Characterization of Canadian grade standards and lean yield prediction for cows A. Rodas-González, M. Juárez, W. M. Robertson, I. L. Larsen, and J. L. Aalhus1 Can. J. Anim. Sci. Downloaded from pubs.aic.ca by Alberta Government Library on 03/08/13 For personal use only. Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C & E Trail, Lacombe, Alberta, Canada T4L 1W1. Received 8 August 2012, accepted 21 November 2012. Rodas-González, A., Juárez, M., Robertson, W. M., Larsen, I. L. and Aalhus, J. L. 2013. Characterization of Canadian grade standards and lean yield prediction for cows. Can. J. Anim. Sci. 93: 99107. Commercial carcasses (n 120) were selected to benchmark the current Canadian grading system for cows (D1, D2, D3, D4; 50% ossification) in comparison to A/AA grades youthful carcasses [over (OTM) and under (UTM) 30 mo of age based on dentition but B50% ossification]. With the exception of the D3 and D4 grades, D1 and D2 carcass grades had similar carcass yield attributes compared with OTM and UTM carcasses; however, rib-eye area from UTM carcasses was the largest (PB0.05), followed by D1, D2 and OTM. As expected, both OTM and UTM grades had lower ossification scores (PB0.05); however, D4 grade showed the highest marbling score (PB0.05). For carcass composition, compared with all other grades, the D3 grade had the highest proportion of lean (PB0.05) due to a lower proportion of dissectible fat (PB0.05); however, it had the lightest carcass weight (PB0.05). Using simple measures of carcass characteristics (grade fat, rib-eye area, marbling and ossification) a prediction equation to estimate lean yield (R2 0.825; Cp 4.31) could be used to more accurately assess carcass value in cows; however, validation of the equation on a separate population would be required before its application. Key words: Canadian grading system, carcass, cows, cutability, lean yield, predictive equation Rodas-González, A., Juárez, M., Robertson, W. M., Larsen, I. L. et Aalhus, J. L. 2013. Caractérisation des normes de classement canadiennes et prévision du rendement en viande maigre des vaches. Can. J. Anim. Sci. 93: 99107. Les auteurs ont sélectionné des carcasses commerciales (n 120) afin d’étalonner le système de classement canadien actuel pour les vaches (D1, D2, D3, D4;50 % d’ossification) et le comparer aux classes A/AA attribuées aux carcasses des animaux plus jeunes [plus (OTM) et moins (UTM) de trente mois, selon la dentition B50 % d’ossification]. Hormis les classes D3 et D4, les carcasses D1 et D2 présentaient les mêmes paramètres de rendement que les carcasses des animaux OTM et UTM. Les carcasses UTM se caractérisaient néanmoins par la plus grande superficie de faux-filet (PB0,05); venaient ensuite les carcasses D1, D2 et OTM. Comme il fallait s’y attendre, l’ossification est moins grande (PB0,05) chez les animaux OTM et UTM; cependant, les carcasses D4 avaient le meilleur persillé (PB0,05). En ce qui concerne la composition des carcasses, ce sont celles de la classe D3 qui donnent la plus forte proportion de viande maigre (PB0,05), la raison étant un pourcentage inférieur de gras séparable (PB0,05); ces carcasses étaient néanmoins aussi les plus légères (PB0,05). En recourant simplement à la quantification des paramètres de la carcasse (épaisseur moyenne du lard dorsal, surface du faux-filet, persillé et ossification), les auteurs ont formulé une équation qui prévoit le rendement estimatif en viande maigre (R2 0,825; Cp 4,31) et dont on pourrait se servir pour évaluer avec plus de précision la valeur des carcasses des vaches. Il conviendrait cependant de valider cette équation avec une population distincte avant de l’utiliser. Mots clés: Système de classement canadien, carcasse, vaches, rendement boucher, rendement en viande maigre, équation prévisionnelle In Canada, mature cows (50% ossification) are typically sold for consumption as manufacturing beef, relying heavily on the US market (Canfax 2004). Disruptions to this market from 2003 to 2007 arising from a single case of BSE in May 2003 highlighted the vulnerability of the mature animal market to US trade (Rude et al. 2007). Canadian mature cow grades (D grades) have increased significantly in the past years (from 3.88% of the total graded carcasses in 2003 to 10.09% in 2011), creating a calculated annual surplus in excess of 160 000 t of meat from mature cows (Canfax 1 Corresponding author (e-mail: Jennifer.Aalhus@agr. gc.ca). Can. J. Anim. Sci. (2013) 93: 99107 doi:10.4141/CJAS2012-091 2004, 2011), leading to a drop in price for cow grades. Hence, the beef industry has been seeking opportunities to