Kansliets noteringar
Kod
Dnr
2007-8680
2007-14950-54750-89
2007
*Linnéstöd och Berzelius Center
Area of science
Vetenskapsrådet
Announced grants
Linnaeus Grant 2007
Total amount for which applied (kSEK)
2008
2009
2010
2011
2012
2013
2014
2015
2016
2017
7500
7500
7500
7500
7500
7500
7500
7500
7500
7500
2018
REPRESENTATIVE FOR THE HEI
Name(Surname, First name)
Markides, Karin
Date of birth
511124-1021
Sex
Female
Email address
[email protected]
Academic title
Professor
Position
President
Phone
031 772 25 50
ORGANISATION
Organisation
Chalmers University of Technology
DESCRIPTIVE DATA
Project title, English (max 200 char)
Centre for Bio-inspired Supramolecular Function and Design
Abstract (max 1500 char)
The project will focus on bio-inspired supramolecular systems for studying nanoscale chemical and physical processes of
biophysical and technological relevance. We will develop and study, from fundamental, methodological as well as applied angles,
systems based on nucleic acids or hosted by lipid membranes, exploiting electronic properties and intermolecular recognition
mechanisms, to achieve controlled non-periodic structures with designed functionalities. Optical spectroscopic methods are
developed to gain detailed information about structure, dynamics and electronic properties of the systems and their constituents, but
also as basis for development of new supra-molecular functionalities. Unique competencies at the Chemistry and Physics
Departments will be twinned in PhD projects combining the participants’ current research interests. The Centre will define a
long-term strategic platform of generic tools for addressing applied problems: from micro-reactor and enzyme technology, via
medicinal and sensor applications, to bottom-up molecular nano-technology, nano-optics and molecular electronics. One goal of
the supra-molecular effort is to bridge the gap between “chemical” (molecules) and “physical” (lithography) dimensions of
nano-science. As a result of close collaboration and integration of widely different competencies among us we anticipate true
synergism and new insights to be reached of great fundamental as well as applied impact.
Abstract language
Vetenskapsrådet, SE-103 78 Stockholm, tel. +46 (0)8 546 44 000, [email protected]
Kod
2007-14950-54750-89
Name of Applicant
Markides, Karin
Date of birth
511124-1021
English
Research areas
*Naturvetenskap
Review panel
VR-N
Classification codes (SCB) in order of priority
151201, 150413, 151205
OTHER CO-WORKING RESEARCHERS
Name(Surname, First name)
University/corresponding, Department, Section/Unit, Addressetc.
Nordén, Bengt
Chalmers University of Technology
Chemical and Biological Engineering
Date of birth
Sex
450515
Male
Academic title
Date of doctoral exam
Professor
1971-05-25
Name(Surname, First name)
University/corresponding, Department, Section/Unit, Addressetc.
Albinsson, Bo
Chalmers University of Technology
Chemical and Biological Engineering
Date of birth
Sex
630201
Male
Academic title
Date of doctoral exam
Professor
1993-03-15
Name(Surname, First name)
University/corresponding, Department, Section/Unit, Addressetc.
Andersson, Mats
Chalmers University of Technology
Chemical and Biological Engineering
Date of birth
Sex
660527
Male
Academic title
Date of doctoral exam
Professor
1995-03-24
Name(Surname, First name)
University/corresponding, Department, Section/Unit, Addressetc.
Gunnarsson, Linda
Chalmers University of Technology
Applied Physics
Date of birth
Sex
711229
Female
Academic title
Date of doctoral exam
PhD
2004-01-20
Name(Surname, First name)
University/corresponding, Department, Section/Unit, Addressetc.
Holmberg, Krister
Chalmer University of Technology
Chemical and Biological Engineering
Date of birth
Sex
460316
Male
Academic title
Date of doctoral exam
Professor
1974-05-30
Name(Surname, First name)
University/corresponding, Department, Section/Unit, Addressetc.
Höök, Fredrik
Chalmers University of Technology
Applied Physics
Date of birth
Sex
661114
Male
Academic title
Date of doctoral exam
Professor
1997-12-16
Vetenskapsrådet, SE-103 78 Stockholm, tel. +46 (0)8 546 44 000, [email protected]
Kod
2007-14950-54750-89
Name of Applicant
Markides, Karin
Date of birth
511124-1021
Name(Surname, First name)
University/corresponding, Department, Section/Unit, Addressetc.
Käll, Mikael
Chalmers University of Technology
Applied Physics
Date of birth
Sex
630509
Male
Academic title
Date of doctoral exam
Professor
1995-06-22
Name(Surname, First name)
University/corresponding, Department, Section/Unit, Addressetc.
Olsson, Lisbeth
Chalmers University of Technology
Chemical and Biological Engineering
Date of birth
Sex
631122
Female
Academic title
Date of doctoral exam
Professor
1994-05-22
Name(Surname, First name)
University/corresponding, Department, Section/Unit, Addressetc.
Orwar, Owe
Chalmers University of Technology
Chemical and Biological Engineering
Date of birth
Sex
640111
Male
Academic title
Date of doctoral exam
Professor
1994-05-20
Name(Surname, First name)
University/corresponding, Department, Section/Unit, Addressetc.
Åkerman, Björn
Chalmers University of Technology
Chemical and Biological Engineering
Date of birth
Sex
570123
Male
Academic title
Date of doctoral exam
Professor
1990-09-24
CO-OPERATING HEI
ENCLOSED APPENDICES
A, B, C, U, V, S
BUDGET
Funding period (planned start and end date)
2008-06-01 -- 2017-12-31
Funds applied for (kSEK)
2008
Linnéstöd och Berzelius Center
2009
2010
2011
2012
2013
2014
2015
2016
2017
2018
7500 7500 7500 7500 7500 7500 7500 7500 7500 7500
7500 7500 7500 7500 7500 7500 7500 7500 7500 7500
Total (kSEK):
Total amount for which applied (kSEK)
2008
2009
2010
2011
2012
2013
2014
2015
2016
2017
7500
7500
7500
7500
7500
7500
7500
7500
7500
7500
2018
POPULAR SCIENCE DESCRIPTION
Popularscience heading and description (max 4500 char)
(for translation into English see App. V)
Kemi är vetenskapen om molekyler, dvs partiklar som består av ett bestämt antal atomer som hålls ihop i väldefinierade strukturer
med starka bindningar. Kemins mål är att designa och syntetisera nya ämnen med användbara egenskaper, t.ex läkemedel eller
byggstenar
Vetenskapsrådet, SE-103 78 Stockholm, tel. +46 (0)8 546 44 000, [email protected]
Kod
2007-14950-54750-89
Name of Applicant
Markides, Karin
Date of birth
511124-1021
för avancerade material. Ofta har kemister nått sådana mål genom att kontrollera molekylernas inre struktur, men det kan vara lika
fruktbart att förstå hur bindningar mellan molekylerna kan utnyttjas för att ådstakomma de önskade egenskaperna. Svaga
inter-molekylära krafter är grunden för livets kemi, ty de behövs för den igenkänning som spontant ger upphov till välordnade
strukturer med avancerade funktioner, samtidigt som de tillåter den flexibilitet hos de uppkomna strukturerna som behövs för rörelse,
metabolism och reparation, typiska egenskaper hos biologiska system. Ett viktigt exempel är de selektiva intermolekylära
växelverkningar (basparning och basstapling) som säkerställer det genetiska budskapets lagring i DNA, samtidigt som de tillåter att
dubbelhelixen kan öppnas, vilket behövs när den genetiska information skall läsas av eller kopieras. DNA och andra inspirerande
exempel från biologin visar hur den samlade verkan av många svaga krafter kan utnyttjas för att spontant foga samman funktionella
supra-molekylära strukturer, dvs väldefinierad strukturer sammansatta av många molekyler som hålls ihop av de svaga krafterna. Vi
avser utnyttja syntetiska DNA-molekyler som byggstenar, där koden för igenkänningen mellan DNA-baserna (adenin passar ihop
med tymin och guanin med cytosin) används som en digital address så att olika byggstenar som förs in i strukturen kan hitta sina
rätta plaster. På så sätt kan, i princip, super-molekylära strukturer med önskad storlek, form och egenskaper designas efter behov,
och de kan byggas med hjälp av relativt enkla kodnings-algoritmer och korta DNA sekvenser som är programmerade att passa ihop
som bitarna i ett pussel. De DNA-baserade plattformarna är bara ett exempel på hur vi låter oss inspireras av biologiska strukturer.
Ett annat exempel är att efterlikna de lipid-membran som utgör den biologiska cellens hölje, och i vars yta man kan infoga molekyler
med olika funktioner och på så sätt konstruera en annan typ av supra-molekylära system. En möjlig användning av
supra-molekylära strukturer kan illustreras genom att hänvisa till utvecklingen av den digitala elektronik som används i
vardagstekniker som mobiltelefoner och GPS. Denna revolution har förlitat sig på en miniatyrisering av elektroniska kretsar, till dags
dato med hjälp av litografiska metoder. Nu börjar de minsta komponenterna närma sig den litografiska gränsen (i praktiken ca 100
nm, där 1 nanometer = 1 miljarddels meter), så det kommer snart att behövas ny teknologi som tillåter miniatyrisering på en ännu
finare nivå. Våra supramolekylära strukturer har storlekar på 1 – 100 nm, och skulle alltså kunna brygga gapet mellan nm-stora
molekyler och den litografiska dimensionen, vilket skulle vara av stort strategiskt värde. Ett möjligt exempel på supra-molekylära
strukturers användning i praktiken är hur nm-stora molekylära transistorer skulle kunna placeras ut på ett nano-chip med hjälp av
sina respektive DNA-adresser. Andra tillämpningar omfattar informationslagring, kemiska nano-reaktorer och omvandling av
solljusenergi, möjligheter som alla finns med bland de forskningsprojekt vi beskriver i ansökan. Vårt huvudmål är dock inte att
framställa ny teknologi, utan att undersöka och förstå, på en fundamental nivå, de växelverkningar och fenomen som nya
tillämpningar kan baseras på. En av många frågor vi avser undersöka är om igenkänning mellan molekyler måste baseras på hur bra
de passar ihop när de kommer samman, som nyckeln i låset, eller om andra egenskaper kan leda till igenkänning, såsom hur fort
molekylerna rör sig relativt varandra under bindningsprocessen.
Vetenskapsrådet, SE-103 78 Stockholm, tel. +46 (0)8 546 44 000, [email protected]
Kod
2007-14950-54750-89
Name of applicant
Markides, Karin
Date of birth
511124-1021
Title of research programme
Centre for Bio-inspired Supramolecular Function and Design
Appendix
A
Research programme
VRAPS/VR-Direct bilaga 2004.Ae
Vetenskapsrådet, SE-103 78 Stockholm, tel. +46 (0)8 546 44 000, [email protected]
Karin Markides 511124-1021
Supra Centre Chalmers
Appendix A
Centre for Bio-inspired
Supramolecular Function and Design
Summary
The project will focus on bio-inspired supramolecular systems for studying nanoscale chemical
and physical processes of biophysical and technological relevance. We will develop and study,
from fundamental, methodological as well as applied angles, systems based on nucleic acids or
hosted by lipid membranes, exploiting electronic properties and intermolecular recognition
mechanisms, to achieve controlled non-periodic structures with designed functionalities. Optical
spectroscopic methods are developed to gain detailed information about structure, dynamics and
electronic properties of the systems and their constituents, but also as basis for development of
new supra-molecular functionalities.
Unique competencies at the Chemistry and Physics Departments will be twinned in PhD
projects combining the participants’ current research interests. The Centre will define a long-term
strategic platform of generic tools for addressing applied problems: from micro-reactor and
enzyme technology, via medicinal and sensor applications, to bottom-up molecular nanotechnology, nano-optics and molecular electronics. One goal of the supra-molecular effort is to
bridge the gap between “chemical” (molecules) and “physical” (lithography) dimensions of nanoscience. As a result of close collaboration and integration of widely different competencies among
us we anticipate true synergism and new insights to be reached of great fundamental as well as
applied impact.
Karin Markides 511124-1021
Supra Centre Chalmers
Introduction. Development of supra-molecular
systems with designed functionalities, interfaced
with modern nanotechnology, provides a scientific
challenge with unique technological potential.
Particularly promising are small-scale molecular
structures and functionalities, inspired by
biologically perfected systems which often are
assembled by the collective action of many weak
intermolecular interactions. Here the molecular
sciences at the interface between chemistry,
physics and biology offer unprecedented
opportunities regarding theoretical and experimental methodologies to match the challenge of how to
produce new supramolecular systems and how to
control and exploit their properties for advanced
technical purposes. In chemistry and biology the
heart of function lies in specificity associated with
certain forms of molecular recognition, such as
DNA base-pairing, and this has also been the focus
in the field of bottom-up nanoscience based on
soft-matter self-assembly of molecules which has
emerged over the last ten years.
Goals and visions. Looking towards the coming
decade this project aims for biologically inspired
(regarding composition, structure and function)
supramolecular systems with controlled molecular
functionality based on deep understanding of
molecular interactions. Our multi-angle approach
combines competencies in the Departments of
Chemical & Biological Engineering and Applied
Physics to pursue cutting-edge research on
supramolecular function. The Centre will be
unique both regarding fundamental science and
strategic applications owing to its three
characteristic long-term targets:
1. To understand weak inter-molecular
interactions and exploit their potential to create
addressable supramolecular structures with
specific physical or chemical properties,
2. To develop methodology to study and
exploit electronic and optical interactions, both for
analyzing structure and dynamics of the
supramolecular systems, and as a basis for their
strategic functionalities,
Appendix A
3. To use these interactions to construct
supra-molecular structures that can bridge the gap
between the “chemical” (molecules) and
“physical” (lithography) dimensions of nanoscience.
The research consortium (see Table 1, p14)
represents expertise within the field of studying
and exploiting non-covalent interactions in
biological and other systems, as well as the
utilization of electromagnetic waves to investigate
systems controlled by such interactions. We have
also designed and commercialized devices and
instrumentation that can be used to study biomacromolecules and individual cells based on
these principles.
The proposal asks for support (SEK 7.5M/yr)
for research activities for the coming ten years, to
a group that currently consists of 10 principal
investigators with academic faculty positions and
an additional corresponding number of associated
researchers, to defray the cost of approximately the
same number of PhD students, arranged in pairs
with the purpose of twinning different activities of
the Centre. The research program deals with
fundamental curiosity-driven research on supramolecular systems involving nucleic acids,
peptides, lipid membranes, mesoporous materials
and synthetic polymers to build up a knowledge
platform relevant for addressing and interfacing
molecular and nano-technical applications in a
wide context.
We envisage a broad spectrum of
functionalities in the supramolecular systems we
have set out to study: from polymer solar cells,
molecular transistors, to mono-molecular catalysts,
microscopic chemical reactors and single-molecule
sensors. Our vision is that by the end of the ten
year period we shall have collectively:
1. Discovered general molecular mechanisms
/phenomena, of vital importance for the
emerging field of nano-technology,
2. Introduced experimental or theoretical
methodological procedures of text-book
impact,
3. Brought applications of new findings to patent
stage and commercial viability,
1
Karin Markides 511124-1021
Supra Centre Chalmers
4. Brought six junior researchers (three of whom
be women) to full professorships, one group
member recipient of the Göran Gustafsson
prize or similar international award. Within
three years five female candidates should
have emerged, qualified as research
associates or for replacing retiring PIs.
Background and an example. Nature provides
many examples of complex structures with a
variety of advanced functions: photosynthesis,
enzyme catalysis, energy conversion and storage,
molecular sensoring and signaling, membrane
pores as molecular filters, and the reading, copying
and editing functions associated with nucleic
acids, functionalities all of which we are prepared
to take inspiration from. A great deal of research is
already being devoted to analyzing and trying to
copy biological function for practical purposes.
Instead of copying biological systems, our
strategy will be to pursue fundamental and
methodological research, each of us having goals
set in concert with our respective individual
research field, to develop a self-contained generic
platform for addressing relevant bio-inspired
supramolecular systems. The familiar DNA double
helix may serve as an illustration. The biological
function of DNA, as well as its role as buildingblock in bottom-up nanoscience, is usually
ascribed to molecular recognition: adenine
recognizing thymine and guanine recognizing
cytosine by hydrogen bonds, providing the wellknown Watson-Crick double helix. It is important,
to note that the driving force to form a double
helix is the hydrophobicity of the bases that makes
them stack like a pile of coins on top of each other,
thereby excluding the competing water molecules
so that the hydrogen bonds can exert their full
force of recognition: the molecular recognition
only works if served by a framework of optimized
non-specific interactions. The electrostatic
repulsion between the phosphate-groups of the
DNA backbones is another necessary ingredient,
as evidenced by our studies of the nonionic
analogue peptide nucleic acid PNA.1 (In the
1
Wittung, BN et al Nature 368 (1994) 561.
Appendix A
references initials refer to members of the group
(Table 1), with principal investigators in bold.).
Thus, the rational design of supramolecular
structures clearly depends on an understanding of
the total array of weak inter-molecular forces.
The example illustrates key knowledge that
needs to be further refined to achieve the goals of
this proposal: how weak inter-molecular forces
determine structure and dynamics of supramolecular systems, how strong the complexes are
and how fast and by what mechanisms they
assemble/disassemble. Such knowledge allowed us
to invent PNA, as well as a protein-inspired
method to fuse selected vesicles with each other by
tagging them with complementary DNA strands2.
A prerequisite for such inventions is access to
powerful theoretical and experimental tools to
predict and analyze molecular structures and
intermediates. Those examples represent some
main facets of our proposal: to develop
fundamental understanding and methodological
skills to be able to design, produce and analyze
bio-inspired supramolecular structures with
generic properties suitable for a wide range of
applications.
Organization of the proposal. An overall
objective of our proposal is thus to reach
understanding for how complex molecular systems
with desired microscopic and macroscopic properties may be obtained and exploited. Below we
describe our planned fundamental and applied
research projects. Secondly, the significance of our
research background, anticipated synergisms and
relevant expertise demonstrated with examples of
cutting edge achievements is presented and set into
context.
A. Research activities
The research projects are divided into three areas.
Two of them, "DNA scaffolds" and "lipid host
systems" are closely related approaches used to
create platforms and mesoscopic structures which
2
Stengel, FH et al JACS 129 (2007) 9584.
2
Karin Markides 511124-1021
Supra Centre Chalmers
may be functionalized to bridge the moleculelithography gap. The third area, "Molecular
electronics", focuses on energy and charge transfer
processes by using these platforms, for both
fundamental research and applications.
I. DNA-based scaffolds for assembly of
molecular devices.
Background
The principle of DNA base pairing was first
brought into materials science by Seeman who
showed that DNA-based scaffolds can be used to
arrange individual metal particles in separated
rows.3 Using long single stranded DNA molecules,
Rothemund4 demonstrated that DNA-scaffolds can
be made addressable in a non-repetitive sense.
Scaffolds in the 100 nm range required singlestranded DNA molecules of sizes that are
produced biologically, which limits the number of
available sequences, and may lead to purification
problems in the scaling up to the amounts of DNA
needed in materials science. DNA scaffolds are
thus promising tools in nano-material science, but
in practice probably only in strategies based on
synthetic chemistry. Notably, synthetic trisoligonucleotidyls have been used to build
scaffolds.5
Aperiodic and addressable DNA structures
We will develop "addressable" supramolecular
structures using Watson-Crick DNA base pairing
as a digital code to produce aperiodic nanometersize arrays. The key building blocks are 3-way
branched oligo-nucleotides synthesized by an
orthogonal protection group strategy. In contrast to
the previously produced self-repetitive assemblies,
we shall make networks of DNA hexagons6, each
Appendix A
side of a hexagon containing a unique sequence
addressable by an incoming single strand of DNA.
Each edge of the hexagon corresponds
typically to one turn of a double helix (3.4 nm). A
central goal of our project is to bridge the gap
between this molecular length scale and the
dimensions available through lithography. The
first step towards this goal is to self-assemble a
network of several hexagons into a scaffold.
Secondly, such networks of DNA hexagons will be
assembled
onto
lithographically
produced
structures with mesoscopic dimension. Using
recently developed “click chemistry”, covalent
fixation of the assembled structures will be
achieved7. The click strategy will also be used in
other parts of this proposal.
Adressable DNA hexagons6 with unique sequences at
each edge, anchored to a lipid bilayer surface.
Lipid-assisted DNA-assembly
The individual DNA strands, currently being
hybridized in the bulk of an aqueous solution6, will
also be assembled on top of a lipid layer. Our goal
is to accelerate the rate of hybridization by forcing
the complementary oligonucleotides to move
along a surface. We have demonstrated that
oligonucleotides are able to hybridize when they
are anchored to a lipid bilayer8, so the fluid nature
of such lipid layers allows anchored reactants to
move and react. These observations puts us in a
unique position to investigate the fundamental
question how surface-confinement may catalyze
chemical reactions, for example the formation of
DNA scaffolds can be accelerated.
3
Seeman Nature 421 (2003) 427; Le et al Nano Lett 4 (2004)
2343.
4
Rothemund, Nature 440 (2006) 297.
5
Eckhardt et al Nature 420 (2002) 486.
6
Tumpane, BA, PL, BN et al Chem Phys Lett 440 (2007)
125.
7
Kumar, PL, Brown et al JACS 129 (2007) 6859; Kolb
et al Ang. Chem. Int. Ed 40 (2001) 2004.
8
Pfeiffer, FH. JACS 126 (2004) 10024.
3
Karin Markides 511124-1021
Supra Centre Chalmers
Kinetic recognition - a new concept with DNA
Kinetic selection effects in reactions that run far
from equilibrium, well-known from organic
synthesis, have recently also been demonstrated in
ligand-DNA contexts, with unprecedented
efficiency.9 Model systems based on binuclear
ruthenium compounds allow for a variety of
interactions to be varied and studied in detail,
providing important fundamental understanding of
structural and dynamic criteria for kinetic
selectivity. Kinetic recognition may have
advantages over “normal” (thermodynamic)
recognition in that it depends on other forces and,
as we have found, may probe the macromolecular
structure far beyond the size of the ligand binding
site. Fundamental research is motivated because
kinetic selection phenomena are important in a
general biomolecular context. Kinetic recognition
may also be exploited to achieve superior
addressability in the DNA based scaffolds.
A unified spectroscopic approach to evaluating
DNA scaffolds
Optical tools will be used and further developed to
study the assembled supramolecular structures and
their functionalisations. In particular we will use
radiationless resonance energy transfer (RET) to
gauge the structure dimensions, either using
organic chromophores (where RET acts at
distances of 1-10 nm)6 or metal nano sized clusters
(for distances 10-100 nm)10, built into or tethered
onto the assemblies.
Appendix A
The combined spectroscopic tool box of the
Centre thus covers the range of distances relevant
to our central goal of bridging the gap between the
molecular (nm) and lithographic (100nm)
domains. Additional structural information will be
obtained by using polarized-light spectroscopy
(linear dichroism) to probe the anisotropy of the
constructs11.
Mass-spectroscopy and CARS imaging
The RET-based methods to characterize the
supramolecular constructs have a unique range of
spatial resolutions, but the chemical specificity is
limited. We will therefore complement them with
mass-spectroscopy imaging, as recently used by us
to study membrane-lipid composition in cells and
in synthetic supported bilayers.12 Massspectrometry offers a unique opportunity to map
the chemical composition of the constructs at the
highly relevant spatial resolution of about 100 nm.
This potential will be used for imaging and
analyzing the constructs, as well as the underlying
lipid-composition in the case of membraneassisted assembly. The category of Non-Linear
Microscopy, employing optical processes as
CARS (coherent anti-Stokes Raman scattering),
SHG and THG (Second and Third Harmonic
Generation), offers non-invasive visualization of a
wide range of structural and chemical properties of
lipid based13 and other systems, at a comparable
resolution (~200 nm).
Nanoparticles as cellular sensors
Metal nano-clusters are such useful structure
probes because their active wavelength can be
tuned by choosing the right particle-size, and
because they enhance an incoming electromagnetic field without being bleached. These
advantages will be used also in several cell-based
projects. The enhanced electromagnetic field in the
nano-environment of the particles is ideal for
An array (top) of oriented silver particle-pairs gives the
nanostructured surface anisotropic optical properties.10
9
Nordell, BN, PL et al Ang. Chem. Int. Ed 46 (2007) 2203
LG, MK et al J Phys Chem B 109 (2005) 1079;
Svedberg, MK et al Nano Lett 6 (2006) 2639
10
11
Rodger, BN Circular Dichroism and Linear Dichroism,
Oxford University Press, 1997.
12
Ostrowski, AEw et al Science 305 (2004) 71; Prinz, FH
et al Langmuir 23 (2007) 8035.
13
Hellerer, AEn et al PNAS 104 (2007) 14568
4
Karin Markides 511124-1021
Supra Centre Chalmers
various cell-diagnostic methods, such as surface
enhanced fluorescence (SEF) or surface enhanced
Raman scattering (SERS).10,14
A promising application of cellular response
to heat-shock by targeted nanoparticles is
photodynamic therapy for cancer treatment, using
metal nanoparticles with strong absorption in the
NIR. We believe that the Centre will have a unique
potential to elucidate mechanism and improve this
approach, as we have the knowledge to design
particles with desired optical properties, access to
the time-resolved spectroscopic tools needed to
study their function, and the means to target the
particles to specific parts of the cell for both
diagnostic and therapeutic purposes.
II. Lipid-based host systems.
Background
Lipid bilayers play many roles in biology,
constituting various barriers to polar molecules in
the cell, including the plasma membrane, but lipid
membranes may also help to convey transport.
Recent observations15 indicate that cells can
communicate chemical substances through lipidbased tubes - a biological nanofluidic system!
Secondly lipid membranes act as hosts of
biochemical reactions. Membrane-bound enzymes
with hydrophilic substrates (e.g. ATP) are wellknown, but the membranes can also host
hydrophobic substrates for water-soluble enzymes
such as lipases. Chemically active biomembranes
(e.g. the endoplasmatic reticulum) often have
mesoscopic structures that resemble16 the folded
structures of bicontinuous cubic liquid crystals
formed by certain synthetic lipids and water.
Biology
thus
exploits
sophisticated
membrane geometries to catalyze reactiontransport processes in the cell. We take inspiration
from these evolved solutions, since we find them
14
Xu, MK Phys Rev Lett 93 (2004) 243002
Rustom et al Science 303 (2004) 1007; Önfelt et al J
Immunol. 173 (2004) 1511
16
Landh, FEBS Lett 369 (1995) 13; Deng et al J. Struct.
Biol. 127 (1999) 231; Zakaria et al J. Cell Biol.
173(2006) 839; Parton, Simons Nature Rev. 8(2007) 185
15
Appendix A
efficient compromises that maximize the catalytic
surface and minimizes transport retardation of
metabolites in the resulting contorted structures.
Objectives
Using such membrane geometries, produced in
artificial systems, we shall study and exploit
nanotube transport and catalysis of lipid-hosted
chemical reactions. We also intend to investigate
transport of molecules through the membrane
barrier, a long-standing challenge in cellular drug
uptake and transfection contexts, problems to
which several of our joint efforts are devoted.
The lipid-host projects demonstrate our
capacity to bridge the gap between the molecules
and the lithographic world. We have already
shown17 how lipid vesicle networks can be
arranged in a three-dimensional manner by use of
lithographic structures, a result which will be
useful in our attempt to organize DNA-hexagons
into mesoscopic scaffolds. Another example of the
bridge is found in the use of liquid crystals as hosts
for lipase-catalyzed reactions. Our goal is to
control the catalytic efficiency by precise
positioning and orientation of the enzyme and
substrate molecules at the water-lipid interface. In
addition, the overall efficiency will depend on the
rate of transport of reactants and products in the
pores of the mesoscopic liquid-crystal structures.
Chemical reactions & transport in nanovesiclenetworks
Nano-vesicle networks of liposomes connected by
lipid nanotubes will be used to study fundamental
effects of confinement on diffusion and chemical
reactions. A simple example is an enzymatic
reaction where substrate and protein initially are
housed in different liposomes connected to a third
vesicle ("reaction chamber") through separate
nanotubes. We have a good understanding of such
simple cases18 and now wish to explore how
changes in the confining geometry, such as several
spherical vesicles conjugated by conduits, will
17
18
Karlsson, AEw, OO et al Anal. Chem 78 (2006) 5960
Sott, ZK, OO et al Nano Lett 6 (2006) 209
5
Karin Markides 511124-1021
Supra Centre Chalmers
influence reaction dynamics. By (non-linear)
optical microscopy methods we shall study how
reactor geometry and topology influences the
dynamical reaction characteristics for various
reaction-diffusion wave properties (Dynamic
reaction control).
Chemical and biomimetic applications of
nanovesicle-networks
A second goal is to investigate if independent or
coupled reactions occurring in small-scale
networks may be used for selective synthetic
purposes or to create chemistry-based logical
devices or even be models to understand signaling
pathways in biology. The nano-vesicle approach
has several other interesting potentials: cell-free
synthesis of DNA or proteins in biomimetic
systems and for modulation-technological
applications (pulse-sequence control of chemical
reaction paths or frequency sensors), just to
mention two areas that we intend to explore. We
also wish to combine this research with cell
factory development and single-cell applications
with the goal of developing novel biotechnology.
Mechanisms and control of lipase-catalyzed
reactions in confined geometries
In the cell, lipases exert their action at the interface
between water and a lipid membrane. The active
site is based on a catalytic triad which is buried
beneath a “lid” that may fold open when the watersoluble enzyme approaches the hydrophobic
surface. The dynamic character of the active site is
expected to make lipases sensitive to the exact
positioning and orientation of the enzyme at the
interface.
Lipases retain their activity at the “oil-water”
interfaces of synthetic systems, such as nano-sized
water droplets in water-in-oil microemulsions or
surfactant liquid crystals.19 We will insert
representative lipases into nano-compartmentalized
media: water-in-oil microemulsions, lyotropic
liquid crystals of various geometries and water-
Appendix A
filled pores of mesoporous silica, in order to study
the effect of the environment on the enzymatic
selectivity. In particular we intend to investigate
the homologue discrimination, regioselectivity and
enantioselectivity of lipase-catalyzed reactions,
important aspects from preparative point of view
but not well understood.
Using esterases to convert ferulic acid into
lipophilic derivatives of medical interest
A related project concerns ferulic acid, an effective
antioxidant. In medical and functional food
applications antioxidation is needed in the unpolar
domains of living tissue, while the hydrophilic
ferulic acid tends to partition into the aqueous
domains. Lipophilic derivatives of ferulic acids
with retained and often improved antioxidant
function can be obtained by esterification with
aliphatic alcohols. Lipases may be used as catalysts
for this reaction, but the current efficiency is poor
because ferulic acid is too hydrophilic to be a good
substrate.
The efficiency of esterification can be
improved substantially by using feruloyl esterases
as the catalyst.20 This is a subclass of carboxylic
ester hydrolases that liberate phenolic acids, such
as ferulic acid, from naturally occurring
hemicelluloses by cleavage of an ester bond. Thus,
by acting in reverse, feruloyl esterases are the
perfect class of enzyme for our purpose. Using the
same type of microheterogeneous systems as
described above for lipase, the enzyme will be
present in the aqueous domain, and the feruloylalcohol product is expected to partition into the
unpolar domain. Isolation of an enzyme-free
product and reuse of the enzyme thus becomes
facilitated.
Molecular transport in confined geometries
The efficiency of amphiphilic liquid crystals as
hosts for enzyme-catalyzed reactions will depend
on the transport-rates of substrates, products and
enzyme molecules in the nano-porous structures.
We have studied the transport of both hydrophilic
19
KH Handbook of Microemulsion Science and Technology
(Eds. P.Kumar and K.L. Mittal) Marcel Dekker, 1999 p.713;
Reis, KH, Watzke et al. Langmuir 22 (2006) 8169.
20
Stevenson et al Enz Microb Technol 40 (2007) 1078;
Topakas et al. J. Biotechnol 102 (2003) 33
6
Karin Markides 511124-1021
Supra Centre Chalmers
molecules (oligonucleotides) and membrane-bound
species in synthetic lyotropic liquid crystals21, and
intend to extend those studies to metabolites and
proteins by combining spectroscopic and diffusionNMR-techniques.
We shall investigate how transport rates
depend on geometry and connectivity of the liquid
crystals, comparing lamellar, hexagonal and
bicontinuous cubic phases, and how they depend
on orientation of molecules in the membrane using
ruthenium complexes with variable orientations22.
The size of the headgroup and hydrophobic tail will
also be varied systematically with the goal to
develop new separation methods for amphiphilic
molecules, an important potential application.
Mapping the mesoscopic structure of liquid
crystals using DNA
DNA molecules are too large to enter the pores of
lyotropic liquid crystals, and remain in the aqueous
boundaries between micron-sized grains in the
polycrystals.23 We intend to use confocal
microscopy to image the 3D mesoscopic
polycrystalline structure by filling the grain
boundaries with dye-labelled DNA. In addition
Non Linear Microscopy will be used to map the
structural and chemical composition of the
polycrystals. Knowledge about grain size
distributions will be useful in catalytic applications
of liquid crystals (see above), and in their use as
templates for porous solids in materials science.24
Catalysis of transport, assembly and fusion at
lipid membrane surfaces
At the interface between the lipid and nucleic-acid
based research activities the controlled molecular
assembly and transport at lipid membranes (across
or laterally) provide an important platform that we
intend to pursue vigorously. Applications include
light- or voltage-gated ion channels, mechanical or
Appendix A
electrical sensors, nano-fabricated hole-spanning
membranes. Various scenarios mimicking cellular
membranes and transport driven by electric fields,
chemical reactions and gradients will be
considered as will technologies for DNA-induced
vesicle fusion.
Cell-penetrating peptides and transfection
In context of transport across membranes we will
explore mechanisms by which certain peptides can
conquer biological membrane barriers and gain
access to the cellular interior. They can be used as
vehicles for delivery of drug molecules or genetic
material to targets inside cells or in a biomimetic
context as transporters of essential signals. From a
cellular perspective therapeutic prospects for
siRNA, with the discovery of RNA interference25,
stand out as relevant applications for delivery
based on cell-penetrating agents. As to transfection
mechanisms both model and cell studies will be
made, on uptake of signaling substances, drugs or
genetic material confined in lipid membrane
vesicles tethered to cell-penetrating agents,
including peptides.26 For such complexes
endocytotic pathways for cell entry are expected
and mechanisms for subsequent endosomal escape
and release of vesicle contents into the cytoplasm
will be given high priority. Systematic studies of
interactions of lipid surfaces, and mediated or
directed lipid vesicle fusion, will be performed to
this end. Peptide- and DNA-assisted lipid
membrane fusion will also be explored as a
mechanism of transporting material into
nanovesicle networks and further inspiration for
specificity is envisaged from the nucleic acid
based fusion constructs developed within this
consortium. Fundamental mechanistic understanding of transmembrane transport, as well as of
peptide-mediated fusion events, can facilitate
development of new synthetic agents with superior
sustainability for future applications
21
Carlsson, BÅ, et al J. Phys. Chem. B109 (2005) 18268;
Langmuir 22 (2006) 4408
22
Ardhammar, PL, BN J. Phys. Chem. B 105 (2001) 11363
23
Svingen, BÅ J. Phys. Chem. B 108 (2004) 2735
24
AP Curr Op. Coll. Interf. Sci. 8 (2003) 145.
25
Fire Quart Rev Biophys 38 (2005) 303; Angw. Chem. Int.
Ed 46 (2007) 6966
26
Thorén, BN et al FEBS Letters 482 (2000) 265; Ceasar, PL,
BN et al Biochemistry 45 (2006) 7682,
7
Karin Markides 511124-1021
Supra Centre Chalmers
Nano-scale plasmonic apertures for studies of
membrane-translocation events
Ion transport across cell membranes is generally
studied electrically whereas translocation of larger
molecules is detected by fluorescence technique.
With our expertise in plasmonic active nano-scale
apertures27, and the possibility to self-assembly
supported lipid membranes in such structures28, we
have a unique opportunity to address material
transport across membranes in a fundamentally
new way. The plasmonic activity of nano-scale
apertures in thin metal films enables probing of
both binding to and transport across the
membranes, based on changes in interfacial
refractive
index.
In
combination
with
fluorescence-labelling of the involved entities
(lipids, peptides, membrane residing receptors,
ligands etc), fluorescence enhancement effects will
be possible to utilize with unprecedented
precision. By integrating these devices with
impedance spectroscopy, reactions probed by
optical means may be directly correlated with their
charge-translocation events.
Permeability of cellular membranes
Product toxicity often limits production in
fermentation processes. For example, it is well
known that yeast produces ethanol to levels where
this metabolite first hampers growth and later also
prevents continued metabolism.29 The mechanism
of ethanol toxicity is, however, not well
understood30 and this limits strategies for
improving the tolerance towards ethanol, as well as
other metabolites known to be harmful. As
industrial applications of cell factories calls for
high titers and high productivity, fundamental
understanding of the interaction between small
molecules and membranes is necessary for future
development of cell factories.
