!
w
e
N
!!
aatt
m
rrm
FFoo 99
ee
aarr 88 &&
ddww ss
aarr ggee
HH ppaa
SS
M sseeee
//M
LLCC
Versatile Packings for a
Wide Range of Applications
Acid and Base Deactivated
for Symmetrical Peaks
Stable Bonded Phases for
Long Column Life
A Large Selection of Hardware
Formats
Bulletin #458
Versatile Packings for a Wide Range of Applications
What are You Looking for in an HPLC Column?
Alltech’s Alltima™ HPLC Columns will satisfy the requirement of today’s applications!
• Large Selection of Mobile Phase Chemistries, Particle Sizes, Porosities, and Column Formats
• Extensive Collection of Published Applications
• Demonstrated Stability for Acids, Bases, and Neutrals in a Wide Range of Mobile Phases
Alltima’s performance and versatility are exemplified by the following applications.
Antibacterials
Natural Products
8764
1. Oxytetracycline Dihydrate
2. Tetracycline HCL
3. Meclocycline Sulfosalicylate Salt
3
1.
2.
3.
4.
5.
6.
2
1
1
2
3
8887
Naringin
Neohesperidin
Myricetin
Quercetin
Naringenin
Kaempferol
5 6
4
0
2
4
6
8
0
10 Min.
Column:
Alltima™ C18, 3µm, 100 x 4.6mm
Mobile Phase: A: 50mM KH2PO4, pH 3.0 B: CH3CN
Gradient
Time: 0 10
%B:
15 40
Flowrate:
1.0mL/min
Detector:
UV at 254nm
Antihistamines
5
15 Min.
10
Alltima™ C18, 3µm, 100 x 4.6mm
A: 50mM KH2PO4, pH 2.5 B: CH3CN
Time: 0 15
%B:
20 40
1.0mL/min
UV at 280nm
Column:
Mobile Phase:
Gradient:
Flowrate:
Detector:
Flavonoids
7232
4
9271
1
3
5
1.
2.
3.
4.
5.
2
2
Tripelennamine
Triprolidine
Cyclizine
Chlorcyclizine
Meclizine
1.
2.
3.
4.
Myricetin
Quercetin
Apigenin
Kaempferol
1
4
3
0
5
10
15
20
25 Min.
Column:
Alltima™ C18, 5µm, 250 x 4.6mm
Mobile Phase: A: 50mM KH2PO4, pH 3.2 B: CH3CN
Gradient:
Time: 0
5
20
%B:
25
25
70
Flowrate:
1.3mL/min
Detector:
UV at 220nm
© Copyright 2001 Alltech Associates, Inc.
2
0
1
Column:
Mobile Phase:
Flowrate:
Detector:
2
3
4
5 Min.
Alltima™ C18 Rocket™, 3µm, 53 x 7mm
0.1% TFA in H2O, pH 0.95:0.1% TFA in CH3CN(65:35)
2.5mL/min
ELSD
Contact your Alltech office or distributor for current or local prices.
Versatile Packings for a Wide Range of Applications
Taxol® and Related Taxanes in Yew
(taxus brevifolia)
1. 10-deacetylbaccatin III
2. Baccatin III
3. 7-xylosyl-10-deacetyltaxol B
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
1
2
7
4,5 6
3
0
2
1
3
Column:
Mobile Phase:
Flowrate:
Detector:
8 91011
4
13
12
5
6
7
7809
Taxinine M
7-xylosyl-10-deacetyltaxol
7-xylosyl-10-deacetyltaxol C
10-deacetyltaxol
7-xylosyltaxol
Cephalomannine (Taxol® B)