expand domestic markets and improve the carcass value of mature cows. Current ‘‘cow’’ grades in Canada are based on a broad classification of carcass types (Canada Gazette 1992) and little published information exists regarding the range in composition within or among grades. Consequently, adding value to cows may be accomplished by more accurately determining composition. Abbreviations: OTM, over 30 mo of age; UTM, under 30 mo of age; REA, rib-eye area 99 Can. J. Anim. Sci. Downloaded from pubs.aic.ca by Alberta Government Library on 03/08/13 For personal use only. 100 CANADIAN JOURNAL OF ANIMAL SCIENCE On the other hand, since the re-opening of the border to meat from cattle under 30 mo of age (UTM), a new category of youthful animals has been established by the industry. So called ‘‘blue-’’ or ‘‘red-’’ back carcasses (due to the rolling of blue or red ink down the backbone to identify them), they are graded as youthful carcasses, based on ossification (B50%), but, based on dentition, are deemed to exceed 30 mo of age (OTM). Unfortunately, physiological age, based either on dentition or ossification, is not accurately related to chronological age (Lawrence et al. 2001; Chaudhary et al. 2008) yet in the absence of recorded birthdates dentition has been used for international trade. While OTM carcasses may be falling into the more mature end of the youthful grade, it is unlikely there is any quality-based reason to discount the carcasses. Consequently, the objectives of this study were: (a) to benchmark the Canadian grading standards for cows compared with youthful carcasses (over/under 30 mo of age) for carcass traits and cutability; (b) to determine the potential to develop a predictive equation for lean content in cow carcasses. MATERIALS AND METHODS Handling and slaughtering of animals at a commercial abattoir followed the humane practices described by the Canadian Council on Animal Care (1993). Carcass Selection Carcasses representative of the four Canadian mature cow grades (D1, D2, D3, D4, n 21 of each grade) were selected at the time of grading from a commercial abattoir by a certified beef grader [Canada Gazette 1992; Canadian Beef Grading Agency (CBGA) 1998]. At the same time, physiologically youthful carcasses (B50% ossification) in yield grade Y1 (59% or more of lean) and quality grade A or AA (Canada Gazette 1992) were selected based on a dentition breakpoint of under 30 mo (UTM; n 18; no permanent incisors) and over 30 mo (OTM; n 18; two or more permanent incisors) of age. The expression UTM and OTM are acronyms used by the beef industry for physiologically youthful carcasses (B50% ossification). Following selection, additional grade data were recorded according to the Livestock and Poultry Carcass Grading Regulations (Canada Gazette 1992), including muscling score (determined on the basis of rib-eye length and width measurements), body-type (beef or dairy), ossification (percentage of ossification of spinal process at 11th and 12th thoracic vertebrae), grade fat (minimum backfat thickness in the 3rd quarter between the 12th and 13th rib interface), rib-eye area (REA; between the 12th and 13th rib interface), fat colour (yellow fat presence). Marbling scores were assessed using United States Department of Agriculture marbling standards (USDA 1989). Left sides were shipped to the Agriculture and Agri-Food Canada Meat Research Centre (Lacombe, AB) by way of refrigerated truck. Following delivery, carcasses were weighed and held in a carcass cooler at 28C with wind speeds of 0.5 m s 1 until 67 d post-mortem. Carcass Fabrication Left carcass sides were weighed (cold carcass weight) to determine cooler shrink loss and fabricated according to the Canadian Meat Council (1988) into nine wholesale cuts or primals (158 round, 181A sirloin butt, 173 shortloin, 193 flank, 113 chuck, 103 rib, 121 plate, 120 brisket, 117 fore shank). Later on, all bone-in cuts were further deboned and dissected in order to obtain the total body-cavity fat, subcutaneous fat and intermuscular fat, lean and bone. Body-cavity fat is considered fat located on inner side of the brisket, plate, ribs, loin, chuck, and inner and outer side of diaphragm; as well as kidney, pelvic and heart fat where it remains with the carcass. Subcutaneous fat (fat removed from outside or exposed side of the primal cut) was completely removed, leaving the cut without a fat layer. After removing bodycavity and subcutaneous fat, intermuscular fat was obtained by removing all fat found in seams between muscles. Trimmings higher than 90% lean were considered as lean tissue, whereas trimmings less