units 10,053 ER0613 PstI, HC 15000 units 5,969 ER1551 PsuI 500 units 2,513 ER1331 PsyI 1000 units 3,927 ER1361 TaaI 200 units 3,691 ER0621 PvuI 300 units 6,519 ER1362 TaaI 1000 units 14,766 ER0622 PvuI 1500 units 26,076 ER1141 Size Price(Rs.) CAT# Product Description TaiI 400 units 3,927 TaiI 2000 units 15,708 1,964 T ER0631 PvuII 2500 units 1,885 ER1142 ER0635 PvuII 5000 units 3,770 ER0671 TaqI 3000 units ER0632 PvuII 5x2500 units 7,540 ER0672 TaqI 5x3000 units 7,854 7,854 ER0673 TaqI, HC 15000 units 7,854 ER1351 TasI 1000 units 3,534 ER1352 TasI 5000 units 14,138 3,299 ER1291 TatI 100 units 4,163 TatI 500 units 16,651 ER0633 PvuII, HC 12500 units R ER1121 RsaI 1000 units ER1122 RsaI 5000 units 13,195 ER1292 ER2001 RseI 200 units 4,477 ER1651 TauI 50 units 4,948 ER2002 RseI 1000 units 17,908 ER1652 TauI 250 units 19,793 ER0981 Tru1I 300 units 2,749 S 2008-09 Chemicals, Reagents & Kits CAT# ER0982 Tru1I 1500 units 10,996 ER1131 SacI 1200 units 2,356 ER0983 Tru1I, HC 1500 units 10,996 ER1135 SacI 2000 units 3,927 ER2101 TscAI (TspRI) 1x1000 units 3,927 ER1132 SacI 5x1200 units 9,425 ER1991 TsoI 50 units 3,927 ER1133 SacI, HC 6000 units 9,425 ER1911 TstI 100 units 3,927 ER0641 SalI 1500 units 2,356 ER0645 SalI 2000 units 3,927 ER0642 SalI 5x1500 units 9,425 ER0711 Van91I 400 units 1,885 ER0643 SalI, HC 7500 units 9,425 ER0712 Van91I 2000 units 7,540 ER1641 SatI 200 units 4,634 ER0911 VspI 1000 units 2,356 18,536 ER0912 VspI 5000 units 9,425 NEW V ER1642 SatI 1000 units ER0431 ScaI 1000 units 2,513 ER0432 ScaI 5000 units 10,053 ER1371 SchI 1000 units 5,105 ER1301 XagI 1000 units 3,613 XagI 5000 units 14,452 X ER1191 SdaI 300 units 4,471 ER1302 ER1192 SdaI 1500 units 17,908 ER1381 XapI 500 units 2,828 XapI 2500 units 11,310 1500 units 1,728 ER0651 SduI ER2091 SfaAI (AsiSI) ER1821 SfiI ER2031 SgrDI ER1891 120 NEW SgsI Call +91-11-45027000 500 units 3,142 ER1382 1x1000 units 3,927 ER0681 XbaI 1000 units 2,592 ER0685 XbaI 3000 units 3,456 200 units 4,320 ER0682 XbaI 5x1500 units 6,912 2,828 ER0683 XbaI, HC 7500 units 6,912 ER1471 XceI 200 units 3,770 300 units Fax. +91-11-25419631 E-mail : [email protected] Product Description Size Price(Rs.) CAT# Product Description Size Chemicals, Reagents & Kits CAT# Price(Rs.) 2008-09 E-mail : [email protected] Fax. +91-11-25419631 Call +91-11-45027000 121 Chemicals, Reagents & Kits Product Description Size Price(Rs.) CAT# Product Description Size Price(Rs.) 2008-09 CAT# 122 Call +91-11-45027000 Fax. +91-11-25419631 E-mail : [email protected] Product Description Size Price(Rs.) CAT# Product Description Size Chemicals, Reagents & Kits CAT# Price(Rs.) 2008-09 E-mail : [email protected] Fax. +91-11-25419631 Call +91-11-45027000 123 Chemicals, Reagents & Kits Product Description Size Price(Rs.) CAT# Product Description Size Price(Rs.) 2008-09 CAT# 124 Call +91-11-45027000 Fax. +91-11-25419631 E-mail : [email protected] XceI 1000 units Size Price(Rs.) 15,089 FD2094 ER0691 XhoI 2000 units 1,571 FD0384 FastDigest® AsiSI (SfaAI) 100 Reaction NEW 4,713 FastDigest® AvaI (Eco88I) 200 react 5,616 ER0695 XhoI 5000 units 3,927 FD0314 FastDigest® AvaII (Eco47I) 200 react 5,616 ER0692 XhoI 5x2000 units 6,283 FD1564 FastDigest® AvrII (XmaJI) 20 react 10,368 ER0693 XhoI, HC 10000 units 6,283 FD0054 FastDigest® BamHI 800 react 3,456 ER1561 XmaJI 200 units 7,776 FD1004 FastDigest® BanI (BshNI) 300 react 3,887 ER1562 XmaJI 1000 units 31,103 FD1014 FastDigest® BbsI (BpiI) 20 react 3,887 ER1481 XmiI 400 units 3,770 FD2074 FastDigest® BbvI (Lsp1109I) ER1482 XmiI 2000 units 15,080 FD0724 FastDigest® BclI FD1764 FastDigest® BfaI (FspBI) 50 react 6,911 FD0074 FastDigest® BglI 200 react 4,752 Nicking Enzyme CAT# Product Description Size Price(Rs.) 100 React NEW 4,713 300 react 5,616 ER1681 Nb.Bpu10I 1000 units 5,419 FD0083 FastDigest® BglII 100 react 3,456 ER2011 Nt.Bpu10I 1000 units 4,320 FD0084 FastDigest® BglII 200 react 6,480 ER2051 Nb.Mva 12691( Nb.Bsml) 1000 units 4,320 FD0094 FastDigest® BlpI (Bpu1102I) 50 react 6,911 FD1444 FastDigest® Bme1580I (BseSI) 50 react 6,480 FD2044 FastDigest® BmtI( BspOI) 5,655 FD1974 FastDigest® BsaAI(Ppu21I) FD0474 FastDigest® BsaHI (Hin1I) FD1084 FastDigest® BsaJI (BseDI) 20 react 3,190 FD0874 FastDigest® BseGI 100 react 6,048 FD0884 FastDigest® BseNI 100 react 6,048 FD0924 FastDigest® Bsh1236I 100 react 5,616 FD1204 FastDigest® BslI (BseLI) 100 react 6,911 FD0454 FastDigest® BsmBI (Esp3I) FD0124 FastDigest® Bsp119I 200 react 3,024 FD0134 FastDigest® Bsp120I 200 react 3,456 FD0654 FastDigest® Bsp1286I (SduI) FD0933 FastDigest® Bsp1407I 50 react 3,456 FD0934 FastDigest® Bsp1407I 100 react 6,480 FD1284 FastDigest® BspHI (PagI) NEW 50 react 4,575 FD1274 FastDigest® BsrBI (MbiI) NEW 100 react 3,927 FD0184 FastDigest® BsrFI (Cfr10I) 50 react 6,480 FD1024 FastDigest® BstXI 100 react 5,184 FD0704 FastDigest® BstZ17I (Bst1107I) 100 react 6,480 Homing Enzyme ER1771 I-SceI 250 units 4,447 FastDigest® RESTRICTION ENZYMES ( Digestion in 5 minutes) 20 react 50 react NEW 4,880 100 react 5,184 20 react NEW 5,337 50 react NEW 3,812 50 react NEW 3,050 FD0374 FastDigest® Bsu36I (Eco81I) 100 react 3,024 FD0143 FastDigest® ClaI (Bsu15I) 50 react 2,592 FastDigest® AccI (XmiI) 50 react 6,480 FD0144 FastDigest® ClaI (Bsu15I) 100 react 4,752 FD1794 FastDigest® AciI (SsiI) 20 react 6,048 FD0214 FastDigest® Csp6I 100 react 3,456 FD0944 FastDigest® AclI (Psp1406I) 20 react 3,887 FD1884 FastDigest® DdeI (HpyF3I) 50 react 5,011 FD0834 FastDigest® AfIII (BspTI) NEW 150 react 3,927 FD1703 FastDigest® DpnI 50 react 3,024 FD0324 FastDigest® AfeI (Eco47III) 20 react 9,504 FD1704 FastDigest® DpnI 