Anatrace–Microlytic MCSG3 Formulations
Anatrace Products, LLC
Condition #
1
2
3
4
Well
A1
A2
A3
A4
5
6
7
8
A5
A6
A7
A8
9
10
11
12
Salt
Buffer
0.1 M Sodium Cacodylate: HCl, pH 6.5
0.1 M Na2HPO4: Citric Acid, pH 4.2
0.1 M Tris: HCl, pH 7
0.1 M Imidazole: HCl, pH 8
Precipitant
1 M Sodium Citrate Tribasic
2 M Ammonium Sulfate
1 M Sodium Potassium Tartrate
1 M Ammonium, Phosphate Dibasic
0.1 M MES: NaOH, pH 6
0.2 M Na2HPO4/KH2PO4, pH 6.2
0.1 M Imidazole: HCl, pH 8
0.1 M Imidazole: HCl, pH 8
1.26 M Ammonium Sulfate
2.5 M Sodium Chloride
10 % (w/v) PEG 8000
30 % (w/v) PEG 8000
A9
A10
A11
A12
0.1 M MES: NaOH, pH 6
0.1 M Bis-Tris: HCl, pH 5.5
0.1 M Bis-Tris: HCl, pH 6.5
0.1 M HEPES: NaOH, pH 7.5
1 M Potassium Sodium Tartrate
0.5 M Magnesium Formate
0.5 M Magnesium Formate
0.3 M Magnesium Formate
13
14
B1
B2
0.1 M Sodium Acetate: HCl, pH 4.5
2 M Ammonium Sulfate
1.8 M Ammonium Citrate Tribasic, pH 7.0
15
16
17
B3
B4
B5
0.1 M Tris: HCl, pH 8.5
0.1 M Citric Acid: NaOH, pH 3.5
0.1 M HEPES: NaOH, pH 7.5
0.5 % (w/v) PEG 5000 MME
25 % (w/v) PEG 3350
45 % (v/v) MPD
18
B6
0.2 M Lithium Sulfate
0.2 M Calcium Acetate Hydrate
0.2 M Sodium Chloride
0.8 M Potassium Sodium Tartrate
0.2 M Ammonium Acetate
0.05 M Bis-Tris: HCl, pH 6.5
45 % (v/v) PPG P400
0.1 M HEPES: NaOH, pH 7.5
12 % (w/v) PEG 3350
19
B7
0.005 M Cobalt Chloride, 0.005 M Magnesium Chloride,
0.005 M Cadmium Chloride, 0.005 M Nickel Chloride
20
21
B8
B9
0.2 M Sodium Chloride
0.2 M Calcium Acetate Hydrate
0.1 M Sodium Cacodylate: HCl, pH 6.5
0.1 M Imidazole: HCl, pH 8
2 M Ammonium Sulfate
20 % (w/v) PEG 1000
22
23
24
B10
B11
B12
0.2 M Magnesium Chloride
0.1 M Imidazole: HCl, pH 8
0.1 M Sodium Acetate: Acetic Acid, pH 4.5
0.1 M HEPES: NaOH, pH 7.5
15 % (v/v) Ethanol
20 % (v/v) 1,4-Butanediol
1.26 M Ammonium Sulfate
25
26
C1
C2
0.1 M Imidazole: HCl, pH 8
0.1 M Tris: HCl, pH 7
2.5 M Sodium Chloride
30 % (w/v) PEG 3000
27
28
C3
C4
29
C5
0.08 M Sodium Cacodylate: HCl, pH 6.5
25 % (v/v) 1,2-Propanediol, 10 % (v/v) glycerol
16 % (w/v) PEG 8000, 10 % (v/v) glycerol
30
31
C6
C7
0.1 M Bis-Tris Propane: HCl, pH 7
0.4 M Potassium Sodium Tartrate
1 M Ammonium Citrate Tribasic, pH 7.0
32
33
34
C8
C9
C10
0.2 M Ammonium Acetate
0.1 M Tris: HCl, pH 8.5
0.1 M Bis-Tris Propane: HCl, pH 7
0.1 M Bis-Tris: HCl, pH 6.5
3.2 M Sodium Chloride
2 M Sodium Formate
45 % (v/v) MPD