than 90% lean were considered as fat tissue and sorted in their corresponding fat depot for each primal. Weights for all previously mentioned products of fabrication were transformed to relative content and expressed as grams per kilogram carcass side weight and grams per kilogram primal weight. Statistical Analysis Carcass data and cutout characteristics were analyzed using the MIXED model procedures of SAS software version 9.2 (SAS Institute, Inc. 2003). Analysis of variance was performed for completely randomized design model, having carcass grades (D1, D2, D3, D4, OTM, and UTM) as the main effect. The individual carcass was included as a random factor. Least squares means were separated (F test, PB0.05) using least significant differences generated by the PDIFF option. The degrees of freedom in the denominator were adjusted using the KenwardRoger procedure. Simple descriptive statistics (PROC MEANS) were computed for carcass traits and composition. Correlation analysis was performed to determine the relationship of carcass traits (yield factors) to the dissected lean yield proportion. The Snedecor criteria was used to qualify the values of r as high, moderate or low (high: ]0.7; moderate: between 0.5 and 0.7; low: 50.5). Variance inflation factors (VIF) and collinearity diagnostics (COLLINOINT) from the PROC REG procedure of SAS software (SAS Institute, Inc. 2003) were used to detect multicollinearity prior to stepwise regression analyses. In order to evaluate the precision and accuracy of the equation obtained for cows, the dissected vs. new predicted lean yield percentages were plotted, and a linear regression was computed. Also, this plot was contrasted against the dissected vs. predicted RODAS-GONZÁLEZ ET AL. * CANADIAN GRADE STANDARDS FOR COWS lean yield percentage determined using the existing lean algorithm previously developed for Canadian youthful carcasses (Canada Gazette 1992). Can. J. Anim. Sci. Downloaded from pubs.aic.ca by Alberta Government Library on 03/08/13 For personal use only. RESULTS AND DISCUSSION Carcass Traits Mature carcasses based on evaluation of the degree of ossification of the spinal processes and ribs, fall into the D grades (Canada Gazette 1992; CBGA 1998). Carcasses from bulls or stags of any age which show pronounced masculinity fall into the E grade. To qualify for the D1 grade, mature carcasses must have excellent muscling and be well finished with white to amber fat (B15 mm backfat). Mature carcasses with medium to excellent muscling or with yellow fat fall into the D2 grade. Mature carcasses which are deficient in muscling to the point of emaciation receive a D3 grade, whereas, over-fat mature carcasses with deficient to excellent muscling (15 mm backfat) receive a D4 grade. In the population studied, D1 and D4 carcasses were heavier (P B0.05) than the other D and both the UTM and OTM youthful grades (Table 1). The D2 and youthful grades had similar cold carcass weight; D3 grades had the lightest carcass weights. Furthermore, D4 grade carcasses had greater backfat depth (P B0.05) than the other D and youthful grades; D3 had the least backfat. The REA from UTM carcasses was the largest (P B0.05), followed by D1, OTM and D2. Carcass D3 and D4 grades had the smallest REA. As expected, OTM and UTM carcasses had the lowest (P B0.05) percentage of ossification of the spinal process at the 11th and 12th thoracic vertebrae compared with D carcass grades and no presence of yellow fat colour. Nevertheless, the D4 grade obtained the highest marbling scores (P B0.05; ‘‘Modest’’), followed by D1, D2, and OTM (‘‘Modest’’ to ‘‘Small’’), next by UTM 101 (‘‘Small’’) and finally D3 with the lowest marbling score (‘‘Traces’’). According to the 2001 Canadian Beef Quality Audit (Van Donkersgoed et al. 2001), top quality Canadian grade heifers and steers presented numerically heavier carcasses and larger REA than cows, but similar grade fat thickness. Also, the range in carcass traits and lean percentage in the present study were higher than those reported in the Canadian Beef Quality Audit for cows (Van Donkersgoed et al. 2001), which makes the present population ideal for developing lean yield prediction equations. In the 2001 audit, the average hot carcasses weight was 300 kg, REA was 75 cm2 and grade fat of 8 mm, with an average lean percentage of 56.6%. In contrast, in a study (Stelzleni et al. 2007) comparing feeding cull cows vs. youthful USDA Select steer carcasses, cull cows fed with a high energy ration before slaughter had smaller rib-eye area and medium muscle conformation than A-maturity USDA Select carcasses. However, the