BIA Separations
CIM Convective Interaction Media®
PRODUCT SPECIFIC INFORMATION SHEET
Product: CIMmultus™ C4 HLD-800 Advanced Composite Column (Pores 2µm)
ABOUT CIMmultus™ C4 HLD-800 Advanced Composite Columns
Catalog number: 811.8136-2
CIMmultus™ C4 HLD-800 high-throughput multi-use Advanced Composite Column
consists of a unique polymeric monolith encased in a specially designed housing. It is
used for extremely fast, highly efficient separations of biomolecules like pDNA, viruses
and virus like particles in the hydrophobic interaction chromatography mode. The
following information is provided to ensure proper product care and maximal product
lifetime. Additional information about the product can be found in the Instruction
Manual 'CIMmultus™ 800 mL Advanced Composite Column'.
BASIC CHARACTERISTICS
Column chemistry:
Channel size:
Support matrix:
Tube dimensions:
Column end fitting:
Ligand density:
Dynamic binding capacity:
Operating flow rates:*
Recommended flow rate for
the scale up:
Maximum allowed pressure
over the column:
Operating temperature:
Chemical stability:
Recommended pH:
Storage temperature
solution:
Shelf life:
and
C4 HLD (Butyl, high ligand density)
Average pore radius: 950 nm – 1150 nm
Poly(butyl methacrylate -co- ethylene dimethacrylate)
Outer diameter: 105.0 mm; inner diameter: 65.0 mm; length: 150.0 mm; bed volume: 800 mL
Tri-Clamp® compatible sanitary end fitting with 25 mm flange size and 8 mm ID bore
1.1 – 1.3 mmol/mL wet monolith
≥ 2.5 mg pDNA/mL wet support
Conditions: pDNA (4.7 kbp) 0.2 mg/mL, 50 mM Tris-HCl, 10 mM EDTA buffer with 3M
ammonium sulfate, pH 7.2, flow rate 1600 mL/min
200 - 2000 mL/min; 30 - 305 cm/h; 0,25 - 2,5 CV/min
1100 mL/min; 168 cm/h; 1,37 CV/min
1.4 MPa; Please refer also to Instructional Manual, Section Connecting the CIMmultus
Advanced Composite Column.
4 °C (39°F) - 40 °C (104°F)
All commonly used aqueous buffers, 1 M NaOH, 0.1 M HCl, 3M ammonium sulfate, 8
M urea and 6 M guanidine hydrochloride. Avoid oxidizing agents. Avoid prolonged use
of concentrated acids (more than 0.5 M) like hydrochloric, sulphuric or acetic acid.
Working range 3 – 13
Cleaning in place 1 – 14
2 °C (36°F) to 25 °C (77°F), 10 mM NaOH; Please refer to the Instruction Manual for
appropriate storage procedure.
5 years
*Make sure the column is connected to the system according to the flow direction. Reversing the flow direction will destroy your column!
CHARACTERISTICS OF THE COLUMN HOUSING
The 800mL CIMmultus™ housing is made of carbon fiber reinforced epoxy thermoset material giving the column
structural strength of stainless steel. To avoid the risk of leachables, the surface of the housing is coated pinholefree with USP Class VI Parylene C. It can be used as a single-use disposable or as a multi-use column.
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Publication #: PSIS-M-C4HLD-800-2-1512
© 2015 by BIA Separations
ORDERING INFORMATION
Other CIMmultus™ 800 mL Products
Catalogue No.
811.5113-2
811.5114-2
811.6157-2
811.8140-2
Product description
CIMmultus™ QA-800 Advanced Composite Column (Quarternary amine) (Pores 2µm)
CIMmultus™ DEAE-800 Advanced Composite Column (Diethylamino) (Pores 2µm)
CIMmultus™ SO3-800 Advanced Composite Column (Sulfonyl) (Pores 2µm)
CIMmultus™ OH-800 Advanced Composite Column (Hydroxyl) (Pores 2µm)
Other CIMmultus™ C4 HLD Products
Catalogue No.
311.8136-2
411.8136-2
611.8136-2
1011.8136-2
Product description
CIMmultus™ C4 HLD-1 Advanced Composite Column (HLD Butyl) (Pores 2µm)
CIMmultus™ C4 HLD-8 Advanced Composite Column (HLD Butyl) (Pores 2µm)
CIMmultus™ C4 HLD-80 Advanced Composite Column (HLD Butyl) (Pores 2µm)
CIMmultus™ C4 HLD-8000 Advanced Composite Column (HLD Butyl) (Pores 2µm)
CIMmultus™ C4 HLD cGMP Compliant Products
Catalogue No.
911.8136-2
921.8136-2
931.8136-2
Adapters
Catalogue No.
422.0900
422.0901
422.0904
422.0905
Product description
CIMmultus™ C4 HLD-80 Advanced Composite Column cGMP compliant (HLD Butyl)
(Pores 2µm)
CIMmultus™ C4 HLD-800 Advanced Composite Column cGMP compliant (HLD Butyl)
(Pores 2µm)
CIMmultus™ C4 HLD-8000 Advanced Composite Column cGMP compliant (HLD Butyl)
(Pores 2µm)
Product description
Adapters PP, 10-32 female to 5/16-24 cone male (pack of 2)
Adapter PP, 5/16-24 flat female to 5/16-24 cone male (pack of 2)
Adapters SS, mini tc 25 mm to 10-32 cone female (pack of 2)
Adapters SS, tc 25 mm to 5/16-24 cone female (pack of 2)
For any additional information please contact us:
Tel.: +386 5 9699 500
[email protected]
www.biaseparations.com
Information and specifications contained here are, to the best of our knowledge, accurate and represented in good faith. They are intended to help you start
working with this new separation technology and are subject to change without notice. BIA Separations shall not be liable for errors contained herein or for
incidental or consequential damages in connection with the performance of use CIM. For more information on our products, visit our home page at:
http:://www.biaseparations.com or contact your local distributor. We reserve the right to alter the specification detail etc. without prior notice or liability.
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Publication #: PSIS-M-C4HLD-800-2-1512
© 2015 by BIA Separations