ultrafast real-time
digital autoradiography solutions
Follow acquisition in Real-Time
Acquire highly detailed images in a few hours
See the first results in minutes
Keep your research moving
real-time digital autoradiography
- All commonly used beta emitting radioisotopes :3H, 14C, 32P, 35S, 125I
- Positron emitting (PET) isotopes
- Secondary beta emissions from several gamma emitting radioisotopes: 123I, 131I, 99mTc, 201Tl, 111In.
Real-time autoradiography: distribution of D1 receptors
Key Benefits:
in rat brain striatum following tritiated ligand binding
See the first results in minutes
See data accumulating in real-time
1min
5min
15min
60min
Avoid under/over exposure
Courtesy of Janssen Research Foundation
High sensitivity & rapid image acquisition
accurate quantification
Linear dynamic range of the BetaIMAGER
Key Benefit:
Detect and quantify both high and low
sample concentrations
in a single data acquisition
Courtesy of Dr. Alain Schweitzer, Novartis
BetaIMAGERtm
tRacer
turns autoradiography into a fast and easy routine
Rat Brain sections labeled with a tritiated probe
Key Benefit:
Unmatched sensitivity cuts
12 week exposure
12 hour data acquisition
Film Autoradiogram
BetaIMAGER TRACER
your data acquisition time
from weeks to hours
Langlois and al,, 2001
A study of NK3 receptor occupancy
Key Benefit:
Detect ultra-low levels of bound
radiolabeled ligand
Use minimal amounts of radiolabeled
compound
Reduce radioactive waste
The sensitivity of the BetaIMAGER TRACER system allows visualization and quantitative analysis of tritiated senktide binding to
NK3 receptors in a matter of hours when other autoradiography
methods would have taken months. Langlois and al., 2001
Minimize experimental costs
Large sample size & high resolution in one system
QWBA rat section labeled with tritium
200 x 250 mm maximum field of view
FOV: 200 x 250 mm
Image multiple tissue sections
- up to 15 microscopy slides
FOV: 75 x 100 mm
- or a single larger sample (e.g.:TLC plate)
Optical zoom mode:
50 micron spatial resolution for smaller sample area
Courtesy of A. Molatt and P. Mitchell, Pfizer
wide range of applications
high throughput screening
receptor binding studies
whole body imaging
electrophoresis gels & blots
in situ hybridization
TLC plates
BetaIMAGERtm
dFine
the high resolution digital autoradiography Solution
For sections mounted on standard glass microscope slides
(imaging area is 24 x 32 mm)
Four position sample stage
Automated sequential data acquisition for all four samples
Spatial resolution of 10 μm can be achieved with 3H labelling
Ex vivo imaging of [11C]5 retention in 22 month old female tg/tg APP/ PS1 animals
following bolus injection of a [11C]5/[3H]1a cocktail. Yellow scale bars =100 μm.
Yousefi et al., 2010
excellent multi-label imaging capability
Multiplex various ligands and/or tracers in a single experiment
Separate and accurately discriminate between radioisotopes based on either differences
in energy (2 radioisotopes), or decay rates (up to 3 radioisotopes)
A fast and sensitive method for simultaneously determining the distribution of several
molecules in a single tissue section
Establishing the histopathology of
myocardial infarct in a rat based model
111In-DTPA
(Blue): Detects increased extracellular fluid
within fibrotic areas.
99mTc-Sestamibi
(Green): Detects both the ischemic
and viable tissue surrounding the necrosis based on
levels of tissue perfusion.
18FDG
(Red): Detects all tissue with an active glycolitic
metabolism
Single
acquisition
Isotopic
separation
Fusion
A S. Poussier and al, 2005
Possible combinations of energy based dual-detection radioisotope pairs include 3H/14C,
3H/35S, 3H/32P
or 33P and for decay rate based multi-label combinations ,99mTc/ 111In/18F.
wide range of applications
Ex vivo imaging before/after in vivo μPET/μSPECT
Co-localization of various receptors, ligands
receptor binding assays
microarrays
Technical Specifications
BetaIMAGERtm
tRacer
BetaIMAGERtm
dFine
Technology
Detection
Gaseous avalanche chamber
Scintillating foil
Camera
Intensified CCD Camera
Intensified CCD Camera
Performance
Pixel Resolution
5µm
1µm
Spatial Resolution
From 50µm to 400µm depending on isotope,
zoom and sample
10µm to 25µm depending on
isotope
Field of View (FOV)
15 microscope slides: 200mm x 250 mm
6 microscope slides: 120mm x 160 mm
2 microscope slides: 75mm x 100 mm
Half microscope slide: 25 mm x 33 mm
24 mm x 32 mm
Linearity
Linear response over a dynamic range of 104
Linear response over a dynamic range of 104
Multilabelling Capability
Energy Separation
2 isotopes chosen from 3 different families of beta emitters : low energy (3H); medium energy
(14C, 35S, 33P, 99mTc,…) and high energy (32P, 18F, 11C,…)
Radioactive Decay
Separation
Up to 3 Beta emitting isotopes (including PET) with sufficiently different radioactive decay rates
can be imaged simultaneously and then separated
Cross talk (typical)
5 % for a 3H/14C separation
1 % for a 3H/14C separation
Standard
Tritiated membranes
Non Tritiated membranes (14C, 35S, 33P,…)
15 microscope slides
4 position Automated microscope slide
changer
On request
Multi-isotopes sample holder
1 slide (127 x 178 mm) special sample holder
4 slides (120 x 170 mm) special sample holder
9 slides (51 x 76 mm) special sample holder
12 slides (38 x 76 mm) special sample holder
Sample Holder
Softwares
Image Acquisition
Beta-T Acquisition Software
Beta-D Acquisition Software
Image Analysis
M3Vision Analysis Software
M3Vision Analysis Software
Data Formats
Bitmap, JPEG, illustration & quantitative TIFFs,
ASCII
Bitmap, JPEG, illustration & quantitative TIFFs,
ASCII
Operating System
Windows XP/7
Windows XP/7
Power Consumption
Maximum: 250 W @ 230 V or 115 V
Maximum: 50 W @ 230 V or 115 V
Dimensions
75x70x160 cm (WxDxH)
40x45x75 cm (WxDxH)
Weight
145 kg
35 kg
System Requirements
FOR MORE INFORMATION
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www.biospacelab.com
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OR WRITE US:
[email protected]
BIOSPACE LAB SA, France
13, rue Georges Auric
75019 Paris France
t + 33 (0)1 44 52 88 10
f + 33 (0)1 44 52 88 39
[email protected]
BIOSPACE LAB Inc., USA
185 Alewife Parkway
#410Cambridge, MA
02138, USA
t + 1 201 785 4032
[email protected]