How to survive in the DJ lab? Good Laboratory Practice Mateu Pla-Roca September 2008 Safety Rules in the DJ lab NEVER WORK ALONE IN THE LABORATORY Personal Protective Equipment The personal protective equipment is there to give you added protection against any accidents that may occur while working in the lab. When you are in the lab you should always wear: • Lab Coat • Safety Glasses When you are working in the lab you should always wear: • Lab Coat • Safety Glasses • Gloves Gloves When necessary gloves are a crucial component in keeping the chemicals away from your skin. But be careful! Just because you have gloves on doesn’t mean you’re safe. • Choose gloves that provide the proper protection and limit the time you are in contact with the chemical. • It is always a good idea to frequently change out your gloves when working with hazardous chemicals. Blue gloves Green gloves Never pick up the phone with the gloves on. Never open the doors with gloves one Handling Chemicals Read the instructions carefully on bottles and containers before you begin to use the substance and seek advice if necessary Safety Hazard symbols • Check labels for symbols indicating flammability, reactivity, health concerns, and other potential hazards associated with the reagent. • Be aware of the date on reagents bottles, as some reagents will decompose and may pose dangers if used. Read the Material Safety Data Sheet (MSDS) FUME HOODS are an essential component in the lab. Use them to protect yourself from potential harmful fumes. When you are working with: • Toxic reagents • Volatile reagents Do not put your head inside the fume hood. Be aware that some toxic substances are odourless. A FUME HOOD is not a LAMINAR FLOW CABINET Proper disposal of Waste Never pour chemical waste down the sinks or into the garbage. Waste containers are available in the lab. LIQUID WASTE Dilute the solutions with water. Diluted acids and bases Alcohols acetone Toluene Check the pH before waste if you don’t know Good practice at the DJ lab Work areas should be kept clean and tidy at all times. Don’t leave sharps, glass..(razor blades, coverslips…) on the benches. Other people could be hurt!! Proper Labelling Proper labelling ensures that you know what chemical you or your colleagues are handling and prevents you from getting harmed by one that is not labelled correctly. Bottles, beakers, samples…must be identified with: • Substance • Date • Name H2SO4 0.1M 03-10-2006 Pierre Avoid Cross Contamination Never return unused chemicals to their original container Never pipette chemicals directly from the bottles. Fire Prevention Ways to prevent fires are: • Have good housekeeping practice. • Use the smallest amount of flammable solvents possible. • Keep the flammable solvents away from ignition sources. • Store flammables in a flammable storage cabinet. Clean your bench… That's one small step for a researcher, one giant leap for the DJ lab.
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