T
he culmination of apoptosis in vivo is phagocytosis of cellular corpses. During apoptosis, plasma membrane phospholipid asymmetry is lost, exposing phosphatidylserine (PS) externally.
Phagocytosis of apoptotic cells can be inhibited stereospecifically by PS and it’s structural
analogs, but not by other anionic phospholipids, suggesting that PS is specifically recognized.
A novel gene, cloned by phage display, appears to recognize PS on apoptotic cells. This gene, when
transfected into B and T lymphocytes, enables them to recognize and engulf apoptotic cells in a PSspecific manner. Flow cytometric analysis using antibodies to the PS receptor (clone designation MAb
217) suggests that the protein is expressed on the surface of macrophages, fibroblasts and epithelial
cells. The antibody, like PS liposomes, inhibited their phagocytosis of apoptotic cells and, in
macrophages, induced an anti-inflammatory state. The candidate PS receptor is highly homologous
to genes of unknown function in C. elegans and D. melanogaster, suggesting that the PS recognition
on apoptotic cells during their removal by phagocytes is highly conserved throughout phylogeny.
Fadok, VA et al.,(May 4, 2000) Nature 405:85-90, “A Receptor for
Phosphatidylserine-specific Clearance of Apoptotic Cells”.
Monoclonal
Anti-Human
Phosphatidylserine Receptor (clone 217)
L O T
S P E C I F I C A T I O N
Quality Control Analyses
Research Applications (protocols available)
Flow Cytometry: 100-200 µg/ml
Bioactive:
200 µg/ml; inhibits uptake of apoptotic
cells by macrophages expressing the
receptor
300 µg/ml; stimulates TGFβ secretion
Immunoblotting: 3 µg/ml
Apoptotic Cell Uptake Inhibition MAb 217 inhibits uptake of apoptotic
Jurkat T cells by several phagocytes. Mouse bone marrow-derived macrophages
were stimulated to induce the ability to recognize PS.
Product Description
Host / Ig Type:
mouse IgM
Purification:
protein G-chromatography
Immunogen:
human macrophages (HMDM) stimulated with TGFβ and β-glucan for 48 hours
Specificity:
binds to cells from multiple species; not `
human-specific
A
Tested Reactivity: human, mouse, chicken
Liquid Carrier:
phosphate-buffered saline
Storage:
-20˚C
Stability:
2 years
Unstimulated
HMDM
Glucan-stimulated
HMDM
Production Control Information
Lot Number:
Catalog Number:
Mass: 200 µg
C
012-0401
ABM-3099
B
Control
PI Liposomes
PS Liposomes
Control
PI Liposomes
PS Liposomes
Surface Receptor Recognition MAb 217 identifies a surface receptor expressed
at high levels on human macrophages which recognize PS in apoptotic cells.
Label Sample:
Anti-Human
Phosphatidylserine Receptor
Mouse
Monoclonal
IgM
Catalog #ABM-3099
200 µg
Lot 012-0401
Storage: -20˚C
A. binding of MAb 217 to unstimulated and stimulated (TGFβ/β-glucan) human
macrophages; histograms represent one of four separates analyses, using
macrophages from four different donors.
B. liposomes containing PS but not PI inhibit the binding of MAb 217 to unstimulated macrophages.
C. MAb 217 inhibits uptake of apoptotic cells by stimulated, but not by un-stimulated HMDM.
For Research Use only.
Pricing
200 µg / $295
Cascade BioScience
37 Pierrepont Road • Winchester, MA 01890 • 781-721-4758
www.cascadebioscience.com
Winchester, Massachusetts 01890
(781) 721-4758
www.cascadebioscience.com