研 究
業 績
学位論文
木村
聡「Biogenesis and function of cellulose in the Tunicates」博士（農学）京都大学, (1998)
原著論文
1)
Mizuta, S., Watanabe, A., Kimura, S., Yoshida, K.
Possible involvement of membrane fluidity in helicoidal microfibrillar orientation in the coenocytic green
algae, Boergesenia forbesii. Protoplasma 180: 82-91 (1994)
2)
Mizuta, S., Kaneko, M., Kimura, S., Okuda, K.
Experimental studies on the stability of the cortical microtubule cytoskeleton in relation to polarity and
cell elongation in the coenocytic green alga, Chaetomorpha moniligera. Annals of Botany (London) 73:
273-280 (1994)
3)
Kimura, S., Mizuta, S.
Role of the microtubule cytoskeleton in alternating changes in cellulose microfibril orientation in the
coenocytic green alga, Chaetomorpha moniligera. Planta (Heidelberg) 193: 21-31 (1994)
4)
Kimura, S., Mizuta, S.
Cell wall expansion in the marine coenocytic green algae, Chaetomorpha and Boergesenia.
Botanica Marina 38: 21-30 (1995)
5)
Kimura, S., Itoh, T.
Evidence for the role of the glomerulocyte in cellulose synthesis in the tunicate, Metandrocarpa uedai.
Protoplasma 186: 24-33 (1995)
6)
Kimura, S., Itoh, T.
New cellulose synthesizing complexes (= terminal complexes) involved in animal cellulose biosynthesis
in the tunicate, Metandrocarpa uedai. Protoplasma 194: 151-163 (1996)
7)
Kimura, S., Itoh, T.
Cellulose network of hemocoel in selected compound styelid ascidians.
Journal of Electron Microscopy 46(4): 327-335 (1997)
8)
Hirose, E., Kimura, S., Itoh, T., Nishikawa, Jun.
Tunic morphology and cellulosic components of pyrosomas, doliolids, and salps (Thaliacea,
Urochordata). Biological Bulltain 196: 113-120 (1998)
9)
Kimura, S., Itoh, T.
A new cellulosic structure, the tunic cord in the ascidian, Polyandrocarpa misakiensis.
Protoplasma 204: 94-102 (1998)
10) Helbert, W., Sugiyama, J., Kimura, S., Itoh, T.
High-resolution electron microscopy on ultrathin sections of cellulose microfibrils generated by
glomerulocytes in Polyzoa vesiculiphora. Protoplasma 203: 84-90 (1998)
11) Kimura, S., Laosinchai, W., Itoh, T., Cui, X., Linder, C. R., Brown, R. M. Jr.
Immunogold labeling of rosette terminal cellulose synthesizing complexes in the vascular plant Vigna
angularis. Plant Cell 11: 2075-2085 (1999)
12) Kimura, S., Sakurai, N., Itoh, T.
Different distribution of cellulose synthesizing complexes in brittle and non-brittle strains of barley.
Plant and Cell Physiology 40(3): 335-338 (1999)
13) Matsuoka, T., Tokumori, D., Kotsuki, H., Ishida, M., Matsushita, M., Kimura, S., Itoh, T., Checcucci, G.
Analysis of surface of photoreceptor organelle and blepharismin-associated protein in unicellular
eukaryote Blepharisma. Photochemistry and Photobiology 72(5): 709-713 (2000)
14) Kimura, S., Chen. H. P., Saxena, I. M., Brown, R. M. Jr., Itoh, T.
Localization of c-di-GMP -binding protein wiyh the linear terminal complexes of Acetobacter xylinum.
Journal of Bacteriology 183: 5668-5674 (2001)
15) Kimura, S., Ohshima, C., Hirose, E., Nishikawa, J., Itoh, T.
Cellulose in the House of the Appendicularian, Oikopleura rufescens. Protoplasma 216: 71-74 (2001)
16) Carson, D.T., Kimura, S., Fujino, T., Itoh, T., Ashworth, E. N.
Elimination of artifactual immunogold labeling from secondary walls of woody stem sections.
Plant Cell Reports 20: 786-790 (2002)
17) Carson, D.T., Fujino, T., Kimura, S., Baba, K., Itoh, T., Ashworth, E. N.
Novel plasmodesmata association of dehydrin-like proteins in cold-acclimated red-osier dogwood
(Cornus sericea). Tree Physiology 23: 759-768 (2003)
18) Itoh, T., Kimura, S., Brown, R. M. Jr.
Theoretical considerations of immunogold labeling of cellulose synthesizing terminal complexes.
Cellulose 11: 385-394 (2004)
19) Kimura, S., Itoh T.
