Environmental Studies FACT SHEET www.curriculum-press.co.uk Number 178 Do we still need vegetative propogation? This Factsheet: • Outlines the process of vegetative reproduction in potatoes • Describes the advantages of vegetative reproduction • Discusses the possible advantages and problems of GM potatoes • Reviews recent exam questions on this topic Vegetative reproduction is asexual reproduction i.e. it doesn’t involve fertilisation. Most plants can carry out some form of vegetative reproduction and a few plants only use this technique. Humans exploit vegetative reproduction in two main ways: • By taking cuttings to grow e.g. houseplants, potatoes, bananas and oranges • By taking cells or tissues from a plant and using them to grow genetically identical clones of the old plant Advantages of vegetative reproduction The plants that are produced are genetically identical to the parent and each other so useful characteristics e.g. size, shape, taste, disease resistance are all the same. The new, young plant gets its food from part of the parent plant so isn’t as reliant on nutrients in the soil. As a result, survival rates are high. Case Study: Potatoes The potato Solanum tuberosum originates from the Andes mountains in South America, is the most important ‘root crop’ grown in the world and is well adapted for survival in a cool climate like the UK. Despite this, it is estimated that 30% of the worldwide potato crop is lost each year to disease: Fungi such as as late blight (Phytophthora infestans), attack the foliage; fungal and bacterial diseases cause wilting and early death of plants; Many viruses attack systemically i.e. travel through the xylem and phloem of the plant Source: USDA For these reasons genetic engineers have been trying to develop genetically modified varieties for decades. There have been success stories but in Europe many environmentalists remain strongly opposed to any GM trials, including those using GM potatoes. Why are potatoes so important? After wheat and rice, they are the 3rd most widely grown species on Earth They are high in complex carbohydrates, fibre, protein, vitamins and minerals and contain virtually no fat The tuber-bearing genus Solanum is a huge genetic resource – there are 230 wild and primitive species within the genus so finding genes that control useful characteristics is relatively easy Vegetative reproduction of potatoes Although potatoes can reproduce sexually using seeds, humans grow them vegetatively i.e. asexually. Potatoes are propagated vegetatively using small “seed” potatoes that possess at least one axillary bud. This bud will grow into a new potato plant using the energy contained in the seed poato. Follow the numbers around Fig.1 (p2). 1 Environmental Studies Factsheet 178 - Do we still need vegetative propagation? Fig. 1 Vegetative propagation of potatoes Seed potato planted in soil 1 Aerial parts die in first autumn frost and gardener digs their potatoes up One potato has turned into 8! 2 8 Bud develops into shoot that emerges from soil Aerial parts photosynthesise i.e. turn CO2 from the air and water from the soil and potato into new tissue 3 4 7 5 Gardener piles soil against the newly emerged stem (“earthing up”) Tuber shrinks as the new plant digests its starch store for energy to grow 6 New tubers (potatoes) develop using energy from photosynthesis of aerial parts Normally, each “seed” potato will have several buds, each of which may develop into a new plant, so productivity is high. Genetically engineered potatoes So, if vegetative reproduction is so great, why would we want genetically engineered potatoes? Typical Exam Question (a) Potatoes are usually propagated vegetatively. What are the advantages of this approach? (3) (b) The diagram shows the energy ratio for a variety of crops. Because we can cut genes that control desired characteristics out of other plants and insert them into potatoes which then have the new, desired characteristic. Such characteristics include: • Improved protein content – important for populations suffering protein deficiency • Herbicide resistance – so that weeds that reduce yields can be killed without harming the potatoes • Increased resistance to disease – which reduce yield or kill the potatoes and might mean we could grow potatoes with less pesticide use • Increased tolerance of salt water – important in areas that are likely to be flooded by seawater • Increased drought tolerance – allowing potatoes to be grown where they otherwise couldn’t be grown • Genes that control the production of vaccines could be inserted into potatoes thus resulting in edible vaccines – much more attractive than needles! • A gene that produces a specific type of starch now can be used to make paper pulp, glue or animal feed – this GM potato has just been banned from sale in the EU These “improved” potatoes could then be reproduced by vegetative propagation. The energy ratio is given by: energy output energy input Suggest a reason for the energy ratio for potatoes (1) 100 60 tropical crops - subsistence 10.0 5.0 tropical crops 4.20 sugar beet UK 1.0 1.75 potatoes UK 0.90 peas UK 0.38 milk production UK 0.1 0.14 battery eggs UK The desired genes are cut out of the plants using enzymes and then a harmless virus is used to carry the desired gene into the potato. 0.04 fishing fleet UK Potatoes, as with all of our other staple crops have been improved over centuries by artificial selection – crossing plants with useful characteristics in the hope that they will be combined in the offspring – but GM is faster and more accurate. 0.01 2 Environmental Studies Factsheet 178 - Do we still need vegetative propagation? Possible dangers of GM potatoes Many environmentalists are concerned that genes inserted into potatoes may mutate resulting in unforeseen problems. There is also the possibility that these foreign genes might “jump” across other species, again with unforeseen consequences. For example, some are worried that a potato that has had a gene inserted that offers resistance to a particular disease may cross with a weed species – making the weed species disease resistant and reducing crop yields. Others are worried that introducing foreign genes – from other plant species or even animals or microorganisms – could lead to an increase in their allerginicity – their potential to cause allergic reactions. Scientists are carrying out trials across the world, including in the UK, to test whether these problems are real but campaigners have threatened to destroy the trial crops. Activity Using the info above and your own research, draw a spider diagram showing all the possible advantages and possible dangers of growing GM potatoes. Sources of info: Supporting GM – www.monsanto.com Against GM – www.greenpeace.org There are loads more! Mark scheme for Typical Exam Questions (a)Growing from seeds would produce more variation/ All potatoes are genetically identical / possess same useful characteristics as the parent; No sexual reproduction involved/no meiosis so only one parent needed; Ref to mitosis; Large numbers can be produced quickly; Asexual reproduction enables organisms to survive harsh conditions e.g. as spores or bulbs that can survive dormant in soil; (b)Energy obtained from “seed” potato/tuber as well as photosynthesis/ energy efficient variety chosen as “seed”; Practice Questions Potato plants originate from the Andes mountains in South America. They are adapted for survival in a cool climate. The potatoes we eat are food storage organs, called tubers, and are produced on underground stems. The graph shows the rates of photosynthesis and respiration of one common variety of potato plant. photosynthesis Rate respiration 0 5 10 15 20 25 30 Temperature/ oC Mark scheme (a) 7/8– 24°C; (b)Photosynthesis rate only just above/ equal to respiration rate; So little gain in biomass / net loss of biomass; Ref to energy being lost at night (via) respiration; Due to (night-time) respiration; No excess production for storage in tubers; (a) Between which temperatures is there a net gain of energy by the potato plant? (1) (b) When this variety was grown in a region with a mean daytime temperature of 24.0 °C, it failed to produce tubers. Suggest why (2) Acknowledgments: This Factsheet was researched and written by Kevin Byrne. Curriculum Press, Bank House, 105 King Street, Wellington, Shropshire, TF1 1NU Environmental Studies Factsheets may be copied free of charge by teaching staff or students, provided that their school is a registered subscriber. ISSN 1351-5136 3
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