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Demonstration
of
By
H.
a Cytoplasmic
M.D.,
BRAUNSTEINER
AND
T
HE
MOST
by
the
of a thickness
The
method
film
this
ansd
method
isucleus
ansd
insg normal
of the
a large
ansd
pathologic
progress
ding
by technsical
it is
he observed
up
in the
to several
lonsg.
roundinsg
the
structure
was
cell
tsucleus
ansd
described
the
It
a similar
cells
has
and
has
of the
also
salivary
beets
glansd
located
Casperssons
above
by
ansd
as the
this
To
tionss
his
school’3
are
ansd nsearly
structure
on
proved
the
representations
clarify
plasma
cells
of the
hone
thick
and
to ansd surThis
Bernshard
et
al.’#{176}
(RXP)-containing
rich amounsts
cells
secretory
close
of RNP.
of
of the
of
pancreas,
cells of the stomach.’#{176}
folhicular
cels
of the thyroid,
activity
relations
identify
because
certain
RNP
containinsg
this problem
further,
the presenst
a result,
thinnsess
whorls.
and
exocritse
the
co-workers
of embed8
parallel
ribonsucleoprotein
other
cells containsing
ins the
However,
studies
concernsbe unsdertakens.6
.05 to .08
al.9
the
of a characteristic
canalicular
elements
ahoint
et
pepsin-producing
ins the
ansd
denise.
ins concentric
Daltons
By
2
hint
of suitable
other
laboratory
have
Bernihard
morphologic
theory,
on
of this
sectiotss
particulates
a “form-
nsew methods
They
to exist
over
visualized,
too
the discovery
Filamentous
or
by
ansd the
ins this
Therefore,
far
was
to the hasophilic,
Ins search
for
a certains
depensdenscy
beets demonsstrated.’2
synsthesis.
cell
w’as founsd
be
caused
Onsly slices
picture.
screens.’
microtomes.7’
obtains
to each
the
cells
could
by developinsg
to
insdependently
structure
cells
remained
substansce.
lie close
Both groups
related
them
substance
of the cytoplasm.
RNP,
made
is
microscope
ins convenstional
grounsd
They
culture
hematologic
thrombocytes
could
ansd tissues.
of these
studies
of liver
cells.
cytoplasm
microscopy
certain
ansd
been
laboratories
of cells
result
tissue
always
improvements
ins some
almost
atsy type
Ans unsexpected
structure
ins the
may
recenstly
I\’I.D.,
electrons
to electron
method,
leukocytes
has
possible
now
zone
of this
Cells
of the electron
beam.
a sufficiently
transsparent
of cultured
perinuclear
Decisive
ansd
directly
cytoplasm
modification
in
in spreading
them
Plasma
M.D.
power
can give
0.1
consisted
in
FELLINGER
PAKESCH
penetratinsg
transferring
parts
a slight
ith
used
K.
PROBLEM
limited
of approximately
first
war”
F.
DIFFICULT
rather
Structure
of this
of RXP
the
and
structure
glansd
proteins
described
We are nsot enstirely
convinced
cells do nsot show this structure.
paper
reports
a series
of observa-
marrow.
METHODS
Small
ansounsts
of
boise
marrow
fixed
for 24 hours
in a 2 per
of 7.5. Thereafter
the pieces
were
ferred
for dehydration
successively
From
the
Department
were
cent
withdrawn
solution
by
of
0504
sternal
in
rirssed
for 1 hour in flowing
insto 50 ier censt, 70 per
of Medicine,
School
of Medicine,
puncture
buffered
and
at
tal) water.
They
cemst, 96 per cent,
were
trans-
and,
twice,
University
of
Vienna,
Austria.
Submitted
January
26,
1953;
accepted
for publication
916
immediately
Thsyrode
February
16, 1953.
a
Vienna,
pH
“
From www.bloodjournal.org by guest on June 16, 2017. For personal use only.
BRAUNSTEINER,
100
per
the
pieces
cent
etisansol
were
5 C. for 20 hours
The
only
thse
-20
to
1 -TWo
consists
facilitate
spreadi
tsseshi
placed
wax’’
allow(’(l
‘Ihe
-20
ttitl
sectioned
nsicrographis
was
tensioni
C.
was
a Rockinig
of
Tlse
tlse
11g.
to
dry.
nsat
eriol
t akeni
renlaininig
24
ins bemszol
hsours.