7-epi-10-deacetyltaxol
Taxol®
Taxol® C
7-epitaxol
0
8 Min.
™
Tween 80
9221
™
Alltima Phenyl Rocket , 3µm, 53x 7mm
0.05% TFA in H2O:MeOH:CH3CN (50:19:31)
2.5mL/min
UV at 230nm
Acids, Bases, and Neutrals
5
10
Column:
Mobile Phase:
Flowrate:
Detector:
15
20
25
30
™
Alltima C18, 5µm, 250 x 4.6mm
H2O:MeOH (40:60)
1.0mL/min
ELSD
5
8760
Anticonvulsants
7233
2
4
5
1
6
1. Uracil
2. Pyridine
3. Phenol
4. N,N-Dimethylaniline
5. 4-Butylbenzoic Acid
6. Toluene
1 23
4
6
3
0
0
1
Column:
Mobile Phase:
Flowrate:
Detector:
1
Aromatic Acids
4
8
Column:
Mobile Phase:
Gradient:
2
3
4
5 Min.
Alltima™ C18, 3µm, 100 x 4.6mm
50mM KH2PO4, pH3.0:CH3CN (40:60)
1.0mL/min
UV at 254nm
3
Flowrate:
Detector:
12
16
4
Alprazolam and Triazolam by the
USP XXII Method
7373
7368
1. Triazolam
2. Alprazolam
1
2
5
0
0 1 2 3 4 5 6 7 8 9 10 11 Min.
Column:
Mobile Phase:
Flowrate:
Detector:
20 Min.
Alltima™ Phenyl, 5µm, 250 x 4.6mm
A: H2O B: CH3CN
Time:
0
20
25
30
%B:
15
60
60
15
1.5mL/min
UV at 254nm
1. DL-4-Hydroxy-3methoxy-mandelic Acid
2. Homovanillic Acid
3. Vanillic Acid
4. Salicylic Acid
5. Benzoic Acid
2
1. Ethotoin
2. Phenobarbital
3. Methsuximide
4. Mephenytoin
5. Carbamazepine
6. Diazepam
Alltima™ C18, 5µm, 150 x 4.6mm
50mM KH2PO4, pH 3.2:MeOH (60:40)
1.0mL/min
UV at 254nm
Contact your Alltech office or distributor for current or local prices.
2
4
6
8
10
12
14
16
18
20 Min.
Column:
Alltima™ Silica, 5µm, 300 x 4.6mm
Mobile Phase: CH3CN:CHCl3:i-BuOH:H2O:Glacial HOAc
(85:8:5:2:0.05)
Flowrate:
2.0mL/min
Detector:
UV at 254nm
© Copyright 2001 Alltech Associates, Inc.
3
Acid and Base Deactivated for Symmetrical Peaks
Alltima™ manufacturing processes eliminate activated silanols that cause peak tailing on silica-based columns. Alltima™
columns and simple mobile phases produce sharp, symmetrical peaks with acidic compounds, basic compounds and
chelators.
Metal Chelators
Fatty Acids
8584
1
1. 2,2’-Dipyridyl
2. 8-Hydroxyquinoline
1
1.
2.
3.
4.
5.
2
8060
Capric Acid (C10)
Lauric Acid (C12)
Myristic Acid (C14)
Palmitic Acid (C16)
Stearic Acid (C18)
3
2
4
5
0
2
4
Column:
Mobile Phase:
Flowrate:
Detector:
Alltima™ C18, 5µm, 150 x 4.6mm
25mM K2HPO4, pH7.0:THF:MeOH (50:25:25)
1.0mL/min
UV at 254nm
0
6 Min.
Tricyclic Amines
4
Column:
Mobile Phase:
Flowrate:
Detector:
6
8
10
12 Min.
Alltima™ C18, 5µm, 150 x 4.6mm
THF:CH3CN:0.1% H3PO4 (25:55:20)
1.5mL/min
UV at 220nm
OPA Derivatized Amino Acids
7366
1. Fluphenazine HCl
2. Chlorpromazine HCl
3. Phenothiazine
1 2
2
1.
2.
3.
4.
5.
6.
7.
1
11 13
9
2
8
5
10
6
3
4
3
0
5
12
14
Asp
Glu
His
Ser
Arg
Gly
Thr
15
7
10
15
7596
8.
9.
