January, 2016
Volume 2, Number 2
Das Fliegen Ensemble and Maestro Helmut Shyster perform their winning entry in the Genetics Program Holiday Party
Song Contest 2015, "Odor to Joy" by Fruitflieg von Beethoven (Sung to the tune of Ode to Joy)
Drs. Chad Hunter and Nadia Singh
Drs. John Shorter and Trudy Mackay
Please join me in congratulating Drs. Chad Hunter
and John Shorter, our newest graduates of the
Genetics PhD program. Chad’s dissertation research
on “Environmental and Genetic Determinants of
Recombination Rate in Drosophila melanogaster”
was performed in the laboratory of Dr. Nadia Singh.
John’s dissertation research “Genetic Architecture of
Aggression in Drosophila melanogaster” was
performed in the laboratory of Dr. Trudy Mackay.
We have a busy upcoming Spring semester lined up,
with an excellent seminar series featuring alumni
speakers, a Distinguished Lecturer Series of studentinvited speakers, as well as internal and external
speakers. Please save February 19, 2016 for the 40th
annual Genetics Graduate Student Symposium and
recruitment weekend. The Symposium is entirely
organized and funded by the graduate students.
Come and support the graduate students, learn about
the exciting genetics research campus-wide, and meet
the applicants to the graduate program. Faculty
members, do not forget to let Melissa Robbins
([email protected]) know if you anticipate having
room in your lab for a graduate student in the Fall of
2016. We have some outstanding applicants!
I am pleased to announce that the Genetic Program
will be able to fund at least two Provost Research
Awards this semester. All Genetics graduate students
are eligible to apply for these awards of up to
$10,000. Application details are given below.
Trudy Mackay, Program Director
Calling All Graduate Students
Applications for Provost Research Awards
are due February 29, 2016. These $10,000
awards are for research supplies. It is
anticipated that there will at least two
awards. All graduate students are eligible.
Winners will be announced mid-March.
To apply, send a description of your research
project (maximum two pages) and a budget
(maximum one page) to Trudy Mackay
([email protected]) by the due date.
2016 Spring Seminars
All seminars are at 1:30 pm in the Stephens Room,
3503 Thomas Hall unless otherwise noted
January 11: Genetics Program Faculty meeting
January 18: No
Holiday
seminar, Martin Luther King
January25: Gustavo de los Campos, Department of
Epidemiology and Biostatistics, Michigan State
University
February 1: Jenny Tung, Department of Biology,
Duke University
February 8: Ed Buckler, USDA ARS and Plant
Breeding and Genetics Section, Cornell University
February 15: Ian Dworkin,
Biology, McMaster University
Department
of
NC State Alumnus Seminar
February 22: Jeremy Purvis, Department of Genetics,
UNC, Chapel Hill
February 29: Rasmus Nielsen, Department of
Integrative Biology, University of California, Berkeley
Genetics Program Distinguished Lecture Series,
Graduate Student Invited Speaker
March 7: No seminar, Spring Break
March 14: Johanna Elfenbein, Department
Clinical Sciences, NC State University
of
March 21: Nick Serão, Department of Animal
Science, NC State University
March 28: Jeff Sekelsky,
UNC, Chapel Hill
Biology
Department,
April 4: Brian Oliver, Laboratory of Cellular and
Developmental Biology, National Institute of
Diabetes and Digestive and Kidney Diseases
Genetics Program Distinguished Lecture Series,
Graduate Student Invited Speaker
April 11: Paul Garrity,
Brandeis University
Biology
Department,
Joint with the W. M. Keck Center for Behavioral
Biology
April 18: John Novembre, Department of Human
Genetics, University of Chicago
April 25: Aravinda Chakravarti, Institute of
Genetic Medicine, Johns Hopkins School of
Medicine
Genetics Program Distinguished Lecture Series,
Graduate Student Invited Speaker
May 2: Stephen Rich, Center for Public Health
Genomics, University of Virginia School of
Medicine
Spring 2016 Upcoming Events
February 18-20: Genetics
