High-Performance Liquid Chromatography
ULTRON
Enantiomer Separation Columns
ES Series
ES-OVM
ES-PEPSIN
ES-CD
ES-PhCD
ULTRON ES-OVM
Employs the protein ovomucoid as the stationary phase, enabling separations of a wide variety of compounds
ranging from basic to acidic. Displays high levels of resistance to denaturation, and can be used over wide solvent
concentration and temperature ranges.
Ketoprofen
Citalopram oxalate
Tolperisone hydrochloride
・HCl
O
NC
O
CH3
O
OH
O
OH
O
CH3
N
N
CH3
HO
CH3
H3C
O
F
0
10
20
0
30
Column: ULTRON ES-OVM (5 µm)
Column size: 150 mm x 4.6 mm I.D.
Mobile phase: 20 mM KH2PO4 + 20 mM K2HPO4
(pH 5.5)/C2H5OH = 95/5
Flow rate: 1.0 mL/min.
Column temp.: 25℃
Detection: UV-257 nm
Injection vol.: 2 µL
10
20
0
30
Column: ULTRON ES-OVM (5 µm)
Column size: 150 mm x 4.6 mm I.D.
Mobile phase: 50 mM KH2PO4 + 0.2 N NaOH
(pH 7.0)/CH3CN = 17/3
Flow rate: 0.6 mL/min.
Column temp.: 30℃
Detection: UV-240 nm
Injection vol.: 15 µL
10
20
30
Column: ULTRON ES-OVM (5 µm)
Column size: 150 mm x 4.6 mm I.D.
Mobile phase: 20 mM KH2PO4 + H3PO4 (pH 3.0)
/CH3CN = 90/10
Flow rate: 1.0 mL/min.
Column temp.: 25℃
Detection: UV-254 nm
Injection vol.: 2 µL
ULTRON ES-PEPSIN
Employs the digestive enzyme pepsin, which is chemically bonded to the base material in an extremely stable
manner. Displays superior recognition with respect to basic compounds such as aminoalcohols, as well as excellent
stability during analyses.
Alprenolol
Atenolol
Bunitrolol
NH2
OH
CH3
CH3
H
N
O
CH3
CH3
O
H3C
CH3
N
H
O
O
N
H
N
OH
CH3
OH
CH2
Column: ULTRON ES-PEPSIN (5 µm)
Column size: 150 mm x 4.6 mm I.D.
Mobile phase: 20 mM KH2PO4 (pH 5.0)/C2H5OH
= 95/5
Flow rate: 1.0 mL/min
Column temp.: 25℃
Detection: UV-220 nm
Column: ULTRON ES-PEPSIN (5 µm)
Size: 150 mm x 4.6 mm I.D.
Mobile phase: 20 mM KH2PO4 (pH 6.0)/C2H5OH
= 99/1
Flow rate: 1.0 mL/min
Column temp.: 25℃
Detection: UV-220 nm
Column: ULTRON ES-PEPSIN (5 µm)
Size: 150 mm x 4.6 mm I.D.
Mobile phase: 20 mM KH2PO4 (pH 5.0)/C2H5OH
= 95/5
Flow rate: 1.0 mL/min
Column temp.: 25℃
Detection: UV-220 nm
ULTRON ES-CD / ES-PhCD
ULTRON ES-CD and ES-PhCD columns are chiral separation columns employing chemically bonded betacyclodextrin (CD) and phenylated beta-cyclodextrin (PhCD) as stationary phases.
・Capable of chiral separations of hydrophobic cyclic compounds.
・Both reverse and normal phase eluents can be used.
・Chiral separations of a wide range of compounds are possible using these two types of columns.
・Display superior stability and durability.
Valsartan
Terbutaline sulfate
H
O
CH3
N
H
N
HO
N
CH3
OH
OH
NH
Cyclobenzaprine hydrochloride
N
H3 C
H2SO4
CH3
H3C
N
N
CH3
HCl
H
CH3
OH
O
2
CH3
0
10
20
0
30
10
20
0
30
Column: ULTRON ES-CD (5 µm)
Column size: 150 mm x 6.0 mm I.D.
Mobile phase: 20 mM KH2PO4 + 20 mM K2HPO4
(pH 6.0)/CH3CN = 95/5
Flow rate: 0.7 mL/min.
Column temp.: 25℃
Detection：: UV-220 nm
Injection vol.: 2 µL
Column: ULTRON ES-CD (5 µm)
Column size: 150 mm x 6.0 mm I.D.
Mobile phase: 20 mM KH2PO4 (pH 4.6)/CH3CN
= 90/10
Flow rate: 1.0 mL/min.
Column temp.: 25℃
Detection: UV-225 nm
Injection vol.: 2 µL
Propranolol hydrochloride
10
20
30
Column: ULTRON ES-CD (5 µm)
Column size: 150 mm x 6.0 mm I.D.