10.
11.
12.
13.
14.
15.
Ala
Tyr
Met
Val
Phe
Ileu
Leu
Lys
20 Min.
™
0
1
2
3
Column:
Mobile Phase:
Flowrate:
Detector:
4
5
6
7 Min.
Alltima™ Cyano, 5µm, 250 x 4.6mm
50mM NaH2PO4, pH3.0: CH3CN (50:50)
1.5mL/min
UV at 254nm
© Copyright 2001 Alltech Associates, Inc.
4
Column:
Alltima C8, 5µm, 100 x 4.6mm
Mobile Phase: A: 50mM NaOAc, pH5.7:THF (95:5)
B: MeOH
Gradient:
Time: 0
15
%B:
10
65
Flowrate:
2.0mL/min
Detector:
Fluorescence Ex: 305-395nm Em: 430-470nm
Contact your Alltech office or distributor for current or local prices.
Stable Bonded Phases for Long Column Life
Figure 7 - An Alltima™ C18 column was washed for 500 hours at 60°C with an acetonitrile and trifluoroacetic acid mobile phase.
The separation of peptides showed no change in capacity, selectivity, or efficiency.
Alltima™ Acid Stability
7553
2
1
3
Alltima™ C18, 5µm
150 x 4.6mm
Mobile Phase: A: 0.1% TFA in
H2O:CH3CN (95:5),
B: 0.1% TFA in
H2O:CH3CN (25:75)
Gradient:
Time: 0 60
%B:
5 30
Flowrate:
1.0mL/min
UV at 220nm
5 10 15 20 25 30 35 40 45 50 55 Min. Detector:
0
Column:
45
0
7554
1. Gly-Tyr
2. Val-Tyr-Val
3. Met-Enkephalin
4. Leu-Enkephalin
5. Angiotensin II
Initial Separation
After 500 Hours of Washing
at 60°C
2
1
3
45
5 10 15 20 25 30 35 40 45 50 55 Min.
Figure 8 - Alltima™ columns are packed by proven methods and don’t void or collapse when subjected to constant high pressure. This
chromatography remains unchanged after 1,200 column volumes of a Methanol:Water (50:50) mobile phase pumped through the
column at 2.0mL/min (backpressure 3,500psig).
Alltima™ Withstands Continuous High Backpressure
8589
1. Uracil
2. Pyridine
3. Phenol
4. N,N-Dimethylaniline
5. 4-Butylbenzoic Acid
6. Toluene
Initial Separation
1
5
4
3
2
6
0
4
2
6
8
8590
After 1200 Column Volumes
of 50:50 MeOH:H2O at 2mL/min
1
5
2
Alltima™ C18, 5µm,
150 x 4.6mm
Mobile Phase: 50mM KH2PO4, pH3.0:
CH3CN (40:60)
Flowrate:
1.0mL/min
UV at 254nm
10 Min. Detector:
0
4
3
Column:
6
4
2
6
8
10 Min.
Figure 9 - Drastic solvent polarity changes don’t affect Alltima™ Columns. After 58 cycles (29 hours) of
Water ➝ Ethanol ➝ Hexane, chromatography remains unchanged.
➝
➝
Alltima™ is Unaffected by Drastic Mobile Phase Polarity Changes
Initial Separation
2
4
3
1
1
Column:
0
➝
4
3
After 58 Cycles of
H2O ➝ EtOH ➝ Hexane
1. Uracil
2. Phenol
3. N,N-Diethyl-m-toluamide
4. Toluene
2
8588
➝
8587
2
4
6
8
10 Min.
™
Alltima C18, 5µm,
150 x 4.6mm
Mobile Phase: 50mM KH2PO4, pH3.0:
CH3CN (40:60)
Flowrate:
1.0mL/min
Detector:
UV at 254nm
0
Contact your Alltech office or distributor for current or local prices.
2
4
6
8
10 Min.
© Copyright 2001 Alltech Associates, Inc.
