The Lipid a
and Lipid
Metabolite
b li e Analysis
l i
Core Facility
Serviices
Rates
Samp
ples
The Core Facility is a joint initiative of the G
Group on the Molecular and Cell Biology of Lipids (MCBL) and the Cardiovascular Rese
earch Group (CVRG) with support from the Mazankowski Alberta Heart Institute (MA
AHI) and the Women and Children’s Health Research Instiitute (WCHRI)
Services Provided by the Core Faacility
Currently available:
•
•
•
•
•
•
•
•
•
•
•
•
FPLC lipoprotein profiling from vvarious sources including plasma, cell culture medium, and isolated inttracellular compartments (cholesterol / triglyceride / phospholipid
gy
p p
p p
profiles))
FPLC separation and purification
n of lipoprotein classes and subclasses including VLDL, LDL, and HDL
HPLC analysis and quantitation of phospholipid
o
classes from various sources including tissue, plasma, and cell extracts
HPLC analysis and quantitation of bile acids from various sources including o
isolated bile
HPLC analysis and quantitation of nucleotides including xanthine, o
hypoxanthine, inosine, IMP, NAD
D, adenosine, AMP, ADP, ATP, and GTP
HPLC analysis and quantitation of short and long chain CoAs
o
including free CoA and acetyl, malonyl, succinyyl, palmitoyl, stearoyl, and oleoyl CoAs
HPLC analysis and quantitation
l
d
of
of ceramides
d
GC analysis and quantitation of ttotal lipids
GC analysis and quantitation of ffatty acid chain length and saturation
customized HPLC or FPLC protein purification*
customized HPLC or FPLC lipid analysis*
customized analysis of other mo
t i d
l i f th
olecules and metabolites*
l l
d
t b lit *
Available in the near future:
•HPLC analysis and quantitation of to
otal lipid classes from various sources
•customized metabolite analysis usin
•customized metabolite analysis usin
ng the Luminex system
ng the Luminex
•mass spectrometry (alone or coupleed to HPLC separation)
*customized services will involve disccussion with clients regarding materials and methods required for analysis
Please email Audric Moses (audric.m
[email protected]) for current information on services, rates, and fo
or any other inquiries. Alternatively, you may phone the lab at 492‐5648.
FPLC separation of lipoprotein classes
(cholesterol profile)
Column: Superose 6 HR 10/30
Dimensions: 10 x 300mm
Mobile Phase: 50mM NaCl
Flow rate: 0.4 mL/min.
Reagent: total cholesterol
@
Detection: vis. @ 500nm
Sample: pure mouse plasma
absorbance
e units
60
0
CETP/apoB transgenic
wild-type
ild t
HDL
cholesterol
profile
40
0
LDL
20
0
HDL
VLDL
0
FPLC separation of lipoprotein classes
(triglyceride profile)
absorrbance units
60
Column: Superose 6 HR 10/30
Dimensions: 10 x 300mm
Mobile Phase: 50mM NaCl
Flow rate: 0.4 mL/min.
Reagent: triglyceride
Detection: vis. @ 500nm
Sample: pure mouse plasma
CETP/apoB transgenic
wild-type
triglyceride
profile
VLDL
4
40
LDL
20
HDL
HDL
0
HPLC separation of phospholipids
Column: Onyx monolithic silica
Column:
Onyx monolithic silica
Dimensions: 4.6 x 100mm
Mobile Phase: IPA/hexane/water
Flow rate: 1.5 mL/min.
Detection: ELSD
Sample: human liver extract
HPLC separation of bile acids
Column: Luna C18
Dimensions: 4.6 x 250mm
/
Mobile Phase: MeOH/CHCN
3/
water/HOAc/NH4OH Flow rate: 1.0 mL/min.
Detection: ELSD
Sample: commercial standards
GC separation of total lipids
FC
Column: Zebron ZB‐5
Column:
Zebron ZB 5
Dimensions: 15m x 0.32mm
Mobile Phase: helium
Flow rate: 16.9 mL/min.
Detection: Flame‐Ionization
Sample: mouse liver extract
TG C57
TG C55
TG C553
TG C551
CE C445
CE C443
PL C39
PL C41
STD)
TD (IS
MG C21
PL C35
PL C37
MG C19
MG: monoacylglycerol
FC: free cholesterol
TD: internal standard
PL: phospholipid
CE: cholesteryl ester
TG: triacylglycerol
GC separation of fatty acids
C24:0
C22:6
C20:4
C20:5
C22:0
C20:0
C18:3
C18:0
C14:0
C16:0
C18:4
C22:1
C17:0 (ISTD)
C
C18:2
C18:1
Column: J&W DB‐23
Dimensions: 30m x 0.25mm
Mobile Phase: helium
Flow rate: 6.4 mL/min.
Detection: Flame‐Ionization
Sample: commercial standards
Introductory rates
analysis type
price per sample
FPLC lipoprotein profile cholesterol
FPLC lipoprotein profile triglyceriide
FPLC lipoprotein profile phospho
olipids
FPLC non‐destructive separation and collection of
lipoprotein classes
HPLC phospholipids
HPLC bile acids
HPLC bile acids
HPLC nucleotides
HPLC short or long chain CoAs
HPLC ceramides
HPLC total lipids
GC t t l li id
GC total lipids
GC fatty acids
Luminex
Mass spectrometry
LC‐MS
customized analyses
d when submitting >10 samples
discounts may be requested
$30
$40
$50
$20
$30
$30
$30
$30
$30
$30
$25
$25
call
call
call
call