John Strouboulis
CURRICULUM VITAE
1. Personal Details
Name:
Date of Birth:
Ctizenship:
John Strouboulis
13 October 1964
Greek and US dual citizenship
Present address:
Institute of Molecular Biology and Biotechnology
Foundation of Research & Technology Hellas
100 Nikolaou Plastira Street
GR-70013 Heraklion, Crete
Greece
Tel: +30-2810-391230
Fax: +30-2810-391101
URL: www.imbb.forth.gr
e-mail: [email protected]
2. Education
1989-1994:
1988-1989:
1984-1987:
Ph.D. in Molecular Biology, MRC National Institute for Medical Research,
London, UK
Regulation of the complete human -globin locus in transgenic mice.
Supervisor: Dr. Frank Grosveld
M.Sc. in Molecular Genetics, University of Leicester, Leicester, UK
B.Sc. (Hons) in Biochemistry and Applied Molecular Biology, University
of Manchester, Institute of Science and Technology (UMIST), Manchester,
UK
3. Research and Professional Experience
02/2015-present:
2006-2015:
1998-2006:
1994-1998:
1987-1988:
Research Professor, Head of Proteomics
Institute of Molecular Biology and Biotechnology
Foundation of Research & Technology Hellas
Heraklion, Crete, Greece.
Proteomic and genomic characterization of transcriptional and epigenetic
regulation of hematopoiesis; molecular basis of hematopoietic disorders and
leukaemia.
Research Professor (3/2014-01/2015)
Associate Research Professor (04/2006-03/2014)
Institute of Molecular Oncology
Biomedical Sciences Research Center “Alexander Fleming”, Athens,
Greece.
Proteomic and genomic characterization of transcriptional and epigenetic
regulation of hematopoiesis
Assistant Professor (2002-06)
Staff scientist (2000-01)
Postdoctoral scientist (1998-2000)
Dept. of Cell Biology, Erasmus University Medical Center, Rotterdam,
the Netherlands.
Hematopoietic transcription factors and chromatin structure, human globin
gene regulation, Xenopus genomics
Postdoctoral Fellow
Lab. of Molecular Embryology, National Institute of Child Health and
Human Development, National Institutes of Health (NIH), Bethesda, MD,
USA. Lab Head: Dr. Alan P. Wolffe (deceased).
Isolation and characterization of X. laevis homologs of epigenetic modofiers,
Polycomb, HP1 and MeCP1
Research technician
Lab. of Molecular Oncology, National Cancer Institute, NIH, Frederick,
MD, USA. Lab Chief: Dr. Takis S. Papas (deceased).
Differential expression of ets-related oncogenes in myeloid and lymphoid
leukemias.
4. Grants and Awards
1
John Strouboulis
2015-2018:
H2020 Marie Curie ETN Chromatin3D: Chromatin Dynamics in
Development and Disease. Project: The role of Friend of GATA-1 (FOG-1)
in chromatin looped domains. JS lab budget: € 242,387.
Fondazione Cariplo (Italian charity): Project: Strategies to increase
Hemoglobin F in β Thalassemia: the network of molecular interactions of the
γ-globin repressors Sox6, CoupTF2 and Bcl11a. JS lab budget: €60,000.
FP7 Marie Curie Initial Training Network Hem-ID: HEMatopoietic cell
IDentity: genetic and epigenetic regulation in normal and malignant
hematopoiesis. JS lab budget: € 219,400.
NIDDK-NIH RO1 grant (Co-PI with Dr. Jörg Bungert, University of
Florida). Project: Functional Proteomics in differentiating erythroid cells. JS
lab budget: $ 555,000.
Greek Secretariat General for Research and Development, Bilateral
Greek-French collaborative grant. Project: Epigenetic regulation of
erythroid cell differentiation: the role of histone variants. JS lab budget: €
20,000.
FP7 Marie Curie Initial Training Network DisChrom: Chromatin Diseases:
from basic mechanisms to therapy. JS lab budget: € 238,400.
Coordinator, FP7 Marie Curie Initial Training Network InteGeR:
Integrated Gene Regulation: from DNA Sequence to Nuclear Structure. Total
budget coordinated by JS: €2.3 million, JS lab budget: € 308,156.
Start-up funding, BSRC Al. Fleming
€100,000
Wellcome Trust Senior Research Fellowship in Basic Sciences (declined
in favour of joining BSRC “Al. Fleming” in Greece). Project: Characterisation
of haematopoietic transcription factor complexes and gene target networks.
