Poster Note 71 985
A New Anion Exchange Column for
Fast Ion Chromatographic Separation of
A New Anion Exchange Column for Fast Ion Chromatographic Sep
Monosaccharides
andfor
Disaccharides
in
A New Anion
Exchange Column
Fast Ion Chromatographic
Sep
Beverage Samples
Biofuel,
Food,Column
and Beverage
ABeverage
New Anion
Exchange
for Fast Samples
Ion Chromatographic Sep
Samples
Yan Liu, Andy Woodruff, Charanjit Saini, Yury Agroskin, Chris Pohl
Beverage
Samples
Yan Liu, Andy
Woodruff,
Charanjit
Yury
Yan
Liu,
Andy Woodruff, Charanjit
Saini, YurySaini,
Agroskin, Chris
Pohl Agroskin, Chris Pohl
Thermo Fisher
Scientific,
1228
Titan
Way,
Sunnyvale, CA 94086
Thermo
Fisher Scientific, 1228
Titan Way,
Sunnyvale,
CA 94086
Yan
Liu, Andy
Saini,
Yury
Agroskin,
Chris
Pohl
Thermo
FisherWoodruff,
Scientific,Charanjit
1228 Titan
Way,
Sunnyvale,
CA
94086
Thermo Fisher Scientific, 1228 Titan Way, Sunnyvale, CA 94086
Overview
Overview
Purpose:
New Thermo Scientific
TM DionexTM CarboPacTM SA10-4µm anion exchange
columns designed specifically to provide
exceptionally fast, high-resolution separation
TM DionexTM CarboPacTM SA10-4µm anion exchange
Purpose:
New
Thermo
Scientific
of monosaccharides and disaccharides
are developed.
columns designed specifically to provide exceptionally fast, high-resolution separation
Methods:
The CarboPac
columns
are packed
with a hydrophobic,
of monosaccharides
and SA10-4μm
disaccharides
areTMdeveloped.
TM
TM
Purpose:
Thermo Scientific
anionofexchange
polymeric,New
supermacroporous
anionDionex
exchangeCarboPac
resin stableSA10-4µm
over the range
pH 0-14.
columns
designed
specifically
to
provide
exceptionally
fast,
high-resolution
separation
Methods:
The
CarboPac
SA10-4μm
columns
are
packed
with
a hydrophobic,
In comparison to the CarboPac SA10 column, the CarboPac
SA10-4μm
column
of
monosaccharides
and
disaccharides
are
developed.
polymeric,
supermacroporous
anion
exchange
resin
stable
over
the
range
of
pH the
0-14.
exhibits higher peak efficiency while maintaining the same selectivity. This is due
In comparison
to the
CarboPac
SA10
column,
thetoCarboPac
SA10-4μm
column
fact
that the
same
functional
groups
are
attached
the smaller
particles
used in
Methods:
The
CarboPac
SA10-4μm
columns
are packed
with a resin
hydrophobic,
exhibits
higherSA10-4μm
peak efficiency
while maintaining
same
selectivity.
is in
due the
the
CarboPac
column.
reducing resin
thethe
resin
particle
sizerange
toThis
4 μm
polymeric,
supermacroporous
anionByexchange
stable
over the
of pH
0-14.
fact that the
same functional
groupsby
are
to the smaller
resin particles
used in
diameter,
efficiencies
are increased
upattached
to 50%
without
anySA10-4μm
compromise
in selectivity
In
comparison
to the CarboPac
SA10 column,
the CarboPac
column
the
CarboPac
SA10-4μm
column.
By
reducing
the
resin
particle
size
to
4
μm
in
or capacity.
exhibits
higher peak efficiency while maintaining the same selectivity. This is due the
diameter, efficiencies are increased by up to 50% without any compromise in selectivity
fact
that the
same
functional
groups are separations
attached to the
smaller resin particles
in
Results:
The
rapid
and high-resolution
of carbohydrates
such as used
fucose,
or capacity.
the
CarboPac
SA10-4μm
column.