In model lipid membrane systems ethanol
can cause membrane perturbations leading to
27
Dahlin, MK, FH, JACS 127 ( 2005) 5043
Jonsson, FH et al Nano Letters in press DOI:
10.1021/nl072006t
29
Devantier, LO et al Biotech Bioengin 90 (2005) 703
30
Jeffries, Jin Adv Appl Microbiol. 47 (2000) 221
28
Appendix A
increased permeability and leakage. To what
extent such mechanisms also apply for true
biological membranes which encompass a large
fraction of molecules with stabilizing functions
including sterols and proteins is not known. We
will use membranes from whole cell preparations,
as well as artificial membrane mimicks, to study
the influence of a selected set of chemical
compounds (naturally occurring metabolites and
desired products of overproduction) on the
function and structure of the cell membrane. We
will investigate to which extents/rates those
molecules bind to, solubilize in or penetrate the
membrane, and how those effects depend on the
membrane composition. Important tools will be
established methods of our consortium including
quartz micro balance for adsorption and membrane
and
various
viscoelasticity
assessment31
spectroscopies to study leakage, orientation and
membrane perturbation
Delivery of lipids and membrane-residing
compounds to preformed lipid membranes
A method using DNA for programmed vesicle
fusion2 (inspired by a protein-based machinery
responsible for membrane fusion in nature), is
anticipated to become useful in several projects.
More specifically, it will be used for designing
DNA for programmed fusion of proteoliposomes
to nano-aperture-spanning membranes and vesicle
networks. This approach could provide insertion of
arbitrary membrane-residing proteins; and also
provide a means for post-modification of the
membrane composition and subsequent studies of
the so induced changes in membrane-protein
function in both artificial vesicles and cells.
III. Molecular electronics
Background
The communication between the various parts
an assembled structure is a feature in common
any complex device, whether for conversion
solar energy to chemical/electrical energy,
31
AC, FH, AE, OO et al Anal Chem 73 (2001) 5805; FH,
Elwing et al Anal. Chem 73 (2001) 5796.
8
of
of
of
or
Karin Markides 511124-1021
Supra Centre Chalmers
networks of neurons or electronics of computors.
Gating and switching of complex pathways of
(electro)chemical or optical communications are
used to store, process and retrieve information. As
macroscopic scale laws break down, the
miniaturization of electronics will at a certain level
require a "quantum leap" into what can be
envisaged as a molecularisation of electronics.32
Our strategy is a concerted effort to bridge the gap
between the nanometer and the macromolecular
levels, both in spatial construction of devices as
well as their temporal operation, to address the
communication problem in a wide scope.
Molecular electronics – designing molecular
components with controlled properties
The transport of electrons and excitation energy is
studied in model systems to create a platform for
applications such as organic solar cells and
molecular-scale electronics. A model system
typically is a molecule that has been designed and
synthesized to have specific properties when
placed in a well-defined environment (solvent,
DNA, membrane). It is studied with ultra-fast laser
spectroscopy to determine the mechanism(s) by
which excitation energy is transferred or converted
into electron migration. In parallel quantum mechanical calculations are used to model the transfer
processes, to generalize the knowledge and guide
the development of new theoretical models. The
fundamental knowledge is applied in the
development of materials were electron transfer is
the key reaction.
Conformational control of electron transfer – a potential
molecular switch. The electron transfer from a
porphyrin dimer (left) to a fullerene acceptor (right) is
sensitive to the dimer conformation, which be tuned by
by selecting the wavelength of excitation.33
Appendix A
This project will explore the possibility to use
controlled "vectorial" excitation energy transfer
(EET) and electron transfer (ET) for the
development of molecular scale electronics. We
have developed solid understanding of how the socalled electronic coupling can be tuned in
molecular structures.34 Computations have allowed
us to model the different transfer reactions and are
now developed to a level where they could be used
to predict the performance of molecular electronic
components.
We intend to extend this knowledge platform
towards realization of molecular scale electronics.
Specifically, we shall develop molecular wires,
switches, investigate molecular logical functions
and study the molecule-to-metal contact problem.
In addition, the various applications of organic
solar-cells (see below) will connect to the
fundamentals from this project.
DNA Lithography
The realisation of the next step in the nanoscience
revolution would be to bridge the molecular and
lithographic dimensions. This would enable us to
assemble bottom-up designed molecular systems
and connect with the top-down etched structures of
contemporary electronics. While standard photolithography is limited by the Abbe resolution, i. e.
typically 100 nm for light with wavelength 300
nm, lithographic patterns may be created from our
DNA network onto a suitable substrate with much
higher resolution. A pattern of the DNA network
may be “printed” on the surface with sub-nm
precision using photo-induced reactions at
molecular level. After transferring the pattern to
the surface the DNA network can be utilized again
at a new location on the surface - a "stamp" idea
that we wish to pursue. Some crucial steps in this
procedure have already been tested in other
contexts, such as the functionalization of a network
with photo-redox active components, and
demonstration of vectorial photoinduced electron
34
32
33
Joachim et al Nature 408 (2000) 541
Winters, BA et al Chem. Eur. J. 13 (2007) 7385
Kilså-Jensen, JM, BA et al JACS 123 (2001) 3069;
Winters, JM, BA et al Chem. Eur. J. 11(2005) 562; Eng, BA
Angew. Chem. Int. Ed. 45 (2006) 5626; Wiberg, JM, BA et al
JACS 129 (2007) 155.
9
Karin Markides 511124-1021
Supra Centre Chalmers
transfer from a network-bound molecule to a lipidphase-bound electron acceptor.
Beyond Förster: resonance energy transfer
through plasmonic interactions
Resonance Energy Transfer35 (RET) processes
among molecules are ubiquitous in nature, but their
technical applications are limited by the short
Förster distance (typically 1-10 nm). We will
attempt to move beyond the spatial range of
molecular RET by utilizing plasmonic metal nanostructures as antennas for the acceptor-donor
interaction. Such a tool is central to our goal to
bridge the gap between molecular and lithographic
length
scales.
From
cavity
quantum
electrodynamics and pioneering experiments by
collaborators36, we know that RET is determined
by the local photon density of states (PDOS),
which is strongly modified by plasmons. Based on
electrodynamics simulations the PDOS will be
engineered through lithographic design of gold or
silver nano-architectures, to coincide with the
molecular transition energies of appropriate RET
pairs. The latter will be fixed at rigid (addressable)
DNA structures at predetermined positions and,
using for instance thiol chemistry, at specific
distances from the metal surface. This will allow us
to quantify the transfer efficiency as a function of
donor-acceptor and metal-molecule distance using
fluorescence life-time imaging and to model the
system within the theory for plasmon-molecule
interactions37. A goal will be an enhancement of
the Förster distance by an order of magnitude
(>100 nm). This achievement will have great
impact on a variety of applications, such as solar
energy conversion, development of light emitting
diodes as well as methodology for studying
biomolecular structure and interactions using RET.
35
G.D. Scholes, Annu. Rev. Phys. Chem. 54 (2003) 57
Andrew, Barnes Science 306 (2004) 1002
37
Johansson, MK et al Phys. Rev. Lett. 93 (2004) 243002;
Phys. Rev. B 72 (2005) 035427
36
Appendix A
Energy- and electron-transfer – control and
applications
We will thus use the DNA- and lipid-based
platforms to study mechanisms of energy and
electron transfer between molecules, and also
nanoparticles, kept at well-defined relative
positions and orientations by tethering to the
DNA-scaffold. We will also explore the potential
of plasmon resonances as electron transfer
pathways; the main non-radiative decay channel of
the nano-particle plasmon is into (hot) electronhole pairs, either in the nanoparticle itself or in an
adjacent excitable medium (molecule or
semiconductor). We are inspired by the lightharvesting and charge-separation steps in
photosynthesis, but aim at designing our own
solutions through fundamental studies under wellcontrolled geometric and energetic conditions, by
combining experiment and theory.
Our uniquely addressable DNA scaffolds
allow us to study geometry effects on resonance
energy transfer (RET), both on short (10 nm) and
long (100 nm) distances, using organic
chromophores and metal-particles, respectively.
The question how surface plasmons enhance RET
between metal-clusters at well-defined distances is
an interesting research field in its own right, but
also has bearings on a second aim of designing
artificial arrays of molecules to harvest solar
radiation energy for photo-voltaic function. In this
application electron transfer and charge separation
is the crucial step, as in photosynthesis. Our
studies34 of electron/hole creation and transport in
bridged porphyrin-systems have demonstrated the
role of geometry, but also revealed the importance
of the energies of the electronic states of the bridge
between donator and acceptor.
By placing similar chromophores and redox
centers at predetermined addresses on the DNAnetwork we shall study various photo-induced
functions on the 1-100 nm length scale, including
excitation energy migration (i.e. light harvesting),
sequential electron transfer, followed by charge
separation (i.e. an artificial reaction center).
Tethering to the DNA scaffold may allow
geometry- and energy-factors to be studied
independently. Other interesting possibilities
10
Karin Markides 511124-1021
Supra Centre Chalmers
include design of systems capable of either
mediating charge over long distances or act as
molecular insulators, i. e. prerequisites for
"molecular electronics". Here also our design and
manufacturing of semiconducting polymers comes
into context, for the long-term aims of solar cells,
molecular sensors, and other molecular electronics
applications.
New molecular photo energy converters
One applied goal is molecule-based solar-energy
converters, the principle of which is simple: a
molecule with suitable absorption properties with
respect to the solar spectrum absorbs a photon
which brings it to a high-energy excited state. The
excitation energy is used to drive an electron,
either from the original (donor) molecule or from
some other molecule to which the energy has
been transferred, via one or several
acceptor/donor molecules to an electrode. From
an opposite electrode an electron is delivered
back to the donor molecule and the current
through an external circuit connecting the two
electrodes can carry out work or be used to
electrolyze water to produce hydrogen fuel.
Efficiency is limited by various ways by which
excitation energy or electrons may dissipate.
Model of a photo-active ruthenium-DNA complex.
Our idea to prevent electron back-donation is to
exploit electrostatic repulsion to make a semiquinone
radical anion (carrying the electron) swing out from
the negatively charged phosphodiester backbone of
DNA, away from the photo-electric donor.
We shall focus on how to prevent backreactions by designing molecular systems in
which the electron (or its carrier) is rapidly
brought away from the donor molecule.38,39
Polymer solar cells
Solar cells can be prepared from semiconducting
conjugated polymers. The cells are made of
combinations of molecules and nanoparticles.
Normally, the active substance consists of two
materials with different electron affinity: one that
transports holes and another that transports
electrons. In this project we will focus on polymerand
polymer-nanoparticle41
polymer40
combinations. So far such solar cells are prepared
without real control of blend morphology. To
obtain high efficiency in the cells the morphology
of the active material must be controlled.
Introducing weak interactions between materials
will be a tool to modify the morphology. Polymers
with attached functional sidegroups for controlling
the supramolecular structure of the active material
will be prepared. The blend should have a
controlled morphology with domain sizes below
10 nm and two co-continuous phases (for the
charge transport). By varying the kind and amount
of functional groups the degree of interactions
between the molecules could be tuned within wide
limits. The resulting material will be studied in
detail as its solar cell performance will depend on
both electronic properties and morphology. Nanoparticles (ZnO, TiO2….) of varied size and surface
treatment will be prepared. For the function of the
solar cells other demands on the materials have to
be met such as high stability, high mobility,
absorption covering the important parts of the solar
emission, controlled and fine-tuned HOMO and
LUMO positions of the polymers. We have long
experience in this area. As a tool for designing
new materials we will also use modeling methods.
The energy and electron transfer rates are studied
by optical spectroscopy.
The solar cell polymers as well as the photoactive ruthenium complexes will also be studied in
mesoscopic ZnO nano-wires as well as lipid-or
DNA-based systems as hosts for the photo-active
molecules.
38
40
39
41
Önfelt, PL, BN, Zewail et al PNAS 97 (2000) 5708
Westerlund, BN, PL et al. J. Phys. Chem. B 109 (2005)
17327
Appendix A
McNeill et al Appl Phys Lett 90 (2007) 193506
Beek et al J. Mater. Chem. 15 (2005) 2985
11
Karin Markides 511124-1021
Supra Centre Chalmers
B. Our environment and synergisms
Many of the PIs have collaborated over the
years and share common research themes. DNA as
a template for nanostructures, electronic materials,
theoretical and experimental studies of electron
transfer, lipid layers on surfaces and in vesicle
networks, as well as quantum dots are all problem
areas that have been addressed by team members,
sometimes in collaboration but often from different
angles. Thus, the chemists and physicists in the
group can be expected to work smoothly together
on the problems we address because we have a
common scientific language - at the same time our
different competencies will complement each
other.
The Supra Centre constellation is therefore
anticipated to give strong synergistic effects, as
exemplified by the following examples of our
common goals.
• Develop interfaces between supramolecular
constructs and lithographic structures, by
combining chemical and physical approaches.
• Formulate common principles for resonance
energy transfer in molecules, metal particles
and lithographic structures.
• Combine time- and mass-resolved spectroscopy
with AFM and CARS-microscopy, in order to
study the structure and dynamics of surfaceconfined systems.
• Apply those principles and methods to
characterise the geometry, composition and
function of DNA and lipid-based scaffolds at
length scales between 1 and 100 nm.
• Combine chemistry, micro-manipulation and
lithography to build three-dimensional
networks of biomimetic vesicles, and
functionalise them by addressed vesicle
delivery of enzymes and other agents.
• Establish principles for how chemical reactions
and transport of reactants and products are
coupled in membrane-confined geometries
and 2D-fluids.
Appendix A
• Develop molecular, quantum dot and lithographic electron transfer systems, and
theoretical models for their understanding.
Through research efforts described in more detail
below, we have acquired international recognition
in our respective fields. Thus, unique competence
is found in advanced optical spectroscopy and
photophysics, in micro-fluidic science and lipidbased reaction/transport systems, as well as in
electron transfer processes and conducting
polymers, just to mention a few areas of specific
benefit for the project.
A crude indicator of impact of research is
provided by citation records. Many of the senior
PIs have extremely high records of seminal papers
published relatively long ago (one publication of
1982 belongs to the most cited publications in
Sweden). However, also when confining the
statistics to the last 10 years, the 10 PIs have
together published more than 800 papers in mostly
high profile chemistry and physics journals, cited
more than 10000 times. Several team members
have also been active filing patents, some of which
have resulted in sucessful start-up companies.42
Many team members have received
prestigious awards or grants in the highest possible
national competition, including two Göran
Gustafsson Prizes, several Senior and Junior
Individual Grants from the Foundation for
Strategic Research (SSF), as well as appointments
as Senior Research Fellows by both the Swedish
Research Council (VR) and the Royal Swedish
Academy of Sciences. These awards underline the
considerable individual competencies that are
brought together in the consortium.
The group has organized numerous
conferences on a variety of topics of relevance for
the proposal. Specifically, recently Nordén and
Orwar have been initiators and organizers of
several Nobel Symposia and Workshops such as
"Energy in Cosmos, Molecules and Man", “The
Chemical Origin of Life” and “Nanoscale Motion”,
and Albinsson will be the Chairman for the XXIInd
IUPAC Symposium on Photochemistry to be held
42
www.q-sense.com; www.cellectricon.se
12
Karin Markides 511124-1021
Supra Centre Chalmers
in Göteborg July 2008. Several members of the
group are active in research policy bodies
nationally and internationally. For example,
Nordén is the chairman of the Council of the
European Research Councils Chemistry Committees (CERC3) and President of the Chemistry
Section of the Royal Swedish Academy of Science.
Holmberg was President of the Swedish Chemical
Society between 1999 and 2005, Chairman of
Chemical Engineering at the Royal Academy of
Engineering Sciences (IVA) during 2001-2004 and
President of the International Association of
Colloid and Interface Scientists during 1996-1998.
Albinsson is the chairman of the theoretical and
physical chemistry group of VR and the Swedish
representative in the Domain Committee for
Chemistry, Molecular Sciences and Technology in
the COST program of the European Science
Foundation.
Another indicator relevant for the proposal is
the research training record, with near 100 PhD:s
graduated from Chalmers during the last 10 years
under our supervision. We consider undergraduate
as well as graduate education as an important,
integrated part of our research. Over the years the
research environments of our Departments have
fostered many highly skilled scientists that today
have important positions in industry or academia.
The research training of PhD students is given high
priority, combining scientific depth and general
knowledge, as it provides not only individuals
attractive on the job market, but is also a
prerequisite for creativity and research of high
quality.
Appendix A
is the collaboration with oligonucleotide-synthetic
group of Tom Brown, Southampton University6
(The Centre itself houses extensive synthetic
expertise through JM and NK).
Current international collaborations include seven
EU-projects:
1) AMNA (coordinated from Physical Chemistry,
Chalmers) aiming for addressable nucleic acid
network of nanometer dimensions
2) NEONUCLEI aiming for development of
prototype synthetic cell nuclei
3) NANOBIOMAPS: TOF-SIMS imaging of lipid
model systems, cells and tissue
4) SNIPER gene-targeting strategies
5) ZNIP therapeutic in vivo DNA repair by sitespecific double strand breaks
6) Plasmo-Nano-Devices, aiming at sub-wavelength
miniaturization of optical interconnects and
photonic components
7) PHOREMOST - photons to realize molecular
scale technologies
We intend to collaborate with S. Zhang (MIT) who
has expressed a strong interest in our proposed
research. His synthetic oligopeptides44 have a
broad set of designed interactions that complement
the
hydrogen-bonding
scheme
of
DNA
basepairing.
Nationally, ongoing research programs
include
1) Nano-X, Novel nanoscale soft-matter devices,
(Foundation for Strategic Research SSF)
2) Solar cells and solar fuels (Knut and Alice
Wallenberg foundation)
3) Colloid and Interface Technology (SSF)
4) Competence Center for Catalysis (the Swedish
Energy Agency)
5) Biomimetic Materials Science Program (SSF)
6) Supramolecular Biomaterials (VINNOVA)
7) Marine Paint (Swedish Foundation for
Environmental Research)
8) Ytkemi för plast- och kemiindustrin, a national
graduate school in surface chemistry (the Swedish
Foundation for Knowledge and Competence)
9) National graduate school in material science. (SSF)
National and international collaborations
Members of the group are involved in collaborative
activities of various kinds. The international
collaborations involve world-leading groups in
areas that we pursue, such as bioinspired selfassembling constructs (Lehn)6, reactions in lipidconfined nanogeometries (Zare)43 and ultrafast
energy conversion in chemical reactions
(Zewail).38 Of particular importance for this project
In addition to our academic colleagues we have
extensive collaborations with industrial partners in
43
44
Chiu, OO, Zare et al Science 283 (1999) 1892; Strömberg,
OO, Zare et al PNAS 97 (2000) 7
Zhang Mater. Today 5(2003) 20; Nature Biotechnol. 21
(2003) 1171
13
Karin Markides 511124-1021
Supra Centre Chalmers
Sweden and abroad. Nationally these include Akzo
Nobel Surfactants, AstraZeneca, BIM Kemi,
Carlfors Bruk, Eka Chemicals and Södra Cell.
Internationally, we have projects with BASF,
Germany, Nestlé, Switzerland and Oxiteno, Brazil.
Group organization
The members of the group represent mutually
complementary, cutting-edge competence central
for the project, all communicating in a common
physical-chemical language. In addition to the 10
PIs listed in the application, another 10 scientists
(see Table 1) with long-term academic positions at
Chalmers are engaged through collaborations.
Table 1. Participating scientists (2007)*
Bo Albinsson (m44)
Professor, PI
Mats Andersson (m41) Professor, PI
Ann-Sofie Cans (f36)
Assistant professor
¤
Annika Enejder (f38)
Associate professor
Andrew Ewing# (m50) Professor
Linda Gunnarsson (f35) Assistant professor PI
Krister Holmberg§ (m61) Professor, PI
Fredrik Höök§ (m41)
Professor, PI
Nina Kann (f43)
Associate professor
Zoran Konkoli (m41)
Associate professor
Professor, PI
Mikael Käll§ (m44)
Sven Larsson (m66)
Professor
Per Lincoln (m49)
Associate professor
Jerker Mårtensson (m42) Associate professor
Bengt Nordén (m62)
Professor, PI
Lisbeth Olsson (f44)
Professor, PI
Owe Orwar (m43)
Professor, PI
Anders Palmquist (m41) Professor
Sofia Svedhem (f37)
Assistant professor
Björn Åkerman (m50)
Professor, PI
* Gender and age in paranthesis.
¤
Abbreviated AEn
#
Marie Curie Chair, Analytical Chemistry, Göteborg
University. Abbreviated AEw
§
These PI participate in a second non-ovelapping program
which forms a valuable complement (cf Appd U)
In addition, a number of promising young
scientists, directly or indirectly involved in the
projects (as shown by our citations), will be
considered for recruitment as faculties. Among
these candidates we count Alexander Dmitriev,
Appendix A
Elin Esbjörner, Kristine Kilså-Jensen, Jan
Lagerwall, Tatsiana Lobovkina, Gudrun Stengel,
Pernilla Wittung.
The groups have at present together 50 PhD
students, 10 post-docs and 2 visiting professors
involved in activities that would fit well into the
overall theme of the Centre. The 20 involved
scientists all work according to goals set by
themselves and it is our ambition that the situation
will remain so. This is necessary if scientific
excellence in the group will be maintained and
developed, in particular for the younger members
of the group. Nevertheless, the joint projects
described in this application will be promoted
through the fruitful combination of complementary
competencies. Most of the PIs have already
collaborated on topics of relevance for the project,
as documented by joint scientific publications. In
order to further promote interdisciplinary
exchange, 10 PhD students will as pairs address
related problems from different angles: e.g. one
chemistry student collaborating with a physics
student. A unique feature of the program is the
close contact between theory, experiment and
application. Seminars and PhD graduate courses to
further strengthen this feature will be at the core of
our activities. The budget also includes resources
to invite guest lecturers and members of a
scientific advisory board.
Experimental resources
Chalmers and Göteborg is very well equipped
regarding experimental resources needed for this
project. Thanks to big instrument grants from the
Swedish Research Council and several private
Foundations, about two major investments have
been made every year for many years at the
Chemistry and Physics Departments. Today our
resources include modern ultra-fast laser
spectroscopy, atom-force and electron microscopy,
mass spectrometry, advanced microspectroscopy
(Swegene Centre for Biophysical Imaging) and
state-of-the-art
facilities
for
cleanroom
micro/nanofabrication and characterisation (MC2)
as well as nuclear magnetic resonance (the
National NMR centre being in Gothenburg).
14
Karin Markides 511124-1021
Supra Centre Chalmers
Appendix A
Presently, Chalmers is making a large investment
in life science and a strong experimental platform
in fermentation technology and quantitative
systems biology (involving LO) will be established
during 2008.
While we are well equipped with advanced
physical instrumentation and theoretical tools
suitable for addressing our tasks, we anticipate
encountering some severe challenges as our
molecular systems get more complicated. Our
strategies as well as systems have a hierarchic
character: design and synthesis of modular
molecular building blocks earmarked to selforganize into gradually bigger complexes,
eventually reaching macroscopic dimensions. Here
thermodynamic and kinetic control of assembly
and circumventing solubility problems will
provide major challenges as will, of course, the
various
advanced
functionalities.
Also
requirements of "addressability" and high
structural fidelity will present challenges to the
design of building blocks and their later permanent
fixations to each other.
C. Our research background
Rejuvenation - and retiring scientists
Ten years project time means that the current crew
of senior scientists will be gradually succeeded by
younger ones. However, another urgent reason for
renewal is the need to respond to the incessant
move of the research front. Thus, already from
start we plan to recruit (for three years) three
postdoctoral research associates and to consider
additional possibilities for tenure track positions to
connect with the project, in order to get young
scientists to bring new skills and new perspectives.
Adressable DNA-based supramolecular systems
We have successfully initiated a project for
building hexagonal networks of DNA.46
A summary of previous achievements of the
group, focusing on the scientific results which are
relevant for the present proposal.
Polarized-light spectroscopy.
We have a world-leading position in linear and
circular dichroism spectroscopy for studying
molecular structure and electronic properties
(transition moments) of macroscopically oriented
systems.45 Methods developed by us include
applications to nucleic acid-ligand and protein
interactions, e.g. structure determination by
chromophore replacement through site-directed
mutagenesis in systems not amenable to
crystallography or NMR analysis.
Lipid membranes are also possible to study
with this technique in the form of shear-aligned
lipid vesicles of 100 nm size, which may be
studied both with respect to their own structure
and dynamic behaviour in shear-flow as well as
the positioning, orientation and conformation of
proteins and other guest molecules.22,26
Leadership
A steering group of three persons will be
responsible for policy, resource allocation and
reports to Chalmers and VR. For the first threeyear period the group will consist of Linda
Gunnarson, Bengt Nordén (chairman) and Lisbeth
Olsson.
Nanometer-sized hexagons formed by selfassembly of six oligonucleotides, giving each
edge a unique sequence.46
45
BN et al Quart Rev Biophys 25 (1992) 51; Frykholm, BN
et al Biochemistry 45 (2006) 1172.
46
Tumpane, BA, PL, BN et al Chem Phys Lett 440 (2007)
125
15
Karin Markides 511124-1021
Supra Centre Chalmers
Each side in a hexagon contains a unique
sequence, meaning it is addressable by an
incoming single-triplex strand of DNA, and
typically corresponding to one turn of a doublehelix (3.4 nm). We have recently demonstrated
this triplex strategy in the corresponding
"naphthalene"-type structure. 47
Manipulating DNA/RNA
We have manipulated DNA at various levels:
from modifying the phosphate-ribose backbone
or the nucleobases, to condensing large DNA
using aminodendrimers as histone mimicks or
aligning the DNA helix in a flow gradient or by
“migrative orientation” where we were first to
report the observation of electrophoretic orientation. We hold patents for the artificial DNA
construct peptide nucleic acid (PNA), its
diagnostic and nano-technical applications, and
patent for biomedical and nano-technical
applications of binuclear DNA-binding metal
complexes.48,1 The latter type of compounds
have recently enabled discovery of a new
recognition mechanism of long AT sequences of
DNA based on kinetic selection.9 We have also
shown that tricyclic DNA base analog tC is
brightly fluorescent and may be built into DNA
without significantly distorting its structure.
Recently this allowed us to do a time-resolved
conformational dynamics study of a DNA
polymerase in action49, a project that is to be
extended to the single-molecule level
Nanotube-vesicle networks
We have developed techniques for creating
complex networks of micron-sized containers
connected by nanotubes, all formed by liquid
crystalline lipid bilayers in water. The propensity
of such materials to undergo complex shape47
Tumpane, BA, PL, BN et al, Nano Lett accepted
Nielsen, BN et al Nature, 365 (1993) 566; Önfelt, PL,
BN et al. JACS 123 (2001) 3630; BN, BÅ et al Quart
Rev Biophys 24 (1991) 103;
49
Engman, PL, BN, BA, Wilhelmsson et al Nucl. Acid
Res. 32 (2004) 5087; Stengel, BA, BN, Millar et al
Biochemistry 43 (2007) 12289.
48
Appendix A
transitions under mechanical and chemical
excitations is exploited to form two- and threedimensional networks.50
An attractive feature of these nanotubevesicle networks (NVNs), apart from their small
size and their facile integration to lithographic
substrates, is that they can accommodate proteinbased functionalities of catalysis or responses to
light, chemical or mechanical stimuli. The desired
functions can be allocated to any given node
(container) within the network, allowing us to
design complex devices on the nm- to-µm scale.
We have used the micron-scale NVNs to
accomplish well-controlled and rapid mixing of
reactants by diffusion, for studies of chemical
reaction dynamics in confined geometries.51
A network of lipid vesicles and nanotubes
assembled on a solid state litographic substrate.51
Dynamic liquid film formation
We have established a technique to spread lipid
monolayer films on patterned hydrophobic
surfaces made from the photoresist SU-8. The
patterns made by direct electron beam writing can
reach nanometre length scales, and molecularly
thin films with designed functionalities can be
patterned onto such structures.52 In addition
different lipid films can be mixed under
stoichiometric conditions, so that reactions can be
50
Karlsson, AC, OO et al Nature 409 (2001) 150; Ann. Rev.
Phys. Chem. 55 (2004) 613; PNAS 18 (2002) 11573
51
Karlsson, AEw, OO et al Anal. Chem 78 (2006) 5960
52
Czolkos, OO et al Nano Letters 7 (2007) 1980
16
Karin Markides 511124-1021
Supra Centre Chalmers
initiated at will. In effect, these surface-assisted
supramolecular assemblies function as 2D microor nanofluidic devices. We have also demonstrated
the facile immobilization of cholesterol-modified
oligonucleotides onto the same type of surfaces,
and in a state that allows for hybridisation of an
incoming complement. The unique 2D-nature of
the surface-reactors was demonstrated by the
release of the oligonucleotides by a spreading lipid
film.
Lipid membrane function
We have developed spectroscopic methodology
and theoretical models to study how rutheniumcomplexes and peptides interact with membrane
surfaces of lipid vesicles, in particular regarding
the affinity, conformation and orientation of these
molecules in the membrane. We have also
developed methodology to study transport across
the vesicular membranes, based on resonance
energy transfer. For instance, the mechanism of
cellular uptake of “cell-penetrating peptides” has
been studied in detail for lipid vesicle membrane
models, as well as in live cells. One important
result is that cationic peptides overcome the Born
energy barrier to membrane-translocation by ionpairing with phospholipids.53
As a spin-off, our careful characterization of
how ruthenium-compounds bind to the membrane
allowed us to use them as optical probes in
polarized spectroscopy, in order to study vesicle
deformation and lipid packing.22
Reactions in confined media
In the area of organic and bioorganic
reactions in confined media, we have utilised the
tendency for amphiphilic molecules to selfassemble into specific structures, usually with an
aqueous domain coexisting with a nonpolar
domain, and used such structures as reaction
media. The microheterogeneous media include
53
Thorén, BN et al Biochem Biophys Res Comm 307 (2003)
100; Biochemistry 43(2004)34, Esbjörner, BN et al Biochem
Biophys Res Comm 1768 (2007) 1550. Esbjörner, BN et al J.
Phys. Chem. B111 (2007) 10839
Appendix A
microemulsions with either water-in-oil or
bicontinuous structure, microemulsions in
equilibrium with one or two excess phases,
lyotropic liquid crystals and suspensions of
inorganic mesoporous materials, prepared via
self-assembled surfactants as templates.
The investigated cases have been different
types of non-catalyzed organic reactions in which
there is a reactant compatibility problem that can
be solved by the use of the microheterogeneous
media with a very large interface between the
polar and the nonpolar domains. Reactions have
also been performed with a homogeneous
catalyst - a noble metal - deposited in the pores of
a mesoporous material.
A lipophilic reactant, 4-tert-butylbenzyl bromide, meets
a hydrophilic reactant, an iodide ion, at the oil-water
interface of a microemulsion.54
Finally, reactions have been carried out
with a homogeneous catalyst entrapped in the
aqueous domain and the substrate present in the
continuous nonpolar domain of either a
microemulsion or a suspension of mesoporous
material. Both metal-organic complexes and
enzymes have been used as heterogeneous
catalyst. The efficiency has generally been good
and the activity of the catalysts, including
enzymes, has been high.55
54
KH Eur J Org. Chem 5 (2007) 731
KH Adv. Colloid Interface Sci. 51 (1994), Alami, KH J.
Colloid Interface Sci. 239 (2001) 230, Härelind, KH et al
55
17
Karin Markides 511124-1021
Supra Centre Chalmers
Feruloyl esterases
Feruloyl esterase is a minor enzyme activity in the
enzyme battery that many filamentous fungi
produce. Even though not produced commercially,
it has a great potential for biosynthetic reactions as
well as for degradation of cross-linking in
lignocellulosic material. We have identified a large
number of filamentous fungi strains that are able to
produce feruloyl esterases. Furthermore, we have
investigated their substrate specificity and have
shown that there is a large diversity in which
bonds they are able to hydrolyse.
Transport in confined media
Electrophoretic and diffusional transport have
been studied in nano-porous polymeric gels, liquid
crystals (micellar and bicontinuous cubic phases)
and lipid nanotubes that connect two liposomes. In
the gels we have developed a method to purify
circular plasmids by topological capture.56 In
liquid crystals the well-defined structure allowed
systematic studies of size, flexibility and topology
of the analyte, and the interaction between analyte
and the pore wall. Imperfections in the liquid
crystals could also be studied, because DNAmolecules tend to move along grain boundaries in
the polycrystalline crystals.57 In the lipid
nanotube-vesicle systems we demonstrated singlefile counting of both DNA and particles.58
Cell factories
We have a long experience in developing cell
factories for industrial biotechnology, e.g for
production of ethanol and succinate. With the aim
of designing more efficient cell factories suitable
for industrial conditions, quantitative systems
biology tools has been developed that can combine
Appendix A
high-throughput data with mathematical modeling
to aid identification of new metabolic engineering
targets.59 Based on fermentation model systems
that mimic industrial process conditions new
targets, such as intolerance to various
meatabolites, has been indentified as limiting in
the cell factory development.60 Such observations
call for an increased molecular understanding of
events at the cellular membrane.
Metal nanoclusters - designed optical properties
and interactions with biomolecules
We have developed a unique understanding of
how resonance energy transfer between selfassembled silver or gold nano-clusters is affected
by surface plasmon states, and have also studied
how such metal particles interact with DNA,
proteins and lipids. By using electron beam
lithography to control the interparticle distances in
particle arrays, we have reached a profound
knowledge on how long-range and short range
interactions affect the optical properties of
nanostructures.61 These fundamental and applied
studies have been extended to other metals like Pt
and Pd.
Delineating the mechanisms behind single
molecule SERS
SERS - Surface-enhanced Raman scattering - has
been know since the late 70's, but the field took an
unexpected turn in 1997, when two groups
independently reported that SERS could be used to
obtain vibrational Raman spectra from single
molecules. This was a staggering claim, implying
an enhancement factor of ten orders of magnitude!
In a series of papers62 which now have
59
Langmuir 18 (2002) 1811, KH J. Colloid Interface Sci.
274 (2004) 355, Häger, KH Chem. Eur. J. 10 (2004) 5460.
56
BÅ JACS 121 (1999) 7292; Cole, BÅ et al
Electrophoresis 27 (2006) 4396
57
Svingen, BÅ J. Phys. Chem. B 108 (2004) 2735,
Carlsson, BÅ et al J. Phys. Chem. B109 (2005) 18268;
Langmuir 22 (2006) 4408,
58
Tokarz, BÅ, OO, et al PNAS 102 (2005) 9127, Langmuir
23 (2007) 7652
Otero, LO et al Adv Biochem Engin/Biotechnol 108
(207) 1; Devantier, LO et al, Ind Biotechnol 1 (2005) 51
60
Zaldivar, LO et al Appl. Microbiol Biotechnol 56 (2001) 17
61
Svedberg, MK et al Nano Lett 6 (2006) 2639; LG, MK et
al J Phys Chem B 107 (2003) 7337; Hicks, LG, MK et al
Nano Letters 5 (2005) 1065; LG, MK et al Appl. Phys. Lett.,
78 (2001) 802; Haynes, LG, MK et al J. Phys. Chem. B 107
(2003) 7337
62
Xu, MK et al Phys Rev Lett 83 (1999) 4357; Xu, MK et al
Phys Rev E 62 (2000) 4318; Bjerneld, MK et al Single Mol 1
18
Karin Markides 511124-1021
Supra Centre Chalmers
accumulated ~700 citations, we investigated this
phenomenon experimentally and theoretically.
Using generalized Mie theory, we showed
that the enormous SERS amplification could be
understood if it was assumed that the single
molecule was situated at a "hot site" formed in the
crevice
between
two
aggregated
metal
nanoparticles, which acted as "nanoantennas" in
the Raman scattering process. We then tested this
hypothesis by self-assembling pairs of colloidal
silver particles using single heme protein
molecules, acting as the "glue" between the
particles. These nano-constructs exhibited all the
expected characteristics of single molecule SERS,
including
fluctuating
heme-vibrations,
characteristic of the single molecule limit, and the
enhancement factor (~1010) and polarization
dependence was found to be in excellent agreement
with our theory.
Appendix A
monitoring technique,64 which was developed by
the group in the 90's and commercialized by QSense Inc (www.q-sense.com).65 More recently,
the group combined the QCM-D technique with
surface plasmon resonance (SPR)66, including the
extension of the latter principle to nano-scale
formats.
Assembly and fusion in liposome-systems
We are internationally recognized in the field of
supported membrane assembly, including their
integration with nanoplasmonic sensor formats.67
The group also pioneered the methodology of
DNA-directed immobilization as a means for the
self-assembly of lipid vesicle arrays, later refined
and extended to programmed membrane fusion
that mimic protein-based agents by using synthetic
oligonucleotides68
Controlled Energy and Electron Transfer Rates
We have studied the photoinduced transfer
reactions: singlet energy transfer (SET), triplet
energy transfer (TET), and electron transfer (ET)
in sets of closely related model systems.
The systems are based on porphyrin donors
and acceptors covalently connected by bridging
chromophores either with varying electronic
structure or length. By varying the electronic
structure of the bridging molecule the barrier
height for the tunneling electron or exciton is
changed systematically and by varying the length
of the bridge molecule the corresponding barrier
width is changed.
Theoretical calculation of the optical trapping
potential in the crevice between two silverparticles
in water.63
Biosensing by mass, viscosity and plasmons
We have expertise in affinity-based bioanalytical
sensors, in particular the quartz crystal
microbalance
with
dissipation
(QCM-D)
(2000) 239, Bjerneld, MK et al J Phys Chem B 106 (2002)
1213; Xu, MK Chem Phys Chem 4 (2003) 1001.
63
Xu, MK Phys Rev Lett 89 (2002) 246802
64
FH, Breszinski et al. PNAS. 95 (1998) 12271; FH, Elwing
et al Anal. Chem. 73 (2001) 5796
65
Rodahl, FH et al Rev. Sci. Instrum. 66 (1995) 3924
66
Reimhult, FH et al Anal. Chem. 76 (2004) 7211; Dahlin,
MK, FH et al JACS 127 (2005) 5043.