5
A Large Selection of Hardware Formats
Alltima™ Phases are Extremely Well Suited for Applications Using MS Detection
• Stable in Popular LC/MS Mobile Phases
• No Bonded Phase Bleed
• Analyze Acids, Bases, and Neutrals with Simple, Volatile Mobile Phases
• Available in New Expedite™ Hardware for Fast, Low-Volume Applications
Expedite™ hardware is available in 10 or 20mm
lengths, and 2.1 or 4.6mm ID
New!
New!
Steroids
1
9432
2
Actual Size
1. Prednisone
2. Testosterone
3. Cortisone
4. Progesterone
4
20mm
3
0
10mm
Expedite your LC/MS analyses with Alltech’s high-speed, low-volume
Expedite™ hardware. These fast analysis columns packed with high
resolution Alltima™ media increase laboratory efficency by doubling or even
tripling sample throughput.
1
Column:
Mobile Phase:
Flowrate:
Detector:
2 Min.
Alltima™ Phenyl, 3µm, 50 x 2.1mm
45% CH3CN:50mM Ammonium Acetate, pH5.4
0.4mL/min
UV at 254nm
Alltima™ LC/MS Columns
PACKING
C18
Expedite™
Expedite™
Expedite™
Expedite™
Phenyl
Expedite™
Expedite™
Expedite™
Expedite™
Silica
Expedite™
Expedite™
Expedite™
Expedite™
Barbiturates
PARTICLE
SIZE
LENGTH X ID
PART NO.
3µm
3µm
3µm
3µm
3µm
3µm
3µm
3µm
3µm
3µm
3µm
10 x 2.1mm
20 x 2.1mm
50 x 2.1mm
100 x 2.1mm
150 x 2.1mm
10 x 4.6mm
20 x 4.6mm
100 x 1.0mm
150 x 1.0mm
33 x 7mm
53 x 7mm
43836
43803
43849
43821
43852
43837
43851
43853
43864
50603
50605
3µm
3µm
3µm
3µm
3µm
3µm
3µm
3µm
3µm
3µm
10 x 2.1mm
20 x 2.1mm
50 x 2.1mm
100 x 2.1mm
150 x 2.1mm
10 x 4.6mm
20 x 4.6mm
100 x 1.0mm
150 x 1.0mm
53 x 7mm
43809
43865
43835
43866
43813
43883
43822
43892
43854
43867
3µm
3µm
3µm
3µm
3µm
3µm
3µm
3µm
3µm
3µm
10 x 2.1mm
20 x 2.1mm
100 x 2.1mm
150 x 2.1mm
50 x 2.1mm
10 x 4.6mm
20 x 4.6mm
100 x 1.0mm
150 x 1.0mm
53 x 7mm
43891
43801
43823
43884
43876
43889
43886
43824
43855
43877
© Copyright 2001 Alltech Associates, Inc.
6
9433
5
PRICE
2
1. Barbital
2. Phenobarbital
3. Talbital
4. Amobarbital
5. Mephobarbital
1
3
4
0
1
Column:
Mobile Phase:
Flowrate:
Detector:
2 Min.
Alltima™ C18, 3µm, 50 x 2.1mm
40% CH3CN:50mM Ammonium Acetate, pH5.4
0.5mL/min
UV at 230nm
Specifications for Alltima™ Packing Materials
Alltima™ Packing Specifications
C18
C18-WP
C18-LL
AQ New!
C8
C4-WP
Phenyl
Cyano
Amino
Silica
PARTICLE SIZE
PORE SIZE
CARBON LOAD
ENDCAPPED
3, 5, 10µm
5µm
5µm
3, 5µm
3, 5, 10µm
5µm
3, 5µm
3, 5µm
3, 5µm
3, 5, 10µm
100Å
200Å
100Å
100Å
100Å
200Å
100Å
100Å
100Å
100Å
16%
17%
9%
15%
9%
6%
7.5%
—
—
—
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
No
—
Contact your Alltech office or distributor for current or local prices.