Budget awarded to JS: £1.55 million.
ZonMW-NWO Program Grant (Dutch Research Organization-Medical
Sciences). Project: The molecular events leading to globin gene
transcriptional activation. JS lab budget: € 500,000.
ZonMW-NWO Program Grant (Dutch Research Organization-Medical
Sciences). Project: Purification and characterization of GATA-1 related
transcription factor complexes and target genes by in vivo biotinylation
tagging. JS lab budget: € 500,000.
Innovation Orientation Program Grant (Dutch Ministry of Financial Affairs).
Functional genomics in Xenopus: a new perspective for medical research
(part of a Dutch research consortium). JS lab budget: € 128,357.
2013-2015:
2011-2015:
2010-2014:
2010-2013:
2009-2013:
2009-2012:
2006-2008:
2005:
2004-2008:
2004-2008:
2001-2005:
5. Fellowships and Studentships
FP4 Marie Curie Postdoctoral Fellowship for Research Training in
Biotechnology
NIH Intramural Research Training Award
Human Frontiers Science Programme Organisation (HFSPO) Long-Term
Postdoctoral Fellowship
EMBO Postdoctoral Fellowship
MRC Research Studentship
European Social Fund Award for Training in Biotechnology
1998-2000:
1996-1998:
1994-1996:
1994:
1990-1994:
1988-1989:
6. PhD students
Name (Nationality)
Giorgio Lucio
Papadopoulos
(Greek-Italian)
Year of award-University
July 2015
Dept. of Biology
University of Crete
Dimitris
Papageorgiou
(Greek)
June 2013
Dept. of Chemistry
University of Athens
Elena Karkoulia
(Greek-Swiss)
May 2013
Medical School
University of Crete
Katarzyna Kolodziej
(Polish)
2008
Erasmus University
Thesis title
Transcriptional and epigenetic
mechanisms of gene regulation
in cellular differentiation: the
hematopoietic paradigm
A proteomic approach in the
analysis of erythroid
transcription factors
Present position
Postdoc, Strouboulis lab
Characterization of the
molecular functions of the
transcription factor GATA-1 in
hematopoiesis
Characterization of transcription
factor complexes involved in
Postdoc, Strouboulis lab
2
Postdoc, Strouboulis lab
QC analyst, TEVA
Pharmaceuticals, the
John Strouboulis
Patrick Rodriguez
(French)
Rotterdam, Netherlands
2006
Erasmus University
Rotterdam, Netherlands
Eleni Katsantoni
(Greek)
2002
University of Crete,
Greece
Mariken de Krom
(Dutch)
2002
Erasmus University
Rotterdam, Netherlands
globin gene regulation
Characterization of
hematopoietic transcription
factor complexes in erythroid
cells
Novel cis-acting regulatory
elements in the human β-globin
locus implicated in hemoglobin
switching
Activation, regulation and
transcription of the human and
murine globin Loci
Netherlands
Staff scientist, ISREC,
Lausanne, Switzerland
Group leader, Bioacademy,
Athens, Greece
Coordinator of Rudolf Magnus
Graduate School, Utrecht
University, Netherlands
7. Teaching experience
2015:
2015:
1999-2005:
2004:
2001- 2003:
2009-2012:
Visiting lecture, DIMET graduate program in Molecular Medicine, University
of Milano-Bicocca, Italy
Lecturer, Master program in Molecular Biology and Biomedicine, Department
of Biology, University of Crete, Greece
Lecturer, Masters Program in Molecular Medicine, Erasmus University
Medical Center, Rotterdam, the Netherlands.
Lecturer in the Proteomics Technology graduate lecture series, Utrecht
University, the Netherlands.
Visiting instructor responsible for Recombinant DNA Technology laboratory
course for first year medical students, Biology Dept., Athens Medical School.
Coordinator, InteGeR FP7 Marie Curie Initial Training Network. As
coordinator I was responsible for the design and implementation of the
training activities of this EU-funded research training network which included
the organization of several scientific workshops.
8. Other information
2012- 2013:
October 2016:
May 2014:
November 2012:
October 2011:
December 2007:
2006-Present:
05/2005-01/2015:
Expert evaluator, FP7 Marie Curie Actions (Initial Training Networks,
Individual Fellowships), Research Executive Agency, European Commission,
European Union, Brussels, Belgium.
