Antibodies against modified nucleic acids
For Research Use Only
- For detection of modified DNA/RNA bases and nucleosides!
Some nucleobases and backbone sugar in DNA and RNA nucleosides are naturally modified in vivo and the
modifications epigenetically contribute to the regulation of gene expression. Typical examples include repression
of transcription by DNA methylation and regulation of translation by tRNA modification. It is reported that the
modification state changes in DNA and/or RNA nucleosides are closely related to embryonic development, circadian
rhythm and various disorders such as cancer.
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Anti-1-methyladenosine (m1A) mAb
1-methyladenosine (m1A) is one of the modified nucleosides present in tRNA and rRNA. It is reported that the levels of 1-methyladenosine in
serum and urine are elevated in cancer patients and under certain stresses such as ischemia and oxidative stress.
■ Immunohistochemistry
■ Immunocytochemistry
Tissue: ischemic mouse liver*
Cells: HeLa
Green: Anti-1-methyladenosine (m1A) mAb
Blue: Hoechst33342
■ RNA immunoprecipitation
Mouse IgG2b
RNA intensity
Anti-1-methyladenosine
(m1A)mAb
Average of the RNA Quantity (n=2)
Antibody
RNA (ng)
Mouse IgG2b
Anti-1-methyladenosine (m1A) mAb
40.0
320.0
Nucleotide length
Code.
D345-3
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Product name
Clone
1
Anti-1-methyladenosine (m A) mAb
AMA-2
Isotype
Mouse IgG2bκ
Size
100 µg/100 µL
Application
IH / IC / RNA-IP
Cross-reactivity
1
m A (RNA), m1dA (DNA)
Anti-5-methylcytidine (m5C) mAb
5-methylcytidine (m5C) is one of the modified nucleosides present in tRNA, rRNA, mRNA and lncRNA. It is reported that the levels of
5-metylcytidine in serum and urine are elevated in patients with various disorders such as cancer. This antibody can be used to detect
5-methylcytosine (5mC) in DNA, as well as m5C in RNA.
■ Immunohistochemistry
Tissue: ischemic mouse liver*
5-methylcytidine (m5C)
Mouse IgG2a (isotype control)
Heat-treated (for nuclear staining)
Tissue: Mouse testis
*:The mouse model for liver ischemia (sections of liver) was provided by Dr. Takaaki Abe from the Division of Medical Science at Tohoku University Graduate School of Biomedical
Engineering, Department of Clinical Biology and Hormonal Regulation, Tohoku University Graduate School of Medicine.
http://ruo.mbl.co.jp/
■ Immunocytochemistry
HCl-untreated
HCl-treated
Cells: HeLa
Green: Anti-5-methylcytidine (m5C) mAb
Blue: Hoechst33342/DAPI
Code.
D346-3
Product name
Clone
Isotype
Size
Application
Cross-reactivity
Anti-5-methylcytidine (m5C) mAb
FMC-9
Mouse IgG2aλ
100 µg/100 µL
IH/ IC/ Dot blot*/ DNA-IP*
m5C(RNA), m5dC (DNA), 5mC
* Data are available on the website.
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Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
Small nuclear RNA (snRNA) is known to be involved in pre-mRNA splicing as an RNA component of snRNP. 2,2,7-trimethylguanosine (m3G/TMG) is a unique
modified nucleoside at the 5’-cap site of U snRNAs except for U6 snRNA.
This antibody is available for enrichment or elimination of the snRNA and snoRNA which possess m3G/TMG cap structure at its 5’-end in total RNA by RNA-IP.
■ RNA immunoprecipitation
Input total RNA
(3)
RNA intensity
snRNA/snoRNA
Mouse IgG2b
Anti-2,2,7-trimethylguanosine
(m3G/TMG) mAb
(2)
(1)
Nucleotide length
(1) Small RNA (tRNA, snRNA, snoRNA, etc.)
(2) 18S rRNA
(3) 28S rRNA
■ Immunocytochemistry
Average of the RNA Quantity (n=2)
Antibody
RNA (ng)
Mouse IgG2b
66.6
Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
887.6
Code.
RN011M
Cells: HeLa
Red: Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
Cyan: DAPI
Product name
Clone
Isotype
Size
Application
Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
C1-36
Mouse IgG2bκ
200 µg/200 µL
IC / RNA-IP / RIP*
Cross-reactivity
m3G (RNA)
* Data are available on the website.
Related products
Code.
Product name
Clone
Isotype
Size
Application
PM077
Anti-5-hydroxymethylcytosine (5hmC) pAb
Polyclonal
Rabbit Ig (aff.)
100 µL
Dot blot / DNA-IP / IH
Cross-reactivity
MI-11-3
Anti-Bromodeoxyuridine mAb
2B1
Mouse IgG1
100 µg/100 µL
IP / FCM / IC* / IH
BrdU (DNA), BrU (RNA)
MI-11-5
Anti-Bromodeoxyuridine mAb-PE
2B1
Mouse IgG1
1 mL (50 tests)
FCM
BrdU (DNA), BrU (RNA)
5hmC (DNA)
* Reported in articles.
Application: IP: Immunoprecipitation, RIP: RNP Immunoprecipitation, IC: Immunocytochemistry, IH: Immunohistochemistry
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