recruitment weekend
graduate
student
February 19: Genetics Graduate Student Annual
Symposium
Grants
Fikret Isik and co-investigators Jill Wegrzyn
(University of Connecticut), Andrew Eckert
(Virginia Commonwealth University) and Richard
Sniezko (USDA Forest Service, Dorena Genetic
Resource Center) received a $370,000 grant from
the USDA National Institute of Food and
Agriculture for their project “Towards Genomic
Breeding in Forest Trees”
David Reif and co-investigators from NC State
University (Fred Wright, Yihui Zhou) and from
Texas A&M University (Ivan Rusyn, David
Theadgill) received a $6 million grant from the
Environmental Protection Agency for their project
“Cardiotoxicity Adverse Outcome Pathway:
Organotypic Culture Model and in vitro-to-in vivo
Extrapolation for High-throughput Hazard, Doseresponse and Variability Assessments”
David Reif and co-investigator Robert Tanguay
(Oregon State University) received a $798,661 grant
from the Environmental Protection Agency for their
project “System Toxicological Approaches to Define
Flame Retardant Adverse Outcome Pathways”
Out and About
Jose Alonso was an invited speaker and also gave a
poster presentation at the Annual Plant Genome
Awardee Meeting in Arlington, VA
Jose Alonso attended and was an invited speaker at
the Workshop on Plant Development and Drought
Stress at the Asilomar Conference Grounds, Pacific
Grove, CA
Jose Alonso attended the 29th Annual Plant
Molecular Biology Retreat in Asheville, NC
Robert Anholt was an invited speaker at the EMBO
Meeting in Birmingham, England
Robert Anholt was an invited speaker at the
European Drosophila Research Conference in
Heidelberg, Germany
Jose Brumos and colleagues Anna Stepanova, Jose
Alonso C. Merchante, Q Hu and S. Heber gave an
oral presentation on “Regulation of protein synthesis
in ethylene signaling” at the 29th Annual Plant
Molecular Biology Retreat in Asheville, NC
Jose Brumos presented a seminar on his work and
that of his colleagues Anna Stepanova and Jose
Alonso (“New Insights into Ethylene Signaling and
Auxin Biosynthesis”) to the Department of Plant and
Microbial Biology, NC State University
Trudy Mackay was an invited speaker at the EMBO
Meeting in Birmingham, England
Trudy Mackay gave a plenary lecture at the European
Drosophila Research Conference in Heidelberg,
Germany
Trudy Mackay presented the John A. Lynch Lecture
of the College of Science of the University of Notre
Dame, Notre Dame IN
Trudy Mackay gave a seminar to the Department of
Biochemistry and Genetics, Clemson University,
Clemson SC
Anna Stepanova presented a talk on “Metabolism
and coordinated cellular proliferation: lessons from
low-cell-density mutants” at the Workshop on Plant
Development and Drought Stress at the Asilomar
Conference Grounds, Pacific Grove, CA
Of Note…
The Alonso-Stepanova lab gave two hour
Plants4Kids outreach demos at the NC Museum of
Natural Sciences, Science Saturday on September 5,
October 3, November 7, December 5 2015 and
January 2, 2016.
Jennifer Baltzegar, along with Sophia Webster (Max
Scott Lab) and Johanna Elsensohn (Hannah Burrack
Lab), won the Best Overall Team in the
Entomological Society of America Student Debates
at the ESA Conference in Minneapolis, MN in
November. Jenn’s team was randomly assigned to
debate the con side of the topic: "With the
development of tools like RNAi, in the future we
may be capable of eradicating species. If we can
eradicate a species, should we?"
Trudy Mackay chaired the November 2015
Scientific Advisory Board meeting of the Max Planck
Institute for Developmental Biology, Tübingen
Germany
Trudy Mackay served on the 2015 Royal Society
Newton Advanced Fellowship Committee
Trudy Mackay served on the search committee for
the Director of the National Library of Medicine
David Reif served as a member of an expert
committee that authored the report "Application of
Modern Toxicology Approaches for Predicting
Acute Toxicity for Chemical Defense" for the
National Academies of Sciences, Engineering, and
Medicine.
