CURRICULUM VITAE Yanguang Wu, PhD Personal Information Birthday: Feb 18, 1979 Marital Status: Married Major: Reproduction and Embryology Degree: PhD Working Address: The Center for human reproduction 21 E 69th street, New York, NY, 10021 Nationality: Permanent residency of USA (Green card) Telephone: 212‐994‐4400(Office); 312‐532‐7708(Cell) Email: [email protected] or [email protected] Education and Work experience 2002 ‐ 2007: PhD student, College of Life Science, Northeast Agricultural University, China. 2007 ‐ 2010: Postdoctoral research fellowship, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, China. 2010 ‐ 2011: Postdoctoral research associate, Department of Physiology and Biophysics, College of Medicine, University of Illinois at Chicago, U.S.A. 2011 ‐ 2013: Research Assistant Professor, Department of Physiology and Biophysics, College of Medicine, University of Illinois at Chicago, U.S.A. 2013 – now: Embryologist and Associate Scientist. Center for Human Reproduction, U.S.A Human IVF Experiences Since 05/01/2013, I started to perform clinical duty in The Center for Human Reproduction (CHR) as a full time Embryologist. As an experienced research scientist with 10 years experience in mammalian embryology and developmental biology, I finished my clinical training within 3 months, and then started working independently in CHR. By now, I have completed more than 700 IVF cycles independently in CHR. List of Human IVF Skills (Embryology and Andrology) 1 of 4 Manipulation of human gametes and embryos:  Oocyte retrieval, culture and assessment  Semen analysis and processing for IVF/ICSI  Conventional IVF and intracytoplasmic sperm injection (ICSI)  Fertilization assessment, embryo culture and embryo grade  Assisted zona hatching and embryo transfer  Day 3/5 embryo biopsy  Fluorescence in situ hybridization (FISH) for embryo gender identification and aneuploidy analysis Gamete and embryo cryopreservation:  Semen cryopreservation and thawing  Oocyte cryopreservation and thawing  Embryo cryopreservation and thawing, including slow freezing and vitrification Daily Laboratory maintenance and quality control  Medium and supplies management  Laboratory instruments daily check and maintenance  Quality control test and report  Medical record report and trouble shooting Research Experiences My research experiences and interests are concentrated on Mammalian Reproductive Endocrinology and Embryology including genomic epigenetic reprogramming in mammalian embryo development, mechanism of gametogenesis and steroidogenesis, regulation of gene expression in female ovarian cells, animal oocyte in vitro maturation, in vitro fertilization, sperm microinjection, embryo and tissue in vitro culture, cryopreservation and transfer. List of Research Skills Manipulation of mammalian gametes and embryos:  Superovulation and collection of oocyte or sperm  Oocyte in vitro maturation and fertilization or ICSI  Embryo culture, cryopreservation and transfer  Somatic cell nuclear transfer (SCNT) in mice and other animals)  Transgenic animal production by DNA‐pronuclear injection or SCNT Cell Biology and Molecular Biology:  Cell and tissue culture  Immunocytochemistry by using fluorescent or CLSM microscopy.  DNA, RNA and protein isolation.  PCR, western, EMSA and immunoprecipitation.  Plasmid construction and gene promoter activity analysis  RNA interference Professional Honors & Recognition 2007, Shandong Province Science and Technology Progress Award, China 2007, Outstanding Graduate Award, Northeast Agricultural University, China 2012, 2012 SSR outstanding presentation travel award, US 2 of 4 Professional Memberships Member, Society for Study of Reproduction Member, American Association of Bioanalysts Member, NY Metropolitan Embryology Society Grants Participated as a Major Researcher 1. Follicle atresia and cell apoptosis in the goat species, Grant from China National Natural Science Foundation (No. 30070556). 2. Germinal vesicle chromatin configuration in domestic animals, Grant from China National Natural Science Foundation (No. 30170679). 3. Molecular basis for meiosis, fertilization, and early development of domestic animals, Grant from China National Natural Science Foundation (No. 30430530). 4. Dialogue between somatic cell nuclei and the oocyte during nuclear transfer, Grant from the National Basic Research Project of the China Ministry of Science and Technology (No. 2006CB944003). 5. Chemically‐assisted enucleation of mammalian oocytes, Grant from China National Natural Science Foundation (No. 30771556). 6. Molecular control of follicle development and atresia, Grant from the National Basic Research Project of the China Ministry of Science and Technology (No. 2007CB947403). 7. Intracellular crosstalk between FSH, insulin and androgens on the regulation of aromatase expression in rat granulosa cells. CRB pilot Grant of university of Illinois at Chicago, USA Publication list (summary only): 1. Wu YG, Barad DH, Kushnir VA, Lazzaroni E, Wang Q, Albertini DF, Gleicher N. Aging-related premature luteinization of granulosa
cells is avoided by early oocyte retrieval. J Endocrinol 2015;226(3):167-80
2. Wu YG, Baumgarten S, Zhou P, and Stocco C. Testosterone-Dependent Interaction between Androgen Receptor and Aryl
Hydrocarbon Receptor Induces Liver Receptor Homolog 1 Expression in Rat Granulosa Cells. Mol Cell Biol 2013, to be printed.
