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Nucleotide Solutions
Product Listing
Product Overview
New England Biolabs supplies a selection of highly pure nucleotides for use with DNA and RNA polymerases.
Deoxynucleotide Solution Set (NEB #N0446)
The Deoxynucleotide Solution Set contains four separate 100 mM solutions of ultrapure nucleotides (dATP, dCTP, dGTP, and dTTP).
Deoxynucleotide Solution Mix (NEB #N0447)
The Deoxynucleotide Solution Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP. Each nucleotide is present at a
concentration of 10 mM in the mixture for a total dNTP concentration of 40 mM.
7-deaza-dGTP* (NEB #N0445)
7-deaza-dGTP is a useful additive for the PCR of GC-rich templates; contains a 5 mM solution of 7-deaza-dGTP as a dilithium salt.
* licensed from Roche Diagnostics GmbH
Acyclonucleotide Set (NEB #N0460)
The Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP), supplied as a dry powder.
dATP Solution (NEB #N0440)
The dATP solution contains 0.25 ml of 100 mM ultrapure dATP.
Ribonucleotide Solution Set (NEB #N0450)
Ribonucleotide Solution Set consists of four separate 100 mM solutions of ATP, GTP, CTP and UTP.
Ribonucleotide Solution Mix (NEB #N0466)
The Ribonucleotide Solution Mix is an equimolar mixture of ribonucleotide triphosphates (rATP, rCTP, rGTP and rUTP). Each is supplied at
a concentration of 80 mM for a total concentration of 320 mM.
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FAQs
Protocols
DNA Polymerase
Selection Chart
PCR Selection Tool
What is 7-deaza-dGTP used for?
What is supplied with the Deoxynucleotide Solution Mix?
How does this product differ from the Deoxynucleotide Solutions Set (NEB# N0446)?
What is supplied with the Deoxynucleotide Solution Set?
How much is in each vial of the Deoxynucleotide Solution Set?
What is in the Deoxynucleotide Solution Mix?
Polymerases and Amplification FAQs
Loop-mediated Isothermal Amplification (LAMP)
DNA Polymerase Selection Chart
NEB offers a guidelines for choosing the correct DNA polymerase for your application by providing a list of specific
properites.
Several factors govern which polymerase should be used in a given application, including:
Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the
template?
Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain
as part of the protocol? If so, will they be removed from the 5´ or the 3´ end?
Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation
desirable?
Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized
products?
PCR Selection Tool
Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme
technology and bring unparalleled confidence to your experiments.