List of Plates Plate No 1.1 Title Page No Indian Major carp, catla, Cat/a cat/a (Hamilton- 20 Buchaman) 1.2 Metaphase spread of Indian major carp catla Cat/a 20 cat/a showing chromosome number 2n = 50. 1.3 Karyotype of Indian major carp catla, Cat/a cat/a. 20 Chromosome formula is 12M+ 16SM+ 16ST+6T. 1.4 Indian major carp, rohu, Labeo rohita (Hamilton- 22 Buchanan) 1.5 Metaphase spread of Indian major carp rohu, Labeo 22 rohita showing chromosome number 2n50. Magnification 500X 1.6 Karyotype of Indian major carp rohu Labeo rohita. 22 Chromosome formula is 10M+18SM+10ST+12T. 1.7 Indian major carp, mrigal, Cirrhthus mngaia 25 (Hamilton- Buchanan) 1.8 Metaphase spread of Indian major carp mrigal, 25 Cirrhin us mngala showing chromosome number 2n50. 1.9 Karyotype of Indian major carp mrigal, Girrhinus 25 mngala Chromosome formula is 12M + 18SM+ lOST + 12T. 1.10 Silver carp, Hypohthalmichthys molitrix (Valenciennes) 28 Plate No 1.11 Title Page No Metaphase spread of silver carp, Hypohthalmichthys 28 molitnx, showing chromosome number 2n=48. 1.12 Karyotype of silver carp, Hypohthahnichthys 28 molitnx, Chromosome formula is 22M+14SM+12T. 1.13 Common carp, Cyprinus carpio. (Linnaeus) 1.14 Metaphase spread of common carp, 32 Cyprinus carpio 32 showing chromosome number 2n=100. 1.15 Karyotype of common carp, Cyprinus carpio. 32 Chromosome formula is 24M+24SM+16ST+36T. 1.16 Goldfish 1.17 Carassius auratus (Linnaeus) Metaphase spread of gold fish 36 Carassius auratus 36 showing chromosome number 2n=100. 1.18 Karyotype of gold fish Cars ssi us auratus. 36 Chromosome formula is 18M+82A 1.19 Stream fish, 1.20 Pun tius ticto (Hamilton-Buchanan) 41 Puntius ticto 41 Pun tius ticto. Chromosome 41 Metaphase spread of stream fish, showing chromosome number 2n50. 1.21 Karyotype of stream fish, formula is 6M+14SM+8ST+22T 1.22 1.23 Stream fish, Punt] us amphibius. (Valenciennes) Metaphase spread of stream fish, Pun tius amphibius 44 44 showing chromosome number 2n=44. 1.24 Karyotype of stream fish, Pun tius amphibius. Chromosome formula is 4M+2SM+38T 44 Plate No 1.25 1.26 Title Page No Stream fish, Puntju5 dorsths. (Jerdon) Metaphase spread of stream fish, 47 Puntius dorsjth5 47 showing chromosome number 2n=50. 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 Karyotype of stream fish, Puntius dorsths. Chromosome formula is 4M+12ST+34T 47 Stream fish, Puntiusjerdom (Day) 51 Metaphase spread of stream fish, Puntius jerdoni showing chromosome number 2n=50. 51 Karyotype of stream fish, Puntius jerdoni. Chromosome formula is 4M+ 1 2ST+34T 51 Stream fish, Pun this vittatus. (Day) 54 Metaphase spread of stream fish, Puntiu5 vittatus showing chromosome number 2n=50. 54 Karyotype of stream fish, Puntius vittatus. Chromosome formula is 6M+12SM+10ST+22T 54 Stream fish, Puntius arulius tampiraparue i - Silas Fish collected from upper Tampiraparanj 57 Metaphase spread of stream fish, Puntius arulius tampirapnjej showing chromosome number 2n=48. 57 Karyotype of stream fish, Puntius arulius 57 tampiraparmel Chromosome formula is 20M + 16ST + 12T 1.37 Stream fish, Punthis arulius tampiraparnj Silas. Fish collected from Kanadjan canal and lower Tampiraparani river system 58 Plate No 1.38 Title Page No Metaphase spread of stream fish, Pun tius arulius 58 tampirapariiiei showing chromosome number 2n50. 1.39 Karyotype of stream fish, Pun tius arulius 58 tampiraparniei. Chromosome formula is 22M + 14ST +12T 1.40 Stream fish, 1.41 Rasbora daiiiconius. (Jerdon) 62 Rasbora daniconius 62 Metaphase spread of stream fish, showing chromosome number 2n=48. 1.42 Karyotype of stream fish, Rasbora daniconius. 62 Chromosome formula is 18M+ 18SM+ 12T 1.43 Stream fish, Puntius filainentosus (Valenciennes) 65 collected from Kanadian canal Pabanasam river and lower Tampiraparani river system 1.44 Metaphase spread of stream fish, Puntius 65 filamentosus showing chromosome number 2n=48. 1.45 Karyotype of stream fish, Puntius filamentosus 65 Chromosome formula is 22M+26ST 1.46 Stream fish, Pun tius ifiamentosus (Valenciennes) W. collected from upper Tampiraparani river system 1.47 Metaphase spread of stream fish, Pun tius filamentosus showing chromosome number 2n50. 