3. Wu YG, Bennett J, Talla D, Stocco C. Testosterone, not 5α-dihydrotestosterone, stimulates LRH-1 leading to FSH-independent
expression of Cyp19 and P450scc in granulosa cells. Mol Endocrinol 2011 Apr; 25(4): 656-68.
4. Wu YG, Zhou P, Lan GC, Gao D, Li Q, Wei DL, Wang HL, Tan JH. MPF governs the assembly and contraction of actomyosin rings
by activating RhoA and MAPK during chemical-induced cytokinesis of goat oocytes. PLoS One 2010 13; 5(9): e12706: 1-12.
5. Wu YG, Yong Liu, Ping Zhou, Guo-Cheng Lan, Dong Han, De-Qiang Miao, Jing-He Tan. Selection of oocytes for in vitro maturation
by brilliant cresyl blue staining: a study using the mouse model. Cell Research 2007; 17(8): 722-31.
6. Wu YG, Zhou P, Lan GC, Wang G, Tan JH. The effects of delayed activation or MG132 treatment on nuclear remodeling and
development of cloned embryos depends upon the age of cytoplasts. Cloning Stem Cells 2007; 9(3): 417-431.
7. Liu N, Wu YG, Lan GC, Sui HS, Ge L, Wang JZ, Liu Y, Qiao TW, Tan JH. Pyruvate prevents aging of mouse oocyte. Reproduction
2009; 138: 223-234. (equally contributed first author)
8. Wu YG, Yu YS, Tan JH. Regualtion of foliculargenesis and development in mammals. J Zoology. 2002, 37(6)
9. Wu Y-G, Lee H-J, Barad DH, Kushnir VA, Lazzaroni-Tealdi E, Gleicher N. Effects of advancing female age on proliferation and
gene expression in cultured human granulosa cells from post-hCG retrieved follicles. Fertil Steril 2014. 101(3): e331-e332.
10. Wu Y-G, Lee HJ, Barad DH, Kushnir VA, Lazzaroni-Tealdi E, Sen A, Gleicher N. The effects of dehydroepiandrosterone (DHEA) on
cultured human granulosa cells from postovulatory human follicles after in vitro fertilization (IVF). Fertil Steril 2014. 102(3): e272
11. Wu Y, Albertini DF,Wang Q, Barad DH, Kushnir VA, Lazzaroni-Tealdi E, Gleicher N, Functional Characterization Of Cultured
Human Granulosa Cells In Serum Free Culture System. ASRM Annual Meetings, Baltimore, USA, 10/21/2015
12. Wu Y, Lazzaroni-Tealdi E, Wang Q, Albertini DF, Barad DH, Kushnir VA, Gleicher N, Randomized Comparison Of Embryo
Develoment In Closed Time-Laspse Photography System With Traditional Standard Embryology Culture With Day-3 Embryo
Transfers. ASRM Annual Meetings, Baltimore, USA, 10/21/2015.
3 of 4 13. Bennett J, Wu YG, Gossen J, Zhou P, Stocco C. Loss of GATA-6 and GATA-4 in Granulosa Cells Blocks Folliculogenesis,
Ovulation, and Follicle Stimulating Hormone Receptor Expression Leading to Female Infertility. Endocrinology. 2012 153(5):2474-85
14. Zhou P, Wu YG, Wei DL, Li Q, Wang G, Zhang J, LuoMJ, Tan JH. Mouse Cumulus-Denuded Oocytes Restore Developmental
Capacity Completely When Matured with Optimal Supplementation of Cysteamine, Cystine, and Cumulus Cells. Biol Reprod 2010;
82(4): 759-68.
15. Lan GC, Wu YG, Han D, Ge L, Liu Y, Wang HL, Wang JZ, Tan JH. Demecolcine-assisted enucleation of goat oocytes: protocol
optimization, mechanism investigation, and application to improve the developmental potential of cloned embryos. Cloning Stem
Cells 2008; 10(2): 189-202.