Cellulose synthesizing terminal complexes in the ascidians. Cellulose 11: 377-383 (2004)
20) Kimura, S., Kameyama, A., Nakaya, S., Ito, H., Narimatsu, H.
Direct on-membrane glycoproteomic approach using MALDI-TOF mass spectrometry coupled with
microdispensing of multiple emzymes. Journal of Proteome Research 6: 2488-2494. (2007)
21) Saito, T., Kimura, S., Nishiyama, Y., Isogai, A.
Cellulose nanofibers prepared by TEMPO-mediated oxidation of native cellulose.
１
Biomacromolecules 8: 2485-2491. (2007)
22) Tomoko Seyama, Satoshi Kimura, Hamako Sasamoto, Hisashi Abe, Tetsuo Kondo.
Spinning of a gigantic bundle of hollow fibrils by a spirally moving higher plant protoplast.
Planta 227: 1187-1197 (2008)
23) Jie Cai, Satoshi Kimura, Masahisa Wada, Shigenori Kuga, Lina Zhang.
Cellulose aerogels from aqueous alkali hydroxide-urea solution. ChemSusChem 1: 149-154 (2008)
24) J. Cai, S. Kimura, M. Wada, and S. Kuga.
Nanoporous cellulose as metal nanoparticles support. Biomacromolecules, 10(1), 87-94 (2009)
25) Tsuguyuki Saito, Masayuki Hirota, Naoyuki Tamura, Satoshi Kimura, Hayaka Fukuzumi, Laurent Heux,
Akira Isogai.
Individualization of nano-sized plant cellulose fibrils by direct surface carboxylation using TEMPO
catalyst under neutral conditions. Biomacromolecules 10: 1992-1996 (2009)
26) M. Hiraishi, K. Igarashi, S. Kimura, M. Wada, M. Kitaoka, M. Samejima.
Synthesis of highly ordered cellulose II in vitro using cellodextrin phosphorylase.
Carbohydrate Research, 344(18), 2468-2473 (2009)
27) K. Igarashi, A. Koivula, M. Wada, S. Kimura, M. Penttilä, M. Samejima.
High-speed atomic force microscopy visualizes processive movement of Trichoderma reesei
cellobihydrolase I on crystalline cellulose.
Journal of Biological Chemistry, 284(52), 36186-36190 (2009)
28) Hiroshi Niimura, Tomoya Yokoyama, Satoshi Kimura, Yuji Matsumoto, Shigenori Kuga.
AFM observation of ultrathin microfibrils in fruit tissues. Cellulose 17: 13-18 (2010)
29) K. Kobayashi, S. Kimura, E. Togawa, M. Wada.
Crystal transition between hydrate and anhydrous β-chitin monitored by synchrotron X-ray fiber
diffraction. Carbohydrate Polymers, 79(4), 882-889 (2010)
30) K. Kobayashi, S. Kimura, E. Togawa, M. Wada, S. Kuga.
Crystal transition of paramylon with dehydration and hydration.
Carbohydrate Polymers, 80(2), 492-498 (2010)
31) Y. Ogawa, S. Kimura, M. Wada, S. Kuga
Crystal analysis and high-resolution imaging of microfibrillar α-chitin from Phaeocystis.
Journal of Structural Biology 171: 111-116 (2010)
32) K. Kobayashi, S. Kimura, E. Togawa, M. Wada
Crystal transition from Na-cellulose IV to cellulose II monitored using synchrotron X-ray diffraction.
Carbohydrate Polymers, 83(2), 483-488, (2011)
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33) S. Kimura, N. Isobe, M. Wada, S. Kuga, J. H. Ko, U. J. Kim
Enzymatic hydrolysis of chitosan-dialdehyde cellulose hydrogels.
Carbohydrate Polymers, 83(4), 1850-1853, (2011)
34) N. Isobe, M. Sekine, S. Kimura, M. Wada, S. Kuga
Anomalous reinforcing effects in cellulose gel-based polymeric nanocomposites.
Cellulose, 18(2), 327-333, (2011)
35) N. Isobe, U. J. Kim, S. Kimura, M. Wada, S. Kuga
Internal surface polarity of regenerated cellulose gel depends on the species used as coagulant.
Journal of Colloid and Interface Science, 359(1), 194-201, (2011)
36) N. Isobe, D. S. Lee, Y. J. Kwon, S. Kimura, S. Kuga, M. Wada, U. J. Kim
Immobilization of protein on cellulose hydrogel. Cellulose, 18(5), 1251-1256, (2011)
37) K. Kobayashi, S. Kimura, E. Togawa, M. Wada
Crystal transition from cellulose II hydrate to cellulose II.
