Sample exam Senior
1.
In humans, implantation involves invasion finally reaching the:
a)
b)
c)
d)
2.
Uterine epithelium
Endometrial ECM (ExtraCellular Matrix)
Basement membrane of endometrial blood vessels
Lumen of endometrial blood vessels
This is a cross-section of a seminiferous tubule. Which cells do the arrows point to?
a)
b)
c)
d)
Spermatogonia
Elongated spermatids
Primary spermatocytes
Sertoli cells
3. Embryonic stem cells:
a)
b)
c)
d)
Are derived from trophectoderm
Cannot differentiate spontaneously to beating cardiac muscle cells
Can differentiate spontaneously to neural cells
Can be identified as pluripotent by the marker TGF alpha
4. According to the European Directive 2012/39/EU and as far as partner donation of
reproductive cells is concerned, the permitted time interval until repeat blood testing
is:
a)
b)
c)
d)
Up to 24 months
Every 6 months
Every time the samples are produced
Up to 12 months
5.
Which of the following statements is TRUE?
a)
b)
c)
d)
6.
All enzymes are proteins
All proteins are enzymes
Some RNAs are enzymes
Some DNAs are enzymes
The biggest challenge to cells during freezing is:
a) Their ability to endure storage at very low temperatures (< -180ºC)
b) Their ability to endure storage at very low temperatures (< -360ºC)
c) The lethality of an intermediate zone of temperature (-15ºC to -60ºC) that
they must traverse during cooling and again during warming
d) Tolerance to cryoprotectant agents
7.
Vitrified embryos:
a)
b)
c)
d)
8.
The following hormones are secreted by the anterior pituitary:
a)
b)
c)
d)
9.
Do not contain ice crystals
Are completely dehydrated
Can be safely stored at -80ºC
Can contain ice crystals although less than frozen embryos
Growth Hormone, Oxytocin, LH, ACTH
Prolactin, Oxytocin, TSH, FSH
TSH, ACTH, Prolactin, Growth Hormone
LH, ACTH, Antidiuretic Hormone, FSH
The process of karyokinesis:
a)
b)
c)
d)
Is dependent on the mitotic spindle
Is dependent on the action of actin and myosin filaments
Occurs only during the meiotic cleavage
Is regulated by protamines
10. Which statement about light exposure is incorrect?
a)
b)
c)
d)
Some spectra of light can generate reactive oxygen species
Light damage is related to exposure, wavelength but not intensity
Wavelengths <300nm ultraviolet can be absorbed by plastics
95% radiation is from microscopes
11. An individual with a balanced translocation:
a)
b)
c)
d)
Always shows evidence of a chromosomal abnormality
Invariably passes the translocation on to their child
May have a child without the translocation
Is unlikely to have fertility problems
12. The genes implicated in premature ovarian failure include:
a)
b)
c)
d)
SRY and SOX-9
AZF and ZFX
ZFX and FMR1
FMR1 and AMH
13. Which of the following growth factors has been implicated in early follicle recruitment
and growth?
a)
b)
c)
d)
GDF-9 (growth differentiation factor 9)
BMP-15 (bone morphogenetic protein 15)
Kit Ligand
All of the above
14. Regarding genomic imprinting, which of the following statements is true?
a) Large offspring syndrome in sheep is associated with disrupted IGF2R
imprinting
b) Erasure of imprints in the preimplantation embryo ensures correct
imprinting in each new generation
c) Methylation of imprinted genes is stable in cloned mice but not in cloned
sheep
d) PGD screening for IGF2R gene imprinting is now routine practice
15. One of the intended purposes of adding EDTA to the embryo culture media is:
a) To avoid ion chelation
b) To inhibit the premature stimulation of glycolysis of the cleavage stage
embryos
c) To favour the embryo development after compaction
d) All of the above are true
16. During female meiosis:
a)
b)
c)
d)
Meiosis I is completed during fetal development
Meiosis II commences during fetal growth
Meiosis I is arrested in diplotene during fetal development
Meiosis II is arrested in diplotene during fetal development
17. If a semen donor is positive for cytomegalovirus (CMV), then:
a) The donor semen must never be used in treatment
b) The semen may be used for general treatment if the donor is positive only for
IgM and not for IgG
c) The specimen should only be used for CMV-positive recipients
d) The specimen may be released for general treatment if the lab has standard
operating procedures (SOPs) governing the release of HCT/Ps (Human
cellular and tissue products) from CMV-positive patients
18. Grade A air quality, as defined by the Good Manufacturing Practice (GMP) guidelines,
is NOT required if:
a)
b)
c)
d)
Microbial inactivation or a validated terminal sterilisation process is applied
It is demonstrated that exposure to the conditions to obtain grade A air has a
detrimental effect on the tissue or cells
The mode of administration of the tissue or cells implies low or no risk to the
recipient
Answers a, b and c are correct