By reducing
the
resin particle
size to 4inμm
in
sucrose,
arabinose,
galactose,
glucose,
xylose,
mannose
and fructose
biofuel
diameter,
efficiencies
are increased
by upas
tosucrose,
50% without
any compromise
inas
selectivity
Results:as
The
and
high-resolution
separations
of carbohydrates
fucose,
samples
wellrapid
as carbohydrates
such
glucose,
fructose, such
lactose,
or
capacity.arabinose,
sucrose,
galactose,
glucose,
xylose,
mannose
and fructose in biofuel
cellibiose,
and maltose
in food and
beverage
samples
are obtained.
samples as well as carbohydrates such as sucrose, glucose, fructose, lactose,
Results: The rapid and high-resolution separations of carbohydrates such as fucose,
cellibiose, and maltose in food and beverage samples are obtained.
sucrose, arabinose, galactose, glucose, xylose, mannose and fructose in biofuel
samples as well as carbohydrates such as sucrose, glucose, fructose, lactose,
cellibiose, and maltose in food and beverage samples are obtained.
Overview
Introduction
Introduction
The
biofuel, food, and beverage industries process very high volumes of carbohydrate-
containing samples. These industries require high throughput determination of
The biofuel, food, and
and disaccharides
beverage industries
process
verymatrices.
high volumes
of carbohydratemonosaccharides
in various
sample
We have
recently
containingnew
samples.
These
industries
require
throughput
determination
of
TM Dionex
TM high
TM SA10-4µm
CarboPac
anion exchange
developed
Thermo
Scientific
monosaccharides
and
disaccharides
in
various
sample
matrices.
We
have
recently
columns
designed
specifically
provide
exceptionally
high-resolution
separation
The
biofuel,
food, and
beveragetoindustries
process
veryfast,
high
volumes of carbohydrateTM
TM
TM
Dionex
CarboPac SA10-4µm
exchange
developed
new Thermo
of
monosaccharides
and Scientific
disaccharides.
Thehigh
characteristics
the anionanion
exchange
containing
samples. These
industries require
throughput of
determination
of
columns designed
specifically
to provide
exceptionally
fast,
high-resolution
separation
SA10-4µm
columns
are described.
The
stationary
phase used
in the new
CarboPac
monosaccharides
and disaccharides
in various
sample matrices.
We
have recently
of monosaccharides
andcolumns
disaccharides.
The
characteristics
of the anion exchange
TM CarboPac
performance
the new
in the
determination
ofTM
monosaccharides
Dionex
SA10-4µm anionand
exchange
developed
newofThermo
ScientificTM
SA10-4µmsystems
columnswith
are electrolytic
described. eluent
The
stationary phase
used
in pressure
the new CarboPac
disaccharides
using
high
ion
chromatography
columns designed specifically to provide exceptionally fast, high-resolution separation
performance
the new columns
in the determination
monosaccharides
and
generation
andofelectrochemical
detection
capabilities
isofdiscussed.
The exchange
rapid
and
of
monosaccharides
and disaccharides.
The
characteristics
of the anion
disaccharides using
high pressure
ion chromatography
systems
with electrolytic
eluent
high-resolution
carbohydrates
such as fucose,
sucrose,
arabinose,
columns
are described.
The
stationary
phaseseparations
used in the of
new
CarboPac SA10-4µm
generationglucose,
and electrochemical
detectionfructose
capabilities
is discussed.
rapid
and
galactose,
xylose,
mannose
in biofuel
samplesThe
as well
performance
of the new
columns
in theand
determination
of monosaccharides
andas
high-resolution
separations
of
carbohydrates
such
as
fucose,
sucrose,
arabinose,
carbohydratesusing
suchhigh
as sucrose,
fructose, lactose,
cellibiose,
and maltose
in
disaccharides
pressureglucose,
ion chromatography
systems
with electrolytic
eluent
galactose,
glucose,samples
xylose, mannose
and fructose in biofuel samples as well as
food
and beverage
aredetection
demonstrated.
generation
and electrochemical
capabilities is discussed. The rapid and
carbohydrates such as sucrose, glucose, fructose, lactose, cellibiose, and maltose in
high-resolution separations of carbohydrates such as fucose, sucrose, arabinose,
food and beverage samples are demonstrated.
galactose, glucose, xylose, mannose and fructose in biofuel samples as well as
carbohydrates such as sucrose, glucose, fructose, lactose, cellibiose, and maltose in
food
are column
demonstrated.
