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Your partner for
culture media
bioMérieux S.A.
69280 Marcy l’Etoile
France
Tel. : 33 (0)4 78 87 20 00
Fax : 33 (0)4 78 87 20 90
www.biomerieux.com
www.biomerieux-diagnostics.com
Contents
4 2 Committed to Meeting Your Challenges
15 2 Product Insight -
Range and Culture Media
TM
44 2 Automated Specimen Inoculation - Previ Isola
46 2 Specimen Protocols Guidelines “From Specimen to Plate”
2
Committed to Meeting
Your Challenges
4 2 Committed to Meeting Your Challenges
5 2 Expertise & Innovation
6 2 International Vision - Local Proximity
8 2 Our Quality Assurance Commitment - Making Your Daily Life Easier
10 2 Complete Product Traceability - Making Your Daily Life Easier
12 2 Environmental Protection
13 2 Shelf-life and Temperature Storage
14 2 Customer Service
3 Committed to Meeting
Your Challenges
Laboratories are more than ever a pivotal role in ensuring
patients receive the most appropriate healthcare
rapidly given the current worldwide context.
• Hospitals are faced with healthcare-associated infections that are
recognized worldwide as a major public health issue. They need
effective microbiology solutions to help with the prevention,
intervention and surveillance in the fight against HealthcareAssociated Infections (HAI).
• Management of healthcare expenditure implies having the means
to ensure improved patient triage, better discerned use of antibiotics,
freeing up patient beds quicker etc…
• Regulatory requirements are ever present and form an integral
part of patient safety.
• Time to result is increasingly important for many diseases; this
means having a linear workflow right from sample management
and colony isolation to result print out.
4
As a key part of this healthcare picture, you as a laboratory need
to have confidence in the microbiology solutions you chose to
address these daily challenges. This is why we are committed
to offering you an extensive range of cost-effective, nutrient pre-plated
media. They are technically sophisticated to help ensure you obtain
easy-to-read, rapid results, whatever the quantity and type of microorganism in the specimen.
bioMérieux
Expertise & Innovation
With over 45 years experience in the field of microbiology,
bioMérieux has unique know-how and expertise in microbial culture,
enumeration, detection, identification and antimicrobial resistance
testing.
Rapidity of diagnosis and accuracy of treatment impact clinical
outcome. This is why bioMérieux proposes an extensive range of
quality media to ensure you have reliable, cost-effective
microbiological solutions to empower clinical decisions.
With your specific daily challenges in mind, we used our recognized
expertise to create an innovative range of chromogenic media,
chromID™, to provide you with the simultaneous culture and
identification of microorganisms you need. Part of this exclusive
range covers the screening of Multi-Drug Resistant Organisms to
help in the fight against HAI.
To help optimize your daily workflow, we now offer an automated
plate inoculation system, PREVI™ Isola. This instrument standardizes
plate inoculation and results while maximizing bacteria colony
isolation.
bioMérieux is still committed to offering timely, relevant
solutions now and in the future.
Today, with bioMérieux, obtaining quality media solutions
is simpler than ever before.
5 USA
PORTLAND
International Vision
Local Proximity
Our International vision ensures we can anticipate your media
requirements while providing specific local solutions where
needed. We offer worldwide media coverage with just the right
amount of proximity through our strategically placed production sites.
We are closer to our customers than ever before to ensure we meet
your daily needs. There are 3 European production sites (France,
the UK and Spain) - the French site being the largest site in Europe
- and 5 other sites spread around the world in Australia, Brazil, the
U.S.A, and Canada.
bioMérieux has manufactured culture media since 1963 and our
main, referential site in France offers its vast industrial expertise and
know-how to the other production sites. This ensures we have
standardized, innovative products worldwide while being able to
address more local and specific needs. This is the perfect balance
to ensure you have just which media you need exactly when you
need them.
6
CANADA
TORONTO
USA
LOMBARD
UK
BASINGSTOKE
SPAIN
MADRID
FRANCE
CRAPONNE
BRAZIL
RIO DE JANEIRO
AUSTRALIA
BRISBANE
7 Our Quality Assurance
Commitment
Making Your Daily Life Easier
Our objectives are to:
• guarantee you have the highest, reproducible quality
products which comply with the strictest standards
• ensure we actively help you to meet your daily
quality requirements.
To be able to do this, bioMérieux employs a rigourous Quality Assurance
policy for all its products and services. Our Quality System is certified
ISO 9001 version 2000 for all its activities and NF EN ISO 13485
version 2004 for clinical activities, including culture media.
By applying a Quality Assurance system, we ensure the reproducibility
of product performance throughout a product’s life cycle. The main
regulatory guarantee for our products is that they comply with the
Directive 98/79/CE (1998/10/27) relative to medical devices for
in vitro diagnostic use. As such our products must reach strict levels
of security and safety to be able to be commercialized.
8
9 Complete Product Traceability
Making Your Daily Life Easier
As we are committed to providing our clients with high
quality products which increase user and patient safety
while ensuring complete product traceability, we also
follow a number of other standards which are related to
this main directive (see page 11).
To facilitate your regulatory requirements, we also provide
a complete range of documents on-line in our Technical Library:
www.biomerieux.com
v
• Package Inserts
• Non-hazardous Product Statement
• Declaration of Product Conformity Statement
• Thermal Shock Statement
• Quality Control Certificates
10
WHAT D O E S B I O M É R I E U X
P R OV I D E TH E U S E R A S P R O O F
O F P R O D U C T Q UALIT Y ?
PA C K A G E I N S E R T S
WHAT D O E S IT COVE R ?
STAN DAR D FO LLOWE D
Clear Performance Details are given in the package
NF EN 13612 "Performance evaluation of in vitro diagnostic medical
devices" (September 2002)
insert of each medium.
This data comes from bioMérieux's internal studies and
external performance evaluation studies (performed with
routine specimens).
This standard concerns requirements relating to the, management, assessment and
documentation of manufacturer performance evaluations.
QUALIT Y CONTROL
C E R T I F I C AT E S
The Quality Control Certificate is available for
each Lot: bioMérieux provides performance criteria and
NF EN 12322 "Culture media for microbiology / Performance criteria for
culture media" (July 1999)
the collection strains used for Quality Control of culture
media in the certificate.
This standard concerns performance of culture media used in microbiology labs
(traceability, compatibility, reproducibility of product performance throughout the
product’s life cycle and suitability.
KIT LABELING,
T H E R M A L S H O C K S TAT E M E N T ,
PA C K A G E I N S E R T S
The Shelf-Life is determined from Stability Studies:
NF EN 13640 "Stability testing of in vitro diagnostic reagents" (August 2002)
bioMérieux indicates the expiration date on each kit label
while storage conditions before first, and after first opening
are indicated in the package insert.
Temperature storage is also indicated on kit labeling
(see page 13).
This standard concerns requirements for the stability of in vitro diagnostic reagents:
gives specific requirement for real-time testing when generating stability data
(e.g. transport determination of stability after opening.
Clear and Detailed Labeling Information: given on
each product's label and package insert.
Guarantee of Compliance with the EC Regulations:
bioMérieux gives this information on each product's label
and package insert.
Clear and Easy Symbols for all Users: given on each
product's label and package insert.
Symbols reduce the need for multiple translation in local
languages, simplify labeling, prevent the creation of different
symbols conveying the same information.
NF EN 375 "Information provided by the manufacturer with in vitro
diagnostic reagents for professional use" (April 2001)
This standard concerns information supplied by the manufacturer of in vitro
diagnostic reagents including reagents, calibrators, control material and kits.
It defines the information which must be indicated on labels and instructions for use
of each IVD product.
NF EN 1041 "Information supplied by the manufacturer with medical
devices" (October 2008)
This standard concerns the information to be provided by the manufacturer for
different types of medical devices covered by the EU Directives.
NF EN 980 "Graphical symbols for use in the labelling of medical devices"
(July 2008)
This standard concerns the graphical symbols to be used on information provided by
the manufacturer for medical devices.
ISO 15223 "Symbols to be used with medical device labels, labeling, and
information to be supplied + amendments" (April 2000)
This standard concerns symbols conventionally used to convey information essential
for the correct and effective use, safe and effective use of medical devices.
PA C K A G I N G
Environmental Protection: bioMérieux confirms its
Directive 94/62/CE "Packaging and packaging waste" (December 1994)
environmental commitment with: recycled and recyclable
cardboard, reduce waste volume and weight (see page 12).
This standard concerns the harmonization of the various national measures relative
to packaging and packaging waste to avoid/reduce the effect on the environment.
This is to offer maximum environmental protection and ensure the free circulation between
member countries of goods following this standard.
11 Environmental Protection
In addition to following the European directive 94/62/EC “Packaging
and packaging waste, one of bioMérieux’s guiding Principles is
"We belong to a community". It is only natural that we embrace a
responsible attitude toward our environment in all aspects of our
business worldwide as regards energy, water, paper, waste, emissions.
