CHANGES IN CHINESE HAIR GROWTH ALONG A FULL YEAR C. Liu*, J. Yang*, L. Qu*, M. Gu*, Y. Liu*, J. Gao*, C. Collaudin† and G. Loussouarn† *L’Oreal R & I, Jinyu Rd No. 550, Shanghai, 201206, China and †L’Or_eal R & I, 20120611-13 rue Dora Maar, Saint-Ouen, 93400, France INTRODUCTION ABSTRACT As a physical fibre, the human hair shows a large diversity in shape, colour, length, diameter, ellipticity. . .with ethnics, age or fashion. As a biological end product of the hair follicle ‘machinery’, it obeys to successive cycles of growth, arrest, shedding and a further regrowth. Aim of the study was to confirm the existence of seasonal hair growth cycle among Chinese subjects and objectivize the seasonal effect of hair loss. METHODS 1 Protocol At approximately comparable days within months, subjects came into the laboratories for a consecutive 2-day presence (D0 and D2) of about an hour each, due to the inherent practical aspects of the widely used phototrichogram technique. Visits were repeated monthly over a 1-year period. 2 The phototrichogram technique At D0, a small scalp area (1.5 cm by 1 cm) of the vertex region was shaved. A first photograph was then immediately taken under standardized lighting conditions. The Canfield Epiflash objective, mounted on a Nikon D90, allows being gently pressed onto the scalp area. Therefore recording pictures at a constant distance between scalp and camera for all subjects. The same procedure was repeated 2 days later (D2). 3 Image analysis For each month and subject, these two consecutive digital photographs were processed through image analysis, using an L’Oreal dedicated software. Globally, the latter allows a precise reframing of the two pictures, an automatic detection, counting all hairs and comparing the lengths of individual hairs. This procedure allows, at D2, hairs of a longer size at D2 (anagen, growth) or of a same length (telogen, no growth) to be detected and counted. These rapid computations provide the total number of hairs, within the same recording frame, their density (N/cm2), A% (total grown hairs/N) and T% (total not grown hairs/N). Growth rate comes as a logical step, by calculating the individual difference in length over a 2-day period of anagen hairs. Lastly, a manual procedure allows the diameter of a given hair to be recorded by selecting two regions along its shaft, leading to an average width (in lm) from an automatic calibration referred here as its ‘diameter’. RESULTS 1 Global data Table below summarizes the ranges of major hair growth parameters of Chinese subjects observed during 13 successive months, the values of which clearly correspond to healthy head of hair, free from alopecia. 2 Changes in telogen values, anagen values, growth rates, hair density and diameter over a one-year period With regard to T%, Fig. (a) below illustrates the average changes in T% along a full year for Chinese subjects, indicating a higher value in September, as compared to other months. Whereas, Fig. (b) illustrates the average changes in A% along a full year, indicating a lower value in September. Below illustrate a rather constant value of hair growth rate (375–425 lm/day range) along the year. 3 Relation between growth rates and diameters As all analysed hairs were not individually identified by these two criteria (diameters being determined at random), classes of diameter were created (70–80 lm, 80–90 lm etc. . .) allowing to calculate, for each class, an average growth rate being calculated. Figure below illustrates, both genders included, a linear and highly significant correlation between both parameters, that is the thicker the hair, the faster growing. This confirms previous data obtained on individualized Asian (straight shaped) hairs. With regard to hair density (below figure), Chinese women present slightly higher values than men by about 10 hairs/cm2 (approx. 225 vs. 215, respectively). However, hair density of both genders do not show significant changes along the year, the average monthly values oscillating around 220/cm2. Gender effect on hair density for Chinese population 240 Hair density (Hair cm-2) 235 230 225 220 215 210 205 Male 200 Female 195 Jan Feb Mar Apr May Jun Jul Aug Sept Oct Nov Dec Jan Month (2012-2013) CONCLUSION The data collected monthly over a full year globally confirm the existence of a seasonal hair growth cycle (increased T% at late summer) among Chinese subjects. As compared to other ethnics, Chinese hairs appear thicker and of a faster growth, which is in agreement with previous findings. In addition, this data confirm the strong link between hair growth and hair thickness by analysing about 1600 individualized straight hairs from Asian subjects. With regard to hair diameter, below show rather constant average values along the full year.
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