67
Reimhult, FH, Kasemo. Langmuir 19, (2003) 1681,
Jonsson, FH et al. Nano Letters (2007), in press.
68
SS, FH et al ChemBioChem 4 (2003) 339 ; Pfeiffer, FH
JACS 126 (2004) 10224; Stengel, FH et al JACS 129 (2007)
9584; FH, BN et al Langmuir 17 (2001) 8305; AS, FH, ,
AEw, OO et al Anal Chem, 73 (2001) 5805
19
Karin Markides 511124-1021
Supra Centre Chalmers
SET, TET, ET
N
N
M
N
R
N
N
N
M = Zn, 2H
m
N
N
m = 2H, Fe(III), Au(III)
R
OB
Barrier height
BB
NB
AB
Barrier width
n = 0, 1, 2, 3
2B-5B
Model systems for the study of electron and excitation
energy transfer.
From photophysical studies and quantum
mechanical DFT calculations of these carefully
designed model systems, we have been able to
develop a quantitative understanding of the factors
that dictate the distance and energy dependence of
the electronic coupling or equivalently the transfer
rates.69 This knowledge together with the extensive
literature on the driving force/reorganization
energy-matching problem makes it, for the first
time, possible to a priori design molecular systems
with predictable electron transfer properties. We
believe that this systematic approach, in contrast to
a trial and error search, is necessary for efficient
development of both photovoltaic materials and
molecular electronic components. In fact, a
number of recently started projects aiming for
improving dye-sensitized solar cells and materials
for molecular electronic applications (wires and
switches) have taken inspiration from this
knowledge.70
Appendix A
closely related to the electron transfer problem that
we early addressed71 and have pursued since, as
more structural data of photosystems have become
available. The photo-excited electron is funneled
into the RC via a pair of bacteriochlorophyll
(BChl) molecules, then passes on to an
"accessory" BChl, until it finally drives a sequence
of proton transfer reactions. Understanding details
of this process is important in the context of design
of other molecular electron transfer systems,
including conducting polymers, light-emitting
diodes and photo-voltaic systems.
Synthesis of semiconducting polymers
We have synthesized conjugated polymers for
different types of electronic and photonic devices.
Already in 1994 we managed to develop polymers
which could be used to prepare light-emitting
diodes with variable colors. The light emitted by
the polymers covered the entire visible spectrum.
By using blends of different polymers, voltage
controlled emission could be shown. Our polymers
have also been used for making solar cells by
lamination techniques. The polymer exhibited
extended absorption compared to other materials at
that time.72 A new class of conjugated polymers
(low bandgap alternating polyflurene copolymers)
was synthesized for solar cells. They have turned
out to be a very promising type of polymers for
this application. More recent polymers of this class
have extended the absorption up to 1000 nm in
solar cells, which we were the first to achieve.73
Theoretical studies of electron transfer
mechanisms in biological systems
With the crystal structure of a photosynthetic
reaction center (RC) theoretical studies to understand the mechanisms of light harvesting and
charge separation became meaningful. The latter is
69
Kilså-Jensen, JM, BA JACS 123 (2001) 3069; Pettersson,
JM, BA J. Phys Chem A 110 (2006) 319; BA et al Phys.
Chem. Chem. Phys. DOI:10.1039/B706122F, in press 2007
70
Winters, BA et al JACS 129 (2007) 4291; Andréasson, BA,
Gust et al JACS 128 (2006) 16259
71
SL JACS 103 (1981) 4034
Berggren, MA, Wennerström et al Nature 372 (1994) 444;
Granström, MA, Friend et al Nature 395 (1998) 257
73
Svensson, MA et al Advanced Materials 15 (2003) 988;
Wang, MA, Inganäs Applied Physics Letters 85 (2004) 5081
72
20
Kod
2007-14950-54750-89
Name of applicant
Markides, Karin
Date of birth
511124-1021
Title of research programme
Centre for Bio-inspired Supramolecular Function and Design
Appendix
B
Curriculum vitae
VRAPS/VR-Direct bilaga 2004.Be
Vetenskapsrådet, SE-103 78 Stockholm, tel. +46 (0)8 546 44 000, [email protected]
Karin Markides 511124-1021
Supra Centre Chalmers
Bengt Nordén
Appendix B
Curriculum Vitae
PhD 1971 and "Docent" 1972
Education: University of Lund MSc 1968 in Mathematics, Theoretical Physics and (Major) Chemistry, PhD
1971 (Polarized Spectroscopy and Electronic Transition Moments). "Oavlönad docent" 1972-01.
Present position: The Chair of Physical Chemistry of Chalmers University of Technology, Gothenburg
(appointment by the Swedish Government 1979 - ).
Previous employments etc: Position as Associate Prof. (Docent) of Inorganic Chemistry University of Lund
1972-78; Acting Chair of Inorganic Chemistry, University of Lund 1978. Leave of absence for military
service during 1972 (as Chemical & Nuclear Defence Officer). Sabbatical leaves: Visiting Professor of
Oregon Univ. Eugene (in 1978 c/o Schellman), Copenhagen Univ. (1990 c/o Buchardt) and at California
Institute of Technology (2004 c/o Zewail).
Awards etc
Fabian Gyllenborg’s Prize for PhD thesis in Chemistry, Lund 1972 (the first time the prize was awarded);
The Göran Gustafsson’s Prize for Chemistry 1992 (the second time this prize was awarded); Honor. Fell.
Australian National University 1996; The Swedish Svante Arrhenius Medal 1994. Honor. Professor of Univ.
of Sechuan 2006. Member elect of Academies: the Royal Swedish Academy of Sciences, the Royal Swedish
Academy of Engineering Sciences, the Royal Physiographic Society (Lund), the Royal Society of Arts and
Sciences (Gothenburg), the European Academy (Academia Europeae), and the German Academy of
Sciences (Leopoldina), the Swedish Academy of Engineering Sciences in Finland and the Norwegian
Academy of Sciences. Ten honorary lectures, incl. The Guggenheim Memorial Lecture of 1993 (Reading
Univ.), The William Chalmers Lecture of 1998 and The Kroc Lecture of 2005 (M.I.T.).
Number of students brought to PhD (with BN main supervisor): 31 (7 women), of whom three
participate in the Supra Centre Project, now professors: Bo Albinsson, Per Lincoln and Björn Åkerman.
Number of postdocs/visiting professors (with BN as advisor/host): 18 (3 women)
Jonas (1976-78), Matsuoka (1980-83), Sluydkin (1982-83), Ovaska (1984), Rodger (1986), Tokay (198486), Fragata (1975-86), Takahashi (1986-87), Kim (1987-89), Tuite (1994-1999), Vickery (1997-98), Ray
(1999-2001), Morimatsu (2002-05). Schipper, Tokay, Fragata, Takahashi later also as Visiting Professors.
Profs Schellman and Kurucsev spent sabbatical years with me as their host in 1985-1987.
Publications: author of some 360 original scientific papers, 10 review articles and one textbook.
National & International Commissions of Trust. President of Class IV of the Royal Swedish Academy of
Sciences (awarding the Nobel Prize in Chemistry) 2004-2009 and Member of the Governing Board of the
Academy 2005-. Prize Committee of the Millenium Technology Prize (Finland) 2006-. Board of Directors
of Hasselblad's Foundation of Photography and Scientific Research. Chairman of Intl. Evaluation of the
Chemistry Institutions of the University of Lund 2007. Director of EU program ”Addressable Molecular
Nucleic-acid-based Assembly". Editorial Board of the Journal of Physical Chemistry, Chemical Physics
Letters, Biophysica et Biochimica Acta. Editor in Chief of Quarterly Reviews of Biophysics. Chairman of
CERC3, the Council of the European Research Councils’ Chemistry Committees 2006-2007. Chairman of
the Board of Directors of the US Molecular Frontiers Foundation (MFF), a global outreach program, and of
the Swedish MFF of the Royal Swedish Academy of Sciences. For contribution by B.N. and impact, see
Joanne Kotz: Nature Chemical Biology 3, 79 (2007) doi:10.1038/nchembio 0207-79.
Previous commitments. Chairman of the Nobel Committee for Chemistry 2001-2003. Counselor of the
Swedish Science Minister (1992-94), The Swedish Research Council (NFR-VR): Chairman of its Chemistry
Section 1998-2003. CEO of "Strategic Nucleic Acids" of Swedish Foundation for Strategic Research.
Chairman of International Evaluations: Theoretical Chemistry in Sweden (1988), Synchrotron Research in
Sweden (1990), Chemistry at: University of Lund (1989), University of Copenhagen (2004), University of
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Appendix B
Karlstad (2005). Director of EU Biomedical Gene Targeting program (1995-2000). Expert reviewer at some
40 appointments to Chairs in chemical sciences in Sweden and abroad. Chair of the Final Selection
Committee of the European Young Scientists’ Awards (EURYI) of the European Science Foundation (20042006).
Research sponsored by Swedish Research Council (VR), Swedish Cancer Foundation, Foundation for
Strategic Research (SSF), European Commission (EU). Ca 7 MSEK/year.
Scientific interests. Mechanisms of molecular recognition, specifically for nucleic acids. DNAsupramolecular structures with chiral substitution-inert metal complexes and peptide nucleic acids.
Spectroscopy with polarized light (flow dichroism) addressing biomolecular structure, e.g. 3D structure of
DNA-recombinase complex in solution. Membrane translocation mechanisms of cell-penetrating pepides.
Major research achievements
Development and study DNA-binding ligands, including bis-intercalating ruthenium compounds and peptide
nucleic acids (PNA). Optical spectroscopic techniques developed to this end have provided detailed
information about binding geometries and interaction mechanisms at an atomic level determining recognition
properties. A remarkable reorganisation process has recently been discovered between groove-binding and
intercalation binding geometries requiring a coherent opening of adjacent base-pairs, as well a discovery of
micelle-catalysed dissociation of DNA complexes. Several cases of molecular recognition due to kinetic
(instead of thermodynamic) selection have been demonstrated. A major achievement was a solution-structure
determination based on systematic molecular replacement of aromatic aminoacids in a RecA-DNA complex
revealing details of the recombination mechanism.
International evaluations
In the international evaluation of Biotechnology in Sweden, conducted by the Swedish Research Council in
2003, the research was classified as 'outstanding'.
Research results of benefit for research, technology and society
Inventions and development of:
Peptide nucleic acids (PNA), gene-diagnostic, therapeutic and supramolecular uses (2 US patents).
DNA probes based on modified fluorescent nucleobase analogs and ruthenium(II) polypyridyl compounds
with applications in gene-targeting and cell-staining.
Binuclear ruthenium compounds as leads for cancer-therapeutic drugs (1 patent).
Polarized-light spectrocopy (linear dichroism) methods to study nucleic-acid-ligand structures, including
discovery of general ”ambivalent” groove and intercalative binding.
Site-Selected Linear Dichroism by molecular replacement (SSLD): method for protein-structure
determination in solution for fibrous complexes, not amenable to x-ray or NMR study, based on
chromophore replacements.
Outreach & Popular Science Activities
Chemistry expert and coeditor of NE, the Swedish National Encyclopedia 1989- (and author of 98 articles in
NE). Founder of Molecular Frontiers (MF), a global organization with objective to stimulate young people'
interests in science by entry through the world of molecules. Chairman of the American Molecular Frontiers
Foundation, and also of the Swedish sister MF foundation hosted by the Royal Swedish Academy of
Sciences. The Scientific Advisory Board of MF includes 25 eminent chemists, physicists and mathematicians
(including 8 yet very research active Chemistry Nobel Laureates) - see www.molecularfrontiers.org and
www.moleclues.org.
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Appendix B
Curriculum Vitae
Bo Johnny Albinsson, born 1963-02-01 in Göteborg, Sweden.
Department of Chemical and Biological Engineering/Physical Chemistry, Chalmers University
of Technology, 412 96 Göteborg, Sweden. Phone: +46-31-772 3044, Fax: +46-31-772 3858.
[email protected].
Education and degrees
Master of Science in chemical engineering, Chalmers University of Technology
•
1987-06-30.
PhD in Physical Chemistry at Chalmers University of Technology 1993-03-15,
•
supervisor: Bengt Nordén.
Docent in Physical Chemistry at Chalmers University of Technology 1997-10-21
•
Academic positions
•
•
•
•
•
•
•
•
•
Post-doc with prof. Josef Michl, University of Colorado at Boulder, 1993-1995
Assistant professor (Forskarassistent) in Physical Chemistry at Chalmers
University of Technology 1995-1999
Associate professor (docenttjänst) in Physical Chemistry at Chalmers University
of Technology 1999-2001
Professor (bitr. professor) in Physical Chemistry at Chalmers University of
Technology 2001-.
Professor (professor) in Physical Chemistry at Chalmers University of
Technology 2003-.
Appointed director of studies (studierektor) for the school of Chemical
Engineering Physics (Kf) at Chalmers University of Technology, 1998-2002.
Appointed director of studies for the graduate school in chemistry at Chalmers
University of Technology, 2001-2002.
Appointed vice head of the Department of Physical Chemistry, Chalmers 20012002.
Appointed head of the Department of Chemistry and Bioscience, Chalmers 2002-
Academic scholarships and appointments
Awarded scholarship for doctoral studies at Chalmers University of Technology,
•
1987-1993.
Awarded
scholarship from NFR for post doctoral work at University of Colorado,
•
Boulder 1993-1995.
Appointed Research Associate (Forskarassistent) by NFR at the Department of
•
Physical Chemistry, Chalmers University of Technology. 1995-1999
Awarded
"Junior Individual Grant" from the Chalmers foundation 1997-09-01.
•
Appointed Senior Researcher (särskild forskare) by NFR in the subject
•
“Molecular aspects on energy conversion in biological and biomimetic systems,
1999-07-01 – 2005-06-30.
Ph. D exams, supervising experience
Kristine Kilså Jensen, Ph. D 2000. Now Associate professor at University of
•
Copenhagen; Joakim Andréasson, Ph. D 2002. Now Assistant professor at
Chalmers University of Technology; Mikael Winters, Ph. D 2007; Karin
Pettersson, Ph. D 2007; Mattias Eng, Ph. D 2007.
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Karin Markides 511124-1021
•
•
•
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Appendix B
Co-supervised Drs. Daniel Nilsson (2007), Thitinun Monhapol (2007), Anders
Holmén (1999), Anette Larsson (1995), Christina Carlsson (1997) and HansChristian Becker (2000).
Presently supervising graduate students: Karl Börjesson, Joakim Kärnbratt,
Joanna Wiberg (Lic. 2007), Peter Sandin (Lic 2005) and Jonas Hannestad.
Post-docs Alexander Kyrychenko, Ukraine 2000-2002, Liqian Li, China 2003
Aldo Jesorka, Germany 2004, Marcus Wilhelmsson, Sweden 2004, and Lijun
Guo, China 2005.
Committees and Editorial work
I participated in the evaluation of research proposals to the Swedish research
•
council (VR) during 2000 (NFR, chemistry) and 2001-2003 (VR, theoretical and
physical chemistry)
I was appointed to act as chairman for the theoretical and physical chemistry
•
group of VR for the periods 2004-2006 and 2007-2009
I have been the external examiner (opponent) for Mikael Isaksson (Umeå Univ.,
•
2007), Niclas Sandström (Uppsala Univ., 2007), James Hutchison (Univ. of
Melbourne, 2006), Bo W. Laursen (Univ. of Copenhagen, 2001) and James
Rostron (Univ. of Newcastle 2005).
I was a member of the International Scientific Committee arranging the 17th
•
IUPAC Symposium on Photochemistry in Barcelona 1998.
I act as a referee for several major scientific journals (Journal of the American
•
Chemical Society, Angewandte Chemie, Chemistry, A Eur. Journal….).
I acted as evaluator for the Nobel Committee in Chemistry.
•
Appointed member of the faculty senate (fakultetsrådet) at Chalmers University of
•
Technology, 2003-2008.
Appointed Chairman for the 22nd IUPAC Symposium on Photochemistry to be
•
held in Göteborg, 2008. This conference is the biggest in the photochemistry field
and usually attracts some 600 delegates.
Swedish
representative in the domain committee for chemistry, molecular sciences
•
and technology (DC CMST) in the COST programme run by the European
Science Foundation 2006-2008.
Present and recent funding (to BA as main applicant or BAs share, in units of 1000 kr)
Granting agent
•
•
Years
Funds per
year
1217
400
Funds
total
3651
2000
VR
2006-2008
Material Science, research
2002-2006
school
VR, laser equipment
2003
3200
3200
•
VR, research position
1999-2005
Ca 1000
6000
•
a
EU,
STREP
–
AMNA
(my
2005-2007
800
2400
•
share)
SSF – Nano-X (my share)
2006-2010
600
3000b
•
KAW – solar cells and solar
2006-2010
700
3350c
•
fuels (my share)
a
Total grant of 2 500 k€. bTotal grant of 14 000 kkr. cTotal grant of 46 000 kkr.
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Appendix B
Curriculum Vitae
2007-10-26
Name: Mats R. Andersson
Born: May 27, 1966
Marital status: Married, two children
Address: Genvägen 4, SE-449 51 Alafors, Sweden
Telephone: 0303-740844, 0705-340844
Present position: Professor (Sv. Biträdande Professor) in Polymer Chemistry
Department of Chemical and Biological Engineering / Polymer Technology
Chalmers University of Technology
SE-412 96 Göteborg, Sweden
Tel. 031-772 3401, Fax 031-772 3418
E-mail: [email protected]
Homepage www.chalmers.se/chem/EN/divisions/polymertechnology
1. PhD, "Synthesis and properties of substituted poly(thiophenes)", Department of Organic
Chemistry, Chalmers University of Technology, March 24, 1995.
2. Post-doc., Institute for Polymers and Organic Solids, University of California at Santa
Barbara, in Prof. Alan J. Heegers group, 1995-1996, 1 year.
3. Docent, Department of Polymer Technology, Chalmers University of Technology,
October 12, 2000.
4. Professor (Sv. Biträdande Professor) in Polymer Chemistry, July 1 2004 Department of
Chemical and Biological Engineering / Polymer Technology, Chalmers University of
Technology, presently financed by a Senior researcher position, VR, 70% research
5. Previous positions:
• Associate professor (docenttjänst) May 1 2001, permanent position, Department of
Polymer Technology, Chalmers University of Technology.
• Lecturer (universitetslektor) April 1 2001, Department of Polymer Technology,
Chalmers University of Technology.
• Junior researcher (forskarassistent) April 1 1997, Department of Polymer
Technology, Chalmers University of Technology.
• Temporary Junior researcher (vikarierande forskarassistent) January 1, 1997,
Department of Polymer Technology, Chalmers University of Technology.
• Researcher from May 1 1996, Department of Polymer Technology, Chalmers
University of Technology.
• Post-doc, April 10 1995, Institute for Polymers and Organic Solids, University of
California at Santa Barbara, financed by TFR.
• PhD-student, started October 1 1990, Department of Organic Chemistry, Chalmers
University of Technology. 6 Months of the PhD-studies was done at University of
California, Santa Barbara in Prof. Alan J. Heegers group, 1991, supported by
”Sverige-Amerikastiftelsen”.
6. Parental leave: 50%, 00-09-01 - 00-12-31 and 50%, 02-09-01 - 02-12-16
7. Supervisor for PhD students:
• Mikael Johansson, Department of Organic Chemistry, main supervisor, PhD 2001.
• Maria Jonforsen, Department of Polymer Technology, main supervisor, PhD 2002.
• Mattias Svensson, Department of Organic Chemistry, main supervisor, PhD 2003.
• Erik Perzon, Department of Materials and Surface Chemistry / Polymer Technology, main
supervisor, PhD 2007.
• Jonas Engström, Department of Materials and Surface Chemistry / Polymer
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Appendix B
Technology, co supervisor, PhD 2006.
• Lars Lindgren, Department of Materials and Surface Chemistry / Polymer
Technology, main supervisor, started 2003.
• Stefan Hellström, Department of Materials and Surface Chemistry / Polymer
Technology, main supervisor, started 2005.
• Villgot Englund, Department of Materials and Surface Chemistry / Polymer
Technology, co supervisor, started 2004.
Supervisor for Post Docs / Guest researchers:
• Wendimagegn Mammo, Department of Organic Chemistry, main supervisor, 1
year, 1996-1997, 3 months 1998 and 2003, and 1 year 2004-2005.
• Dolores Caras-Quintero (post doc), Polymer Technology, main supervisor, 3
months, 2004.
University commitments:
• Deputy head of Polymer Technology (stf prefekt), 1998 - 2002
• Responsible for “kompetensinriktning Material”, K4, Chalmers, 1998 -2007
• Coordinator in the “Graduate School of Materials Science”, Chalmers, 1997 –2006
• Representing “Forskarskolan i materialvetenskap, K” in “SSK:s kommitté för
forsknings- och forskarutbildningsfrågor”, 1998-1999
• Representing teachers in “kommittén för grundutbildningsfrågor K”, 2001 -2005
• Deputy head and economical responsible for 2160 (Applied Chemistry), 2007Other qualifications:
• Member of the evaluation committee for Materials Science, NT-Q, Swedish
Research Council, 2006 and 2007
• Participated in “Ledningsutvecklingsprogram för yngre forskningsledare”,
Chalmers 2006-2007
• Expert opinion to the Nobel Committee in chemistry, 2005
• Opponent for Björn Atthoff, Uppsala University, 2006, Main supervisor Jöns
Hillborn
• Member of the examination committee for more than 7 PhD students
Invited talks:
• Seminar, Cambridge, England, May 17, 2005, “Low band-gap polyfluorenes for
solar cells”
• Conference, Strasbourg, E-MRS, May 29, 2007, “Design, Synthesis, and Devices
of Low Band Gap Conjugated Polymers”
Publications: A complete list of refereed papers includes 144 references, see publication list.
Patent: Colour source and method for its fabrication, WO9531515, now sold to Cambridge
Display Technology. Bengt Göran Gustafsson, Olle Werner Inganäs, Ulf Thomas Hjertberg, Rolf
Magnus Berggren, Mats Roland Andersson.
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Appendix B
Curriculum Vitae, Linda Gunnarsson
Date of birth:
Gender:
December 29, 1971
Female
ACADEMIC DEGREES
PhD in Physics
2004-01-20
Optical properties of silver nanoparticles prepared by electron beam lithography
Licenciate of Engineer (Tekn. Lic.)
1999-06-10
MSc Engineering Physics, Umeå University (Civ.Ing.)
1996-09-01
EMPLOYMENTS
Assistant Professor Jan 2006- current
Department of Applied Physics, Chalmers University of technology,
Research assistant Jun. 2005-Dec. 2005, Oct 2004-Jan 2005, Feb 2004-Jun 2004
Department of Applied Physics, Chalmers University of technology
Postgraduate Sept. 1996-Jan. 2004
Department of Applied Physics, Chalmers University of Technology
EDUCATION
Pedagogical Education-senior high school teacher (Allmänt Utbildningsområde, 60p)
Göteborg University
sept 2005- ongoing.
Research school in physics
1996-2004
Chalmers University of Technology
Engineering Physics
1991-1996
Umeå University
Senior High School
1988-1990
Teknisk linje, maskinteknisk inriktning, Wargentinskolan, Östersund
PERIODS OF PARENTAL LEAVE + LEAVE FOR STUDIES
1999 – 2000 and 2002 – 2004, 2006-2007 Total of ~3 year full time parental leave
2006 01 01-2006 05 30 Leave for studies (tjänstledig pga av studier)
SCIENTIFIC INTEREST
The physics of localized surface plasmons (LSP’s) in nanoscale metal structures and their
dependence on size, shape and electromagnetic interactions. Applications of LSP’s for surface
enhanced Raman scattering (SERS), biosensing, bioimaging and photothermal treatment of
tumours. Expert in nanofabrication with emphasis on electron beam lithography.
RESEARCH EXPERIENCE
• Systematically examined the optical properties of silver particles with different shapes, sizes
and arrangement prepared by electron-beam lithography. The optical properties were
characterized with Raman spectroscopy (using different Raman active probe molecules
attached to the particles), UV-Vis-NIR and darkfield spectroscopy
• Fabricated and characterized single-electron tunneling transistors based on a combination of
chemical synthesis of gold clusters, self-assembly of thiols and electron-beam lithography.
This principle is the base for an awarded patent on a biosensor and a newly started company.
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Karin Markides 511124-1021
•
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Appendix B
Long experience and high competence in micro- and nanofabrication primarily utilizing
lithography (electron-beam, photo and colloidal), etching and chemical and physical vapour
deposition.
RESEARCH SPONSORED BY:
Swedish research council (VR)- 2006, 4 year Assistant Professor position.
EU FP6 Network of Excellence for Plasmo-Nano-Devices – 2007, Financing a 1 year post doc in
biosensing development.
OTHER MERITS
• Ongoing studies in pedagogics for senior high school teaching (only a 10 week diploma
thesis left).
• Involved with a project at Chalmers School of Entrepreneurs to develop a commersiable
biosensor.
• Giving a public oriented talk at an open seminar arranged by the Swedish research
council November 2005. “Nanopartiklar för cancerdiagnostik “ (Nanoparticles for
cancer diagnostics)
• June 2005: Public oriented lectures about Nanotechnology for college students at Lerums
Gymnasieskola.
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Appendix B
CV for Krister Holmberg
Doctoral degree
Ph D (Tekn dr) in Organic chemistry from Chalmers University of Technology
1974
Qualification as Associate Professor
Docent in Organic Chemistry at Chalmers University of Technology 1984
Current position, period of appointment, time for research in the position
Professor in Applied Surface Chemistry since 1998
Head of Department of Chemical and Biological Engineering since 2003
No specified time for research
Previous positions and periods of appointment
1974 - 1976
Research Chemist, AB Leo, Helsingborg
1976 - 1979
Research Manager, SOAB AB, Mölndal
1979 - 1983
Research Manager, Eka Kemi AB, Bohus
1983 - 1988
Assistant Director of Research, Berol Nobel AB, Stenungsund
1984 - 1991 Adjunct Professor of Biotechnological Surface Chemistry,
Göteborg University
1988 - 1991
1991 - 1998
Director of Research, Berol Nobel AB, Stenungsund
Managing Director of the Institute for Surface Chemistry,
Stockholm
1992 - 1998 Adjunct Professor of Surface Chemistry, Royal Institute of
Technology, Stockholm
1997 (spring)
Visiting Professor, Université P. et M. Curie, Paris
People awarded doctorates for whom I have been the main supervisor
Karin Bergström, Carina Brink, Peter Skagerlind, Britta Folmer, Norman Burns,
El Ouafi Alami, Maria Häger, Per Kjellin, Fredrik Currie, Maria Stjerndahl, Dan
Lundberg, Kristina Mohlin, Maj-Britt Stark (licentiate), Eva Österberg (licentiate),
Per-Erik Hellberg (licentiate), Paul Handa, Tomasz Witula
Postdoctoral researchers who are or have been engaged in collaboration with me in the research
group
Seong-Geon Oh, Brajesh Kumar Jha, Anil Kumar, Stan Lam, Victor Seredyuk,
Neil Cruse, Jean-Christophe Beziat, Lyuba Shtykova, Camilla Fant, Joseph Bauer,
Martin Andersson, Romain Barres
Distinctions
Member of the Swedish Royal Academy of Engineering Sciences 1990 -;
Chairman of the Chemistry Division 2002-2004.
President of the Swedish Chemical Society 1999-2005
Member of the Swedish National Committee for Chemistry 2000 Fellow of the Royal Society of Chemistry 1993President of the International Association of Colloid and Interface Scientists 1996 1998
l'Ordre National du Mérite au grade de Chevalier 2000
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Appendix B
Member of the Royal Society of Arts and Sciences in Göteborg 2004
The Oscar Carlson Award 2006
Fellow of the European Academy of Sciences 2007
National and international assignments of importance
Chairman of Chemical Engineering at the Swedish Research Council 2002-2003
Member of the Scientific Board of NUTEK 1992-1997
Member of the Strategy Group for Chemical Engineering at the Foundation for
Strategic Research (SSF) 1996-2000
Member of the Strategy Group for Life Sciences at the Foundation for Strategic
Research (SSF) 2000-2004
Member of the Expert Group for Research at the KK Foundation 2004Program Director for Marine Paints, financed by the Foundation for Strategic
Environmental Research (MISTRA) 2003
Director of Studies of the Research School for Colloid and Interface Technology
financed by SSF 1998-2005
Program Director of the Research School YPK (Ytkemi för plast- och
kemiindustrin) financed by the KK Foundation and 12 companies 2005Swedish representative in the Management Committee of COST D19
(Nanochemistry) 2002-2006
Member of the Advisory Group of the IUPAP Physical and Biophysical Chemistry
Division 2001 Editor of Current Opinion in Colloid and Interface Science (Elsevier)
Member of the Editorial Board of Journal of Surfactants and Detergents
Member of the Editorial Board of Annual Surfactants Review
Member of the Editorial Board of Journal of Dispersion Science and Technology
Member of the Ownership Board of Physical Chemistry Chemical Physics
Member of the Board of Tryckteknisk forskning AB
Member of the Scientific Board of Oxiteno SA
Member of the Scientific Board of Appeartex AB
Publications, etc
I have published or submitted 213 scientific papers, including peer reviewed
conference proceedings. 66 of these are from the period 2003-2007. I am author or
editor of six books and several compendiums.
I am the inventor or co-inventor of 36 patents or patent applications, assigned to the
following companies: AB Leo, Eka AB/Eka Chemicals AB, Berol Kemi AB/Berol
Nobel AB, Camurus AB, Appeartex AB, and I-Tech AB.
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Appendix B
CV for Fredrik Höök
Div. of Biological Physics; Dept of Applied Physics, Chalmers University of Technology; Fysikgränd 3, SE412 96 Göteborg, Sweden; Phone: +46-31-7726109; E-mail: [email protected], Web-page:
http://chalmers.fy.se
1) PhD
1997 (Dept of Appl Phys, Chalmers Univ. of Tech., Göteborg, Sweden)
2) Postdoctoral position:
1997-99 (Dept of Cell and Molecular Biology Göteborg Univ.)
3) Qualification as associated Professor: 2004
4) Present position:
2007
Professor of Biological Physics, Chalmers University of Technology (80%)
2006-:
Rådsforskare in proteomics from
2004- 2007 Professor of Nanoscience for Biophysics, Lund University (20%)
5) Former positions:
2000-2004: Assist. Prof of Biological Physics, Dept of Appl. Phys., Chalmers
1999-2000: Assist. Prof. of Surface Biotech., Dept. of Cell and Molecular Biology, GU
1998-1999: Doctoral position, Dept. of Cell and Molecular Biology, GU/Dept of Applied Phys. CTH
1992-1997 PhD position
6) Parental leave, military service etc
Parental leave: six months 1998, three months 2005.
7) PhD students/postdocs under my supervision1
Main supervisor for (4): Annette Granéli (2003), Charlotte Larsson (2005), Indriati Pfeiffer (2006), Malin
Edvardsson (2006).
Co-supervisor for (2): Linda Olofsson (2003) and Erik Reimhult (2003)
Currently main supervisor/examiner for (7): Andreas Dahlin (2004-), Magnus Jonsson (2005-), Jason Beech
(2006-), Peter Jönsson (2006-), Jakob Malm (2006-), Lisa Simonsson (2007-), Seyed Tabaei (2007-)
Co-supervisor for (2): Jacob Vallkil (2006-) at Immunotechnology, Lund Univ., Nina Tymchenko (2007-),
Chalmers
Previous Postdocs (5): Sofia Svedhem (2003-2004), presently at Appl Physics, Chalmers, Gouliang Wang
(2003-2004) Presently at Q-Sense AB, Göteborg, Jason Benkoski (2003-2005) presently at NIST, US,
Rodolphe Marie (2005-2006), Lund Univ., presently on paternally leave, Christelle Prinz, (2005-2007),
presently on paternally leave
Current Postdocs (2): Gudrun Stengel (2006-), Previously at Scripps Inst, La Jolla, US), Ye Shou, (2005-)
shared with B. Liedberg at LiU
Examiner for >20 diploma students.
8) National and international commissions
I. Member of evaluation committees for >15 PhD-thesis defenses within Sweden, and have been PhD
opponent six times (3 in Sweden):
II. Invited conference contributions
Invited speaker at ~3 international conferences/meetings annually 2001-2004, and >5 annually
1
I will remain responsible for the listed PhD students, who will all join my new activities at Chalmers. At Chalmers,
I will also take over the responsibility for two new PhD students, three postocs and three senior scientists on FoAss
level and above.
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Appendix B
III. Symposia Organized national and international meetings
In the organization committee and Symposium Chairman for a number of national and international
conferences. Elected as Program chair for The Biomaterial and Interface program at AVS 2008, Boston
IV. National and international collaborations
•Prof. Bo Liedberg, Linköping Univ., on sensors, surface modifications and biomimetics within the SSFfunded Biomimetic Material Science program (BIOMICS) •Prof. Lars Samuelson and Prof. Lars Montelius,
Lund Univ, on nanotechnology for life science application strongly linked to e.g. the SSF funded Strategic
research centre Nanowires.•Prof. Carl Borrebeack and Assoc. Prof. Christer Wingren, Lund Univ, which is
linked to the SSF funded strategic research centre Create Health. •Prof Hongqi Xu, Lund Univ, on
theoretical analysis of nanoplasmonic sensors.•Assoc. Prof Tommy Nylander, Physical Chemistry, Lund
Univ, on combined QCM-D and ellipsometry analysis of supported lipid assemblies.•Docent P. Sjövall SP,
adjunct professor (20%) in my group and linked to the EU program Nanobiomaps
(www.nanobiomaps.org).•Prof. M. Käll, Chalmers, on nanoplasmonics and SERS•Prof. Bengt Kasemo,
Chalmers, on supported lipid bilayers and tethered liposomes.•Prof. Peter Brzezinski, Stockholm Univ, on
membrane residing ion channels. •Prof. Per Kjellbom, Lund Univ. on activity measurements of
aquaporines.•Prof. J. Vörös, ETH, Zürich, Switzerland, on DNA and protein microarrays.•Assoc. Prof. D.
Stamou, Copenhagen Univ., Denmark, on lipid based surface modifications and SNARE-mediated vesicle
fusion.•Assoc. Prof. Duncan Sutherland, Aarhus Univ, Denmark, on nanoplasmonics and surface
modifications.•Prof. S. Zaucher and Prof. A. Chilkoti, at Duke Univ, US, on nanoplasmonics.•Prof. H. Xu,
Chinese Academy of Science, Beijing, on nanoplasmonics and SERS.
9) Other merits of relevance
Scientific output
More than 60 peer reviewed publications, cited more than 1600 times, h-index of 21. Editorial board for
Biointerphases, Journal Reviews (>20 annually) Since 2000: e.g. JACS, Anal. Chem., Angew. Chemie,
Langmuir, Small, Nanotechnology…, 6 patents or patent applications
Research program leader
Coordinator for the Chalmers/Lund part of the SSF-funded Biomimetic Material Science Program (Prof. B.
Liedberg) and for the Life Science part of the SSF funded Nanowire excellence centre (Prof. L Samuelson).
Awards
Winner of Innovation Cup 1998
Winner of Akzo Nobels Nordic Research award 2002
Awarded the SSF INGVAR (Individual Grant for the Advancement of Research Leaders) grant 2005
Nominated (Q-Sense) to Stora teknikpriset 2007
Commissions of trust
2000-: Scientific advisor at Q-Sense AB, Göteborg
2003-: Board member in LayerLab AB, Göteborg
2001-: Board member; SSF Program Biomimetic Material Science Program
2006/2007: Member of the SSF strategy groups in Materials and Life Science
2005- Member of “Rådet för forskningutveckling” at Lund University, working with long term strategic
plans for Lund university.
Entrepreneurial achievements
One of the co-founders (two patents) of Q-Sense AB (www.q-sense.com), who has commercialized the
QCM-D system (2 patents and 1 patent application). The key inventor (three patent applications) behind
LayerLab AB (www.layerlab.com) which was initiated together with Chalmers School of Entrepreneurship
during spring 2003 (3 patent applications).
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Appendix B
CV for Mikael Käll
ACADEMIC DEGREES etc.
Professor of Physics,
Docent in Physics
PhD (teknologie doktor)
Licenciate of Engineering (tekn. lic.)
MSc Engineering Physics, Chalmers (civ.ing)
2006-12-01
2000-05-01
1995-06-22
1993-06-11
1989-09-30
APPOINTMENTS etc.
Present employment
Head of Division of BioNanoPhotonics, Dep. of Applied
Physics, Chalmers (permanent, ~70% research), from Jan. 1
2007.
Temporary appointments
Texas Instruments Guest Professor, Rice University, Houston,
2005-01-15 – 07-15
Guest researcher
Donostia International Physics Center, San Sebastian, Spain,
June 2004
Scientific Manager
SWEGENE Center for Biophysical Imaging, Chalmers, from
2003-01-01
Previous periods of employments Lecturer/Associate Professor, 2003-06-01–2006-1-31
Applied Physics, Chalmers
Project leader: 2001-06-01 – 2003-05-31
Applied Physics, Chalmers
Assistant professor: 1997-06-01 – 2001-05-31
Applied Physics, Chalmers
Research assistant 1997-01-01 – 1997-05-31
Applied Physics, Chalmers
NFR Post doc: 1995-09-01 – 1996-12-31
Risø National Lab., Denmark
Graduate student: 1989-11-01 – 1995-08-31
Exp. Physics, Chalmers
SCIENTIFIC INTERESTS & COMPETENCES
The physics of surface plasmons in nanoscale metal structures, and their dependence on size,
shape and electromagnetic interactions. Interactions between molecules and nanoparticular matter.