A Large Selection of Hardware Formats
Alltima™ Analytical Columns
Alltima™ Cartridge Columns*
LENGTH X ID
INDUSTRY
STANDARD
PART NO.
WATERS
INSTRUMENT
PART NO.
50 x 4.6mm
100 x 4.6mm
150 x 4.6mm
150 x 4.6mm
250 x 4.6mm
150 x 4.6mm
250 x 4.6mm
81412
81382
81387
88052
88056
88302
88307
—
81383
81388
88053
88057
88303
88308
5µm
5µm
3µm
3µm
150 x 4.6mm
250 x 4.6mm
100 x 4.6mm
150 x 4.6mm
88925
88930
88935
88940
88945
88950
88955
88960
C18-WP
5µm
5µm
150 x 4.6mm
250 x 4.6mm
81194
81196
81195
81197
C18-LL
5µm
5µm
150 x 4.6mm
250 x 4.6mm
88069
88099
88082
88235
3µm
3µm
3µm
5µm
5µm
10µm
10µm
50 x 4.6mm
100 x 4.6mm
150 x 4.6mm
150 x 4.6mm
250 x 4.6mm
150 x 4.6mm
250 x 4.6mm
81413
81392
81397
88072
88076
88312
88317
—
81393
81398
88073
88077
88313
88318
5µm
5µm
150 x 4.6mm
250 x 4.6mm
81198
81202
81199
81204
Phenyl
3µm
3µm
3µm
5µm
5µm
50 x 4.6mm
100 x 4.6mm
150 x 4.6mm
150 x 4.6mm
250 x 4.6mm
81178
81181
81188
88087
88092
—
81184
81191
88088
88093
Cyano
3µm
3µm
3µm
5µm
5µm
50 x 4.6mm
100 x 4.6mm
150 x 4.6mm
150 x 4.6mm
250 x 4.6mm
81179
81182
81189
88180
88189
—
81186
81192
88182
88191
3µm
3µm
3µm
5µm
5µm
50 x 4.6mm
100 x 4.6mm
150 x 4.6mm
150 x 4.6mm
250 x 4.6mm
81180
81183
81190
88205
88217
—
81187
81193
88207
88218
3µm
3µm
3µm
5µm
5µm
10µm
10µm
50 x 4.6mm
100 x 4.6mm
150 x 4.6mm
150 x 4.6mm
250 x 4.6mm
150 x 4.6mm
250 x 4.6mm
81414
81404
81409
88123
88171
88322
88327
—
81405
81410
88124
88172
88323
88328
PACKING
C18
AQ New!
PARTICLE
SIZE
3µm
3µm
3µm
5µm
5µm
10µm
10µm
PRICE
PARTICLE
SIZE
LENGTH X ID
INDUSTRY
STANDARD
PART NO.
C18
5µm
5µm
150 x 4.6mm
250 x 4.6mm
88061
88062
Phenyl
5µm
5µm
150 x 4.6mm
250 x 4.6mm
88095
88096
Silica
5µm
5µm
150 x 4.6mm
250 x 4.6mm
88125
88173
PACKING
Endfitting, SS, 1 set
PRICE
28197
*Requires endfitting set (Part No. 28197).
Alltima™ Prep Columns New!
PARTICLE
SIZE
LENGTH X ID
INDUSTRY
STANDARD
PART NO.
C18
5µm
5µm
5µm
5µm
10µm
10µm
10µm
10µm
150 x 10mm
250 x 10mm
150 x 22mm
250 x 22mm
150 x 10mm
250 x 10mm
150 x 22mm
250 x 22mm
81102
88063
81106
81105
81107
88334
81108
88335
C8
5µm
5µm
5µm
5µm
10µm
10µm
10µm
10µm
150 x 10mm
250 x 10mm
150 x 22mm
250 x 22mm
150 x 10mm
250 x 10mm
150 x 22mm
250 x 22mm
81109
88081
81112
81110
81114
88336
81115
88337
Silica
5µm
5µm
5µm
5µm
10µm
10µm
10µm
10µm
150 x 10mm
250 x 10mm
150 x 22mm
250 x 22mm
150 x 10mm
250 x 10mm
150 x 22mm
250 x 22mm
81117
81116
81119
81118
81121
88338
81125
88339
PARTICLE
SIZE
LENGTH X ID
PART NO.