100 react 6,048 FD1464 FastDigest® AgeI (BshTI) 20 react 5,184 FD0224 FastDigest® DraI 200 react 3,456 FD1954 FastDigest® AjuI 20 react 4,870 FD1234 FastDigest® DraIII (AdeI) 50 react 3,887 FD1634 FastDigest® AleI (Olil) 20 react 4,791 FD1724 FastDigest® DrdI (AasI) FD0024 FastDigest® Alw21I 100 react 6,048 FD0334 FastDigest® EagI (Eco52I) FD0034 FastDigest® Alw26I 100 react 4,320 25 react NEW 3,887 50 react NEW 5,419 FD0234 FastDigest® EarI (Eam1104I) 50 react 5,184 FD0244 FastDigest® Eam1105I 100 react 6,480 300 react FD0904 FastDigest® Acc65I FD1484 ® NEW 50 react NEW 3,988 FastDigest AlwNI (CaiI) FD1414 FastDigest® ApaI 3,456 FD0254 FastDigest® Ecl136II 100 react 3,456 FD0044 FastDigest® ApaLI (Alw44I) 200 React NEW 4,084 FD0293 FastDigest® Eco31I 50 react 3,456 FD1894 FastDigest® AscI (SgsI) 100 react 6,048 FD0294 FastDigest® Eco31I 100 react 6,480 FD0914 FastDigest® AseI (VspI) 200 react 6,048 E-mail : [email protected] Fax. +91-11-25419631 Call +91-11-45027000 125 2008-09 FD1394 Chemicals, Reagents & Kits Product Description ER1472 FastDigestTM Restriction Enzymes CAT# Product Description Size Price(Rs.) CAT# Product Description Size Price(Rs.) CAT# Product Description Size Price(Rs.) CAT# Product Description FD0394 FastDigest® Eco91I 200 react 4,752 FD0494 FastDigest® HincII Size Price(Rs.) 100 react FD1304 FastDigest® EcoNI (XagI) 100 react 5,184 FD0504 FastDigest® HindIII 800 react 5,616 3,456 FD0264 FastDigest® EcoO109I NEW 200 react FastDigest® EcoRI 800 react 4,163 FD0804 FastDigest® HinfI 400 react 5,184 FD0274 3,456 FD0484 FastDigest® HinP1I (Hin6I) 200 react 4,752 FD0303 FastDigest® EcoRV (Eco32I) 200 react 3,456 FD1034 FastDigest® HpaI (KspAI) 50 react 3,024 FD0304 FastDigest® EcoRV (Eco32I) 400 react 8,048 FD0514 FastDigest® HpaII 200 react 3,887 FD0443 FastDigest® EheI 20 react 3,456 FD1574 FastDigest® Hpy8I 20 react 5,616 FD0444 FastDigest® EheI 50 react 7,776 FD1734 FastDigest® HpyF10VI 20 react 3,456 FastDigest® Kpn2I 100 react 5,616 4,320 FastDigestTM Restriction Enzymes 2008-09 Chemicals, Reagents & Kits CAT# FD1644 FastDigest® Fnu4HI (SatI) 20 react 5,616 FD0534 FD1664 FastDigest® FspAI 20 react 8,207 FD0524 FastDigest® KpnI 300 react FD1224 FastDigest® FspI (NsbI) 50 react 5,616 FD0814 FastDigest® MboI 50 react 6,911 FD0154 FastDigest® HaeIII (BsuRI) 400 react 3,456 FD0824 FastDigest® MboII 50 react 4,752 FD1854 FastDigest® HhaI 200 react 5,184 FD0753 FastDigest® MfeI (MunI) 20 react 2,952 126 Call +91-11-45027000 Fax. +91-11-25419631 E-mail : [email protected] Size Size Price(Rs.) FastDigest® MfeI (MunI) 50 react Price(Rs.) 5,184 CAT# FD0663 Product Description FastDigest® SmaI 100 react 2,592 FD0564 FastDigest® MluI 100 react 3,887 FD0664 FastDigest® SmaI 200 react 5,184 FD1374 FastDigest® MlyI (SchI) NEW 100 react 6,126 FD0404 FastDigest® SnaBI (Eco105I) 50 react 5,788 FD1074 FastDigest® MnlI 50 react 6,911 FD1253 FastDigest® SpeI (BcuI) 20 react 2,592 FD2024 FastDigest® MreI 20 react 3,927 FD1254 FastDigest® SpeI (BcuI) 50 react 6,048 FD1214 FastDigest® MscI (MlsI) 50 react 6,911 FD0604 FastDigest® SphI (PaeI) 20 react 3,456 FD2004 FastDigest® MslI (RseI) 20 react 5,875 FD0774 FastDigest® SspI 100 react 6,480 FD0544 FastDigest® MspI 400 react 4,320 FD0424 FastDigest® StuI (Eco147I) 100 react 3,456 FD1344 FastDigest® MssI 100 react 6,911 FD0414 FastDigest® StyI (Eco130I) 200 react 3,456 FD0964 FastDigest® Mva1269I 50 react 6,911 FD1244 FastDigest® SwaI (SmiI) 200 react 6,911 FD0554 FastDigest® MvaI 200 react 3,456 FD1364 FastDigest® TaaI 20 react 4,752 FD1524 FastDigest® NaeI (PdiI) NEW 50 react 4,422 FD1144 FastDigest® TaiI 50 react 6,048 FD0064 FastDigest® NciI (BcnI) 5,616 FD0674 FastDigest® TaqI 400 react 4,320 FD0573 FastDigest® NcoI 20 react 2,592 FD0984 FastDigest® Tru1I 50 react 6,048 FD0574 FastDigest® NcoI 100 react 10,368 FD1354 FastDigest® Tsp509I (TasI) 100 react NEW 4,634 FD0583 FastDigest® NdeI 100 react 3,887 FD2104 FastDigest® TspRI (TscAI) 100 React NEW 4,713 FD0584 FastDigest® NdeI 300 react 11,231 FD1383 FastDigest® XapI 50 react 3,887 FD0973 FastDigest® NheI NEW 50 React 3,299 FD1384 FastDigest® XapI 100 react 7,344 FD0974 FastDigest® NheI NEW 100 React 5,969 FD0684 FastDigest® XbaI 300 react 3,887 FD1834 FastDigest® NlaIII (Hin1II) 100 react NEW 5,837 FD0694 FastDigest® XhoI 400 react 3,456 200 react FD1154 FastDigest® NlaIV (BspLI) 20 react 4,320 FD1514 FastDigest® NmuCI 20 react 6,048 ADDITIONAL BUFFERS FOR RE’S FD0593 FastDigest® NotI 20 react 2,592 BB5 10X Buffer B 5x1 ml 1,021 FD0594 FastDigest® NotI 50 react 6,480 BG5 10X Buffer G 5x1 ml 1,021 4,752 BO5 10X Buffer O 5x1 ml 1,021 BR5 10X Buffer R 5x1 ml 1,021 BY5 10X Buffer Tango™ 5x1 ml 1,021 B30 Buffer Set for Restriction Endonucleases 5x1 ml 1,021 FD0734 FD1474 FastDigest® NsiI (Mph1103I) 100 react 4,713 FD1534 FastDigest® NspI (XceI) NEW 50 react FastDigest® PdmI 100 react FD0714 FastDigest® PflMI (Van91I) 100 react 6,048 FD1754 FastDigest® PfoI 20 react 12,096 FD0364 FastDigest® PmlI (Eco72I) 20 react NEW 2,900 UNIQUE BUFFERS FOR RE’S FD0764 FastDigest® PpuMI (Psp5II) 50 react 6,048 B27 FD1434 FastDigest® PshAI (BoxI) 100 react 6,911 FD2064 FastDigest® PsiI (AanI) NEW 20 React 7,069 B57 FD0614 FastDigest® PstI 800 react 4,752 B59 FD1334 FastDigest® PsyI 100 react 4,634 B31 FD1554 FastDigest® PsuI 150 React 4,320 B13 FD0624 FastDigest® PvuI 20 react 6,048 B04 FD0634 FastDigest® PvuII 400 react 4,320 B02 FD1124 FastDigest® RsaI 100 react 4,320 B25 FD1133 FastDigest® SacI 100 react 3,024 FD1134 FastDigest® SacI 200 react 5,184 FD0644 FastDigest® SalI 200 react 4,320 FD1934 FastDigest® SapI (LguI) 20 react 6,911 FD0194 FastDigest® Sau96I (Cfr13I) 100 react 4,320 FD1194 FastDigest® SbfI (SdaI) 20 react 4,320 FD0434 FastDigest® ScaI 100 react 3,159 FD1424 FastDigest® ScrFI (Bme1390I)100 react 6,480 FD1164 FastDigest® SfcI (BfmI) 5,184 10X Buffer AarI, AjiI, Bpu10I, ScaI, PasI 1 ml 1,021 10X Buffer BamHI 5x1 ml 1,021 10X Buffer BfuI 1 ml 1,021 10X Buffer BseXI 1 ml 1,021 10X Buffer Bsp143I 1 ml 1,021 10X Buffer Cfr10I 1 ml 1,021 10X Buffer Cfr9I 1 ml 1,023 10X Buffer Eam1105I 1 ml 1,021 B26 10X Buffer Ecl136II, SacI 1 ml 1,021 B22 10X Buffer Eco52I 1 ml 1,021 B12 10X Buffer EcoRI 5x1 ml 1,021 B29 10X Buffer KpnI 1 ml 1,021 B24 10X Buffer SdaI 1 ml 1,021 B23 10X Buffer SduI, Ppu21I 1 ml 1,021 B28 10X Buffer TaqI 1 ml 1,021 5x1 ml 1,021 B19 Fax. +91-11-25419631 Dilution Buffer for Restriction Endonucleases Call +91-11-45027000 127 2008-09 E-mail : [email protected] 20 react 6,048 Chemicals, Reagents & Kits Product Description FD0754 FastDigestTM Restriction Enzymes CAT# DNA/RNA Modifying Enzymes Product Description Size Price(Rs.) DNA/RNA MODIFYING ENZYMES CAT# EP0162 Mesophilic DNA Polymerases EP0041 DNA Polymerase I, E.coli 500 units 3,142 EP0042 DNA Polymerase I, E.coli 2500 units 12,567 EP0051 Klenow Fragment 300 units 3,927 EP0052 Klenow Fragment 1500 units 15,708 EP0054 Klenow Fragment, LC 300 units 3,927 EP0421 Klenow Fragment, exo– 300 units 3,927 EP0422 Klenow Fragment, exo– 1500 units 15,708 EP0091 phi29 DNA Polymerase 250 units 4,320 EP0092 phi29 DNA Polymerase 1000 units 14,138 EP0094 phi29 DNA Polymerase 5000 units 55,500 EP0061 T4 DNA Polymerase 100 units 2,513 EP0062 T4 DNA Polymerase 500 units 10,053 EP0081 T7 DNA Polymerase 300 units 4,163 EP0161 Terminal Deoxynucleotidyl Transferase 500 units 4,084 B62 Product Description Size Price(Rs.) Terminal Deoxynucleotidyl Transferase 2500 units 16,337 10X Reaction Buffer for phi29 DNA Polymerase 5x1 ml 1,021 Nucleases Deoxyribonuleases ( DNases) EN0521 Deoxyribonuclease I (DNase I), RNase-free 1000 units 3,299 EN0523 Deoxyribonuclease I (DNase I), RNase-free, HC 1000 units 3,299 EN0525 Deoxyribonuclease I (DNase I), RNase-free, supplied with MnCl2 1000 units 3,613 EN0591 Endonuclease IV, E.coli 100 units 11,781 EN0141 Endonuclease V, T.maritima 250 units 5,105 EN0581 Exonuclease I, E.coli 4000 units 4,791 EN0582 Exonuclease I, E.coli 20000 units 19,164 EN0191 Exonuclease III, E.coli 4000 units 2,828 2008-09 Chemicals, Reagents & Kits CAT# 128 Call +91-11-45027000 Fax. +91-11-25419631 E-mail : [email protected] Lambda Exonuclease 1000 units Size Price(Rs.) 4,555 EN0562 Lambda Exonuclease 5000 units 18,222 Ribonucleases ( RNases) EN0201 Ribonuclease H, E.coli 100 units 9,111 EN0202 Ribonuclease H, E.coli 500 units 36,444 EN0531 Ribonuclease A (RNase A), DNase-free 10 mg 2,513 CAT# Product Description Size Price(Rs.) 10 units 2,435 Shrimp Alkaline Phosphatase 500 units 9,229 EF0221 Pyrophosphatase, Inorganic (from yeast) EF0511 EK0031 T4 Polynucleotide Kinase 500 units 2,356 EK0032 T4 Polynucleotide Kinase 2500 units 9,425 4,568 Other Enzymes EO0461 Agarase 100 units EN0541 Ribonuclease T1 100000 units 3,927 ET0211 DNA Topoisomerase I 200 units Discontinue EN0542 Ribonuclease T1 500000 units 12,802 EO0491 Proteinase K 1 ml 2,749 EN0551 RNase A/T1 Mix 1 ml 4,870 EO0492 Proteinase K 5x1 ml 11,389 EN0601 Ribonuclease I, E.coli 1000 units 5,498 EN0361 Uracil - DNA Glycosylase 200 units 3,142 EN0602 Ribonuclease I, E.coli 5000 units Sugar Nonspecific Nuclease 21,992 EN0362 Uracil - DNA Glycosylase 5x200 units 12,567 EN0181 Micrococcal Nuclease EN0321 S1 Nuclease 8000 units 4,006 PCR, qPCR, RT PCR & dNTPs 10000 units 2,121 Standard PCR Ligases EP0701 DreamTaq™ DNA Polymerase 200 units NEW 2,356 EP0702 DreamTaq™ DNA Polymerase 500 units NEW 4,713 EP0703 DreamTaq™ DNA Polymerase 5x500 units NEW18,850 EP0704 DreamTaq™ DNA Polymerase20x500 units NEW 62,834 EL0014 T4 DNA Ligase 200 units 2,513 EL0011 T4 DNA Ligase 1000 units 3,927 EL0012 T4 DNA Ligase 5x1000 units 15,708 EL0334 T4 DNA Ligase (with PEG) 200 units 2,513 EL0331 T4 DNA Ligase (with PEG) 1000 units 3,927 Robust amplification with minimal optimization EL0017 T4 DNA Ligase, HC 1000 units 3,927 l EL0013 T4 DNA Ligase, HC 5000 units 15,708 EL0337 T4 DNA Ligase, HC (with PEG) 1000 units 3,927 EL0015 T4 DNA Ligase, LC 200 units 2,513 EL0016 T4 DNA Ligase, LC 5x200 units 3,927 EL0335 T4 DNA Ligase, LC (with PEG) 200 units 2,513 EL0336 T4 DNA Ligase, LC (with PEG) 5x200 units 3,927 EL0021 T4 RNA Ligase 3,927 B69 T4 DNA Ligase Reaction Buffer (supplied with T4 DNA Ligase) 1.5 ml DreamTaq™ DNA Polymerase l l l l 1000 units l 1,021 Phosphatases & Kinases EF0341 EF0342 EF0343 EF0654 EF0651 EF0652 NEW Robust amplification with minimal optimization. High yields of PCR products. Higher sensitivity compared to conventional Taq DNA polymerase. Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA. Compatible with TA cloning – Generates PCR products with 3’-dA overhangs. Incorporates modified nucleotides. EP0071 Taq DNA Polymerase (native, with BSA) 200 units 2,435 EP0072 Taq DNA Polymerase (native, with BSA) 500 units 9,739 200 units Discontinue EP0281 Calf Intestine Alkaline Phosphatase Taq DNA Polymerase (native, without BSA) 200 units 4,870 5x200 units Discontinue EP0282 Calf Intestine Alkaline Phosphatase,HC Taq DNA Polymerase (native, without BSA) 500 units 9,739 1000 units Discontinue EP0401 Taq DNA Polymerase (recombinant) 100 units 1,178 EP0402 Taq DNA Polymerase (recombinant) 500 units 4,713 EP0283 Taq DNA Polymerase, LC (native, without BSA) 200 units 4,870 EP0284 Taq DNA Polymerase, LC (native, without BSA) 500 units 9,739 EP0403 Taq DNA Polymerase, LC (recombinant) 100 units 1,178 EP0404 Taq DNA Polymerase, LC (recombinant) 500 units 4,713 EP0405 NEW Taq DNA Polymerase, recombinant 5x500u EP0406 NEW Taq DNA Polymerase, recombinant10x500u 18,850 FastAP™ Thermosensitive Alkaline Phosphatase 3000 rxn NEW 2,356 FastAP™ Thermosensitive EW Alkaline Phosphatase 1x1000 rxn N 4,320 FastAP™ Thermosensitive NEW Alkaline Phosphatase 5x1000 rxn 17,270 FastAP™ Thermosensitive Alkaline Phosphatase • Fast dephosphorylation: 10 min at 37°C • Fast thermoinactivation: 5 min at 75°C • Active in buffers for restriction enzymes and PCR • Does not require additives or buffer change • Single protocol for all types of DNA ends (blunt, 5’- and 3’- overhangs) Complete thermoinactivation of • Recombinant enzyme FastAP™ Thermosensitive Alkaline Phosphatase in 5 min at 75°C E-mail : [email protected] 33,930 K0171 2X PCR Master Mix 200 react 5,184 K0172 2X PCR Master Mix NEW 1000 react 20,735 Fax. +91-11-25419631 Call +91-11-45027000 129 2008-09 Calf Intestine Alkaline Phosphatase Chemicals, Reagents & Kits Product Description EN0561 Modifying Enzymes/Standard PCR CAT# Size Price(Rs.) CAT# Product Description Size Price(Rs.) PCR, qPCR & RT-PCR Product Description 2008-09 Chemicals, Reagents & Kits CAT# 130 Call +91-11-45027000 Fax. +91-11-25419631 E-mail : [email protected] Product Description Size Price(Rs.) Product Description Size Price(Rs.) PyroStart™ Fast PCR Master Mix (2X) Maxima™ SYBR Green NEW qPCR master Mix (2x) 200 react Only 18 min with any thermal cycler 10,800 K0222 Maxima™SYBR Green NEW qPCR master Mix (2x) 1000 react 50,071 K0231 Maxima™Probe qPCR NEW 200 react master Mix (2x) 10,800 K0232 NEW Maxima™Probe qPCR master Mix (2x)1000 react 50,071 Hot Start PCR EP0601 Maxima™ Hot Start Taq NEW DNA Polymerase 100 units 2,356 EP0602 Maxima™ Hot Start Taq EW N DNA Polymerase 500 units 9,425 EP0603 Maxima™ Hot Start Taq DNA Polymerase EP0611 TrueStart™ Taq DNA Polymerase 200 units 2,356 EP0612 TrueStart™ Taq DNA Polymerase 500 units 9,425 EP0613 TrueStart™ Taq DNA Polymerase 5x500 units 37,700 2x1.25 ml 10,210 5x500 units NEW 37,700 • Fast – reduces PCR reaction times to as low as 18 minutes with any thermal cycler. • Hot start PCR – high specificity and sensitivity of amplification. • Convenient room temperature PCR set-up. • Master mix format – lower risk of contamination and pipetting errors. • Efficient – amplifies genomic DNA targets up to 4 kb. • Compatible with TA cloning – Generates PCR products with 3’-dA overhangs. Applications • High throughput PCR. • Highly specific amplification of complex genomic and cDNA templates. • Amplification of low copy number templates. • RT-PCR. • Colony PCR. • TA cloning. PCR, qPCR & RT-PCR K0221 CAT# Fast PCR K0211 Pyrostart™ Fast PCR Master Mix (2X) High Fidelity PCR K0191 High Fidelity PCR Enzyme Mix100 units 4,477 K0192 High Fidelity PCR Enzyme Mix500 units 17,908 EP0571 Pfu DNA Polymerase (native) 100 units 4,320 EP0572 Pfu DNA Polymerase (native) 500 units 17,279 EP0501 Pfu DNA Polymerase (recombinant) 100 units 3,534 EP0502 Pfu DNA Polymerase (recombinant) 500 units 14,138 Long Range PCR K0181 Long PCR Enzyme Mix 100 units 4,477 K0182 Long PCR Enzyme Mix 500 units 17,908 K0201 Long PCR Control Set 25 react 2,356 Long PCR Enzyme Mix Ideal for GC-rich templates up to 85% B33 10X Taq Buffer with (NH4)2SO4 5x1 ml 1,021 B34 10X Taq Buffer with (NH4)2SO4 and 20mM MgCl2 5x1 ml 1,021 B38 10X Taq Buffer with KCl 5x1 ml 1,021 B16 10X Taq Buffer with KCl and 15mM MgCl2 5x1 ml 1,021 B55 10X Taq Buffer without Detergent 5x1 ml 1,021 B15 10X Taq Buffers with KCl Set B65 10X DreamTaq Buffers R0971 25mM MgCl 2 B14 Bovine Serum Albumin R0581 Water, nuclease-free R0582 Water, nuclease-free 30 ml 1,885 l l l E-mail : [email protected] Fax. +91-11-25419631 6x1 ml 1,021 4x1.25 ml 1,335 5x1 ml 1,021 5 mg 1,100 5x1 ml 1,021 Call +91-11-45027000 131 2008-09 Unique blend of two thermostable DNA polymerases. l Long PCR products: – up to 47 kb with viral DNA as template; – up to 21 kb with genomic DNA as template. l Ideal for GC-rich templates up to 85%. Fidelity is three-times higher than with Taq DNA polymerase. High yields. PCR Buffers and Reagents Chemicals, Reagents & Kits CAT# Real-time PCR Nucleotides/First Strand cDNA Synthesis Product Description Size Price(Rs.) Nucleotides CAT# Product Description Size Price(Rs.) Modified Nucleotides Deoxiribonuleoside Triphosphate R0072 ddNTP Set, 5mM Solutions 4x0.2 ml 6,440 R0141 dATP, 100mM Solution 0.25 ml 2,435 R0073 ddNTP Set, 20mM Solutions4x0.05 ml 6,440 R0151 dCTP, 100mM Solution 0.25 ml 2,435 R0421 dm 4CTP, 10mM Solution 0.1 ml 2,042 R0161 dGTP, 100mM Solution 0.25 ml 2,435 R0431 dm CTP, 10mM Solution 0.1 ml 2,042 R0171 dTTP, 100mM Solution 0.25 ml 2,435 R0501 dm 6ATP, 10mM Solution 0.1 ml 2,042 R0133 dUTP, 100mM Solution 0.25 ml 2,121 R1191 dITP, 100mM Solution 0.25 ml 3,534 R0241 dNTP Mix, 2mM each 1 ml 1,335 R0242 dNTP Mix, 2mM each 5x1 ml 5,341 R0191 dNTP Mix, 10mM each 0.2 ml 1,335 R0192 dNTP Mix, 10mM each 1 ml 5,341 R0193 dNTP Mix, 10mM each 5x1 ml 21,363 R1121 dNTP Mix, 25mM each 1 ml 12,567 R1122 dNTP Mix, 25mM each 5x1 ml 50,267 R0251 dNTP/dUTP Mix 1 ml 1,335 R0181 dNTP Set, 100mM Solutions 4x0.25 ml 7,304 R0182 dNTP Set, 100mM Solutions 4x1 ml 25,919 R0186 dNTP Set, 100mM Solutions 4x5 ml 1,02,105 5 First Strand cDNA Synthesis Reverse Trancriptases EP0641 AMV Reverse Transcriptase 1000 units 23,563 EP0351 M-MuLV Reverse Transcriptase 1000 units 4,713 EP0352 M-MuLV Reverse Transcriptase 5000 units 18,850 EP0451 RevertAid™ H Minus M-MuLV Reverse Transcriptase 10000 units 8,640 EP0452 RevertAid™ H Minus M-MuLV Reverse Transcriptase 5x10000 units 34,559 EP0441 RevertAid™ M-MuLV Reverse Transcriptase 10000 units 3,613 EP0442 RevertAid™ M-MuLV Reverse Transcriptase 5x10000 units 14,452 First strand cDNA synthesis Kits Ribonucleoside Triphosphate K1611 First Strand cDNA Synthesis Kit 20 react 4,477 17,279 R0441 ATP, 100mM Solution 0.25 ml 1,728 K1612 First Strand cDNA Synthesis Kit 100 react R0451 CTP, 100mM Solution 0.25 ml 1,728 K1621 R0471 UTP, 100mM Solution 0.25 ml 1,728 RevertAid™ First Strand cDNA Synthesis Kit 20 react 4,006 K1622 RevertAid™ First Strand cDNA Synthesis Kit 100 react 15,630 K1631 RevertAid™ H Minus First Strand cDNA Synthesis Kit 20 react 5,969 R0461 GTP, 100mM Solution 0.25 ml 1,728 R0481 NTP Set, 100mM Solutions 4x0.25 ml 5,027 2008-09 Chemicals, Reagents & Kits CAT# 132 Call +91-11-45027000 Fax. +91-11-25419631 E-mail : [email protected] RevertAid™ H Minus First Strand cDNA Synthesis Kit Size 100 react Price(Rs.) 22,306 Primers SO181 Exo-Resistant Random Primer SO131 SO132 SO142 100 µl 3,613 Oligo(dT)18 Primer 60 µl 3,613 Oligo(dT)18 Primer 120 µl 6,440 Random Hexamer Primer 120 µl 4,006 Primers For Sequencing SO101 SO115 SO100 SO113 SO114 SO501 SO511 SO116 SO117 2,121 M13/pUC Reverse Sequencing Primer (-46), 24mer 0.1 AU 2,121 M13/pUC Sequencing Primer (-20), 17-mer 0.1 AU 2,121 M13/pUC Sequencing Primer (-40), 17-mer 0.1 AU 2,121 M13/pUC Sequencing Primer (-46), 22-mer 0.1 AU 2,121 pJET1.2 Forward Sequencing Primer, 23-mer 0.2 AU 3,534 pJET1.2 Reverse Sequencing Primer, 24-mer 0.2 AU 3,534 SP6 Promoter Sequencing Primer, 18-mer 0.1 AU 2,121 0.1 AU 2,121 T3 Promoter Sequencing Primer, 17-mer 0.1 AU 2,121 SO120 T3 Promoter Sequencing Primer, 24-mer 0.1 AU 2,121 SO118 T7 Promoter Sequencing Primer, 20-mer 0.1 AU 2,121 MOLECULAR CLONING Kits for Ligation & Transformation K1421 K1422 Rapid DNA Ligation Kit Rapid DNA Ligation Kit Product Description Size Price(Rs.) K1423 Rapid DNA Ligation Kit 150 react 23,563 K1431 Rapid DNA Ligation & Transformation Kit 10 react 2,670 K1432 Rapid DNA Ligation & Transformation Kit 30 react 6,598 K2710 TransformAid™ Bacterial Transformation Kit 20 transformat 2,435 K2711 TransformAid™ Bacterial Transformation Kit 40 transformat 3,691 PCR Cloning Kits M13/pUC Reverse Sequencing Primer (-26), 17-mer 0.1 AU SP6 Promoter Sequencing Primer, 24-mer SO119 CAT# 10 react 50 react 2,356 9,425 CloneJET TM PCR Cloning Kit The CloneJET ™ PCR Cloning Kit K1231 CloneJET™ PCR Cloning Kit 20 react 8,483 K1232 CloneJET™ PCR Cloning Kit 40 react 16,023 K1213 InsTAclone™ PCR Cloning Kit 10 react 9,661 K1214 InsTAclone™ PCR Cloning Kit 30 react 14,530 Cloning Vectors & Phage DNA SD0011 Lambda DNA 500 µg 2,906 SD0021 Lambda DNA (dam¯, dcm¯ ) 500 µg 2,906 SD0041 pBR322 DNA 100 µg 4,398 SD0141 pTZ19R DNA 50 µg 4,398 SD0051 pUC18 DNA 50 µg 2,906 SD0061 pUC19 DNA 50 µg 2,906 SD0411 pUC19 DNA/BamHI 20 µg 4,241 SD0401 pUC19 DNA/SmaI 20 µg 4,241 SD0171 pUC57 DNA 50 µg 2,906 SD0031 φX174 RF1 DNA 50 µg 4,398 Microbial Media & Additives R0391 IPTG, dioxane-free 1g R0392 IPTG, dioxane-free 5g 5,891 R0393 IPTG, dioxane-free 5x5 g 20,421 R1171 IPTG Solution, ready-to-use 10x1.5 ml 3,927 R0401 X-Gal 3,849 R0404 X-Gal 1g 6,519 R0402 X-Gal 2x1 g 11,781 R0941 X-Gal Solution, ready-to-use 10 ml 7,069 M0011 FastMedia™ LB Liquid Amp20 pouches 15,708 M0021 FastMedia™ LB Agar Amp 20 pouches 18,261 M0031 FastMedia™ LB Agar 0.5 g Amp IPTG/X-Gal 20 pouches 1,964 22,581 Nucleic Acid Purification Kits for Purification K0502 E-mail : [email protected] 50 preps 5,694 K0503 GeneJET™ Plasmid Miniprep Kit 250 preps 22,581 K0512 Genomic DNA Purification Kit 100 preps 6,598 K0513 DNA Gel Extraction Kit 100 preps 5,027 Fax. +91-11-25419631 Call +91-11-45027000 133 2008-09 • Fast: clone PCR products in only 5 minutes • Highest Efficiency: >99% positive clones • No background: positive selection vector • Universal: any DNA fragment with any end structure can be cloned • New: T7 promoter for in vitro and in vivo expression GeneJET™ Plasmid Miniprep Kit Chemicals, Reagents & Kits Product Description K1632 Primers/Molecular cloning/Nucleic Acid Purification CAT# Nucleic Acid Purification/Molecular Labeling & Detection Product Description Size Price(Rs.) CAT# Product Description Size Price(Rs.) CAT# Product Description Size Price(Rs.) CAT# Product Description Size Price(Rs.) 2x0.1 ml 4,713 2x0.25 ml 3,534 Reagents Buffers and Solutions for GeneJet Plasmid Miniprep Kit R1263 Elution Buffer R0551 Glycogen, RNA grade R0561 Glycogen (from GeneJET™ Plasmid Miniprep Kit) R1223 R1233 R1213 R1253 R1243 134 30 ml 2,356 70 ml 2,749 Neutralization Solution (from GeneJET™ Plasmid Miniprep Kit) 100 ml 70 ml 2,749 RNase A (from GeneJET™ Plasmid Miniprep Kit) 0.7 ml 1,964 Call +91-11-45027000 K0651 Biotin DecaLabel™ DNA Labeling Kit 10 react 6,676 K0652 Biotin DecaLabel™ DNA Labeling Kit 30 react 16,494 K0621 DecaLabel™ DNA Labeling Kit 10 react 5,734 K0622 DecaLabel™ DNA Labeling Kit 30 react 8,640 2,749 Resuspension Solution (from GeneJET™ Plasmid Miniprep Kit) Wash Solution (from GeneJET™ Plasmid Miniprep Kit) Molecular Labeling and Detection Kits for Labeling Lysis Solution (from GeneJET™ Plasmid Miniprep Kit) 2008-09 Chemicals, Reagents & Kits CAT# Nucleotides for Labeling 100 ml 3,142 R0091 Aminoallyl-dUTP, 10mM Solution0.1 ml 6,283 R1101 Aminoallyl-dUTP, 50mM Solution50 µl 9,032 Fax. +91-11-25419631 E-mail : [email protected] Aminoallyl-UTP, 50mM Solution 50 µl Size R0081 Biotin-11-dUTP, 1mM Solution R0101 Fluorescein-12-dUTP, 1mM