cull cows (especially beef-type) also had more marbling and backfat thickness. A-maturity USDA Select carcasses had more desirable carcass characteristics (brighter lean colour and whiter backfat) than cull cows that did not receive a high energy ration prior to slaughter. In the present study, the information about management history of the cows was unavailable. Regardless of feeding history, D1 and D2 carcasses had similar carcass characteristics to OTM and UTM carcasses, with the exception of higher marbling and ossification. Intramuscular fat is closely linked to fatty tissue development and is deposited over a long period of growth (Berg and Butterfield 1968; Robelin 1986); thus, cows are likely to have high levels of marbling as animals mature. Greater ossification has been shown to decrease tenderness (Smith et al. 1982, 1988; Hilton et al. 1998) and increased marbling has been shown to Table 1. Carcass traits from mature cattle quality grades and youthful gradesz groups Canadian grade D1 D2 D3 D4 OTM UTM Trait n 21 n 21 n21 n 21 n18 n 18 SEM P value Cold carcass weight (kg) Grade fat (mm)y Rib-eye area (cm2) Ossification (%)x Marbling scorew Yellow fat presence (%)v 359a 8.71b 90.1b 88.5b 517ab 0.00 331b 7.38bc 84.5bc 94.2ab 491bc 9.17 310c 0.43d 81.1c 96.4a 272e 6.67 359a 21.76a 78.5c 91.9ab 543a 4.17 334b 7.67b 89.7b 6.39c 448cd 0.00 325bc 6.06c 97.5a 0.00c 424d 0.00 5.85 0.54 2.46 2.61 18.9 B0.001 B0.001 B0.001 B0.001 B0.001 B0.001 z Canada beef grading system (Canada Gazette 1992). D1 to D4: Canadian quality grades for mature animals. OTMA/AA youthful physiologically and over 30 mo based on dentition. UTMA/AA youthful physiologically under 30 mo based on dentition. y Backfat thickness between the 12th and 13th ribs’ interface. x Percentage of ossification of spinal process at 11th and 12th thoracic vertebrae. w USDA marbling standards: 200299traces; 300399slight; 400499small; 500599 modest; 600699moderate; 700799 slightly abundant; 800899moderately abundant; 900999 abundant; 10001099very abundant. v Chi-square analysis indicated the distribution was different by carcass grades. ae Least squares means within a row lacking a common letter differ (PB0.05). Can. J. Anim. Sci. Downloaded from pubs.aic.ca by Alberta Government Library on 03/08/13 For personal use only. 102 CANADIAN JOURNAL OF ANIMAL SCIENCE improve palatability and consumer acceptance (Savell et al. 1987; Neely et al. 1998; Killinger et al. 2004). Opportunity to capitalize on the higher marbling content in these grades would likely require concomitant intervention strategies for tenderness. Based on ossification both UTM and OTM carcasses would fall into the same grade. However, to facilitate international trade, UTM and OTM carcasses are separated based on dentition. The present study indicates UTM and OTM carcasses were similar. The only differences between OTM and UTM were for grade fat and REA, where OTM grades presented thicker backfat and smaller REA compared with UTM carcasses (P B0.05). Although not significantly different (P 0.05), OTM carcasses had a numerically higher ossification percentage (6.39%) compared with UTM carcasses (0.00%). Carcass Cutout The D3 carcass grades had the highest proportion of lean (P B0.05) due to lower proportions of dissectible fat components (P B0.05; body cavity, subcutaneous, intermuscular and total), and despite a higher proportion of bone (P B0.05; Table 2). In contrast, D4 carcass grades had the lowest proportion of lean and bone; but much higher proportions of subcutaneous, intermuscular and total fat. The D1, D2, OTM and UTM had intermediate values for most of the dissectible components and were generally very similar in composition. No differences in dissectible tissue components were detected between OTM and UTM (P 0.05). Despite these differences in proportional yields when carcass weights were taken into account, the highest actual lean yield in absolute terms (kg) was found in the D1 (219.7 kg; data not shown) and the lowest in the D4 carcasses (198.5 kg), representing an average of 21.2 kg more lean meat in the D1 carcass grade. In monetary terms, this would equate to an additional revenue of CDN $99.85 (CDN $4.71 kg 1 cow trim 85%; Canfax 2012). Primal cut yields (Table 2) indicated D1, D2 and D4 carcass grades had higher proportions of sirloin butt and flank than the other carcass grades (P B0.05). In addition, OTM and UTM carcasses had higher proportions of brisket. The D4 carcass grades were mainly differentiated by higher proportions of short loin, rib and plate (P B0.05). The proportions of round and chuck were