As part of our environmental actions for our media products, we still
use recycled and recyclable cardboard to make our boxes but we
have worked even further on reducing the volume of plated media
packaging. By moving from our already innovative “fold-box”principle
to an ingenious, new, patented fold-lockTM principle (once flattened
the box stays flat) we further facilitate the disposal of pre-plated
culture media boxes and reduce your daily waste volume and
weight. Not only do we actively try to contribute to saving our
environment but we help you to do the same.
12
25°C
Shelf-life and Temperature Storage
15°C
In addition to following the regulation of “Stability testing of in vitro diagnostic reagents”
we also validate the “use” of 4-week storage at 15-25°C.
This is to ensure we offer maximum flexibility in the daily work routine of laboratory technicians.
Temperature Storage Validation
The shelf-life of bioMerieux's culture media
is determined by stability study results.
These studies are performed in real-time.
During this stability study two kinds of thermal
shocks are simultaneously applied on the
media :
• Simulation of a break in the cold chain :
Thermal shocks are test temperature cycles
to which products may be subjected during
production or transportation.
• 4 weeks at 15-25°C.
The stability study consists of checking
the conformity of appearance, pH, and
bacteriological activity.
This information is included in the Thermal
Shock Statement which provides you with
proof of our validation process.
Temperature
Thermal shocks including 4 weeks at 15-25°C
Storage
temperature
Time
Control conform
Control not conform
Shelf-life
2 Example of a thermal shock protocol for preplated media:
Storage: 2-8°C
10-15°C for 4 weeks
18-25°C for 6 days, including 35-39°C for 8 hours
15-25°C for 4 weeks
2-8°C
13 Customer Service
Our customers deserve only the very best and
as such bioMérieux is committed to continuously
improving the quality of products and services
we deliver you.
Our Customer Service meets this challenge by offering
a wide range of services: rapid responses to customers'
questions and complaints, tailor-made customer
product training courses, proactive and curative system
maintenance and even workflow auditing.
To ensure you receive first class service regardless of your
geographical location, local bioMérieux technical assistants
specialized in microbiology are ready to help you backed up by
the expertise of the global customer service support team. This
organization provides a winning combination of local proximity
with worldwide, up-to-date knowledge to ensure our customers
have the expertise and follow-up they require when needed.
14
Product Insight
17 2
Chromogenic Media for
Simultaneous Isolation and Immediate Identification
23 2
Chromogenic Media for
the Screening of Multidrug Resistant Organisms
25 2 Blood Agar: Complex, Selective and Differential Media
29 2 Specific Media: Selective Isolation for Specific Organisms
38 2 Simple Media: Isolation of Organisms
41 2 Media for Susceptibility Testing
42 2 Media for Environmental Controls: Surfaces, Air, Water
For more details see technical sheets
15 An extensive range
of chromogenic media
for the simultaneous
culture and identification
of microorganisms
16
With chromIDTM bioMérieux offers you an extensive range of
chromogenic media for the simultaneous culture and identification
of microorganisms.
Designed to meet your increasing daily challenges, these high quality
media provide you with reliable results and true peace-of-mind.
Our recognised expertise in culture, identification and susceptiblity
testing naturally led us to pioneer the chromogenic media concept
back in 1989. Since then, we have continued to create innovative
chromogenic solutions to meet your everyday needs.
2CHROMOGENIC
M E D I A F O R S I M U LTA N E O U S I S O L AT I O N A N D I M M E D I AT E I D E N T I F I C AT I O N
chromID CPS
TM
chromID CPS
®
2 for the isolation, enumeration and direct identification of E. coli, Proteus
and Enterococci and KESC in one single step using urine specimens
Isolation and enumeration of all urinary tract pathogens.
Colonies are well isolated and easy to identify with real differentiating colours.
Microbial enumeration with colourless background to optimise colony counting.
Direct identification: chromID CPS contains specific substrates of the enzymatic activities to be detected.
Greater Ease-of-use:
• E. coli: ß-glucuronidase (ß-GUR). No indole test required.
• Proteus: spontaneous colouration of colonies producing deaminase.
• Enterococci: ß-glucosidase (ß-GLU).
Greater Reliability: • Nutrient capacity.
• Sensitivity of detection and specificity of colouration for the main
organisms usually found in urine.
chromID CPS
• Identification of the bacterial group KESC (Klebsiella, Enterobacter,
Ref. 43 541 • kit of 20 plates
Serratia and Citrobacter).
Ref. 43 549 • kit of 100 plates
KESC => better colour intensity, due to increased ß-glucosidase activity.
C. albicans
K. pneumoniae
(brownish-green colonies)
and Enterococci
(turquoise colonies)
chromID CPS / Columbia CNA
+ 5% sheep blood
TM
chromID CPS
Columbia CNA
+ 5% sheep
blood
®
Chromogenic medium for the enumeration of organisms in urine specimens and the direct
identification of Escherichia coli, Enterococcus, KESC and Proteeae.
Selective isolation of fastidious bacteria.
Determination of hemolysis.
Incubation: 24 hours
E.coli ATCC 25922,
Proteus mirabilis
ATCC 43071
and
Enterococci ATCC 29212
Incubation: 24 hours
S. epidermis ATCC 14990
S. agalactiæ ATCC 12386
(x 2.5)
chromID CPS /Columbia
CNA + 5% sheep blood
Ref.
Ref.
43 463 • kit of 10 plates
43 473 • kit of 20 plates*
* Consult your local bioMérieux representative for more product information and availability.
17 2CHROMOGENIC
M E D I A F O R S I M U LTA N E O U S I S O L AT I O N A N D I M M E D I AT E I D E N T I F I C AT I O N
chromID
Candida
chromID Candida
Incubation: 24 hours
C. albicans ATCC 10231,
C. glabrata,
C. krusei ATCC 6250
of Candida albicans
TM
2 for the selective isolation of yeasts and the direct identification
C. albicans
C. krusei
Candida albicans colonies are coloured blue by the specific hydrolysis of a hexosaminidase
chromogenic substrate (bioMérieux patent).
The hydrolysis of a second substrate (pink colouration) differentiates mixed cultures and orients
identification of other species colonies (bioMérieux patent).
Immediate identification of C. albicans in just 24 hours* = Blue colonies
Greater intensity for C. albicans colonies.
• Optimum differentiation of mixed cultures
• Orientation of identification towards Candida (C. tropicalis, C. lusitaniae and C. kefyr) = pink colonies
• White colonies characteristic aspect = orientation towards yeasts or filamentous fungi
chromID Candida
Ref.
Ref.
43 631 • kit of 20 plates
43 639 • kit of 100 plates
chromID Salmonella
TM
chromID
Salmonella
2 for the selective isolation of all Salmonella serotypes
Incubation: 24 hours
S. Typhimurium ATCC 14028
S. Enteritidis,
P. vulgaris
Three chromogenic substrates optimize the selective isolation and differentiation of Salmonella:
• Specific detection of the esterase enzymatic activity on a colourless background =
Pale-pink to mauve colonies for Salmonella (reading between 18 to 24 hrs)
• Differentiation of other bacteria (colonies of a different colour)
• Inhibition of Gram-positive bacteria and yeasts
The nutrient capacity, colour intensity, sensitivity of detection and specificity of colouration,
ensure optimum differentiation of mixed cultures.
chromID Salmonella enables the detection of serotype Typhi, Paratyphi and most Salmonella Lactose +
chromID Salmonella
Ref.
Ref.
18
43 621 • kit of 20 plates
43 629 • kit of 100 plates
* For more details see technical sheets
Selenite F broth
Ref.
42 099 • kit of 20 tubes
Rappaport broth
Ref.
42 091 • kit of 20 tubes
chromID S. aureus
chromID
S. aureus
TM
2 for the the direct identification of S. aureus
Direct identification of S. aureus is based on the spontaneous green colouration of glucosidase-producing
colonies (patent pending).
Greater Rapidity with the immediate identification of S. aureus = Green colonies (reading between
18 and 24 hours).
Greater Reliability:
• Excellent performance for the culture of S. aureus in terms of nutrient capacity, detection sensitivity
and colouration specificity.
• Optimum differentiation of mixed cultures due to the presence of a 2nd substrate.
• Orientation of identification towards Staphylococci*= S. epidermidis (white colonies),
S. saprophyticus (pink colonies), S. xylosus (mauve colonies).
Inhibition of other bacteria (Gram + and Gram -) and yeasts.
Incubation: 24 hours
S. aureus ATCC 25923
S. aureus (green colonies),
S. saprophyticus
(pink colonies),
S. xylosus (mauve colonies)
S. epidermidis
(white colonies)
Slidex® MRSA
chromID S. aureus
Ref.
43 371 • kit of 20 plates
Ref.
73 117 • kit of 50 tests
chromID Strepto B
TM
chromID
Strepto B
2 for the screening of all S. agalactiae
Prevention of Perinatal Group B Streptococcal disease
Incubation: 24 hours
S. agalactiae NCTC 8190
Original Principle
• Three chromogenic substrates to optimise the identification of all gr. B Streptococcus = pale pink
to red colonies which are round and pearly after 18-24 hour incubation*.