The and
newbeverage
CarboPacsamples
SA10-4µm
is composed of a wide-pore macroporous
substrate coated with a strong anion-exchange layer of latex nano beads. The
The new resin
CarboPac
SA10-4µm
column is composed
of awith
wide-pore
macroporous
substrate
is made
of ethylvinylbenzene
crosslinked
divinylbenzene
with
substrate coated
with athe
strong
anion-exchange
layerstructure
of latex nano
beads.
The
macropores
throughout
bead.
The highly porous
results
in increased
substrate
resin
is madehigher
of ethylvinylbenzene
crosslinked
with divinylbenzene
with
surface
providing
The of
nanobead
anion-exchange
The
new area,
CarboPac
SA10-4µm loading
column capacity.
is composed
a wide-pore
macroporouslayer
macropores
throughout
the bead.
The highly porous
structure
results in increased
which
coats
the
entire
is functionalized
with an
alkanol
quaternary
ammonium
substrate
coated
with asurface
strong anion-exchange
layer
of latex
nano
beads. The
surface
area,
providing
higher
loading
capacity.
The
nanobead
anion-exchange
layer
group. This
layer
has a of
controlled
thickness which
resultswith
in excellent
mass-transfer
substrate
resin
is made
ethylvinylbenzene
crosslinked
divinylbenzene
with
which coats the
entire
surface is functionalized
with
an alkanol
quaternaryofammonium
characteristics
and
consequently,
high-efficiency
peaks.
The combination
the high
macropores
throughout
the bead. The
highly porous
structure
results in increased
group. This
layer has
a controlled
thickness
which
results
in excellent
mass-transfer
capacity
provided
by the
substrate
andcapacity.
the new
internal
chemistry
of the
nanobead
surface
area,
providing
higher
loading
The
nanobead
anion-exchange
layer
characteristics
and consequently, high-efficiency
peaks.time.
The Table
combination
of the high
functionality
delivers
and short analysis
1 summarizes
the
which
coats the
entirehigh-resolution
surface is functionalized
with an alkanol
quaternary
ammonium
capacity
provided
by
the
substrate
and
the
new
internal
chemistry
of
the
nanobead
SA10-4µm
columns
specifications
of CarboPac
group.
This layer
has a controlled
thickness
which results in excellent mass-transfer
functionality delivers high-resolution and short analysis time. Table 1 summarizes the
characteristics and consequently, high-efficiency peaks. The combination of the high
specifications of CarboPac SA10-4µm columns
capacity provided by the substrate and the new internal chemistry of the nanobead
™ Dionex™ ICS-5000+ RFIC™
All experiments
werehigh-resolution
performed using
Scientific
functionality
delivers
andThermo
short analysis
time.
Table 1 summarizes the
systems with of
electrolytic
generation.
A typical Dionex
ICS-5000+ system consists
SA10-4µm
columns
specifications
CarboPaceluent
™ Dionex™ ICS-5000+ RFIC™
All
experiments
were
performed
using
Thermo
Scientific
of a dual pump module (DP), an eluent generator (EG) module, and a +
consists
systems with electrolytic eluent
A typical
Dionexcell.
ICS-5000
detector/chromatography
modulegeneration.
(DC) with an
ED detector
Ag/AgClsystem
was used
as
of a dual pump
module (DP),
eluentelectrode.
generatorThe
(EG)™module, ™
and a
+ RFIC
™
reference
electrode
Au as an
working
used
Dionex waveform
ICS-5000was
All
experiments
wereand
performed
using Thermo
Scientificquadruple
detector/chromatography
module
(DC)
with
an
ED
detector
cell.
Ag/AgCl
was
used
as
+
+
RFICICS-5000
systems are
fully consists
for detection
of carbohydrates.
The DionexAICS-5000
system
systems
with electrolytic
eluent generation.
typical Dionex
reference by
electrode and
Au as working
electrode.
