GE Healthcare
Instructions 18-1028-73 AF
Superloop 150 ml
Introduction
Unpacking
TM
Superloop 150 ml is a large volume sample loop
recommended for sample volumes of 5–150 ml in the
flow rate range 0.5–100 ml/min. It can be loaded by either
a syringe or a pump. It can also be used as a reservoir
where a peak from a chromatographic run can be
collected and then injected onto another column.
Superloop 150 ml is a standard component that can be
TM
used with all ÄKTA design chromatography systems from
GE Healthcare. It is also available as a component for
other chromatography systems.
Superloop 150 ml is used together with an injection valve
such as MV-7 or INV-907 and replaces a simple sample
loop.
Specifications
Flow rate range:
Size:
Sample volume
range:
Pressure limit:
Minimum pressure
required for seal
movement:
Minimum pressure
required for opening
valve:
Wetted materials:
0.5–100 ml/min
340 x 45 mm
5–150 ml
2 MPa (20 bar, 300 psi)
When unpacking, please check the contents and carefully
examine the equipment for any visible signs of damage
that may have occurred during shipping.
Designation
No. per pack
Code No.
Superloop 150 ml
Instructions
1
1
18-1023-85
18-1028-73
Description
Superloop 150 ml consists of seven parts; two outer and
two inner end pieces, one glass tube, one movable seal
and a protective jacket (see cross-sectional diagram,
Fig 2).
The glass tube has a graduated scale of 5 ml increments
and seals against the inner end pieces. The protective
jacket is held in position by the outer end pieces.
A movable seal is situated in the glass tube between the
two inner end pieces. This seal divides the glass tube into
two separate chambers. Depending on the flow direction
the seal moves towards either end of the glass tube.
0.05 MPa (0.5 bar, 7.5 psi)
A springloaded valve made of titanium is located in the
center of the movable seal. When Superloop 150 ml is
in loading position, i.e. the movable seal is in the bottom
of the glass tube, the valve is closed and remains closed
during sample loading.
0.1 MPa (1 bar, 15 psi)
PTFE (polytetraflouroethylene),
EPDM (ethylenepropylendimonomer),
PEEK (polyetheretherketone),
borosilicate glass and titanium.
When the sample is injected onto the column the seal
moves downwards. When reaching the bottom position
the pressure increase causes the valve to open and the
buffer to pass through the valve (see Fig 1).
9. When the movable seal reaches the bottom end piece,
turn the valve to LOAD position.
Fig 1a. The movable seal moving down the glass tube.
Fig 2a. Valve position 1 – LOAD.
Fig 1b. When the end position is reached, the pressure increase
causes the valve to open.
Start up
Note: Before use, rinse the entire Superloop with
20% ethanol. Follow the ‘‘Disassembling’’ and
‘‘Reassembling’’ instructions in the Operations
section of theseinstructions.
Fig 2b. Valve position 2 – INJECT.
To column
From pump
1. Connect appropriate tubings to the inner end pieces.
2. Unscrew the upper outer end piece and take out the
inner end piece. If the inner end piece is difficult to
remove, the edge can be pried up carefully with a
spatula to get a sufficient grip.
7
1
2
6
3
5
4
Waste
Waste
3. Fill the upper chamber with eluent, i.e. start buffer for
gradient elution.
4. Insert the inner end piece and tighten it by screwing
the outer end piece into place. For the best operation,
make sure there is no trapped air.
5. Turn Superloop upside down and repeat 2 and 3 with
the bottom end piece.
6. Turn the V-7/IV-7/MV-7/IMV-7 to LOAD position and
connect Superloop to the valve as follows:
– Bottom end tubing to port 2.
– Upper end tubing to port 6.
7. Start the pump.
8. Turn the valve to INJECT position. The movable seal
now travels towards the bottom end piece and the
contents of the bottom chamber are injected onto the
column.
2
Instructions 18-1028-73 Edition AF
Fig 2c. Valve position 3 – WASH.
Operation
When the start-up procedure is completed Superloop is
ready to use.
1. Turn the injection valve to LOAD position. Load
Superloop with sample via valve port 3. The sample
can be transferred to the loop from a large volume
syringe or by using a pump. To connect a syringe use a
Luer-Lock fitting (Fig 3).
2. When Superloop is loaded and the column
equilibrated, turn the valve to INJECT position.