Mobile phase: 20 mM KH2PO4 + H3PO4 (pH 3.0)
/CH3CN = 70/30
Flow rate: 1.0 mL/min.
Column temp.: 25℃
Detection: UV-290 nm
Injection vol.: 2 µL
Duloxetine hydrochloride
Rabeprazole sodium
CH3
HO
N
H
N
CH3
CH3
H
N
O
O
N
Na
O
HCl
OCH3
CH3
S
O
・HCl
N
S
0
5
10
15
20
Column: ULTRON ES-PhCD (5 µm)
Column size: 150 mm x 6.0 mm I.D.
Mobile phase: 20 mM KH2PO4 (pH 4.6)/CH3CN
= 60/40
Flow rate: 1.0 mL/min.
Column temp.: 25℃
Detection: UV-220 nm
Injection vol.: 2 µL
0
10
20
Column： ULTRON ES-PhCD (5 µm)
Column size: 150 mm x 6.0 mm I.D.
Mobile phase: 20 mM KH2PO4 (pH 4.6)
/CH3CN = 60/40
Flow rate: 1.0 mL/min.
Column temp.: 25℃
Detection: UV-292 nm
Injection vol.: 2 µL
30
0
10
20
Column: ULTRON ES-PhCD (5 µm)
Column size: 150 mm x 6.0 mm I.D.
Mobile phase: 20 mM KH2PO4 + H3PO4 (pH 3.0)
/CH3CN = 65/35
Flow rate: 1.0 mL/min.
Column temp.: 25℃
Detection: UV-290 nm
Injection vol.: 2 µL
ULTRON ES Series Specifications
Product name
ULTRON ES-OVM
ULTRON ES-OVM
ULTRON ES-OVM
ULTRON ES-OVM
ULTRON ES-OVM Guard column
ULTRON ES-OVM-C (tested using clopidogrel bisulfate)
ULTRON ES-OVM.Prep
ULTRON ES-OVM.Prep Guard column
ULTRON ES-OVM Guard cartridges (x2)
ULTRON ES-OVM Guard cartridges (x2)
Guard cartridge holder (with adaptor)
Guard cartridge holder (with adaptor)
ULTRON ES-PEPSIN
ULTRON ES-PEPSIN Guard column
Particle size (µm)
Dimensions (mm)
5
150 × 2.0
5
150 × 4.6
5
150 × 6.0
10
250 × 4.6
5
10 × 4.0
5
150 × 4.6
10
250 × 20.0
10
15 × 8.0
5
5 × 2.0
5
10 × 4.6
For 5 × 2.0 mm columns
For 10 × 4.6 mm columns
5
5
USP column
specification
L57
L57
L57
L57
150 × 4.6
10 × 4.0
ULTRON ES-CD
ULTRON ES-CD
ULTRON ES-CD Guard column
ULTRON ES-CD Guard cartridges (x2)
ULTRON ES-CD Guard cartridges (x2)
Guard cartridge holder (with adaptor)
Guard cartridge holder (with adaptor)
5
150 × 2.0
5
150 × 6.0
5
10 × 4.0
5
5 × 2.0
5
10 × 4.6
For 5 × 2.0 mm columns
For 10 × 4.6 mm columns
L45
L45
ULTRON ES-PhCD
ULTRON ES-PhCD
ULTRON ES-PhCD Guard column
ULTRON ES-PhCD Guard cartridges (x2)
ULTRON ES-PhCD Guard cartridges (x2)
Guard cartridge holder (with adaptor)
Guard cartridge holder (with adaptor)
5
150 × 2.0
5
150 × 6.0
5
10 × 4.0
5
5 × 2.0
5
10 × 4.6
For 5 × 2.0 mm columns
For 10 × 4.6 mm columns
L45
L45
●Protecting columns using guard columns
By using guard columns together with analytical columns, analytical columns can be protected from contamination, enabling
longer column lifetimes. The use of guard columns is particularly recommended when analyzing samples containing large
amounts of impurities. Guard cartridges are also available when guard columns are changed frequently. Please use guard
columns with the same or smaller inner diameter than that of the analytical column being used.
●ULTRON ES-OVM-C
Clopidogrel bisulfate evaluation conditions based on USP
standards employed for shipping inspections.
(Performance reports included with columns.)
Please feel free to contact us with questions related to analyses. Column screening services are also available.
Please be aware that specifications are subject to change without prior notification.
Contact details for inquiries
Shinwa Chemical Industries Ltd.
50-2 Kagekatsu-cho, Fushimi-ku
Kyoto 612-8307 Japan
TEL: +81-75-621-2360 FAX: +81-75-602-2660
E-mail: [email protected]
Website: http://shinwa-cpc.co.jp/en/
SA1016A-E