Journal of General Microbiology (1988), 134, 1391-1394.
1391
Printed in Great Britain
CORRIGENDUM
The Nucleotide Sequence of a Streptomycin 6-PhosphotransferaseGene from
a Streptomycin Producer
By H I D E N O R I S H I N K A W A , M A S A N O R I S U G I Y A M A
AND
OSAMU NIMI
Journal of General Microbiology (1987), 133, 1289-1296.
Throughout the paper, streptomycin 6-phosphotransferase (streptomycin 6-kinase) should read
streptomycin phosphotransferase (streptomycin kinase).
Corrected versions of Fig. 2, Table 1 and Fig. 3(a) are given overleaf.
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1392
H . S H I N K A W A , M. SUGIYAMA A N D 0. NIMI
GGTACCGGGGGCCAGCAGGGGGATGA
CCATGGCCCCCGGT'CGTCCCCCTACT
KpnI
CGTTTCCGGCGGCGAGCACCAGGTCGAAGCCGGGTTCCAGGCCGAGGTCGTCCAGCCGGGCCAGGTCGCCGAGGAGCCA
GCAAAGGCCGCCGCTCGTGGTCCAGCTTCGGCCCAAGGTCCGGCTCCAGCAGGTCGGCCCGGTCCAGCGGCTCCTCGGT
*27
GTCCTGTGCGGGCGCTTCGCGGCGGGCGACGGCGAGCATCGAGGAGTCGACGTCCACGCCGGTGCAGTGGTGGCCCCAG
CAGGACACGCCCGCGAAGCGCCGCCCGCTGCCGCTCGTAGCTCCT-CAGGTGCGGCCACGTCACCACCGGGGTC
*I 06
Safl
CTCGGCGAGGCGGATCGCGATCCGGCCGGTGCCGGTGCCGCACCCCGCGTCGAGCACTCGGGAGGCCGGGGTCAGCAACGCGCGC
GAGCCGCTCCGCCTAGCGCTAGGCCGGCCACGGCGTGGGGCGCAGCTCGTGAGCCCTCCGGCCCCAGTCGTTGCGCGCG
*I 85
AGAAGGAGGCCTCGCCGTGGACGTCGTGGCACCGACGCGGCGA~~~GTGCGAACCTCCGGGCGTATTCCTCCCCAGCCT
TCTTCCTCCGGAGCGGCACCTGCAGCACCGTGGCTGCGCCGCTCGGCACGCTTGGAGGCCCGCATAAGGA~GGGTCGGA
*264
h l e t Ser A s p His P r o G l y P r o G l y A l a V a l T h r P r o G l u L e u P i n e Gly V a l G l y G1y A s p
A T G A G T G A T C A C C C C GGG C C G GGG G C C G T C ACG C C G GAG C T G T T C G G C G T G G G C G G C G A C
T A C T C A C T A G T G G G G C C C G G C C C C C G G C A G T G C G G C C T C G A C AAG C C G C A C C C G C C G CTC*501
BclI
Sma I
T r p L e u A l a V a l T h r A l a G l y G l u S e r G l y A l a S e r V a l P h e A r g A l a A l a Asp A l a T h r
T G G C T G G C C G T C A C C G C G G G C GAA T C G G G C G C C T C C G T C TTT C G C G C C GCG G A C G C C A C C
P.CC G A C C G G C A G T G G C G C C C G C T T A G C C C G CGG AGG C A G AAA G C G CGG C G C C T G CGG T G G
"561
A r g T y r A l a L y s C y s V a l P r o A l a A l a A s p A l a A l a G l y L e u G l u A l a G l u A r g A s p Arq
CGG T A C G C C AAG T G C G T G C C C G C C G C G G A C G C G G C C G G T C T T GAG GCG GAA C G C G A C CGG
G C C A T G C G G T T C ACG C A C GGG CGG C G C C T G C G C C G G C C A GAA C T C C G C CTT G C G C T G G C C
*621
I l e A l a T r p L e u Ser G l y G l n G l y V a l P r o G l y P r o A r g V a l L e u A s p T r p T y r A l a G l y
A T C G C C T G G C T G A G C GGG CAG G G C G T A C C G G G C C C C C G C G T C C T C G A C T G G T A C G C C C-GT
T A G C G G A C C G A C T C G C C C G T C C C G C A T G G C C C G GGG G C G C A G GAG C T G A C C A T G CGG C C A
*681
A s p A l a G l y A l a C y s Leu V a l Thr A r g A l a V a l P r o G l y V a l P r o A l a A s p A r g V a l G l y
