GE Healthcare
Instructions 56-0253-90 AI
Packing reservoirs RK 16/26, RK50
1. Introduction
3.2 Materials
The RK 16/26 (Code No. 18-8793-01) and RK 50 (Code No.
18-8790-01) packing reservoirs are designed for use with
the XK columns. Each unit consists of a cylinder with a
conical adaptor, which screws into the respective XK column,
and a screw cap, see exploded diagram below. The pressure
limit is 0.5 MPa (5 bar). The reservoir can also be used as an
eluent container.
The materials from which the individual parts of the packing
reservoir are made are listed in the spare parts list below.
All these materials may come in contact with the gel
suspension or eluent during the packing procedure.
2. Unpacking
The packing reservoir is delivered complete with flanged
capillary tubing with M6 connectors.
Please check the delivery against the packing list.
3. Description
3.1 Functional design
Please refer to the exploded diagram below for details.
Cylinder (4). The cylinder is a transparent plastic tube
threaded at each end and graduated at 20 ml intervals to
300 ml or 800 ml. Cylinder dimensions are as follows:
3.3 Chemical resistance
The packing reservoirs may be used with aqueous solutions
and nearly all organic solvents commonly used in liquid
chromatography of macromolecules, with the following
few exceptions: chlorinated hydrocarbons, acetone and
other ketones, aliphatic esters, phenol, ammonia, and
concentrations of alcohol greater than 20%. Solutions
containing more than 10% NaOH, 10% HCl or other strong
mineral acids, 5% acetic acid and trichloro-acetic acid,
should not be used.
The reservoir can be used at temperatures up to 50 °C and
at pressures up to 0.5 MPa (5 bar, 70 psi). It may be sterilized
with ethylene oxide. It should not be autoclaved.
4. Installation
1. Remove the adaptor from the XK column.
Length
Diameter (inner)
Volume
RK16/26
RK50
20 cm
5.9 cm
300 ml
40 cm
5.9 cm
800 ml
Conical adaptor (2). The adaptor screws onto the cylinder
and into the respective XK column. There are 3 conical
adaptors, one for each XK column series: XK16, XK26 and
XK50. The sealing against the columns is obtained via
the sealing ring (1). The volume of the conical adaptors is
50, 70 and 100 ml for the XK16, XK26 and XK50 columns,
respectively.
Cap (5). The screw cap screws onto either end of the
cylinders RK16/26 or RK50. The cap carries a M6 nipple with
capillary tubing (6) for connection to valves, connectors and
pump.
2. Select the appropriate conical adaptor (2) and screw it
onto the XK column. Check that the seal ring (1) and the
O-rings (3) are in place.
3. Screw the appropriate cylinder (4) onto the conical
adaptor.
4. Packing with the reservoir is described in the package
TM
insert included in every package of Sephacryl HR (High
Resolution) gels and in “Gel Filtration, Principles and
Methods”, a handbook available from GE Healthcare.
5. Cleaning
Suitable cleaning agents are soapy water or laboratory
detergents.
6. Spare parts
The exploded diagram shows the correct locations of
the different parts of the RK packing reservoirs. For
replacements, please order according to the spare part list
below using the appropriate code numbers.
Please note that the code numbers for both packing
reservoirs RK16/26 and RK50 are included in the same table.
Code No.
Detail No.
Designation
1
Seal ring
XK16
XK26
XK50
Conical adaptor
XK16
XK26
XK50
O-ring
Cylinder
Cap
Capillary tubing (flanged)
Domed nut, M6
2
3
4
5
6
7
RK16/26
RK50
Material
No. per pack
–
19-0190-01
–
–
–
18-8798-01
A
A
A
5
5
5
18-8787-01
18-8786-01
–
18-0253-91
18-8789-01
18-8792-01
18-8808-01*
18-2450-01
–
–
18-8785-01
18-0253-91
18-8788-01
18-8792-01
–
18-2450-01
B
B
B
A
C
B
D
E
1
1
1
5
1
1
1
4
Available but not illustrated
Flanging/Start-up Kit 220 V
120 V
FIanging Tip Kit 1.2 mm
Capillary tubing 1.2 mm i.d., 1.8 mm o.d.
Capillary tubing 0.8 mm i.d., 1.8 mm o.d.
*
19-5090-01
19-5079-01
18-4597-01
19-4370-01
19-7435-01
1
1
1
2m
2m
44 cm (0.8 mm i.d., 1.8 mm o.d.)
Material
A = (EPDM) Ethylenepropylenedione polymer
B = Reinforced acetal plastics
C = Acrylic plastic
D = TEFZEL
E = Polyethylene
Ordering information
Packing
reservoir
Code No.
Column
Code No.
Adaptor
Code No.
RK16/26
18-8793-01
XK16/20
XK16/40
XK16/70
XK16/100
XK26/20
XK26/40
XK26/70
XK26/100
XK50/20
XK50/30
XK50/60
XK50/100
18-8773-01
18-8774-01
18-8775-01
18-8776-01
18-1000-72
18-8768-01
18-8769-01
18-8770-01
18-1000-71
18-8751-01
18-8752-01
18-8753-01
AK16
18-8778-01
AK26
18-8766-01
AK50
18-8754-01
RK50
2
18-8790-01
Instructions 56-0253-90 Edition AI 03/2006
RK50
RK 16/26
7
7
6
6
5
5
3
3
4
4
3
3
2
2
1
1
Fig 1.
Instructions 56-0253-90 Edition AI 03/2006
3
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GE Healthcare representative for the most current information.
© 2006 General Electric Company – All rights reserved.
GE Healthcare Bio-Sciences AB, a General Electric company.
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56-0253-90 AI 04/2006
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