queensland museum learning Student task sheet Mangrove Challenge Raising wrigglers If you live in a coastal area of Queensland, you may have a problem with mosquitoes every summer. But by understanding their life cycle, and how it can be interrupted, you can develop some strategies to control mosquito numbers. What you do: Mosquito eggs will hatch one or two days after they are laid on the water surface. In a few more days the larvae or wrigglers can be seen, and easily taken from the water using a net. In this activity you can ‘raise’ mosquito eggs and larvae, then experiment to find out how to stop them developing. This activity works best in the warmer months. Fill a bucket(s) with water. Leave in a shady place. Background activity: >> Male and female mosquitoes have different ways of life and adaptations. Find out about these differences. >> Three species of mosquitoes are considered pest species in Queensland: Aedes vigilax, Culex annulirostris and Aedes notoscriptus. Find out if any species are considered pests in your area. Do they transmit diseases? How serious are these diseases to human or animal health? Leave for one week. Use a net or strainer to harvest. Growing larvae Observe with a hand lens or low power objecttive lens of a microscope. Care: You should consider all mosquitoes dangerous. Avoid being bitten. What you need (for a class): >> buckets or containers to hold water >> pond water, rain water or tap water, shaken and left to stand overnight to remove chlorine >> handfuls of dried gum leaves added to tap water to provide organic matter for wrigglers >> aquarium net or strainer You can increase the number of wrigglers or eggs, if you: >> use black or dark-coloured buckets >> put strips of filter or blotting paper on the inside of the bucket at water level to provide a surface for egg laying >> leave the eggs for 8–10 days in a plastic bag, then flood with water — they hatch overnight. About wrigglers The wrigglers of Aedes vigilax are shown here. Their heads are down as they breathe through a siphon — an extension of the last abdominal segment — at the water’s surface. They move or ‘wriggle’ by contracting and relaxing their segmented bodies. The wrigglers of the Anopheles mosquito, which carries the fatal disease malaria, lack a siphon; instead they hold their bodies horizontally along the surface and take air through holes in their abdomens. QIMR queensland museum | po box 3300 | south brisbane bc | queensland 4101 | australia © Queensland Museum 2007 Raising wrigglers: Experiments with eggs or wrigglers In this commercial mosquito breeder, larvae can develop in pond water. After a short while, they pupate, or form a pupa or case. Pupae breathe at the surface and do not feed, in two or three days, adult mosquitoes will emerge from this case. They can be observed in the top part of the breeder. DO NOT ALLOW THESE MOSQUITOES TO ESCAPE. To kill the adults, place the breeder in the freezer for about half an hour, then wash with warm water and detergent. Typically, within two weeks of hatching, wrigglers will moult four times, and then pupate. If you want to experiment with wrigglers, you need to harvest them as soon as they can be seen. In this activity you will investigate factors that affect wriggler survival or egg laying, to determine ways to control breeding. To do this, you will need to plan your experiment as a Controlled experiment; see Science Skills. The following variables may affect mosquito breeding and development: >> surface chemicals such as kerosene or detergent >> temperature >> water movement >> vegetation cover >> depth >> other treatments such as salt. Research the effects of these variables on mosquito breeding. A useful source of information can be found at: <http://medent.usyd.edu.au/>. One of these variables, or one of your own, could be manipulated in your investigation. Evaluation: Make your own mosquito breeder: You can make this mosquito breeder from two one-litre soft drink bottles. First form the sides of the breeder. To do this, cut the neck off both bottles using scissors, then the base off one of the bottles. Next, attach one of the bottle necks with sticky tape inside the breeder sides, as shown. Then cut four slits in the side of the bottom breeder with scissors, and force the parts together. Finally, cover the top with a circle of muslin or voile, using a rubber band. 1. A well-designed and implemented investigation improves the validity of your results. Your investigation should have a control so that you can tell if a variable has affected either wriggler development or egg laying. How can you tell if a factor has affected the development of the wrigglers? How do you gauge the success of the treatments? 2. Comment on the effectiveness of factors that appear to affect wriggler development. Do any of them cause environmental problems? 3. Visit a local wetland area, and observe potential mosquito-breeding sites. 4. Contact your local council to find out what mosquito sprays are being used in your local area. Investigate the effects on the environment of widely used insecticides such as the organophosphorus-based temephos. Write a report on your findings. queensland museum | po box 3300 | south brisbane bc | queensland 4101 | australia © Queensland Museum 2007 2/2
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