Main organizer, FEBS Workshop on Chromatin Proteomics, Heraklion,
Crete, Greece, October 3-8, 2016.
Co-organizer, FEBS/EMBO Lecture Course on Nuclear Proteomics, Kos,
Greece, May 17-22, 2014.
http://www.nuclearproteomics.org
Main organizer, Conference on Gene Regulation: from DNA Sequence
to Nuclear Structure, Athens, Greece, November 14-15, 2012.
http://www.integer-itn.eu/conference/
Co-organizer, Workshop on Functional and Structural Proteomics,
Athens, Greece, October 607, 2011. http://www.funspa2011.gr/
th
Organizing committee, 59 Conference of the Hellenic Society of
Biochemistry and Molecular Biology, Athens, Greece.
Co-founder and shareholder, BioMedCode Hellas SA, Pre-Clinical Drug
Evaluation Services (www.biomedcode.com)
Founding Director, Technology Transfer Office, BSRC Alexander
Fleming (http://www.fleming.gr/techtransfer/)
Ad-hoc grant reviewer for the European Commission (FP7), The Wellcome Trust (UK), DRG
(Germany), The Royal Society (UK), NHS Blood and Transplant (UK), NSERC (Canada), GRF (Hong
Kong), NWO (the Netherlands), ANR (France), COST Actions (EU and ESF), Greek State Scholarship
Foundation (IKY).
Reviewer: Genes and Development, EMBO J., PNAS, Blood, Genome Research, Cell Cycle, PLoS
One, BMC journals, BBA, Stem Cells, Nucleic Acids Research, Experimental Hematology, Analytical
Chemistry, Hematologica, FASEB J., DNA and Cell Biology, British Journal of Haematology, Journal of
Proteome Research.
9. Oral presentations at Conferences
2014:
th
19 Hemoglobin Switching Conference, Oxford UK, September 2014.
3
John Strouboulis
International Symposium on Gene Regulation in Normal and Pathologic
States, Prague, April 2014.
35th Conference of the Hellenic Society of Biological Sciences, Nafplion, May
2013.
International Symposium on Gene Regulation in Normal and Pathologic
States, Prague, March 2013.
1st Workshop on Chromatin Proteomics, Munich, March 2010.
2nd Immunology Summer School and Conference, Kefalonia, August 2009.
33rd FEBS Congress & 11th IUBMB Conference, Athens, June 2008.
CancerNet Workshop on Cancer Research, Molecular Cellular and
Therapeutic Aspects of Cancer, AUTH, Thesaloniki, June 2007.
th
12 European Hematology Association Congress, VIenna, June 2007.
11th European Hematology Association Congress, Amsterdam, June 2006.
Interdisciplinary workshop on “Noise and robustness in transcriptional
regulatory networks”, Coquelle, France, September 2005.
th
46 American Society of Hematology Annual Meeting, San Diego, USA.
th
14 Conference on Hemoglobin Switching, Seattle, USA.
Frontiers of Preteomics: Aims and Perspectives, Osaka, Japan.
7th Molecular Haematology Meeting, London, UK.
NHLBI RFA Meeting on hemoglobinopathies and globin gene regulation,
Bethesda, USA.
th
13 Conference on Hemoglobin Switching, Oxford, UK.
“Red Cell” meeting, Oxford, UK.
Developmental Biology and Genetics Workshop, London, UK.
“Red Cell” meeting, London, UK.
2014:
2013:
2013:
2010:
2009:
2008:
2007:
2007:
2006:
2005:
2004:
2004:
2004:
2004:
2004:
2002:
1992:
1991:
1991:
10. Invited seminars and lectures
Ottawa Hospital Research Institute, Canada, September 2015.
Genome Quebec Innovaiton Cetner, McGill University, Montreal, Canada,
September 2015.
Maisonneuve-Rosemont Hospital Research Centre, University of Montreal,
Canada, September 2015.
First Faculty of Medicine, Charles University, Prague, Czech Republic,
October 2014.
Medical School, University of Patras, March 2013.
Dept. of Biochemistry and Molecular Biology, University of Florida,
Gainesville, USA, September 2012.
Institut Albert Bonniot, Grenoble, June 2009.
Instute of Biology and Biotechnology, Hellenic National Research
Foundation, Athens, February 2008.