David Reif was a member of the Working Group for
the World Health Organization's (WHO)
International Agency for Cancer Research (IARC)
which met to evaluate the carcinogenicity of the
insecticides gamma-hexachlorocyclohexane (lindane)
and dichlorodiphenyltrichloroethane (DDT) and the
herbicide 2,4-dichlorophenoxyacetic acid (2,4-D)
New Approaches in Studying Recent
Polygenic Adaptation in Humans
by
Sam Widmayer
On October 12, 2015,
the Genetics Graduate
Students hosted Dr.
Jonathan Prichard, a
Professor
in
the
Departments of Biology
and Genetics at Stanford University, as well as a
Howard Hughes Medical Institute Investigator. Dr.
Pritchard is well known for his contributions to
population genetics and genomics, touting an
impressive 15,598 citations to date for his signature
“STRUCTURE” method. The work in his
laboratory is currently focused in human population
genetics, leveraging large genome-wide data sets to
detect signatures of population structure, gene flow,
and selection in both short-term and long-term
human history.
In the first part of Dr. Pritchard’s talk, he delved into
how his lab uses genetic information to learn about
population structure and historic relatedness among
populations. His lab has developed a method that
uses allele frequency covariance matrices to support
traditional bifurcating tree models of evolution, while
taking into account potential gene flow between
lineages. This makes models estimating relatedness
among populations more robust to gene flow. Dr.
Pritchard used systems to illustrate this concept:
diverse dog breeds, and human populations. Despite
being less genetically related through long-term
evolutionary history, many dog breeds resemble each
other phenotypically. Using this model taking into
account gene flow between populations, Dr.
Pritchard showed that phenotypically similar dog
breeds share histories of strong selection for genes
influencing these traits, such as dogs of short stature
and the IGF1 locus. Contrasted with that of certain
dog breeds, the role of selection in human evolution
appears to be much less clear. Loci influencing skin
pigmentation, hair morphology, and sweat glands all
exhibit weak signs of selection in this model, but
other loci detected are either poorly characterized or
play less obvious roles in human adaptation. These
vignettes by Dr. Pritchard exemplified a unique twist
on classical models of adaptation by taking into
account historic gene flow, which may have
substantial implications for understanding human
evolution.
In the latter half of the talk, Dr. Pritchard illustrated
multiple ways his lab is looking for signatures of
both recent and ancient selection in human
populations. He began by contrasting two genetic
models of selection, the hard sweep model and the
polygenic adaptation model, emphasizing the power
of the latter model to connect evolutionary histories
and complex quantitative traits. Northern Europeans
are taller on average than their southern European
neighbors, serving as a starting point to understand
the basis of polygenic adaptation. By polarizing
SNPs in northern and southern Europeans by their
effect on height and comparing their allele
frequencies in each population, Dr. Pritchard found
that alleles that increase height tended to be at high
frequency in northern Europeans. Since this method
relies on allele frequency differences between
populations, Dr. Pritchard was interesting in
developing an alternative method to detect alleles
under selection. Singleton density scores (SDS)
leverage the tendency for alleles under selection to
accelerate branching rates in trees, which reduces the
number of associated singleton alleles compared to
alleles that aren’t under selection. Using this method,
Dr. Pritchard was able to mine genome-wide data
from British populations and find loci under recent
selection, such as those associated with blue eye color
and lactose intolerance. Further, by analyzing similar
data from many more GWAS, Dr. Pritchard detected
signatures of ancient selection for a number of
complex traits, including increasing head
circumference, increased hip width, increased birth
weight, decreased diabetes risk, and increased
ulcerative colitis risk.
Dr. Pritchard left the Program in Genetics with a few
considerations. By looking at large genomic data sets
and accounting for migration and selection, Dr.
Pritchard illustrated how it is possible to make
conclusions about the path of human evolution on
multiple time scales. Further, this path will likely
reflect substantial impacts from migration between
populations in the past, and selection acting in recent
history. Lastly, Dr. Pritchard emphasized analyses
like these will put the genetic dissection of complex
traits in a new context for understanding human
evolution and population genomics.
Pituitary Gland Development and Disease:
Stem Cells and Genetic Modifiers
by
Andrea Vogel
Dr. Sally Camper, James V. Neel
Professor and Chair of the
Department of Human Genetics at
the University of Michigan, visted
on November 2nd as a Genetics
Graduate Student invited speaker.
