EUROIMMUN
Medizinische
Labordiagnostika
AG
Anti-Bartonella henselae IIFT (IgG/IgM)
Positive reaction (IgG)
Negative reaction (IgG)
Positive reaction (IgM)
Negative reaction (IgM)
Antibodies against Bartonella henselae
Indications: Test sytem for the in vitro determination of antibodies against Bartonella henselae in human serum or plasma for the diagnosis of the following diseases: cat scratch disease
(CSD), local lymphadentitis.
Clinical significance: Bartonella henselae is a gram-negative, aerobe, facultative intracellular
rod-shaped bacteria which has only been known since 1990. Today, 2 serotypes have been
isolated, Bartonella henselae type I and Bartonella henselae type II. Infections with Bartonella
henselae type II are predominant, whereas co-infections occur very rarely.
As the causative agent of the worldwide spread cat-scratch disease (CDS, synonyms: cat
scratch fever; cat scratch lymphadenitis; benign inoculation lymphoreticulosis; maladie des
griffes de chat) Bartonella henselae is of great importance. Cats only transmit the disease and
do not become infected with Bartonella henselae. The agent can be found in 10% to 70% of
cats in Germany and is presumably carried from cat to cat by cat fleas.
The generally self-limiting infection is mainly characterised by local lymphadenitis. Transmission occurs through scratches or bite wounds from mostly young cats. Following inoculation,
half of patients (mostly children) develop papulopustular primary lesions within a week, which
heal quickly. After an additional week, there is a painful lymph node swelling. This can reach
a considerable size and can develop into a chronic abscessed granulomatous inflammation.
Patients also suffer from general symptoms such as fever, headache, loss of appetite and
vomiting.
In cases of reduced immunity, Bartonella henselae causes disseminated forms of cat-scratch
disease, which are characterised by painful, vessel-rich lesions on the skin (bacillary angiomatosis), manifestations in the liver (peliosis hepatis) or in any other visceral organ and in the
central or peripheral nervous system.
Application of the Anti-Bartonella henselae IIFT: Cat scratch disease is not only found in paediatrics. It must also be taken into account in differential diagnosis of lymphadenitis in adults.
In HIV patients, every fever of unknown origin should be investigated for a Bartonella infection. Bartonella henselae infections are too rarely identified or frequently only diagnosed late
in the clinical course. Therefore, antibodies against Bartonella henselae should be investigated
using IIFT, ELISA or Westernblot in all process stages. IIFT has so far yielded the most reliable
results. IgG and/or IgM antibodies are mostly detectable at the time of lymph node swelling.
Low titers, however, are also found in patients without clinical symptoms. The prevalence
(IgG) in healthy blood donors is between 9% and 28% (patients witch CSD: 81%). Sudden titer
increases within a few weeks are particularly significant in the diagnosis.
Fluorescence pattern (positive reaction IgG): Antibodies against Bartonella henselae result in
a coarse granular fluorescence in the cytoplasm. The specific fluorescence appears in single
to numerous cells depending on the level of infection.
Fluorescence pattern (positive reaction IgM): Antibodies against Bartonella henselae cause a
coarse granular fluorescence of the cytoplasm; the granula are in part perinuclear and form
clusters. Individual coarse granula should be evaluated as unspecific.
