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ATP assay kit
Catalog No: BC0157
Method: UV colorimetry
Size: 100T/50Samples
Introduction:
ATP (adenosine 5’-triphosphate, ATP) is the most basic carrier in biological energy conversion in
the body. The change of its content will directly affect energy metabolism of different organs. As
the most important energetic molecule , ATP plays an important role in the procession of cell
physiology and pathology. Change of ATP level can affect the function of many cells. Usually
ATP level will decrease when cells are under a state of apoptosis, necrosis or toxicity. However,
high glucose stimulation can increase the ATP level in cell. Usually a decrease in the level of ATP
shows damage or fall in mitochondrial function.ATP assay kit can be used to detect the ATP level
in the red blood cells or tissue.
Features：
1.Quick and easy: it can measure 50 samples in 2 hours.
2.Good stability: it can be stored for three months at 2 - 8℃.
3.Good reproducibility: coefficient of variation CV = 1.7%.
4.Recovery test: X = 102%.
5.Less affected by external factors: less interference factors and good repeatability.
6.Wide testing range: it can measure tissue and other samples.
Experimental instrument:
Test tube, micropipettor, vortex mixer, 37℃ water bath/gas bath, low-speed centrifuge, visible
spectrophotometer (636nm)
Applicable scope:
This kit can measure ATP content of all kinds of animal whole blood, red blood cells, tissue,
culture cells and other samples.
Operation procedures:
1mmol/L standard
solution(μ l)
Sample(μ l)
Reagent one: substrate
solution Ⅰ(μ l)
Reagent two: substrate
solution Ⅱ(μ l)
Reagent three:
accelerant(μ l)
Double distilled
Blank tube
Standard tube
30
30
Measured tube
Contrast tube
30
30
100
100
100
100
200
200
200
200
30
30
30
ELABSCIENCE BIOTECHNOLOGY CO.,LTD
30
FOR RESEARCH USE ONLY
Elabscience
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water (μ l)
Mix, water bath for 30 minutes at 37℃
Reagent four:
50
50
50
50
precipitant(μ l)
Centrifuge for 5 minutes at 4000 r/m after mixing fully, take 300μl supernatant to measure
300
300
300
300
Supernatant sample(μ l)
Reagent five:
500
500
500
500
colorimetric solution(μ l)
Mix, still for 2 minutes at room temperature
Reagent six:
500
500
500
500
stop solution(μ l)
Mix, still for 5 minutes at room temperature. 636nm wavelength, 0.5cm diameter, zero with
double-distilled water, measure the OD values of each tube.
Calculation formula
①Computational formula for red blood cells:
Concentration of ATP(μ mol/ Hbg)
Concentration of standard
（1000μ mol/L） Dilution factor of sample Concentration of hemoglobin(Hbg/L)
②Computational formula for tissue, cell
Concentration of ATP(μ mol/gprot)
Concentration of standard
（1000μ mol/L） Dilution factor of sample Concentration of protein in sample(gprot/L)
ELABSCIENCE BIOTECHNOLOGY CO.,LTD
FOR RESEARCH USE ONLY