Carbohydrate Polymers, 86(2), 975-981, (2011)
38) X. Su, S. Kimura, M. Wada, S. Kuga
Stoichiometry and stability of cellulose-hydrazine complexes. Cellulose, 18(3), 531-537, (2011)
39) Y. Ogawa, S. Kimura, M. Wada
Electron diffraction and high-resolution imaging on highly-crystalline β-chitin microfibril
Journal of Structural Biology, 176(1), 83-90, (2011)
40) K. Igarashi, T. Uchihashi, A. Koivula, M. Wada, S. Kimura, T. Okamoto, M. Penttilä, T. Ando, M.
Samejima
Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface.
Science, 333(6047), 1279-1282, (2011)
41) D. Sawada, Y. Nishiyama, P. Langan, T. Forsyth, S. Kimura, M. Wada
Direct determination of the hydrogen bonding arrangement in anhydrous β-chitin by neutron fiber
diffraction. Biomacromolecules, 13(1), 288-291, (2012)
42) J. Cai, S. Liu, J. Feng, S. Kimura, M. Wada, S. Kuga, L. Zhang
Cellulose–silica nanocomposite aerogels by in situ formation of silica in cellulose gel
Angewandte Chemie International Edition, 51(9), 2076-2079, (2012)
43) N. Isobe, S. Kimura, M. Wada, S. Kuga
Mechanism of cellulose gelation from aqueous alkali-urea solution
Carbohydrate Polymers, 89(4), 1298-1300, (2012)
44) K. Kobayashi, S. Kimura, U. J. Kim, K. Tokuyasu, M. Wada
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Enzymatic Hydrolysis of Cellulose Hydrates. Cellulose, 19, 967-974, (2012)
45) Yu Ogawa, Satoshi Kimura, Yukie Saito, Masahisa Wada
Infrared study on deuteration of highly-crystalline chitin.
Carbohydrate Polymers, 90 (1), 650-657, (2012)
46) Furukawa F, Watanabe S, Kimura S, Kaneko T.
Potassium excretion through ROMK potassium channel expressed in gill mitochondrion-rich cells of
mozambique tilapia. American Journal of Physiology, Regulatory, Integrative and Comparative
Physiology, 302, R568-R576, (2012)
47) Daisuke Sawada, Yoshiharu Nishiyama, Paul Langan, V. Trevor Forsyth, Satoshi Kimura, Masahisa
Wada.
Water in crystalline fibers of dihydrate β-chitin results in unexpected absence of intramolecular
hydrogen bonding. PLoS One, 7(6), e39376, (2012)
48) K. Igarashi, T. Uchihashi, A. Koivula, M. Wada, S. Kimura, M. Penttilä, T. Ando, M. Samejima.
Visualization of cellobiohydrolase I from Trichoderma reesei moving on crystalline cellulose using
high-speed atomic force microscopy. Methods in Enzymology, 510, 169-182, (2012)
49) K. Kobayashi, S. Kimura, E. Togawa, M. Wada
Thermal expansion behavior of hydrate paramylon in the low-temperature region.
Carbohydrate Polymers, 91, 543-548, (2013)
50) N. isobe, K. Noguchi, Y. Nishiyama, S. Kimura, M. Wada, S. Kuga
Role of urea in alkaline dissolution of cellulose. Cellulose, 20, 97-103 (2013)
51) D. Sawada, S. Kimura, Y. Nishiyama, P. Langan, M. Wada
The crystal structure of mono-ethylenediamine β-chitin from synchrotron X-ray fiber diffraction.
Carbohydrate Polymers, 92, 1737-1742, (2013)
52) S. Fujisawa, T. Saito, S. Kimura, T. Iwata, A. Isogai
Surface engineering of ultrafine cellulose nanofibrils toward polymer nanocomposite materials.
Biomacromolecules, 14, 1541-1546, (2013)
53) X. Su, S. Kimura, M. Wada, U.J. Kim, S. Kuga
Complexation of hydrazine with native cellulose in water and toluene. Cellulose, 20, 1023-1029, (2013)
54) K. Kobayashi, S. Kimura, L. Heux, M. Wada
Crystal transition between hydrate and anhydrous (1-3)-β-D-xylan from Penicillus dumetosus.
Carbohydrate Polymers, 97, 105-110, (2013)
55) N. Isobe, X. Chen, U.J. Kim, S. Kimura, M. Wada, T. Saito, A. Isogai
TEMPO-oxidized cellulose hydrogel as a high-capacity and reusable heavy metal ion adsorbent.
