Atlas of Antinuclear Antibodies
■ Autoantibodies recognizing mitotic spindle apparatus related antigens
The main component protein of the microtubles of the spindles is tubulin, and the microtubules bind to motor proteins like kinesin
and dynein. Anti-spindle apparatus antibodies like NuMA antibodies possibly recognize the component proteins and the binding
proteins of the microtubules.
Anti-spindle apparatus antibody
Pattern
The spindle fibers between the poles are
stained in mitotic cells.
Antigen
Tubulin
Clinical
significance
The presence of anti-tubulin antibodies
were reported to occur in patients with
parasitic infections and those with GuillainBarre syndrome.
References
63, 64
photo 55
Anti-nuclear mitotic apparatus (NuMA)-1 antibody
Pattern
The spindle poles and the spindle fibers
around them are stained in mitotic cells.
Nuclear speckled staining can be seen in
interphase cells.
photo 56
Antigen
210kD protein
Other
analytical
methods
WB
Clinical
significance
These antibodies occur in patients with
Reference
65
autoimmune diseases like SS.
SLE: systemic lupus erythematosus, SSc: systemic sclerosis, MCTD: mixed connective tissue disease, SS: Sjögren's syndrome, PM/DM:
polymyositis/dermatomyositis, RA: rheumatoid arthritis, PBC: primary biliary cirrhosis, AIH: autoimmune hepatitis
32
Autoantibodies recognizing mitotic spindle apparatus related antigens
Copyright © 2016 MEDICAL & BIOLOGICAL LABORATORIES CO., LTD. All Rights Reserved.
Atlas of Antinuclear Antibodies
Anti-nuclear mitotic apparatus (NuMA)-2 antibody
Pattern
The spindle poles and the spindle fibers
are stained in mitotic cells. At anaphase/
telophase, the staining is hifted to the
intercellular bridge and the midbody. The
nucleus and cytoplasm show no staining in
the interphase cells.
photo 57
Antigen
kinesin-like protein HsEg5(130kD)
Other
analytical
methods
Immunoprecipitation
Clinical
significance
These antibodies occur in patients with
References
65, 66
Pattern
The poles of the mitotic spindle are stained
autoimmune diseases like SLE.
Anti-centriole antibody
in the mitotic cells and one or two dots are
stained in the cytoplasm of the interphase
cells.
Antigen
48kD heat shock proteins (enolase)
Clinical
significance
The antibodies occur infrequently in patients
References
67, 68
with Raynaud's syndrome and SSc.
photo 58
Autoantibodies recognizing mitotic spindle apparatus related antigens 33
Copyright © 2016 MEDICAL & BIOLOGICAL LABORATORIES CO., LTD. All Rights Reserved.