1.48 Karyotype of stream fish, Puntius filamentosus Chromosome formula is 20M+30ST 1.49 Stream fish, Danio aeqwpinnatus. (Mc Clelland) 70 Plate No 1.50 Title Page No Metaphase spread of stream fish, Damo 70 aequipinnatus showing chromosome number 2n=50. 1.51 Karyotype of stream fish, Darno aeqwjinnatus. 70 Chromosome formula is 6M+6SM+32ST+6T Gara mullya. (Sykes) 1.52 Stream fish, 1.53 Metaphase spread of stream fish, 73 Gara mullya 73 Gara mullya. Chromosome 73 showing chromosome number 2n50. 1.54 Karyotype of stream fish, formula is 6M+18SM+125T+14T 1.55 Stream fish, Amblypharyngodan microlepis. (Bleeker) 76 1.56 Metaphase spread of stream fish, Amblypharyngodan 76 microlepis showing chromosome number 2n52. 1.57 Karyotype of stream fish, Amblypharyngodan 76 microlepis. Chromosome formula is 10M+18SM+24T Salinostoma clupeoidus. (Bloch) 1.58 Stream fish, 1.59 Metaphase spread of stream fish, Salmostoma 80 80 clupeoidus showing chromosome number 2n=48. 1.60 Karyotype of stream fish, Salinostoma clupeoidus. 80 Chromosome formula is 4M+44T Mystus armatus (Day) 1.61 Cat fish, 1.62 Metaphase spread of cat fish, 83 Mystus arm atus. 83 showing chromosome number 2n=54. 1.63 Karyotype of cat fish, Mystus armatus. Chromosome formula is 10M+24SM+4ST+16T. 83 Plate No 1.64 1.65 Title Cat fish, Mystus Page No keletius (Valenciennes) Metaphase spread of cat fish, 87 Mystus keletius 87 showing chromosome number 2n=54. 1.66 Karyotype of cat fish, Mystus keletius. Chromosome 87 formula is 6M+20SM+28T 1.67 Catfish, 1.68 Metaphase spread of cat fish, Ompok bthiacujatus (Bloch) all Ompok bimacufatus showing chromosome number 2n=40. 1.69 Karyotype of cat fish, Ompok biinaculatus. Chromosome formula is 1OM+8SM+22T Heteropneustes fossiJi5. (Bloch) 170 Cat fish, 1.71 Metaphase spread of cat fish, 93 Heteropneustesfossths 93 showing chromosome number 2n=45. 1.72 Karyotype of cat fish, Heteropneustes fossilis. 93 Chromosome formula is 14M+24SM+8sT+12 1.73 Cichild, Oreochromjs mossainbicus. (Peters) 1.74 Metaphase spread of cichlid, Oreochromis 97 97 mossambicus showing chromosome number 2n44. 1.75 Karyotype of cichlid, Oreochromis mossambicus. 97 Chromosome formula is 4ST+40T Etroplus maculatus. (Bloch) 1.76 Cichlid, 1.77 Metaphase spread of cichlid, Etroplus macu/titus showing chromosome number 2n=48. 100 100 Title Page No Karyotype of cichlid, Etroplus maculatus. 100 Plate No 1.78 Chromosome formula is 48A 1.79 Stream fish, Macropodus cupanous (Valenciennes) 104 1.80 Metaphase spread of stream fish, Macropodus 104 cupanous showing chromosome number 2n-48. 1.81 Karyotype of stream fish, Macropodus cupanous. 104 Chromosome formula is 32M+ 14SM+2T 1.82 Murrel, Channa stnatus. (Bloch) 107 1.83 Metaphase spread of murrel, Channa striatus 107 showing chromosome number 2n=42. 1.84 Karyotype of murrel, Channa stnatus. Chromosome 107 formula is 14M+125M+14ST 1.85 Murrel, Channapunctatus. (Bloch) 111 1.86 Metaphase spread of murrel, Channa punctatus 111 showing chromosome number 2n=32. 1.87 Karyotype of murrel, Channa pun ctatus. 111 Chromosome formula is 14M+12SM+6ST 2.1 G-banded metaphase spread of Indian major carp, 148 rohu, Labeo rohita showing chromosome number 2n50. 2.2 G-banded metaphase spread of Indian major carp, 148 rohu, Labeo rohita showing chromosome number 2n50. 2.3 G-banded metaphase spread of common carp, (]ypiinus carpio showing chromosome number 2n100. 149 Plate No 2.4 Title Page No G-banded karyotype of common carp, Cyprinus 149 carplo. showing chromosome number 2n=100. 2.5 G-banded metaphase spread of stream fish, Puntius 150 dorsalis showing chromosome number 2n=50. 2.6 G-banded karyotype of stream fish, Pun tius dorsalis 150 showing chromosome number 2n50. 2.7 G-banded metaphase spread of cat fish, Mystus 152 arm atus showing chromosome number 2n54. G-banded karyotype of cat fish, Mystus arm atus 152 showing chromosome number 2n=54. 