35
36
C11
C12
0.2 M Ammonium Acetate
0.05 M Zinc Acetate
0.1 M Tris: HCl, pH 8.5
45 % (v/v) MPD
20 % (w/v) PEG 3350
37
38
39
D1
D2
D3
0.2 M Magnesium Chloride
0.2 M Zinc Acetate
0.2 M Lithium Sulfate
0.1 M Tris: HCl, pH 7
0.1 M Sodium Cacodylate: HCl, pH 6.5
0.1 M Tris: HCl, pH 7
10 % (w/v) PEG 8000
10 % (v/v) Isopropanol
2 M Ammonium Sulfate
40
41
D4
D5
0.2 M Sodium Chloride
0.1 M Imidazole: HCl, pH 8
0.1 M Imidazole: HCl, pH 8
1 M Sodium Citrate
1 M Potassium Sodium Tartrate
42
43
D6
D7
0.2 M Zinc Acetate
0.2 M Lithium Sulfate
0.1 M MES: NaOH, pH 6
0.1 M CHES: NaOH, pH 9.5
10 % (w/v) PEG 8000
1 M Potassium Sodium Tartrate
44
45
46
D8
D9
D10
0.1 M Sodium Acetate: HCl, pH 4.6
47
48
49
50
D11
D12
E1
E2
8 % (w/v) PEG 4000
1.6 M Sodium Citrate
20 % (w/v) PEG 3350
0.8 M NaH2PO4, 0.8 M KH2PO4
51
52
E3
E4
53
54
55
0.2 M Sodium Chloride
0.1 M Sodium Cacodylate: HCl, pH 6.5
0.1 M Na2HPO4/KH2PO4, pH 6.2
0.16 M Magnesium Acetate
0.2 M Potassium Citrate Tribasic, pH 8.3
40 % (v/v) MPD, 5 % (w/v) PEG 8000
0.2 M Sodium Chloride
0.02 M Calcium Chloride
0.1 M HEPES: NaOH, pH 7.5
0.1 M Tris: HCl, pH 8.5
0.1 M NaH2PO4/K2HPO4, pH 6.2
0.1 M Sodium Acetate: HCl, pH 4.6
20 % (v/v) Ethanol
50 % (v/v) PEG 200
30 % (v/v) MPD
0.05 M Cesium Chloride
0.1 M Tris: HCl, pH 8
0.1 M MES: NaOH, pH 6.5
40 % (v/v) MPD
30 % (w/v) Jeffamine M-600
E5
E6
E7
0.1 M Sodium Citrate: HCl, pH 5
0.1 M Tris: HCl, pH 8
0.1 M Imidazole: HCl, pH 6.5
3.15 M Ammonium Sulfate
20 % (v/v) MPD
1 M Sodium Acetate
56
57
E8
E9
0.1 M HEPES: NaOH, pH 7.5
0.09 M HEPES: NaOH, pH 7.5
10 % (w/v) PEG 8000, 10 % (v/v) Ethylene Glycol
1.26 M Sodium Citrate, 10 % (v/v) Glycerol
58
59
E10
E11
0.1 M Bis-Tris Propane: HCl, pH 7
0.1 M Sodium Acetate: HCl, pH 4.6
2.5 M Ammonium Sulfate
0.8 M Lithium Sulfate
60
61
62
E12
F1
F2
0.1 M HEPES: NaOH, pH 7.5
0.1 M Citric Acid: NaOH, pH 3.5
0.1 M Tris: HCl, pH 8.5
1.4 M Sodium Citrate
2 M Ammonium Sulfate
0.3 M Magnesium Formate
63
64
F3
F4
0.2 M Magnesium Chloride
0.1 M Tris: HCl, pH 8.5
50 % (v/v) Ethylene Glycol
2.1 M DL-Malic Acid, pH 7.0
65
66
67
F5
F6
F7
1 M Ammonium Sulfate
0.1 M HEPES: NaOH, pH 7
0.5 % (w/v) PEG 8000
25 % (w/v) PEG 1500
30 % (v/v) Jeffamine M-600, pH 7.0
68
F8
0.1 M HEPES: NaOH, pH 7
5 % (v/v) Microlytic Mix(1), pH 7.0, 10 % (w/v) PEG 5000 MME
69
70
71
F9
F10
F11
0.2 M Magnesium Chloride