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Journal of Hazardous Materials, 260, 195-201, (2013)
56) Y. Ogawa, K. Kobayashi, S. Kimura, Y. Nishiyama, M. Wada, S. Kuga
X-ray texture analysis indicates downward spinning of chitin microfibrils in tubeworm tube.
Journal of Structural Biology, 184, 212-216 (2013)
57) D. Oka, K. Kobayashi, N. Isobe, Y. Ogawa, T. Yokoyama, S. Kimura, U.J. Kim, K. Tokuyasu, M. Wada
Enzymatic hydrolysis of wood with alkaline treatment. Journal of Wood Science, 59, 484-488, (2013)
58) M. Wu, D. Sawada, Y. Ogawa, S. Kimura, M. Wada, S. Kuga
Crystalline alignment of metal ions templated by β-chitin ester. Cellulose, 20, 2757-2763, (2013)
59) Y. Ogawa, H. Hidaka, S. Kimura, U.J. Kim, S. Kuga, M. Wada
Formation and stability of cellulose-copper-NaOH crystalline complex. Cellulose, 21, 999-1006, (2014)
60) D. Sawada, Y. Ogawa, S. Kimura, Y. Nishiyama, P. Langan, M. Wada
Solid-solvent molecular interactions observed in crystal structure of β-chitin complexes.
Cellulose, 21, 1007-1014, (2014)
61) N. Isobe, Y. Nishiyama, S. Kimura, M. Wada, S. Kuga
Origin of hydrophilicity of cellulose hydrogel from aqueous LiOH/urea solvent coagulated with alkyl
alcohols. Cellulose, 21, 1043-1050, (2014)
62) Y. Ogawa, K. Noda, S. Kimura, M. Kitaoka, M. Wada
Facile preparation of highly crystalline lamellae of (1→3)-β-D-glucan using an extract of Euglena gracilis.
International Journal of Biological Macromolecules, 64, 415-419, (2014)
63) Y.J. Yang, J.M. Shin, T.H. Kang, S. Kimura, M. Wada, U.J. Kim
Cellulose dissolution in aqueus lithium bromide solutions. Cellulose, 21, 1175-1181, (2014)
64) Chihaya Yamada, Souichiro Kato, Satoshi Kimura, Masaharu Ishii, Yasuo Igarashi
Reduction of Fe(III) oxides by phylogenetically and physiologically diverse thermophilic methanogens.
FEMS Microbioly Ecology, 89, 637–645, (2014)
65) Philip-Kunio Naito Yu Ogawa, Satoshi Kimura, Tadahisa Iwata, Masahisa Wada
Crystal transition from hydrated chitosan and chitosan/monocarboxylic acid complex to anhydrous
chitosan investigated by X-ray diffraction.
Journal of Polymer Science, Part B: Polymer Physics, 53, 1065–1069, (2015).
66) Kayoko Kobayashia, Satoshi Kimura, Philip-Kunio Naito, Eiji Togawa, Masahisa Wada
Thermal expansion behavior of A- and B-type amylose crystals in the low-temperature region.
Carbohydrate Polymers, 131, 399–406, (2015)
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67) Fengping Jia, Jiayan Luo, Ryosuke Takahashi, Chie Uehira, Shinichi Kitamura, Satoshi Kimura,
Ung-Jin Kim, Takao Itoh
Structure and chemical contents of mucilage cells in Pseudolarix and Abies bark.
IAWA Journal, 36 (3), 300-310 (2015)
68) Satoshi Kimura, Kei Nakayama, Masahisa Wada, Ung-Jin Kim, Kaoru Azumi, Takao Ojima, Akino
Nozawa, Shin-Ichi Kitamura, Euichi Hirose
Cellulose is not degraded in the tunic of the edible ascidian Halocynthia roretzi contracting soft tunic
syndrome. Diseases of Aquatic Organisms, 116, 143-148 (2015)
69) Bruno Frka-Petesic, Junji Sugiyama, Satoshi Kimura, Henri Chanzy, Georg Maret
Negative Diamagnetic anisotropy and birefringence of cellulose nanocrystals.
Macromolecules, 48, 8844−8857 (2015)
70) Daichi Yamashita, Satoshi Kimura, Masahisa Wada, Keiji Takabe
Improved Maule color reaction provides more detailed information on syringyl lignin distribution in
hardwood. Journal of Wood Science, 62, 131-137 (2016)
71) Daisuke Sawada, Yu Ogawa, Yoshiharu Nishiyama, Eiji Togawa, Satoshi Kimura, and Paul Langan.
Molecular interactions in an α-citin/hydrazine complex: Dynamic hydrogen bonds and improvement of
polymeric crystallinity.
