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Sodium Chloride BioChemica
state of matter
Solid Solubility (20°C)
358 g/L (H2O) Melting point
801°C Boiling point
1461°C Formula
NaCl M
58.44 g/mol CAS-No.:
7647-14-5 HS-No.:
25010099 EC-No.:
231-598-3 Storage:
RT LGK:
10 - 13 Disposal:
14 WGK:
1 Specification
Assay (titr.)
min. 99.5 % pH (5 %; H2O; 20°C)
5.0 - 8.0 Heavy metals (as Pb)
max. 0.001 % Insoluble matter
passes test Bromide
max. 0.005 % Iodide
max. 0.001 % Phosphate
max. 0.001 % Sulfate
max. 0.01 % A (1 cm/1 M in water)
260 nm
max. 0.01 280 nm
max. 0.01 Literature
(1) Wallace, D.M. (1987) Methods Enzymol. 152, 41-48 Precipitation of nucleic acids.
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Sodium Chloride BioChemica
Comment
Sodium chloride is used in combination with ethanol to precipitate DNA. Should the DNA sample contain
sodium dodecyl sulfate (SDS), the detergent is most effectively removed by making the sample 0.2 M NaCl before
ethanol addition (usually by adding 0.04 volume 5 M NaCl followed by 2 volumes ethanol). SDS remains soluble
under this conditions (1). When removing ethidium bromide from DNA-containing solutions after CsCl density
centrifugation isopropanol (saturated with 5 M NaCl) is used. Two phases are formed during saturation. The upper
phase is the isopropanol phases. (If non-saturated isopropanol is used CsCl will precipitate while mixing the
probes!).
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