Solution25 µl Price(Rs.) 50 µl 9,032 Biotin Chromogenic Detection Kit10 react DNA Marker I for Genomic DNA Analysis 6 µg 3,770 SM0351 DNA Marker II for Genomic DNA Analysis 6 µg 3,770 SM1123 FastRuler™ DNA Ladder, High Range, ready-to-use 2x500 µl 4,006 SM1103 FastRuler™ DNA Ladder, Low Range, ready-to-use 2x500 µl 4,006 SM1113 FastRuler™ DNA Ladder, Middle Range, ready-to-use 2x500 µl 4,006 SM1233 FastRuler™ DNA Ladder, Ultra Low Range, ready-to-use 2x500 µl 4,713 SM0241 GeneRuler™ 100bp DNA Ladder 50 µg 3,220 SM0242 GeneRuler™ 100bp DNA Ladder 5x50 µg 12,881 SM0244 GeneRuler™ 100bp DNA Ladder, ready to use 250µg 19,793 SM0321 GeneRuler™ 100bp DNA Ladder Plus 50 µg 3,299 SM0322 GeneRuler™ 100bp DNA Ladder Plus 5x50 µg 13,195 SM0323 GeneRuler™ 100bp DNA Ladder Plus, ready-to-use SM0324 GeneRuler™ 100bp Plus DNA Ladder, ready-to-use 7,069 Biotin Chromogenic Detection Kit30 react 1g 8,051 R0822 BCIP-T 5g 32,595 R0841 NBT 1g 7,461 R0842 NBT 5g 30,239 M1001 SensiBlot™ Plus Nylon 10 sheets 34,951 16,494 SensiBlot™ Plus Nylon 1 roll DNA Electrophoresis SM0341 BCIP-T Membrane (30cmx3m) Price(Rs.) DNA Ladders and Markers R0821 M1002 Size 10,210 K0662 Membrane (20x20cm) Product Description 10,210 Blotting and Detection K0661 CAT# 41,235 IN VITRO TRANSCRIPTION Kits for Transcription K0411 T7 Transcription Kit 10 react 2,121 K0412 T7 Transcription Kit 30 react 5,969 K0441 TranscriptAid™ T7 High Yield Transcription Kit 50 react 20,421 50 µg 3,927 250 µg 19,793 50 µg 3,849 SM0243 GeneRuler™ 100bp DNA Ladder, ready-to-use SM0311 GeneRuler™ 1kb DNA Ladder 5x50 µg 5,576 TranscriptAid™ T7 High Yield Transcription Kit, #K0441, 50 reactions SM0312 GeneRuler™ 1kb DNA Ladder 25x50 µg 22,306 SM1331 GeneRuler™ 1kb DNA Ladder Plus 5x50 µg 6,912 Highest yield and integrity of RNA transcripts SM1332 GeneRuler™ 1kb DNA Ladder Plus 25x50 µg 27,647 SM1333 GeneRuler™ 1kb DNA Ladder Plus, ready-to-use SM1334 GeneRuler™ 1kb Plus DNA Ladder , ready-to-use Optimal solution for all your transcription demands! Enzymes for Transcription RNA Polymerases EP0131 SP6 RNA Polymerase 2000 units 4,320 EP0133 SP6 RNA Polymerase, HC 5000 units 10,839 EP0101 T3 RNA Polymerase 2000 units 2,356 EP0102 T3 RNA Polymerase 10000 units 9,425 EP0103 T3 RNA Polymerase, HC 10000 units 9,425 EP0111 T7 RNA Polymerase 5000 units 4,084 EP0112 T7 RNA Polymerase 5x5000 units 16,337 EP0113 T7 RNA Polymerase, HC 25000 units 16,337 Ribonuclease Inhibitors RiboLock™ RNase Inhibitor 2500 units EO0382 RiboLock™ RNase Inhibitor 4x2500 units EO0384 RiboLock™ RNase Inhibitor 24x2500 units E-mail : [email protected] 3,613 50 µg 3,534 SM0313 GeneRuler™ 1kb DNA Ladder, ready-to-use 5x50 µg 6,362 SM0314 GeneRuler™ 1kb DNA Ladder, ready-to-use 50 µg 3,534 SM0371 GeneRuler™ 50bp DNA Ladder 50 µg 3,849 SM0372 GeneRuler™ 50bp DNA Ladder 5x50 µg 15,394 SM0373 GeneRuler™ 50bp DNA Ladder, ready-to-use SM0331 GeneRuler™ DNA Ladder Mix 5x50 µg 5,498 SM0332 GeneRuler™ DNA Ladder Mix25x50 µg 21,992 SM0333 GeneRuler™ DNA Ladder Mix, ready-to-use 5x50 µg 6,283 SM0334 GeneRuler™ DNA Ladder Mix, ready-to-use 50 µg 3,534 SM1351 GeneRuler™ DNA Ladder, High Range 50 µg 5,891 SM1352 GeneRuler™ DNA Ladder, High Range 5x50 µg 23,563 SM1353 GeneRuler™ DNA Ladder, High Range, ready-to-use 50 µg 7,226 SM1191 GeneRuler™ DNA Ladder, Low Range 50 µg 4,163 SM1192 GeneRuler™ DNA Ladder, Low Range 5x50 µg 16,651 SM1193 GeneRuler™ DNA Ladder, Low Range, ready-to-use 50 µg 5,184 SM1211 GeneRuler™ DNA Ladder, Ultra Low Range 50 µg 4,163 SM1212 GeneRuler™ DNA Ladder, Ultra Low Range 12,802 57,964 8,640 Fax. +91-11-25419631 50 µg 5x50 µg Call +91-11-45027000 4,870 16,651 135 2008-09 EO0381 5x50 µg Chemicals, Reagents & Kits Product Description R1091 In Vitro Transcription/DNA Ladders & Markers CAT# Size Price(Rs.) CAT# Product Description Size Price(Rs.) CAT# Product Description Size Price(Rs.) CAT# Product Description SM1213 GeneRuler™ DNA Ladder, Ultra Low Range, ready-to-use 50 µg 5,184 SM1551 GeneRuler™ Express DNA Ladder 50 µg SM1552 GeneRuler™ Express DNA Ladder250 µg DNA Ladders & Markers Product Description SM1553 SM0161 GeneRuler™ Express DNA Ladder, ready-to-use Size Price(Rs.) SM0103 Lambda DNA/HindIII Marker, ready-to-use 5x50 µg 2,906 3,927 SM0361 Lambda DNA/PstI Marker 5x50 µg 2,749 15,708 SM0231 Lambda Mix Marker 5x50 µg 2,749 SM0291 Lambda-pUC Mix Marker 50 µg 2,749 SM0393 MassRuler™ DNA Ladder, High Range, ready-to-use 2x500 µl 6,126 SM0383 MassRuler™ DNA Ladder, Low Range, ready-to-use 2x500 µl 6,126 SM0403 MassRuler™ DNA Ladder, Mix, ready-to-use 2x500 µl 6,756 SM1283 MassRuler™ Express DNA Ladder Mix, Forward, ready-to-use 2x500 µl 7,069 SM1293 MassRuler™ Express DNA Ladder Mix, Reverse, ready-to-use 2x500 µl 7,069 SM1243 MassRuler™ Express DNA Ladder, HR Forward, ready-to-use 2x500 µl 6,440 50 µg 5,105 Lambda DNA/Eco130I Marker 5x50 µg 2,749 SM1051 Lambda DNA/Eco47I (AvaII) Marker 5x50 µg 2,749 SM0111 Lambda DNA/Eco91I Marker 5x50 µg 2,749 SM0281 Lambda DNA/EcoRI Marker 5x50 µg 2,749 SM0191 2008-09 Chemicals, Reagents & Kits CAT# Lambda DNA/ EcoRI+HindIII Marker 5x50 µg SM0192 Lambda DNA/ EcoRI+HindIII Marker SM0193 Lambda DNA/ EcoRI+HindIII Marker, ready-to-use5x50 µg 25x50 µg 2,749 10,996 2,906 SM0101 Lambda DNA/HindIII Marker 5x50 µg 2,749 SM0102 Lambda DNA/HindIII Marker 25x50 µg 10,996 136 Call +91-11-45027000 Fax. +91-11-25419631 E-mail : [email protected] MassRuler™ Express DNA Ladder, HR Reverse, ready-to-use SM1263 MassRuler™ Express DNA Ladder, LR Forward, ready-to-use SM1273 MassRuler™ Express DNA Ladder, LR Reverse, ready-to-use SM1751 SM1671 Size 2x500 µl 2x500 µl Price(Rs.) 