Crystal Growth & Design, 16, 3345–3352 (2016)
72) Sakarin Puanglek, Satoshi Kimura, Yukiko Enomoto-Rogers, Taizo Kabe, Makoto Yoshida, Masahisa
Wada and Tadahisa Iwata
In vitro synthesis of linear α-1,3-glucan and chemical modification to ester derivatives exhibiting
outstanding thermal properties.
Scientific Reports, 6, Article number 30479 (2016)
73) Yukiko Enomoto-Rogers, Satoshi Kimura, and Tadahisa Iwata
Soft, tough, and flexible curdlan hydrogels and organogels.
Polymer, 100, 143-148 (2016)
著書
1)
Kimura, S., Itoh T.
Occurrence of high crystalline cellulose in the most primitive tunicate, Appendicularian.
In Molecular breeding of woody plants (Morohoshi and Komamine, eds.),
Elsevier Science B.V., pp. 121-125 (2001)
2)
伊東隆夫, 木村 聡.
６
Chapter 3-6: 細胞壁
In 新板 植物の細胞を観る実験プロトコール (福田, 西村, 中野, eds.), 秀潤社, pp. 185-196 (2006)
3)
Kimura, S, Itoh, T.
Chapter 13: Biogenesis and function of cellulose in the tunicates
In Cellulose: Molecular and Structural Biology (Brown and Saxena, eds.), Springer, pp. 217-236. (2007)
4)
Itoh, T, Kimura, S, Brown RM Jr.
Chapter 14: Immunogold labeling of cellulose-synthesizing terminal complexes
In Cellulose: Molecular and Structural Biology (Brown and Saxena, eds.), Springer, pp. 237-256. (2007)
5)
今井友也,木村 聡,川越 靖,杉山淳司
Chapter 2.3: セルロースの構造と生合成の接点
Chapter 2.4: セルロース生合成の生化学
Chapter 2.5: セルロース生合成の分子機能
In 木質の形成 第二版（福島, 船田, 杉山, 高部, 山本, eds）, 海青社, pp. 180-228 (2011)
6)
木村 聡
バクテリアセルロースの培養と精製
In セルロースのおもしろ科学とびっくり活用（セルロース学会, eds.）, 講談社, pp. 46-47 (2012)
7)
木村 聡, 伊東隆夫
Chapter 7.1 イメージング
In 植物細胞壁（西谷, 梅澤 eds.）
講談社, pp. 260-264 (2013)
8)
木村 聡
Chapter 4.5.2 セルロース合成酵素の可視化
In 植物細胞壁実験法（石井, 石水, 梅澤, 加藤, 岸本, 小西, 松永 eds.）
弘前大学出版会, pp. 195-199 (2016)
総説
1)
木村 聡, 伊東隆夫
ホヤで見られる新しいセルロース合成の場. 電子顕微鏡 31(1): 59-61 (1996)
2)
伊東隆夫, 木村 聡
動物（ホヤ）で初めて観察されたセルロース合成酵素複合体. 化学と生物 35(6): 400-402 (1997)
3)
木村 聡, 伊東隆夫
ホヤセルロースの構造と機能. Cellulose Communications 7(3): 117-120 (2000)
4)
Itoh, T., Kimura, S.
７
Immunogold labeling of terminal cellulose-synthesizing complexes.
Journal of Plant Research 114: 483-489 (2001)
5)
Kimura, S., Kondo, T.
Recent progress in cellulose biosynthesis. Journal of Plant Research 115: 297-302 (2002)
6)
木村 聡
セルロース生合成研究の最近の進歩. APAST No. 45: 15-19 (2002)
7)
木村 聡
凍結割断法により植物細胞壁の形態形成を探る. 顕微鏡 43: 35-39 (2008)
8)
木村 聡, 中島啓介
セルロース生合成研究の最近の進歩. Cellulose Communications 15: 109-113 (2008)
9)
Satoshi Kimura, Yu Ogawa
Preparation of tunicate cellulose. Cellulose Communications, 19(1), 40-42, (2012)
特許
1)
Method for cleavage of sugar chain from glycoprotein, mass spectrometry for sugar chain, and mass
spectrometry for glucoprotein. Pub. No.: WO/2006/109858
Inventors: Satoshi Kimura, Akihiko Kameyama, Hisashi Narimatsu
Applicants: Shimadu Corporation, National Institute of Advanced Industrial Science Technology
2)
精製セルロース球状粒子の製造方法
出願 2011-282903 (2011/12/26) 公開 2013-133355 (2013/07/08)
発明者：空閑重則、和田昌久、木村聡、岡本敏. 出願人：国立大学法人東京大学
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