16. Kushnir VA, Wu Y-G, Chen I-C, Lee H-J, Barad DH, Lazzaroni-Tealdi E, Gleicher N. Mitochondrial DNA abundance declines in
human cumulus granulosa cells with age. Fertil Steril 2014. 102(3): e331
17. Lee H-J, Wu Y-G, Lazzaroni-Tealdi E, Barad DH, Kushnir VA, Gleicher N. Aging is associated with differential gene expression in
human granulosa and cumulus cells leading to rapid progression of meiotic and oocyte maturation competence. Fertil Steril 2014.
102(3): e200-e201.
18. Zhou P, Baumgarten SC, Wu Y, Bennett J, Winston N, Hirshfeld-Cytron J, Stocco C. IGF-I signaling is essential for FSH stimulation
of AKT and steroidogenic genes in granulosa cells. 2013 Mol Endocrinol Mar;27(3):511-23.
19. Qin Y, Ji H, Wu Y, Liu H. Chromosomal instability of murine adipose tissue-derived mesenchymal stem cells in long-term culture
and development of cloned embryos. Cloning Stem Cells 2009; 11(3): 445-52.
20. Zhou P, Wu YG, Li Q, Lan GC, Wang G, Gao D, Tan JH. The interactions between cysteamine, cystine and cumulus cells increase
the intracellular glutathione level and developmental capacity of goat cumulus-denuded oocytes. Reproduction 2008; 135: 605-611.
21. Lan GC, Han D, Wu YG, Han ZB, Ma SF, Liu XY, Chang ZL, Tan JH. Effects of duration, concentration, and timing of ionomycin
and 6-dimethylaminopurine (6-DMAP) treatment on activation of goat oocytes. Mol Reprod Dev 2005; 71(3): 380-388.
22. Han D, Lan GC, Wu YG, Han ZB, Wang HL, Tan JH. Factors Affecting the Efficiency and Reversibility of Roscovitine (ROS) Block
on the Meiotic Resumption of Goat Oocytes. Mol Reprod Dev. 2006; 73:238-246.
23. Han ZB, Lan GC, Wu YG, Han D, Feng WG, Wang JZ, Tan JH. Interactive Effects of Granulosa Cell Apoptosis, Follicle Size, COC
Morphology and Cumulus Expansion on the Developmental Competence of Goat Oocytes: A Study Using the Well-in-Drop (WID)
Culture System. Reproduction. 2006 132: 749-758.
24. Lan GC, Chang ZL, Luo MJ, Jiang YL, Han D, Wu YG, Han ZB, Ma SF, Tan JH. Production of Cloned Goats by Nuclear Transfer of
Cumulus Cells and Long-Term Cultured Fetal Fibroblast Cells into Abattoir-Derived Oocytes. Mol Reprod Dev 2006; 73: 834-840.
25. Han D, Zhao BT, Liu Y, Li JJ, Wu YG, Lan GC, Tan JH. Interactive effects of low temperature and roscovitine (ROS) on meiotic
resumption and developmental potential of goat oocytes. Mol Reprod Dev 2008; 75(5): 838-46.
26. Li Q, Miao DQ, Zhou P, Wu YG, Gao D, Wei DL, Cui W, Tan JH. Glucose metabolism in mouse cumulus cells prevents oocyte
aging by maintaining both energy supply and the intracellular redox potential. Biol Reprod 2011; 84(6): 1111-8.
27. Wang HL, Chang ZL, Li KL, Lian HY, Han D, Cui W, Jiao GZ, Wu YG, Luo MJ, Tan JH. Caffeine can be used for oocyte
enucleation. Cell Reprogram 2011; 13 (3):225-32.
28. Zhou JB, Wu YG, Han D, Liu LQ, Tan XW, Liu N, Luo MJ, Chang ZL, Tan JH. Effects of sperm and oocyte quality control on
intracytoplasmic sperm injection (ICSI) in goats. Shi Yan Sheng Wu Xue Bao. 2004 Oct;37(5):367-74.
29. Zhou JB, Wu YG, Liu LQ, Luo MJ, Chang ZL, Tan XW, Liu N, Tan JH. Studies on in vitro capacitation of goat spermatozoa by
heparin treatment. Sheng Wu Gong Cheng Xue Bao. 2004 Mar; 20(2):252-6.