6,440 6,440 2x500 µl 6,440 NoLimits™ 10000bp DNA Fragment 10 µg 3,927 NoLimits™ 1000bp DNA Fragment 10 µg 3,927 SM1441 NoLimits™ 100bp DNA Fragment 10 µg 3,927 SM1391 NoLimits™ 10bp DNA Fragment 10 µg 3,927 SM1681 NoLimits™ 1200bp DNA Fragment 10 µg 3,927 CAT# Size Price(Rs.) SM1621 Product Description NoLimits™ 300bp DNA Fragment 10 µg 3,927 SM1501 NoLimits™ 3500bp DNA Fragment 10 µg 3,927 SM1411 NoLimits™ 35bp DNA Fragment 10 µg 3,927 SM1721 NoLimits™ 4000bp DNA Fragment 10 µg 3,927 SM1631 NoLimits™ 400bp DNA Fragment 10 µg 3,927 SM1731 NoLimits™ 5000bp DNA Fragment 10 µg 3,927 SM1641 NoLimits™ 500bp DNA Fragment 10 µg 3,927 SM1421 NoLimits™ 50bp DNA Fragment 10 µg 3,927 SM1511 NoLimits™ 6000bp DNA Fragment 10 µg 3,927 SM1461 NoLimits™ 600bp DNA Fragment 10 µg 3,927 SM1741 NoLimits™ 7000bp DNA Fragment 10 µg 3,927 SM1651 NoLimits™ 700bp DNA Fragment 10 µg 3,927 SM1471 NoLimits™ 750bp DNA Fragment 10 µg 3,927 NoLimits™ 15000bp DNA Fragment 10 µg 3,927 SM1691 NoLimits™ 1500bp DNA Fragment 10 µg 3,927 SM1431 NoLimits™ 75bp DNA Fragment 10 µg 3,927 SM1601 NoLimits™ 150bp DNA Fragment 10 µg 3,927 SM1521 NoLimits™ 8000bp DNA Fragment 10 µg 3,927 SM1381 NoLimits™ 15bp DNA Fragment 10 µg 3,927 SM1481 NoLimits™ 800bp DNA Fragment 10 µg 3,927 SM1771 NoLimits™ 17000bp DNA Fragment 10 µg 3,927 SM1661 NoLimits™ 850bp DNA Fragment 10 µg 3,927 SM1541 NoLimits™ 20000bp DNA Fragment 10 µg 3,927 SM1491 NoLimits™ 900bp DNA Fragment10 µg 3,927 SM1701 NoLimits™ 2000bp DNA Fragment 10 µg 3,927 SM1153 SM1611 NoLimits™ 200bp DNA Fragment 3,927 O’GeneRuler™ 100bp DNA Ladder Plus, ready-to-use 50 µg 3,927 SM1401 NoLimits™ 20bp DNA Fragment 10 µg 3,927 SM1571 NoLimits™ 2500bp DNA Fragment 10 µg 3,927 O’GeneRuler™ 100bp DNA Ladder, ready-to-use 50 µg 3,927 SM1451 NoLimits™ 250bp DNA Fragment 10 µg 3,927 O’GeneRuler™ 1kb DNA Ladder Plus, ready-to-use 5x50 µg 8,640 SM1761 NoLimits™ 25bp DNA Fragment 10 µg 3,927 SM1711 NoLimits™ 3000bp DNA Fragment 10 µg 3,927 O’GeneRuler™ 1kb DNA Ladder, ready-to-use 5x50 µg 6,440 E-mail : [email protected] 10 µg SM1143 SM1343 SM1163 Fax. +91-11-25419631 Call +91-11-45027000 137 2008-09 SM1531 Chemicals, Reagents & Kits Product Description SM1253 DNA Ladders & Markers CAT# DNA Ladders & Markers/Reagents for DNA Electrophoresis Product Description Size Price(Rs.) CAT# Product Description CAT# Product Description Size Price(Rs.) CAT# Product Description SM1133 O’GeneRuler™ 50bp DNA Ladder, ready-to-use SM1173 SM1203 SM1223 SM1563 SM1313 SM1323 SM1303 O’GeneRuler™ DNA Ladder Mix, ready-to-use O’GeneRuler™ DNA Ladder, Low Range, ready-to-use O’GeneRuler™ DNA Ladder, Ultra Low Range, ready-to-use O’GeneRuler™ Express DNA Ladder, ready-to-use O’Range Ruler™ 10bp DNA Ladder, ready-to-use O’Range Ruler™ 20bp DNA Ladder, ready-to-use O’Range Ruler™ 5bp DNA Ladder, ready-to-use 50 µg 5,027 5x50 µg 6,440 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg 25 µg 5,576 O’RangeRuler™ 500bp DNA Ladder, ready-to-use 25 µg 25 µg 50 µg 5,262 SM0252 PhiX174 DNA/BsuRI (HaeIII) Marker 5x50 µg 21,049 SM0253 PhiX174 DNA/BsuRI (HaeIII) Marker, ready-to-use 50 µg 6,440 SM0261 PhiX174 DNA/HinfI Marker 50 µg 5,341 Reagents for DNA Electrophoresis 10X Loading Dye Solution (for Genomic DNA Analysis) 1 ml 1,021 5,576 R0611 6X Loading Dye Solution 5x1 ml 1,964 R0621 6X MassRuler™ Loading Dye Solution 5x1 ml 1,964 R0631 6X Orange Loading Dye Solution 5x1 ml 1,964 B49 50X TAE Electrophoresis Buffer 1 l 5,184 B52 10X TBE Electrophoresis Buffer 1 l 2,828 R0491 TopVision™ LE GQ Agarose (low electroendosmosis) 100 g 16,887 R0801 TopVision™ LM GQ Agarose (low melting point) 25 g 14,530 2,749 Call +91-11-45027000 8,640 PhiX174 DNA/BsuRI (HaeIII) Marker R1051 50 µg 138 2x50 µg SM0251 5,576 pBR322 DNA/AluI Marker, ready-to-use 50 µg 2,906 ZipRuler™ Express DNA Ladder Set, ready-to-use 2,121 pBR322 DNA/AluI Marker pUC Mix Marker 50 µg SM1373 2,121 SM0123 SM0301 10,996 pUC19 DNA/MspI (HpaII) Marker, ready-to-use 6X TriTrack™ Loading Dye Solution 5x1 ml SM0121 50 µg 5x50 µg SM0223 6X Loading Dye & SDS Solution 5x1 ml 5,969 pBR322 DNA/BsuRI (HaeIII) Marker 2,749 pUC19 DNA/MspI (HpaII) Marker R1161 25 µg SM0271 pUC19 DNA/MspI (HpaII) Marker 50 µg SM0222 R1151 O’RangeRuler™ 50bp DNA Ladder, ready-to-use 50 µg SM0221 7,854 5,576 SM0643 3,770 pUC Mix Marker, ready-to-use 6,283 25 µg O’RangeRuler™ 200bp DNA Ladder, ready-to-use 13,195 50 µg SM0303 7,069 O’RangeRuler™ 100bp+500bp DNA Ladder, ready-to-use SM0633 Price(Rs.) 5,105 SM0653 Price(Rs.) Size pUC Mix Marker 5,184 O’RangeRuler™ 100bp DNA Ladder, ready-to-use Size 5x50 µg SM0302 5,184 SM0623 SM0613 2008-09 Chemicals, Reagents & Kits CAT# 2,906 2,749 3,299 Fax. +91-11-25419631 E-mail : [email protected] Product Description Size Price(Rs.) CAT# Size Price(Rs.) Protein Ladders (10 – 260 kDa) RNA ladders SM1821 RiboRuler™ RNA Ladder, High Range 5x20 µl SM1823 RiboRuler™ RNA Ladder, High Range, ready-to-use 9,189 5x40 µl 10,525 SM1831 RiboRuler™ RNA Ladder, Low Range 5x20 µl 8,168 SM1833 RiboRuler™ RNA Ladder, Low Range, ready-to-use 9,504 5x40 µl Ragents for RNA Electrophoresis R0641 2X RNA Loading Dye Solution 1 ml 1,885 Protein Electrophoresis & Analysis Protein Extraction and Quantification K0301 ProteoJET™ Mammalian Cell Lysis Reagent K0311 ProteoJET™ Cytoplasmic & Nuclear Protein NEW Extraction Kit 50 samples 6,676 R1271 Bradford Reagent, ready to use 1000 ml NEW 6,283 R1281 Bovine Serum Albumin, Standard set, NEW ready to use 7 conc. 4x1 ml 7,069 R1291 R0882 1x250 ml NEW 9,032 Bovine Gamma Globulin, Standard set, ready to use 7 conc. 4x1 ml NEW 7,069 Protein Standard Solution 10x1 ml *Each protein of the PageRuler™ Unstained Protein Ladder (#SM0661) contains an integral Strep-tag® II sequence. This sequence can be detected directly on Wester blots using reporter enzyme conjugated with Strep-Tactin® or Strep-tag® II specific antibodies SM0431 Unstained Protein Molecular Weight Marker 2x1000 µl 2,906 R0571 PageBlue™ Protein Staining Solution 1 l 6,283 K0681 PageSilver™ Silver Staining Kit 25 appl 8,640 Protein Detection 2,828 Buffer For Protein Sample Loading R1011 DualColor™ Protein Loading Buffer Pack 1000 samples of R0891 Protein Loading Buffer Pack EW 40 µl N 3,142 1 pack 2,828 Buffer For Protein Electrophoresis B46 10X Tris-glycine-SDS Electrophoresis Buffer 1 l 2,828 B47 10X Tris-glycine Electrophoresis Buffer 1 l 2,828 B48 10X Tris-tricine-SDS Electrophoresis Buffer 1 l 6,048 RNA Electrophoresis/Protein Electrophoresis & Analysis RNA Electrophoresis Product Description DualColor™ Protein Loading Buffer Pack, #R1011 Prestained Protein Ladders and Marker SM0671 l PageRuler™ Prestained Protein Ladder 2x250 µl 7,461 SM0672 PageRuler™ Prestained Protein Ladder 2.5 ml 30,160 SM1811 PageRuler™ Prestained Protein Ladder Plus 2x250 µl 7,854 2.5 ml 31,417 2x250 µl 2,906 PageRuler™ Plus Prestained Protein Ladder SM0441 Prestained Protein Molecular Weight Marker SM1841 Spectra™ Multicolor NEW Broad Range Protein Ladder 2x250 µl 7,069 2008-09 SM1812 l Protects proteins from degradation during sample preparation for SDS-PAGE Convenient: two dyes (blue and pink) allow for: – tracking the progress of electrophoresis – monitoring of Western blot protein transfer Unstained Protein Ladders and markers SM0661 PageRuler™ Unstained Protein Ladder E-mail : [email protected] 2x250 µl 4,320 Fax. +91-11-25419631 Call +91-11-45027000 139 Chemicals, Reagents & Kits CAT# Transfection Reagents/Molecular Biology Grade Reagents 2008-09 Chemicals, Reagents & Kits CAT# Product Description Size Price(Rs.) CAT# TurboFect™ in vitro Transfection Reagent Product Description Size Price(Rs.) High transfection efficiency of a wide variety of cell types: Primary and secondary cell lines, l Adherent and suspension cells, l Differentiated and undifferentiated cells, l Stable and transient transfections. l No optimization required for most cell lines. l 1 ml delivers 500 µg of DNA. l Excellent transfection efficiency in the presence or absence of serum. l Minimal cytotoxicity. l Ready-to-use reagent - no need to reconstitute, dilute or manipulate. l Simple protocol. l l Comparison of Fermentas TurboFect™ in vitro transfection reagent with tranfection reagents from other vendors. HeLa cells were transfected with plasmid DNA encoding eGFP. Transfections were performed according to manufacturers recommendations and analysed by flow cytometry. CAT# Product Description Size Price(Rs.) TRANSFECTION REAGENTS R0511 ExGen 500 in vitro Transfection Reagent R0521 ExGen 500 in vivo Transfection Reagent R0531 TurboFect™ in vitro Transfection Reagent 1 ml 0.1 ml NEW 1 ml 25,133 51,052 13,352 MOLECULAR BIOLOGY GRADE REAGENTS CAT# Product Description Size Price(Rs.) R0811 5-Fluoroorotic Acid 1g 9,229 R0812 5-Fluoroorotic Acid 5g 36,915 R0851 X-Gluc 0.1 g 7,697 R0852 X-Gluc 0.5 g 30,789 R0861 DTT 5g 5,694 R0862 DTT 25 g 23,170 R1021 0.5M EDTA, pH 8.0 5x1 ml 1,021 E.coli STRAINS R0601 DEPC-treated Water 30 ml 2,042 R0603 DEPC-treated Water 5x1 ml 1,178 M0099 E.coli GM2163 POR M0109 E.coli JM107 POR Recommendations for Transfection I. DNA Quality Requirements High quality of DNA is critical for successful transfection. An A260/A280 ratio of 1.8, or greater is recommended. Endotoxincontaminated DNA may result in inefficient transfection and cause unacceptably high cellular toxicity. II. Cell Density The recommended confluency for adherent cells at the day of transfection is 50-70%. Suspension cells should be plated at an optimal density ensuring their logarithmic growth at the time of transfection. III. Incubation Time Transient transgene expression takes place within 2-72 hours after transfection. The optimal time depends on the cell type, promoter strength and expression product, and has to be determined experimentally. IV. Choice of Promoter High transfection efficiency depends both on the transgene promoter and on the cell line used. Cytomegalovirus (CMV) promoter is commonly used for high gene expression in a variety of cell lines. Other promoters, such as those from simian virus (SV40) and from Rous sarcoma virus (RSV) can also be used. V. Transfection Reagent/DNA Ratio The amount of transfection reagent used in transfection depends on the amount of DNA, transgene and cells to be transfected. The ratio presented in the protocol on page 470 are starting ratios and can be further optimized for best results. VI. Transfection in the Presence of Serum Transfection efficiency is often significantly lower in the presence of serum when using other transfection reagents. This is particularly true when cationic lipids are used as vectors for gene transfer. However, the transfection efficiency with Fermentas transfection reagents is consistently high in the presence of serum. VII. Centrifugation Gentle centrifugation of tissue culture plates for 5 minutes at 280 x g after addition of the polyplexes can improve transfection efficiency. 140 Call +91-11-45027000 Fax. +91-11-25419631 E-mail : [email protected] Product Description Size Price(Rs.) CAT# Product Description Size Chemicals, Reagents & Kits CAT# Price(Rs.) 2008-09 E-mail : [email protected] Fax. +91-11-25419631 Call +91-11-45027000 141
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