They
a Iote
filaments
I hey
were
for
were
nted
nal
a
iii
Thse
very
fine
accordimig
were
to
floated
marrow
2 hours,
then
kept
oni
to
Phi lips
elect
The
at
(vt
non)
X
temperature
ins Or(ler
o ilasiss
of
screenis
solut
ion.
rensiove
the
elect
or
these
X
4(XK)
Iflicloscope
1951)
of 5($K)
of
per-
obtainsed
surface
nucleus.
cent
‘fontnwar’’
ins order
to
( nsso(Iel
ion
was
was
(lsange
a liquid
nig t he
a 0.1 nr
in Nylol
nsagnificat
the
ion.
sectioninsg
sectiomsinsg
on
sect
surnounIli
lift NI
nsaoli’
frona
lv insnsersed
onigi
for
knife
lie
exami
an
microtomsse.
block
in
t lieme
at
917
PAKESCII
After
C. for
advance
thse
tells
wts
53
nsetalhic
conicentt
h’ronis
each.
at
Canssbridge
frons
plasnssa
of
in
A smooth
sections
pant
AND
for 5 hsours.
coate(l
wit Ii a filnis
ott tilter jmpen.
gent
were
generally
1 hour
of 5 C.
IlonIssal
inn lange
finie copper
hey
were
The
at
extensions
C.
5
for
ins “esterwax”
t.sed
slight
C.
Inn.
cells
kel)t
at a temperature
liv
frons
to
and
nsicrotonse
fornsed
an(l
enibedded
FELLINGER
nnsicroscope.
lot)
X
10,0(s)
of
Theni
‘est en-
.
The
high
and
two
fit) kv.
0nusFItv’rIoNs
We
cases
examine(l
of
y-mveloma.
tsontssal
Tise
plasnsa
results
cells
were
and
plasnsa
funsdamenstallv
cells
of onse
the
same.
case
of
-
From www.bloodjournal.org by guest on June 16, 2017. For personal use only.
918
(YTc)PLAS.\IIC
Fin.
ture.
Fin.
knit
2.-Plasnna
In
the
cell
cvtoplasns
3.-ionic
filaments
to
of
a 3-ns
numerous
nsarrow
sections
vacuoles
can
eloma.
STRU(’TURE
IN
Large
nucleolus
nsitoclsonsdria
of
h)e seen.
can
All
a -rnyeloma.
4000
be
X.
PLASMA
CELLS
with
a densse,
observed.
S(XXJ
trannsitory
states
ccnsvoluted
struc-
X
fronss
the closely-
From www.bloodjournal.org by guest on June 16, 2017. For personal use only.
BRAUNSTEIXER,
FIG.
clusion
4.-Vacuolated
bodies
inn the
plasma
vacuoles.
FELLINGER
cell of
StMXJ X.
AND
a -nsyeloma.
919
PAKESCH
Mamsy
of
these
types
of cell show
in-
.
I
Fin.
crowding
The
5.-Plasnsa
the
cell
of
a y-zssveloma.
1(J,dXX)
X.
Note
conicentric
filansentous
structure
nucleus.
NsLcleus
The
were
showed
nstncleus
clearly
showed
sects,
a consvoluted
oftens
a distiiictlv
very
structure,
visible
lange
ins
as
pathologic
pnevioitsly
membranse.
Onse
cells.
observed
or
These
ins the
several
nucleoli
nsucleoli
nucleoli
genserally
of liver
From www.bloodjournal.org by guest on June 16, 2017. For personal use only.
920
CYTOPLASMIC
cells.’0
t4
to
censtral
the
may
be
The
signsiflcatsce
positions
STRUCTURE
of this
of
of conssiderahle
the
IX
structure
PLASMA
still remains
ins instracellular
nsucleolus
Fmc;. 6.-Normal
nsyeloblast
ins the cytoplasm.
The
of
tise
horse
cytoplasnn
nsarrov.