Electrodynamic simulations/theory of nanoparticulate matter. Applications of plasmon resonances
for surface-enhanced spectroscopy, bio/chemosensing, and metamaterials. Novel light-microscopy
methods, such as single molecule fluorescence techniques, laser tweezers and Raman imaging, for
optical visualization of cellular and molecular processes. Development of quantitative bioimaging
methods for systems biology. Physics of strongly correlated electron systems, such as HTS
cuprates.
13
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Appendix B
ACHIEVEMENTS
• Author / co-author of ~100 refereed journal articles, ~1800 citations.
• Internationally recognized contributions to nanooptics, plasmonics and Raman spectroscopy;
~40 invited talks at international conferences and universities 2000-2007.
• Proven ability to switch field (from Solid State Physics to Nanooptics & Biophysical Imaging)
and rapidly reach the research frontier.
• Proven ability to initiate and co-ordinate interdisciplinary networks, collaborations and
programs. Manager for “Swegene Centre for Biophysical Imaging”.
• INGVAR (Individual grant for the advancement of research leaders) award from the Swedish
Foundation for Strategic Research 2005. Albert Wallins Science Price 2005 from the Royal
Society of Arts and Sciences in Göteborg.
• Continuous VR grants 2000 - >2007.
• Referee for ~15 international journals (e.g. Nature, PRL, JACS); reviewer for the Swedish
Research Council (VR), National Science Foundation (US), Swiss Research Council, Research
Grants Council Hong Kong, Biological Research Council UK.
• Member of EU FP6 Networks of Excellence Plasmo-Nano-Devices and Phoremost.
GRADUATED PHD STUDENTS WITH KÄLL AS MAIN SUPERVISOR
Hongxing Xu (PhD March 15, 2002); Erik J. Bjerneld (PhD December 20, 2002); Juris Prikulis
(PhD November 28, 2003); Fredrik Svedberg (PhD June 2, 2006); Tomas Rindzevicius (PhD
January 26, 2007).
10 most cited publications of relevance to application
H. Xu, E.J. Bjerneld, M. Käll, and L. Börjesson, ”Spectroscopy of Single Hemoglobin Molecules by Surface
Enhanced Raman Scattering”, Physical Review Letters 83, 4357-4360 (1999). 321 citations
H. Xu, J. Aizpurua, M. Käll, and P. Apell, “Electromagnetic contributions to single-molecule sensitivity in surfaceenhanced Raman scattering”, Physical Review E 62, 4318-4324 (2000). 207 citations
J. Aizpurua, P. Hanarp, D.S. Sutherland, M. Käll, G.W. Bryant, and F.J. García de Abajo, “Optical properties of gold
nanorings”, Physical Review Letters 90, # 057401 (2003). 89 citations
C.L. Haynes, A.D. McFarland, L. Zhao, R.P. Van Duyne, G.C. Schatz, L. Gunnarsson, J. Prikulis, B. Kasemo, and M.
Käll, “Nanoparticle optics: The importance of radiative dipole coupling in two-dimensional nanoparticle arrays”,
Journal of Physical Chemistry B 107, 7337-7342 (2003). 87 citations
H. Xu and M. Käll, “Surface-plasmon enhanced optical forces in silver nanoaggregates”, Physical Review Letters 89,
# 246802 (2002). 66 citations
E.J. Bjerneld, Z. Földes-Papp, M. Käll, and R. Rigler, “Single-molecule surface-enhanced Raman and fluorescence
correlation spectroscopy of horse-radish peroxidase”, J. Phys. Chem. B 106, 1213-1218 (2002). 51 citations
L. Gunnarsson, E.J. Bjerneld, H. Xu, S.Petronis, B. Kasemo and M. Käll, “Interparticle-coupling effects in
Nanofabricated Substrates for Surface Enhanced Raman Scattering”, Appl. Phys. Lett. 78, 802-804 (2001). 45
citations.
L. Gunnarsson, T. Rindzevicius, J. Prikulis, B. Kasemo, M. Käll, S. Zou, and G.C. Schatz, “Confined plasmons in
nanofabricated single silver particle pairs – Experimental observations of strong interparticle interactions”, Journal of
Physical Chemistry B 109, 1079-1087 (2005). 32 citations
P. Hanarp, M. Käll, and D. Sutherland; Optical properties of short-range ordered arrays of nanometer gold disks
prepared by colloidal lithography”, Journal of Physical Chemistry B 107, 5768-5772 (2003). 30 citations
H. Xu and M. Käll, “Polarization dependent surface-enhanced Raman spectroscopy of isolated silver
nanoaggregates”, ChemPhysChem 4, 1001-1005 (2003). 28 citations
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Appendix B
CV for Lisbeth Cecilia Olsson
Doctoral degree: 1994
Postdoctoral periods: 1994-1996 Post doc at Department of Biotechnology, Technical University of
Denmark
Qualification as Associate Professor : 1998 qualified as unremunerated docent at Lund University and
the same year I received a faculty position (associate professor) at DTU
Current position, appointment period and time for research in the position
Professor in Industrial Fermentation Physiology, appointed 2006, 50 % research
Previous positions and periods of appointment
2005-2006 Director of studies at BioCentrum-DTU
2008- Professor in Bioprocess Technology, Department of Chemical and Biological Engineering at
Chalmers University
Parental leave, service in the Armed Forces or similar periods, and research time deductible for such
purposes: Maternity leave for 6 months in 2001
Number of people awarded doctorates for whom the participating researcher has been a supervisor
Kasper Møller, 1998-2001
Christophe Roca, 1999-2002
Henning Jørgensen, 2000-2003
Rasmus Devantier, 2001-2005
Martin Haack, 2001-2005
Kristian Krogh, 2002Astrid Mørkeberg, 2002Natalija Andersen, 2004-2007
Renata Usaite, 2004Maya Petrova Doneva, 2004Marta Papini, 2007assistant supervisor to 12 PhD-students
Number of postdoctoral researchers who are or have been collaborating with the researcher in the
research group
Hans Peter Smits, 1996-1999
Magnus Ehlde, 1996-1997
Yijun Wang, 1997
Aradhana Srivastava, 1997-1998
Attawut Impoolsup, 2002
Paul Christakopoulos, 2003
Anna Eliasson, 2000-2003
Kasper Møller, 2001-2003
Gianni Panagitou, 2004Goutham Vemuri, 2006Valeria Mapelli, 2007National and international assignments of importance
•
International committee activities:
EFB Microbial Physiology Section and EFB Working Group on Measurement and Control
15
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Appendix B
IEA Task 39, Danish representative
ERA.Industrial Biotechnology, EU, Danish representative
• National committee activities:
Working group for development of “kort videregående uddannelse, laboratorieområdet (1999)
Biotechnology contact committee (IDA, 2004-2005)
Danish Biotechnology Society, founding board member (IDA, 2006)
Network leader, biological production, LMC (2006-2007)
• Editorial tasks:
Biofuels, Bioproducts & Biorefining, Consultant Editor
Advances in Biochemical Engineering/Biotechnology, volume editor for volume 108, Biofuels
Biotechnology for Biofuels, Associate editor
• Referee for grant applications:
United States Department of Agriculture, Australian Research Council Research Committee,
Fonds voor Wetenschappelik Onderzoek –Vlaanderen, Belgium, Austrian Science Fund (FWF),
VINNOVA, Sweden
Vetenskabsrådet, Sweden
•
PhD-thesis evaluator:
Techanical University of Denmark, 12 PhD theses
Royal Veterinary and Agricultural University, Denmark, 1 PhD thesis
Lund University, Sweden; 2 PhD theses and 1 Licentiate thesis
Helsinki University of Technology, Finland, 1 PhD thesis
Chalmers University, Sweden; 2 PhD theses
Göteborgs Univesitet, Sweden; 1 PhD thesis
University of Matanzas, Cuba, 1 PhD thesis
Delft Univeristy of Technology, 1 PhD thesis
• Evaluator for personnel:
Assistant and associate professorships at DTU, Professorship at KVL, PhD-studentships at Research School
FOOD, Associate professor at Norwegian University of Science and Technology (NTNU), Academic
promotion EPFL, Switzerland, Research rating, National Research foundation, South Africa
• Conference and workshop organizer:
Physiology on yeasts and filamentous fungi, EFB event 104, chair, 2001
IEA, Current state of fuel ethanol commercialization, chair, 2003
Biological production, Danish Biotechnological Society (chair), 2006
Participated in 10 organising/scientific committees for national and international conferences
Qualifications with respect to collaboration and/or communication of research findings
• Collaborations
Denmark: Department of Chemical Engineering-DTU, Copenhagen University, Faculty of Life Science,
Danisco A/S, Novozymes A/S, Novo Nordisk A/S, Alpharma A/S, Delta Lys og Optic, Technological
Institute.
International: Lund University, University of Wisconsin, Scripps Institute, CIEMAT, National Unversity of
Technology Athens,
• Communication of research findings
89 peer-reviewed publications, 2 book contributions, 6 conference proceedings, 9 popular science
publications; More than 30 invited talks at international conference
16
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Appendix B
CURRICULUM VITAE
_____________________________________________________________________
Owe Orwar
Department of Physical Chemistry
Chalmers University of Technology
Kemivägen 10, 412 96 Göteborg, Sweden
Tel:+46-31-7722778, Fax:+46-31-7722785, email:[email protected]
www.orwarlab.mc2.chalmers.se
EDUCATION AND PROFESSIONAL EXPERIENCE
1988
B.Sc Chemistry, Göteborgs University, Göteborg, Sweden.
1994
Ph.D. Bioanalytical Chemistry Göteborg University, Göteborg, Sweden.
Thesis opponent: Professor Milos Novotny, Indiana University, Bloomington, IN., USA.
1994-1996
Postdoctoral fellow in Biophysical Chemistry with Professor Richard N.
Zare at the Department of Chemistry, Stanford University, CA.
1996-2000
Associate Professor in Chemistry at Göteborg University.
2000-
Professor in Biophysical Chemistry at Chalmers University of Technology.
2003-2004
Visiting Professor Institut Curie, Paris, France
SCIENTIFIC PUBLICATIONS AND INVITED LECTURES
Author and co-author of about 100 scientific publications, twelve of these are published in outstanding scientific
journals, i.e. three in Science, one in Nature, seven in Proceedings of the National Academy of Sciences, USA, and
one in Ann. Rev. Phys. Chem. Invited speaker on >40 occasions.
AWARDS
* Wallenberg graduate student award in 1991
* Adlerbertska graduate student award in 1992
* The Swedish Natural Science Research Council's (NFR) Postdoctoral Award 1994 & 1995
* The Swedish Natural Science Research Council's (NFR) Junior Researcher Award in 1997, 1998, 1999, and 2000
* The Foundation for Strategic Research (SSF) Junior Individual Grant for 1997, 1998, & 1999
* A Hoffman-LaRoche Young Investigator Award in 1997
* Royal Swedish Academy of Sciences Research Fellow 2000-2005
* SSF INGVAR award in 2001
* Pittsburgh society for spectroscopy and Pittsburgh society for analytical chemistry achievement
award 2003
* A SKAPA innovation award in 2003
* The Rotschild-Yvette Mayent-Institut Curie Award 2003
* The Göran Gustafsson Price in 2004
* The Norblad-Ekstrand Medal from the Swedish Chemical Society in 2004
PATENTS AND COMPANIES
Holder and co-inventor of about thirty patents. Orwar is a co-founder (together with Dr. D. Chiu, University of
Washington, and Mr. J. Lindberg, Stockholm) of Cellectricon AB, a company active in the area of cell-based
assays, and microfluidics devoted to development of products in high-throughput drug screening, combinatorial
gene expression, and iRNA screening. Orwar is also a co-founder of Nanoxis AB together with Ahmet Senoglu and
students in Orwar´s research group. Nanoxis focuses on membrane proteomics.
EDITORIAL COMMITMENTS
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Appendix B
Past member of the A-page editorial board of Analytical Chemistry
Frequent reviewer for Analytical Chemistry, JACS, PNAS, Angewandte Chemie, and Langmuir
HONORARY ASSIGNMENTS-RESEARCH PROGRAM EVALUATIONS
Reviewer and research program evaluator for the Austrian National Natural Science Research Council; Fonds zur
Förderung der Wissenschaftlichen Forschung (FWF) in Vienna, Austria.
External group reviewer for the Swedish Natural Science Research Council in chemistry.
Reviewer of applicants for an Assistant Professorship in Analytical Chemistry at Stocholm University in 1999
Reviewer for a Professor Recruitment in Nanotechnology at the University of Aalborg
Reviewer for a Professor Recruitment in Nanotechnology at the University of Trondheim
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Appendix B
Curriculum vitae Björn Åkerman
Date and place of birth: January 23 1957, Malmö, Sweden
Professor, Department of Physical Chemistry, Chalmers University of Technology, S41296
Göteborg, Sweden
Professional preparation
™ 1984-09-10 Master's degree in Engineering physics, Chalmers University of Technology
™ 1990-09-24 Doctor of Philosophy, in Physical chemistry
™ 1997-02-27 Docent
Appointments
™ 1990 Research assistant, Department of Physical Chemistry, Chalmers
™ 1991–1993 Postdoctoral fellow at the Institute of Molecular biology, University of
Oregon, Eugene, Oregon, USA
™ 1993 Project manager, Department of Physical Chemistry, Chalmers;
™ 1994 Assistant Professor, Department of Physical Chemistry, Chalmers
™ 1998 Associate professor in Physical Chemistry, Chalmers
™ 2001 Professor in Biophysical Chemistry, Chalmers
Grants and honours
™ 1991 - 1993 Swedish research council postdoctoral fellowship
™ 1991 - 1993 Fullbright fellowship
™ 1998 Chalmers pedagogical prize
Scientific accomplishments
About 60 scientific publications and 4 book chapters.
PhD-supervision
Anette Lasson 1994
Maja Eriksson 2006
Michal Tokarz 2007
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Appendix B
International collaborations
• K. Cole, National Institute of Standards and Technology. Gels for specific DNA separations.
• L. Letellier, Univeriste de Paris Sud. DNA dynamics in phage-infections
• H. Vogel, Ecole Federale Polytechnique de Lausanne. Immobilisation of DNA oligomers on
surfaces.
• P. Alexandridis, SUNY Buffalo, USA. Polymer dynamics in liquid-crystal gels.
• M. Takahashi, University of Nantes. RecA-DNA interactions.
• E. Tuite, University of Newcastle. DNA photocleavage.
Guest researcher
NIST, Washington DC, June-Aug 1998,
IBBM, University of Paris, 4 months 2001, 2002
20
Kod
2007-14950-54750-89
Name of applicant
Markides, Karin
Date of birth
511124-1021
Title of research programme
Appendix C
List of publications
VRAPS/VR-Direct bilaga 2004.Re
Vetenskapsrådet, SE-103 78 Stockholm, tel. +46 (0)8 546 44 000, [email protected]
Karin Markides 511124-1021
Supra Centre Chalmers
Appendix C
Publications Bengt Norden
10 selected publications*
* Selected from publications during 1992-2007 as relevant for proposal.
Mortensen, K., Brown, W. and Nordén, B. Inverse Melting Transition and Evidence of 3-D
Cubatic Structure in a Block-Copolymer Micellar System
Phys Rev Letters 68 (1992) 2340-2343.
Hiort, C., Lincoln, P. and Nordén, B. DNA Binding of D- and L-[Ru(phen)2DPPZ]2+.
J. Am. Chem. Soc. 115 (1993) 3448-3454.
Egholm, M., Buchardt, O., Christensen, L., Behrens, C., Freier, S.M., Driver, D. A., Berg,
R.H., Kim, S.K., Nordén, B. and Nielsen, P.E. PNA Hybridizes to Complementary
Oligonucleotides Obeying the Watson-Crick Hydrogen Bonding Rules.
Nature, 365 (1993) 566-568.
Wittung, P., Nielsen P.E., Buchardt, Ole., Egholm, M. and Nordén, B. DNA-like Double Helix
formed by Peptide Nucleic Acid - Direct observation of helical seeding.
Nature, 368 (1994) 561-563.
Thorén, P.E.G., Persson, D., Karlsson, M. and Nordén, B. The Antennapedia Penetratin
Peptide Translocates Across Lipid Bilayers: The First Direct Observation
FEBS Letters 482 (2000) 265-268.
Frykholm K, Morimatsu K, Nordén B Conserved conformation of RecA protein after
executing the DNA strand-exchange reaction. A site-specific linear dichroism structure study.
Biochemistry 45 (2006) 11172-8.
Nordell P, Westerlund F, Wilhelmsson LM, Nordén B, Lincoln P Kinetic Recognition of ATRich DNA by Ruthenium Complexes.
Angew Chem Int 46 (2007) 2203-2206
John Tumpane, Peter Sandin, Ravindra Kumar, Vicki E.C. Powers, Erik Lundberg, Nittaya
Gale, Piero Baglioni, Jean-Marie Lehn, Bo Albinsson, Per Lincoln, L. Marcus Wilhelmsson,
Tom Brown, Bengt Nordén Addressable High-Information-Density DNA Nanostructures
Chem. Phys. Letters. 440 (2007) 125-129.
Thorén, P.E.G., Persson, D., Isakson, P., Goksar, M., Önfelt, A., Nordén, B. Uptake of
Analogs of Penetratin, Tat(48-60) and Oligoarginine in Live Cells.
Biochem. Biophys. Res. Comm. 307 (2003) 100-107.
John Tumpane, Ravindra Kumar, Erik P. Lundberg, Peter Sandin, Nittaya Gale, Iris S.
Nandhakumar, Bo Albinsson, Per Lincoln, L. Marcus Wilhelmsson, Tom Brown and Bengt
Nordén. Triplex Addressability as a Basis for Functional DNA Nanostructure Devices
Nano Letters 00 (2007) 0000-0000.
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Appendix C
2003-2007#
# Also 2000-2002 included as motivated by relevancy to proposal.
299
Ray, A. and Nordén, B. Peptide nucleic acid (PNA): its medical and biotechnical
applications and promise for the future. FASEB J. 14 (2000) 1041-1060.
300
Gisselfält, K., Lincoln, P., Nordén, B. and Jonsson, M. Interactions of
tris(phenanthroline)ruthenium(II) enantiomers with DNA: effects on helix flexibility
studied by the electrophoretic behaviour of reptating DNA in agarose gel.
J.Phys.Chem 104 (2000) 3651-3659.
301
Önfelt, B., Lincoln, P., Nordén, B., Baskin, J.S. and Zewail, A.H. Femtosecond linear
dichroism of DNA-intercalating chromophores: solvation and charge separation
dynamics of ∆-[Ru(phen)2DPPZ]2+ systems.
Proc. Natl. Acad. Sci U.S. 97 (2000) 5708-5713.
302
Zolotaryuk, A.V., Christiansen, P.L., Nordén, B., Savin, A.V. and Zolotaryuk, Y.
Pendulum as a model system for driven rotation in molecular nanoscale machines
Phys Rev E. 61 (2000) 3256-3259.
303
Ratilainen, T., Holmén, A., Tuite, E. , Nielsen, P.E. and Nordén, B. Thermodynamics
of sequence-specific binding of PNA to DNA. Biochemistry 39 (2000) 7781-7791.
304
Tuite, E., Lincoln, P., Olofsson, J., Becker, H.C., Önfelt, B., Erts, D. and Nordén, B.
Probing DNA Conductivity with Photoinduced Electron Trander and Scanning
Tunneling Microscopy J.Biol.Struct.Dyn. 11 (2000) 277-283.
305
Quesada, E. Ardhammar, M., Nordén, B. Miesch, M., Duportail, G., Bonzi-Coulibaly,
Y., Nakatani, Y, and Ourisson, G. Synthesis and Fluorescence properties of Novel
Transmembrane Probes and Determination of Their Orientation in Vesicles
Helvetica Chimica Acta 83 (2000) 2464-2476.
306
Thorén, P.E.G., Persson, D., Karlsson, M. and Nordén, B. The Antennapedia
Penetratin peptide Translocates Across Lipid Bilayers: The First Direct Observation
FEBS Letters 482 (2000) 265-268.
307
Ardhammar, M., Nordén, B. and Kurucsev, T. “DNA-Drug Interactions” (Invited
Review) Circular Dichroism: Principles and Applications (Ed. Berova, N., Nakanishi,
K. and Woody, R.W.). John Wiley & Sons, New York 2000, pp 741-768.
308
Becker, H.C. and Nordén, B. DNA Binding Thermodynamics and Sequence Specificity
of Chiral Piperazinecarbonyloxyalkyl Derivatives of Anthracene and Pyrene
J.Am.Chem.Soc. 122 (2000) 8344-8349.
309
Wilhelmsson, L.M., Holmén, A., Lincoln, P., Nielsen, P.E. and Nordén, B. A Highly
Fluorescent DNA Base Analogue that Forms Watson-Crick Base Pairs with Guanine
J.Am.Chem.Soc. 123 (2001) 2434-2435.
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Appendix C
310
Önfelt, B. , Lincoln, P. and Nordén, B. Enantioselective DNA Threading Dynamics
by Phenazine-Linked [Ru(phen)2dppz]2+ Dimers
J.Am.Chem.Soc. 124 (2001) 3630-3637.
311
Kim, H.-K., Tuite, E., Nordén, B. and Ninham, B. Co-Ion Dependence of DNA
Nuclease Activity Suggests Hydrophobic Cavitation as a Potential Source of
Activation Energy Eur. Phys. J. E 4 (2001) 411-417.
312
Tuite, E., Olofsson, J., Önfelt, B. Lincoln, P., Nordén Influence of DNA on Charge
Separation Processes of Ruthenium(II) Complexes J. Inorg. Biochem. 86 (2001) 109.
313
Eriksson, M. and Nordén, B. Linear and Circular Dichroism of Drug-Nucleic Acid
Complexes Meth.Enz. 340 (2001) 68-98.
314
Ratilainen, T., Lincoln, P. and Nordén, B. A simple model for gene-targeting
Biophys.J. 81 (2001) 2876-2885.
315
Ardhammar, M. , Lincoln, P. and Nordén, B. Ligand Substituents of Ruthenium
Dipyridophenazine Complexes Sensitively Determine Orientation in Liposome
Membrane J. Phys. Chem. 105 (2001) 11363-11368.
316
Coates, C.G., Olofsson J., Lincoln, P., Coletti, M., McGarvey, J.J., Önfelt, B., Nordén,
B., Tuite, E., Matousek, P. and Parker, W. Picosecond Time-Resolved Resonance
Raman Probing the Light Switch States of [Ru(Phen)2dppz]2+
J. Phys. Chem. 105 (2001) 12653-12664.
317
Persson, D., Thorén, P.E.G. and Nordén, B. Penetratin-induced Aggregation and
Subsequent Dissociation of Negatively Charged Phospholipid Vesicles
FEBS Letters 505 (2001) 307-312.
318
Höök, F., Ray, A., Nordén, B. and Kasemo, B. Characterization of PNA and DNA
Immobilization and Subsequent Hybridization with DNA Using Acoustic-Shear-Wave
Attenuation Measurements. Langmuir 17 (2001) 8305-8312
319
Nordén, B., Zolotaryuk, Y., Christiansen, P.L. and Zolotaryuk, A.V. Ratchet Device
with Broken Friction Symmetry Appl. Phys. Lett. 80 (2002) 2601-2603
320
Rodger, A., Nordén, B., Rodger, P.M. and Bates P.B. DNA as a Catalyst and
Catalytic Template for the Racemisation of Metal Tris-Phenanthroline Complexes Eur.
J. Inorg. Chem. 2002, 49-53.
321
Gillgren, H., Stenstam, A., Ardhammar, M., Nordén, B., Sparr, E. and Ulvenlund, S.
Morphology and Molecular Conformation in Thin Films of Poly- -methyl-Lglutamate at the Air-Water Interface. Langmuir 18 (2002) 462-469
322
Nordén, B., Zolotaryuk, Y., Christiansen, P.L. and Zolotaryuk, A.V. Ratchet due to
Broken Friction Symmetry Phys. Rev. E. 65 (2002) Art. # 011110; Noise Put to Work
(commentatory) Nature 2002
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Appendix C
323
Wilhelmsson, L.M., Nordén, B., Mukherhee, K. Dulay, M.T. and Zare, R,N. Genetic
Screening Using the Colour Change of a PNA-DNA-Hybrid Binding Cyanine Dye,
Nucleic Acids research 30 (2002) 00
324
Kim, H.-K., Morimatsu, K., Nordén, B., Ardhammar, M. and Takahashi, M. ADP
Stabilizes the Human Rad51-single stranded DNA Complex and Promotes Its DNA
Annealing Activity. Genes to Cells 7 (2002) 1125-1134.
325
Ratilainen, T. and Nordén, B. Thermodynamics of PNA Interactions with DNA and
RNA in Methods in Molecular Biology: Peptide Nucleic Acids (Ed. P.E. Nielsen)
Humana Press, Totowa, New Jersey 2002, pp 59-88.
326
Gawronski, J., Brzostowska, M., Gawronska, K., Koput, J. , Rychlewska, U. and
Nordén, B. Structure, Electronic Transitions and Exciton Coupling of
Pyromellitdiimide (1,2,4,5-Benzenetetracarboxydiimide) Dimers and Trimers
Chem.Eur.J. 8 (2002) 2484-2494 and ibid. 2833.
327
Rodger, A., Rajena, J., Mortimer, R., Andrews, T., Hirst, J.B., Gilbert, A.T.B.,
Marrington, R., Dafforn, T.R., Halsall, D.J., Ardhammar, M., Nordén, B., Woolhead,
C.A., Robinson, T.P., Kazlauskaite, J., Seymour, M., Perez, Hannon, M.J. Flow
Oriented Linear Dichroism to probe Protein Orientation in Membrane Environments
Phys.Chem.Chem.Phys. 4 (2002) 4051-4057.
328
Olofsson, J., Önfelt, B., Lincoln, P., Nordén, B., Tuite, E., Matousek, P. and Parker,
A.W. Pico-second Kerr-Gated Time-Resolved Raman Spectroscopy of the
[Ru(phen)2dppz]2+ Chromophore Interacting with DNA
J.Inorg.Biochem. 91 (2002) 286-297.
329
Önfelt, B., Gostring, P., Lincoln, P., Nordén, B. Cell Studies of the DNA BisIntercalator Delta-Delta [mu-C4(cpdppz)2(phen)4Ru2dppz]4+: Toxic Effects and
Properties as a Light-Emitting DNA Probe in V79 Chinese Hamster Cells.
Mutagenesis 17 (2002) 317-320.
330
Grimm, G.N., Boutorine, A.S., Lincoln, P. , Nordén, B. and Hélène, C. Formation of
DNA Triple Helices by an Oligonucleotide Conjugated to a Fluorescent Ruthenium
Complex Chem. Biochem. 3 (2002) 324-331.
331
Wilhelmsson, L.M., Westerlund, F., Lincoln, P. and Nordén, B. DNA-Binding of
Semi-Rigid Binuclear Ruthenium Complex Delta-Delta [mu-11,11'bidppz)(phen)4Ru2]4+: Extremely Slow Intercalation Kinetics
J.Am.Chem.Soc. 124 (2002) 12092-12093.
332
Morimatsu, K., Takahashi, M. and Nordén, B. Arrangement of RecA Protein in Its
Active Filament Determined by Polarized-Light Spectroscopy
Proc. Natl. Acad. Sci. USA 99 (2002) 11688-11693.
333
Ardhammar, M., Lincoln, P. and Nordén, B. Invisible Liposomes: Refractive Index
Matching with Sucrose Enables Flow Dichroism Assessment of Peptide Orientation in
Lipid Vesicle Membranes Proc. Natl. Acad. Sci. USA 99 (2002) 15313-15317.
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Appendix C
334
Önfelt, B., Olofsson, J., Lincoln, P. and Nordén, B. Picosecond and Steady-State
Emission of [Ru(dppz)(phen)2]2+ in Glycerol: Anomalous Temperature Dependence
Reveals Fast Excited State Equilibrium. J.Phys.Chem. 107 (2003) 1000-1009.
335
Westerlund, F. , Wilhelmsson, L.M., Lincoln, P. and Nordén, B. Micelle-Sequestered
Dissociation of Cationic DNA-Intercalated Drugs: Unexpected Surfactant-Induced
Rate Enhancement J. Am. Chem. Soc. 125 (2003) 3773-3779.
336
Persson, D., Thorén, P., Herner, M., Lincoln, P. and Nordén, B. Application of a Novel
Analysis To Measure the Binding of the Membrane-Translocating Peptide Penetratin
to Negatively Charged Liposomes Biochemistry 42 (2003) 421-429.
337
Brattwall, C.E.B., Lincoln, P. and Nordén, B. Orientation and Conformation of CellPenetrating Peptide Penetratin in Phospholipid Vesicle Membranes Determined by
Polarized-Light Spectroscopy J. Am. Chem. Soc. 125 (2003) 14214-14215.
338
Wilhelmsson, L.M., Esbjörner, E.K., Westerlund, F., Nordén, B. and Lincoln, P.
Meso Stereoisomer as a Probe of Enantioselective Threading Intercalation of
Semirigid Ruthenium Complex J. Phys. Chem. B 107 (2003) 11784-11793.
339
Yun, B.H., Kim, J.O., Lee, B.W., Lincoln, P., Nordén, B., Kim, J.M., Kim, S.K.
Simultaneous binding of Ruthenium(II) [(1,10-phenanthroline)2(dipyridophenazine}]2+
and Minor Groove Binder 4,6-Diamidino-2-phenylindole to poly[d(A-T) 2] at high
binding Densities: Observation of Fluorescence Resonance Energy Transfer Across
the DNA Stem. J. Phys. Chem. B 107 (2003) 9858-9864.
340
Thorén, P.E.G., Persson, D., Isakson, P., Goksar, M., Önfelt, A., Nordén, B. Uptake
of Analogs of Penetratin, Tat(48-60) and Oligoarginine in Live Cells
Biochem. Biophys. Res. Comm. 307 (2003) 100-107.
341
Wilhelmsson, L.M., Sandin, P., Holmén, A., Albinsson, B., Lincoln, P. and Nordén, B.
Photophysical characterization of fluorescent DNA base analogue, tC.
J. Phys. Chem. 107 (2003) 9094-9101.
342
Thorén, P.E.G., Persson, D., Esbjörner, E.K., Goksör, M., Lincoln, P. and Nordén, B.
Membrane Binding and Translocation of Cell-Penetrating Peptides.
Biochemistry 43 (2004) 3471-3489.
343
Persson, D., Thorén, P., Lincoln, P., Nordén, B. Vesicle membrane interactions of
penetratin analogs . Biochemistry 43 (2004) 11045-11055.
344
Persson, D., Thorén, P., Esbjörner, E.K., Goksör, M., Lincoln, P. and Nordén, B.
Vesicle size-dependent translocation of penetratin analogs across lipid membranes .
Biochimica et Biophysica Acta 1665 (2004) 142– 155
345
Cusumano M, Di Pietro ML, Giannetto A, Nicolo F, Norden B, Lincoln P Ambivalent
intercalators for DNA: L-shaped platinum(II) complexes
Inorganic Chemistry 43 (2004) 2416-2421 .
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Appendix C
346
Engman KC, Sandin P, Osborne S, Brown T, Billeter M, Lincoln P, Norden B,
Albinsson B, Wilhelmsson LM. DNA adopts normal B-form upon incorporation of
highly fluorescent DNA base analogue tC: NMR structure and UV-Vis spectroscopy
characterization.
Nucleic Acids Res. 32 (2004) 5087-95.
347
James PL, Le Strat L, Ellervik U, Brattwall C, Norden B, Brown T, Fox KR.
Effects of a hairpin polyamide on DNA melting: comparison with distamycin
and Hoechst 33258.
Biophys Chem. 111 (2004) 205-12.
348
Thorén, P.E.G., Persson, D., Lincoln, P. and Nordén, B. Membrane destabilizing
properties of cell-penetrating peptides: correlations with cellular uptake.
Biophys.Chem. 114 (2005) 169-179.
349
Westerlund, F., Wilhelmsson, LM, Nordén, B, Lincoln, P. Monitoring the DNA
binding kinetics of a binuclear ruthenium complex by energy transfer: Evidence for
slow shuffling J. Phys. Chem B 109 (2005) 21140-21144.
350
Westerlund, F. Pierard, F, Eng, MP, Nordén, B., Lincoln, P. Enantioselective
luminescence quenching of DNA light-switch [Ru(dppz)(phen)2]2+ by electron
transfer to structural homologue [Ru(phendione)(phen)2]2+
J. Phys. Chem. B 109 (2005) 17327-17332.
351
Ceasar, C., Esbjörner, EK, Lincoln, P. and Nordén, B. Membrane interactions of cellpenetrating peptides probed by tryptophan fluorescence and dichroism techniques.
Correlations of structure to cellular uptake. Biochemistry 45 (2006) 7682-92
352
Frykholm K, Morimatsu K, Nordén B Conserved conformation of RecA protein after
executing the DNA strand-exchange reaction. A site-specific linear dichroism
structure study. Biochemistry 45 (2006) 11172-8.
353
Caesar CE, Johnsson R, Ellervik U, Fox KR, Lincoln P, Nordén B A polarized-light
spectroscopy study of interactions of a hairpin polyamide with DNA.
Biophys J 91 (2006) 904-11.
354
Nordell P, Westerlund F, Wilhelmsson LM, Nordén B, Lincoln P Kinetic Recognition
of AT-Rich DNA by Ruthenium Complexes. Angew Chem Int 46 (2007) 2203-2206
355
Elin K. Esbjörner, Astrid Gräslund and Bengt Nordén Membrane Interactions of CellPenetrating Peptides Handbook of cell-Penetrating Peptides CRC press Cambridge
2007, pp 109-137.
356
Takahashi M, Maraboeuf F, Morimatsu K, Selmane T, Fleury F, Norden B
Calorimetric analysis of binding of two consecutive DNA strands to RecA protein
illuminates mechanism for recognition of homology. J Mol Biol 365 (2007) 603-613.
357
John Tumpane, Peter Sandin, Ravindra Kumar, Vicki E.C. Powers, Erik Lundberg,
Nittaya Gale, Piero Baglioni, Jean-Marie Lehn, Bo Albinsson, Per Lincoln, L.
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Appendix C
Marcus Wilhelmsson, Tom Brown, Bengt Nordén Addressable High-InformationDensity DNA Nanostructures Chem. Phys. Letters. 440 (2007) 125-129.
358
Elin K. Esbjörner, Per Lincoln, Bengt Nordén Counterion-mediated membrane
penetration: Cationic cell-penetrating peptides overcome Born energy barrier by ionpairing with phospholipids
Biochim. Biophys. Acta - Biomembranes 1768 (2007) 1550-1558.
359
Elin K. Esbjörner, Christina Caesar, Bo Albinsson, Per Lincoln and Bengt Nordén
Tryptophan Orientation in Model Lipid Membranes
Biochem. Biophys. Res. Comm. 361 (2007) 645-650
360
Frida Svensson, Per Lincoln, Bengt Nordén and Elin K. Esbjörner Retinoid
Chromophores as Probes of Membrane Lipid Order
J. Phys. Chem. B 111 (2007) 10839-10848.
361
Elin K. Esbjörner, Kamila Oglecka, Per Lincoln and Bengt Nordén Membrane
Binding of pH-sensitive Influenza Fusion Peptides. Positioning, Configuration and
Induced Leakage in Lipid Vesicle Model
Biochemistry 00 (2007) 0000-0000.
362
G. Stengel, J. P. Gill, P. Sandin, L. M. Wilhelmsson, B. Albinsson, B. Norden, D.
Millar Conformational Dynamics of DNA Polymerase Probed with a Novel
Fluorescent DNA Base Analog Biochemistry 46 (2007) 12289-12297.
363
John Tumpane, Ravindra Kumar, Erik P. Lundberg, Peter Sandin, Nittaya Gale, Iris S.
Nandhakumar, Bo Albinsson, Per Lincoln, L. Marcus Wilhelmsson, Tom Brown and
Bengt Nordén. Triplex Addressability as a Basis for Functional DNA Nanostructure
Devices Nano Letters 00 (2007) 0000-0000.
376
Elin K. Esbjörner, Helene Åmand, Kristina Fant and Bengt Nordén Stimulated
Endocytosis in Uptake of Penetratin Peptides: Influence of Arginines and Lysines .
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Appendix C
Publications Bo Albinsson,
2003-2007 + important (*)
Publications in refereed journals.
1. (*) Albinsson, B., Kubista, M., Nordén, B., Thulstrup, E. W. Near-Ultraviolet
Electronic Transitions of the Tryptophan Chromophore: Linear Dichroism,
Fluorescence Anisotropy and Magnetic Circular Dichroism Spectra of Some
Indole Derivatives. J. Phys. Chem. 93 (1989) 6646-6654.
2. (*) Kubista, M., Sjöback, R., Albinsson, B. Determination of Equilibrium
Constants by Chemometric Analysis of Spectroscopic Data. Anal. Chem. 65
(1993) 994-998.
3. (*) Larsson, A., Carlsson, C., Jonsson M., Albinsson, B. Characterization of
the Binding of the Fluorescent Dyes YO and YOYO to DNA by Polarized Light
Spectroscopy. J. Am. Chem. Soc. 116 (1994) 8459-8465.
4. (*) Albinsson, B., Teramae, H., Downing, J. W., Michl, J. Conformers of
Saturated Chains: Matrix-Isolation, IR and UV Spectra of n-Si4Me10. Chem.
Eur. J 2 (1996) 529-538.
5. (*) Holmén, A; Nordén, B.; Albinsson, B. The Electronic Transition Moments
of 2-Aminopurine J. Am. Chem. Soc. 119 (1997) 3114-3121.