The sample will now be injected onto the column.
Depending on the experimental conditions, the loaded
sample can be injected all at once or in several smaller
volumes. For smaller volumes, turn the injection valve
to LOAD after the desired volume has left Superloop.
3
3. When the movable seal reaches the bottom end piece,
and all sample has been flushed out, turn the valve to
LOAD position. Superloop is now ready for reloading
(as described in section 1).
If the eluent is changed, Superloop must be rinsed out to
avoid undesired contamination. This is done by filling and
emptying the loop with the new eluent using a pump.
Fig 3.
CAUTION! The movable seal and the end pieces must
be inserted vertically. Make sure that all parts are well
supported to avoid tilting. If the tube is tilted during
mounting the angular force can cause breaking of the
tube.
Cleaning
Superloop is designed to facilitate Cleaning-In-Place (CIP).
This is achieved by pumping a cleaning or sanitizing agent
through Superloop. The standard recommendation is to
pump 0.5 M NaOH for 30 minutes.
Disassembling
The figure indications below refer to the cross-sectional
diagram in Figure 4.
1. Unscrew the outer upper end piece (1).
1
2
2. Pull out the protective jacket (5).
6
3. Take out the inner end piece (2).
If it is difficult to take out, the edge can be pried up
carefully with a spatula to get a sufficient grip.
5
4. Unscrew the outer bottom end piece (1).
5. Take out the inner end piece (2).
3
6. Push out the movable seal (4) carefully with a glass rod.
Reassembling
Before reassembling, wet the O-rings with distilled water
to lubricate them.
4
6
2
1. Insert the movable seal (4) into the glass tube from
above with the O-ring entering first.
2. Insert the inner bottom end piece (2).
3. Screw on the outer bottom end piece (1).
4. Push the protective jacket (5) into the end piece.
5. Insert the inner upper end piece (2).
1
1.
2.
3.
4.
5.
6.
Outer end piece
Inner end piece
Glass tube
Movable seal
Protective jacket
O-ring
Fig 4. Cross-sectional diagram.
6. Screw on the outer upper end piece (1).
Instructions 18-1028-73 Edition AF
3
Ordering information
Designation
Movable seal with flow
through valve
Inner end piece
O-ring inner end piece
Outer end piece
Glass tube
Union 1/16” male to
luer female
Union M6 male to
luer female
Union M6 female to
luer female
Trouble shooting
No.per pack
Code No.
1
1
2
1
1
18-1029-58
18-1029-59
18-1029-60
18-1118-61
18-1032-20
2
18-1112-51
2
18-1027-62
2
18-1027-12
www.gehealthcare.com
GE Healthcare Bio-Sciences AB
Björkgatan 30
751 84 Uppsala
Sweden
Problem
Possible cause
Action
Flow through
valve does
not function.
The movable
seal has been
inserted upside
down.
Trapped air in
the upper
chamber.
Disassemble
and reassemble.
Injected volume
is less than
expected.
Disassemble
and reassemble.
Superloop, BioPilot and Bio Process are trademarks of GE Healthcare
companies. GE, imagination at work and GE monogram are
trademarks of General Electric Company.
All goods and services are sold subject to the terms and conditions
of sale of the company within GE Healthcare which supplies them.
GE Healthcare reserves the right, subject to any regulatory and
contractual approval, if required, to make changes in specifications
and features shown herein, or discontinue the product described
at any time without notice or obligation. Contact your local GE
Healthcare representative for the most current information.
© 2006 General Electric Company – All rights reserved.
GE Healthcare Bio-Sciences AB, a General Electric Company.
GE Healthcare Bio-Sciences AB
Björkgatan 30
SE-751 84 Uppsala
Sweden
GE Healthcare Europe GmbH
Munzinger Strasse 5
D-79111 Freiburg
Germany
GE Healthcare UK Ltd
Amersham Place
Little Chalfont
Buckinghamshire, HP7 9NA
UK
GE Healthcare Bio-Sciences Corp
800 Centennial Avenue
P.O. Box 1327
Piscataway, NJ 08855-1327
USA
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Sanken Bldg. 3-25-1, Hyakunincho
Shinjuku-ku, Tokyo 169-0073
Japan
Elanders Östervåla 2006
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18-1028-73 AF 04/2006