Large
is entirely
Fin.
plasns.
The
7.-Paransyeloblast
convoluted
Nunserous
structure,
as.j.
‘‘
of
tine
bone
Isorizomitally
mitochonsdria.
8(X)
nsucleolus.
honsogeneous.
.
with
obscure
proteins
but, accordinsg
metabolism,
it
importansce.
clsondria
L
CELLS
6000
Numerous
nsito-
X
1
.
nsarrow
sectionned.
of
ans
No
acute
filamentous
leukensia.
structure
Large
nucleolus
of
thse
cvto-
X.
Cytoplasm
The
cytoplasm
consisted
nnext to onse ansother
arounsd
almost
the
enstirely
isucleus.
of finse fllamenstous
Ins other
cells these
elemensts
filamensts
lvinig
were
From www.bloodjournal.org by guest on June 16, 2017. For personal use only.
BRAUNSTEINER,
partially
dislocated
ansci
the
empty
state,
tlse enstire cytoplasm
presenst
ins the vacuoles.
A conssiderable
FELLING
space
might
nsumber
ER
AND
formed
so
be vacuolized.
of mitoclsondnia
was
vacuolatecl.
Sometimes
oftens
were
921
PAKESCH
In a terminal
inclusion
observed
bodies
ins the
were
cvtoplasns.
I)ISCcS5I0N
Ins comparinsg
previously
tise
salivary
glanscl,
thyroid,
the
All
these
productions
Fin.
ous
S-Cell
pepsins
morphologic
are
ansdi
secretiots
of
liver
cells,
proclucinig
cells
cells
idenstity
rids
of the
structure
ins
and
a lvmplsosarconsa.
No
filanisentous
of
cells
of tise
follicular
ability
the
this
wills
cells
the
9-12
in common
Therefore,
c)f proteins.
cells
cells,
ansd
tise
have
ins plasma
exocrine
stomach,
obvious.
of
beconses
in RNP
cytoplasm
panscreatic
idenstity
structure
of differentiated
of structure
of
the
may
cytoplasm.
be
Nunsser-
mitochondria.
due
to
cells
the
filansenstoins
described
those
both
of these
without
ansy
same consditions.
Tise followinsg
types
of acute
sarcoma
of these
Deducinsg
()nslv
structure
2)
of cells
have,
therefore,
secreting
have
cases
from
the
cells
these
beets
as
structure
constenits
become
This
observed:
of an
two
anid
are
by
ions
cases
hroblast
tisey
specific
cases
myelo-
several
proeryt
although
rated
explamsat
ItNP
ins two
mi
structure,
demonsst
of
perfornsed
experimental
lymphocytes
myeloblasts
a similar
RXP-constainsinsg
under
was
paramyelobla.sts
cells
anscl
was
ol)servationis
isigh
cytoplasm
filamemstous
show
of RNP
ver
wlsens
ins tise
The
did
amounst
exansinsed
activity.
leukemia,
cells of a lympisosarroma,
the rat,
nson’mal
lymplsocytes
leinkemia,
amid fitsally
niormal
a comssiderable
1)
proteins
of
lymphatic
nonse
We
properties.
knsowns
of
s. In
cotstainsecl
stainsinsg.
remains
presenst,
does
tise
filamenstous
visible.
is a RNP
containing
apparatus
of thse
cytoplasm
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922
CYTOPLASMIC
for
The
the productions
latter
theory
STRUCTURE
of differentiated
is supported
by
IN
PLASMA
proteins
and
the authors.*
CELLS
for
its
extracellular
secretions.
SUMMARY
Its normal
and
torns structure
significance
pathologic
plasma
of the cytoplasm
of this structure
cells
of the
boise
has 1)eeis revealed
is briefly
discussed.
marrow,
by
a specific
electron
filamen-
microscopy.
The
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Sectioning
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*
Very
PORTER:
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Pallade
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has
112: 370,
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convincing
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The tubular
paper.’5’
16
the
form
presence
would
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1953 8: 916-922
Demonstration of a Cytoplasmic Structure in Plasma Cells
H. BRAUNSTEINER, K. FELLINGER and F. PAKESCH
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