Development and Comparisons of Various ELISA for
the Diagnosis of Foot and Mouth Disease Virus (FMDV)
Sumita Bose, Shela Aman, and Masarrat Ali*
Alpha Diagnostic Intl. Inc.,
San Antonio, Texas, USA
www.4adi.com
Arsh Bio
FMDV Vaccines (Whole Virus/Inactivated)
• Vaccine status with anti-VP1 ELISA
• ELISA not quantitative
• Vaccines contaminated with NSPs
• DIVA tests (3ABC) ELISA not reliable
• Assess various NSPs ELISA
• 2ABC, 3AB3, 3ABC, 3D ELISA
FMD Vaccine (inactivated, O, A, A1) – BioFMD, India generate antiVP1 to O, A, A1, SAT1-2 and NSPs in rabbits
Antibodies to O, A, A1,
and also SAT1, SAT2 VP1s
Antibodies to 2ABC and 3ABC NSPs
making DIVA Test difficult
Natural Infection with FMD Virus (O, A) produced
cross-reactive VP1 antibodies in bovine
Bovine Infected with
FMD O (28 dpi)
Bovine Infected with
FMD A (28 dpi)
FMDV Antibodies in random Bovine Samples
Sample Analyses
1 = Negative
2 = Natural Infection (DIVA)
3 = Vaccinated/Infected
4 = Vaccinated
Arsh Biotech. India
2nd Generation Quantitative ELISA kits Developed
Quantitative Antibody ELISA Kits
FMDVP1 IgG:
NSPs:
Serotypes O, A, A1, SAT1-3
2ABC, 3AB3, 3ABC, and 3D
Quantitative Antigen ELISA Kits
VP1 Proteins:
NSPs:
Serotypes O, A, A1, SAT1-3
2ABC, 3AB3, 3ABC, and 3D
ELISA Kits:
• 105 mins at RT (60+30+15), all reagents (Antigen or antibody controls), buffers,
sample diluent supplied in ready to use stable liquid format.
• Sensitivity ~<1 ng/ml of antigen or antibody
• Stability ~ 1 year
Natural Infection with FMD Virus (A1, SAT1) produced
cross-reactive VP1 antibodies in bovine
Bovine Infected with
FMD A1 (28 dpi)
Bovine Infected with
FMD SAT1 (42 dpi)
Natural Infection with FMD Virus (SAT2-3) produced
cross-reactive VP1 antibodies in bovine
Bovine Infected with
FMD SAT2 (28 dpi)
Bovine Infected with FMD
SAT3 (28 dpi)