Biology Leaving Certificate Higher Level
Past Exam Questions on:
Enzymes
Q 9 2013
Q9 2012
Q14 2011
Q14 2010
Q9 2009
Q 9 2008
Q9 (a)
What is meant by an enzyme’s optimum pH?
What is a denatured enzyme?
(b)
7.
In the course of your studies you investigated the effect of denaturation by heat application on the
activity of an enzyme.
(i)
Name the enzyme that you used
(ii)
W hat substr ate did yo u use?
(iii) Describe how you carried out the investigation. In your answer you must refer to the way
that you measured the enzyme’s activity
(iv) .................................................................................................. State the
results that you obtained.
Q7 2007
(a)
(b)
What is meant by an enzyme? .....................................................................................................
Give an example of a protein that has a structural role. ....................................................
Answer the following questions in relation to an investigation that you carried out to determine the
effect of temperature on enzyme action.
(i)
Name the enzyme that you used. .......................................................
(ii)
Name the substrate of the enzyme. .......................................................
(iii) State one factor that you kept constant during the investigation..................................
(iv)
How did you keep this factor constant?
( v)
H ow di d yo u var y the tem per atur e? ...................................................
(vi)
How did you measure the rate of activity of the enzyme? ...................................
(v)
What was the result of your investigation?
Q 11(c) 2007
Enzymes can be immobilised and then used in bioprocessing.
(i)
What is meant by immobilisation?
(ii)
Name a substance that is used to immobilise enzymes.
(iii)
Give two advantages of using immobilised enzymes.
(iv)
Give one application of a named immobilised enzyme. In your answer, refer to substrate,
enzyme and product.
(24)
Q3 2006
The graph shows how the rate of reaction of a carbohydrate-digesting enzyme in the human alimentary canal
varies with pH.
(a)
Name a carbohydrate-digesting enzyme in the human alimentary canal .................
(b)
Where in the alimentary canal does this enzyme act? .........................................
(c)
State the enzyme’s pr oduct (s) .............................................................
(d)
What is the pH at A? ............................. .
(e)
A is said to be the enzyme’s ................................ pH
(f)
Suggest a temperature at which human enzymes work best .......................................
(g)
What term best describes the shape of an enzyme?
Q7 2005
.
(a)
(b)
Immobilised enzymes are sometimes used in bioreactors.
( i)
W h a t i s a b i o r e a ct o r ?
(ii)
State one advantage of using an immobilised enzyme in a bioreactor.
Answer the following questions in relation to an experiment that you carried out to immobilise
an enzyme and use that immobilised enzyme.
(i)
Name the enzyme that you used ......................................
(iii)
Draw a labelled diagram of the apparatus that you used to immobilise the enzyme.
(iv)
Describe how you used this apparatus to immobilise the enzyme. In your answer name the
solutions that you used and explain their purpose.
(v)
Describe briefly how you used the immobilised enzyme.