The ™
quadruple
waveformData
was used
™ Chromeleon
Dionex
Chromatography
supported
of
a dual pumpThermo
moduleScientific
(DP), an™eluent
generator
(EG) +module,
and a
for detection
Dionex ICS-5000 RFIC systems are fully
System
(CDS)of7carbohydrates.
software.module The
detector/chromatography
(DC) with an ED detector cell. Ag/AgCl was used as
supported by Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data
reference electrode and Au as working electrode. The quadruple waveform was used
System (CDS) 7 software.
for detection of carbohydrates. The Dionex ICS-5000+ RFIC systems are fully
supported by Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data
System (CDS) 7 software.
Introduction
Methods
Methods
Methods
Table 1. Specifications of DionexTM CarboPacTM SA10-4µm columns
Table 1. Specifications of DionexTM CarboPacTM SA10-4µm columns
Particle Size:
4 μm
Pore
Size:
Supermacroporous
(2000 Å) TM SA10-4µm columns
Particle
4 μm
Table
1.Size:
Specifications
of DionexTM CarboPac
Cross-linking:
55%
Pore Size:
Supermacroporous (2000 Å)
Particle
Size: capacity:4290
μm μeq per 4 × 250 mm column; 72.5 μeq per 2 × 250 mm column.
Ion
exchange
Cross-linking:
55%
Pore
Size:
(2000
Å)
174
mm
Ion exchange
capacity:Supermacroporous
290μeq
μeqper
per44××150
250
mmcolumn;
column;43.5
72.5μeq
μeqper
per22××150
250column.
mm column.
Cross-linking:
55%
Functional Group:
Quaternary
ammonium
ioncolumn; 43.5 μeq per 2 × 150 column.
174 μeq per
4 × 150 mm
Ion
exchange
capacity: 290
μeq per 4
× 250 mmion
column; 72.5 μeq per 2 × 250 mm column.
Latex
Diameter:
55
nm
Functional
Group:
Quaternary
ammonium
174
μeq
per
4
×
150
mm
column;
43.5 μeq per 2 × 150 column.
Latex
Cross-linking:
4.5%
Latex Diameter:
55 nm
Functional
Group:
Quaternary
ammonium ion
pH
range:
0-14
Latex
Cross-linking:
4.5%
Latex
Diameter:
55
nm
Temperature
Limit:
4-60°C
pH range:
0-14
Latex
Cross-linking:
Pressure
Limit:Limit: 4.5%
5000
psi
Temperature
4-60°C
pH
range:Solvent
Organic
100%
Pressure
Limit: Limit: 0-14
5000 compatible
psi
Temperature
Limit:Limit:4-60°C
Typical
Potassium
hydroxide or Sodium hydroxide
OrganicEluents:
Solvent
100% compatible
Pressure
Limit:
5000
psi
Typical Eluents:
Potassium
hydroxide or Sodium hydroxide
Organic Solvent Limit: 100% compatible
Typical Eluents:
Potassium hydroxide or Sodium hydroxide
Results
Results
The
CarboPac SA10-4μm columns are composed of a unique 4 μm
supermacroporous (SMP) substrate coated with a strong anion exchange layer of latex
The CarboPac SA10-4μm columns are composed of a unique 4 μm
nano beads, which provides high capacity and rapid, high resolution separations. The
Results
supermacroporous
substrate coated
with
a strong
exchange
layer of latex
CarboPac
SA10-4μm(SMP)
anion-exchange
column
is used
withanion
pulsed
amperometric
nano beads,
which
provides
high
capacity
and rapid,
high resolution
separations. The
detection
(PAD)
systems
and
permits
direct
quantification
nonderivatized
The
CarboPac
SA10-4μm
columns
arethe
composed
of a unique of
4 μm
CarboPac SA10-4μm
anion-exchange
column isThe
used
with pulsed
amperometric
carbohydrates
with(SMP)
minimal
sample coated
preparation.
column
high
supermacroporous
substrate
with a strong
anionprovides
exchange
layer of latex
detection (PAD)
systems
and permits
theunder
directhigh
quantification
of nonderivatized
reproducibility,
and
1000 injection
cycles
pH
possible. The
nano
beads, which
provides
high capacity
and rapid,
highconditions
resolutionare
separations.
carbohydrates with minimal sample preparation. The column provides high
CarboPac SA10-4μm anion-exchange column is used with pulsed amperometric
reproducibility, and 1000 injection cycles under high pH conditions are possible.
detection (PAD) systems and permits the direct quantification of nonderivatized
The biofuel, food,
beverage
industries
process
highprovides
volumeshigh
of carbohydrate
carbohydrates
withand
minimal
sample
preparation.