• Excellent performance for the GBS prenatal screening in terms of nutrient capacity and sensitivity
of detection.
• Detection of all GBS strains, including non ß-hemolitic strains.
• Differentiation of mixed cultures.
• Selective inhibition of most bacteria not belonging to the species S. agalactiae, as well as yeasts.
Greater Simplicity: Incubation in aerobic conditions.
chromID
Strepto B
Todd Hewitt Broth
+ Antibiotics
SLIDEX
Strepto Plus B
SLIDEX
Strepto Plus
Ref. 43 461:
kit of 20 plates
Ref. 42 116:
kit of 20 tubes of 9 ml
Ref. 58 819:
kit of 50 latex tests
Ref. 58 810:
kit of 50 latex tests
* For more details see technical sheets
Incubation: 24 hours
GBS vaginal specimen
collection
Incubation: 48 hours
GBS vaginal specimen
collection
19 2CHROMOGENIC
M E D I A F O R S I M U LTA N E O U S I S O L AT I O N A N D I M M E D I AT E I D E N T I F I C AT I O N
chromID O157:H7
chromID
O157:H7
Incubation: 24 hours
E. coli O157:H7
ATCC 43890
Citrobacter freundii
ATCC 8090
E. coli ATCC 11229
Proteus mirabilis
ATCC 12453
TM
2 for the selective isolation and presumptive identification of E. coli O157:H7
E. coli O157:H7
Citrobacter - Proteus
Other E. coli
E. coli O157:H7
chromID
Vibrio
chromID O157:H7, an innovative chromogenic medium enabling easy differentiation between
the O157:H7 serotype and other microorganisms:
• identification of E. coli O157:H7 after only 24 hrs of incubation at 37°C
• characteristic green to blue-green colour of E. coli O157:H7 colonies
• more fertile than SMAC medium, and equally selective
• inhibition of Gram-positive bacteria and yeasts
• increase in selectivity with respect to Gram-negative bacteria by adding Cefixime Tellurite,
for highly contaminated samples.
chromID O157:H7
Ref.
cefixime tellurite mixture
42 605 • 6 x 200 ml bottles
Ref.
42 606 • 6 x 4 ml vials
chromID Vibrio*
TM
Incubation: 24 hours
V. cholera
2 for the selective isolation of Vibrio
Vibrio parahaemolyticus
Incubation: 24 hours
Presumptive identification of V. cholerae is based on the blue-green colouration of
beta Galactosidase-producing colonies.
Differentiation of V. parahaemolyticus: Pink colonies based on arabinose assimilation.
chromID Vibrio
Ref.
20
Fecal culture naturally
contaminated by V. cholerae.
Images from Dr Scheftel,
Institut de Bactériologie,
Strasbourg, France.
43 762 • kit of 20 plates
* Consult your local bioMérieux representative for more product information and availability.
chromID P. aeruginosa*
chromID
P. aeruginosa
TM
2 for the direct identification of Pseudomonas aeruginosa
The direct identification of P. aeruginosa is based on the specific pink to violet coloration
of aminopeptidase-producing colonies due to the chromogenic substrate ß-alanyl-resorufamine
(2 bioMérieux patents).
Incubation: 24 hours
P. aeruginosa
ATCC 27853
P. aeruginosa morphotypes
in cystic fibrosis specimens.
chromID P. aeruginosa
Ref.
43 462 • kit of 20 plates
Visualisation
of golden-metallic coloured
P. aeruginosa colonies.
* Consult your local bioMérieux representative for more product information and availability.
21 S C R E E N I N G
O F
M U L T I D R U G -
R E S I S T A N T
O R G A N I S M S
Isolate Colonies
& Start Patient Isolation
You need rapid and easy detection of MDRO* responsible for
the most frequently found Healthcare Associated Infections.
You recognise our expertise in culture, identification and
susceptibility testing. We naturally designed a specific range
of MDRO chromogenic media to meet your everyday needs.
This range will help you:
• Reduce the number of infections by avoiding:
- cross transmission between patients through isolation
- auto-infection of colonized patients.
• Adjust the antibiotic surgical prophylaxis depending on
the patient Multidrug-Resistant Organisms colonization status.
• Optimize the use of isolation beds.
• Control the level of antibiotics resistance.
• Provide healthcare cost-effectiveness.
22
* Multidrug-Resistant Organisms
2CHROMOGENIC
M E D I A F O R T H E S C R E E N I N G O F M U LT I D R U G R E S I S TA N T O R G A N I S M S
chromID MRSA
Incubation: 24 hours
S. aureus ATCC 43300
chromID MRSA
Innovative, specific chromogenic
media range
TM
MRSA nasal specimen
collection.
Image from D. Blanc,
CHUV Lausanne
2 for the direct screening of methicillin-resistant S. aureus (MRSA)
in 18-24 hours*
chromID MRSA is dedicated to the surveillance culture or screening of MRSA to identify hospitalized
patients requiring isolation.
The screening of MRSA carriers with surveillance cultures is a key step in the fight against nosocomial
infections.
chromID MRSA has been developed to answer this major health concern.
MRSA positive specimen
collection.
Image from Dr. C. Nonhoff,
Erasme Hospital, Belgium
Identification of MRSA strains is based on the spontaneous green colouration of
α-glucosidase-producing colonies (patent pending) and the presence of an antibiotic, cefoxitin.
Greater Rapidity:
Extremely easy-to-read identification of MRSA = Green colonies after 18-24 hour incubation.
Greater Reliability:
• Excellent performance for the screening of MRSA in terms of nutrient capacity, detection
sensitivity and colouration specificity.
• The presence of cefoxitin enables the detection of MRSA strains including hetero-resistant strains.
• The selective mixture inhibits most bacteria not belonging to the genus Staphylococcus,
as well as yeasts.
chromID MRSA
Ref.
Ref.
43 451 • kit of 20 plates
43 459 • kit of 100 plates
Enrichment broth
Agglutination Tests
Heart-Brain broth
Ref. 42 081 • kit of 20 tubes
Slidex® Staph Plus
Ref. 73 115 • kit of 50 tests
Ref. 73 116 • kit of 250 tests
Todd-Hewitt broth + antibiotics
Ref. 42 116 • kit of 20 tubes
* For more details see technical sheets
Stronger green coloration
of colonies when plates read
upside down through
the plate bottom
MRSA from a nasal swab
specimen.
Image provided by Sullivan
& Nicolaides Pathology,
Brisbane, Australia
Slidex MRSA Detection
Ref. 73 117 • kit of 50 tests
23 2CHROMOGENIC
M E D I A F O R T H E S C R E E N I N G O F M U LT I D R U G R E S I S TA N T O R G A N I S M S
chromID
ESBL
chromID ESBL
Incubation: 18-24 hours
E. coli CIP 103982,
K. pneumoniae
ATCC 700603,
P. mirabilis
ATCC BAA-896
Enterobacteria (ESBL)
TM
2 for the screening of Extended-Spectrum ß-lactamase producing
Incubation: 24 hours
E. coli CIP 103982
Incubation: 24 hours
K. pneumoniae
ATCC 700603
chromID ESBL agar (patent pending) contains:
• A mixture of antibiotics, including cefpodoxime, enabling the selective growth of ESBL-producing
enterobacteria.
• Two chromogenic substrates and one natural substrate enabling the direct identification of the most
frequently encountered ESBL-producing enterobacteria.
- Escherichia coli: spontaneous coloration (pink to burgundy) of ß-glucuronidase-producing strains
(ß-GUR).
- Klebsiella, Enterobacter, Serratia, Citrobacter (KESC): spontaneous green, brownish-green or blue
colouration of strains expressing a ß-glucosidase (ß-GLU).
- Proteeae (Proteus, Providencia, Morganella): spontaneous dark brown to light brown colouration
of strains expressing a deaminase.
Ref.
43 481 • kit of 20 plates
chromID VRE
chromID VRE
TM
Incubation: 48 hours
E. faecium CCUG 36804,
E. faecalis ATCC 51299
2 for the screening of E. faecium and E. faecalis showing acquired
Vancomycin resistance (VRE)
E. faecalis
VRE feces specimen
collections
chromID VRE (patents pending) contains two chromogenic substrates
(α-Glucosidase & ß-Galactosidase) and Vancomycin (8mg/l) which enable:
• Direct identification of E. faecium and E. faecalis after 24 hour incubation.
• Specific and selective isolation & detection of acquired Vancomycin-Resistant enterococci.
• Characteristic colouration of colonies with: Bluish-green colour = E. faecalis - Violet colour = E. faecium
The selective mixture inhibits:
- enterococci with natural resistance (ie. E. casseliflavus and E. gallinarum…)
- most Gram-negative and Gram-positive bacteria as well as yeasts.
chromID VRE
Ref.
