accurate

accurate
protein analysis
Thermo Scientific Pierce Protein Ladders help determine molecular weight using colorimetric,
fluorescence or chemiluminescent detection. The Prestained Protein Ladders are recommended
for approximate determination of molecular weight, monitoring the progress of the electrophoresis run and the efficiency of the protein transfer to the membrane during Western
blotting procedures. For precise determination of molecular weights of target proteins in any
buffer system, we recommend using our unstained protein ladders. Our protein ladders
are manufactured in a quality-controlled environment that exceeds industry standards to
provide the highest quality, reliability and lot-to-lot consistency.
PageRuler™ Prestained
Protein Ladder
PageRuler Plus Prestained
Protein Ladder
Spectra™ Multicolor Broad
Range Protein Ladder
Spectra Multicolor
High Range Protein Ladder
kDa
kDa
kDa
kDa
kDa
– ~140 –
– ~300 –
– ~250 –
– ~40 –
– ~25 –
– ~100 –
– ~180 –
~55
– ~50 –
– ~35 –
~35
– ~40 –
– ~35 –
– ~25 –
~25
– ~25 –
– ~15 –
~15
– ~15 –
–
–
–
–
–
–
–
–
– 40 –
Pierce® Unstained Protein
Molecular Weight Marker
Part No. 26610
kDa
– 116.0
Gel
– 10 –
Blot
– 15 –
Part No. 26637
kDa
kDa
– 250
– 150
– 250
– 200
– 100
– 150
– 70
– 50
– 40
– 120
Part No. 26612
Part No. 26632
kDa
– 100
–
–
–
–
150
Part No. 26635
kDa
– ~120
– ~ 85
– 130 –
Part No. 84785, 84786
kDa
100
80
60
– 70 –
– 55 –
– 43 –
– 15 –
– 10 –
40
30
Gel
– 34 –
– 26 –
50
Near-IR Imager
Visible Light
– ~20
Gel
Gel
– 18.4
– 14.4
3.4
– 250 –
kDa
– ~25
5
–
SuperSignal® Molecular
Weight Protein Ladder
– ~35
– 25.0
–
PageRuler Prestained
NIR Protein Ladder
– ~50
– 35.0
30
25
20
15
– 10
– 70
– 60
– 95 –
– 45.0
– 1.7 –
PageRuler Unstained
Low Range Protein Ladder
– 100
– 85
– 15
– 10
– 5
Pierce Prestained Protein
Molecular Weight Marker
– 66.2
– ~40 –
PageRuler Unstained High
Range Protein Ladder
– 20
– 4.6 –
– ~50 –
– ~10 –
– 30
– 30 –
– 25 –
– 20 –
– ~70 –
Gel
200
150
120
100
85
70
60
50
Part No. 26630
– ~10 –
– ~100 –
Gel
–
–
–
–
–
–
–
–
Blot
PageRuler Unstained
Broad Range Protein Ladder
PageRuler Unstained
Protein Ladder
Part No. 26614
kDa
~10
Gel
Blot
– ~10 –
Gel
– ~40 –
– ~15 –
– ~130 –
Blot
~70
– ~55 –
Gel
– ~70 –
20
thermoscientific.com/pierce
© 2012 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only.
All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
Life Science Research
Africa/Belgium/Europe/Middle East
+32 53 85 71 84
France 0 800 50 82 15
Germany 0228 9125650
Netherlands 076 50 31 880
Switzerland 0800 56 31 40
UK 0800 252 185
1602453 05/12 Printed in the U.S.
Email: [email protected]
thermoscientific.com/perbio
For other regions, visit
thermoscientific.com/piercedistributors
USA +815-968-0747 or +800-874-3723
Customer Assistance
E-mail:[email protected]
thermoscientific.com/pierce
Blot
~100
– ~70 –
Part No. 26628
Gel
~130
– ~100 –
Gel
– ~260 –
~250
– ~130 –
Part No. 26625
Gel
– ~170 –
Part No. 26623, 26634
X-ray film
Part No. 26619, 26620
Blot
Part No. 26616, 26617
Spectra Multicolor Low
Range Protein Ladder
accurate
nucleic acid analysis
Thermo Scientific RNA and DNA Ladders provide accurate analysis of nucleic acid
in both agarose and polyacrylamide gels. Thermo Scientific DNA Ladders are made of
chromatography-purified individual DNA fragments manufactured using a proprietary
technology for preparation of pharmaceutical-grade plasmid DNA. Thermo Scientific RNA
Ladders are mixtures of chromatography-purified RNA transcripts that form sharp bands of
uniform intensity and are ideal for RNA sizing on both native and denaturing gels. Our ladders
and markers are manufactured in a quality-controlled environment that exceeds industry
standards to provide the highest quality, reliability and lot-to-lot consistency.
GeneRuler 1 kb
DNA Ladder
GeneRuler 1 kb Plus
DNA Ladder
SM0311/2/3*
GeneRuler 100 bp
DNA Ladder
SM0241/2/3*
SM1331/2/3*
bp
bp
10000
8000
6000
5000
4000
3500
3000
2500
2000
1500
20000
10000
7000
5000
4000
3000
1000
750
1000
700
500
400
300
200
75
2000
1500
500
250
GeneRuler Ultra Low Range
DNA Ladder
bp
50
35
25
20
15
200
150
bp
1000
900
800
700
600
500
400
300
3000
2000
1500
1200
1000
900
800
700
600
500
400
SM1233*
GeneRuler High Range
DNA Ladder
SM1351/2/3*
RiboRuler Low Range
RNA Ladder
SM1831/3*
bp
80
80
80
20
20
20
20
10
80
124
20
31
50
RiboRuler High Range
RNA Ladder
SM1821/3*
bases
bases
50
48502
24508
20555
17000
15258
13825
12119
25
10171
FastRuler Low Range
DNA Ladder
50
20
5000 80
20
2000 80
20
850
80
20
400
80
20
100
80
20
* Catalog numbers ending in 3 are ready-to-use, premixed with loading dye.
© 2012 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only.
All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
North America 800 340 9026
Europe 00800 222 00 888
[email protected]
[email protected]
200
SM1123*
bp ng/20 µl ng/5 µl
thermoscientific.com/ladders
500
FastRuler High Range
DNA Ladder
SM1113*
bpng/20 µl ng/5 µl
1500 80
20
850 80
20
400 80
20
200 80
20
80
2000
1500
1000
100
FastRuler Middle Range
DNA Ladder
SM1103*
4000
3000
1000
800
600
400
300
200
8 min
1602453 06/12 Printed in the U.S.
1000
900
800
700
600
500
400
300
250
200
150
100
6000
bpng/20 µl ng/5 µl
200
100
50
bp
100
100
75
FastRuler Ultra Low Range
DNA Ladder
SM0371/2/3*
200
700
500
400
300
10
GeneRuler 50 bp
DNA Ladder
300
100
SM1191/2/3*
bp
300
200
150
100
75
SM0321/2/3*
bp
200
GeneRuler Low Range
DNA Ladder
SM1211/2/3*
GeneRuler 100 bp Plus
DNA Ladder
bpng/20 µl ng/5 µl
10000
4000
2000
1000
500
80
80
80
80
80
20
20
20
20
20