She met with many professors during the day, and
had lunch with the graduate students of the Genetics
Program. Lunch was interesting, as Dr. Camper
regaled the students with her experiences of
becoming a professor, and talked about how being a
TA, when she was a graduate student, helped her
determine her future career path. Dr. Camper’s
seminar was well attended, and described genes
responsible for the development and regulation of the
pituitary gland. She talked in-depth about the gene
Prop1, which, when it has transcription factor
mutations, is the most common cause for multiple
pituitary hormone deficiencies. She also talked about
her dwarf line of mice, which is used to determine the
genetic basis of hearing impairment. The type of
hearing impairment Dr. Camper studies is associated
with thyroid dysfunction, and also is related to
Prop1. It was an intriguing talk, and the graduate
students really appreciated Dr. Camper’s visit.
Of Gars, Zebrafish, and Men
by
Emily Moore
On Monday, November 16th, Dr.
John Postlethwait, a Professor in
the Department of Biology at the
University of Oregon, visited us as
a Genetics Graduate Student
invited speaker. His riveting talk,
“Linking Human Biology to Fish
Models: The Gar Bridge,” was well received by
NCSU faculty and students alike.
Teleost fishes are a species-rich and diverse group,
and include taxa that make excellent models of
vertebrate development, human disease, and
toxicological response. Dr. John Postlethwait is well
known for his work developing zebrafish (Danio
rerio) as a developmental genetic model, but also
works with many other teleost species, including
Antarctic icefishes to investigate bone development,
blind cave fish (Sinocyclocheilus spp.) to investigate
eye development, and stickleback (Gasterosteus spp.)
to investigate the effect of perchlorate toxicity on
gonad development. Despite the many useful features
of teleost fish (ease of keeping, wide array of
phenotypes, conserved vertebrate developmental
mechanisms), a whole-genome duplication that
occurred in the teleost lineage complicates the
identification of orthologous genes in mammals after
they have been identified in a teleost fish model.
Following whole-genome duplication, the ohnologs
(homologous genes that are related through genome
duplication) can have three possible fates: nonfunctionalization, neo-functionalization, and subfunctionalization. Postlethwait estimates that 7580% of all ohnologs become non-functional or are
lost, though there is still an open question of how
many genes evolve new functionality or experience
reciprocal loss of function (where each copy retains a
subset of the original functionality). Differential gene
loss after genome duplication can lead to the
erroneous conclusion that ohnologs are orthologs,
which can confound understanding of conserved
function and evolutionary distance. When the teleost
genomes are compared to the human genome, more
than 870 genes are missing in the fishes, but without
the right comparator, it is impossible to determine
whether these are novelties in the tetrapods, or losses
in the teleosts.
Fortunately, Dr. Postlethwait has developed a
resource to connect teleost sequences to those of
human and mouse—the spotted gar (Lepisosteus
oculatus) genome. Gars are basal bony fish that
diverged before the teleost whole-genome
duplication, and can be used as an intermediary to
help the genetics community relate findings in
zebrafish to human, and vice-versa. He calls this the
“Gar Bridge,” where comparison to the relatively
slowly evolving gar sequence can highlight both
coding and regulatory conservation across vertebrates.
The gar genome has surprisingly high shared synteny
with chicken chromosomes, despite the 450 million
year distance, with over 50% of the genome
corresponding in a 1:1 manner. In contrast, for gar
and the teleost fish medaka (Orizias latipes), each gar
chromosome maps to two medaka chromosomes,
indicating chromosome fusion events occurred before
the teleost whole-genome duplication and subsequent
rediploidization. To demonstrate the power and
utility of the “Gar Bridge,” Dr. Postlethwait showed
that a conserved non-coding element previously
thought to be specific to tetrapod limb development
can be found in zebrafish, if you align the sequence
to the gar genome rather than to the mouse genome.
With this new tool in our toolbox, it is now easier
than ever to use the genetic findings from fish models
to help us assign function to both coding regions of
genes and regulatory elements found in GWAS of
human related diseases.