3µm
3µm
33 x 7mm
53 x 7mm
50603
50605
3µm
53 x 7mm
50765
3µm
3µm
33 x 7mm
53 x 7mm
50607
50609
PACKING
C8
C4-WP
Amino
Silica
Alltima™ Rocket™ Columns
PACKING
C18
AQ
C8
Contact your Alltech office or distributor for current or local prices.
PRICE
New!
PRICE
© Copyright 2001 Alltech Associates, Inc.
7
Alltima™ Guard Columns and Validation Kits
Protect Your Alltima™ Columns with
Alltima™ Guard Columns
Alltima™ C18 Validation Kits Simplify
Method Development
• Simple – Fewer
Parts, Fewer Seals,
Fewer Problems
• Fast – Change
Cartridges in
Seconds without
Tools
• Efficient – Matched
Media and Low Dead
Volume Maintain Chromatographic Performance
When validating a method, Alltima™ is the column of choice
because of its excellent batch to batch, and column to
column reproducibility. The Alltima™ C18 Method
Validation Kit helps test method ruggedness prior to routine
use. This kit contains three columns made from three
different batches of either 3µm or 5µm Alltima™ C18
packing.
1656
DESCRIPTION
Alltima™ All-Guard™ Guard Columns
DESCRIPTION
PARTICLE SIZE
LENGTH X ID
QTY
All-Guard™ Cartridges (Requires Guard Holder)
C18
5µm
7.5 x 4.6mm
3
C18-WP
5µm
7.5 x 4.6mm
3
C18-LL
5µm
7.5 x 4.6mm
3
AQ
5µm
7.5 x 4.6mm
3
C8
5µm
7.5 x 4.6mm
3
C4-WP
5µm
7.5 x 4.6mm
3
Phenyl
5µm
7.5 x 4.6mm
3
Cyano
5µm
7.5 x 4.6mm
3
Amino
5µm
7.5 x 4.6mm
3
Silica
5µm
7.5 x 4.6mm
3
PART NO.
Alltima™ C18 Validation Kits
PRICE
96080
96412
96285
96344
96081
96413
96082
96084
96085
96083
PART NO.
Alltima™ C18 Method Validation Kits
100 x 4.6mm, 3µm Kit
(3 Columns from 3 Different Batches)
150 x 4.6mm, 5µm Kit
(3 Columns from 3 Different Batches)
250 x 4.6mm, 5µm Kit
(3 Columns from 3 Different Batches)
PRICE
11350
11541
11543
Simplify Your HPLC Connections
with Fittings and Accessories
from Alltech
Call to request brochure #441.
Guard Holder (Includes Direct-Connect Column Coupler)
All-Guard™ Cartridge Holder
Ea
80101
Your Online Technical Resource for Chromatography
The trademarks referred to herein are the property of their respective owners.
Contact your Alltech office or distributor for current or local prices.
Alltech Corporate Headquarters:
2051 Waukegan Road • Deerfield, IL 60015
Phone: 847-948-8600 • Fax: 847-948-0477
Email: [email protected]
Web Site: http://www.alltechWEB.com
Your Local Distributor is:
08/01 #458
© Copyright 2001 Alltech Associates, Inc.
Alltech reserves the right to change prices and/or specifications without prior notification.
Use of Our Products: Alltech Associates, Inc., supplies chromatographic equipment reagents and accessories
for non-medical laboratory use only. This means our products: ... may NOT be used as drugs, ...may NOT be
used as cosmetics, ...may NOT be used as medical devices or as in vitro diagnostic devices.
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REGISTERED COMPANY
QUALITY
MANAGEMENT
015
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