2.9 G-banded metaphase spread of cichiid, Etroplus 153 maculatus showing chromosome number 2n48. G-banded karyotype of cichlid, Etroplus maculates 2.10 153 showing chromosome number 2n48. 2.11 G-banded metaphase spread of murrel, Channa 154 striatus showing chromosome number 2n=42. 2.12 G-banded karyotype of murrel, Channa stratus 154 showing chromosome number 2n=54 2.13 G-banded metaphase spread of murrel, Channa 155 pun ctatus showing chromosome number 2n32. 2.14 G-banded karyotype of murrel, Channa punctatus 155 showing chromosome number 2n=32. 2.15 Metaphase spread of rohu Labeo rohita showing NOR bands. 158 Plate No Title Page No Labeo rohita. 2.16 NOR banded karyotype of rohu, 2.17 Metaphase spread of common carp 158 Cypthius carpio 159 jerdoni 160 NOR banded karyotype of stream fish, Puntius 160 showing NOR bands. 2.18 Metaphase spread of stream fish, Puntius showing NOR bands. 2.19 jerdonl. 2.20 Salinostoma 162 banded karyotype of stream fish, 162 Metaphase spread of stream fish, ciupeoidus showing NOR bands. 2.21 NOR Sali-nostoma clupeoidus. 2.22 dorsails 163 NOR banded karyotype of stream fish, Puntius 163 Metaphase spread of stream fish, Puntlus showing NOR bands. 2.23 dorsails. 2.24 Metaphase spread of cat fish, Heteropnestus fossths 164 showing NOR bands. Heteropnestus fossiiis. 2.25 NOR banded karyotype of 2.26 Metaphase spread of cichlid, 164 Etroplus maculatus 165 Etropius maculatus 165 Showing the water bath with thermostat control and 188 showing NOR bands. 2.27 NOR banded karyotype of cichlid, showing NOR bands. 3.1 the way by which thermal shock was given. Plate No Title Page No 3.2 Normal 4 celled stage of diploid rohu, Labeo rohita. 196 3.3 Highly proliferated cells at 4 celled stage of triploid 196 rohu, Labeo rohita. 3.4 16 celled stage of diploid rohu, Labeo rohita. 197 3.5 Continuos involution of excess cells synthesized in 16 197 celled stage of triploid rohu, Labeo rohita. 3.6 a and b comparison of diploid and triploid larval 198 stages of rohu, Labeo rohita. c and d comparison of diploid and triploid larval stages of rohu, Labeo rohita. 3.7-3.8 Comparision of embryonic developmental stages of 199 diploid and triploid catla, Cat/a cat/a. a - Diploid b - Tnploid Stages of the embryonic development of diploid Labeo MO rohita 3.10 3.11 (after spawning). Stages of the embryonic development of rohu, Labeo rohita 200 201 after ploidy induction. Stages of the embryonic development of diploid, catla 202 Cat/a cat/a (after spawning). 3.12 Stages of the embryonic development of catla, Cat/a 203 cat/a after ploidy induction. 3.13 Metaphase spread of diploid rohu, Labeo rohita showing diploid chromosome number 2n50. 206 Plate No 3.14 3.15 Title Karyotype of diploid rohu, Page No Labeo rohita 2n50 Metaphase spread of triploid rohu, Labeo rohita 206 207 showing diploid chromosome number 2n50. 3.16 Karyotype of triploid rohu, 3.17 Metaphase spread of diploid catla, Labeo rohita 3n75. 207 Cat/a cat/a 208 Cat/a cat/a showing 208 Cat/a cat/a 209 showing chromosome number 2n=50. 3.18 Karyotype of diploid catla, chromosome number 2n=50. 3.19 Metaphase spread of triploid catla, showing chromosome number 3n75. 3.20 Karyotype of triploid catla, Catla cat/a showing 209 Growth comparison of triploid (below) and diploid 211 chromosome number 3n=75. 3.21 (above) of rohu, Labeo rohita. 3.22 Comparison of growth of triploid (above) and diploid (below) catla 3.23 3.24 3.25 3.26 3.27 Cat/a cat/a. Labeo rohita. 221 Erythrocytes from triploid (3n) rohu, Labeo rohita. 221 Erythrocytes from diploid (2n) rohu, 211 Erythrocytes of diploid (2n) catla, Cat/a cat/a. 222 Erythrocytes of triploid (3n) catla, Cat/a cat/a. 222 Highly proliferation of white bubbles mass is due to abnormal embryonic development of triploid rohu, Labeo rohita. 225 Title Page No Morphological differences of color, scale (paled 226 Plate No 3.28 3.29 nature) and fin size (damaged) during growth performance of triploid catla, Cat/a cat/a. Abnormal embryos from fertilized eggs of rohu as well as catla 227
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