Genetic Basis for Behavioral
Isolation Leading to Speciation
By
Jennifer Baltzegar
On November 30, 2015 the
Genetics Program was proud to
host Dr. Amanda Moehring,
Associate Professor in the
Department of Biology at Western
University in Ontario, Canada, as
the featured Alumna Speaker of the
semester. Dr. Moehring completed her doctoral work
in the Mackay Lab. Her current research focuses on
the genetic basis of behavioral differences and
speciation. Her talk was entitled “Genetic, Neural,
Behavioral: The Basis of Female Mate Preference and
Species Isolation.”
Dr. Moehring began her talk by emphasizing the
concept of speciation. On a tree of life, each branch
represents a unique species that does not exchange
genes with other branches. Using this representation,
speciation is when new species form and biodiversity
is maintained. She is interested in mechanisms of
species isolation. The major ones being hybrid
dysfunction and behavioral traits, which result in
restricted gene flow. Male traits and female
receptivity contribute to behavioral isolation, with
female receptivity acting as the final barrier to species
isolation. Moehring’s major research focus is on the
genetic basis of behavioral isolation.
Prior to this study there were no known genes that
govern behavioral isolation. Moehring utilized
multiple Drosophila species for this work because of
the wealth of genetic tools available for the model as
well as the reliability for creating hybrid species in
the lab. One advantageous characteristic of
Drosophila crosses is the phenomenon of choosiness.
For example, female D. melanogaster can be crossed
easily to male D. simulans in the lab, but the cross
between female D. simulans and male D.
melanogaster is much more difficult. The female D.
simulans are said to be “choosy.”
Moehring conducted deficiency mapping using
hemizygous individuals to identify genes that control
this “choosy” behavior on several different species
crosses. She was able to identify a region of interest
on chromosome 3R that contained only ten genes.
She then used single gene disruption to characterize
the effect of each candidate gene. In the end, three
genes were identified that were associated with female
receptivity: Ntu, Nmt, and Ntu.
The gene named Ntu, “It’s not you, it’s me”, which
encodes a microtubule binding protein associated
with axon patterning, was identified as having a
significant effect on behavior. In addition to the
work on Ntu, the other two genes identified were
also implicated in female preference: Ntd, “Not
tonight dear” and Nmt, “Not my type.” Ntd encodes
a DNA-binding transcription factor that has also
been associated with axon patterning and Nmt
encodes a protein kinase that has been implicated in
stress response.
Since Ntu (as well as Ntd) was associated with axon
patterning, Moehring followed up the gene
identification with neurobiological work to
determine if there is a neural basis for behavioral
differences that maintain species. Her team found
that the mushroom bodies of the female Drosophila
brain are associated with this gene in this process.
In summary, Moehring and her research group have
identified three genes associated with behavioral
isolation in Drosophila: Ntu, Nmt, and Ntd. In
addition, they have shown that the mushroom bodies
in Drosophila brains may be contributing to this
behavioral isolation due to female receptivity.
Publications
The following publications of members of the
Program in Genetics have appeared in print since the
last issue of the ORF. Names in bold font are current
members of the Program in Genetics
Anderson CM, Langerhans RB. 2015. Origins of
female genital diversity: Predation risk and lock-andkey explain rapid divergence during an adaptive
radiation. Evolution 69(9): 2452-67.
Bian Y, Holland JB. 2015. Ensemble learning of
QTL models improves prediction of complex traits.
G3 (Bethesda) 5(10): 2073-84.
Carnes MU, Campbell T, Huang W, Butler DG,
Carbone MA, Duncan LH, Harbajan SV, King EM,
Peterson KR, Weitzel A, Zhou S, Mackay TFC.
2015. The genomic basis of postponed senescence in
Drosophila melanogaster. PLoS One 10(9):
e0138569.
Dumont BL, Devlin AA, Truempy DM, Miller JC,
Singh ND. 2015. No evidence that infection alters
global recombination rate in house mice. PLoS One
10(11): e0142266.
Elvers I, Turner-Maier J, Swofford R, Koltookian M,
Johnson J, Stewart C, Zhang CZ, Schumacher SE,
Beroukhim R, Rosenberg M, Thomas R, Mauceli E,
Getz G, Palma FD, Modiano JF, Breen M, LindbladToh K, Alföldi J. 2015. Exome sequencing of
lymphomas from three dog breeds reveals somatic
mutation patterns reflecting genetic background.
Genome Res 25(11): 1634-45.
