CleaningandDisinfectionProtocolforFungi,YeastandMolds Thisdocumenthasbeendevelopedinaccordancewithcurrentapplicableinfectioncontroland biosecurityguidelines.Itisintendedforuseasaguidelineonly.Atnotimeshouldthisdocumentreplace existingdocumentsestablishedbythefacilityunlesswrittenpermissionhasbeenobtainedfromthe responsiblefacilitymanager. PREFACE Fungiisthenamegiventoastrangegroupoflivingthingsincludingmushrooms,toadstools,moldsand thousandsofotherweirdandwonderfulthings.Fungiareneitherplantnoranimal,buthavesome characteristicsofeach.Theycannotmoveaboutlikeananimal,doconsumeorganicmatter,haveno chlorophyllasdoplants,andcannotmanufacturetheirownenergy.Theyhaveatruenucleusintheir cellsandareabletosexuallyreproducebycombininglikestrainsofnucleus.Theycanalsoreproduceby sporessimilartosomeofthemoreprimitiveplants.Generally,themajorityofcommonfungi,yeasts andmoldsarenotaconcerntosomeonewhoishealthy.However,therearesomeindividuals, especiallythosewhohaveexistinghealthconcerns,whomaybesensitivetoexposureofthese organisms. Thefollowingtableprovidesexamplesofpathogenicfungi,yeastsandmoldsforbirds,mammalsand primates. Aspergillusbrasiliensis Microsporumcanis Pneumocystismurina Aspergillusfumigatus Microsporumgypseum Trichophytonequinum Candidaalbicans Pneumocystiscarinii Trichophyton mentagrophytes Encephalitozooncuniculi(ECUN) PREPARATION Transmissionoffungi,yeastandmoldsisgenerallyacquiredfromsporesfoundinsoilorvegetationand eitheringestedorinhaled.Animal-to-Animaltransmissionisnotofgeneralconcernwithfungiwiththe exceptionoforganismsthatcauseringwormwherethefungiarefoundontheskinandcanbe transmittedviadirectcontact.Appropriatepersonalprotectionshouldbetakenforthoseresponsible forthedecontaminationofanimalpremises.Appropriatebiosecuritypracticesshouldbeapplied, includinglimitingtheamountofaerosolsgeneratedanddisturbancetodust/soilintheareatobe cleanedanddisinfected. PROTECTIVEBARRIERS Appropriatepersonalprotectionshouldbetakenforthoseresponsibleforthedecontaminationofthe areaandmayincludethefollowing: 1. Disposablegloves.Glovesshouldbechangedasrequired(whentorn,whenhandsbecomewet insidethegloveorwhenmovingbetweenanimalhousingareas) 2. Householdglovescanbeworn,buttheymustbediscardedwhenthecleaningiscomplete. 3. ProtectiveEyewear(goggles,faceshieldormaskwitheyeprotection)asappropriatebasedon tasktobecompleted 4. Masks(surgicalorproceduralmaskssufficient)asneeded 5. Gownsasneeded PRODUCTS AllAcceleratedHydrogenPeroxide®(AHP®)disinfectant-cleanerproductshavebeenapprovedforsale asadisinfectantbyeitherHealthCanadaortheUSEnvironmentalProtectionAgency(EPA)andcarry eitheraDINorEPAregistrationnumberindicatingtheirapprovalforsaleasadisinfectant. AHP®disinfectantsareregisteredasaHospitalGradeDisinfectantwhichdenotesthattheproductshave beenprovenefficaciousagainstthethreemainsurrogatebacteriadesignatedbothHealthCanadaand theEPAforBactericidalactivity;Staphylococcusaureus,PseudomonasaeruginosaandSalmonella enterica(formerlyknownasSalmonellacholeraesuis).AHP®disinfectantshavealsobeentestedagainst awiderangeofpathogenicfungi,yeastsandmoldsincludingMicrosporum,Trichophyton,Candidaand Aspergilluswithcontactstimesof30secondsto10minutesdependingontheformulationand/or dilutionused. ProductName Prevail™ Rescue™ Intervention™ Market CompanionandFarmAnimal CompanionAnimal FarmAnimal Country Canada USA USA RECOMMENDEDPROCEDURESFORCLEANINGANDDISINFECTIONOFANIMAL CAREEQUIPMENT Contaminatedanimalcareequipmentshouldbeclearlyidentifiedandkeptseparatefromclean equipment.Animalcareequipmentinclude:bloodpressurecuffs,stethoscopes,thermometers, handlingorrestrainingequipment,feedingandwateringappliances,etc. 1. UsetheAHP®solutiontoremovesoilandbodymaterials(e.g.blood,organicsoils)fromheavily soileditems 2. ApplyAHP®solutionevenlyoversurfaceensuringthatallsurfacesarethoroughlywet.Items thatcanbeimmersedmaybeplacedintoacontainerofAHP®solution. 3. AllowtheitemstoremainincontactwiththeAHP®solutionfortheappropriatecontacttime (refertothelabeloftheAHPsolutionbeingused). 4. Afterthecontacttimehasbeenachievedremoveanyexcesssolutionwithacleancloth,sponge orpapertowel. 5. Itemsthathavebeenimmersedshouldberemovedfromthesolutionaftertheappropriate contacttime,rinsedwithpotablewaterandallowedtoairdry. RECOMMENDEDPROCEDURESFORCLEANINGANDDISINFECTIONOFKENNELS ANDCAGES: TheAHP®technologyisdesignedtobebothanexcellentcleanerandeffectivedisinfectantandcanbe usedinplaceofadegreaserordetergent. 1. 2. 3. 4. Removeallanimalsandfeedfromtheareatobecleanedanddisinfected. Removealllitterandfecalmatterfromfloors,wallsandsurfacesofkennelsorcages. Emptyorcoverallfeedingandwateringappliances. ThoroughlycleanallsurfaceswithsoaporadetergentoradilutedsolutionofAHP®andrinsewith water. 5. ApplytheAHP®solutiontofloors,wallsandothersurfacesofthekennelsorcagesensuringthe surfacesarethoroughlywetandremainwetfortheappropriatecontacttime. 6. Allowsurfacestodrybeforereintroducinganimalsbackintothekennelsorcages. 7. Cleananddisinfectallequipmentusedtoremovelitterandfecalmattersuchasforks,shovelsand scrapers. RECOMMENDEDPROCEDURESFORCLEANINGANDDISINFECTIONOFANIMAL HOUSINGFACILITIES: TheAHP®technologyisdesignedtobebothanexcellentcleanerandeffectivedisinfectantandcanbe usedinplaceofadegreaserordetergent. 1. Removeallanimalsandfeedfromtheareatobecleanedanddisinfected. 2. Removealllitter,beddingandmanurefromfloors,wallsandsurfacesofcages,stallsorotheranimal handlingareas. 3. Emptyorcoverallfeedingandwateringappliances. 4. ThoroughlycleanallsurfaceswithsoaporadetergentoradilutedsolutionofAHP®andrinsewith water. 5. ApplytheAHP®solutiontofloors,wallsandothersurfacesofthecages,stallsorotheranimal handlingareasensuringthesurfacesarethoroughlywetandallowtoremainwetforthe appropriatecontacttime. 6. Allowsurfacestodrybeforereintroducinganimalsbackintothekennelsorcages. 7. Cleananddisinfectallequipmentusedtoremovelitter,beddingandmanuresuchasforks,shovels andscrapers.
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