E. faecium
VRE feces specimen
collections
24
Whereas identification to the species or group level is direct,
ESBL production must be confirmed by one or more
additional tests.
chromID ESBL
43 002 • kit of 20 plates
Heart-Brain broth
Ref.
42 081 • kit of 20 tubes
Vancomycin Bio-DiscsTM
Ref. 54 912 • kit of 4 boxes
of 50 x 6mm impregnated discs
with Vancomycin 30µg concentration
2 B LO O D
AGAR: COM P LEX, S E LECTIVE AN D DI F F E R E NTIAL M E DIA
Blood agar
Blood agar are isolation media specially designed to facilitate the growth
of fastidious microorganisms, Gram-positive bacteria and all the species
encountered in clinical specimens.
It contains a peptone mixture particularly adapted to the culture of fastidious microorganisms
(streptococci, Listeria...).
The presence of blood enables hemolysis determination, which is a basic criterion
for the orientation of bacterial identification.
Example of characteristic hemolyses:
Streptococcus pneumoniae: a-hemolysis, with a greenish colouration around the colony.
Streptococcus pyogenes: ß-hemolysis, with a clear zone around or under the colony.
Blood agar can be used to subculture bacterial strains in order to obtain pure cultures.
Columbia agar + 5% sheep blood
Columbia agar
+ 5% sheep
blood
2 Isolation of fastidious bacteria
2 Detection of hemolysis
Columbia agar is an isolation medium specially designed to facilitate the growth of fastidious
microorganisms.
Supplemented with sheep blood, it is highly nutritious and therefore adapted to the culture
of most bacterial species, regardless of their metabolism.
Incubation: 24 hours
S. pneumoniæ ATCC 6305
Incubation: 24 hours
S. pyogenes ATCC 19615
Columbia agar
+ 5% sheep blood
Ref.
Ref.
43 041 • kit of 20 plates
43 049 • kit of 100 plates
25 2 B LO O D
AGAR: COM P LEX, S E LECTIVE AN D DI F F E R E NTIAL M E DIA
Columbia CNA
agar +
5% sheep
blood
Incubation: 48 hours
S. pyogenes
ATCC 19615
Columbia CNA agar
+ 5% sheep blood
Incubation: 48 hours
S. aureus
ATCC 25923
2 Selective isolation of fastidious bacteria
2 Detection of hemolysis
Columbia CNA agar + 5 % sheep blood is a selective isolation medium which enables the growth
of Gram-positive bacteria commonly encountered in clinical specimens.
Most Gram-negative bacteria and Bacillus are inhibited by the nalidixic acid and colimycin in the agar.
Columbia CNA agar
+ 5% sheep blood
Ref.
Ref.
Columbia agar + 5% horse blood
Columbia agar
+ 5% horse
blood
2 Isolation of fastidious bacteria
2 Detection of hemolysis
Incubation: 24 hours
L. monocytogenes
ATCC 35152
Columbia agar + 5% horse blood is an isolation medium which has been developed to facilitate
the growth of fastidious microorganisms.
Incubation: 48 hours
S. pyogenes ATCC 19615 +
S. pneumoniæ ATCC 49619
(x 2.5)
Columbia agar
+ 5% horse blood
Ref.
Ref.
26
43 071 • kit of 20 plates
43 079 • kit of 100 plates
43 050 • kit of 20 plates
43 059 • kit of 100 plates
Trypcase Soy Agar
+ 5% sheep blood
Trypcase Soy
Agar
+ 5% sheep
blood
2 Isolation of bacteria
2 Determination of hemolysis
Incubation: 48 hours
S. pyogenes
ATCC 19615 +
S. pneumoniæ
ATCC 63605
Trypcase soy agar + 5% sheep blood is an isolation medium which favours the growth
of all the species currently found in clinical specimens.
Trypcase soy agar
+ 5% sheep blood
Ref.
Ref.
43 001 • kit of 20 plates
43 009 • kit of 100 plates
Chocolate agar + PolyViteX
Chocolate agar
+ PolyViteX
TM
2 Isolation of fastidious bacteria
Chocolate agar + PolyViteX is an isolation medium particularly recommended for the growth of
fastidious strains belonging to the genera Neisseria, Haemophilus, and Streptococcus pneumoniae
encountered in clinical specimens.
This medium is composed of a nutrient base enriched with factors X (hemin) and V (NAD) provided
by the hemoglobin and PolyViteX.
This medium can be used to subculture bacterial strains in order to obtain pure cultures.
Incubation: 48 hours
N. gonorrhoeæ ATCC 43069
Incubation: 24 hours
N. meningitidis ATCC 13090
Storage: shelf-life includes 4 week storage at 15°-25°C.
Chocolate agar + PolyViteX
Ref.
Ref.
43 101 • kit of 20 plates
43 109 • kit of 100 plates
Incubation: 48 hours
N. gonorrhoeæ ATCC 43069,
S. pneumoniæ ATCC 6305
27 2 B LO O D
AGAR: COM P LEX, S E LECTIVE AN D DI F F E R E NTIAL M E DIA
Chocolate agar
+ PolyViteX
VCAT3
agar
Incubation: 48 hours
N. meningitidis
ATCC 13090
Chocolate agar
+ PolyViteX VCAT3 agar
TM
N. gonorrhoeæ
ATCC 43069
2 Selective isolation of Neisseria gonorrhoeae & Neisseria meningitidis
Chocolate agar + PolyViteX VCAT3 agar is a selective medium for the isolation of Neisseria
gonorrhoeae & Neisseria meningitidis from polymicrobial specimens (urogenital, oropharyngeal,
CSF, blood cultures...).
The medium is composed of a nutrient base enriched with factors X (hemin) and V (NAD)
provided by hemoglobin and PolyViteX. The enhanced selectivity of the medium is obtained by
the combination of antimicrobial and antifungal agents.
Storage: shelf-life includes 4 week storage at 15°-25°C.
Chocolate agar
+ PolyViteX VCAT3
Ref.
Haemophilus
Chocolate 2
agar
Incubation: 24 hours
H. influenzae ATCC 10211
43 611 • kit of 20 plates
Haemophilus Chocolate 2 agar
2 Selective isolation of Haemophilus
Haemophilus Chocolate 2 agar is a selective medium for the isolation of the different Haemophilus
speciesfrom polymicrobial specimens of human origin.
This new formula is composed of a nutritive base enriched with factors X (hemin) and V (NAD)
provided by hemoglobin and PolyViteX.
Isolation of Haemophilus species collected from the respiratory or genital tracts is often difficult
due to the presence of large amounts of associated flora.
The selectivity of the media is obtained by the combination of antimicrobial and antifungal agents.
Haemophilus
Chocolate 2 agar
Ref.
Ref.
28
43 681 • kit of 20 plates
43 689 • kit of 100 plates
2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Prevention of Perinatal Group B Streptococcal disease
2 GRANADA media for the screening of S. agalactiae
Granada agar
Incubation: 24 hours
S. agalactiae
ATCC 12386
The original principle - “Granada formulae are selective and differential media that
enable the detection and identification of GBS in clinical specimens. There are 100%
specific for GBS. They use Granadaene to differentiate GBS from other pathogenic
bacteria. The methotrexate, included in the formulae, increases pigment production.”
Dr Manuel de la Rosa, creator of the Granada media.
Granada agar
2 Selective agar for the screening and direct identification of S. agalactiae
Granada agar
Ref.
43 712 • kit of 20 plates
Incubation: 24 hours
Streptococcus B
(red to orange characteristic
colonies)
& Enterococcus faecalis
Todd-Hewitt broth + antibiotics
Ref.
42 116 • Kit of 20 x 9 ml tubes
Granada
Biphasic broth
Granada Biphasic broth
2 Selective broth for the screening and direct identification of S. agalactiae
Granada biphasic broth enables in a single test: Direct specimen inoculation - Culture Identification
Highly Economical: it provides significant time and workload savings
Total Flexibility:
• the tube can be used both in labs or for delocalised patient sampling and inoculation followed
by immediate incubation of GBS within 18-24 hours
• storage: 2-year shelf-life at room temperature
• incubation takes place in an aerobic atmosphere
Granada Biphasic Broth
API® suspension MEDIUM
PSIpettes
Ref. 42
Ref. 70 640 • Kit of 100 ampoules
(3 ml)
Ref. 70
722 • kit of 40 tubes
Incubation: 24 hours
S. agalactiae
ATCC 12386
250 • Kit of 400 units
(5 ml)
29 2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Sabouraud Gentamicin
Chloramphenicol 2 agar
Sabouraud
Gentamicin
Chloramphenicol 2
agar
2 Selective isolation of yeasts and moulds
Sabouraud Gentamicin Chloramphenicol 2 agar is a selective medium recommended for the isolation
of yeasts and moulds from polymicrobial specimens.
The new formula has increased nutrient capacity, colour intensity, sensitivity of detection and
specificity of colouration for fungi.
The increased level of peptones and dextrose favours the growth of fungal strains.
