chapter 21
appendix
applications and troubleshooting
Overview for Electrophoresis and Western Blotting, continued
Apparent Molecular Weights of SeeBlue® and MultiMark® in Different Buffers
SeeBlue®
Pre-Stained Standard
Tris-Glycine
Gels
(4-20%)
Tricine
Gels
(10-20%)
NuPAGE® Bis-Tris
(MES) Gels
(4-12%)
210kDa (1)
Myosin (1)
188kDa (1)
NuPAGE® Bis-Tris
(MOPS) Gels
(4-12%)
191kDa (1)
NuPAGE®
Tris-Acetate Gels
(3-8%)
210kDa (1)
250kDa (1)
BSA (2)
78kDa (2)
Glutamic Dehydrogenase (3)
98kDa (2)
55kDa (3)
Alcohol Dehydrogenase (4)
64kDa (3)
45kDa (4)
Carbonic Anhydrase (5)
50kDa (4)
34kDa (5)
Myoglobin (6)
36kDa (5)
23kDa (6)
30kDa (6)
16kDa (7)
62kDa (2)
49kDa (3)
38kDa (4)
28kDa (5)
Aprotinin (8)
Insulin (9)
39kDa (4)
71kDa (2)
18kDa (6)
14kDa (7)
16kDa (7)
28kDa (5)
55kDa (3)
19kDa (6)
7kDa (8)
6kDa (8)
4kDa (9)
51kDa (3)
4kDa (9)
6kDa (8)
14kDa (7)
41kDa (4)
3kDa (9)
21.2
appendix
Lysozyme (7)
64kDa (2)
MultiMark®
Multi-Colored Standard
Tris-Glycine
Gels
(4-20%)
Myosin (1)
250kDa (1)
Phosphorylase B (2)
148kDa (2)
Tricine
Gels
(10-20%)
208kDa (1)
105kDa (2)
NuPAGE® Bis-Tris
(MES) Gels
(4-12%)
185kDa (1)
NuPAGE® Bis-Tris
(MOPS) Gels
(4-12%)
188kDa (1)
NuPAGE®
Tris-Acetate Gels
(3-8%)
209kDa (1)
98kDa (2)
97kDa (2)
Glutamic Dehydrogenase (3)
53kDa (3)
Carbonic Anhydrase (4)
60kDa (3)
Myoglobin (Blue) (5)
42kDa (4)
Myoglobin (Red) (6)
30kDa (5)
Lysozyme (7)
22kDa (6)
34kDa (4)
6kDa (8)
4kDa (9)
52kDa (3)
31kDa (4)
19kDa (6)
17kDa (5)
33kDa (4)
13kDa (7)
11kDa (7)
17kDa (7)
Insulin (9)
111kDa (2)
23kDa (5)
17kDa (6)
Aprotinin (8)
52kDa (3)
7kDa (8)
6kDa (8)
4kDa (9)
3kDa (9)
21kDa (6)
19kDa (5)
52kDa (3)
12kDa (7)
34kDa (4)
Important Licensing Information
These products may be covered by one or more Limited Use Label Licenses (See Catalog number/Label License Index and Label Licenses in Appendix). By use of these products you accept
the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only. CAUTION: Not intended for human or animal diagnostic or therapeutic uses.
882
800 955 6288
applications and troubleshooting
appendix
chapter 21
Overview for Electrophoresis and Western Blotting, continued
Apparent Molecular Weights of SeeBlue® Plus2 and Mark12™ in Different Buffers
SeeBlue® Plus2
Pre-Stained Standard
Tris-Glycine
Gels
(4-20%)
Tricine
Gels
(10-20%)
NuPAGE® Bis-Tris
(MES) Gels
(4-12%)
NuPAGE® Bis-Tris
(MOPS) Gels
(4-12%)
NuPAGE®
Tris-Acetate Gels
(3-8%)
Myosin (1)
250kDa (1)
188kDa (1)
210kDa (1)
191kDa (1)
210kDa (1)
Phosphorylase B (2)
148kDa (2)
98kDa (2)
105kDa (2)
BSA (3)
98kDa (3)
Glutamic Dehydrogenase (4)
64kDa (4)
Alcohol Dehydrogenase (5)
97kDa (2)
78kDa (3)
62kDa (3)
55kDa (4)
49kDa (4)
45kDa (5)
64kDa (3)
38kDa (5)
50kDa (5)
51kDa (4)
34kDa (6)
Carbonic Anhydrase (6)
111kDa (2)
28kDa (6)
71kDa (3)
36kDa (6)
Lysozyme (8)
17kDa (7)
17kDa (7)
16kDa (8)
39kDa (5)
55kDa (4)
14kDa (8)
22kDa (7)
28kDa (6)
Aprotinin (9)
7kDa (9)
6kDa (9)
4kDa (10)
3kDa (10)
41kDa (5)
appendix
16kDa (8)
19kDa (7)
6kDa (9)
Insulin (10)
Mark12™
Unstained Standard
4kDa (10)
Tris-Glycine
Gels
(4-20%)
Tricine
Gels
(10-20%)
NuPAGE® Bis-Tris
(MES) Gels
(4-12%)
14kDa (8)
NuPAGE® Bis-Tris
(MOPS) Gels
(4-12%)
NuPAGE®
Tris-Acetate Gels
(3-8%)
Myosin (1)
200kDa (1)
β-galactosidase (2)
Phosphorylase B (3)
BSA (4)
116.3kDa (2)
97.4kDa (3)
66.3kDa (4)
200kDa (1)
200kDa (1)
116.3kDa (2)
97.4kDa (3)
66.3kDa (4)
55.4kDa (5)
116.3kDa (2)
97.4kDa (3)
66.3kDa (4)
55.4kDa (5)
Glutamic Dehydrogenase (5)
Lactate Dehydrogenase (6)
36.5kDa (6)
36.5kDa (6)
31kDa (7)
31kDa (7)
21.5kDa (8)
21.5kDa (8)
Lysozyme (9)
Aprotinin (10)
116.3kDa (2)
97.4kDa (3)
200kDa (1)
36.5kDa (6)
31kDa (7)
55.4kDa (5)
116.3kDa (2)
97.4kDa (3)
21.5kDa (8)
Carbonic Anhydrase (7)
Trypsin Inhibitor (8)
200kDa (1)
66.3kDa (4)
55.4kDa (5)
14.4kDa (9)
36.5kDa (6)
14.4kDa (9)
31kDa (7)
6kDa (10)
21.5kDa (8)
3.5kDa (11)
2.5kDa (12)
14.4kDa (9)
6kDa (10)
14.4kDa (9)
6kDa (10)
Unresolved
insulin
66.3kDa (4)
55.4kDa (5)
6kDa (10)
3.5kDa (11)
2.5kDa (12)
36.5kDa (6)
31kDa (7)
Insulin B Chain (11)
Insulin A Chain 12)
Important Licensing Information
These products may be covered by one or more Limited Use Label Licenses (See Catalog number/Label License Index and Label Licenses in Appendix). By use of these products you accept
the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only. CAUTION: Not intended for human or animal diagnostic or therapeutic uses.
883
www.invitrogen.com
21.2
Myoglobin (7)