30. Zhou JB, Wu YG, Luo MJ, Han D, Liu LQ, Chang ZL, Tan JH. The factors affect in vitro fertilization in goat. Dong Wu Xue Bao.
2004, 50(2).
31. Tan JH, Luo MJ, Wu YG. The progress in animal breeding technology. Shandong Xu Mu Shou Yi. 2008, 3.
32. Gleicher N, McAlpine JN, Gilks CB, Kushnir VA, Lee HJ, Wu YG, Lazzaroni-Tealdi E, Barad DH.
Absence of BRCA/FMR1 correlations in women with ovarian cancers. PLoS One. 2014 Jul 18;9(7)
33. Kushnir VA, Yu Y, Barad DH, Weghofer A, Himaya E, Lee H-J, Wu YG, Shohat-Tal A, Lazzaroni-Tealdi E, Gleicher N.
Utilizing FMR1 gene mutations as predictors of treatment success in human in vitro fertilization. PLoS One. 2014 Jul 14;9(7)
34. Weghofer A, Himaya E, Kushnir VA, Barad DH, Lazzaroni-Tealdi E, Yu Y, Wu Y-G, Gleicher N. Some aspects of inferactivity
between endocrine and immune systems required for successful resproduction. Reprod Biol Endocrinol 2015; 13:29.
35. Gleicher N, Yu Y, Himaya E, Barad DH, Weghofer A, Wu YG, Albertini DF, Want VQ, Kushnir VA. Early decline in functional ovarian
reserve in young women with low (CGGn<26) FMR1 gene alleles. Trans Res 2015; epub ahead of print.
36. Lee HJ, Barad DH, Kushnir VA, Shohat-Tal A, Lazzaroni-Tealdi E, Wu YG, Gleicher N. Rescue in vitro maturation (IVM) of
immature oocytes in stimulated cycles in women with low functional ovarian reserve (LFOR). Endocrine 2015; 9 (1-7).
37. Gleicher N, Vega, Darmon SK, Weghofer A, Barad DH, Wu YG, Wang Q, Zhang L, Albertini D, Kushnir VA. Live-birth rates in very
poor prognosis patients who are defined as poor responders under the Bologna criteria with nonelective single embryo, two-embryo,
and three or more embryos transferred. Fertility & Sterility 2015;epub ahead of print
4 of 4 38. Barad DH, Khanna P, Kushnir VA, Lazzaroni-Tealdi E, Wu Y-G, Lee H-J, Gleicher N. Differences in amh assessments between
standard and new high sensitivity AMH assays and impact on prediction of ivf outcomes. Fertil Steril 2014. 10:77.
Presentations list 1. Yan‐Guang Wu, Ping Zhou, Carlos Stocco. Androgen receptor‐Aryl hydrocarbon receptor Interaction Mediates Testosterone Specific Regulation of Liver Receptor Homolog‐1 expression in granulosa cells. . ISRS 2012 (Illinois symposium on reproductive science) Annual Meeting. 2. Yan‐Guang Wu and Carlos Stocco. Intracellular Crosstalk between FSH and Androgens in Granulosa Cells. SSR 2012 Annual Meeting Abstracts 362. 3. Yan‐Guang Wu and Carlos Stocco. Testosterone, not 5alpha‐dihydrotestosterone Stimulates Liver Receptor homolog‐1 Expression by Activating the Androgen Receptor in Primary Rat Granulosa Cells. SSR 2011 Annual Meeting. Abstracts 702. 4. Yan‐Guang Wu and Carlos Stocco. Specific activation of the androgen receptor by testosterone stimulates liver receptor homolog‐1 expression in rat primary granulosa cells. ISRS 2011 (Illinois symposium on reproductive science) Annual Meeting. 5. Yan‐Guang Wu and Carlos Stocco. Specific activation of the androgen receptor by testosterone stimulates liver receptor homolog‐1 expression in rat primary granulosa cells. 2011 UIC College of Medicine Research 2011. Abstract 117. 6. Yan‐Guang Wu, Jill Bennett, Deepika Talla and Carlos Stocco. Testosterone, not 5a‐dihydrotestosterone stimulates LRH‐1 leading to FSH independent expression of Cyp19 and p450scc. ISRS 2010 (Illinois symposium on reproductive science) Annual Meeting. 7. Yan‐Guang Wu, Deepika Talla, Carlos Stocco, Testosterone, not 5a‐dihydrotestosterone stimulates LRH‐1 leading to FSH independent expression of Cyp19 and p450scc in granulosa cells. UIC College of Medicine Research 2010. Abstract 134. 5 of 4