EUROIMMUN IIFT
Infectious Serology
Viruses:
Adenoviruses
Chikungunya virus
Coxsackieviruses
Crimean Congo fever virus*
Cytomegalovirus (CMV)
Dengue viruses types 1-4
ECHO virus
Epstein-Barr virus capsid antigen (EBV-CA)
Epstein-Barr virus early antigen (EBV.EA)
Epstein-Barr virus nuclear antigen (EBNA)
Hantaviruses (types Hantaan, Puumala, Seou
Saaremaa, Dobrava, Sin Nombre, Andes)*
Herpes simplex virus types 1 and 2 (HSV-1/2)
Human herpes virus type 6 (HHV-6)
HIV-1 and -2*
Influenza virus types A and B
Japanese encephalitis virus (JEV)*
Measles virus
Mumps virus
Parainfluenza viruses types 1-4
Plasmodium falciparum HRP-2/MRP-2 (rec.)*
Plasmodium vivax MSP/CSP (recombinant)*
Respiratory syncytial virus (RSV)
Rubella virus*
Sandfly fever virus
(types Sicilian, Naples, Toscana, Cyprus)
SARS Coronavirus (SARS-CoV)
Tick-borne encephalitis (TBE) virus
Varicella zoster virus (VZV)
West Nile virus (WNV)
Yellow fever virus (YFV)
Bacteria:
Afipia felis*
Bartonella henselae
Bartonella quintana
Bordetella parapertussis
Bordetella pertussis
Borrelia afzelii
Borrelia burgdorferi
Borrelia garinii
Campylobacter coli*
Campylobacter jejuni*
Chlamydia pneumoniae
Chlamydia psittaci
Chlamydia trachomatis
Haemophilus influenzae*
Helicobacter pylori
Klebsiella pneumoniae*
Legionella bozemanii*
Legionella dumoffii*
Legionella gormanii*
Legionella jordanis*
Legionella micdadei*
Legionella pneumophila serotypes 1-14
Listeria monocytogenes 1/2 a, 4b*
Mycoplasma hominis
Mycoplasma pneumoniae
Treponema pallidum
Treponema phagedenis
Ureaplasma urealyticum
Yersinia enterocolitica*
Bacteria antigens (EUROPLUS):
Borrelia VlsE (recombinant)
Borrelia OspC
Yeasts:
Candida albicans
Candida glabrata*
Candida krusei*
Candida parapsilosis*
Candida tropicalis*
Parasites:
Echinococcus granulosus
Leishmania donovani
Plasmodium falciparum
Plasmodium vivax
Toxoplasma gondii
Profiles:
Accompanying hepatitis profile
Central nervous system profile
Exanthema profile
Fever profile South East Asia
Flavivirus profile
Gastrointestinal tract profile
Infectarthritis profile
Infectarthritis profile (The Tropics)
Lymphadenitis profile
Myocarditis profile
Ophthalmology profile
Otitis profile
Pregnancy profile
Respiratory tract profile
Sexually transmitted diseases (STD) profile
TORCH profile
* Currently not available as IVD in the EU.
Special substrate combinations
on request
Made in Germany
EUROIMMUN AG · D-23560 Luebeck (Germany) · Seekamp 31 · Phone +49 451 58550 · Fax 5855591 · E-mail [email protected] · www.euroimmun.de
EUROIMMUN
Medizinische
Labordiagnostika
AG
Test Characteristics
Anti-Bartonella henselae IIFT
EUROIMMUN IIFT
Autoimmune Diagnostics
tissue/cell substrates:
adrenal gland, monkey
bladder, rat
cartilage (trachea), monkey*
cerebellum, monkey
cerebrum, monkey
Crithidia luciliae
erythrocytes, human*
eye, monkey*
granulocytes, human (ethanol-fixed)
granolocytes, human (formaldehyde-fixed)
granulocytes, human (methanol-fixed)
heart, monkey
HEp-2 cells
HEp-20-10 cells
HUVEC
hypothalamus, monkey*
intestine, monkey
kidney, monkey
kidney, mouse
kidney, rat
lacrimal gland, monkey
lip, monkey*
liver, monkey
liver, mouse
liver, rat
lobus temporalis, monkey*
lung, monkey
lymph nodes, monkey*
lymphocytes, human*
mamma, monkey*
mouth mucosa, monkey*
nerve, monkey
oesophagus, monkey
oesophagus, rat
ovary, monkey
pancreas, monkey
parathyroid gland, monkey
parotid gland, monkey
pituitary gland, monkey
placenta, monkey*
prostate, monkey
Saccharomyces cerevisiae
skeletal muscle, monkey
spermatozoa, human
spleen, monkey*
spinal cord, monkey
stomach, monkey
stomach, mouse
stomach, rat
synovium, monkey
testis, monkey
thrombocytes, human
thymus, monkey
thyroid gland, monkey
tongue, monkey
VSM47 cells
umbilical cord, human
EUROPLUS™ substrates:
AIH (LC-1 + SLA/LP)
AMA-M2
gliadin
intrinsic factor
myeloperoxidase (MPO)
PBC (AMA M2 + Sp100)
proteinase 3 (PR3)
ribosomal P-proteins + Jo-1
SS-A + SS-B
SS-B + ribosomal P-proteins + Jo-1
SS-B + Scl-70 + Jo-1
thyroglobulin (TG)
Test principle: The indirect immunofluorescence test is a
standardized in vitro assay for the determination of specific
antibodies against Bartonella henselae. BIOCHIPs are coated with Bartonella henselae infected cells and fixed onto
the reaction fields of a microscope slide. With EUROIMMUN
BIOCHIP Mosaics™, different substrates can be positioned
next to each other in one reaction field and incubated with
one serum sample, allowing a detailed patient antibody
profile to be established with a single test.