The presence of gentamicin inhibits most Gram-negative and Gram-positive bacteria.
The presence of chloramphenicol improves the selectivity for certain species which may be resistant
to gentamicin (streptococci, Proteus, etc.).
The pH of the agar which is slightly acidic, favours fungal growth more than bacterial growth.
Incubation: 24 hours
C. albicans ATCC 10231
Sabouraud Gentamicin
Chloramphenicol 2 agar
Ref.
Ref.
43 651 • kit of 20 plates
43 659 • kit of 100 plates
Dermatophytes agar*
Ref.
43 062 • kit of 20 plates
Incubation: 72 hours
A. niger ATCC 16404
Incubation: 72 hours
Aspergillus flavus
Incubation: 72 hours
Aspergillus nidulans
30
* Consult your local bioMérieux representative for further product information and availability.
Campylosel agar
Campylosel
agar
2 Selective isolation of Campylobacter
Incubation: 48 hours
Campylo jejuni
ATCC 33291
Campylosel agar is a selective medium for the isolation of intestinal Campylobacter
(C. jejuni and C. coli principally) from stools.
The presence of sheep blood favours the growth of the target species.
The nutrient capacity is enhanced by the use of reducing agents.
Good selectivity is ensured by the antibiotic and antifungal agents present in the medium which
inhibit the majority of bacterial and fungal contaminants.
Characteristic Campylobacter colonies are small and greyish, and sometimes spread along
the inoculation streaks.
Campylosel agar
Ref.
43 361 • kit of 20 plates
A7 Mycoplasma agar
A7
Mycoplasma
agar
2 Selective culture and enumeration of urogenital mycoplasma
A7 Mycoplasma agar is a selective medium for the culture of mycoplasmas, Ureaplasma spp.
and Mycoplasma hominis from urogenital specimens. The Ureaplasma urealyticum species has been
separated into two new species called Ureaplasma parvum and Ureaplasma urealyticum.
For the use of this medium, they should both be considered as Ureaplasma spp.
This medium combines a rich nutritive base containing peptones, horse serum and growth factors
favouring the growth of mycoplasmas (cysteine, PoliViteXTM, arginine, urea).
The appearance of the mycoplasma colonies is as follows:
• Ureaplasma spp.
“Sea urchin” colonies: round colonies surrounded by a brownish-black precipitate (due to oxidation
of manganese sulfate).
• Mycoplasma hominis
“Fried egg” colonies: round colonies with a protruding center surrounded by a lighter halo.
An antibiotic mixture inhibits the growth of most Gram-positive and Gram-negative bacteria
contained in the specimen.
A7 Mycoplasma agar
Ref.
43 003 • kit of 10 plates
Urea-Arginine LYO 2
Ref.
42 508 • kit for 25 tests
Mycoplasma hominis
INVERTED MICROSCOPE (OBJECTIVE X10)
Ureaplasma urealyticum
31 2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
SMAC CT agar
Incubation: 24 hours
E. coli O157:H7 ATCC 43894
SMAC CT agar
2 Selective isolation of Escherichia coli O157:H7
The SMAC CT agar (Mac Conkey with Sorbitol) enables the detection and differentiation of
enterohemorrhagic Escherichia coli of serotype O157:H7, responsible for gastro-intestinal infections
or diseases.
According to epidemiological data, these types of pathology may also be induced
by the O157:H- serotype (non-motile bacteria unlike the O157:H7 serotype).
The presence of sorbitol and tellurite enables the differentiation of E. coli O157:H7 which is
characterized by colourless colonies with a brown centre. E. coli 0157:H7 strains might also generate
colourless colonies with a brown centre.
The other E. coli fermenting sorbitol give pink to red colonies.
The selectivity for Gram-positive bacteria and certain Enterobacteriaceae is ensured by cefixime,
bile salts, crystal violet and tellurite.
SMAC agar
Ref.
BCSA agar
Incubation: 48 hours
B. cepacia ATCC 25416
43 391 • kit of 20 plates
BCSA agar
2 Selective isolation of Burkholderia cepacia
BCSA agar (Burkholderia cepacia Selective Agar) is a medium recommended for the selective
isolation and detection of the species Burkholderia cepacia complexe from clinical specimens of
mainly respiratory origin (sputum and bronchoalveolar washing) in patients suffering from cystic
fibrosis.
The presence of a peptone base and sugars enable optimum growth of the microorganisms.
Crystal violet and the antibiotics present in the medium inhibit most microbial species
(inhibition of Pseudomonas species).
A high volume of agar per plate enables good performance for culture at 72 hours.
BCSA agar
Ref.
32
33 631 • kit of 20 plates
Cetrimide agar
Cetrimide
agar
2 Selective isolation of Pseudomonas aeruginosa
Cetrimide agar is a selective isolation medium for the detection of Pseudomonas aeruginosa
from specimens of various origin.
Cetrimide agar stimulates the production of pyocyanin and the fluorescence of P. aeruginosa.
Its formula is an optimization of King’s A medium: the addition of a quaternary ammonium inhibits
most other microorganisms.
Characteristic colonies present a spontaneous pale green pigmentation and green fluorescence
under ultraviolet light.
Incubation: 24 hours
P. aeruginosa ATCC 10145
P. aeruginosa
ATCC 10145, UV light
Cetrimide agar
Ref.
43 565 • kit of 20 plates
D-Coccosel agar
2 Selective isolation of enterococci and group D streptococci
D-Coccosel
agar
Incubation: 24 hours
E. faecalis ATCC 29212
D-Coccosel agar (Bile Esculin Agar) is used for the selective isolation and differentiation of
enterococci and group D streptococci from polymicrobial specimens.
The characteristic colonies of enterococci and group D streptococci are colourless or grey and
surrounded by a black halo (hydrolysis of esculin).
The selectivity of the medium for Gram-negative bacteria is provided by sodium azide.
Bile inhibits certain Gram-positive bacteria other than enterococci.
D-Coccosel agar
Ref.
43 151 • kit of 20 plates
33 2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Legionella GVPC agar
Legionella
GVPC agar
2 Selective isolation of Legionella
Incubation: 5 days
Legionella pneumophila
Legionella pneumophila
(x 2.5)
Legionella GVPC agar has been specially developed for the isolation from respiratory specimens of
most Legionella species, notably those responsible for infections: Legionella pneumophila, which is
the species most frequently involved (Pontiac fever) but also L. bozemanii, L. longbeachae, L. dumoffii,
L. jordanis, L. gormanii, L. anisa and L. micdadei.
This selective medium also enables the enumeration of Legionella in water according to standards
T90-431 and ISO 11731.
The activated charcoal absorbs any toxic substances in the yeast extract and stimulates the growth of Legionella.
The ACES buffer stabilizes the pH of the medium at 6.9, which is the optimal pH for growth.
The characteristic colonies of Legionella are grey-blue in colour but which can become off-white with age.
They have a regular pinkish edge and, when observed under a binocular microscope, have a ground
glass appearance.
GVPC medium provides excellent inhibition of growth of Gram-positive bacteria, most Gram-negative
bacteria, yeasts and moulds, through an optimized combination of three antibiotics.
Legionella GVPC agar
Ref.
43 031 • kit of 20 plates
Slidex® Legionella Kit
Ref.
73 120 • kit of 50 tests
(not CE marked)
Gardnerella
agar
Incubation: 24 hours
G. vaginalis ATCC 14018
Gardnerella agar
2 Selective isolation of Gardnerella vaginalis
Gardnerella agar is a selective isolation medium for the detection of Gardnerella vaginalis in genital
specimens.
Gardnerella vaginalis, alone or associated with certain anaerobic bacteria (Mobiluncus, Bacteroides
and Prevotella), is responsible for various urogenital infections.
The presence of human blood favours the growth of the species being tested for and produces
ß-hemolysis around the colonies. ß-hemolysis obtained solely on human blood agar is highly
indicative of G. vaginalis.
The antibiotics included in the medium inhibit most Gram-negative contaminants and yeasts.
Gardnerella agar
Ref.
Ref.
34
43 411 • kit of 20 plates
43 039 • kit of 100 plates
Yersinia CIN agar
Yersinia CIN
agar
2 Selective isolation of Yersinia
Yersinia CIN agar is a selective isolation medium for the detection and differentiation of different
species of Yersinia from stool specimens.
The formula of the medium is that described by Schiemann (CIN medium: Cefsulodin, Irgasan,
Novobiocin).
The mannitol and neutral red present in the medium enable Yersinia to be differentiated
by the colour of the colonies (dark pink to red colonies).
The presence of cholate, deoxycholate, crystal violet, Irgasan and antibiotics inhibits the growth
of Gram-positive and most Gram-negative bacteria.
Incubation: 24 hours
Y. enterocolitica
ATCC 9610
Incubation: 48 hours
Y. enterocolitica ATCC 9610
Yersinia CIN agar
Ref.
Ref.