higher in D3 carcass grades; even though, UTM carcasses also had higher proportions of round. The proportion of fore shank was higher in D3, UTM and OTM carcass grades. Except for the proportion of lean in the fore shank where there were no statistical differences among grades (P 0.05; Table 3), the proportion of lean in most of the primals was higher in D3 carcass grades than the other grades (P B0.05), followed by D1, D2, OTM and UTM, and then D4. The UTM had a higher proportion of lean in the sirloin butt and short loin primals compared with other grades (P B0.05), but always ranked second after the D3 carcass grade. The proportion of Table 2. Carcass components and full dissection valuesz from mature cattle quality grades and youthful grades groups Canadian grade Carcass composition (g kg Total lean Total bone Total body cavity Total subcutaneous fat Total intermuscular fat Total fat D2 D3 D4 OTM UTM n21 n 21 n 21 n18 n 18 SEM P value 612b 171c 24.5b 65.0b 128b 217b 611b 182b 24.3b 60.1bc 123b 207b 693a 215a 12.4c 15.2d 64.6c 92.3c 553c 157d 29.5a 102a 159a 290a 606b 175bc 23.9b 64.2bc 131b 219b 623b 171c 23.8b 55.2c 127b 206b 7.04 3.13 1.13 3.03 4.43 7.53 B0.001 B0.001 B0.001 B0.001 B0.001 B0.001 241c 99.9a 71.5b 51.8bc 93.8b 278b 73.6b 54.0a 35.2b 241c 99.9a 64.6cd 55.0ab 93.5b 281b 71.3bc 56.2a 36.3b 262a 95.4b 63.5d 40.9d 89.4c 292a 68.3c 48.7b 38.5a 219d 100a 77.5a 58.6a 102a 275b 78.8a 55.2a 32.3c 252b 94.6b 68.8bc 49.4c 90.8bc 281b 70.1bc 53.9a 37.4ab 260a 85.4c 69.8b 48.6c 92.9bc 278b 68.0c 56.5a 39.2a 2.48 1.47 1.56 1.55 1.31 2.73 1.84 1.64 0.79 B0.001 B0.001 B0.001 B0.001 B0.001 0.001 0.001 0.011 B0.001 1 Primal cut yields (g kg1) Roundy Sirloin buttx Short loinx Flankx Riby Chuckw Platew Brisketw Foreshankw z D1 n 21 ) Yield calculated on base on carcasses side weight. OTMA/AA youthful physiologically and over 30 mo based on dentition. UTMA/AA youthful physiologically under 30 mo based on dentition. y Hind carcass section. x Middle carcass section. w Front carcass section. ad Least squares means within a row lacking a common letter differ (P B0.05). RODAS-GONZÁLEZ ET AL. * CANADIAN GRADE STANDARDS FOR COWS 103 Table 3. Dissection valuesz of individual primals (g kg1) from mature cattle quality grades and youthful grades groups Canadian grade Trait Can. J. Anim. Sci. Downloaded from pubs.aic.ca by Alberta Government Library on 03/08/13 For personal use only. y D1 D2 D3 D4 OTM UTM n 21 n 21 n 21 n 21 n 18 n 18 SEM P value Round Lean Bone BC fat SQ fat IM fat 703b 170bc 5.43b 60.6b 60.8a 687bc 178b 6.05ab 62.5b 66.4a 731a 200a 2.90c 24.2c 41.7b 676c 164c 6.77a 87.1a 65.7a 687bc 176b 5.75ab 64.9b 65.7a 695b 176b 5.70ab 60.5b 62.9a 5.93 3.09 0.44 3.55 2.52 B0.001 B0.001 B0.001 B0.001 B0.001 Sirloin Buttx Lean Bone BC fat SQ fat IM fat 607cd 161c 57.0ab 94.8b 80.5a 594d 175b 62.1ab 94.7b 74.3a 702a 207a 29.5c 16.3d 46.4b 527e 150c 64.6a 175a 83.1a 620c 162c 56.4ab 82.0b 80.2a 650b 156c 54.2b 63.9c 75.9a 8.34 4.24 3.60 5.91 3.79 B0.001 B0.001 B0.001 B0.001 B0.001 Short Loinx Lean Bone BC fat SQ fat IM fat 565c 177c 91.9b 127b 39.1a 569c 201b 81.8b 109cd 39.6a 663a 252a 47.4c 25.0e 13.2b 468d 152d 108a 226a 45.6a 576c 176c 82.6b 117bc 48.4a 606b 175c 82.7b 92.8d 44.4a 9.22 5.67 5.40 6.38 3.53 B0.001 B0.001 B0.001 B0.001 B0.001 Flankx Lean Bone IM fat 522b 16.5bc 462b 525b 18.7b 458b 724a 29.8a 246c 400c 11.8c 588a 504b 14.0bc 482b 529b 12.7c 459b 12.28 1.77 12.84 B0.001 B0.001 B0.001 Ribx Lean Bone BC fat SQ fat IM fat 545b 210c 17.6b 95.0b 133b 549b 228b 18.3ab 85.1b 119b 655a 276a 9.63c 11.8c 48.0c 466c 186d 20.4ab 155a 174a 549b 210c 20.9a 95.1b 125b 567b 204c 20.8a 83.4b 125b 8.64 4.49 1.12 5.18 6.06 B0.001 B0.001 B0.001 B0.001 B0.001 Chuckw Lean Bone BC fat SQ fat IM fat 652b 164bc 10.1a 40.3b 133bc 661b 172b 10.4a 35.4b 121c 723a 194a 5.69b 7.68c 70.5d 615c 156d 10.0a 57.9a 162a 645b 167bc 10.1a 42.0b 136bc 658b 159.6cd 10.1a 35.3b 137b 7.00 3.14 0.94 2.45 5.35 B0.001 B0.001 0.003 B0.001 B0.001 Platew Lean Bone BC fat SQ fat IM fat 516b 156b 74.6a 73.9b 180bc 534b 165b 77.5a 71.6b 152c 656a 220a 39.6b 16.8d 67.0d 454c 122c 89.7a 103a 231a 507b 160b 78.8a 72.5b 182bc 518b 162b 89.7a 42.8c 187b 10.73 6.06 5.69 5.77 10.59 B0.001 B0.001 B0.001 B0.001 B0.001 Brisketw Lean Bone BC fat SQ fat IM fat Lean Bone IM fat 484b 180c 17.9a 113ab 205b 487 394bc 119b 487b 208b 16.3a 92.9b 196b 484 398b 118b 583a 265a 11.1b 26.6c 115c 491 431a 77.5c 441c 172c 20.0a 135a 232a 478 377c 145a 467bc 186c 19.0a 116ab 212ab 460 404b 137a 487b 169c 15.6ab 124a 205b 488 396bc 116b 11.09 7.32 1.91 9.84 8.13 7.67 7.25 6.00 B0.001 B0.001 0.018 B0.001 B0.001 0.067 B0.001 