Garlapow ME, Huang W, Yarboro MT, Peterson
KR, Mackay TFC. 2015. Quantitative genetics of
food intake in Drosophila melanogaster. PLoS One
10(9): e0138129.
Gralinski LE, Ferris MT, Aylor DL, Whitmore AC,
Green R, Frieman MB, Deming D, Menachery VD,
Miller DR, Buus RJ, Bell TA, Churchill GA,
Threadgill DW, Katze MG, McMillan L, Valdar W,
Heise MT, Pardo-Manuel de Villena F, Baric RS.
2015. Genome wide identification of SARS-CoV
susceptibility loci using the collaborative cross. PLoS
Genet 11(10): e1005504.
Hecht ES, Scholl EH, Walker SH, Taylor AD, Cliby
WA, Motsinger-Reif AA, Muddiman DC. Relative
quantification and higher-order modeling of the
plasma glycan cancer burden ratio in ovarian cancer
case-control samples. J Proteome Res 14(10): 4394401.
Hensley MT, de Andrade J, Keene B, Meurs K, Tang
J, Wang Z, Caranasos TG, Piedrahita J, Li TS,
Cheng K. 2015. Cardiac regenerative potential of
cardiosphere-derived cells from adult dog hearts. J
Cell Mol Med 19(8): 1805-13.
Holland JB. 2015. MAGIC maize: a new resource for
plant genetics. Genome Biol 6: 163.
Horn BW, Gell RM, Singh R, Sorensen RB,
Carbone I. 2016. Sexual reproduction in Aspergillus
flavus Sclerotia: Acquisition of novel alleles from soil
populations and uniparental mitochondrial
inheritance. PLoS ONE 11(1): e0146169.
Howard JT, Haile-Mariam M, Pryce JE, Maltecca C.
2015. Investigation of regions impacting inbreeding
depression and their association with the additive
genetic effect for United States and Australia Jersey
dairy cattle. BMC Genomics 16(1): 813.
Howard JT, O'Nan AT, Maltecca C, Baynes RE,
Ashwell MS. 2015. Differential gene expression
across breed and sex in commercial pigs administered
fenbendazole and flunixin meglumine. PLoS One
10(9): e0137830.
Hu YJ, Sun W, Tzeng JY, Perou CM. 2015. Proper
use of allele-specific expression improves statistical
power for cis-eQTL mapping with RNA-seq data. J
Am Stat Assoc 110(511): 962-974.
Huang W, Carbone MA, Magwire MM, Peiffer JA,
Lyman RF, Stone EA, Anholt RR, Mackay TFC.
2015. Genetic basis of transcriptome diversity in
Drosophila melanogaster. Proc Natl Acad Sci U S A
112(44): E6010-9.
Isik F, Bartholomé J, Farjat A, Chancerel E, Raffin A,
Sanchez L, Plomion C, Bouffier L. 2016. Genomic
selection in maritime pine. Plant Sci 242: 108-19.
Jack J, Havener TM, McLeod HL, Motsinger-Reif
AA, Foster M. 2015. Evaluating the role of
admixture in cancer therapy via in vitro drug response
and multivariate genome-wide associations.
Pharmacogenomics 16(13): 1451-63.
Jamann TM, Balint-Kurti PJ, Holland JB. 2015.
QTL mapping using high-throughput sequencing.
Methods Mol Biol 1284: 257-85.
Lassiter ES, Russ C, Nusbaum C, Zeng Q, Saville
AC, Olarte RA, Carbone I, Hu CH, Seguin-Orlando
A, Samaniego JA, Thorne JL, Ristaino JB. 2015.
Mitochondrial genome sequences reveal evolutionary
relationships of the Phytophthora 1c clade species.
Curr Genet 61(4): 567-77.
Linger RJ, Belikoff EJ, Scott MJ. 2015. Dosage
compensation of X-linked Muller element F genes
but not X-linked transgenes in the Australian sheep
blowfly. PLoS One 10(10): e0141544.
Liu H, Lamm MS, Rutherford K, Black MA,
Godwin JR, Gemmell NJ. 2015. Large-scale
transcriptome sequencing reveals novel expression
patterns for key sex-related genes in a sex-changing
fish. Biol Sex Differ 6: 26.
