Examples for Medium Exchange Frequency in CelCradle-500
Cell Line
rCHO
rCHO
Culture
Medium
HyQ-PFCHO
α-MEM/
10%FBS
Initial
Cell
Seeding
Day of
Medium
Exchange
Medium
Formula
Notes
3, 5, 7, 9, 11,
13, 17,…
7 g/L Gluc
6 mM Gln
2.2 g/L
NaHCO3
1. CO2 switch from 5% to
0% at Day 3
2. NaHCO3 increased to
3.7g/L at Day 9 and 4.4
g/L at Day 13
3. B_H = 30 mins
3, 6, 8, 10,
12, 14, 15,
16,…
3 g/L Gluc
4 mM Gln
2.2 g/L
NaHCO3
1. CO2 switch from 5% to
0% at Day 3
2. NaHCO3 increased to
3.7g/L at Day 9 and
4.4 g/L at Day 13
3. B_H = 30 mins
3, 5, 7, 9, 11,
13, 15,…
4 g/L Gluc
4 mM Gln
3.7 g/L
NaHCO3
1. CO2 switch from 5% to
0% at Day 3
2. NaHCO3 increased to
3.7g/L at Day 9 and
4.4 g/L at Day 13
3. B_H = 30 mins
1×108
3, 4, 5, 6, 7…
4 g/L Glu
4 mM Gln
2.2 g/L
NaHCO3
CO2 switch from 5% to 0%
at Day 5
1×108
3, 4, 5, 6,
7,…
3 g/L Gluc
4 mM Gln
2.2 g/L
NaHCO3
CO2 switch from 5% to 0%
at Day 6
4.5 g/L Gluc
4 mM Gln
2.2 g/L
NaHCO3
SF900 II + 7 ml
1 M Bis-Tris
(pH=6.5)
2×108
1×108
Hybridoma
IMDM/
10%FBS
BHK
DMEM/
5%FBS
VERO
M199/
5%FBS
HEK293
DMEM/
10%FBS
1.5×108
3, 5, 7, 9, 11,
12,…
Sf-9
SF 900 II
(Gibco)
1.5×108
7,10,13,16,…
Hi-5
Express
Five
1.5×108
4,6,8,10,…
1×108
Add Bis-Tris to maintain
pH in 5.8-6.5 at Day3, 6,
8, 9, 11,12, 14, 15
Add Bis-Tris to maintain
pH in 5.8-6.5 at Day 2, 3,
5, 7, 9
1|Examples for Medium Exchange Frequency in CelCradle -500
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2|Examples for Medium Exchange Frequency in CelCradle -500