B0.001 Fore Shankw z Yield calculated on base on primal weight. BC, body cavity; SQ, subcutaneous; IM, intermuscular. OTMA/AA youthful physiologically and over 30 mo based on dentition. UTMA/AA youthful physiologically under 30 mo based on dentition. y Hind carcass section. x Middle carcass section. w Front carcass section. ad Least squares means within a row lacking a common superscript letter differ (PB0.05). bone was higher in the D3 carcass grade in all primals (P B0.05), and D2 carcasses ranked second with higher proportion of bone in the sirloin butt, short loin, rib and brisket (P B0.05). In contrast, D4 carcasses had the lowest proportion of bone (P B0.05). For the fat depot proportions, D3 carcasses had the lowest proportion of dissectible fat depots (P B0.05; body cavity, subcutaneous, and intermuscular fat) with respect to the other grades. On the other hand, even though D4 carcass grades had higher proportions of total fat (P B0.05), for some fat depots, dependent on primal, the proportions were similar to D1, D2, OTM and UTM; for example, body cavity in the sirloin butt, chuck, rib, plate and brisket, as well as intermuscular fat in round, sirloin butt, and short loin (P 0.05). Can. J. Anim. Sci. Downloaded from pubs.aic.ca by Alberta Government Library on 03/08/13 For personal use only. 104 CANADIAN JOURNAL OF ANIMAL SCIENCE To understand the value of mature cows in the market place according to the types of carcases found in these grade classes, the lean meat yield in absolute terms (kg) can be estimated. For example, in the D1 grade with a carcass weight of 350 kg, the round would yield approximately 59 kg of lean [e.g. 350 kg 0.241 (Table 2) 0.703 (Table 3) 59 kg lean]; conversely, a D4 cow that weighed the same would yield 52 kg of lean (e.g. 350 kg 0.219 0.676 52 kg lean). Consequently, packers can use this information make improved decisions regarding the kind of cow carcass grades needed for merchandisable cuts or for manufacturing beef products (i.e., ground beef). At the same time, producers can increase their revenue through cow management and marketing improvements to supply the mature cows that meet market needs. Stelzleni et al. (2007) reported beef cull cows fed a low-energy diet had similar proportions of lean and fat to USDA Select A-maturity steer, but had higher lean and lower fat proportions than cull cows (either beef or dairy cows) fed a high-energy diet. Most Canadian studies have been conducted on youthful rather than mature female animals and have denoted certain carcass composition disadvantage when compared with males. Fredeen et al. (1981) reported higher carcass lean content for A1 grades from steers than heifers, but these sex differences gradually diminished as yield class increased (A1 to A4). Jones et al. (1987) also indicated that for A1 carcasses, steers had a higher lean proportion than heifers; however, A2 carcasses from both sexes had similar lean content with or without adjustment to the same level of backfat thickness. In both studies, the differences in lean proportion were due to a higher proportion of trimmed fat on heifer carcasses. Cull Cow Predictive Equation for Lean For cull cow carcass traits (Table 4), a wide range of variation was observed in the rib-eye width (CV 43.57%) and overall muscle score (CV 39.78%), while grade fat, fat class, REA, rib-eye length, marbling and ossification presented moderate to low ranges of variation (CV 22 to 12%). Cold carcass weight showed lower variation (CV 7.27%). Overall, although the variation in total dissected lean content ranged from 52 to 77%, the CV was relatively low (4.32%; data not shown). A small variation in body measurements (lengths and areas) and a wide variation in the indicators of body fat have been reported by other authors (Abraham et al. 1980; Atencio-Valladares et al. 2008) for cattle. The findings of the current study agree with those of Johnson and Rogers (1997); when working with mature cows, they found a moderate to low range of variation in most of carcasses traits, with the exception of marbling (CV of 56%). Similarly, O’Mara et al. (1998) reported higher variability for marbling score (44.76%). The correlation coefficients for carcass traits and lean yield percentage are presented in Table 4. According to Snedecor criteria, grade fat, fat class and marbling score variables could be good estimators of lean yield percentage (LEAN) (r ]0.700 and P B0.001). These variables had negative associations, indicating that increases in fat deposition would result in lower LEAN. On the other hand, cold carcass weight had moderate negative