Uniquely aligned (A) (B) Aligned two times Perfect match 1 mismatch 2 mismatch 20 0.6 0.6 2 3 4 5 6 7 8 9 10 11 12 13 14 Log2(Reads count) 15 16 17 18 19 0 1 0 0 0 -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 Log2(RPKM) 6 7 8 9 10 11 12 13 Standard Deviation Frequency (%) 15 10 Standard Deviation 5 15 10 5 0 Frequency (%) Sib -/rx3 25 (D) Sib -/rx3 20 (C) 25 Aligned >=three times Reads without alignment
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