1592. Cyanobacteria Medium BG11 ‐ Volume
[mL/L]
10
Stock Solutions
[g/L]
7.5
CaCl2 ∙ 2H2O
10
3.6
Na3‐citrate 10
0.6
Na2‐EDTA ∙ 2H2O
10
0.1
MgSO4 ∙ 7H2O
Trace metal mix
1
‐
Adjust to 990 mL with de‐ionized or distilled water and autoclave.
After cooling, add the following filter components:
BG11‐Mix
Volume
[mL/L]
2
Stock Solutions
[g/L]
‐
Vitamin B12
2
0.01
Stock solutions
BG11‐Mix [g/L]
K2HPO4 ∙ 3H2O
20
Na2CO3
10
Fe‐NH4‐citrate
3
Trace metal mix
[g/L]
[ml/L]
H3BO3
2.9
<‐
MnCl2 ∙ 4H2O
1.81
<‐
ZnSO4 ∙ 7H2O
0.22
<‐
Na2MoO4 ∙ 2H2O
0.39
<‐
CuSO4 ∙ 5H2O ->
10 ml of a 0.8 g/100 ml stock solution
Co(NO3)2 ∙ 6H2O
->
10 ml of a 0.5 g/100 ml stock solution