Netherlands Cancer Institute (NKI), Amsterdam, June 2006.
Institute of Molecular Biology and Biotechnology (ΙΜΒΒ), Crete, March 2006.
Molecular Biology Unit, Adolf Butendant Institute, Ludwig Maximilians
University, Munich, July 2005.
Netherlands National Proteomics Center Inaugural Conference, Utrecht,
February 2005.
Institute of Stem Cell Research, University of Edinburgh, January 2005.
Athens University Medical School, May 2004.
Molecular Haematology and Cancer Biology Unit, Institute of Child Health,
Great Ormond Street Hospital for Children and University College London.
Institute for Biological Investigations, Madrid.
Molecular Haematology and Cancer Biology Unit, Great Ormond Street
Hospital for Children and University College London, London.
Institute of Biology NCSR Dimokritos, Athens.
Netherlands Cancer Institute (NKI), Amsterdam.
Biochemistry Department, Oxford University, UK.
German Cancer Research Center (DKFZ), Heidelberg.
2015:
2015:
2015:
2014:
2013:
2012:
2009:
2008:
2006:
2006:
2005:
2005:
2005:
2004:
2003:
2003:
2001:
2000:
1994:
1994:
1993:
11. List of Publications appearing in PubMed (in chronological order)
Total Citations:
h-index:
1.
4079
22
Samuel, K.P., Ascione, R., Kottarides, S.D., Seth, A.K., Lautenberger, J.A., Zuber, M.,
Strouboulis, J. and Papas, T.S. Expression of animal and human retroviral gene products in
4
John Strouboulis
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
Escherichia coli with the lambda pJL6 vector system. Genet. Anal. Tech. Appl., 1990, 7, pp.
178-208.
Strouboulis, J., Dillon, N. and Grosveld, F. Developmental regulation of the complete 70kb
human beta-globin locus in transgenic mice. Gene Dev., 1992, 6, pp. 1857-1864.
Strouboulis, J., Dillon, N. and Grosveld, F. Efficient joining of large DNA fragments for
transgenesis. Nucl. Acids Res., 1992, 20, pp. 6109-6110.
Müller, A.M., Medvinsky, A., Strouboulis, J., Grosveld, F. and Dzierzak, E. Development of
hematopoietic stem cell activity in the mouse embryo. Immunity, 1994, 1, pp. 291-301.
Milot, E., Strouboulis, J., Trimborn, T., Wijgerde, M., de Boer, E., Langeveld, A., Tan-Un, K.,
Vergeer, W., Yannoutsos, N., Grosveld, F. and Fraser, P. Heterochromatin effects on the
frequency and duration of LCR-mediated gene transcription. Cell, 1996, 87, pp. 105-114.
Strouboulis, J. and Wolffe, A.P. Functional compartmentalization of the nucleus. J. Cell Sci.,
1996, 109, pp. 1991-2000.
Dillon, N., Trimborn, T., Strouboulis, J., Fraser, P. and Grosveld, F. The effect of distance on
long range chromatin interactions. Mol. Cell, 1997, 1, pp. 131-139.
Jones, P., Veenstra, G., Wade, P., Vermaak, D., Kass, S., Landsberger, N., Strouboulis, J. and
Wolffe, A.P. Xenopus MeCP2 exists in a complex with Sin3 and histone deacetylase linking
transcriptional repression on methylated DNA to chromatin modification. Nature Genetics,
1998, 19, pp. 187-191.
Strouboulis, J., Damjanowski, S., Vermaak, D., Meric, F. and Wolffe, A.P. Transcriptional
repression by XPc1, a new Polycomb homolog in Xenopus laevis embryos, is independent of
histone deacetylase. Mol. Cell. Biol., 1999, 19, pp. 3958-3968.
de Krom, M., van de Corput, M., Grosveld, F. and Strouboulis, J. Stochastic patterns in globin
gene expression are established prior to transcriptional activation and are clonally inherited.
Mol. Cell, 2002, 9, pp. 1319-1326.
de Boer, Ε., Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni, E., Heck, A., Grosveld, F. and
Strouboulis, J. Efficient biotinylation and single-step purification of tagged transcription factors
in mammalian cells and transgenic mice. Proceedings Nat. Acad. Sci. USA, 2003, 100, pp.
7480-7485.