43 421 • kit of 20 plates
43 209 • kit of 100 plates
Clostridium difficile agar
2 Selective isolation of Clostridium difficile
Clostridium
difficile agar
Incubation: 48 hours
C. difficile ATCC 9689
Clostridium difficile agar is a selective isolation medium which is specially developed for the growth
of Clostridium difficile in stool specimens.
Clostridium difficile is a causative agent of Pseudo-membranous colitis and more generally
of antibiotic-associated diarrhea.
The presence of sheep blood facilitates the growth of the species being tested for.
The characteristic colonies are grey and sometimes spread along the inoculation streaks.
The antimicrobial and antifungal agents in the medium inhibit most bacterial and fungal
contaminants.
Clostridium difficile agar
Ref.
43 431 • kit of 20 plates
35 2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Pylori agar
Pylori agar
Incubation: 48 hours
H. pylori ATCC 43504
2 Selective isolation of Helicobacter pylori
H. pylori ATCC 43504
(x 2.5)
Pylori agar is a selective isolation medium for the detection of Helicobacter pylori in gastric biopsies.
Helicobacter pylori is a cause of gastritis and is associated with the development of gastric and
duodenal ulcers.
Helicobacter pylori is a microaerophilic bacterium whose viability is reduced on contact with air.
These characteristics should be taken into account for transport and culture.
The presence of horse plasma and PolyViteX enhances the growth of the target species.
The antibiotics present in the medium inhibit the majority of bacterial contaminants.
Pylori agar
Ref.
43 236 • kit of 10 plates
Portagerm pylori
Ref.
42 041 • kit of 8 tubes
Schaedler agar
+ 5% sheep
blood
Schaedler agar + 5% sheep blood
Incubation: 48 hours
B. fragilis ATCC 25285
Schaedler agar + 5 % sheep blood is an isolation medium particularly suitable for the detection
of obligate and facultative anaerobic bacteria.
The presence of growth factors such as yeast extract, hemin and vitamin K3 and the addition
of sheep blood, enable the growth of ever most fastidious species.
The reducing agent (L-cystine) and high concentration of dextrose in the agar favour the growth
of anaerobic species.
2 Isolation of anaerobic bacteria
Incubation: 72 hours
Veillonella parvula
ATCC 10790
Schaedler agar
+ 5 % sheep blood
Ref.
Ref.
Incubation: 24 hours
C. perfringens ATCC 13124
36
43 401 • kit of 20 plates
43 279 • kit of 100 plates
BCP agar
2 Isolation of common microorganisms
YBCP agar (Lactose agar with Bromcresol purple) is an isolation and differentiation medium
for the development of all organisms commonly found in specimens of various origin.
It also differentiates lactose-fermenting organisms from non-fermenting organisms.
Lactose (+) organisms produce yellow colonies by acidification of the medium.
Non-fermenting organisms produce blue or colourless colonies.
BCP agar
Incubation: 24 hours
E. coli ATCC 25922
(yellow colonies)
&
P. vulgaris ATCC 6380
(colourless colonies)
BCP agar
Ref.
43 021 • kit of 20 plates
Eosin-methylene blue agar
2 Isolation of Enterobacteriaceae
Eosin-methylene blue agar is a selective isolation and differentiation medium for the detection of
Enterobacteriaceae.
It enables lactose- and/or sucrose-fermenting microorganisms to be differentiated from nonfermenting microorganisms.
Eosin-methylene blue agar is used to detect enterobacteria in stools, urine or other biological
specimens.
Lactose (+) and/or sucrose (+) microorganisms produce dark purple colonies by acidification of the
medium, which may be accompanied by a metallic sheen.
Non-fermenting microorganisms produce colourless or slightly pinkish colonies.
The presence of 2 stains inhibits the growth of Gram-positive bacteria.
Eosinmethylene blue
agar
Incubation: 24 hours
E. coli ATCC 25922
Eosin-methylene blue agar
Ref.
43 081 • kit of 20 plates
37 2SIMPLE
M E D I A : I S O L AT I O N O F O R G A N I S M S
Mac Conkey
agar
Incubation: 24 hours
E. coli ATCC 25922
(pink colonies)
&
P. mirabilis
ATCC 12453
(colourless colonies)
Mac Conkey agar
2 Selective isolation of Enterobacteriaceae and Escherichia coli
MacConkey agar with crystal violet is a selective isolation and differentiation medium
for the detection of Enterobacteriaceae in specimens of various origins.
This medium is specially designed to detect the fermentation of lactose through a colour
change to neutral red.
• lactose (+) colonies: pink to red, sometimes surrounded by a halo of precipitated bile salts.
• lactose (-) colonies: colourless or slightly beige.
The selectivity for Gram-positive bacteria is provided by bile salts and crystal violet.
Storage: shelf-life includes 4 week storage at 15°-25°C.
MacConkey agar
Ref.
Ref.
Drigalski agar
Incubation: 24 hours
E. coli ATCC 25922
(yellow colonies)
&
Salmonella typhimurium
ATCC 14028
(blue colonies)
43 141 • kit of 20 plates
43 149 • kit of 100 plates
Drigalski agar
2 Selective isolation of Enterobacteriaceae
and other Gram-negative bacteria
Drigalski agar is a selective isolation and differentiation medium used to identify
Enterobacteriaceae and other Gram-negative bacteria in feces, urine or other biological specimens.
Microorganisms that ferment lactose form yellow or yellowish-green colonies;
the others produce blue, green, or bluish-green colonies.
The presence of sodium deoxycholate and crystal violet inhibits the growth of Gram-positive.
Drigalski agar
Ref.
38
43 341 • kit of 20 plates
SS agar
SS agar
Incubation: 24 hours
Salmonella typhimurium
ATCC 14028
(colonies
with black centre)
&
S. flexneri
ATCC 12022
(pale pink colonies)
2 Selective isolation of Salmonella and Shigella
SS agar is a selective isolation and differentiation medium recommended for the detection
of Salmonella and Shigella species.
The medium is inoculated from stool specimens, suspension of stools or an enrichment broth.
The medium detects lactose-fermenting and thiosulfate-reducing (production of H2S) colonies.
Lactose-fermenting microorganisms produce pink colonies, the others give colourless colonies.
H2S-producing microorganisms produce colonies with a black centre.
• Colonies of Salmonella are colourless to pale yellow, with or without a black centre.
• Colonies of Shigella are colourless to pale pink or orange-coloured without a black centre.
The inhibition of Gram-positive micro-organisms is obtained by a mixture of bile salts and stains.
SS agar
Ref.
Ref.
43 091 • kit of 20 plates
43 099 • kit of 100 plates
Rappaport broth
Ref.
42 091 • kit of 20 tubes
Selenite F broth
Ref.
42 099 • kit of 20 tubes
Hektoen agar
Hektoen agar
Incubation: 24 hours
Salmonella typhimurium
ATCC 14028
2 Selective isolation of Salmonella and Shigella
Hektoen agar is a selective isolation and differentiation medium recommended for the detection
of Salmonella and Shigella species.
The medium is inoculated from stool specimens, suspension of stools or an enrichment broth.
Microorganisms that ferment one of the three sugars in the medium form yellow or
yellowish-pink colonies; the others produce green to bluish-green colonies.
H2S-producing microorganisms form colonies with a black centre.
• Colonies of Salmonella are green to bluish-green, with or without a black centre.
• Colonies of Shigella are green to bluish-green without a black centre.
Inhibition of Gram-positive microorganisms is obtained by a mixture of bile salts and stains.
Hektoen agar
Ref.
Ref.
43 111 • kit of 20 plates
43 119 • kit of 100 plates
Rappaport broth
Ref.
42 091 • kit of 20 tubes
S. flexneri ATCC 12022
Selenite F broth
Ref.
42 099 • kit of 20 tubes
39 2SIMPLE
M E D I A : I S O L AT I O N O F O R G A N I S M S
CLED agar
Incubation: 24 hours
E.coli ATCC 25922
(yellow colonies)
&
P. mirabilis ATCC 12453
(colorless colonies)
CLED agar
2 Isolation of urinary tract micro-organisms
CLED agar (Cystine Lactose Electrolyte Deficient) is especially useful for the isolation of urinary tract
micro-organisms.
It also enables the differentiation of lactose-fermenting and non lactose-fermenting bacteria
(bromothymol blue indicator).
• Lactose (+) bacteria produce pale yellow to yellow colonies by acidification of the medium.
• Non-lactose fermenting bacteria produce green, blue or colourless colonies.
The composition of the medium (weak electrolyte content) prevents the swarming of Proteus.
The new CLED formula provides real advantages in terms of larger colony size, improvement
of Lactose character reading, and better colony differentiation.
CLED agar
Ref.
Ref.
Mannitol Salt 2
agar
Incubation: 24 hours
S. aureus ATCC 25923
43 331 • kit of 20 plates
43 339 • kit of 100 plates
CLED Andrade agar *
Ref.