Thevery
column
samples, and require
high
throughput
mono
and
di-saccharide
analysis
capabilities.
reproducibility,
and 1000
injection
cycles
under
high
pH conditions
are possible.
TheCarboPac
biofuel, food,
and beverage
process
very high
volumes
of carbohydrate
The
SA10-4μm
columnindustries
is specifically
designed
for the
exceptionally
fast,
samples, and require
throughput mono
and di-saccharide
capabilities.
high-resolution
analysishigh
of carbohydrate
samples
for biofuel andanalysis
food applications.
The CarboPac
SA10-4μm
column
is specifically
designed
for the
exceptionally
fast,
Rapid
separation
of the
eight
most
common
biofuel
monodisaccharides
can
be
The
biofuel,
food, and
beverage
industries
process
very
highand
volumes
of carbohydrate
high-resolution
analysis
of carbohydrate
samples ×for
biofuel
food applications.
achieved
on the
(a) CarboPac
SA10-Fast-4μm
150
mm) and
column
less than 4.5
samples,
and
require
high throughput
mono and(2di-saccharide
analysisincapabilities.
Rapid
separation
of
the
eight
most
common
biofuel
monoand
disaccharides
can
be
minutes
and (b)
the CarboPac
SA10-4μm
(2 × 250
mm) column
less than 6 fast,
minutes
The
CarboPac
SA10-4μm
column
is specifically
designed
for the in
exceptionally
achieved
onFigure
the (a)1.CarboPac
SA10-Fast-4μm
(2 ×eight
150 most
mm) column
infood
less sugars
than 4.5
as
shown in
capability
to analyze
high-resolution
analysisThe
of carbohydrate
samplesthe
for biofuel
andcommon
food applications.
minutes
andseparation
(b) the CarboPac
SA10-4μm
× 250
mm) columnThe
in less
than 6 SA10minutes
with
aseparation
short
time ismost
important
for(2
many
applications.
CarboPac
Rapid
of the eight
common
biofuel
mono- and disaccharides
can be
as showncolumn
in Figure 1. separate
The capability
to analyze
the eightin
most common
foodassugars
Fast-4μm
six common
food
than in
8 less
min,
achieved
on the (a)can
CarboPac SA10-Fast-4μm
(2 ×sugars
150 mm)less
column
thanshown
4.5
a short
separation time is important for many applications. The CarboPac SA10inwith
Figure
2. (b)
minutes
and
the CarboPac SA10-4μm (2 × 250 mm) column in less than 6 minutes
Fast-4μm column can separate six common food sugars in less than 8 min, as shown
as shown in Figure 1. The capability to analyze the eight most common food sugars
in Figure 2.
with a short separation time is important for many applications. The CarboPac SA10The CarboPac
SA10-4μm
column
capablefood
of analyzing
Fast-4μm
column
can separate
six is
common
sugars insamples
less thancontaining
8 min, as analytes
shown
a broad
of sample concentrations. Packed with highly porous resin beads,
inover
Figure
2. range
The
CarboPac
SA10-4μm
column
is
capable
of
analyzing
samples
containing
analytes
the CarboPac SA10-4μm column has significantly higher capacity than other Dionex
over a broad
rangeand
of sample
concentrations.
with highly porous
resin
CarboPac
columns
is the column
of choicePacked
for high-concentration
mono
andbeads,
dithe CarboPac
SA10-4μm
column
significantly
higher
capacity than other Dionex
saccharide
sample
analysis.