Katsantoni, E., Langeveld, A., Wai, A., Drabek, D., Grosveld, F., Anagnou, N.P. and
Strouboulis, J. The HPFH-2 and HPFH-6 breakpoint sequences, but not HPFH-3, lead to
persistent -globin expression in adult single copy transgenic mice. Blood, 2003, 102, pp.
3412-3419.
Katsantoni, E., de Krom, M., Imam, A. Kong-a-San, J., Grosveld, F., Anagnou, N.P. and
Strouboulis, J. An embryonic-specific repressor element located 3’ to the A-globin gene
influences expression of the human -globin locus in the embryonic stage. Exp Hematol,
2004, 32, pp.224-233.
Patrinos, P., de Krom, M., de Boer, E., Langeveld, A., Imam, A., Strouboulis, J., de Laat, W.
and Grosveld, F. Multiple interactions between regulatory regions are required to stabilize an
Active Chromatin Hub. Gene Dev., 2004, 18, pp. 1495-509.
Rodriguez, P., Bonte, E., Krijgsveld, J., Guyot, B., Heck, A., Vyas, P., de Boer, E., Grosveld, F.
and Strouboulis, J. GATA-1 forms distinct activating and repressive complexes in erythroid
cells. EMBO J., 2005, 24, pp. 2354–2366.
Driegen, S., Ferreira, R., van Zon, A., Strouboulis, J., Jaegle, M., Grosveld, F., Philipsen, S.
and Meijer, D. A generic tool for biotinylation of tagged proteins in transgenic mice. Transgenic
Res., 2005, 14, pp. 477-482.
Grosveld, F., Rodriguez, P., Meier, N., Krpic, S., Pourfarzad, F., Papadopoulos, P., Kolodziej,
K.E., Patrinos, G., Hostert, A. and Strouboulis, J. Isolation and characterization of
hematopoietic transcription factor complexes by in vivo biotinylation tagging and mass
spectrometry. Ann N Y Acad Sci. 2005, 1054, pp. 55-67.
Schuh, A., Clark, A., Hamlett, I., Iborra, F., Strouboulis, J., Vyas, P. and Porcher, C. The
oncoprotein SCL/Tal-1 associates with co-repressor ETO-2 to form different complexes in
erythroid cells and megakaryocytes. Mol Cell Biol, 2005, 23, pp. 10235-50-3968.
Goardon, N., Lambert, J., Rodriguez, P., Herblot, S., Thibault, P., Dumenil, D., Strouboulis, J.,
Romeo, P-H and Hoang, T. ETO2 coordinates cellular proliferation and differentiation during
erythropoiesis. EMBO J., 2006, 25, pp. 357-366.
Meier, N., Krpic, S., Rodriguez, P., Strouboulis, J., Monti, M., Krijgsveld, J., Gering, M., Patient,
R., Hostert, A. and Grosveld, F. “Novel binding partners of Ldb1 are required for hematopoietic
development”. Development, 2006, 133(24), pp. 4913-23.
Rodriguez, P., Braun, H., Kolodziej, K.E., de Boer, E., Campbell, J., Bonte, E., Grosveld, F.,
Philipsen, S. and Strouboulis, J. Isolation of transcription factor complexes by in vivo
biotinylation tagging and direct binding to streptavidin beads. Methods Mol Biol., 2006, 338,
pp. 305-23.
Horsman, S., Moorhouse, M., de Jager, V., van der Spek, P., Grosveld, F., Strouboulis, J. and
Katsantoni, E. “TFT Mapper: A BLAST search tool for identification of Transcription Factors
Target Genes”. BMC Bioinformatics, 2006, 7, pp. 120.
5
John Strouboulis
23. Sanchez, C., Sanchez, I., Demmers, J.A., Rodriguez, P., Strouboulis, J. and Vidal M.
Proteomic analysis of Ring1B/Rnf2 interactors identifies a novel complex with the Fbxl10/
Jmjd1B histone demethylase and the BcoR corepressor. Mol Cell Proteomics, 2007, 6(5), pp.
820-834.
24. Moerland, M., Anghelescu, N., Samyn, H., van Haperen, R., van Gent, T., Strouboulis, J., van
Tol. A., Grosveld, F. and de Crom R. Inducible expression of phospholipid transfer protein
(PLTP) in transgenic mice: acute effects of PLTP on lipoprotein metabolism. Transgenic Res.,
2007, 16(4), pp. 503-13.