6. (*) Kilså Jensen, K; van Berlekom, S.; Kajanus, J.; Mårtensson, J.; Albinsson,
B. Mediated Energy Transfer in Covalently Linked Porphyrin Dimers J. Phys.
Chem. A 101 (1997) 2218-2220.
7. (*) Kilså, K; Kajanus, J.; Mårtensson, J.; Albinsson, B. Mediated Electronic
Coupling: Energy Transfer in Porphyrin Dimers Enhanced by the Bridging
Chromphore. J. Phys. Chem. B. 103 (1999) 7329-7339.
8. (*) Kilså, K.; Kajanus, J.; Macpherson, A. N.; Mårtensson, J.; Albinsson, B.
Bridge-Dependent Electron Transfer in Porphyrin-Based Donor-BridgeAcceptor Systems J. Am. Chem. Soc. 123 (2001) 3069-3080.
9. (*) Kyrychenko, A.; Albinsson, B. Conformer-Dependent Electronic
Coupling for Long-Range Triplet Energy Transfer in Donor-Bridge-Acceptor
Pophyrin Dimers. Chem. Phys. Lett. 366 (2002) 291-299.
10. Andréasson, J.; Kodis, G.; Ljungdahl, T.; Moore, A. L.; Moore, T. A.; Gust,
D.; Mårtensson, J.; Albinsson, B. Photoinduced Hole Transfer from the
Triplet State in a Porphyrin-Based Donor-Bridge-Acceptor System. J. Phys.
Chem. A 107 (2003) 8825-8833
11. Wilhelmsson, L. M., Sandin, P., Holmén, A., Albinsson, B., Lincoln, P. and
Nordén, B. Photophysical Characterization of Fluorescent DNA Base
Analogue, tC. J. Phys. Chem. B 107 (2003) 9094-9101
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Appendix C
12. Pettersson, K.; Kilså, K.; Mårtensson, J.; Albinsson, B. Intersystem crossing
versus electron transfer in porphyrin-based donor-bridge-acceptor systems:
Influence of a paramagnetic species J. Am. Chem. Soc. 126 (2004) 67106719.
13. Stomberg, R.; Albinsson, B.; Langer, V.; Li, S; Lundquist, K. (Z)-2,3-bis(3,4dimethoxyphenyl)-2-propen-1-ol Acta Cryst. E60 (2004) o804–o806.
14. Engman, C.; Sandin, P.; Osborne. S.; Brown, T.; Billeter, M.; Lincoln, P.;
Nordén, B.; Albinsson, B.; Wilhelmsson, L. M. DNA adopts normal B-form
upon incorporation of highly fluorescent DNA base analogue tC - NMR
structure and UV-Vis spectroscopy characterisation. Nucleic Acid Research
32 (2004) 5087-5095
15. Winters, M.; Pettersson, K.; Mårtensson, J.; Albinsson, B. Temperature
Dependence of Long-range Electron Transfer: Hopping vs. Superexchange
Mechanism. Chem. Eur. J. 11 (2005) 562-573.
16. Eng, M.; Andréasson, J.; Mårtensson, J; Albinsson, B. The Triplet
Photphysics of Gold(III) Porphyrins. J. Phys. Chem. A 109 (2005) 1776-1784.
17. Sandin, P.; Wilhelmsson, L. M.; Lincoln, P.; Brown, T.; Albinsson, B.
Fluorescent Properties of DNA Base Analog tC upon Incorporation into DNA
- Negligible Influence of Neighboring Bases on Fluorescence Quantum Yield.
Nucl. Acids Res. 33 (2005) 5019-5025
18. Andréasson, J.; Terazono, Y.; Albinsson, B.; Moore, T. A.; Moore, A. L; and
Gust D. Molecular AND Logic Gate Based on Electric Dichroism of a
Photochromic Dihydroindolizine. Angew. Chem. Int. Ed. 44 (2005) 75917594.
19. Pettersson, K.; Kyrychenko, A.; Rönnow, E.; Ljungdahl, T.; Mårtensson, J.;
Albinsson, B. Singlet Energy Transfer in Porphyrin-Based Donor-BridgeAcceptor Systems: The Interaction between Bridge Length and Bridge Energy.
J. Phys. Chem. A 110 (2006) 310-318.
20. Pettersson, K.; Wiberg, J.; Ljungdahl, T.; Mårtensson, J.; Albinsson, B.
Interplay between Barrier Width and Height in Electron Tunneling:
Photoinduced Electron Transfer in Porphyrin-Based Donor-Bridge-Acceptor
Systems. J. Phys. Chem. A 110 (2006) 319-326.
21. Eng, M.; Ljungdal, T.; Mårtensson, J; Albinsson, B. Triplet Excitation Energy
Transfer in Donor-Bridge-Acceptor Systems with Conjugated Bridges of
Varying Length. J. Phys. Chem. B 110 (2006) 6483-6491.
22. Ljungdal, T.; Pettersson, K.; Albinsson, B.; Mårtensson, J. Geometrically
Homogenous Series of Covalently Linked Zinc- Free Base
Porphyrin Dimers of Varying Length; Design, Synthesis, and Excitation
Energy Transfer Studies. Eur. J. Org. Chem. (2006) 3087-3096.
23. (*) Eng, M.; Albinsson, B. Non-exponential distance dependence of the
donor-acceptor exchange coupling through π-conjugated molecular bridges.
Angew. Chem. Int. Ed. 45 (2006) 5626-5629.
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Appendix C
24. Andréasson, J.; Straight, S. D.; Albinsson, B.; Moore, T. A; Moore, A. L;
Gust D. All-Photonic Molecular Half-Adder J. Am. Chem. Soc. 128 (2006)
16259-16265.
25. Ljungdal, T.; Pettersson, K.; Albinsson, B.; Mårtensson, J. Solvent and Base
Dependence of Copper Free Palladium Catalyzed Cross-Couplings Between
Terminal Alkynes and Aryliodides; Development of Efficient Conditions for
Construction of Gold(III)/Free Base Porphyrin Dimers. J. Org. Chem. 71
(2006) 1677-1687.
26. Tumpane, J.; Sandin, P.; Kumar, R.; Powers, V. E. C.; Bagglioni, P.; Lehn, J.M.; Albinsson, B.; Lincoln, P.; Wilhelmsson, L. M.; Brown, T.; Nordén, B.
Addressable High-Information-Density DNA Nano-Network Chem. Phys. Lett.
440 (2007) 125-129.
27. Wiberg, J.; Guo, L. J.; Pettersson, K.; Nilsson, D.; Ljungdahl, T.; Mårtensson,
J.; Albinsson, B.. Charge recombination versus charge separation in donorbridge-acceptor systems. J. Am. Chem. Soc.129 (2007) 155-163.
28. Winters, M. U.; Dahlstedt, E.; Blades, H. E.; Wilson, C. J.; Frampton, M. J.;
Anderson, H. L.; Albinsson, B. Electron Transfer through Molecular Wires
Based on Conjugated Porphyrin Oligomers. J. Am. Chem. Soc. 129 (2007)
4291-4297.
29. Nilsson, D.; Watcharinyanon, S.; Eng, M.; Li, L.; Moons, E.; Johansson, L. S.
O.; Zharnikov, M.; Shaporenko, A.; Albinsson, B; Mårtensson, J.
Characterization of Self-Assembled Monolayers of
Oligo(phenyleneethynylene) Derivatives of Varying Shaps on Gold: the Effect
of Laterally Extended π-Systems. Langmuir 23 (2007) 6170-6181.
30. Winters, M. U.; Kärnbrandt, J.; Eng, M.; Wilson, C. J.; Anderson, H. L.;
Albinsson, B. Photophysics of a Butadiyne-Linked Porphyrin Dimer:
Influence of Conformational Flexibility in the Ground and First Singlet
Excited State. J. Phys. Chem. C 111 (2007) 7192-7199.
31. Monhaphol, T.; Albinsson, B.; Wanichwecharungruang, S. 2-Ethylhexyl2,4,5-trimethoxycinnamate and di-(2-ethylhexyl)-2,4,5trimethoxybenzalmalonate as novel UVA filters. J Pharm Pharmacol. 59
(2007) 279-288.
32. Kuimova, M. K.; Hoffmann, M.; Winters, M. U.; Eng, M.; Balaz, M.; Clark, I.
P.; Collins, H. A.; Tavender, S. M.; Wilson, C. J.; Albinsson, B.; Anderson,
H. L.; Parker A. W.; Phillips, D. Determination of the triplet state energies of
a series of conjugated porphyrin oligomers. Photochem. Photobiol. Sci. 6
(2007) 675-682.
33. Winters, M. U.; Kärnbratt, J.; Blades, H. E.; Wilson, C. J.; Frampton, M. J.;
Anderson, H. L.; Albinsson, B. Control of Electron Transfer in a Conjugated
Porphyrin Dimer by Selective Excitation of Planar and Perpendicular
Conformers. Chem. Eur. J. 13 (2007) 7385-7394.
34. Esbjörner , E. K.; Caesar, C. E. B.; Albinsson, B.; Lincoln, P.; Nordén, B.
Tryptophan orientation in model lipid membranes. Biochem. Biopohys. Res.
Comm. 361 (2007) 645–650.
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Appendix C
35. Stengel , G.; Gill, J. P.; Sandin, P.; Wilhelmsson, M.; Albinsson, B.; Nordén,
B.; Millar, D Conformational Dynamics of DNA Polymerase Probed with a
Novel Fluorescent DNA Base Analog Accepted by Biochemistry (2007).
36. Tumpane, J.; Kumar, R.; Lundberg, E., P.; Sandin, P.; Gale, N.,
Nandhakumar, I. S.; Albinsson, B.; Lincoln, P.; Wilhelmsson, L. M.; Brown,
T.; Nordén, B. Triplex Recognition as a Basis for Addressable DNA
Nanostructure Devices Accepted by Nano Letters (2007).
37. Sandin, P., Börjesson, K.; Li, H.; Brown, T.; Wilhelmsson, L. M.; Albinsson,
B. Characterization and Use of an Unprecedentedly Bright and Structurally
Non-perturbing Fluorescent DNA Base Analog. Accepted by Nucl. Acids Res.
(2007).
38. Eng, M. P.; Mårtensson, J.; Albinsson, B. Temperature Dependence of the
Electronic Coupling through OPE-Bridges Accepted by Chem. Eur. J. (2007)
39. Sandin, P.; Wilhelmsson, L. M.; Brown, T.; Lincoln, P.; Albinsson, B.
Dynamics of a 4 nm DNA-Hexagon Studied by Time-Resolved FRET.
Manuscript in preparation.
40. Watcharinyanon, S.; Nilsson, D.; Moons, E.; Zharnikov, M.; Shaporenko, A.;
Albinsson, B; Mårtensson, J.; Johansson, L. S. O. HRXPS and NEXAFS Study
of Self-Assembled Monolayers of Porphyrin Functionalized
Oligo(phenyleneethynylene) on Gold: The Influence of the Binding Group.
Manuscript in preparation.
41. Nilsson, D.; Watcharinyanon, S.; Moons, E.; Johansson, L. S. O.; Zharnikov,
M.; Shaporenko, A.; Albinsson, B; Mårtensson, J. Characterization of SelfAssembled Monolayers of Oligo(phenyleneethynylene) Molecular Wires
Attached to Gold via a Trimethylsilane Anchor Group. Manuscript in
preparation.
42. Kilså, K; Kyrychenko, A.; Larsson, S.; Albinsson, B. Mediated Singlet
Energy Transfer: Temperature Dependence and Theoretical Modelling.
Manuscript in preparation.
43. Pettersson, K.; Kilså, K.; Wasielewski, M. R.; Mårtensson, J.; Albinsson, B.
Ultrafast energy transfer in strongly coupled donor-acceptor systems.
Manuscript in preparation.
44. Wiberg, J; Blum Szuchmacher, A.; Nilsson, D.; Mårtenson, J.; Albinsson, B.
Tunneling Barrier Height Dependence of the Electronic Conduction through
Single π-Conjugated Molecular Wires. Manuscript in preparation.
Books and Review articles.
1. Albinsson, B. and Mårtensson, J. Controlling excitation energy and electron
transfer by tuning the electronic coupling in Energy Harvesting Materials, Ed.
David Andrews, World Scientific Publishing Company Pte Ltd, Singapore
(2005) 187-218.
2. Albinsson B., Eng. M., Pettersson, K. Winters, M. Electron and Energy
Transfer in Donor-Acceptor Systems with Conjugated Molecular Bridges.
Invited article (“Perspective”) to Phys. Chem. Chem. Phys.,
DOI:10.1039/B706122F, in press 2007.
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Appendix C
3. Albinsson, B.; Mårtensson, J. Long-range electron and excitation energy
transfer in designed donor-bridge-acceptor systems. Invited review to J
Photochem. Photobiol., C. (2007) Submitted.
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Appendix C
Publications Mats Andersson,
2003-2007 + important (*).
Peer-reviewed article:
1.
Photophysics of thiophene based polymers in solution: The role of nonradiative decay
processes
J.S. de Melo, H.D. Burrows, M. Svensson, M.R. Andersson, A.P. Monkman
Journal of Chemical Physics 118 (3), (2003), 1550.
2.
A Soluble Donor-Acceptor Double-Cable Polymer: Polythiophene with Pendant
Fullerenes
A. Cravino, G. Zerza, M. Maggini, S. Bucella, M. Svensson, M.R. Andersson, H.
Neugebauer, C.J. Brabec, N.S. Sariciftci
Monatshefte füer Chemie-Chemical Monthly 134 (4), (2003), 519.
3.
Electrochemical bandgaps of substituted polythiophenes
Johansson T, Mammo W, Svensson M, Andersson MR, Inganas O
Journal of Materials Chemistry 13 (6), (2003), 1316.
4. * High-performance polymer solar cells of an alternating polyfluorene copolymer
and a fullerene derivative
Svensson M, Zhang FL, Veenstra SC, Verhees WJH, Hummelen JC, Kroon JM,
Inganas O, Andersson MR
Advanced Materials 15 (12), (2003), 988
5.*
Photodiodes and solar cells based on the n-type polymer poly(pyridopyrazine
vinylene) as electron acceptor
Zhang FL, Jonforsen M, Johansson DM, Andersson MR, Inganas O
Synthetic Metals 138 (3), (2003), 555.
6.
Light amplification in polymer field effect transistor structures
Pauchard M, Swensen J, Moses D, Heeger AJ, Perzon E, Andersson MR
Journal of Applied Physics 94(5), (2003), 3543.
7.
Optical amplification of the cutoff mode in planar asymmetric polymer
waveguides
Pauchard M, Vehse M, Swensen J, Moses D, Heeger AJ, Perzon E, Andersson
MR
Applied Physics Letters 83(22), (2003), 4488.
8.
Photovoltaic devices based on photo induced charge transfer in polythiophene : CN-PPV
blends
Roman LS, Arias AC, Theander M, Andersson MR, Inganas O
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Appendix C
Brazilian Journal of Physics 33 (2), (2003) 376.
9.
Polyfluorene copolymer based bulk heterojunction solar cells
Yohannes T, Zhang F, Svensson A, Hummelen JC, Andersson MR, Inganas O
Thin Solid Films 449(1-2), (2004), 152.
10.
Correlation between oxidation potential and open-circuit voltage of composite solar cells
based on blends of polythiophenes/fullerene derivative
Gadisa A, Svensson M, Andersson MR, Inganas O
Applied Physics Letters 84(9), (2004), 1609.
11.
1 micron wavelength photo- and electroluminescence from a conjugated polymer
Chen M, Perzon E, Andersson MR, Marcinkevicius S, Jönsson SKM, Fahlman M,
Berggren M
Applied Physics Letters 84(18), (2004), 3570.
12.
Influence of buffer layers on the performance of polymer solar cells
Zhang FL, Gadisa A, Inganas O, Svensson M, Andersson MR
Applied Physics Letters 84(19), (2004), 3906.
13.
Electrophosphorescence from substituted poly(thiophene) doped with iridium or platinum
complex
Wang XJ, Andersson MR, Thompson ME, Ingandas O
Thin Solid Films 468 (1-2), (2004), 226.
14.
Infrared photocurrent spectral response from plastic solar cell with low-band-gap
polyfluorene and fullerene derivative
Wang XJ, Perzon E, Delgado JL, de la Cruz P, Zhang FL, Langa F, Andersson M,
Inganas O
Applied Physics Letters 85(21), (2004), 5081.
15.
The electronic states of polyfluorene copolymers with alternating donor-acceptor units
Jespersen KG, Beenken WJD, Zaushitsyn Y, Yartsev A, Andersson M, Pullerits T, and
Sundstrom V.
Journal of Chemical Physics 121(24), (2004), 12613.
16. * Polymer solar cells based on a low band-gap fluorene copolymer and a fullerene
derivative with photocurrent extended to 850 nm.
Zhang F, Perzon E, Wang X, Mammo W, Andersson M R and Inganäs O.
Advanced Functional Materials, 15(5), (2005), 745.
17.
Enhanced photocurrent spectral response in low-bandgap polyfluorene and C70-derivative
based solar cells.
Wang X, Perzon E, Oswald F, Langa F, Admassie S, Andersson M R and Inganäs O.
Advanced Functional Materials, 15(10), (2005), 1665.
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Appendix C
18.
High carrier mobility in low band gap polymer-based field-effect transistors.
Chen, Miaoxiang; Crispin, Xavier; Perzon, Erik; Andersson, Mats R.; Pullerits, Tonu;
Andersson, Mattias; Inganas, Olle; Berggren, Magnus.
Applied Physics Letters 87(25), (2005), 252105/1-252105/3.
19.
Chromic transitions in phenyl-substituted polythiophenes.
Admassie, Shimelis; Mammo, Wendimagegn; Solomon, Theodros; Yohannes, Teketel;
Andersson, Mats R.
Bulletin of the Chemical Society of Ethiopia 19(2), (2005), 267-276.
20.
Multilayer formation in spin-coated thin films of low-bandgap polyfluorene: PCBM
blends.
Bjoerstroem, Cecilia M.; Bernasik, Andrzej; Rysz, Jakub; Budkowski, Andrzej; Nilsson,
Svante; Svensson, Mattias; Andersson, Mats R.; Magnusson, Kjell O.; Moons, Ellen.
Journal of Physics: Condensed Matter 17(50), (2005) L529-L534.
21.
A polymer photodiode using vapour-phase polymerized PEDOT as an anode.
Admassie, Shimelis; Zhang, Fengling; Manoj, A. G.; Svensson, Mattias; Andersson,
Mats R.; Inganaes, Olle.
Solar Energy Materials & Solar Cells 90(2), (2006), 133-141.
22.
Photoinduced absorption in an alternating polyfluorene copolymer for photovoltaic
applications.
Aarnio, H.; Westerling, M.; Oesterbacka, R.; Svensson, M.; Andersson, M. R.; Stubb, H.
Chemical Physics 321(1-2), (2006), 127-132.
23.
Influence of solvent mixing on the morphology and performance of solar cells based on
polyfluorene copolymer/fullerene blends.
Zhang, Fengling; Jespersen, Kim G.; Bjoerstroem, Cecilia; Svensson, Mattias;
Andersson, Mats R.; Sundstroem, Villy; Magnusson, Kjell; Moons, Ellen; Yartsev,
Arkady; Inganaes, Olle.
Advanced Functional Materials 16(5), (2006) 667-674.
24.
Theoretical models and experimental results on the temperature dependence of
polyfluorene solar cells.
Zhang, Fengling; Lacic, Sasa; Svensson, Mattias; Andersson, Mats R.; Inganaes, Olle.
Solar Energy Materials & Solar Cells 90(11), (2006), 1607-1614.
25.
Stoichiometry dependence of charge transport in polymer/methanofullerene and
polymer/C70 derivative based solar cells.
Gadisa, Abay; Wang, Xiangjun; Admassie, Shimelis; Perzon, Erik; Oswald, Frederic;
Langa, Fernando; Andersson, Mats R.; Inganaes, Olle
Organic Electronics (2006), 7(4), 195-204.
26. * Polymer solar cells with low-bandgap polymers blended with C70-derivative give
photocurrent at 1 µm.
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Appendix C
Wang, Xiangjun; Perzon, Erik; Mammo, Wendimagegn; Oswald, Frederic; Admassie,
Shimelis; Persson, Nils-Krister; Langa, Fernando; Andersson, Mats R.; Inganaes, Olle.
Thin Solid Films 511-512 (2006), 576-580.
27.
Electrochemical and optical studies of the band gaps of alternating polyfluorene
copolymers.
Admassie, Shimelis; Inganaes, Olle; Mammo, Wendimagegn; Perzon, Erik; Andersson,
Mats R..
Synthetic Metals 156(7-8), (2006), 614-623.
28. * An alternating low band-gap polyfluorene for optoelectronic devices.
Perzon, Erik; Wang, Xiangjun; Admassie, Shielis; Inganaes, Olle; Andersson, Mats R
Polymer 47(12), (2006), 4261-4268.
29. * Photoinduced Charge Transfer and Efficient Solar Energy Conversion in a Blend of a
Red Polyfluorene Copolymer with CdSe Nanoparticles.
Wang, Peng; Abrusci, Agnese; Wong, Henry M. P.; Svensson, Mattias; Andersson, Mats
R.; Greenham, Neil C
Nano Letters 6(8), (2006), 1789-1793.
30. * Low-bandgap alternating fluorene copolymer/methanofullerene heterojunctions in
efficient near-infrared polymer solar cells.
Zhang, Fengling; Mammo, Wendimagegn; Andersson, Lars M.; Admassie, Shimelis;
Andersson, Mats R.; Inganaes, Olle.
Advanced Materials 18(16), (2006), 2169-2173.
31.
Transparent polymer cathode for organic photovoltaic devices.
Gadisa, Abay; Tvingstedt, Kristofer; Admassie, Shimelis; Lindell, L.; Crispin, X.;
Andersson, Mats R.; Salaneck, W. R.; Inganaes, Olle.
Synthetic Metals 156(16-17), (2006), 1102-1107.
32.
Blue light-emitting diodes based on novel polyfluorene copolymers.
Lindgren, Lars J.; Zhang, Fengling; Admassie, Shimelis; Wang, Xiangjun; Andersson,
Mats R.; Inganaes, Olle.
Journal of Luminescence 122-123 (2007), 610-613.
33.
Evaluation of active materials designed for use in printable electrochromic polymer
displays.
Tehrani, Payman; Isaksson, Joakim; Mammo, Wendimagegn; Andersson, Mats R.;
Robinson, Nathaniel D.; Berggren, Magnus
Thin Solid Films 515(4), (2006), 2485-2492.
34.
Improvements of fill factor in solar cells based on blends of polyfluorene copolymers as
electron donors.
Gadisa, Abay; Zhang, Fengling; Sharma, Deepak; Svensson, Mattias; Andersson, Mats
R.; Inganaes, Olle.
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Appendix C
Thin Solid Films 515(5), (2007), 3126-3131.
35. * Donor and Acceptor Behavior in a Polyfluorene for Photovoltaics.
Wong, Henry M. P.; Wang, Peng; Abrusci, Agnese; Svensson, Mattias; Andersson, Mats
R.; Greenham, Neil C.
Journal of Physical Chemistry C 111(13), (2007), 5244-5249.
36.
Red and near infrared polarized light emissions from polyfluorene copolymer based light
emitting diodes.
Gadisa, Abay, Perzon, Erik, Andersson, Mats R., Inganas, Olle
Applied Physics Letters 90(11), (2007), 113510
Peer- reviewed conference contributions:
1.
Synthesis and properties of alternating polyfluorene copolymers with redshifted
absorption for use in solar cells.
M. Svensson, F. Zhang, O. Inganas, M.R. Andersson
Synthetic Metals 135 (1-3), (2003) 137.
2.
Conformational transitions in a free amino acid functionalized polythiophene.
K.P.R. Nilsson, M.R. Andersson, O. Inganas.
Synthetic Metals 135 (1-3), (2003), 291.
3.
Electrophosphorescence from polythiophene blends light-emitting diodes.
X.J. Wang, M. Andersson, M.E. Thompson, O. Inganas
Synthetic Metals 137 (1-3), (2003), 1019.
4.
Polymer solar cells based on MEH-PPV and PCBM.
F.L. Zhang, M. Johansson, M.R. Andersson, J.C: Hummelen, O. Inganas
Synthetic Metals 137 (1-3), (2003), 1401.
5.
Low band gap donor-acceptor-donor polymers for infra-red electroluminescence and
transistors
Chen MX, Perzon E, Robisson N, Jonsson SKM, Andersson MR, Fahlman M, Berggren
M
Synthetic Metals 146(3), (2004), 233.
6.
Design, synthesis and properties of low band gap polyfluorenes for photovoltaic devices.
Perzon E, Wang X, Zhang F, Mammo W, Delgado J L, De la Cruz P, Inganäs O, Langa F
and Andersson M R.
Synthetic Metals, 154, (2005), 53.
7.
Synthesis and properties of polyfluorenes with phenyl substituents
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Appendix C
L. J. Lindgren, X. Wang, O. Inganäs, M. R. Andersson
Synthetic Metals, 154, (2005), 97.
8.
Recombination studies in a polyfluorene copolymer for photovoltaic applications.
Aarnio, Harri; Westerling, Markus; Oesterbacka, Ronald; Svensson, Mattias; Andersson,
Mats R.; Pascher, Torbjoern; Pan, Jinxi; Sundstroem, Villy; Stubb, Henrik
Synthetic Metals 155(2), (2005), 299-302.
9.
Influence of solvents and substrates on the morphology and the performance of lowbandgap polyfluorene: PCBM photovoltaic devices.
Bjoerstroem, Cecilia M.; Nilsson, Svante; Magnusson, Kjell O.; Moons, Ellen; Bernasik,
Andrzej; Rysz, Jakub; Budkowski, Andrzej; Zhang, Fengling; Inganaes, Olle; Andersson,
Mats R.. Proceedings of SPIE-The International Society for Optical Engineering
(2006), 6192(Organic Optoelectronics and Photonics II), 61921X/1-61921X/9.
Review articles, book chapters, books
1. * Low bandgap alternating polyfluorene copolymers in plastic photodiodes and solar cells
Inganas O, Svensson M, Zhang F, Gadisa A, Persson NK, Wang X, Andersson MR
Applied Physics A-Materials Science & Processing 79(1), (2004), 31.
2. * Alternating fluorene copolymer–fullerene blend solar cells
O. Inganäs, F. Zhang, X. Wang, A. Gadisa, N. K. Persson, M. Svensson, E. Perzon, W.
Mammo, and M. R. Andersson
Organic Photovoltaics: Mechanisms, Materials and Devices, Editor Sun and Sariciftci,
CRC Press, Boca Raton, Fl. USA, (2005), 387-402.
Popular-scientific articles/presentations
•
•
Seminar for the public, Palmtedtsalen, Chalmers, April 10, 2003, “Värme och
el från solen; Solceller av plast”
Seminar for teachers ”Technology for teachers” October 21, 2004, ”Teknik
och Vardag; TV-skärmar och solceller av plast!?“
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Appendix C
Publications Linda Gunnarsson
1. E.M. Hicks, S. Zou, G.C. Schatz, K.G. Spears, R.P. Van Duyne, L.
Gunnarsson, T. Rindzevicius, B. Kasemo, and M. Käll "Controlling plasmon line-shapes
through diffractive coupling in linear arrays of cylindrical nanoparticles fabricated by electron
beam lithography." Nano Letters 5, 1065-1070 (2005). (17 citations)
2. L. Gunnarsson, T. Rindzevicius, J. Prikulis, B. Kasemo, S. Chou, G. C. Schatz and M.
Käll, ”Confined plasmons in nanofabricated single silver particle pairs-Experimental
observations of strong interparticle interactions. ”, Journal of Physical Chemistry B, 2005, 19,
1079-1087. (32 citations)
3. C. L. Haynes, L. Gunnarsson, A. D. McFarland, L Zhao, G. Schatz, R. P. Van Duyne, J.
Prikulis, B. Kasemo, and M. Käll , “Nanoparticle Optics: The importance of radiative dipole
coupling in two dimensional nanoparticle arrays”, Journal of Physical Chemistry B,
2003,107, 7337-7342 (87 citations)
4. J. Prikulis, H. Xu, L. Gunnarsson, H. Olin, and M. Käll, ”Phase-sensitive near-field
imaging of metal nanoparticles”, J. Appl. Phys.92, 6211, 2002. (10 citations)
5. Gunnarsson, L., E. J. Bjerneld, S. Petronis, H. Xu, B. Kasemo, and M. Käll, ”Interparticle
Coupling Effects in Nanofabricated Substrates for Surface Enhanced Raman Scattering”,
Applied Physics Letters, 2001. 78: p. 802-804. (45 citations)
6. S. H. M. Persson, L. Olofsson, and L. Gunnarsson, ”A Self-Assembled Single-Electron
Tunneling Transistor”, Applied Physics Letters, 74, 2546-2548 (1999) (40 citations)
19
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Appendix C
Publications Krister Holmberg
10 selected publications
K. Holmberg and B. Hansen. Ester Synthesis with Dicyclohexyl-carbodiimide Improved by
Acid Catalysts. Acta Chem. Scand. B33 (1979) 410.
M.-B. Stark and K. Holmberg. Covalent Immobilization of Lipase in Organic Solvents.
Biotechn. Bioeng. 34 (1989) 942.
K. Holmberg. Polymerizable Surfactants. Progr. Org. Coatings 20 (1992) 325.
Österberg, K. Bergström, K. Holmberg, J.A. Riggs, J.M. Van Alstine, T.P. Schuman, N.L.
Burns and J.M. Harris. Comparison of Polysaccharide and Poly(ethylene Glycol) Coatings for
Reduction of Protein Adsorption on Polystyrene Surfaces. Colloids Surfaces A 77 (1993) 159.
K. Holmberg. Organic and Bioorganic Reactions in Microemulsions. Adv. Colloid Interface Sci.
51 (1994) 137.
M. Malmsten, B. Lassen, K. Holmberg, V. Thomas and G. Quash. Effects of Hydrophilization
on the Interfacial Behaviour of Immunoglobulins. J. Colloid Interface Sci. 177 (1996) 70.
E. Alami and K. Holmberg. Heterogemini Surfactants based on Fatty Acid; Synthesis and
Interfacial Properties. J. Colloid Interface Sci. 239 (2001) 230.
H. Härelind Ingelsten, J.-C. Beziat, K. Bergkvist, A. Palmqvist, M. Skoglundh, H. Qiuhong,
L.K.L. Falk and K. Holmberg. Deposition of Platinum Nanoparticles, Synthesized in Water-inOil Microemulsions, on Alumina Supports. Langmuir 18 (2002) 1811.
K. Holmberg. Surfactants-Templated Nanomaterials Synthesis. J. Colloid Interface Sci. 274
(2004) 355.
M. Häger and K. Holmberg. Phase Transfer Agents as Catalysts for a Nucleophilic Substitution
Reaction in Microemulsions. Chem. Eur. J. 10 (2004) 5460.
2003-2007
Peer reviewed articles
F. Currie, G. Westman and K. Holmberg. Bromination in Microemulsion. Colloids
Surfaces A 215 (2003) 51.
M. Yashima, L.K.L. Falk, A.E.C. Palmqvist and K. Holmberg. Structure and Catalytic
Properties of Nano-sized Alumina Supported Platinum and Palladium Particles
Synthesized by Reaction in Microemulsion. J. Colloid Interface Sci. 268 (2003) 348.
20
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Appendix C
A. K. Morén, A. Kumar, G. Stenhagen and K. Holmberg. Synthesis of a Macrocyclic
Lactone: 1. Reaction in Homogeneous Media. J. Disp. Sci. Technol. 24 (2003) 79.
A. Kumar, A. K. Morén and K. Holmberg. Synthesis of a Macrocyclic Lactone: 2.
Reaction in Microheterogeneous Media. J. Disp. Sci. Technol. 24 (2003) 89.
O.P. Yadav, A. Palmqvist, N. Cruise and K. Holmberg. Synthesis of Platinum
Nanoparticles in Microemulsions and their Catalytic Activity for the Oxidation of
Carbon Monoxide. Colloids Surfaces A 221 (2003) 131.
K. Mohlin, K. Holmberg, J. Esquena and C. Solans. Study of Low Energy
Emulsification of Alkyl Ketene Dimer (AKD) Related to the Phase Behaviour of the
System. Colloids Surfaces A 218 (2003) 189.
G. Kickelbick, J. Bauer, N. Huesing, M. Andersson and K. Holmberg. The Aggregation
Behavior of Short-Chain PDMS-b-PEO Diblock Copolymers in Aqueous Solutions.
Langmuir 19 (2003) 10073.
A. Kumar, E. Alami, K. Holmberg, V. Seredyuk and F.M. Menger. Branched
Zwitterionic Gemini Surfactants: Micellization and Interaction with Ionic Surfactants.
Colloids Surfaces A 228 (2003) 197.
M. Stjerndahl and K. Holmberg. Synthesis and Chemical Hydrolysis of Surface Active
Esters. J. Surfact. Deterg. 6 (2003) 311.
M. Stjerndahl, C. G van Ginkel and K. Holmberg. Hydrolysis and Biodegradation
Studies of Surface Active Esters. J. Surf. Deterg. 6 (2003) 319.
K. Holmberg. Surfactants-Templated Nanomaterials Synthesis. J. Colloid Interface Sci.
274 (2004) 355.
D. Lundberg and K. Holmberg. NMR Studies on Hydrolysis Kinetics and Micellar
Growth in Solutions of Surface Active Betaine Esters. J. Surfact. Deterg. 7 (2004) 239.
C. Groth, M. Nydén, K. Holmberg, J.R. Kanicky and D.O. Shah. Kinetics of SelfAssembly of Gemini Surfactants. J. Surfact. Deterg. 7 (2004) 247.
M. Dahlström, P.R. Jonsson, J. Lausmaa, T. Arnebrant, M. Sjögren, K. Holmberg,
L.G.E. Mårtensson and H. Elwing. The impact of polymer surface affinity of novel
antifouling agents. Biotech. Bioeng. 86 (2004) 1.
J.-A. Östlund, H. S. Fogler, M. Nydén and K. Holmberg. Functional Groups in
Fractionated Asphaltenes and the Adsorption of Amphiphilic Molecules. Colloids
Surfaces A 234 (2004) 95.
M. Häger, U. Olsson and K. Holmberg. A Nucleophilic Substitution Performed in
Different Types of Self-assembly Structures. Langmuir. 20 (2004) 6107.
21
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Appendix C
M. Häger, F. Currie and K. Holmberg. A Nucleophilic Substitution Reaction in
Microemulsions Based on either an Alcohol Ethoxylate or a Sugar Surfactant. Colloids
Surfaces A. 250 (2004) 163.
M. Häger and K. Holmberg. Phase Transfer Agents as Catalysts for a Nucleophilic
Substitution Reaction in Microemulsions. Chem. Eur. J. 10 (2004) 5460.
D. Lundberg, H. Ljusberg-Wahren, A. Norlin and K. Holmberg. Studies on Dodecyl
Betainate in Combination with its Degradation Products or with Phosphatidyl Choline. J.
Colloid Interface Sci. 278 (2004) 478..
F. Currie, M. Andersson and K. Holmberg. Oxidation of Self-organized Nonionic
Surfactants. Langmuir 20 (2004) 3835.
L.S. Shtykova, D. Ostrovskii, P. Handa, K. Holmberg and M. Nydén. NMR
Diffusometry and FTIR in the Study of the Interaction between Antifouling Agent and
Binder in Marine Paints. Progr. Org. Coating. 51 (2004) 125.
Y.I Rabinovich, J.R. Kanicky, S. Pandey, H. Oskarsson, K. Holmberg, B.M. Moudgil
and D.O. Shah. Strength of Self-Assembled Gemini Surfactant Films and Dispersion
Stability. J. Colloid Interface Sci. 288 (2005) 583.
T. Witula and K. Holmberg. Use of a Mesoporous Material for Organic Synthesis.
Langmuir 21 (2005) 3782.
A. Nilsson, C. Fant, M. Nydén and K. Holmberg. Lipopolysaccharide Removal by a
Peptide-Functionalized Surface. Colloids Surfaces B 40 (2005) 73.
G. Kickelbick, J. Bauer, N. Huesing, M. Andersson and K. Holmberg. The Binary Phase
Behavior of Short-Chain PDMS-b-PEO Diblock Copolymers in Aqueous Solutions.
Colloids Surfaces A 254 (2005) 37.
M. Stjerndahl and K. Holmberg. Synthesis and Stability Studies of a Surface-Active
Amide. J. Surfact. Deterg. 8 (2005) 1.
M. Stjerndahl and K. Holmberg. Hydrolyzable Nonionic Surfactants. Stability and
Physicochemical Properties of Surfactants Containing Carbonate, Ester and Amide
Bonds. J. Colloid Interface Sci. 291 (2005) 570.
D. Lundberg, M. Stjerndahl and K. Holmberg. Mixed Micellar Systems of Cleavable
Surfactants. Langmuir 21 (2005) 8658.
L. Palmqvist, O. Lyckfeldt, E. Carlström, P. Davoust, A. Kauppi and K. Holmberg.
Dispersion Mechanism in Aqueous Alumina Suspensions at High Solids Content.
Colloids Surfaces A 274 (2006) 100.
K. Mohlin, P. Karlsson and K. Holmberg. Use of Cleavable Surfactants for Alkyl
Ketene Dimer (AKD) Dispersions. Colloids Surfaces A 274 (2006) 200.