association (r0.452 and P B0.001), while REA (cm2) and rib-eye length presented low positive association with LEAN (r0.226 and 0.239, respectively and P B0.05). A positive trend (r 0.200 and P 0.067) was observed for ossification scores. Rib eye width (mm) and Table 4. Independent variable range and their correlation with dissected lean yield in the population of cull cows Lean yield percentage correlation z Carcass traits N Mean STD Cold carcass weight (kg) Grade fat (mm)y Fat classx Rib eye area (cm2) Rib eye width (mm) Rib eye length (mm) Muscle scorew Marblingv Ossificationu 84 84 76 84 76 76 76 84 84 346 9.59 4.58 83.6 45.1 67.5 2.45 456 92.7 25.2 2.05 0.83 10.2 20.0 13.0 0.97 95.5 13.2 z CV z 7.27 21.4 18.1 12.2 43.6 19.2 39.8 21.0 14.2 Min Max rt P value 307 6.25 3.00 66.3 25.4 38.1 1.00 310 54.0 391 14.0 6.00 106 76.2 76.2 4.00 671 100 0.452 0.842 0.822 0.226 0.047 0.239 0.129 0.725 0.200 B0.001 B0.001 B0.001 0.038 0.686 0.038 0.267 B0.001 0.067 STD, standard deviation; CV, coefficient variation. Backfat thickness between the 12th and 13th ribs’ interface. x Determined on the basis of back fat thickness: 3 6 or 7 mm; 614 or 15 mm. w Determined on the basis of rib-eye length and width measurements: 1B141 mm length and B64 mm width; 4150 length and 71 mm width. v USDA marbling standards: 200299traces; 300399slight; 400499small; 500599modest; 600699 moderate; 700799slightly abundant; 800899moderately abundant; 900999 abundant and 10001099very abundant. u Percentage of ossification of spinal process at 11th and 12th thoracic vertebrae. t r, Pearson correlation coefficient. y Can. J. Anim. Sci. Downloaded from pubs.aic.ca by Alberta Government Library on 03/08/13 For personal use only. RODAS-GONZÁLEZ ET AL. * CANADIAN GRADE STANDARDS FOR COWS 105 and ossification slightly improved the model (R2 0.81 and 0.83, respectively). However, when ossification was included as a fourth variable, it reduced the Mallow’s coefficient (Cp 4.31 vs. 7.44), thus improving the model accuracy. Consequently, based on the current dataset the best equation to predict LEAN in cull cows was: LEAN 59.2131 (grade fat 0.3869)(REA 0.0946) (marbling 0.0152)(ossification 0.0515). The Canadian youthful lean yield algorithm was not designed to accommodate mature carcasses, only youthful carcasses in Canada Prime and A quality grades. However, in this study a new equation for mature cow carcasses was developed and was compared with the results obtained using the yield algorithm developed for youthful carcasses [(lean% 63.651.05 (muscle score) 0.76 (grade fat)]. Both equations (the new predictive LEAN equation for cows and the currently used predictive LEAN equation for youthful animals) were contrasted (Fig. 1). As expected, the results indicate that the new lean yield equation for cows was more precise in predicting the actual lean yield percentage (R2 0.83) than the equation used for youthful animals (R2 0.70). In agreement with other studies (Kauffman et al. 1975; Abraham et al. 1980; Savell et al. 1987; Smith et al. 1988; Johnson and Rogers 1997; Neely et al. 1998; O’Mara et al. 1998; Killinger et al. 2004; AtencioValladares et al. 2008), grade fat, REA and marbling score were included in multiple regression equations for predicting cutability and were useful predictors in the current study. Johnson and Rogers (1997) reported the best equation for predicting the yield of whole muscle cuts from mature cow carcasses had three variables (hot carcass weight, rib-eye area and marbling) but, the percentage of total variation explained in their study was relatively low (R2 0.58). Interestingly, their equation contained hot carcass weight, which invariably is collinear with the proportion of lean per unit of carcass weight. O’Mara et al. (1998) used USDA yield and quality grade factors in cows to predict LEAN content. The best carcass traits for their LEAN prediction model included adjusted preliminary yield grade, rib-eye area adjustment, marbling score and lean maturity, resulting in a high R2 (0.79). Given the differences in cattle populations, comparison with other foreign prediction muscle score were not significantly correlated with LEAN (P 0.05). Some researchers (Kauffman et al. 1975; Abraham et al. 1980; Johnson and Rogers 1997; O’Mara et al. 1998) have reported that carcass traits for evaluating fatness (backfat thickness and marbling) from mature or young animals had the highest simple correlation to LEAN. On the other hand, Johnson and Rogers (1997) also indicated that bone maturity from cows was not associated with LEAN, while REA and carcass weight had a low association. These