GAC GCG GGC G C C T G C C T G G T C A C C C G T G C C G T C C C C GGC G T A C C C G C T G A T CGG GTG G C C
C T G C G C C C G C G G A C G G A C CAG T G G G C A C G G C A G GGG C C G C A T GGG C G A C T A G C C C A C C C G
*741
A l a A s p A s p Leu A r g T h r A l a T r p G l y A l a V a l A l a A s p A l a V a l A r o A r r j L e u 111s G l u
G C C G A T G A C C T T C G C A C T G C C T G G GGG G C C G T C G C G G A C G C G G T C C G T CGG C T G C A C GAG
CGG C T A C T G GAA G C G T G A C G G A C C C C C C G G C A G C G C C T G C G C CAG G C A G C C G A C G T G C T C
*801
V a l P r o V a l A l a S e r C y s P r o P h e A r g A r g G l y L e u A s p Ser V a l V a l A s p A l a Ala A r g
G T G C C C G T G G C C T C G T G T C C G TTC C G C C G G GGG C T G G A C T C C G T G G T C G A C G C C G C C C G T
CGG CGG GCA
C A C GGG C A C CGG A G C A C A G G C AAG G C G G C C C C C G A C C T G AGG C A C
Safl
*861
-
A s p V a l V a l A l a A r g G l y A l a V a l His P r o G l u P h e L e u P r o V a l G l u G l n A r g L e u V a l
G A C G T G G T G G C C C G T G G C G C G G T G C A T C C G G A G T T C C T G C C G G T G GAG C A G C G G C T C G T T
C T G C A C C A C CGG G C A C C G C G C C A C G T A G G C C T C AAG G A C G G C C A C C T C G T C G C C G A G C A A
*921
Pro Pro A l a G l u Leu Leu A l a A r g L e u T h r G l y G l u L e u A l a A r g A r g A r g A s p G l n G l u
CCC C C G G C G GAG C T G C T G G C C C G G C T C A C C GGG GAG C T C G C C C G T CGG C G C G A T CAG GAG
G G G G G C C G C C T C G A C G A C CGG G C C GAG T G G C C C C T C GAG C G G G C A G C C G C G C T A G T C C T C
*981
Sac1
Fig. 2 (continued on facing page)
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Corrigendum
1393
A l a A l a A s p Thr V a l V a l C y s H i s G l y A s p L e u C y s L e u Pro A s n Ile V a l L e u H i s Pro
G C C G C C G A C ACG G T C G T C T G C C A C G G T G A T C T C T G C C T G C C C A A C A T C G T C C T C C A T C C G
CGG C G G C T G T G C CAG CAG ACG G T G C C A C T A GAG ACG G A C GGG T T G T A G CAG GAG G T A G G C
*I 0 4 1
G l u T h r L e u G l u V a l Ser G l y P h e I l e A s p L e u G l y A r g L e u G l y A l a A l a A s p A r g H i s
GAG A C C C T G GAG G T G T C G G G C T T C A T C G A C C T G GGA CGG C T C GGG GCG G C C G A C C G C C A C
C T C T G G G A C C T C C A C AGC C C G AAG T A G C T G G A C C C T G C C GAG C C C C G C CGG C T G G C G G T G
*I 101
A l a A s p L e u A l a Leu L e u L e u A l a A s n A l a A r g G l u T h r T r p V a l A s p G l u G l u A r g A l a
G C C G A C C T G G C G C T G C T G C T G G C C A A C GCG C G C GAG A C C T G G G T G G A C GAG GAG CGG GCG
CGG C T G G A C C G C G A C G A C G A C CGG T T G C G C GCG C T C T G G A C C C A C C T G C T C C T C G C C C G C
*1 1 6 1
A r g Phe A l a A s p A l a A l a Phe A l a G l u A r g T y r G l y I l e A l a P r o A s p P r o G l u A r q L e u
CGG T T C G C C G A C GCG GCG T T C G C C GAG C G T T A C G G C A T C G C C C C G G A C C C G GAA CGG C T G