22
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Appendix C
K. Wikander, P. Kjellin and K. Holmberg. Use of Lignin as a Calcium Carbonate
Scaling Inhibitor. Nordic Pulp Paper Sci. J. 21 (2006) 286 .
K. Wikander, A.E.C. Palmqvist, K. Holmberg, H. Ekström, A. Lundblad and G.
Lindbergh. Alternative Catalysts and Carbon Support Materials for PEMFC. Fuel Cells
6 (2006) 21.
K. Mohlin and K. Holmberg. Nonionic Ortho Ester Surfactants as Cleavable
Emulsifiers. J. Colloid Interface Sci. 299 (2006) 435.
H. Oskarsson and K. Holmberg. Adsorption of Ethoxylated Cationic Surfactants on
Self-assembled Monolayers of Alkanethiols on Gold Using Surface Plasmon Resonance
Detection. J. Colloid Interface Sci. 301 (2006) 360.
K. Wikander, C. Petit, K. Holmberg and M.-P. Pileni. Size Control and Growth Process
of Alkylamine–Stabilized Platinum Nanocrystals: A Comparison between the Phase
Transfer and Reverse Micelles Methods. Langmuir 22 (2006) 4863.
P. Reis, K. Holmberg, T. Debeche, B. Folmer, L. Fauconnot and H. Watzke. Lipase
Catalyzed Reactions at Different Surfaces. Langmuir 22 (2006) 8169.
K. Wikander, A.B. Hungria, P.A. Midgley, A.E.C. Palmqvist, K. Holmberg and J. M.
Thomas. Incorporation of Platinum Nanoparticles in Ordered Mesoporous Carbon. J
Colloid Interface Sci. 305 (2007) 204.
T. Witula and K. Holmberg. Liquid Crystalline Phases and other Microheterogeneous
Systems as Media for Organic Synthesis. J. Disp. Sci. Technol. 28 (2007) 1. *
P. Handa, M. Stjerndahl and K. Holmberg. A Carbon-Carbon Coupling Reaction
Catalyzed by a Water Soluble Rhodium Catalyst Entrapped in Mesoporous Silica.
Micropor. Mesopor. Mater. 100 (2007) 146. *
H. Oskarsson, M. Frankenberg, A. Annerling and K. Holmberg. Adsorption of Novel
Alkylaminoamide Sugar Surfactants at Tailor-Made Surfaces. J. Surfact. Deterg. 10
(2007) 41.
T. Witula and K. Holmberg. Use of different types of mesoporous materials as tools for
organic synthesis. J. Colloid Interface Sci. 310 (2007) 536. *
F. Currie, P. Jarvoll, K. Holmberg and L.S. Romsted. Regioselectivity of a Substitution
Reaction in a Micellar System. J. Colloid Interface Sci. 312 (2007) 453. *
A.R. Tehrani-Bagha, H. Oskarsson, C.G. van Ginkel and K. Holmberg. Cleavable
Cationic Gemini Surfactants. 1. Chemical Hydrolysis and Biodegradation. J. Colloid
Interface Sci. 312 (2007) 444.
P. Reis, T. Witula and K. Holmberg. Mesoporous Materials as Host for an Entrapped
Enzyme. Micropor. Mesopor. Mater. (2007) In press. *
23
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Appendix C
A. Bergstrand, I. Ceylan, G. Quash, M. Nydén and K. Holmberg. Towards a Biosensor
Immunoassay of Isopeptide Antigens in Human Plasma. J. Immunol, Meth. In press.
S. Lam, A.C. Hellgren, M. Sjöberg, K. Holmberg, H.A.S. Schoonbrood, J.M. Asua, K.
Tauer, D.C. Sherrington and A. Montoya Goni. Surfactants in Heterophase
Polymerization: A Study of Film Formation using Atomic Force Microscopy, J. Appl.
Polym. Sci. 66 (1997) 187. *
M. Malmsten, B. Lassen, K. Holmberg, V. Thomas and G. Quash. Effects of
Hydrophilization on the Interfacial Behaviour of Immunoglobulins. J. Colloid Interface
Sci. 177 (1996) 70. *
E. Österberg, K. Bergström, K. Holmberg, T.P. Schuman, J.A. Riggs, N.L. Burns, J.M.
Van Alstine and J.M. Harris. Protein-Rejecting Ability of Surface-Bound Dextran in
End-On and Side-On Configurations. Comparison to PEG. J. Biomed. Mater. Res. 29
(1995) 741. *
K. Holmberg. Organic and Bioorganic Reactions in Microemulsions. Adv. Colloid
Interface Sci. 51 (1994) 137. *
K. Bergström, K. Holmberg, A. Safranj, A.S. Hoffman, M. Edgell, B.A. Hovanes and
J.M. Harris. Reduction of Protein Adsorption on PEG-Coated Polystyrene. J. Biomed.
Mater. Res. 26 (1992) 779. *
Peer reviewed conference proceedings
K. Holmberg. Use of Surfactant Self-Assembly for Nanomaterial Synthesis. Proceedings
6th World Surfactant Congress, June 2004, Berlin.
A. Berggren, K. Holmberg and A.E.C. Palmqvist. Synthesis of Stable Colloidal
Suspensions of Ordered Mesostructured Silica from Sodium Metasilicate Using Pluronic
P123 and Mildly Acidic Conditions. Studies in Surface Science and Catalysis:
Proceedings of the 5th International Mesostructured Materials Symposium, Shanghai,
China, August 2006.
Peer reviewed review papers and book chapters
E. Alami and K. Holmberg. Heterogemini Surfactants. Adv. Colloids Interface Sci. 100102 (2003) 13.
K. Holmberg. Organic Reactions in Microemulsions. Curr. Opin. Colloid Interface Sci.
8 (2003) 187.
K. Holmberg and G. Quash. Control of Protein Adsorption in Solid-Phase Diagnostics
and Therapeutics. In: Biopolymers at Interfaces, 2nd ed. (Ed. M. Malmsten), Marcel
Dekker, New York, 2003, pp. 741-771.
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Appendix C
K. Holmberg. Applications of Surfactants in Paints. In: Surfactants in Polymers,
Coatings, Inks and Adhesives (Ed. D.R. Karsa), Blackwell Publ., Oxford, 2003, pp. 152179.
M. Häger, F. Currie and K. Holmberg. Organic Reactions in Microemulsions. In:
Colloid Chemistry II (Ed. M. Antonietti) Topics in Current Chemistry 227, SpringerVerlag, Heidelberg, 2003, pp. 53-74.
M. Stjerndahl, D. Lundberg and K. Holmberg. Cleavable Surfactants. In: Novel
Surfactants, 2nd ed. (Ed. K. Holmberg), Marcel Dekker, New York, 2003, pp. 317-345.
M. Andersson, H. Härelind Ingelsten, A. Palmqvist, M. Skoglundh and K. Holmberg.
Use of Self Assembling Surfactants as Templates and Reactants for the Synthesis of
Noble Metal Particles. In: Self-Assembly (Ed. B. H. Robinson), IOS Press, 2003, pp.
105-111.
K. Holmberg. Organic Reactions in Emulsions and Microemulsions. In: Emulsions and
Emulsion Stability, 2nd ed. (Ed. J. Sjöblom) Marcel Dekker, 2005, pp. 263-282.
A. Berggren, A.E.C. Palmqvist and K. Holmberg. Surfactant-Templated Mesostructured
Materials from Inorganic Silica. Soft Matter 1 (2005) 219.
K. Holmberg. Alkyd Resins. In: Coatings Technology Handbook 3rd ed. (Ed. A.A.
Tracton), Taylor and Francis, Boca Raton, FL, 2006, pp. 51-1 – 51-12.
K. Holmberg. Paints and Printing Inks. In: Handbook of Detergents. Part D:
Formulation (Ed. M.S. Showell), Taylor and Francis, Boca Raton, FL, 2006, pp. 369386.
A. Lif and K. Holmberg. Water-in-diesel Emulsions and Related Systems. Adv. Colloid
Interface Sci. 123-126 (2006) 231.
P. Karlsson, A.E.C. Palmqvist and K. Holmberg. Surface Modification for Aluminium
Pigment Inhibition. Adv. Colloid Interface Sci. 128-130 (2006) 121.
K. Holmberg. Organic Reactions in Microemulsions. Eur. J. Org. Chem. (2007) 731.
D. Lundberg, M. Stjerndahl and K. Holmberg. Surfactants Containing Hydrolyzable
Bonds. Topics in Applied Physics, Springer-Verlag. In press.
M. Andersson, A. Palmqvist and K. Holmberg. Use of Self-Assembled Surfactants for
Nanomaterials Synthesis. In Particulate Systems in Nano and Biotechnologies (Eds. W.
Sigmund, H. El-Shall, B. Moudgil and D.O. Shah), Taylor & Francis, In press.
A. R. Tehrani-Bagha and K. Holmberg. Cleavable Surfactants. Current Opinion Colloid
Interface Sci. 12 (2007) 81.
Books
25
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Appendix C
K. Holmberg (ed.), Novel Surfactants. Synthesis, Applications and Biodegradability, 2nd ed.,
Marcel Dekker, New York, 2003.
Patents
I am coauthor on four patents or patent applications during 2003-2007. The patents have been
transfered to the following companies:
- Eka Chemicals ("Emulgatorer för AKD-dispersioner")
- Camurus ("Läkemedelsformulering med hjälp av ytaktiva betainestrar")
- I-Tech ("Båtbottenfärger med kontrollerad frisättning av påväxtförhindrande substans")
- Appeartex ("Antibakteriella ytor")
Polpular science
M. Nydén och K. Holmberg. Miljövänliga båtbottenfärger. Kemivärlden. Maj 2006.
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Appendix C
Publications Fredrik Höök
2003-2007 + important (*).
7.*
Hook F, Rodahl M, Kasemo B, Brzezinski P: Structural changes in hemoglobin
during adsorption to solid surfaces: Effects of pH, ionic strength, and ligand
binding. Proceedings of the National Academy of Sciences of the United States
of America 1998, 95:12271-12276.
12.* Hook F, Kasemo B, Nylander T, Fant C, Sott K, Elwing H: Variations in
coupled water, viscoelastic properties, and film thickness of a Mefp-1 protein
film during adsorption and cross-linking: A quartz crystal microbalance with
dissipation monitoring, ellipsometry, and surface plasmon resonance study.
Analytical Chemistry 2001, 73:5796-5804.
http://dx.doi.org/10.1021/ac0106501
13.* Hook F, Ray A, Norden B, Kasemo B: Characterization of PNA and DNA
immobilization and subsequent hybridization with DNA using acoustic-shearwave attenuation measurements. Langmuir 2001, 17:8305-8312.
http://dx.doi.org/10.1021/la0107704
21.
Graneli A, Rydstrom J, Kasemo B, Hook F: Formation of supported lipid
bilayer membranes on SiO2 from proteoliposomes containing transmembrane
proteins. Langmuir 2003, 19:842-850.
http://dx.doi.org/10.1021/la026231w
22.
Larsson C, Rodahl M, Hook F: Characterization of DNA immobilization and
subsequent hybridization on a 2D arrangement of streptavidin on a biotinmodified lipid bilayer supported on SiO2. Analytical Chemistry 2003, 75:50805087.
http://dx.doi.org/10.1021/ac034269n
23.
Olofsson L, Rindzevicius T, Pfeiffer I, Kall M, Hook F: Surface-based goldnanoparticle sensor for specific and quantitative DNA hybridization detection.
Langmuir 2003, 19:10414-10419.
http://dx.doi.org/10.1021/la0352927
24.
Otzen DE, Oliveberg M, Hook F: Adsorption of a small protein to a methylterminated hydrophobic surfaces: effect of protein-folding thermodynamics and
kinetics. Colloids and Surfaces B-Biointerfaces 2003, 29:67-73.
http://dx.doi.org/10.1016/S0927-7765(02)00186-8
25.* Reimhult E, Hook F, Kasemo B: Intact vesicle adsorption and supported
biomembrane formation from vesicles in solution: Influence of surface
chemistry, vesicle size, temperature, and osmotic pressure. Langmuir 2003,
19:1681-1691.
http://dx.doi.org/10.1021/la0263920
26.
Svedhem S, Dahlborg D, Ekeroth J, Kelly J, Hook F, Gold J: In situ peptidemodified supported lipid bilayers for controlled cell attachment. Langmuir 2003,
19:6730-6736.
http://dx.doi.org/10.1021/la034172w
27
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Appendix C
27.* Svedhem S, Pfeiffer I, Larsson C, Wingren C, Borrebaeck C, Hook F: Patterns
of DNA-labeled and scFv-antibody-carrying lipid vesicles directed by materialspecific immobilization of DNA and supported lipid bilayer formation on an
Au/SiO2 template. Chembiochem 2003, 4:339-343.
http://dx.doi.org/10.1002/cbic.200390055
28.
Graneli A, Edvardsson M, Hook F: DNA-based formation of a supported, threedimensional lipid vesicle matrix probed by QCM-D and SPR. Chemphyschem
2004, 5:729-733.
http://dx.doi.org/10.1002/cphc.200301061
29.
Graneli A, Rydstrom J, Kasemo B, Hook F: Utilizing adsorbed proteoliposomes
trapped in a non-ruptured state on SiO2 for amplified detection of membrane
proteins. Biosensors & Bioelectronics 2004, 20:498-504.
http://dx.doi.org/10.1016/j.bios.2004.02.013
30.
Limson J, Odunuga OO, Green H, Hook F, Blatch GL: The use of a quartz
crystal microbalance with dissipation for the measurement of protein-protein
interactions: a qualitative and quantitative analysis of the interactions between
molecular chaperones. South African Journal of Science 2004, 100:678-682.
http://www.nrf.ac.za/sajs/index.stm
31.* Pfeiffer I, Hook F: Bivalent cholesterol-based coupling of oligonucletides to
lipid membrane assemblies. Journal of the American Chemical Society 2004,
126:10224-10225.
http://dx.doi.org/10.1021/ja048514b
32.
Reimhult E, Larsson C, Kasemo B, Hook F: Simultaneous surface plasmon
resonance and quartz crystal microbalance with dissipation monitoring
measurements of biomolecular adsorption events involving structural
transformations and variations in coupled water. Analytical Chemistry 2004,
76:7211-7220.
http://dx.doi.org/10.1021/ac0492970
33.
Stadler B, Falconnet D, Pfeiffer I, Hook F, Voros J: Micropatterning of DNAtagged vesicles. Langmuir 2004, 20:11348-11354.
http://dx.doi.org/10.1021/la0482305
34.
Asberg P, Bjork P, Hook F, Inganas O: Hydrogels from a water-soluble
zwitterionic polythiophene: Dynamics under pH change and biomolecular
interactions observed using quartz crystal microbalance with dissipation
monitoring. Langmuir 2005, 21:7292-7298.
http://dx.doi.org/10.1021/la050479e
35.
Benkoski JJ, Hook F: Lateral mobility of tethered vesicle - DNA assemblies.
Journal of Physical Chemistry B 2005, 109:9773-9779.
http://dx.doi.org/10.1021/ac050116j
36.
Benkoski JJ, Jesorka A, Kasemo B, Hook F: Light-activated desorption of
photoactive polyelectrolytes from supported lipid bilayers. Macromolecules
2005, 38:3852-3860.
http://dx.doi.org/10.1021/ma048046q
37.* Dahlin A, Zach M, Rindzevicius T, Kall M, Sutherland DS, Hook F: Localized
surface plasmon resonance sensing of lipid-membrane-mediated biorecognition
28
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Appendix C
events. Journal of the American Chemical Society 2005, 127:5043-5048.
http://dx.doi.org/10.1021/ja043672o
38.
Edvardsson M, Rodahl M, Kasemo B, Hook F: A dual-frequency QCM-D setup
operating at elevated oscillation amplitudes. Analytical Chemistry 2005,
77:4918-4926.
http://dx.doi.org/10.1021/ac050116j
39.
Ghatnekar-Nilsson S, Lindahl J, Dahlin A, Stjernholm T, Jeppesen S, Hook F,
Montelius L: Phospholipid vesicle adsorption measured in situ with resonating
cantilevers in a liquid cell. Nanotechnology 2005, 16:1512-1516.
http://dx.doi.org/10.1088/0957-4484/16/9/017
40.
Larsson C, Bramfeldt H, Wingren C, Borrebaeck C, Hook F: Gravimetric
antigen detection utilizing antibody-modified lipid bilayers. Analytical
Biochemistry 2005, 345:72-80.
http://dx.doi.org/10.1016/j.ab.2005.05.031
41.
Rindzevicius T, Alaverdyan Y, Dahlin A, Hook F, Sutherland DS, Kall M:
Plasmonic sensing characteristics of single nanometric holes. Nano Letters
2005, 5:2335-2339.
http://dx.doi.org/10.1021/nl0516355
42.
Stengel G, Hook F, Knoll W: Viscoelastic modeling of template-directed DNA
synthesis. Analytical Chemistry 2005, 77:3709-3714.
http://dx.doi.org/10.1021/ac048302x
43.
Benkoski JJ, Jesorka A, Edvardsson M, Hook F: Light-regulated release of
liposomes from phospholipid membranes via photoresponsive polymer-DNA
conjugates. Soft Matter 2006, 2:710-715.
http://dx.doi.org/10.1039/b606123k
44.
Dahlin AB, Tegenfeldt JO, Hook F: Improving the instrumental resolution of
sensors based on localized surface plasmon resonance. Analytical Chemistry
2006, 78:4416-4423.
http://dx.doi.org/10.1021/ac0601967
45.
Edvardsson M, Rodahl M, Hook F: Investigation of binding event perturbations
caused by elevated QCM-D oscillation amplitude. Analyst 2006, 131:822-828.
http://dx.doi.org/10.1039/b601800a
46.
Marie R, Beech JP, Voros J, Tegenfeldt JO, Hook F: Use of PLL-g-PEG in
micro-fluidic devices for localizing selective and specific protein binding.
Langmuir 2006, 22:10103-10108.
http://dx.doi.org/10.1021/ac061280p
47.
Pfeiffer I, Hook F: Quantification of oligonucleotide modifications of small
unilamellar lipid vesicles. Analytical Chemistry 2006, 78:7493-7498.
http://dx.doi.org/10.1021/ac061280p
48.
Reimhult E, Zach M, Hook F, Kasemo B: A multitechnique study of liposome
adsorption on Au and lipid bilayer formation on SiO2. Langmuir 2006, 22:33133319.
http://dx.doi.org/10.1021/la0519554
49.
Zhdanov VP, Edvardsson M, Hook F, Kasemo B: Suppression of binding events
via external perturbation with emphasis on QCM. Chemical Physics Letters
29
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Appendix C
2006, 424:214-217.
http://dx.doi.org/10.1016/j.cplett.2006.04.030
50.* Edvardsson M, Zhdanov V, Hook F: Controlled Radial Distribution of
Nanoscale Vesicles During Binding to an Oscillating QCM Surface. Small 2007,
3:585-589.
http://dx.doi.org/10.1002/smll.200600458
51.
Marie R, Dahlin AB, Tegenfeldt JO, Hook F: A generic surface modification
strategy for sensing applications based on Au/SiO2 nanostructures.
Biointerphases 2007, 2: 49.
http://dx.doi.org/10.1116/1.2717926
52.
Zhou Y, Xu H, Dahlin AB, Gustafson J, Borrebaeck CAK, Wingren C, Liedberg
B, Hook F: Quantitative Interpretation of Gold Nanoparticle-Based Bioassays
Designed for Detection of Immuno-Complex Formation. Biointerphases 2007,
2:6.
http://dx.doi.org/10.1116/1.2700235
53.
Granéli A, Benkoski JJ, Hook F: Characterization of a proton pumping
transmembrane protein incorporated in a supported three-dimensional matrix of
proteoliposomes. Analytical Biochemistry 2007, 367 (1): 87-94
http://dx.doi.org/10.1016/j.ab.2007.03.031
54.
Prinz C, Malm J, Hook F, Sjövall P: Structural effects in the analysis of
supported lipid bilayers by time-of-flight secondary ion mass spectrometry
(TOF-SIMS). 2007, Langmuir 23 (15): 8035-8041
http://dx.doi.org/10.1021/la7004634
55.* Stengel G, Zahn R, Hook F: DNA-induced programmable fusion of
phospholipid vesicles. Journal of the American Chemical Society 2007, 129
(31): 9584-9585
http://dx.doi.org/10.1021/ja073200k
56.* Jonsson, M. P.; Jonsson, P.; Dahlin, A. B.; Hook, F.Supported Lipid Bilayer
Formation and Lipid-Membrane-Mediated Biorecognition Reactions Studied
with a New Nanoplasmonic Sensor Template, Nano Letters, 2007; ASAP
Article
http://dx.doi.org/10.1021/nl072006t
57.
Gunnarsson A, Jonsson P, Marie R, Tegenfeld J, Hook F, Single-molecule
Detection of Unlabeled DNA Targets, Nano Letters, revision requested
58.
Dahlin A, Jonsson M, and Hook F: Size and sequence selective coupling of
individual vesicles in nanoplasmonic apertures. Advanced Materials 2007,
accepted
59.
Klenkar G, Brian B, Ederth T, Stengel, G, Hook F, Piehler J, Liedberg B, A
Microarray for Adressable Adsorption of Lipid Vesicles and Subsequent LabelFree Protein Interaction Studies, Biointerphases, (2007) submitted
60.
Zhou Y, Xu H, and Hook F: A nanoplasmonic-based method for refractive
index determinations of adsorbed proteins. Small 2007, in writing
61.
Wang G, Richter, R, Rodahl, M, Kasemo, F and Hook, F: A combined
reflectometry and quartz crystal microbalance with dissipation monitoring setup
30
Karin Markides 511124-1021
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Appendix C
for the investigation of biofunctionalized surfaces. Rev of Scientific Instruments
2007, in writing
62.
Vallkil, J., Larsson, C., Voros, J., Borrebaeck, C.A.K., Hook, F. and Wingren,
C. Design of self-addressable antibody microarrays; Generic DNA tagging of
non-purified probes. J. Prot. Res. 2007, in writing
2. Review articles, book chapters etc
1.
Höök, F. Development of a novel QCM technique for protein adsorption studies,
PhD-thesis (1997)
2.
Höök, F, Fant, C & Larsson, C Biofunctional surfaces studied by quartz crystal
microbalance with dissipation monitoring, Encyclopedia of Colloid and Surface
Science, (2002) p 774-791, invited
3.
Höök, F. & Rudh, M. The QCM technique for Biomacromoelcular recognition,
Bio Tech International, March issue, 2005, invited
4.
Höök, F. and Kasemo, B. The QCM technique for Biomacromoelcular
recognition: technical and theoretical aspects, The Springer Series on Chemical
Sensors and Biosensors, 2006, Ed. Otto S. Wolfbeis
3. Patents, demos etc
1.
Rodahl M, Hook F, Krozer A and Kasemo B. (1995): Piezo-electric crystal
microbalance device. Patent #: US6006589
2.
Rodahl M; Krozer A; Kasemo B; Hook F; Fredriksson C; Steel D Method and
apparatus for measuring properties and processes of cells at surfaces. (1997)
Patent #: AU6431898
3.
Hook, F.: Sensor for Detecting Biological Matter. (1997) Patent #:
CA19982311398
4.
Pfeiffer I, Hook F, Svedhem S, Larsson C, Carlsson R: Immobilization of DNALabelled Lipid Vesicles on DNA arrays; (2003) Patent #: AU2003205600
5.
Granéli A., Reimhult E, Svedhem S, Pfeiffer I, and Hook F: Surface
immobilised multilayer structure of vesicles, (2003) Patent #: AU2004227314
6.
Pfeiffer, I. and Hook, F.: Oligonucleotides Related to Lipid Membrane
Attachments, (2004) Patent # CA2558256
7.
Höök, F and Rodahl, M: A mirrored Quartz Crystal Microbalance (2007), USprovisional, submitted
4. Popular Science
1.
Höök, F. and Kasemo, B. Funktionella ytor i biologiska system; grundforskning
och tillämpningar. Kosmos, Swedish Science Press, Ed. J.E. Thun, (2002), 79,
67-87
31
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Appendix C
Publications Mikael Käll
10 most important publications
H. Xu, E.J. Bjerneld, M. Käll, and L. Börjesson, ”Spectroscopy of Single Hemoglobin
Molecules by Surface Enhanced Raman Scattering”, Physical Review Letters 83, 43574360 (1999). 321 citations
H. Xu, J. Aizpurua, M. Käll, and P. Apell, “Electromagnetic contributions to singlemolecule sensitivity in surface-enhanced Raman scattering”, Physical Review E 62,
4318-4324 (2000). 207 citations
J. Aizpurua, P. Hanarp, D.S. Sutherland, M. Käll, G.W. Bryant, and F.J. García de
Abajo, “Optical properties of gold nanorings”, Physical Review Letters 90, # 057401
(2003). 89 citations
C.L. Haynes, A.D. McFarland, L. Zhao, R.P. Van Duyne, G.C. Schatz, L. Gunnarsson,
J. Prikulis, B. Kasemo, and M. Käll, “Nanoparticle optics: The importance of radiative
dipole coupling in two-dimensional nanoparticle arrays”, Journal of Physical
Chemistry B 107, 7337-7342 (2003). 87 citations
H. Xu and M. Käll, “Surface-plasmon enhanced optical forces in silver
nanoaggregates”, Physical Review Letters 89, # 246802 (2002). 66 citations
E.J. Bjerneld, Z. Földes-Papp, M. Käll, and R. Rigler, “Single-molecule surfaceenhanced Raman and fluorescence correlation spectroscopy of horse-radish
peroxidase”, J. Phys. Chem. B 106, 1213-1218 (2002). 51 citations
L. Gunnarsson, E.J. Bjerneld, H. Xu, S.Petronis, B. Kasemo and M. Käll,
“Interparticle-coupling effects in Nanofabricated Substrates for Surface Enhanced
Raman Scattering”, Appl. Phys. Lett. 78, 802-804 (2001). 45 citations.
L. Gunnarsson, T. Rindzevicius, J. Prikulis, B. Kasemo, M. Käll, S. Zou, and G.C.
Schatz, “Confined plasmons in nanofabricated single silver particle pairs –
Experimental observations of strong interparticle interactions”, Journal of Physical
Chemistry B 109, 1079-1087 (2005). 32 citations
P. Hanarp, M. Käll, and D. Sutherland; Optical properties of short-range ordered arrays
of nanometer gold disks prepared by colloidal lithography”, Journal of Physical
Chemistry B 107, 5768-5772 (2003). 30 citations
H. Xu and M. Käll, “Polarization dependent surface-enhanced Raman spectroscopy of
isolated silver nanoaggregates”, ChemPhysChem 4, 1001-1005 (2003). 28 citations
32
Karin Markides 511124-1021
Supra Centre Chalmers
Appendix C
2003-2007
Book Chapters
1.
H.X. Xu and M. Käll
Estimating SERS properties of silver-particle aggregates through generalized
Mie theory
Topics of Applied Physics 103, 87-104 (2006), edited by K. Kneipp, H. Kneipp
and M. Moskovits, Springer Verlag Berlin, Heidelberg 2006.
Journal Papers
105. J.B. González-Días, A. García-Martín, J.M. García-Martín, A. Cebollada, G.
Armelles, Y. Alaverdyan, B. Sépulveda, and M. Käll
Plasmonic Au/Co/Au nanosandwiches with enhanced magneto-optical-activity.
Small, accepted for publication (2007).
104. Z.P. Li, H.X. Xu, and M. Käll
Optical forces on interacting plasmonic nanoparticles in a focussed Gaussian
beam.
Physical Review E, accepted for publication (2007).
103. B. Sépulveda, J. Alegret, and M. Käll
Nanometric control of the distance between plasmonic nanoparticles using
optical forces.
Optics Express, in press (2007).
102. Y. Alaverdyan, B. Sépulveda, L. Eurenius, E. Olsson, and M. Käll
Optical nanoantennas based on nanoholes in thin metal films
Nature Physics, in press (2007).
101. T. Rindzevicius, Y. Alaverdyan, W.A. Murray, W.L. Barnes, and M. Käll
Long-range refractive index sensing using plasmonic nanostructures.
Journal of Physical Chemistry C 111, 11806-11810 (2007).
100. J. Andreasson, J. Holmlund, C.S. Knee, M. Käll, L. Börjesson, S. Naler, J.
Bäckström, M. Rübhausen, A.K. Azad and S.-G. Eriksson
Frank-Condon higher order lattice excitations in LaFe1-xCrxO3 (x=0, 0.1, 0.5,
0.1, 1.0) perovskites due to Fe-Cr charge transfer effects..
Physical Review B 75 #104302 (2007).
99.
J. Alegret, M. Käll, and P. Johansson
Top-down extended meshing algorithm and its applications to Green's tensor
nano-optics calculations.
Physical Review E 75 #046702 (2007).
33
Karin Markides 511124-1021
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Appendix C
98.
E.M. Larsson, J. Alegret, M. Käll, and D.S. Sutherland
Sensing characteristics of NIR localized surface plasmon resonances in gold
nanorings for application as ultrasensitive biosensors.
Nano Letters 7, 1256-1263 (2007).
97.
A. Dmitriev, T. Pakizeh, M. Käll, and D.S. Sutherland
Gold-silica-gold nanosandwiches: tunable bimodal plasmonic resonators.
Small 3, 294-299 (2007).
96.
T. Rindzevicius, Y. Alaverdyan, B. Sepulveda, T. Pakizeh, M. Käll, R.
Hillenbrand, J. Aizpurua, and F.J. Garcia de Abajo
Nanohole plasmons in optically thin gold films
Journal of Physical Chemistry C 111, 1207-1212 (2007).
95.
F. Svedberg, Y. Alaverdyan, P. Johansson, and M. Käll
Raman spectroscopic studies of terthiophenes for molecular electronics
Journal of Physical Chemistry B 110: 25671-25677 (2006).
94.
F. Svedberg, Z.P. Li, H.X. Xu, and M. Käll.
Creating hot nanoparticle pairs for surface-enhanced Raman spectroscopy
through optical manipulation
Nano Letters 6, 2639-2641 (2006).
93.
J. Holmlund, J. Andreasson, CS Knee, J. Bäckström, M. Käll, M. Osada, T.
Noji, Y. Koike, M. Kakihana, and L. Börjesson
Resonant two-phonon Raman scattering as a probe of hole crystal formation in
Sr14-xCaxCu24O41
Physical Review B 74, # 134502 (2006).
92.
M. Osada, M. Kakihana, H. Yasuoka, M. Käll, and L. Börjesson
Photoinduced nanodots in Bi2Sr2CaCu2O8+x
Key Engineering Materials 320: 167-170 (2006)
91.
M. Osada, M. Kakihana, H. Yasuoka, M. Käll, and L. Börjesson
Photoinduced nanodots and pinning effects in Bi2Sr2CaCu2O8+x
Physica C 445-448, 443-446 (2006).
90.
T. Pakizeh, M.S. Abrishamian, N. Granpayeh, A. Dimitriev and M. Käll.
Magnetic-field enhancement in gold nanosandwiches
Optics Express 14, 8240-8246 (2006).
89.
F. Svedberg and M. Käll.
On the importance of optical forces in surface-enhanced Raman scattering.
Faraday Discussions 132, 35-44 (2006).
88.
Y. Alaverdyan, P. Johansson, and M. Käll.
Photo-induced transformations in 2,2’:5’,2’’-terthiophene thin films on silver..
Physical Chemistry Chemical Physics 8, 1445-1450 (2006).
87.
T. Ambjörnsson, G. Mukhopadhyay, S.P. Apell and M. Käll.
34
Karin Markides 511124-1021
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Appendix C
Resonant coupling between localized plasmons and anisotropic molecular
coatings in ellipsoidal metal nanoparticles.
Physical Review B 73, #085412 (2006).
86.
T. Rindzevicius, Y. Alaverdyan, A. Dahlin, F. Höök, D. Sutherland, and M.
Käll.
Plasmonic sensing charactersistics of single nanometric holes.
Nano Letters 5, 2335-2339 (2005).
85.
F. Le, N.Z. Lwin, J.M. Steele, M. Käll, N.J. Halas, and P. Nordlander.
Plasmons in the metallic nanoparticle-film system as a tunable impurity
problem.
Nano Letters 5, 2009-2013 (2005).
84.
P. Johansson, H.X. Xu, and M. Käll.
Surface-enhanced Raman scattering and fluorescence near metal surfaces.
Physical Review B 72, #035427 (2005).
83.
M. Osada, M. Käll, J. Bäckström, M. Kakihana, N.H. Andersen, and L.
Börjesson
In situ resonant Raman scattering and reversible photoinduced structural
change in YBa2Cu3O6+x
Physical Review B 71, #214503 (2005).
82.
C.S. Knee, J. Holmlund, J. Andreasson, M. Käll, S.G. Eriksson, and L.
Börjesson
Order-disorder-order phase transition in the pyrochlore superconductor
Cd2Re2O7
Physical Review B 71, #214518 (2005).
81.
M. Käll, H.X. Xu, and P. Johansson
Field-enhancement and molecular response in surface-enhanced Raman and
fluorescence spectroscopy
Journal of Raman Spectroscopy 36, 510-514 (2005).
80.
E.M. Hicks, S. Zou, G.C. Schatz, K.G. Spears, R.P. Van Duyne, L. Gunnarsson,
T. Rindzevicius, B. Kasemo, and M. Käll
Controlling plasmon line-shapes through diffractive coupling in linear arrays of
cylindrical nanoparticles fabricated by electron beam lithography.
Nano Letters 5, 1065-1070 (2005).
79.
A. Dahlin, M. Zäch, T. Rindzevicius, M. Käll, D.S. Sutherland, and F. Höök.
Localized surface plasmon resonance sensing of lipid-membrane-mediated
biorecognition events
Journal of the American Chemical Society 127, 5043-5048 (2005).
78.
K. Ramser, J. Enger, M. Goksör, D. Hanstorp, K. Logg, and M. Käll
A microfluidic system enabling Raman measurements of the oxygenation cycle
in single optically trapped red blood cells.
LAB ON A CHIP 5, 431-436 (2005).
35
Karin Markides 511124-1021
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Appendix C
77.
L. Gunnarsson, T. Rindzevicius, J. Prikulis, B. Kasemo, M. Käll, S. Zou, and
G.C. Schatz
Confined plasmons in nanofabricated single silver particle pairs –
Experimental observations of strong interparticle interactions
Journal of Physical Chemistry B 109, 1079-1087 (2005).
76.
H. Xu, X-H. Wang, M.P. Persson, H.Q. Xu, M. Käll, and P. Johansson
Unified treatment of fluorescence and Raman scattering processes near metal
surfaces
Physical Review Letters 93, #243002 (2004).
75.
J. Aizpurua, L. Blanco, P. Hanarp, D.S. Sutherland, M. Käll, G.W. Bryant, and
F.J. Garcia de Abajo
Light scattering in gold nanorings
Journal of Quantitative Spectroscopy & Radiative Transfer 89, 11-16 (2004).
74.
J. Prikulis, P. Hanarp, L. Olofsson, D. Sutherland, and M. Käll
Optical spectroscopy of nanometric holes in thin gold films
Nano Letters 4, 1003-1007 (2004).
73.
J. Prikulis, F. Svedberg, M. Käll, J. Enger, K. Ramser, M. Goksör, and D.
Hanstorp
Optical manipulation and characterization of individual resonant metal
nanoparticles in solution
Nano Letters 4, 115-118 (2004).
72.
E.J. Bjerneld, F. Svedberg, P. Johansson, and M. Käll
Direct observation of heterogeneous photochemistry on aggregated Ag
nanocrystals using Raman spectroscopy: the case of photo-induced degradation
of aromatic amino acids.
Journal of Physical Chemistry A 108, 4187-4193 (2004).
71.
K. Ramser, K. Logg, M. Goksör, M. Käll, and D. Hanstorp
Resonance Raman spectroscopy of optically trapped functional erythrocytes.
Journal of Biomedical Optics 9, 593-600 (2004).
70.
L. Olofsson, T. Rindzevicius, I. Pfeiffer, M. Käll, and F. Höök
A gold-nanoparticle sensor for highly specific DNA hybridization detection.
Langmuir 19, 10414-10419 (2003).
69.
M.v. Zimmermann, J.R. Schneider, T. Frello, N.H. Andersen, J. Madsen, M.
Käll, H.F. Poulsen, R. Liang, P. Dosanjh, and W.N. Hardy
Oxygen-ordering superstructures in underdoped YBa2Cu3O6+x studied by hard
x-ray diffraction
Physical Review B 68, #104515 (2003).
68.
H. Xu and M. Käll
36
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Appendix C
Polarization dependent surface-enhanced Raman spectroscopy of isolated silver
nanoaggregates.
ChemPhysChem 4, 1001-1005 (2003).
67.
C.L. Haynes, A.D. McFarland, L. Zhao, R.P. Van Duyne, G.C. Schatz, L.
Gunnarsson, J. Prikulis, B. Kasemo, and M. Käll
Nanoparticle optics: The importance of radiative dipole coupling in twodimensional nanoparticle arrays..
Journal of Physical Chemistry B 107, 7337-7342 (2003).
66.
P. Hanarp, M. Käll, and D. Sutherland
Optical properties of short-range ordered arrays of nanometer gold disks
prepared by colloidal lithography.
Journal of Physical Chemistry B 107, 5768-5772 (2003).
65.
E.J. Bjerneld, F. Svedberg, and M. Käll
Laser-induced growth of noble metal nanoparticles for surface-enhanced
Raman scattering.
Nano Letters 3, 593-596 (2003).
64.