43 072 • kit of 20 plates
Mannitol Salt 2 agar
2 Selective isolation of staphylococci
Mannitol Salt 2 agar is intended for the selective isolation of staphylococci in human specimens and
orientation of identification of S. aureus.
The new formula has increased nutrient capacity, sensitivity of detection and specificity.
Micro-organisms which ferment mannitol produce yellow colonies.
This characteristic is a criterion for orienting identification of Staphylococcus aureus.
The high content of sodium chloride in the medium limits the growth of certain bacteria other than
Staphylococcus.
Storage: shelf-life includes 4 week storage at 15°-25°C.
Mannitol Salt 2 agar
Ref.
Ref.
40
43 671 • kit of 20 plates
43 679 • kit of 100 plates
* Consult your local bioMérieux representative for further product information and availability.
2MEDIA
FOR SUSCEPTIBILITY TESTING
Mueller Hinton 2 agar
2 Susceptibility to antibiotics and sulfonamides
Mueller Hinton 2 agar is a medium for antimicrobial susceptibility testing by disc diffusion.
The composition of Mueller Hinton 2 agar enables the growth of non-fastidious bacteria
(Enterobacteriaceae, non fermenting Gram-negative bacilli, staphylococci and enterococci) found in
human pathology, while guaranteeing minimum interference from the constituents of the formula
with the result of the antimicrobial susceptibility test.
The concentration of divalent ions in the agar is adjusted to ensure a more accurate determination
of the susceptibility of Pseudomonas to aminoglycosides and tetracyclines.
The low concentration of thymine – thymidine (sulfonamide inhibitors) restricts the growth around
the discs, enabling more accurate measurement of the zones of inhibition.
The use of Mueller Hinton 2 agar is in compliance with the NCCLS and CA-SFM standards.
Mueller Hinton 2 agar
Ref.
Ref.
Mueller Hinton
2 agar
Incubation: 24 hours
E. coli ATCC 25922
Antibiotics
Ampicillin 10 µg
Cephalothin 30 µg
Gentamicin 10 µg
Nitrofurantoin 300 µg
Trimethoprim / sulfamethoxazole
1.25 / 23.75 µg
43 301 • kit of 20 plates
43 309 • kit of 100 plates
Mueller Hinton 2 agar
+ 5% sheep blood
2 Susceptibility of pneumococci and other streptococci to antibiotics and
sulfonamides
Mueller Hinton 2 agar + 5% sheep blood is a medium for antimicrobial susceptibility testing by disc
diffusion for strains requiring blood for their growth.
The composition of Mueller Hinton 2 agar, supplemented with sheep blood, enables the growth of
bacteria such as pneumococci and other streptococci, while guaranteeing minimum interference
from the constituents of the formula with the result of the antimicrobial susceptibility test.
The low concentration of thymine – thymidine (sulfonamide inhibitors) restricts the growth around
the discs, enabling more accurate measurement of the zones of inhibition.
The use of Mueller Hinton 2 agar is in compliance with the NCCLS and CA-SFM standards.
Mueller Hinton
2 agar + 5%
sheep blood
Incubation: 24 hours
S. pneumoniae ATCC 49619
Antibiotics
Oxacillin 1µg
Erythromycin 15 µg
Mueller Hinton 2 agar +
5% sheep blood
Ref.
Ref.
43 321 • kit of 20 plates
43 329 • kit of 100 plates
41 2MEDIA
F O R E N V I R O N M E N TA L C O N T R O L S : S U R FA C E S , A I R , W AT E R
Bi-BoxTM and
Count-Tact®
plates
surface
Count-Tact® range
The reference in environmental microorganism detection and/or enumeration
in hospitals
Count-Tact plates are 55mm in diameter and have a grid scored on the base.
The convex agar meniscus enables direct application to the test surface, such as walls,
floors, utensils, or employees, for hygiene controls.
Count-Tact plates are recommended to check critical control points in protected areas when
establishing or revising microbiological surface monitoring techniques and programs in
hospitals.
Count-Tact plates also can be used to control air, notably using an air sampler.
Count tact range plates 55mm: with neutralizers
For use in unprotected areas
®
Count-Tact
applicator
Count-Tact agar .......................................................................... Ref. 43 501 • kit of 20 plates
Count-Tact GTS agar without neutralizers ...................................... Ref. 43 582 • kit of 20 plates
Count-Tact Sabouraud Dextrose Chloramphenicol agar .................. Ref. 43 580 • kit of 20 plates
For use in clean areas triple-wrapped irradiated media 3P range: Storage at 2°-25°C
Count-Tact 3P Irradiated agar ...................................................... Ref. 43 691 • kit of 20 plates
................................................................................................ Ref. 43 699 • kit of 100 plates
Count-Tact Irradiated agar .......................................................... Ref. 43 491 • kit of 20 plates
................................................................................................ Ref. 43 492 • kit of 100 plates
Count-Tact Irradiated Sabouraud Dextrose Chloramphenicol agar .... Ref. 43 581 • kit of 20 plates
For use in isolators resistant to disinfection by disinfectant agents (Peroxyd or PerAcetic Acid)
Irradiated ISOLATOR Count - Tact 3P agar .................................... Ref. 43 597 • kit of 20 plates
Equipment (for plates 55mm)
42
The Count-Tact Applicator standardizes surface collections in terms of time and pressure
Count-Tact Applicator .................................................................. Ref. 96 300
The Bi-Box is a sterile box for collecting, transporting and incubating
Bi-BoxTM .......................................................... Ref. 96 301 • 10 sterile cases for Count-Tact plates
air IDEAL® is a aerobiocollector which guarantees effective measurement of aerobiocontamination
air IDEAL® 3PTM .......................................................................... Ref. 96 303
air & environment
90mm plates
For use in unprotected areas
Trypcase Soy agar ...................................................................... Ref. 43 011 • kit of 20 plates
................................................................................................ Ref. 43 019 • kit of 100 plates
Sabouraud Dextrose agar ............................................................ Ref. 43 555 • kit of 20 plates
Sabouraud Dextrose Chloramphenicol agar .................................. Ref. 43 596 • kit of 20 plates
Plates for
environmental
control
For use in clean areas triple-wrapped irradiated media 3P range: Storage at 2°-25°C
Trypcase Soy 3P Irradiated agar.................................................... Ref. 43 711 • kit of 20 plates
................................................................................................ Ref. 43 169 • kit of 100 plates
Trypcase Soy Irradiated agar ........................................................ Ref. 43 131 • kit of 20 plates
................................................................................................ Ref. 43 556 • kit of 100 plates
...................................................................................... Ref. 43 557 • kit of 20 plates (140mm)
Trypcase Soy Irradiated agar with neutralizers .............................. Ref. 43 562 • kit of 20 plates
Sabouraud Dextrose Irradiated agar ............................................ Ref. 43 554 • kit of 20 plates
Sabouraud Dextrose Chloramphenicol Irradiated agar .................. Ref. 43 595 • kit of 20 plates
Equipment (for plates 90mm)
The Bi-Box is a sterile box for collecting, transoporting and incubating
Bi-BoxTM 90 ................................................................................ Ref. 96 301 • 10 sterile cases
air IDEAL® is a aerobiocollector which guarantees effective measurement of aerobiocontamination
air IDEAL® 3PTM .......................................................................... Ref. 96 302
water
air IDEAL® 3PTM
Culture media and an identification test in compliance with the ISO 11731,
NF T 90-431 and NF T 90-461 standards.
Legionella
For detection and numeration
GVPC agar * .............................................................................. Ref. 43 031 • kit of 20 plates
................................................................................................ Ref. 43 032 • kit of 100 plates
For confirmation of the Legionella genus
BCYE with L-cysteine .................................................................. Ref. 43 013 • kit of 10 plates
BCYE without L-cysteine .............................................................. Ref. 43 023 • kit of 10 plates
For identification of L.pneumophila et L.anisa
SLIDEX® Legionella kit ** ............................................................ Ref. 73 120 • kit of 50 tests
Others
Enumeration of total viable aerobic microorganisms
R2A agar.................................................................................... Ref. 43 551 • kit of 20 plates
* Consult your local bioMérieux representative for further product information and availability.
** Not CE marked
43 Automated
specimen inoculation
44
A True Breakthrough
PREVI™ Isola is simply streaks ahead in automated
specimen inoculation.
chromIDTM
Candida
Automated inoculation
with mixed culture:
C. albicans,
C. tropicalis, C. krusei
It revolutionizes front-end media processing tasks by:
• Maximizing bacteria colony isolation
• Standardizing plate inoculation & results
• Streamlining your lab workflow
chromIDTM CPS®
Automated inoculation
E. coli ATCC 25922
Streaking Ahead
An original, revolutionary applicator using high precision
technology standardizes and maximizes colony inoculation:
• Maximum agar surface inoculated
• Optimum, pressure-controlled contact with the agar while
inoculating
• Standard quantity of inoculum used every time
This simple device is at the heart of PREVI™ Isola.
It will change the way you think about inoculation and
laboratory workflow.