25. Katsantoni, E.Z., Anghelescu, N.E., Rottier, R., Moerland, M., Antoniou, M., de Crom, R.,
Grosveld, F. and Strouboulis, J., Ubiquitous expression of the rtTA2S-M2 inducible system in
transgenic mice driven by the human hnRNPA2B1/CBX3 CpG island. BMC Dev Biol., 2007, 7,
pp. 108.
26. Liang, S., Moghimi, B., Yang, T.P., Strouboulis, J. and Bungert, J. Locus control region
mediated regulation of adult β-globin gene expression. J Cell Biochem., 2008, 105(1), pp. 916.
27. Hamlett, I., Draper, J., Strouboulis, J., Iborra, F., Porcher. C. and Vyas, P. Characterization of
megakaryocyte GATA1-interacting proteins: the corepressor ETO2 and GATA1 interact to
regulate terminal megakaryocyte maturation. Blood, 2008. 112(7), pp. 2738-49.
28. Kolodziej, K.E., Pourfarzad, F., de Boer, E., Krpic, S., Grosveld, F. and Strouboulis J. Optimal
use of tandem biotin and V5 tags in ChIP assays. BMC Mol Biol, 2009, 5, pp. 6.
29. Tsiftsoglou, A.S., Vizirianakis, I.S. and Strouboulis, J. Erythropoiesis: Model Systems,
Molecular Regulators, and Developmental Programs. IUBMB Life, 2009, 61(8), pp. 800-30.
30. Liang, S.Y., Moghimi, B., Crusselle-Davis, V.J., Lin, I.J., Rosenberg, M.H., Li, X., Strouboulis J,
Huang, S. and Bungert, J. Defective erythropoiesis in transgenic mice expressing dominantnegative upstream stimulatory factor. Mol Cell Biol, 2009, 29(21), pp. 5900-10.
31. Anantharaman, A., Lin, I.J., Barrow, J., Masannat, J., Strouboulis, J., Huang, S. Bungert, J.
The Role of Helix-Loop-Helix Proteins During Differentiation of Erythroid Cells. Mol Cell Biol,
2011, 31(7), pp. 1332-43.
32. Cui, S., Kolodziej, K., Obara, N., Amaral-Psarris, A., Demmers, J., Shi, L., Engel, J.D.,
Grosveld, F., Strouboulis, J.* and Tanabe, O. Nuclear Receptors TR2 and TR4 Recruit Multiple
Epigenetic Transcriptional Corepressors That Associate Specifically with the Embryonic β-Type
Globin Promoters in Differentiated Adult Erythroid Cell. Mol Cell Biol, 2011, 31(16), pp.298311. * Co-corresponding author.
33. Leotoing, L., Chereau, F., Baron, S., Hube, F., Valencia, H.J., Bordereaux, D., Demmers, J.A.,
Strouboulis, J. and Baud, V. ABIN-2 is an activator of IKKα-mediated NF-κB transcriptional
activity. J Biol Chem, 2011, 286(37), pp. 32277-88.
34. Reynolds, N., Latos, P., Hynes-Allen, A., Leaford, D., O’Shaughnessy, A., Mosaku, O.,
Signolet, R., Brennecke, P., Kalkan, T., Costello, I., Humphreys, P., Mansfield, W., Nagakawa,
K., Strouboulis, J., Behrens, A., Bertone, P. and Hendrich, B. NuRD enforced transcriptional
heterogeneity enables lineage commitment of embryonic stem cells. Cell Stem Cell, 2012
10(5), pp. 583-94.
35. Brazão, T.F., Demmers, J., van IJcken, W., Strouboulis, J., Fornerod, M., Romão, L and
Grosveld, F.G. ROD1 is Involved in Nonsense-Mediated mRNA Decay. FEBS Lett, 2012
586(8), pp. 1101-10.
36. Stees, J.S., Varnf, F., Huang, S., Strouboulis, J., and Bungert, J. Recruitment of Transcription
Complexes to Enhancers and the Role of Enhancer Transcription. Biology, 2012, 1, pp. 778793.
37. Papadopoulos, G.L., Karkoulia, E., Tsamardinos, I., Porcher, C., Ragoussis, J., Bungert, J.,
and Strouboulis J. GATA-1 genome-wide occupancy associates with distinct epigenetic
profiles during mouse fetal liver erythropoiesis. Nucleic Acids Res., 2013, 41(9), pp. 4938-48.