K. Ramser, E.J. Bjerneld, C. Fant, and M. Käll
Importance of substrate and photo-induced effects in Raman spectroscopy of
single functional erythrocytes.
Journal of Biomedical Optics 8, 173-178 (2003).
63.
J. Prikulis, K.V.G.K. Murty, H. Olin and M. Käll
Large-area topography analysis and near-field Raman spectroscopy using bent
fibre probes.
Journal of Microscopy 210, 269-273 (2003).
62.
J. Aizpurua, P. Hanarp, D.S. Sutherland, M. Käll, G.W. Bryant, and F.J. García
de Abajo
Optical properties of gold nanorings.
Physical Review Letters 90, # 057401 (2003).
37
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Appendix C
Publications Lisbeth Olsson
2003-2007 + important (*).
Papers in international journals with review:
(J23) *Østergaard, S., L. Olsson, M. Johnston and J. Nielsen (2000)
Increasing galactose consumption by Saccharomyces cerevisiae through
metabolic engineering of the GAL gene regulatory network. Nature
Biotechnology, 18, 1283-1286.
(J34) Roca, C., and L. Olsson (2003)
Increasing ethanol productivity during xylose fermentation by cell
recycling of recombinant Saccharomyces cerevisiae. Applied Microbiology
and Biotechnology, 60, 560-563.
(J35) Klinke, H. B., L. Olsson, A. B. Thomsen and B. K. Ahring (2003)
Potential inhibitors from wet oxidation of wheat straw and their effect
on ethanol production in Saccharomyces cerevisiae: wet oxidation and
fermentation by yeast. Biotechnology and Bioengineering, 81, 738-747.
(J36) Thygesen, A., A. B. Thomsen, A. S. Schmidt, H. Jørgensen, B. K. Ahring
and L. Olsson (2003)
Production of cellulose and hemicellulose-degrading enzymes by
filamentous fungi cultivated on wet-oxidised wheat straw. Enzyme and
Microbial Technology, 32, 606-615.
(J37) Jørgensen, H., J. P. Kutter, and L. Olsson (2003)
Separation and quantification of cellulases and hemicellulases by
capillary electrophoresis.
Analytical Biochemistry, 317, 85-93.
(J38) Lei, F., L. Olsson and S. B. Jørgensen (2003)
Experimental investigations of multiple steady states in aerobic
continuous cultivations of Saccharomyces cerevisiae. Biotechnology and
Bioengineering, 82, 766-777.
(J39) Jørgensen, H., T. Eriksson, J. Börjesson, F. Tjerneld and L.Olsson (2003)
Purification and characterization of five cellulases and one xylanase
from Penicillium brasilianum
IBT 20888. Enzyme and Microbial
Technology, 32, 851-861.
(J40) dos Santos, M. M., A. K.Gombert, B. Christensen, L. Olsson and J.
Nielsen (2003)
Identification of in vivo enzyme activities in the cometabolism of
glucose and acetate by Saccharomyces cerevisiae by using 13C-labeled
substrates. Eukaryotic Cell, 2, 599-608.
38
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Appendix C
(J41)* Roca, C., J. Nielsen and L.Olsson (2003)
Metabolic engineering of ammonium assimilation in xylose-fermenting
Saccharomyces cerevisiae improves ethanol production. Applied and
Environmental Microbiology, 69, 4732-4736.
(J42) Olsson, L., T. M. I. E. Christensen, K. P. Hansen, E. A. Palmqvist (2003)
Influence of the carbon source on production of cellulases,
hemicellulases and pectinases by Trichoderma reesei Rut C-30. Enzyme
and Microbial Technology, 33, 612-619.
(J43) dos Santos, M. M., G. Thygesen, P. Kötter, L. Olsson and J. Nielsen
(2003) Aerobic physiology of redox-engineered Saccharomyces cerevisiae
strains modified in the ammonium assimilation for increased NADPH
availability. FEMS Yeast Research, 4, 59-68.
(J44) Møller, K., R. B. Langekjær, J. Nielsen, J. Piškur and L.Olsson (2004)
Pyruvate decarboxylase from the petite-negative yeast Saccharomyces
kluyveri. Molecular Genetics and Genomics, 270, 558-568.
(J45) Roca, C., M. B. Haack and L. Olsson (2004)
Engineering of carbon catabolite repression in recombinant xylose
fermenting Saccharomyces cerevisiae. Applied Microbiology and
Biotechnology, 63, 578-583.
(J46) Krogh, K. B. R., A. Mørkeberg, H. Jørgensen, J. C. Frisvad and L.
Olsson (2004)
Screening genus Penicillium for producers of cellulytic and xylanolytic
enzymes. Applied Biochemistry and Biotechnology, 113-116, 389-401.
(J47) Jørgensen, H., A. Mørkeberg, K. B. R. Krogh and L. Olsson (2004)
Growth and enzyme production by three Pencillium species on
monosaccharides. Journal of Biotechnology, 109, 295-299.
(J48) Sonderegger, M., M. Jeppsson, C. Larsson, M.-F. Gorwa-Grauslund, E.
Boles, L. Olsson, I. Spencer-Martins, B. Hahn-Hägerdal and U. Sauer
(2004)
Fermentation performance of engineered and evolved xylosefermenting Saccharomyces cerevisiae strains. Biotechnology and
Bioengineering, 87, 90-98.
(J49) Møller, K., M. Z. Sharif, and L. Olsson (2004)
Production of fungal α-amylase by Saccharomyces kluyveri in glucoselimited cultivations. Journal of Biotechnology, 111, 311-318.
(J50) Haack, M. B., A. Eliasson, and L. Olsson (2004)
On-line cell mass monitoring of Saccharomyces cerevisiae cultivations by
multi-wavelength fluorescence. Journal of Biotechnology, 114, 199-208.
(J51)* dos Santos, M. M., V. Raghevendran, P. Kötter, L. Olsson and J.
Nielsen (2004) Manipulation of malic enzyme in Saccharomyces
cerevisiae for increasing NADPH production capacity aerobically in
different cellular compartments. Metabolic Engineering, 6, 352-363.
39
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Appendix C
(J52) Westergaard, S. L., C. Bro, L. Olsson and J. Nielsen (2004)
Elucidation of the role of Grr1p on glucose sensing by Saccharomyces
cerevisiae through genome-wide transcription analysis. FEMS Yeast
Research, 5, 193-204.
(J53) Lei, F., L. Olsson, and S. B. Jørgensen (2004)
Dynamic effects related to steady-state multiplicity in continuous
Saccharomyces cerevisiae strains. Biotechnology and Bioengineering, 88, 838848.
(J54)* Jørgensen, H., A. Mørkeberg, K. B. R. Krogh and L. Olsson (2005)
Production of cellulases and hemicellulases by three Pencillium species:
effect of substrate and evaluation of cellulose adsorption by capillary
electrophoresis. Enzyme and Microbial Technology, 36, 42-48.
(J55) Panagiotou, G., P. Christakopoulos, S.G. Villas-Boas and L. Olsson
(2005)
Fermentation performance and intracellular metabolite profiling of
Fusarium oxysporum cultivated on a glucose-xylose mixture. Enzyme and
Microbial Technology, 36, 100-106.
(J56) Panagiotou, G., Villas-Bôas, S. G., Christakopoulos P., Nielsen, J., and
L. Olsson (2005)
Intracellular metabolite profiling of Fusarium oxysporum converting
glucose to ethanol. Journal of Biotechnology, 115, 425-434.
(J57) Panagiotou, G., P. Christakopoulos and L.Olsson (2005)
Simultaneous saccharification and fermentation of cellulose by
Fusarium oxysporum F3- growth characteristics and metabolite
profiling. Enzyme and Microbial Technology, 36, 693-699.
(J58)* Devantier, R., S. Pedersen, and L. Olsson (2005)
The genome-wide transcriptional response of Saccharomuces cerevisiae
during very high gravity ethanol fermentations is highly affected by
the stationary phase. Industrial Biotechnology, 1, 51-63.
(J59) Devantier, R., B. Scheithauer, S. G. Villas-Bôas, S. Pedersen and L. Olsson
(2005).
Metabolite profiling for analysis of yeast stress response during very high
gravity ethanol fermentations.
Biotechnology and Bioengineering, 90, 703-714.
(J60) Zaldivar, J., C. Roca, C. Le Foll, B. Hahn-Hägerdal and L. Olsson (2005)
Ethanolic fermentation of acid pretreated starch industry effluents by
recombinant Saccharomyces cerevisiae strains. Bioresource Technology, 96,
1670-1676.
(J61)* Panagiotou, G., P. Christakopoulos and L.Olsson (2005)
The influence of different cultivation conditions on the metabolome of
Fusarium oxysporum, Journal of Biotechnology, 118, 304-315.
40
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Appendix C
(J62) Devantier, R., S. Pedersen and L. Olsson (2005)
Characterization of very high gravity ethanol fermentation of corn
mash. Effect of glucoamylase dosage, pre-saccharification and yeast
strain. Applied Microbiology and Biotechnology. 68, 622-629.
(J63) Bro, C., S. Knudsen, B. Regenberg, L. Olsson and J. Nielsen (2005)
Improvement of galactose uptake in Saccharomyces cerevisiae through
overexpression of phosphoglucomutase: exampe of transcript analysis
as a tool in inverse metabolic engineering. Applied and Environmental
Microbiology, 71, 6465-6472.
(J64) Raghevendran, V., J. Nielsen, and L. Olsson (2005)
Teaching microbial physiology using glucose repression phenomenon
in Baker’s yeast as an example. Biochemistry and Molecular Biology
Education, 33, 404-410.
(J65) Grotkjær, T., P. Christakopoulos, J. Nielsen, and L. Olsson (2005)
Comparative metabolic network analysis of two xylose fermenting
recombinant Saccharomyces cerevisiae strains. Metabolic Engineering, 7,
437-444
(J66) Jørgensen, H. and L.Olsson (2006)
Production of cellulases by Penicillium brasilianum IBT 20888 – Effect of
substrate on hydrolytic performance, Enzyme and Microbial Technology,
38, 381-390.
(J67) Eliasson Lantz, A., P. Jørgensen, E. Poulsen, C. Lindemann and L.
Olsson (2006)
Determination of cell mass and polymyxin using multi-wavelength
fluorescence. Journal of Biotechnology, 121, 544-554.
(J68) Haack, M. B., L. Olsson, K. Hansen and A. Eliasson Lantz (2006)
Change in hyphal morphology of Aspergillus oryzae during fed-batch
cultivation. Applied Microbiology and Biotechnology, 70, 482-487.
(J69) Olsson L., H.R. Soerensen, B.P. Dam, H. Christensen, K.M. Krogh and
A.S. Meyer (2006)
Separate and simultaneous enzymatic hydrolysis and fermentation of
wheat hemicellulose
with recombinant xylose utilizing Saccharomyces cerevisiae. Applied
Biochemistry and Biotechnology, 129-132, 117-129.
(J70) Raghevendran, V., K.R. Patil, L. Olsson and J. Nielsen (2006)
Hap4 is not essential for activation of respiration at low specific growth
rates in Saccharomyces cerevisiae. Journal of Biological Chemistry, 281,
12308-12314.
(J71)* Panagiotou, G., Christakopoulos, P., Grotkjær, T., and L. Olsson (2006)
Engineering of the redox imbalance of Fusarium oxysporum enables
anaerobic growth on xylose.
Metabolic Engineering, 8, 474 -482.
41
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Appendix C
(J72) Panagiotou, G., P. Granouillet and L. Olsson (2006)
Production and partial characterization of arabinoxylan-degrading
enzymes by Penicillium brasilianum under solid-state fermentation.
Applied Microbiology and Biotechnology, 72, 1117-1124.
(J73) Westergaard, S. L., A. P. Oliviera, C. Bro, L. Olsson and J. Nielsen
(2007)
A systems biology approach to study glucose repression in the yeast
Saccharomyces cerevisiae. Biotechnology and Bioengineering. 96, 134-145.
(J74) Panagiotou, G. and L. Olsson (2007)
Effect of compounds released during pretreatment of wheat straw on
microbial growth and enzymatic hydrolysis rates. Biotechnology and
Bioengineering. 96, 250-258
(J75) * Vemuri, G.N., Eiteman, M.A., McEwen, J.E., Olsson L. and J. Nielsen
(2007) Increasing NADH oxidation reduces overflow metabolism in
Saccharomyces cerevisiae
PNAS, 104, 2402-2407.
(J76)* Haack, M. B., A. Eliasson Lantz, P. P. Mortensen and L. Olsson (2007)
Chemometric analysis of in-line multi-wavelength fluorescence
measurements obtained during cultivations with a lipase producing
Aspergillus oryzae. Biotechnology and Bioengineering. 96, 904-913.
(J77) Meijer S, Panagiotou G, Olsson L, Nielsen J. (2007)
Physiological characterization of xylose metabolism in Aspergillus niger
under oxygen limited conditions. Biotechnology and Bioengineering. 98,
462-475
(J78) Panagiotou G., Olavarria, R., and L. Olsson (2007)
Penicillium brasilianum as an enzyme factory; the essential role of
feruloyl esterases for the hydrolysis of the plant cell wall. Journal of
Biotechnology. 130, 219-228
(J79) Panagiotou G., I. Kouskoumvekaki, S.O. Jónsdóttir and L. Olsson
(2007)
Monitoring novel metabolic pathways using metabolomics and
machine learning; induction of the phosphoketolase pathway in
Aspergillus nidulans cultivations. Metabolomics. In press. Published online.
(J80) Usaite, R., J. Nielsen and L. Olsson (2007)
Physiological characterization of glucose repression in the strains with
SNF1 and SNF4 genes deleted. Journal of Biotechnology, In press.
Published on-line 17. September 2007
42
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Appendix C
Reviews in peer reviewed journals:
(R1)
Olsson, L. and B. Hahn-Hägerdal (1996)
Fermentation of lignocellulosic hydrolysates for ethanol production.
Enzyme and Microbial Technology, 18, 312-331.
(R2)
Olsson, L. and J. Nielsen (1997)
On-line and in situ monitoring of biomass in submerged cultivations.
Trends in Biotechnology, 15, 517-522.
(R3)
Klein, C. J. L., L. Olsson and J. Nielsen (1998)
Glucose control in Saccharomyces cerevisiae: the role of MIG1 in metabolic
functions. Microbiology, 144, 13-24.
Olsson, L., U. Schulze and J. Nielsen (1998)
On-line bioprocess monitoring - an academic discipline or an industrial
tool? Trends in Analytical Chemistry, 17, 88-95.
Østergaard, S., L. Olsson and J. Nielsen (2000)
Metabolic engineering of Saccharomyces cerevisiae. Microbiology and
Molecular Biology Reviews, 64, 34-50.
(R4)
(R5)
(R6)
Olsson, L. and J. Nielsen (2000)
The role of metabolic engineering in the improvement of Saccharomyces
cerevisiae: utilization of industrial media. Enzyme and Microbial
Technology, 26, 785-792..
(R7)
Zaldivar, J., J. Nielsen and L. Olsson (2001)
Fuel ethanol production from lignocellulose: a challenge for metabolic
engineering and process integration. Applied Microbiology and
Biotechnology, 56, 17-34.
(R8)
Nielsen, J., and L. Olsson (2002)
An expanded role for microbial physiology in metabolic engineering
and functional genomics: moving towards systems biology. FEMS
Yeast Research, 2, 175-181.
(R9)* Otero, J. M., Panagiotou, G. And L. Olsson (2007)
Fueling Industrial Biotechnology Growth with Bioethanol
Advances in Biotechnology and Biochemical Engineering. 108, 1-40.
Book contributions:
(B1)
Hahn-Hägerdal B., J. Hallborn, H. Jeppsson, L. Olsson, K. Skoog, and M.
Walfridsson (1993)
Chapter 10 Pentose Fermentation to Alcohol, In: Bioconversion of Forest and
Agricultural Plant Residues, J.N. Saddler, Ed. CAB International,
Wallingford. p: 231-290.
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Appendix C
Olsson L., H. Jørgensen, K. Krogh and C. Roca (2005)
Chapter 42 Bioethanol production from lignocellulosic material. In:
Polysaccharides: structural diversity and functional versatility, S. Dumitriu,
Ed. Second Edition, revised & expanded. Marcel Dekker, Inc, New York.
p: 957-993.
Conference proceedings:
(C1)
(C2)
(C3)
(C4)
(C5)
(C6)
Østergaard, S., L. Olsson and J.Nielsen (1998)
Metabolic control analysis of the Leloir pathway in Saccharomyces
cerevisiae
Proceedings of BioThermoKinetics In the Post Genomic Era. 8th international
meeting on Biothermokinetics. July 2-5, Fiskebäckskil, Sweden, 22-26.
Klinke, H.B., A.B. Thomsen, A. S. Schmidt, L. Olsson and B. K. Ahring
(2000)
Wet oxidation of wheat straw: Potential fermentation inhibitors for
ethanol production.
Proceedings of 1st World Conference and Exhibition on Biomass for Energy and
Industry and 11th European Conference on Biomass for Energy and Industry and
the Biomass Conference of the Americas, Sevilla, Spain, 5-9 June, Spain.
Gombart, A. K., M. Moreira dos Santos, B. Christensen, L.Olsson and J. Nielsen
(2000)
Does the Mig1 protein affect the flux distribution in the central
metabolism of Saccharomyces cerevisiae?
Proceedings of 4th International Congress on Biochemical Engineering, 17-18
February, 2000, Stuttgart, Germany.
Ostergaard, S., L. Olsson and J. Nielsen (2001)
Metabolic Pathway Analysis of Saccharomyces cerevisiae. pp. 75-85. In:
Applied Microbiology. Focus on Biotechnology Vol. 2. Eds. M. Hofman, P.
Thonart, Kluwer Academic Publishers, Dordrecht.
Lei, F., L. Olsson, and S. B. Jørgensen (2001)
Investigation of multiple steady-states in continuous cultivation of
Saccharomyces cerevisiae.
Proceedings of 8th International Conference on Computer Applications in
Biotechnology. Quebec, Canada, June 24-27
Olsson, L. (2004)
Conference report. Physiology of yeasts and filamentous fungi II (PYFF-2),
Anglet, France, March 24-28,2004, FEMS Yeast Research 4 (2004) 891-892.
44
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Appendix C
Other publications:
(OP1) Olsson, L. (2000)
Bioteknologi fra første dag på DTU
Dansk Kemi, 81 (12), 14-17.
(OP2) Pedersen, J. G., L. Olsson and A. Eliasson (2001)
Kombinationssensorer er lovende til bioteknologisk produktion
Dansk Kemi, 82, 4-6 (tillæg til nr 5).
(OP3) Jørgensen, H., and L. Olsson (2002)
Produktion af lignocellulosenedbrydende enzymer i skimmelsvampe
Dansk Kemi, 83 (11), 20-24.
(OP4) Olsson, L. and T. S. Petersen (2002)
Synspunkt: Bioteknologer skal blande sig i den etiske debat
Ingeniøren, Nr 36, 16-17.
(OP5) Møller, K., and L.Olsson (2003)
Heterolog proteinproduktion med gæren Saccharomyces kluyveri
Dansk kemi, 84 (5), 35-37.
(OP6) Devantier, R., Pedersen, S. and L. Olsson (2005)
Fremstilling af bioethanol – nutidens teknologi og fremtidens
udfodringer
Dansk kemi, 86 (2), 26-29
(OP7) Otero, J.M., Olsson L. and J.Nielsen (2007)
Industrial Biotech meets Industrial Systems Biology. Petrochemical
industry finding success by turning to biotechnology.
Genetic Engineering News, 27 (1), 28-31
(OP8) Olsson, L., J. M. Otero, K. Patil and J. Nielsen (2007)
Fra petrokemisk til biobaseret ravsyreproduktion
Dansk kemi, 88 (3), 24-26
(OP9) Stougaard, P., Frisvad, J. C. and L.Olsson (2007)
Cool Biotechnology
Dansk kemi, 88 (5), 22-25
45
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Appendix C
Publications Owe Orwar
LIST OF THE 10 BEST PUBLICATIONS
81. Tatsiana Lobovkina, Paul Dommersnes, Jean-François Joanny, Johan
Hurtig, and Owe Orwar Zipper Dynamics of Surfactant Nanotube
Junctions Physical Review Letters, 97, 188105, (2006)
75. Michal Tokarz, Björn Åkerman, Jessica Olofsson, Jean-Francois Joanny,
Paul Dommersnes and Owe Orwar. Single-file electrophoretic transport
and counting of individual DNA molecules in surfactant nanotubes
PNAS, 102, 9127-9132, (2005)
72. J. Olofsson, H. Bridle, J. Sinclair, E. Sahlin, D. Granfeldt, O. Orwar.
A chemical waveform synthesizer PNAS, 102, 8097-8102 (2005)
64. Tatsiana Lobovkina, Paul Dommersnes, Jean-Francois Joanny, Patricia
Bassereau, Mattias Karlsson, Owe Orwar Mechanical Tweezer Action by
Self-Tightening Knots in Surfactant Nanotubes PNAS, 101, 7949-7953
(2004)
48. M. Karlsson, K. Sott, M. Davidson, P. Linderholm, A-S Cans, O. Orwar.
Formation of Geometrically Complex Lipid Nanotube-Vesicle Networks
of Higher Order Topologies. PNAS, 99, 11573-11578 (2002)
45. A. Karlsson, M. Karlsson, R. Karlsson, A-S. Cans, Anette Strömberg,
Frida Ryttsén, O. Orwar. Networks of nanotubes and containers Nature,
409, 150-152 (2001)
32. D. T. Chiu, C. Wilson, F.Ryttsén, A. Strömberg, A. Karlsson, S.
Nordholm, A. Hsiao, A.Gaggar, R. Garzia-López, A. Moscho, O. Orwar,
R. N. Zare, Chemical transformations in ultrasmall biomimetic
containers, Science, 283, 1892 (1999)
28. J. A. Lundqvist, F. Sahlin, A. I. M. Åberg, A. Strömberg, P. Eriksson, O.
Orwar, Altering the biochemical state of individual cultured cells and
organelles using ultramicroelectrodes, PNAS, 95, 10356 (1998)
27. D. T. Chiu, S. J. Lillard, R. H. Scheller, R. N. Zare, S. E. Rodriguez
Cruz, E. R. Williams, O.Orwar, M. Sandberg, J. A. Lundqvist, Probing
single secretory vesicles with capillary electrophoresis Science, 279, 1190
(1998)
18. O. Orwar, K. Jardemark, I. Jacobson, A. Moscho, H.A. Fishman, R. H.
Scheller, R. N. Zare, Patch clamp detection of neurotransmitters in
capillary electrophoresis Science, 272, 1779 (1996)
46
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Appendix C
2003-2007
98. Ilja Czolkos, Yavuz Erkan, Paul Dommersnes, Aldo Jesorka, and Owe
Orwar. Controlled Formation and Mixing of Two-Dimensional Fluids.
Nano Letters; ASAP, Web Release Date: 06-Jun-2007, DOI:
10.1021/nl070726u, 2007.
97. Tokarz, M.; Hakonen, B.; Dommersnes, P.; Orwar, O. and Akerman, B.
Electrophoretic Transport of Latex Particles in Lipid Nanotubes.
Langmuir; ASAP, Web Release Date: June 5, (2007), DOI:
10.1021/la700336u, 2007.
96. Olofsson, J., Levin, M., Stromberg, A., Weber, S. G., Ryttsen, F., and
Orwar, O. Scanning Electroporation of Selected Areas of Adherent Cell
Cultures.
Analytical Chemistry; Advance article, May 19, 2007.
95. Lizana L, Konkoli Z, and Orwar O. Tunable Filtering of Chemical Signals
in a Simple Nanoscale Reaction-Diffusion Network.
J. Phys. Chem B; Advance article, May 12, 2007.
94. Agarwal, A., Zudans, I., Weber, E. A., Olofsson, J., Orwar, O., and Weber,
S. G. Effect of Cell Size and Shape on Single-Cell Electroporation.
Analytical Chemistry; 79(10),3589 -3596, E-pub Apr 20, 2007.
93. Direct Immobilization of Cholesteryl-TEG-Modified Oligonucleotides
onto Yavuz Erkan, Ilja Czolkos, Aldo Jesorka, L. Marcus Wilhelmsson,
and Owe Orwar. Hydrophobic SU-8 Surfaces.
Langmuir; 23(10),5259-63, (doi:10.1021/la7005502), 2007.
92. Lambie, B. A., Brennan, C., Olofsson, J., Orwar, O., and Weber, S. G.
Experimentally Determining the iR Drop in Solution at Carbon Fiber
Microelectrodes with Current Interruption and Application to Single-Cell
Electroporation.
Analytical Chemistry; 79(10),3771-8, 2007.
91. Zudans, I., Agarwal, A., Orwar O., and Weber, S. G. Numerical
calculations of single-cell electroporation with an electrolyte-filled
capillary.
Biophys J.; 92(10),3696-705, Epub: Mar 9, 2007.
90. Martin Markström, Anders Gunnarsson, Owe Orwar and Aldo Jesorka.
Dynamic microcompartmentalization of giant unilamellar vesicles by solgel transition and temperature induced shrinking/swelling of poly(Nisopropyl acrylamide).
Soft Matter; 3,587-595 (doi:10.1039/b610351k), 2007.
47
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Appendix C
89. Aparna Agarwal, Imants Zudans, Owe Orwar, and Stephen G. Weber.
Simultaneous Maximization of Cell Permeabilization and Viability in
Single-Cell Electroporation Using an Electrolyte-Filled Capillary.
Analytical Chemistry; 79(1), 161-167(doi:10.1021/ac061270o), 2007.
89. Daniel Granfeldt, Jon Sinclair, Maria Millingen, Cecilia Farre, Per
Lincoln, and Owe Orwar. Controlling Desensitized States in LigandReceptor Interaction Studies with Cyclic Scanning Patch-Clamp Protocols.
Analytical Chemistry (Accelerated Article); 78(23), 7947-7953
(doi:10.1021/ac060812z), 2006.
88. Tatsiana Lobovkina, Paul Dommersnes, Jean-François Joanny, Johan
Hurtig, and Owe Orwar. Zipper Dynamics of Surfactant Nanotube
Junctions.
Physical Review Letters; 97, 188105,
(doi:10.1103/PhysRevLett.97.188105), 2006
87. Brigitte Bauer, Max Davidson, and Owe Orwar. Direct Reconstitution of
Plasma Membrane Lipids and Proteins in Nanotube-Vesicle Networks.
Langmuir; 22(22),9329-9332 (doi:10.1021/la060828k), (2006)
86. Roger Karlsson, Anders Karlsson, Andrew Ewing, Paul Dommersnes,
Jean-Francois Joanny, Aldo Jesorka and Owe Orwar. Chemical Analysis
in Nanoscale Surfactant Networks.
Analytical Chemistry; 78(17), 5960 - 5968, (2006), (Review)
85. Johan Hurtig, Bodil Gustafsson, Michal Tokarz, and Owe Orwar.
Electrophoretic Transport in Surfactant Nanotube Networks Wired on
Microfabricated Substrates.
Analytical Chemistry; 78(15), 5281-5288, (2006), (Accelerated Article)
84. Bradley A. Lambie, Owe Orwar, and Stephen G. Weber. Controlling the
Electrochemically Active Area of Carbon Fiber Microelectrodes by the
Electrodeposition and Selective Removal of an Insulating Photoresist.
Analytical Chemistry; 78(14), 5165 - 5171, (2006)
83. Jon Sinclair, Daniel Granfeldt, Johan Pihl, Maria Millingen, Per Lincoln,
Cecilia Farre Lena Peterson and Owe Orwar. A Biohybrid Dynamic
Random Access Memory J. Am. Chem. Soc. 128(15),5109-5113 (2006)
82. Brigitte Bauer, Max Davidson, and Owe Orwar. Direct Reconstitution of
Plasma Membrane Lipids and Proteins in Nanotube-Vesicle Networks
Langmuir; 22(22),9329-9332 (2006)
81. Tatsiana Lobovkina, Paul Dommersnes, Jean-François Joanny, Johan
Hurtig, and Owe Orwar Zipper Dynamics of Surfactant Nanotube
Junctions Physical Review Letters; 97, 188105, (2006)
80. Roger Karlsson, Anders Karlsson, Andrew Ewing, Paul Dommersnes,
48
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Supra Centre Chalmers
Appendix C
Jean-Francois Joanny, Aldo Jesorka and Owe Orwar Chemical Analysis
in Nanoscale Surfactant Networks Analytical Chemistry; 78(17), 5960 5968, (2006), (Review)
80. Daniel Granfeldt, Jon Sinclair, Maria Millingen, Cecilia Farre, Per
Lincoln, and Owe Orwar Controlling Desensitized States in LigandReceptor Interaction Studies with Cyclic Scanning Patch-Clamp Protocols
Analytical Chemistry; 78(23), 7947-7953 (2006), (Accelerated Article)
79. Johan Hurtig, Bodil Gustafsson, Michal Tokarz, and Owe Orwar
Electrophoretic Transport in Surfactant Nanotube Networks Wired on
Microfabricated Substrates Analytical Chemistry; 78(15), 5281-5288,
(2006), (Accelerated Article)
78. Bradley A. Lambie, Owe Orwar, and Stephen G. Weber
Controlling the Electrochemically Active Area of Carbon Fiber
Microelectrodes by the Electrodeposition and Selective Removal of an
Insulating Photoresist Analytical Chemistry; 78(14), 5165 - 5171 (2006)
77. Kristin Sott, Tatsiana Lobovkina, Ludvig Lizana, Michal Tokarz, Brigitte
Bauer, Zoran Konkoli and Owe Orwar. Controlling Enzymatic Reactions
by Geometry in a Biomimetic Nanoscale Network. Nano Letters 6(2),
209-214, (2006)
76. Johan Pihl, Jon Sinclair, Mattias Karlsson, and Owe Orwar
Microfluidics for cell-based assays Materials Today; 8(12), 46 - 51,
(2005)
75. Michal Tokarz, Björn Åkerman, Jessica Olofsson, Jean-Francois Joanny,
Paul Dommersnes and Owe Orwar. Single-file electrophoretic transport
and counting of individual DNA molecules in surfactant nanotubes
PNAS; 102(26), 9127-9132, (2005)
74. Aldo Jesorka, Martin Markström, Mattias Karlsson, and Owe
Orwar. Controlled Hydrogel Formation in the Internal Compartment of
Giant Unilamellar Vesicles J. Physical Chemistry B; 109(31), 14759 14763 (2005)
73. J. Olofsson, F. Ryttsén, A. Strömberg, M. Levin, S.G. Weber, O. Orwar.
Generation of focused electric field patterns close to dielectric surfaces.
AnalChem; 77(14), 4667 - 4672, (2005)
72. J. Olofsson, H. Bridle, J. Sinclair, E. Sahlin, D. Granfeldt, O. Orwar.
A chemical waveform synthesizer. PNAS; 102(23), 8097-8102 (2005)
71. Johan Pihl, Jon Sinclair, Eskil Sahlin, Mattias Karlsson, Fredrik Petterson,
Jessica Olofsson, and Owe Orwar Microfluidic Gradient-Generating
Device for Pharmacological Profiling Analytical Chemistry; 77(13),
3897-3903 (2005)
49
Karin Markides 511124-1021
Supra Centre Chalmers
Appendix C
70. P. G. Dommersnes, O. Orwar, F. Brochard-Wyart and J. F. Joanny.
Marangoni transport in lipid nanotubes Europhysics Letters. 70(2), 271277, (2005)
69. Aldo Jesorka, Martin Markström, and Owe Orwar. Controlling the Internal
Structure of Giant Unilamellar Vesicles by Means of Reversible
Temperature Dependent Sol-Gel Transition of Internalized Poly(Nisopropyl acrylamide) Langmuir; 21(4); 1230-1237, (2005)
68. Anders Karlsson, Kristin Sott, Martin Markström, Max Davidson, Zoran
Konkoli, and Owe Orwar. Controlled Initiation of Enzymatic Reactions
in Micrometer-Sized Biomimetic Compartments J. Phys. Chem. B;
109(4); 1609-1617 (2005)
67. Max Davidson, Paul Dommersnes, Martin Markström, Jean-Francois
Joanny, Mattias Karlsson, and Owe Orwar. Fluid Mixing in Growing
Microscale Vesicles Conjugated by Surfactant Nanotubes. J. Am. Chem.
Soc.; 127(4); 1251-1257, (2005)
66. Johan Hurtig, Mattias Karlsson, and Owe Orwar Topographic SU-8
Substrates for Immobilization of Three-Dimensional Nanotube-Vesicle
Networks Langmuir, (2004) 20, 5637-5641
65. M. Karlsson, M. Davidson, R. Karlsson, A. Karlsson, J. Bergenholtz, Z.
Konkoli, A. Jesorka, T. Lobovkina, J. Hurtig, M. Voinova, and O. Orwar
Biomimetic Nanoscale Reactors and Networks Ann. Rev. Phys. Chem.;
(2004) 55, 613-649
64. Tatsiana Lobovkina, Paul Dommersnes, Jean-Francois Joanny, Patricia
Bassereau, Mattias Karlsson, Owe Orwar
Mechanical Tweezer Action by Self-Tightening Knots in Surfactant
Nanotubes PNAS; (2004) 101, 7949-7953
63. J. Olofsson, J. Pihl, J. Sinclair, M. Karlsson, E. Sahlin, O. Orwar. A
Microfluidic Approach to the Problem of Creating Separate Solution
Environments Accessible From Macroscopic Volumes. Anal.Chem.,
(2004) 76; 4968-4976
62. Zoran Konkoli, Anders Karlsson and Owe Orwar
The Pair Approach Applied to Kinetics in Restricted Geometries:
Strengths and Weaknesses of the Method J. Phys. Chem. B.; (2003) 107,
14077-14086
61. Jon Sinclair, Jessica Olofsson, Johan Pihl, and Owe Orwar
Stabilization of High-Resistance Seals in Patch-Clamp Recordings by
Laminar Flow Analytical Chemistry; (2003) 75, 6718-6722
60. Roger Karlsson, Anders Karlsson, and Owe Orwar
Formation and Transport of Nanotube-Integrated Vesicles in a Lipid
Bilayer Network J. Phys. Chem. B; (2003) 107, 11201-11207
50
Karin Markides 511124-1021
Supra Centre Chalmers
Appendix C
59. Ann-Sofie Cans, Nathan Wittenberg, Daniel Eves, Roger Karlsson, Anders
Karlsson, Owe Orwar, and Andrew Ewing Amperometric Detection of
Exocytosis in an Artificial Synapse Analytical Chemistry; (2003) 75,
4168-4175
58. Roger Karlsson, Anders Karlsson, and Owe Orwar A Nanofluidic
Switching Device J. Am. Chem. Soc.; (2003) 125, 8442 - 8443,
57. Anders Lundqvist, Daniel T. Chiu, and Owe Orwar
Electrophoretic separation and confocal laser-induced fluorescence
detection at ultralow concentrations in constricted fused-silica capillaries
Electrophoresis; (2003) 24, 1737 -1744
56. Anders Karlsson, Mattias Karlsson, Roger Karlsson, Kristin Sott, Anders
Lundqvist, Michal Tokarz, and Owe Orwar Nanofluidic Networks Based
on Surfactant Membrane Technology Anal. Chem.; (2003) 75, 2529 -2537
55. Kristin Sott, Mattias Karlsson, Johan Pihl , Johan Hurtig, Tatsiana
Lobovkina, and Owe Orwar Micropipet Writing Technique for Production
of Two-Dimensional Lipid Bilayer Nanotube-Vesicle Networks on
Functionalized and Patterned Surfaces Langmuir; (2003) 19, 3904-3910
54. Jessica Olofsson, Kerstin Nolkrantz, Frida Ryttsén, Bradley A. Lambie,
Stephen G. Weber, and Owe Orwar Single-cell electroporation Current
Opinion in Biotechnology; (2003) 14, 29-34
53. Ann-Sofie Cans, Nathan Wittenberg, Roger Karlsson, Leslie Sombers,
Mattias Karlsson, Owe Orwar, and Andrew Ewing Artificial cells: Unique
insights into exocytosis using liposomes and lipid nanotubes PNAS; (2003)
100, 400-404
52. Nanotube-Vesicle Networks with Functionalized Membranes and Interiors
Max Davidson, Mattias Karlsson, Jon Sinclair, Kristin Sott, and Owe
Orwar J. Am. Chem. Soc.; (2003) 125, 374-378
51
Karin Markides 511124-1021
Supra Centre Chalmers
Appendix C
Publications Björn Åkerman
10 selected publications
5. Kubista, M., B. Åkerman, & B. Norden. 1987. Characterization of the Interaction
between DNA and 4',6-Diamidino-2-phenylindole by Optical Spectroscopy.
Biochemistry 26, 4545-4553.
11. Magnusdottir, S., B. Åkerman & M. Jonsson. 1994. DNA Electrophoresis in
Agarose Gels: Three Regimes of DNA migration Identified and Characterized by the
Electrophoretic Orientational Behaviour of the DNA. J. Phys. Chem. 98, 2624-2633.
12. Larsson A. & B. Åkerman. 1995. Period times and helix alignment during the
cyclic migration of DNA in electrophoresis gels studied with fluorescence
microscopy. Macromolecules. 28, 4441-4454.
15. Åkerman, B. & E. Tuite. 1996 Single and double-stranded cleavage of DNA by
YO, YOYO and TOTO. Nucl. Acids Res. 24, 1080-1090.
30. Boncheva, M., Scheibler, L., Lincoln, P., Vogel, H., Åkerman, B. 1999. Design of
oligonucleotides at interfaces. Langmuir. 15, 4317-4320.