0.1 M Imidazole: HCl, pH 8
0.1 M HEPES: NaOH, pH 7.5
0.1 M Tris: HCl, pH 8.5
10 % (w/v) PEG 8000
15 % (v/v) Ethanol
20 % (w/v) PEG 1000
72
F12
0.2 M Sodium Chloride
0.1 M HEPES: NaOH, pH 7.5
30 % (v/v) PEG 400
73
74
75
76
77
G1
G2
G3
G4
G5
0.2 M Magnesium Chloride
0.1 M Imidazole: HCl, pH 8
0.1 M Na2HPO4: Citric Acid, pH 4.2
0.1 M Sodium Cacodylate: HCl, pH 6.5
35 % (v/v) MPD
40 % (v/v) Ethanol, 5 % (w/v) PEG 1000
50 % (v/v) PEG 200
10 % (w/v) PEG 1000, 10 % (w/v) PEG 8000
70 % (v/v) MPD
78
G6
0.17 M Ammonium Sulfate
79
80
81
G7
G8
G9
82
83
G10
G11
84
85
86
87
88
G12
H1
H2
H3
H4
89
90
91
92
93
H5
H6
H7
H8
H9
94
95
96
H10
H11
H12
0.1 M HEPES: NaOH, pH 7
0.2 M Magnesium Chloride
0.1 M HEPES: NaOH, pH 7.5
0.2 M Sodium Chloride
25.5 % (w/v) PEG 4000, 15 % (v/v) Glycerol
0.1 M BICINE: NaOH, pH 9
0.1 M BICINE: NaOH, pH 9
0.1 M Sodium Citrate: Citric Acid, pH 5.5
10 % (w/v) PEG 6000
10 % (v/v) MPD
40 % (v/v) 1,2-Propanediol
0.1 M Bis-Tris Propane: HCl, pH 7
1.5 M Ammonium Chloride
0.1 M Tris: HCl, pH 8.5
0.4 M Magnesium Formate
0.1 M Sodium Acetate: HCl, pH 4.6
0.1 M HEPES: NaOH, pH 7.5
0.1 M Sodium Acetate: HCl, pH 4.5
0.1 M HEPES: NaOH, pH 7.5
0.1 M Imidazole: HCl, pH 8
2 M Sodium Formate
10 % (w/v) PEG 3350
3 M Sodium Chloride
10 % (v/v) Isopropanol
20 % (w/v) PEG 3000
0.2 M Sodium Chloride
0.2 M Zinc Acetate
0.2 M Sodium Chloride
0.1 M Tris: HCl, pH 7
0.1 M Imidazole: HCl, pH 8
0.1 M HEPES: NaOH, pH 7.5
0.1 M CHES: NaOH, pH 9.5
0.1 M Tris: HCl, pH 8.5
1 M Sodium Citrate
20 % (v/v) 1,4-Butanediol
20 % (v/v) 1,4-Butanediol
30 % (v/v) PEG 400
1 M Ammonium, Phosphate Dibasic
0.2 M Lithium Sulfate
0.1 M Sodium Acetate: Acetic Acid, pH 4.5
0.1 M Bis-Tris Propane: HCl, pH 7
0.1 M Sodium Acetate: HCl, pH 4.6
2.5 M Sodium Chloride
0.7 M Sodium Citrate
0.2 M Proline
0.2 M Sodium Chloride
0.2 M Zinc Acetate
0.5 M Potassium Thiocyanate
Microlytic Mix is comprised of 1.8305 M Malonic Acid, 0.25 M Ammonium Citrate Tribasic, 0.12 M Succinic Acid, 0.3 M DL-Malic Acid, 0.4 M Sodium Acetate Trihydrate, 0.5 M Sodium Formate, 0.16 M Ammonium Tartrate Dibasic.
[McPherson, A. and Cudney, B. (2006) J. Struct. Biol. 156(3), 387-406.] Microlytic Mix at pH 7.0 is titrated with HCl. This mix is available from Anatrace, Product Nos. OPTIMIZE-131 and OPTIMIZE-132.
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