38. Papageorgiou, D.N., Demmers, J. and Strouboulis, J. The use of NP-40 significantly reduces
contamination of nuclear extracts by endogenously biotinylated carboxylases in the affinity
purification of biotin-tagged nuclear factors. Protein Expr Purif., 2013, 89, pp. 80-83.
39. Shi, L., Sierant, M.C., Gurdziel, K., Zhu, F., Cui, S., Kolodziej, K.E., Strouboulis, J., Guan, Y.,
Tanabe, O., Lim, K-C. and Engel, J.D. Biased, non-equivalent gene-proximal and -distal motifs
of orphan nuclear receptor TR4 in human primary erythroid cells. PLoS Genetics, 2014,
10:e1004339.
40. Fan, J.A.X., Papadopoulos, G.L., Lin, I-J., Hossain, M., Hu, J., Renne, R., Strouboulis, J.* and
Bungert, J. Proteomic and Genomic Analysis of transcription factor TFII-I in erythroleukemia
cells. Nucleic Acids Res., 2014, 42, pp. 7625-41. * Co-corresponding author.
41. Papadopoulos, P., Gutiérrez, L., Demmers, J., Scheer, E., Pourfarzad, F., Papageorgiou, D.N.,
Karkoulia, E., Strouboulis, J., van de Werken, H.J., van der Linden, R., Vandenberghe, P.,
Dekkers, D.H., Philipsen, S., Grosveld, F. and Tora L. TAF10 Interacts with the GATA1
Transcription Factor and Controls Mouse Erythropoiesis. Mol Cell Biol, 2015, 35, pp. 2103-18.
42. Blobel, G., Bodine, D., Brand, M., Crispino, J., De Bruijn, M., Nathan, D., Pappayannopoulou,
T., Porcher, C., Strouboulis, J., Zon, L., Higgs, D.R., Stamatoyannopoulos, G. and Engel J.D.
6
John Strouboulis
An International Effort to Cure a Global Health Problem: A report on the 19th Hemoglobin
Switching Conference. Exp Hematol, 2015, in press.
43. Amanatiadou E.P, Papadopoulos, G.L., Strouboulis, J.* and Vizirianakis I.S.* GATA1 and PU.1
bind to ribosomal protein genes in erythroid cells: implications for ribosomopathies. * Cocorresponding authors. PLoS One, in press.
44. Burda, P., Vargova, J., Curik, N., Papadopoulos, G.L., Strouboulis, J. and Stopka T. GATA-1
represses the PU.1 gene by recruiting DNMT1 and creating a repressive chromatin structure.
Submitted to Oncogenesis.
12. Book Chapters and Published Conference Proceedings
1.
Grosveld, F., Greaves, D., Philipsen, S., Pruzina, S., de Boer, E., Hanscombe, O., Belhumeur,
P., Hurst, J., Fraser, P., Whyatt, D., Antoniou, M., Mignotte, V., Dillon, N., Lindenbaum, M. and
Strouboulis, J. Τhe Dominant Control Region of the human beta-globin domain. Ann N Y Acad
Sci., Sixth Cooley's Anemia Symposium Ed. Arthur Bank, 1990, 612, pp. 152-159.
2. Greaves, D.R., Talbot, D., Philipsen, S., Pruzina, S., de Boer, E., Hanscombe, O., Fraser, P.,
Antoniou, M., Lindenbaum, M., Dillon, N., Belhumeur, P., Strouboulis, J. and Grosveld, F.
Studies of the beta-globin gene: creation of models for somatic gene therapy. In: Application of
Molecular Genetics to the Diagnosis of Inherited Disease. Royal College of Physicians of
London, 1991, Ed. K.E. Davies, pp. 77-84.
3. Dillon, N., Fraser, P., Hanscombe, O., Greaves, D.R., Lindenbaum, M., Whyatt, D.,
Strouboulis, J., Hurst, J. and Grosveld, F. Regulation of the human gamma-globin to betaglobin switch in transgenic mice.In: The regulation of Hemoglobin Switching. Proceedings of
7th Hemoglobin Conference. Eds, G. Stamatoyannopoulos and A.W. Nienhuis. Johns Hopkins
University Press, Maryland, 1991, pp. 34-44.