45 The following eight "Specimen Protocols"
are intended to be used as guidelines for the main
types of specimen encountered in your laboratory.
They help you to position our culture media according
to specimen type with between I to V choices
possible. The choices indicated are the most
appropriate solution in terms of :
2 microorganisms tested
2 the examination performed
2 epidemiological data
2 cost / workflow ratio
46
Certain categories have been grouped together,
particularly as far as specimen collections are
concerned. For example, the "genital protocol" is for
both men and women. The analysis procedure
proposed should therefore be adapted to the
specimen to be studied as not all the microorganisms
indicated will necessarily be tested for.
Teaming our simple and practical protocols with our
extensive range of quality media provides you with
an optimised workflow solution.
Specimen Protocol Guidelines
“From Specimen to Plate”
48 2 Urine Protocol
49 2 Fecal Protocol
50 2 Genital Protocol
51 2 Suppuration Protocol
52 2 Throat Protocol
53 2 Sputum Protocol
54 2 Blood Culture Protocol
55 2 Cerebral Spinal Fluid Protocol
47 Color Gram 2
2 U R I N E P ROTOCOL
Slide/coverslide
SYSTEMATIC ISOLATION
E.coli/Proteus
NonEnterococcus
Staphylococcus
Enterobacteriaceae
Enterobacteriaceae
SPECIFIC ISOLATION
Gr.B
Streptococcus
chromIDTM
CPS®
I
UrilineTM
Uriline Coli
Uriline 3 Enterococcus
Yeasts
Streptococcus
Corynebact.
Gardnerella
Mycobacteriaceae
Columbia
CNA
Ziehl Kinyoun-Gabett
II
chromID
CPS
Columbia
CNA
III
CLED
IV
BCP
V
Mac
Conkey
48
OR
EMB
Löwenstein
Jensen
Columbia
CNA
or
TSA
OR Drigalski
Columbia
Coletsos
Color Gram 2
2 F EC AL P ROTOCOL
Methylene blue
Slide/coverslide
SYSTEMATIC ISOLATION
Salmonella
ENRICHMENT
SPECIFIC ISOLATION
Shigella
Yersinia
Campylobacter
E. coli
E. coli
O157H7
Clostridium
difficile
Vibrio
Hektoen
Yersinia
CIN
Campylosel
BCP
SMAC CT
C. difficile
chromIDTM
Vibrio
Rotavirus
Adenovirus
DIRECT
Rappaport
C
chromID
Salmonella
hCG 10
Hektoen
T
S
I
VIKIATM Rota-Adeno
Mac
Conkey
Selenite-F
II
OR
Drigalski
SS
OR
EMB
III
Mac
Conkey
49 Color Gram 2
2 G E N I TA L P R O T O C O L
PortagermTM
Giemsa
Slide/coverslide
SYSTEMATIC ISOLATION
Streptococcus /
Staphylococcus
Neisseria
I
Chocolate
PolyVitexTM
VCAT3
II
Chocolate
PolyVitex
III
Gonoline Duo 2
Columbia
CNA
chromIDTM
S.aureus
Columbia
CNA
Gardnerella
Enterobacteriaceae
Non-Enterobacteriaceae
Yeasts
Gardnerella
Mac
Conkey
chromID
Candida
Columbia
or
TSA
BCP
Sab Genta
Chloram 2
Mycoplasma
Chlamydiae
QuickVue®
Chlamydiae Test
Mycoplasma
IST 2
SPECIFIC ISOLATION
I
II
50
Anaerobes
Haemophilus
Listeria
Schaedler
Haemophilus
Chocolate 2
Columbia
CNA
Chocolate
PolyVitex
Columbia
or TSA
Group B Strepto
Pregnant woman
chromID
Todd Hewitt Strepto B
broth
Todd Hewitt
broth
Granada
Granada
biphasic
broth
Columbia
or TSA
2 S U P P U R AT I O N P R O T O C O L
PortagermTM
Amies agar
SYSTEMATIC ISOLATION
Staphylococcus
Streptococcus
Enterobacteriaceae
Non-Enterobacteriaceae
Color Gram 2
SPECIFIC ISOLATION
Anaerobes
Pasteurella
Yeasts
Corynebacteria
Chocolate
PolyVitexTM
chromID
Candida
Columbia
CNA
Schaedler
I
chromIDTM
S. aureus
Columbia
CNA
Mac
Conkey
Schaedler
Vit. K3
OR
Schaedler
Neo Vanco
Columbia
Sheep
II
Mannitol
Salt 2 agar
OR
Sab Genta
Chloram 2
Columbia
CNA
III
Columbia
CNA
51 2 T H R O AT P R O T O C O L
PortagermTM
Amies agar
SYSTEMATIC ISOLATION
I
Streptococcus
Staphylococcus
Neisseria /
Haemophilus
Columbia
CNA
chromIDTM
S. aureus
Chocolate
PolyVitexTM
SPECIFIC ISOLATION
Yeasts
Corynebacterium /
Arcanobacterium
Aspergillus niger
in ears
If necrotizing ulcerative
gingivitis (Vincent’s
tonsillitis suspicion)
chromID
Candida
Columbia
CNA
Sab Genta
Chloram 2
Schaedler
Sab Genta
Chloram 2
TSA
Haemophilus
Chocolate 2
II
III
52
TSA
Mannitol
Salt 2 agar
Chocolate
PolyVitex
VCAT3
Color Gram 2
Loeffler
Color Gram 2
2 S P UTU M P ROTOCOL
Giemsa
Ziehl (Kinyoun Gabett)
SYSTEMATIC ISOLATION
I
Streptococcus /
S. pneumoniae
Staphylococcus
Enterobacteriaceae
Non-Enterobacteriaceae
Haemophilus
Yeasts
Columbia
CNA
chromIDTM
S.aureus
Mac
Conkey
Haemophilus
Chocolate 2
chromIDTM
Candida
Chocolate
PolyVitexTM
VCAT3
Sab Genta
Chloram 2
Mannitol
Salt 2 agar
II
Mycobacteria
Löwenstein
Jensen
Coletsos
SPECIFIC ISOLATION
Aspergillus
Anaerobes
(if immunocompromised
patient)
Neisseria
Branhamella
Legionella
Burkholderia
cepacia
P. aeruginosa
RSV
Chocolate
PolyVitex
GVPC
BCSA
chromID
P. aeruginosa
RSV Direct IF
I
Schaedler
PortagermTM
Sab Genta
Chloram 2
OR
QuickVue®
RSV Test
53 2 B L O O D C U LT U R E P R O T O C O L
Blood culture
bottle
Color Gram 2
SYSTEMATIC ISOLATION
Enterobacteriaceae
NonEnterobacteriaceae
Streptococcus /
Enterococcus
Staphylococcus
Slidex® Pneumo-Kit
Columbia
or TSA
SPECIFIC ISOLATION
Yeasts
(If yeasts observed upon
direct examination)
III
chromIDTM
Candida
III
Sab Genta
Chloram 2
54
Brucella
TSA
Corynebacterium /
Listeria
Campylobacter
Neisseria /
Haemophilus
Anaerobes
Chocolate
PolyVitexTM
Schaedler
Color Gram 2
2 CEREBR AL SPINAL FLUID
Methylene blue
P ROTOCOL
Slide/coverslide
SYSTEMATIC ISOLATION
Neisseria /
Haemophilus
Streptococcus /
S. pneumoniae
Listeria
SPECIFIC ISOLATION
Staphylococcus
Enterobacteriaceae
NonEnterobacteriaceae
Mycobacteria
Ziehl Kinyoun-Gabett
Slidex® Meningite
Kit 5
I
Chocolate
PolyVitexTM
II
Chocolate
PolyVitex
VCAT3
Slidex Meningite
Strepto B
Yeasts
C. neoformans
(If yeasts observed upon
direct examination)
India Ink
Slidex Meningite
MnB / E. coli K1
Columbia
or TSA
Mac
Conkey
Löwenstein
Jensen
Coletsos
chromIDTM
Candida
Sab Genta
Chloram 2
55 Notes
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56
04-09 / 002GB99095B / This document is not legally binding. bioMérieux reserves the right to modify specifications without notice / BIOMERIEUX, the blue logo, API, air Ideal 3P, Bi-Box, BioBall, Bio-discs, chromID, Count-Tact, CPS, Hemoline, PolyVitex, Portagerm,
PREVI, Slidex, Uriline, Vikia are used, pending and/or registered trademarks belonging to bioMérieux S.A. or one of its subsidiaries / QuickVue is a trademark belonging to Quidel Corporation / ESwab is a trademark belonging to Copan / Any other name or trademark
is the property of its respective owner / bioMérieux SA RCS Lyon 673 620 399 / Photos: bioMérieux, N. Bouchut. Printed in France / THERA Conseil / RCS Lyon B 398 160 242.
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culture media
bioMérieux S.A.
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Tel. : 33 (0)4 78 87 20 00
Fax : 33 (0)4 78 87 20 90
www.biomerieux.com
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