findings agree with the results of current study. Other researchers (Crouse et al. 1975; Abraham et al. 1980; Atencio-Valladares et al. 2008) have indicated that carcass weight has little predictive power for LEAN. Although, ossification was not associated with total LEAN proportion, during the correlation analysis, positive correlation between ossification and lean proportion in some primal cuts (chuck, rib and plate, results not shown) was detected. In consequence, when a tendency (P 0.07) was detected in the relationship between ossification and total LEAN proportion, it was considered a rational decision to include it in the stepwise regression analysis. Additionally, during post-natal development, bone has slow growth and maturity rate than muscle and fat (Berg and Butterfield 1968; Van Sickle 1985). However, the implications of relative tissue growth for evaluation of carcass composition are that bone percentage decreased continuously in relation to the other tissue; thus, the stage of development at slaughter can influence carcass composition (Berg and Butterfield 1968). Thus, it can be assumed that more ossification (50100%) could lead to a higher muscle:bone ratio. Due to a lack of association (rib-eye width and muscle score) some variables were discarded and the rest of the traits were considered as predictors of LEAN (they did not present multicollinearity). The better predictive equation was selected based on the highest determination coefficient (R2) and the lowest Mallow’s coefficient (Cp) (Table 5). Stepwise regression included grade fat, rib-eye area, marbling and ossification as predictors of LEAN. Grade fat alone contributed the most to prediction of LEAN (R2 0.70; P B0.001). Further, stepwise inclusion of marbling score improved the model (R2 0.78), whereas inclusion of REA (cm2) Table 5. Regression equations for predicting carcass lean yield percentages Coefficient Equation Intercept GFAT MARB REAz OSSIz R2y Cpy P value 1 2 3 4 66.9619 71.7917 64.6708 59.2131 0.5815 0.4416 0.4074 0.3869 0.0136 0.0891 0.0946 0.0151 0.0152 0.0515 0.70 0.78 0.81 0.83 48.83 17.10 7.45 4.31 B0.001 B0.001 0.001 0.026 z z z GFAT, grade fat; REA, rib eye area; MARB, marbling score; OSSI, ossification. R2, coefficient of determination; Cp, Mallow’s coefficient. y Can. J. Anim. Sci. Downloaded from pubs.aic.ca by Alberta Government Library on 03/08/13 For personal use only. 106 CANADIAN JOURNAL OF ANIMAL SCIENCE Within the mature carcass grades, D1 and D4 cow carcasses had the most marbling, slightly lower REA, and less yellow fat presence; thus, meat from these carcasses may be used in value added products with inclusion of tenderness interventions. The D3 carcasses have values for production of manufacturing beef due to their overall low fatness resulting in high proportional lean meat yield. The present data show the Canadian grade classes for mature cattle do not do an adequate job of segregating carcasses on lean yield based on consideration of limited carcass characteristics. Consequently, the equation to predict LEAN developed in the present study explained over 83% of the variation, indicating that there may be an opportunity to develop a yield grade for cows and more precisely define their carcass value. ACKNOWLEDGEMENTS This study was funded by Agriculture and Agri-Food Canada as part of their BSE Recovery Strategy. The authors sincerely acknowledge the cooperation of XL Beef and assistance for in-plant grading provided by Mr. Fred Taylor. We thank the Meat Processing staff and Mr. Stan Landry from the Lacombe Research Centre for their technical assistance. Fig. 1. Contrasting the new predictive lean yield equation for cull cows vs. the predictive equation from Canada lean yield grade. The new predictive equation for cull cows is: LEAN 59.2131 (grade fat0.3869)(REA0.0946) (marbling 0.0152)(ossification 0.0515). The predictive equation for youthful animals: LEAN 63.651.05 (muscle score) 0.76 (grade fat). equations could lead to inaccurate predictions (Lee et al. 2005). Furthermore, for illustration purposes only, the new equation was tested on the same population used to develop the model. Thus, the next steps would require validation of the developed prediction equation for Canadian cow carcasses using a separate commercial population. CONCLUSIONS In the present study, D1 carcasses were comparable with youthful carcasses (UTM and OTM). The addition of a breakpoint based on dentition to facilitate international trade results in carcass classes that had some distinguishing characteristics. 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