Loomis D, Guyton K, Grosse Y, El Ghissasi F,
Bouvard V, Benbrahim-Tallaa L, Guha N, Mattock
H, Straif K; International Agency for Research on
Cancer Monograph Working Group (incl. Reif
DM), IARC, Lyon, France. 2015. Carcinogenicity of
lindane, DDT, and 2,4-dichlorophenoxyacetic acid.
Lancet Oncol 16(8): 891-2.
Loziuk PL, Parker J, Li W, Lin CY, Wang JP, Li Q,
Sederoff RR, Chiang VL, Muddiman DC. 2015.
Elucidation of xylem-specific transcription factors
and absolute quantification of enzymes regulating
cellulose biosynthesis in Populus trichocarpa. J
Proteome Res 14(10): 4158-68.
Marayati BF, Schal C, Ponnusamy L, Apperson CS,
Rowland TE, Wasserberg G. 2015. Attraction and
oviposition preferences of Phlebotomus papatasi
(Diptera: Psychodidae), vector of Old-World
cutaneous leishmaniasis, to larval rearing media.
Parasit Vectors 8(1): 663.
Martins TS, Sanglard LM, Silva W, Chizzotti ML,
Rennó LN, Serão NV, Silva FF, Guimarães SE,
Ladeira MM, Dodson MV, Du M, Duarte MS.
2015. Molecular factors underlying the deposition of
intramuscular fat and collagen in skeletal muscle of
Nellore and Angus cattle. PLoS One 10(10):
e0139943.
Meisner M, Reif DM. 2015. Computational
methods used in systems biology. pp 85-115 in
Systems Biology in Toxicology and Environmental
Health. Elsevier. ISBN 9780128015643.
Merchante C, Brumos J, Yun J, Hu Q, Spencer KR,
Enríquez P, Binder BM, Heber S, Stepanova AN,
Alonso JM. 2015. Gene-specific translation
regulation mediated by the hormone-signaling
molecule EIN2. Cell 163(3): 684-97.
Mochizuki H, Shapiro SG, Breen M. 2015.
Detection of BRAF mutation in urine DNA as a
molecular diagnostic for canine urothelial and
prostatic carcinoma. PLoS One 10(12): e0144170.
Moreno-Risueno MA, Sozzani R, Yardımcı GG,
Petricka JJ, Vernoux T, Blilou I, Alonso J, Winter
CM, Ohler U, Scheres B, Benfey PN. 2015.
Transcriptional control of tissue formation
throughout root development. Science 350: 426-30.
Morozova TV, Huang W, Pray VA, Whitham T,
Anholt RR, Mackay TFC. 2015. Polymorphisms in
early neurodevelopmental genes affect natural
variation in alcohol sensitivity in adult Drosophila.
BMC Genomics 16(1): 865.
Niederwerder MC, Bawa B, Serão NV, Trible BR,
Kerrigan MA, Lunney JK, Dekkers JC, Rowland RR.
2015. Vaccination with a porcine reproductive and
respiratory syndrome (PRRS) modified live virus
vaccine followed by challenge with PRRS virus and
porcine circovirus Type 2 (PCV2) protects against
PRRS but enhances PCV2 replication and
pathogenesis compared to results for nonvaccinated
cochallenged controls. Clin Vaccine Immunol
22(12): 1244-54.
Odet F, Pan W, Bell TA, Goodson SG, Stevans AM,
Yun Z, Aylor DL, Kao CY, McMillan L, de Villena
FP, O'Brien DA. 2015. The founder strains of the
collaborative cross express a complex combination of
advantageous and deleterious traits for male
reproduction. G3 (Bethesda) 5(12): 2671-83.
Okagaki LH, Nunes CC, Sailsbery J, Clay B, Brown
D, John T, Oh Y, Young N, Fitzgerald M, Haas BJ,
Zeng Q, Young S, Adiconis X, Fan L, Levin JZ,
Mitchell TK, Okubara PA, Farman ML, Kohn LM,
Birren B, Ma LJ, Dean RA. 2015. Genome sequences
of three phytopathogenic species of the
magnaporthaceae family of fungi. G3 (Bethesda)
5(12): 2539-45.
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