G C C AAG C G G C T G C G C C G C AAG CGG C T C GCA ATG C C G T A G CGG G G C C T G G G C C T T G C C G A C
*I 221
A r g P h e T y r L e u H i s L e u A s p P r o L e u T h r T r p G l y ***
C G C T T C T A C C T C C A T C T C G A T C C G C T C A C C TGG G G C T A G GTCCTGTCGTCGAACTGCCGTCGTCGC
GCG AAG A T G GAG G T A GAG C T A G G C GAG T G G A C C C C G A T C CAGGACAGCAGCTTGACGGCAGCAGCC
*I 2 8 1
-
CCGAAGGGCGGCCCTGCGGGGTGAAGCCCCGCCTGCGCGGCGGCGCCTGGCAGGCACGCTCGCTTCG~~ACACGC~C~'~
GGCTTCCCGCCGGGACGCCCCACTTCGGGGCGGACGCGCCGCCGCGGACCGTCCGTGCGAGCGAAGC~TTGTG~~~~GAri~
P
*I 3 4 7
b
4
ACGGGCTCCCGAGGGTGCGGAGTCCGCTGTGTGTCGGACGCGTCCGGTCCGGCGGCGGGTGCGGGCGGGCCGTGGTGGTCC
TGCCCGAGGGCTCCCACGCCTCAGGCGACACAGCCTGCGCAGGCCAGGCCGCCGCCCACGCCC~CCCGGCACCACCA~G
*I 4 2 6
GTCGCGATGACCACCACCTGGTCCTCGGCGTACCAGACGCGCCGTTCGCCCTTCGGCGGGTTGAGCCTGATGCCCTGGrr
CAGCGCTACTGGTGGTGGACCAGGAGCCGCATGGTCTGCGCGGCAAGCGGGAAGCCGCCCAACTCGGACTACGGGACCA
*1505
CCCCGGTCCCGGTGCGGGTGATGTCGTGCCGGCGGTAGCCGATC
GGGGCCAGGGCCACGCCCACTACAGCACGGCCGCCATCGGCTAG
*I 584
Fig. 2. Nucleotide sequence of the 1.6 kb of S. griseus DNA containing the streptomycin kinase gene.
The amino acids predicted by the sequence are shown. Two inverted repeats which could potentially
form hairpin-loop structures as putative transcription-termination signals are indicated by underlining.
Table 1. Codon usage in the streptomycin kinase structural gene
TTT-Phe
TTC-Phe
TTA-Leu
TTG-Leu
CTT-Leu
CTC-Leu
CTA-Leu
CTG-Leu
ATT-Ile
ATC-Ile
ATA-Ile
ATG-Met
GTT-Val
GTC-Val
GTA-Val
GTG-Val
1
7
0
0
2
10
0
17
0
4
0
1
1
12
2
12
TCT-Ser
TCC-Ser
TCA-Ser
TCG-Ser
CCT-Pro
CCC-Pro
CCA-Pro
CCG-Pro
ACT-Thr
ACC-Thr
ACA-Thr
ACG-Thr
GCT-Ala
GCC-Ala
GCA-Ala
GCG-Ala
0
2
0
3
0
8
0
11
1
7
0
2
1
29
0
16
TAT-Tyr
TAC-Tyr
TAA-***
TAG-***
CAT-His
CAC-His
CAA-Gln
CAG-Gln
AAT-Asn
AAC-Asn
AAA-Lys
AAG-Lys
GAT-Asp
GAC-Asp
GAA-Glu
GAG-Glu
0
4
0
1
3
4
0
3
0
2
0
1
6
18
3
14
TGT-Cys
TGC-Cys
TGA-***
TGG-Trp
CGT-Arg
CGC-Arg
CGA-Arg
CGG-Arg
AGT-Ser
AGC-Ser
AGA-Arg
AGG-Arg
GGT-Gly
GGC-Gly
GGA-Gly
GGG-Gly
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1
4
0
6
6
9
0
12
1
1
0
0
3
14
1
7
1394
H . S H I N K A W A , M. SUGIYAMA A N D 0 . NIMI
SMK from S . griseus HUT 6037
50
.
. .
.
50
2d
-
, ,
I
I
1
I
I
100
I
.I
,
f
.1
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.
I
I .
I
:
150
I
'I
, . .,'
. . . .. . .. . . .
. . . ... _ ... .
L
'I I
..
,
,
I
,
I
,
I
8
200
4
-(.
*
*. . - . .
I
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3
,
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250
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._
I
# S
, _
m
*
I
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'
.
''
.
200-
250
-
Fig. 3 ( a ) Dot-matrix analysis of the amino acid sequence of the streptomycin kinase (SMK) from S.
griseus (272 amino acid residues) and the aminoglycoside3'-phosphotransferase(APH) from S .frudiue
(268 amino acid residues).
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