4. Grosveld, F., Antoniou, M., Berry, M., de Boer, E., Dillon, N., Ellis, J., Fraser, P., Greaves, D.,
Hanscombe, O., Hurst, J., Lindenbaum, M., Mignotte, V., Philipsen, S., Pruzina, S.,
Strouboulis, J., Talbot, D. and Whyatt, D. The human beta-globin Locus Control Region.
Stohlman Lecture In: Haematology and Blood Transfusion Vol. 35, Modern Trends in Human
Leukemia IX R. Neth et al (Eds.), Springer-Verlag Berlin Heidelberg, 1992., pp. CV-CXVIII.
5. Grosveld, F., Antoniou, M., Berry, M., de Boer, E., Dillon, N., Ellis, J., Fraser, P., Greaves, D.,
Hanscombe, O., Hurst, J., Lindenbaum, M., Mignotte, V., Philipsen, S., Pruzina, S.,
Strouboulis, J., Talbot, D. and Whyatt, D. The regulation of the human beta-globin locus. In:
Applications of Basic Science to Hematopoiesis and Treatment of Disease. Ed. Donall
Thomas, Raven Press, Ltd., New York, 1991, pp. 21-38.
6. Grosveld, F., Antoniou, M., Berry, M., de Boer, E., Dillon, N., Ellis, J., Fraser, P., Hanscombe,
O., Hurst, J., Imam, A., Lindenbaum, M., Philipsen, S., Pruzina, S., Strouboulis, J., RaguzBolognesi, S. and Talbot, D. The Regulation of Human Globin Gene Switching. Phil. Trans. R.
Soc. Lond. B., 1993, 339, pp. 183-191.
7. Dillon, N., Antoniou, M., Berry, M., de Boer, E., Drabek, D., Ellis, J., Fraser, P., Hanscombe,
O., Imam, A., Koken, M-A., Lindenbaum, M., Meijer, D., Philipsen, S., Pruzina, S., RaguzBolognesi, S., Strouboulis, J., Talbot, D., Whyatt, D. and Grosveld, F. Regulation of the human
beta-globin expression domain. In: The Chromosome. Eds. J.S. Heslop-Harrison and R.B.
Flavell, Bios Scientific Publishers, 1993, pp. 149-159.
8. Grosveld, F., Antoniou, M., Berry, M., de Boer, E., Dillon, N., Ellis, J., Fraser, P., Hurst, J.,
Imam, A., eijer, D., Philipsen, S., Pruzina, S., Strouboulis, J. and Whyatt, D. Regulation of
human globin gene switching. Cold Spring Harbor Symposia on Quantitative Biology, 1993,
Vol. LVIII.
9. Dillon, N., Strouboulis, J. and Grosveld, F. The regulation of human beta-globin gene
expression: polarity of transcriptional competition in the.human beta-globin locus. Proceedings
of the Ninth Conference on Hemoglobin Switching. Ed. G. Stamatoyannopoulos, Intercept Ltd.,
1995, pp.23-28.
10. Ellis, J., Philipsen, S., Strouboulis, J., Pruzina, S., Tewari, R., Gillemans, N., Drabek, D.,
Harper, A., Grosveld, F. and Fraser, P. The regulation of human beta-globin gene expression:
the roles of 5'HS1-4. Proceedings of the Ninth Conference on Hemoglobin Switching. Ed. G.
Stamatoyannopoulos, Intercept Ltd., 1995, pp.29-38.
11. Grosveld, F., Fraser, P., Philipsen, S., Ellis, J., Wijgerde., M., Antoniou, M., Pruzina, S., Meijer,
D., Zafarana, G., Strouboulis, J. and Dillon, N. Transcriptional regulation of the beta-globin
gene cluster. In: Sickle Cell Disease and Thalassaemias: new trends in therapy. Eds. Y.
Beuzard, B. Lubin, J. Rosa. Colloque INSERM/John Libbey Eurotext Ltd., 1995, 234, pp. 1925.
12. Grosveld, F., Dillon, N., Fraser, P., Strouboulis, J. and Wijgerde, M. The regulation of human
beta-globin gene expression: the dynamics of transcriptional competition in the human betaglobin locus. NATO Advanced Study Institute on Regulation of Cell Growth, Differentiation and
Genetics in Cancer, 1997.
7
John Strouboulis
13. Published Conference Abstracts
More than twenty meeting abstracts published in the journals Blood, FEBS Journal, Blood Cells
Molecules and Diseases.
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