Test procedure: EUROIMMUN BIOCHIP slides are incubated
using the proprietary TITERPLANE™ Technique, which enables multiple samples to be incubated next to each other
and simultaneously under identical conditions. Results are
evaluated by fluorescence microscopy. Incubation of the
substrate with the positive and negative control sera provided in each kit verifies correct performance of the test and
aids evaluation.
Incubation with the
TITERPLANE™ Technique
Reagent
tray
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Slide
fitted with
BIOCHIPs
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Inter-lot reproducibility: Inter-lot reproducibility was tested with more than 10 different lots. In
quantitative evaluation of results, the deviation amounted to no more than ± 1 fluorescence
intensity level.
Reference range: Sera from healthy blood donors were investigated for anti-Bartonella henselae antibodies using the EUROIMMUN IIFT. For IgG, 26.5% of the blood donors (n = 200) were
positive with a cut-off titer of 1:320 or higher. For IgM, 1.0% of the blood donors (n = 200) were
positive with a cut-off titer of 1:100 or higher.
Specificity: The specificity of this test system for antibodies of the class IgG is 84%. Reference: Anti-Bartonella henselae IIFT (n = 215), origin of samples: Germany. The specificity for
antibodies of the class IgM is 88%. Reference: Anti-Bartonella henselae IIFT (n = 37), origin of
samples: Europe.
Sensitivity: The sensitivity of this test system for antibodies of the class IgG is 80%. Reference: Anti-Bartonella-henselae-IIFT (n = 215), origin of samples: Germany. The sensitivity for
antibodies of the class IgM is 100%. Reference: Anti-Bartonella-henselae-IIFT (n = 37), origin of
samples: Europe.
Technical Data:
Antigen substrate
Bartonella henselae infected cells (species EU 70).
Sample dilution
Serum or plasma.
Cut-off: 1:320 (for IgG), 1:100 (for IgM)
Qualitative evaluation: 1:100 (for IgG and IgM)
Quantitative evaluation: 1:100/1000/10000, etc. (for IgG and IgM).
Test procedure
IgG: 30 min (sample) / 30 min (conjugate), room temperature.
IgM: 60 min (sample) / 30 min (conjugate), 37°C
Microscopy
Objective 40x
Excitation filter: 488 nm, colour separator: 510 nm, blocking filter:
520 nm
Light source: EUROIMMUN LED or mercury vapour lamp, 100 W
Reagents
Ready for use, with the exception of the PBS-Tween buffer (for dilution and washing).
Stability
All kit components are stable for up to 18 months from the date of
manufacture.
Standard kit formats
10 or 20 slides, each containing 3, 5 or 10 test fields.
Kits include all necessary reagents (except EUROSORB for the RFabsorption, order no.: ZF1270-0145).
Made in Germany
Order no.
FI 219b-1005 G or M
Version: 01/07
FI_219b_D_UK_A02
Related products
FI 219b-1005-1 G or M (IIFT Mosaic: B. henselae and B. quintana)
BIOCHIP MosaicsTM:
ANA global test: HEp-20-10/monkey liver
Autoantibody Profile: combination of
30 different tissues per slide
CIBD Profile: monkey pancreas/intest. goblet cells (culture)/
granulocytes (EOH)/Saccharomyces cerevisiae
Basic Profile: HEp-20-10/monkey liver/
rat kidney/rat stomach
EUROPLUS endomysium + gladin:
monkey intestine/monkey liver/gliadin
Granulocyte Mosaic: granulocytes (EOH)/
granulocytes (HCHO)/HEp-2/monkey liver
Liver Mosaic: HEp-2/monkey liver/rat liver
rat kidney/rat stomach/monkey heart
Neuronal Antibody Screen: monkey cerebellum/
monkey nerve/monkey intestine
Polyendocrinopathy Mosaic: monkey thyroid/
monkey pancreas/monkey adrenal/
monkey ovary/monkey testis/monkey stomach
Other mosaics also available
Special substrate combinations
on request
* Currently not available as IVD in the EU.
EUROIMMUN AG · D-23560 Luebeck (Germany) · Seekamp 31 · Phone +49 451 58550 · Fax 5855591 · E-mail [email protected] · www.euroimmun.de