Vector Element
CMV Promoter
cPPT
WRE
TurboGFP
IRES-puro
AMPr
5'LTR
pUC ori
SIN-LTR
RRE
ZEOr
Utility
RNA Polymerase II promoter
Central Polypurine tract helps translocation
into the nucleus of non-dividing cells
Enhances the stability and translation of
transcripts
Marker to track shRNAmir expression
Mammalian selectable marker
Ampicillin bacterial selectable marker.
5' long terminal repeat
High copy replication and maintenance of
plasmid in E.coli
3' Self inactivating long terminal repeat
Rev response element
Bacterial selectable marker