Whenishas
the
targetofsugars
at high-concentrations
The
CarboPac
SA10-4μm
column
capable
analyzing
samples containingare
analytes
CarboPacon
columns
andCarboPac
is the column
of choice
for high-concentration
mono and diseparated
a Dionex
SA10-4μm
column,
linearity
is maintained
over
a broad range
of sample
concentrations.
Packed with
highly
porous resinand
beads,
saccharide
sample analysis.
When
the target
sugars
at high-concentrations
are of
accurate
quantitative
analysis
is achieved.
Figure
3 demonstrates
the separation
the
CarboPac
SA10-4μm
column
has significantly
higher
capacity than
other Dionex
separated
on
a
Dionex
CarboPac
SA10-4μm
column,
linearity
is
maintained
and
five sugarscolumns
in a highand
concentration
corn
stover hydrolysate
sample on mono
the CarboPac
CarboPac
is the column
of choice
for high-concentration
and diaccurate quantitative
analysis
is achieved.
Figure
3 demonstrates
theobtained
separation
of
SA10-4μm
2 mm column.
Excellent
separation
of biofuel
sugars was
saccharide
sample
analysis.
When the
target sugars
at high-concentrations
arewhen
fiveg/L
sugars
in a high
concentration
corn
stover
hydrolysate1:200
sample
on the
CarboPac
43
corn
hydrolysate
sample
was
injected
dilution
of the
separated
onstover
a Dionex
CarboPac
SA10-4μm
column,using
linearity is maintained
andsample
SA10-4μm
2 mm column. Excellent
of biofuel
was (15
obtained
and
electrochemical
flow
cellseparation
withFigure
optimum
gasketsugars
thickness
mil forwhen
the
accurate
quantitative detector
analysis is
achieved.
3 demonstrates
the separation
of 2
43 g/L
corn or
stovermil
hydrolysate
sample
was injected using 1:200 dilution of the sample
mm
column
for the 4 mm
column).
five
sugars
in a 62
high concentration
corn
stover hydrolysate sample on the CarboPac
and electrochemical detector flow cell with optimum gasket thickness (15 mil for the 2
SA10-4μm 2 mm column. Excellent separation of biofuel sugars was obtained when
mm column or 62 mil for the 4 mm column).
43 g/L corn stover hydrolysate sample was injected using 1:200 dilution of the sample
and electrochemical detector flow cell with optimum gasket thickness (15 mil for the 2
mm column or 62 mil for the 4 mm column).
FF
Fa
F
F
FFi
Fas
F
(
F
(2
T
e
T
b
th
R
E
H
.
Figure 3. High-resolution separation of sugars in a high concentration corn
Figure 3. High-resolution separation of sugars in a high concentration corn
stover hydrolysate sample using a Dionex CarboPac SA10-4μm 2 mm column
stover hydrolysate sample using a Dionex CarboPac SA10-4μm 2 mm column
and 15 mil gasket thickness.
and 15 mil gasket thickness.
ex
P
e
d
g
co
ca
o
o
w
d
p
w
e
Ta
Ta
When used with the on-line electrolytic eluent generator (EG) which produces highly
When used with the on-line electrolytic eluent generator (EG) which produces highly
reproducible,carbonate-free hydroxide eluents, CarboPac SA10-4μm column can be
reproducible,carbonate-free hydroxide eluents, CarboPac SA10-4μm column can be
used to provide highly reproducible chromatographic separation of target analytes.
used to provide highly reproducible chromatographic separation of target analytes.
Figure 4 shows the consistent separation results obtained after 1000 injections of a
Figure 4 shows the consistent separation results obtained after 1000 injections of a
biofuel sugar sample using a Dionex CarboPac SA10-4μm column.
biofuel sugar sample using a Dionex CarboPac SA10-4μm column.
e
FFigure
1. 1.