42. Sandström. P., Boncheva, M., Åkerman, B. 2003. Non-specific and thiol-specific
binding of DNA to colloidal gold particles. Langmuir 19, 7537-7543
50. Tokarz, M., Åkerman, B., Olofsson, J., Joanny, J.-F., Dommersnes, P., Orwar, O.
2005. Single-file electrophoretic transport and counting of individual DNA molecules
in surfactant nanotube. Proc. Nat. Acad. Sci. 102, 9127 – 9132.
53. Sanandaji, N., Carlsson, N., Voinova, M., Åkerman, B. 2006. Comparison of
oligonucelotide migration in a bicontinuous cubic phase of monoolein and water and
in a fibrous agarose hydrogel. Electrophoresis, 27, 3007-3017
55. Cole, K. D., Gaigalas, A., Åkerman, B. 2006 Single molecule measurements of
trapped and migrating circular DNA during electrophoresis in agarose gels.
Electrophoresis, 27, 4396-4407
56. Eriksson, M., Härdelin, M., Larsson, A., Bergenholtz, J., Åkerman, B. 2007
Binding of intercalating and groove-binding cyanine dyes to bacteriophage T5. J.
Phys. Chem. B 111, 1139-1148
2003-2007
42. Sandström. P., Boncheva, M., Åkerman, B. 2003. Non-specific and thiol-specific
binding of DNA to colloidal gold particles. Langmuir 19, 7537-7543
43. Karlsson, J., Eriksson, M., Perzon, E., Åkerman, B., Lincoln, P., Westman, G.
2003 Groove-binding unsymmetrical cyanine dyes for staining of DNA:synthesis and
characterisation of the DNA binding. Nucl. Acids Res. 31, 6227-6234
44. Eriksson, M., Karlsson, J.,Westman, G., Åkerman, B. 2003 Groove-binding
unsymmetrical cyanine dyes for staining of DNA: dissociation rates in free solution
and electrophoresis gels. Nucl. Acids Res. 31, 6235-6242
52
Karin Markides 511124-1021
Supra Centre Chalmers
Appendix C
45. Cole, K., Åkerman, B. 2003 The effect of gel concentration on the electrophoretic
capture of circular DNA. Separation Sci. Tech. 38, 2121-2136
46. Svingen, R, Åkerman, B. 2004. Mechanism of electrophoretic migration of DNA
in the cubic phase of Pluronic F127 and water. J. Phys. Chem. B 108, 2735-2743
47. Sandström. P., Åkerman, B. 2004. Electrophoretic properties of DNA-modified
colloidal gold particles. Langmuir 20, 4182-4186
48. Eriksson, M., Mehmedovic, Westman, G., Åkerman, B. 2005 Time-resolved
electrophoretic analysis of mobility shifts for dissociating ligands. Electrophoresis 26,
524-532
49. Dias, R.S., Svingen, R., Lindman, B., Miguel, M.G. Åkerman, B. 2005.
Electrophoretic properties of complexes between DNA and cationicsurfactant CTAB. .
Electrophoresis 26, 2908–2917
50. Tokarz, M., Åkerman, B., Olofsson, J., Joanny, J.-F., Dommersnes, P., Orwar, O.
2005. Single-file electrophoretic transport and counting of individual DNA molecules
in surfactant nanotube. Proc. Nat. Acad. Sci. 102, 9127 – 9132.
51. Carlsson, N., Winge, A.S., Svingen, R, Engström, S. Åkerman, B. 2005 Diamond
cubic phase of monoolein and water as an amphiphilic matrix for electrophoresis of
oligonucleotide. J. Phys. Chem. 109, 18268 - 18636
52. Carlsson, N., Sanandaji, N., Voinova, M., Åkerman, B. 2006. A bicontinuous
cubic phase of monoolein and water as medium for electrophoresis of both
membrane-bound probes and DNA. Langmuir, 22, 4408-4414
53. Sanandaji, N., Carlsson, N., Voinova, M., Åkerman, B. 2006. Comparison of
oligonucelotide migration in a bicontinuous cubic phase of monoolein and water and
in a fibrous agarose hydrogel. Electrophoresis, 27, 3007-3017
54. Eriksson, M., Westerlund, F., Mehmedovic, M., Lincoln, P., Larsson, A.,
Westman, G., Åkerman, B. 2006 Comparing mono- and divalent groove-binding
cyanine dyes. DNA-binding, fluorescence properties and dissociation properties.
Biophysical Chemistry, 122, 195-205
55. Cole, K. D., Gaigalas, A., Åkerman, B. 2006 Single molecule measurements of
trapped and migrating circular DNA during electrophoresis in agarose gels.
Electrophoresis, 27, 4396-4407
56. Eriksson, M., Härdelin, M., Larsson, A., Bergenholtz, J., Åkerman, B. 2007
Binding of intercalating and groove-binding cyanine dyes to bacteriophage T5. J.
Phys. Chem. B 111, 1139-1148
57. Tokarz, M., Hakonen, B., Domersnes, P., Orwar, O., Åkerman, B. 2007.
Electrophoretic transport of latex particles in lipid nanotubes. Langmuir 23, 76527658.
58. Åkerman, B., Cole, K. D. 2007 Electrophoretic stretching of circular DNA
studied by fluorescence microscopy and polarized spectroscopy. Submitted
Macromolecules
59. Tokarz, M., Orwar, O., Åkerman, B. 2007. Electrophoersis of DNA molecules in
deformable surfactant nanotubs – comparison with theory. Submitted Langmuir.
53
Kod
2007-14950-54750-89
Name of applicant
Markides, Karin
Date of birth
511124-1021
Title of research programme
Appendix U
Requirements for implementation
VRAPS/VR-Direct bilaga 2004.Re
Vetenskapsrådet, SE-103 78 Stockholm, tel. +46 (0)8 546 44 000, [email protected]
Karin Markides 511124-1021
SUPRA Centre
Appendix U
Appendix U – Resources and organisation
Chalmers is well established as a leading university of technology in Europe. This is based on
successful research and education in science, engineering, architecture and management. A
common denominator is the strong scientific foundation and the broad interaction with the
corporate sector. It is within this academic setting the SUPRA centre is proposed around a
group of well recognised scientists and highly relevant infrastructures.
The process of identifying areas for a grant proposal under the Linnaeus programme has been
highly selective and the proposals submitted are chosen primarily to suit the long term
development of Chalmers but also to justify that a grant under the programme would be
beneficial to the scientific development in Sweden as a whole. In the internal process the
proposals have been challenged by both internal as well as external aspects in order to
improve the suggested research themes and to make them well recognised within Chalmers.
Thereby they have the potential to become part of multidimensional interactions with
different research groups, whether or not involved in the actual proposals. The entire faculty
is supportive of the choice of areas around which the proposals are submitted.
In this appendix a listing of the factual and expected economical resources are presented
divided into internal (Chalmers) and external support. This is followed by a budget for the
first 10 years and a sketch of the organisation of the SUPRA centre.
Financial plan for the SUPRA centre: the first ten years.
Support from Chalmers.
Data refer to actual figures for 2007. In addition, Chalmers will support each Linnaeus
recipient with 1000 kSEK per year.
Table 1. Support from Chalmers (in kSEK).
Year
1
2
3
4
5
6
7
8
9
10
12601 12979 13368 13769 14183 14608 15046 15498 15963
16441
Faculty
grant1
1000
1000
1000
1000
1000
1000
1000
1000
Extra support 1000 1000
from
Chalmers
13601 13979 14368 14769 15183 15608 16046 16498 16963
17441
Total
1
Total University grants, including grants from the Chalmers foundation, distributed to the 10
principal investigators based on the fiscal year 2007. We have assumed an adjustment
upwards of 3 % pa.
Support from external sources.
Given is the financial support from national and international external sources. Figures for
2007 are factual, and the figures for 2008 and 2009 are contracted funds. Naturally, new
contracts during 2008 and 2009 are expected but not counted in Table 1. In the 5th column we
give annual estimates for the Linnaeus grant period. All amounts in kSEK.
1
Karin Markides 511124-1021
SUPRA Centre
Table 2. External support to the 10 principle investigators (in kSEK).
2007
2008
2009
Year
Grant
National1
25 914
19 400
14 200
International2
6 250
3 200
1 200
3
Equipment
3 550
0
0
Total
35 714
22 600
15 400
1
Primarily VR, SSF, and KAW. 2 Primarily EU, NSF, and ESF. 3VR, KAW.
Appendix U
Estimates for the
Linnaeus Grant
period (per year)
30 000
8 000
4 000
42 000
Budget for the first 10 year period of the SUPRA Center:
The overreaching vision of the SUPRA Centre is to renew and strengthen the research
environment in the group and to initiate new cross-disciplinary research. With this in mind,
the proposed budget will be based on the following criteria:
- Staff renewal: The age structure of the group is such that a partial renewal of the staff will be
necessary during the grant period.
- Internationalization: It is our experience that a broad international network and frequent
international visits of post-docs and visiting scientists are important for the group in order to
guarantee that the quality of the research is maintained at the highest international level.
- Graduate education: The Center will strive to carry out graduate education of the highest
international standards.
- With these criteria in mind, the Center envisions to support a number of the assistant
professors presently hired in the group, with the goal of facilitating their promotion to
associate professors. A substantial support will be given for 4 years.
- For recruitments of post-docs, the Center plans to use a significant proportion of the grant
and almost half of the grant will be used for financing graduate students.
- In the recruitment process, SUPRA will pay special attention to gender aspects, such that
during the grant period, the gender balance will be significantly improved among the tenured
scientists and that a reasonable gender balance will be maintained among the graduate
students and visiting post doctoral students.
- Finally, SUPRA has set aside funds for communicating the results of the Center and for the
administration of the Center.
With this application, the Center seeks support from VR amounting to a total of 7.5 million
SEK/Year. Of the total amount received from Chalmers (as detailed above), we will use 3.75
million SEK/Year towards the running of the SUPRA Center. Thus in total, the annual budget
of the Center is 11.25 million SEK. Finally, we have chosen to distribute the funds spent
almost equally over the 10-year period. Depending on circumstances (e.g. finding suitable
candidates for the different positions), it may be that the funds spent pa. will vary somewhat,
but overall, the proportion of funds spent on each item in the budget will be as given below.
2
Karin Markides 511124-1021
SUPRA Centre
Appendix U
Table 3. Proposed budget (in kSEK).
Year
Grad. Students1
Post docs2
Promotion of AnnSofie Cans3
Promotion of Linda
Gunnarsson3
Promotion of Sofia
Svedhem3
Strategic
Recruitments4
Centre Administration
Dissemination of
results5
Infrastructure
(Overhead costs)
Total
1
10x500
=
5000
5x550
=
2750
2
10x500
=
5000
4x550
=
2200
500
3
10x500
=
5000
3x550
=
1650
500
4
10x500
=
5000
3x550
=
1650
500
5
10x500
=
5000
3x550
=
1650
500
6
10x500
=
5000
3x550
=
1650
500
500
500
500
500
500
500
500
7
10x500
=
5000
3x550
=
1650
8
10x500
=
5000
3x550
=
1650
9
10x500
=
5000
3x550
=
1650
10
10x500
=
5000
3x550
=
1650
725
725
725
725
250
250
250
300
250
300
250
300
250
300
250
300
250
300
250
300
250
300
250
300
3000
11250
3000
11250
3000
11700
3000
11700
3000
11700
3000
11200
3000
10925
3000
10925
3000
10925
3000
10925
1. Graduate students according to: 400 pa. salary and 100 pa. operating grant.
2. Post Doctoral students according to (on average): 450 pa. salary and 100 pa. operating grant.
3. Promotion of assistant professors (“Forskarassistenter”) to tenured positions.
4. Strategic recruitments to renew staff at the Center.
5. Publications, invited lectures, and support of graduate courses relevant for the PhD students enrolled at the Centre.
3
Karin Markides 511124-1021
SUPRA Centre
Appendix U
Scientists involved in the SUPRA Center.
Below we present the scientists that will be involved in the activities of the SUPRA Center.
Also given are their year of birth, year of obtaining PhD exams and their appointments, and
where appropriate, the termination of their present appointment.
Table 4. Participating scientists.
Name
Year of birth
PhD degree
Position
Bo Albinsson, PI
1963
1993
Professor
Mats Andersson, PI
1966
1995
Professor
Ann-Sofie Cans
1971
2003
Ass. Professor (-May 2009)
Annika Enejder
1969
1997
Assoc. Professor
Andrew Ewing#
1957
1983
Professor
Linda Gunnarsson, PI
1971
2004
Ass. Professor (-Jan 2010)
Krister Holmberg, PI
1946
1974
Professor
Fredrik Höök, PI
1966
1997
Professor
Nina Kann
1964
1993
Assoc. Professor
Zoran Konkoli
1966
1996
Assoc. Professor
Mikael Käll, PI
1963
1995
Professor
Sven Larsson
1941
1972
Professor
Per Lincoln
1958
1993
Assoc. Professor
Jerker Mårtensson
1965
1995
Assoc. Professor
Bengt Nordén, PI
1945
1971
Professor
Lisbeth Olsson, PI
1963
1994
Professor
Owe Orwar, PI
1964
1994
Professor
Anders Palmquist
1966
1997
Assoc. Professor
Sofia Svedhem
1970
2001
Ass. Professor (-May 2010)
Björn Åkerman, PI
1957
1990
Professor
#
Marie Curie Chair, Analytical Chemistry, Göteborg University
4
Karin Markides 511124-1021
SUPRA Centre
Appendix U
Experimental resources
Chalmers and Göteborg are very well equipped regarding experimental resources needed for
this project. Thanks to big instrument grants from the Swedish Research Council and several
private Foundations, about two major investments have been made every year for many years
at the Chemistry and Physics Departments. Today our resources include modern ultra-fast
laser spectroscopy, atomic-force and electron microscopy, mass spectrometry, advanced
microspectroscopy (SWEGENE Centre for Biophysical Imaging) and state-of-the-art facilities
for cleanroom micro/nanofabrication and characterisation (MC2) as well as nuclear magnetic
resonance (the National NMR centre being located in Gothenburg).
While we are well equipped with advanced physical instrumentation and theoretical tools
suitable for addressing our tasks, we anticipate encountering some severe challenges as our
molecular systems get more complicated. Our strategies as well as systems have a hierarchic
character: design and synthesis of modular molecular building blocks earmarked to selforganize into gradually bigger complexes, eventually reaching macroscopic dimensions. Here
thermodynamic and kinetic control of assembly and circumventing solubility problems will
provide major challenges as will, of course, the various advanced functionalities. Also
requirements of "addressability" and high structural fidelity will present challenges to the
design of building blocks and their later permanent fixations to each other.
We do not plan to use the Linnaeus grant directly for investments into experimental resources
or infrastructure. Instead we expect to continue to attract grants for renewing our
instrumentation from VR and private foundations (primarily the Knut and Alice Wallenberg
Foundation).
Organisation of the SUPRA Centre
The members of the group represent mutually complementary, cutting-edge competence
central for the project, all communicating in a common physical-chemical language. In
addition to the 10 PIs listed in the application, another 10 scientists (see Table 4) with longterm academic positions at Chalmers are engaged through collaborations. In addition, a
number of promising young scientists, directly or indirectly involved in the projects (as shown
by our citations), will be considered for recruitment to different positions. Among these
candidates we count: Jan Lagerwall, Gudrun Stengel, Kristine Kilså-Jensen, Elin Esbjörner,
Alexander Dmitriev, Pernilla Wittung, Tatsiana Lobovkina.
At present, the groups involved have 60 PhD students, 10 post-docs and 2 visiting professors
involved in this outstanding activity. The 20 involved scientists (Table 4) all work according
to goals set by themselves and it is our ambition that the situation will remain so. This is
necessary if scientific excellence in the group will be maintained and developed, in particular
for the younger members of the group. Nevertheless, the joint projects described in this
application will be promoted through the fruitful combination of complementary
competencies. Most of the PIs have already collaborated on topics of relevance for the
project, as documented by joint scientific publications. In order to further promote
interdisciplinary exchange, 10 PhD students will address related problems from different
angles. They will work in pairs, which, means e.g. a chemistry student collaborating with a
physics student on a specific problem. A unique feature of the program is the close contact
between theory, experiment and application. Seminars and PhD graduate courses to further
strengthen this feature will be at the core of our activities. The budget also includes resources
to invite guest lecturers and members of a scientific advisory board.
5
Karin Markides 511124-1021
SUPRA Centre
Appendix U
Rejuvenation - and retiring scientists
Ten years project time means that the current crew of senior scientists will be gradually
succeeded by younger ones. However, another urgent reason for renewal is the need to
respond to the incessant move of the research front. Thus, already from start we plan to recruit
(for three years) three to four postdoctoral research associates and to consider additional
possibilities for tenure track positions to connect with the project, in order to get young
scientists to bring new skills and new perspectives.
Leadership
A steering group of three persons will be responsible for policy, resource allocation and
reports to Chalmers and VR. For the first three-year period the group will consist of Linda
Gunnarsson, Bengt Nordén (chairman) and Lisbeth Olsson.
Bengt Nordén, will act as the coordinator for the centre and he has the appropriate experience
from leading several EU-projects and national research environments supported by the
Swedish strategic foundation (SSF). He is presently the chairman of the Council of the
European Research Councils Chemistry Committees (CERC3) and President of the Chemistry
Section of the Royal Swedish Academy of Science. Furthermore, Bengt Nordén has more
than 20 years experience as head and Chair of Physical Chemistry at Chalmers.
Relation to other existing and proposed Linnaeus programs
Our project would benefit synergistically from interactions with several research groups, both
in Sweden and abroad, including already established Linnaeus projects, such as the program
coordinated by Per Delsing, Chalmers (Engineering Quantum Systems) on electron transfer
and methods to contact individual molecules, as well as the program coordinated by Håkan
Wennerström, LU on theory and applications of molecular assembly. At Chalmers the
applications of our project and the Chalmers Soft Matter Centre (SOFT) have synergistic
potential although their foci are completely different. Three of the PIs are common in SOFT
and SUPRA but their contributions to the two programs will be radically different. In
SUPRA, Krister Holmberg, Fredrik Höök, and Mikael Käll provide competencies for
applications of several of the anticipated supramolecular structures whereas in the SOFT
program they provide competence on self organization and functionalization of soft matter.
Both programs will benefit from using their advanced experimental tools for imaging,
manipulation and characterization of soft matter systems.
Chalmers approach to stimulate the research environment of the SUPRA Centre
proposal and processes for support and promotion of the management
Since the proposal for the SUPRA Centre represents a combination of excellent researchers
from strong groups, the suggested research team will clearly position itself as one of the most
important scientific environments at Chalmers. Thereby it does not only correspond well to
Chalmers priorities – it actually constitutes an essential element in the research strategy.
Chalmers ensures that the group will get best possible conditions for their research to thrive in
the rich scientific setting available at the chemistry and physics departments. The groups
concerned have already successfully attracted funding from research councils and other vital
financial sources, which gives an advantage in Chalmers internal distribution system for the
governmental block grant for research. With a positive outcome of the current proposal, the
6
Karin Markides 511124-1021
SUPRA Centre
Appendix U
groups will benefit even further. In addition to the funding based on successful applications,
the proposal will be supported with another 1 MSEK per year, would it be funded within the
Linnaeus grant programme.
Perhaps more important to the success of the SUPRA Centre is the total scientific setting in
which it will be conducted. Chalmers has a considerable strength in areas related to the
proposal. Surrounding activities will contribute to, and benefit from, the strength concentrated
under the suggested Linnaeus grant recipient. Chalmers’ strategies will emphasize a culture of
openness, and will nurture this culture through priorities aimed to develop new interdisciplinary connections, supported by various incentives. As focal points for concentration of
research potential and renewal the SUPRA Centre will be in an excellent position to utilise
this emerging new culture of enhanced cooperation and boarder crossing research in the
coming years.
Chalmers whole range of prominent research is thus accessible for this new research
direction. This of course also includes Chalmers extensive external network for collaboration
with other academic institutions in Sweden and abroad, as well as with the corporate sector
and research institutes. Chalmers close cooperation with the Göteborg University is of special
importance and becomes inclusive in Chalmers development into new areas, where the
university has a long tradition.
Chalmers currently hosts the only Research Council financed Strong Research Environment
in the west of Sweden, the “Gothenburg Stochastic Centre”, and one Linnaeus initiative
“Engineering Quantum Systems” both supported by the Swedish Research Council. Chalmers
also hosts four new Vinn Excellence-centres of competence supported by VINNOVA
(Swedish Governmental Agency for Innovation Systems) and three Competence centres
financed by Energimyndigheten (Swedish Energy Agency). The latter are now running for a
second financial period following positive evaluations of the first period. The VinnExcellence centres are: the “GigaHz Centre”, the “Wingqvist laboratory - excellence centre
for efficient product realization”, the centre for “Supramolecular biomaterials – structure
dynamics and properties” and the “Chalmers Antenna Systems Excellence Centre” (CHASE).
Furthermore, SSF (Swedish Foundation for Strategic Research) is funding two strategic
research environments at Chalmers, the Gothenburg Mathematical Modelling Centre
(GMMC) and the Chalmers Research Centre on Microwave Antenna Systems
(CHARMANT). In addition, Lighthouse (marine technology area) and SAFER (vehicle and
traffic safety area) are two other major centres that have recently been established at Chalmers
with substantial support from VINNOVA together with industrial partners. Some of the
centres are operated jointly with Göteborg University.
All centres have become strong actors in their respective fields of research and innovation as
pointed out in several evaluations. The efficient Chalmers innovation system is beneficial for
the centres. Turning research and innovations into products and production systems are areas
of strength at Chalmers. This has also been concluded in different reviews, most lately in a
report concerning integration with society, made by the National Agency of Higher Education
in 2004.
Chalmers has thus developed a considerable experience in making major research efforts
successful. Chalmers will make the experience from earlier initiatives available throughout
the university. New centres will be able to draw from available practical conclusions on how
large funds can be used to obtain best achievements, and how input from different areas can
7
Karin Markides 511124-1021
SUPRA Centre
Appendix U
be coordinated. Annual meetings with the management of the larger funded research
environments at Chalmers is one way that will be used to disseminate these experiences.
The management of the SUPRA Centre will find support in professional administrative
functions both at departmental and university level. This includes financial administration,
staff matters, admission and support for PhD students and information, among other. An
efficient support structure allows the scientists to concentrate on the research activities.
Information officers will make the SUPRA Centre visible within Chalmers and beyond.
Systems for scientific information are in place, well developed and accessible.
As all significant and long term initiatives, a research environment funded under the Linnaeus
grant programme will be addressed in the annual assessment and planning process. This
ensures immediate attention from the top university management on the development of the
initiative. It also ensures appropriate conditions for the groups and departments involved.
Through the annual assessment and planning process, Linnaeus grants will be given due
attention in the annual report of the university.
Strategic priorities at Chalmers and its relation to the Linnaeus proposal: Centre for
Bio-inspired Supramolecular Function and Design ("SUPRA Centre")
Chalmers is currently in the middle of a strategic process, aiming at a new strategic plan from
January 1, 2008. The new plan has a more pronounced focus on sustainability and crossdisciplinary initiatives. The plan will be based on the overall vision “Creating a Sustainable
Society”. Chalmers is already gradually starting to work in a new way that reflects the role the
university intends to play in the future. A core element of the new strategy is increased
openness and support to inter-disciplinary activities that we are convinced will promote new
research connections and directions. By combining the strength of different research
environments at Chalmers, coupling profound scientific knowledge with an understanding of
complex systems, Chalmers will be able to take on more of the intricate challenges of modern
society and contribute to a sustainable future.
Chalmers ambition is to continue to build a strong base of research in the basic sciences,
Mathematics, Physics, and Chemistry and to combine this knowledge with more applied
engineering research areas, with a focus on expanding the current system boundaries and thus
addressing areas such as complex systems. The overall aim is to produce researchers and
engineers that can make a difference in society by using their knowledge, their toolboxes and
not least their spirit to solve the problems of tomorrow. To manage this, close cooperation
with other academic disciplines, society and industry as well as internationalization are still
cornerstones for Chalmers.
The proposed areas for Linnaeus support are selected with these aspects in mind. They have
been carefully assessed with respect to scientific standards, potential for success in their aim,
potential for renewal and for international reputation. The chosen areas contribute to essential
parts of Chalmers’ strategy, not least as they are working in the cooperative and including
way that will be an essential part of the strategies. The proposed areas are thus firmly
anchored in Chalmers’ long term development.
Chalmers has, for Swedish conditions, a unique possibility to receive support for its strategic
initiatives from the university’s owner; the Chalmers’ Foundation. So far the Chalmers’
8
Karin Markides 511124-1021
SUPRA Centre
Appendix U
Foundation has focused the support on the following areas: Microtechnology and
Nanoscience, Environmental Science/ Sustainability, Bioscience, New Materials and
Information Technology, with a number of application areas within each. During the last 10
years the Chalmers Foundation has given a total support of 197 MSEK for the MC2 clean
room, some 85 MSEK for the Environmental initiative and 150 MSEK for the Bio initiative.
Once successfully established, the research areas will have sufficient volume and strength to
attract new funding from external sources. The best example so far is Chalmers’
Environmental initiative. The environmental research at Chalmers has today significantly
higher total funding than it had during the years it was supported by Chalmers’ Foundation.
These examples are relevant for the proposal of the SUPRA Centre since they show how
Chalmers manages to turn a focussed funding into viable research activities with the ability to
find necessary continuous financing. It also shows the power of Chalmers’ Foundation to
promote initiatives that are considered important for the future. The proposed Linnaeus
initiative in Bio-inspired Supramolecular Function and Design is certainly of such
importance.
The role of the overall research strategy is mainly to identify strategically important areas and
to promote them. The detailed structure of the strategy develops as a consequence of
individual researchers’ initiatives, in cooperation with their colleagues, and their ability to
successfully compete for funding. The assessments made by the scientific community
ultimately define the strongest topics pursued at Chalmers, within the more broadly defined
strategies. Thus the research profile of Chalmers partially depends on how well the different
initiatives can compete for national and international funding.
This Linnaeus proposal is an example of an excellent research environment, within a strategic
area, focused on challenging tasks. It is designed to make Chalmers become one of the worlds
leading actors within the area. Such environments make Chalmers attractive for talented
young scientists, which is of crucial strategic importance for Chalmers. Recruitment of both
excellent faculty and promising young faculty are key factors for future competitiveness. For
this reason Chalmers has appointed a vice president dedicated to the issue of strategic
recruitments but also to development and promotion of existing faculty.
The proposal constitutes a well balanced combination of ongoing collaboration and a bold
attempt to move the research frontiers in new directions, based on commonly identified
challenges which unite the fields involved. In this particular example the development into
activities that will bring competences in the physics and chemistry departments together for
the proposed research will not be possible unless additional funding is provided. In the
process of selecting themes for the Linnaeus proposals the aspects worth developing beyond
ongoing cooperation between the groups have been further elaborated. The process of
developing new research directions will, regardless outcome of the current round of
proposals, become important for the future development of Chalmers.
From the identification process Chalmers has selected the SUPRA Centre for a Linnaeus
proposal since this area is strategically important and has proven to represent a strong
research approach. Here the molecular sciences at the interface between chemistry, physics
and biology offer unprecedented opportunities regarding theoretical and experimental
methodologies to match the challenge of how to produce new supramolecular systems and
how to control and exploit their properties for advanced technical purposes. This choice is
based on a firm belief that the research team has the ability to produce knowledge at the
research frontier today as well as in the future.
9
Karin Markides 511124-1021
SUPRA Centre
Appendix U
The development of campus is also strategically important and it has to be coordinated with
the overall strategies. Conscious re-localisation of departments at Chalmers has lead to
clustering of scientific areas which have further promoted cooperation. Most recently (2005),
the Department of Mathematical Sciences was moved to the heart of Chalmers’ campus, in
the vicinity of the other basic science departments. This has proven to be of great benefit in
creating a dynamic environment for most of Chalmers’ research areas.
The long-term financial support of a Linnaeus environment is advantageous. It would enable
the group to reach their goals at the same time as the challenges of unforeseeable results can
be followed up. They can mobilise their research efforts to move the frontiers in the
interdisciplinary area between molecular physics and physical chemistry. The potential for
progress can thereby be transformed into exciting and edge-cutting results. Ten years of
funding also gives the departments’ time to adapt to the Linnaeus environments and they will
become strong actors in the development of both Chalmers and the involved departments.
New clusters or chains of excellence are given time to emerge.
10
Kod
2007-14950-54750-89
Name of applicant
Markides, Karin
Date of birth
511124-1021
Title of research programme
Appendix V
Other information
VRAPS/VR-Direct bilaga 2004.Re
Vetenskapsrådet, SE-103 78 Stockholm, tel. +46 (0)8 546 44 000, [email protected]
Karin Markides 511124-1021
Supra Centre Chalmers
Appendix V
New direction for gender equality work at Chalmers
Increasing the gender equality at Chalmers is a question of improving Chalmers as a technical
university and as a place of work. In order to succeed in the competitive international arena,
Chalmers must employ the best researchers and be such a good place of work so that these
people will stay and enjoy work at Chalmers. One decisive part in that work is to take
advantage of the talent and resources of sexes.
Chalmers has an uneven gender distribution today, both among employees and students.
There are two risks with gender equality activities to which special attention must be paid at a
company with uneven gender distribution. Firstly, the under-represented sex can become
“invisible” if one does not keep on actualizing the question, and, secondly, the underrepresented sex is made visible and noticed in a distinct and problematical manner.
All work towards gender equality at Chalmers rests on three basic assumptions:
• that there is no difference between women and men when it comes to
talent within science and engineering1,
• that minorities are often exposed to negative stereotyping which
negatively affects their performance2 and
• that directed initiatives for stereotyped minorities can contribute to
strengthening the degree of stereotyping.2
Based on these assumptions, it follows that the gender equality work at Chalmers should not
consist of actions directed towards specific minority groups, but rather of broad initiatives from
which all can benefit, thereby also minorities, but without specifically pointing out these
minorities as a problem. People who belong to a supportive and comfortable group, not
grouped together due to any specific stereotype, perform better than people who have in some
way been stereotyped, even when specific actions are taken to help these people. Every gender
equality action at Chalmers should therefore, before being decided upon, be specifically
examined so that it will not contribute to a continued stereotyping.
Present situation and plans for gender equality within the SUPRA centre
In the Supra Centre two PIs out of ten are women (Table 1), while for the whole group the
ratio reaches 6 out of 20. In our view this increase reflects a slow but steady progress towards
a more natural distribution between the genders, caused by a similarly slow increase in the
number of women at the PhD, assistant and associate professor levels. At Chalmers (and
elsewhere) this trend is particularly strong in chemistry, probably because our three
undergraduate chemistry programs attract women to the extent that they make up 50% or so
of the rosters. Therefore, in a long-term perspective, the most productive gender-approach is
simply to stimulate qualified students to pursue academic careers. In this strategy we have an
advantage in having a high fraction of female PhD students in chemistry, because we know
from experience that they serve as excellent role-models for undergraduate female students.
We believe that patient adherence to this “bottom-up” approach is the best remedy for shifting
the present gender imbalance.
1
Janet Shibley and Marcia C. Linn, ”Gender Similarities in Mathematics and Science,” Science, 314, 599, 2006
Claude M. Steele, ”A Threat in the Air, How Stereotypes Shape Intellectual Identity and Performance”.
American Psychologist, 52, 613, 1997.
2
1
Karin Markides 511124-1021
Supra Centre Chalmers
Appendix V
The stimulating environment of the Supra Centre, with its focus on challenging scientific
issues, will be attractive for talented people at all stages in their academic careers. The Supra
initiative will enable M.Sc. students to become PhD students within the Centre, and secondly
will ensure that a high number of the graduated PhDs will be able to obtain post doctoral
positions within our international network. These long-term advantages will attract the most
talented undergraduate students, many of whom will be women why we are convinced that
our general efforts will promote a balance between the genders.
We will also take some more active measures to help tip the balance. Our first step is to forma
steering group with a gender distribution (two women and one man) intended to ensure that
the strategies outlined above are enforced in practice from the start. Secondly Within 5 years
two of the present PIs will retire, why it is noticeable that several of the associated scientists
are women with strong credentials to become PIs. In Appendix U we specify the resources
reserved for the promotion of several of the female participants from assistant professors to
tenured positions. This is a strategic effort to obtain more women as role models also at the
higher academic levels. The Table presents our goals for year five.
In our proposal we include a list of young and promising scientists (Alexander Dmitriev, Elin
Esbjörner Kristine Kilså-Jensen, Jan Lagerwall, Tatsiana Lobovkina, Gudrun Stengel,
Pernilla Wittung) that we would be ready to consider for the necessary rejuvenation. They are
directly or indirectly involved in the projects we propose (as shown by our citations). The
fraction of women in this group is large, and it is a reasonable estimate that by the fifth year
the number of involved female senior scientists in the SUPRA Centre is close to 50%.
Current members of the Supra Centre
Name
Age
Present position
Bo Albinsson, PI
44
Professor
Mats Andersson, PI
41
Professor
Ann-Sofie Cans
36
Ass. Professor (-May 2009)
Annika Enejder
38
Assoc. Professor
Andrew Ewing#
50
Professor
Linda Gunnarsson, PI
35
Ass. Professor (-Jan 2010)
Krister Holmberg, PI
61
Professor
Fredrik Höök, PI
41
Professor
Nina Kann
43
Assoc. Professor
Zoran Konkoli
41
Assoc. Professor
Mikael Käll, PI
44
Professor
Sven Larsson
66
Professor
Per Lincoln
49
Assoc. Professor
Jerker Mårtensson
42
Assoc. Professor
Bengt Nordén, PI
62
Professor
Lisbeth Olsson, PI
44
Professor
Owe Orwar, PI
43
Professor
Anders Palmquist
41
Assoc. Professor
Sofia Svedhem
37
Ass. Professor (-May 2010)
Björn Åkerman, PI
50
Professor
Plan/Goal for year 5
Associate Professor
Associate Professor
Retired
Professor, PI
Retired
Professor, PI
Retired
Associate professor
2
Karin Markides 511124-1021
Supra Centre Chalmers
Appendix V
English translation of popular description
Chemistry is the science of molecules, i.e. particles consisting of defined set of atoms that are held together
by strong bonds. A chemist may design and synthesize molecules with useful properties, e.g. a
pharmaceutical or a constituent of a new material. While chemists generally focus on how to make
molecules of such desired properties through control of their internal structures, it is also rewarding to
understand and exploit weak interactions between the molecules. Such inter-molecular interactions contain
the clues to the chemistry of life as they provide both the basis of recognition, necessary for spontaneous
formation of large structures with advanced functions, as well as the flexibility of closing and opening the
structures (assembly and disassembly) which are requirements for energy-driven motion, metabolism and
repair. An important example is the selective inter-molecular interactions (base stacking and base pairing)
which imprints the genetic code in the DNA molecule. This and other biological systems provide inspiring
examples how the collective action of many weak interactions can be exploited to spontaneously build up
"supramolecular structures", i.e. a well-defined structure of many molecules held together by the weak
forces. We intend to use synthetic DNA as one kind of building block with the DNA base recognition code
(adenine fitting only to thymine and guanine fitting only to cytosine) as a digital "address code" so that
different building blocks may be sent into the construct to find their specific target positions in a correct
way. Thus, supramolecular structures with variable shapes, sizes and properties can, in principle, be
designed at will and be assembled using rather simple encoding algorithms and short DNA sequences
programmed to fit like pieces in a jig-saw puzzle. This is just one example inspired by biological structures
that we intend to pursue. Another is mimicking the lipid membranes that are the walls of cells, into whose
surfaces various functional molecules can be inserted to form another kind of supramolecular system.
To illustrate how supramolecular structures could be exploited, we may consider the development of
digital electronics, present in everyday devices such as mobile phones, video screens and GPS navigation
system. This development is dependent on a miniaturization of electronics which to date has been
accomplished by lithographic techniques, reaching not far below 100 nm. A new kind of technology that
allows miniaturization below the present limits will soon be needed, and our supramolecules, whose
dimensions typically span between 1 and 100 nm, may close this gap between the nm size molecules and
the lithographic dimensions, and thus be of great strategic value. This is just one example how the
supramolecular structures may be used practically: nm-size molecular transistors may for instance be put in
position on a nano-chip by an appropriate DNA address. Other applications of supramolecular assemblies
include information storage, solar light energy conversion and chemical nano-scale reactors, possibilities
represented by various research projects in the proposal. However, our main goal is not to develop new
technology but to study and understand at a fundamental level the interactions and phenomena on which
new applications may be based. One question we want to address is whether recognition between
molecules must be based on how well they fit, like a key in a lock, when bound together, or if other
properties may provide alternative mechanisms for recognition, such as how fast the molecules can move
relative to each other during the binding reaction.
3
Kod
Dnr
2007-14950-54750-89
2007 -
Co-ordinator/Repr.of University
Markides, Karin
Date of birth
Reg date
511124-1021
2007-11-06 11:54:53
Title of research programme
*Linnéstöd och Berzelius Center
VR
Chalmers University of Technology
2008-06-01 -- 2017-12-31
VR-N
2008
7500
Appendix S
*Naturvetenskap
2009
7500
2010
7500
2011
7500
Signatures
2012
2013
2014
2015
2016
2017
7500
7500
7500
7500
7500
7500
Repr.of University
Date
Clarifi cation of signature
Telephone
2018
Vetenskapsrådets noteringar
Kod
VRAPS/VR-Direct bilaga 2004.Ss
Vetenskapsrådet, SE-103 78 Stockholm, tel. +46 (0)8 546 44 000, [email protected]