Fast
separation
of of
biofuel
sugars
onon
thethe
(a)(a)
Dionex
CarboPac
SA10FFigure
Fast
separation
biofuel
sugars
Dionex
CarboPac
SA10Fast-4μm
(2 (2
× 150
mm)
and
(b)(b)
Dionex
CarboPac
SA10-4μm
(2 (2
× 250
mm)
Fast-4μm
× 150
mm)
and
Dionex
CarboPac
SA10-4μm
× 250
mm)
s
C
Th
pe
di
C
re
SA
an
be
s
•F
e
•H
•H
Figure 2. Fast separation of food sugars on a Dionex CarboPac SA10-Fast-4μm
Figure 2. Fast separation of food sugars on a Dionex CarboPac SA10-Fast-4μm
(2 × 150 mm) column.
(2 × 150 mm) column.
Figure 4. Consistent separation results obtained after 1000 separations of a
Figure 4. Consistent separation results obtained after 1000 separations of a
biofuel sugar sample using a Dionex CarboPac SA10-4μm column.
biofuel sugar sample using a Dionex CarboPac SA10-4μm column.
2 A New Anion Exchange Column for Fast Ion Chromatographic Separation of Monosaccharides and Disaccharides in Biofuel, Food, and Beverage Samples
•H
•S
The CarboPac SA10-4μm column is compatible with KOH eluents prepared by
electrolytic eluent generator only. It is not compatible with manually prepared eluents.
The smaller particles used in the CarboPac SA10-4μm column generate higher
backpressure under standard operating conditions. Therefore, it is recommended that
the CarboPac SA10-4μm column be used with a Thermo Scientific™ Dionex™
Reagent-Free™ HPIC System equipped with an Eluent Generator and
Electrochemical Detector, such as the Thermo Scientific™ Dionex™ ICS-5000+
HPIC™ System or Thermo Scientific™ Dionex™ Integrion HPIC™ System.
Pulsed amperometric detection (PAD) permits detection of carbohydrates with
excellent signal-to-noise ratios down to approximately 10 picomoles without requiring
derivitization. Carbohydrates are detected by measuring the electrical current
generated by their oxidation at the surface of a gold electrode. A gold electrode is
commonly used for determination of monosaccharides and disaccharides. Although
carbohydrates can be oxidized at a gold working electrode, over time the products of
oxidation reactions can foul the surface of the electrode. Electrode fouling with
oxidation byproducts or sample components will have reduced response, requiring
Figure
Consistent
separation
results
obtained
after 1000
separations
of a
working4.electrode
polishing
to restore
the surface.
Thermo
Scientific™
Dionex™
biofuel
sugar
using
a Dionex
SA10-4μm
column. (i.e.
disposable
goldsample
electrodes
eliminate
theCarboPac
need for electrode
reconditioning
polishing). Disposable electrodes are economical, and thus can be replaced frequently
while delivering peak area reproducibility superior to conventional Au working
electrodes. The peak area reproducibility for five disposabl eelectrodes is shown in
Table 2.
Conclusion
The Thermo Scientific™ Dionex™ CarboPac™ SA10-4μm columns are high
performance anion-exchange columns that provide optimal separation of mono- and
disaccharides. Compared to the previous Dionex CarboPac SA10 columns, the new
CarboPac SA10-4μm columns use smaller resin particles for more efficient separations
resulting in more accurate peak integration and more reliable results. The CarboPac
SA10-Fast-4μm columns are shorter anion-exchange columns for the fast separation
and accurate quantification of mono- and disaccharides in biofuels, foods and
beverages. These columns provide:
• Fast analysis and short separation times
• High resolution
• High capacity
• High reproducibility
• Superior peak efficiency
Table 1. Specifications of DionexTM CarboPacTM SA10-4µm columns
Conclusion
The Thermo Scientific™ Dionex™ CarboPac™ SA10-4μm columns are high
performance anion-exchange columns that provide optimal separation of mono- and
disaccharides. Compared to the previous Dionex CarboPac SA10 columns, the new
CarboPac SA10-4μm columns use smaller resin particles for more efficient separations
resulting in more accurate peak integration and more reliable results. The CarboPac
SA10-Fast-4μm columns are shorter anion-exchange columns for the fast separation
and accurate quantification of mono- and disaccharides in biofuels, foods and
beverages. These columns provide:
• Fast analysis and short separation times
• High resolution
• High capacity
• High reproducibility
• Superior peak efficiency
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