PRODUCT INFORMATION
5-Lipoxygenase (potato)
Item No. 60400
Overview and Properties
Synonyms:
Source:
Potato Lipoxygenase
Fresh potato tubers
Batch specific information can be found on the Certificate of Analysis or by contacting Technical Support
Molecular Weight: 85 kDa • Monomer
Storage:
-80°C (as supplied)
Stability:
≥6 months
Purity:
batch specific (estimated by SDS-PAGE)
Supplied in:
batch specific
Protein
Concentration:
batch specific mg/ml
Activity:
batch specific U/ml
Unit Definition:
One unit of enzyme consumes one nanomole of oxygen per minute at 25°C in 0.1 M
phosphate buffer, pH 6.3, containing 200 μM linoleate and 5 mM EDTA (the activity
is about three times lower with arachidonate as substrate). 5-Lipoxygenase activity
is measured at 25°C by monitoring oxygen consumption using a Gilson Model 5/6 H
oxygraph equipped with a Clark oxygen electrode.
Laboratory Procedures
The enzyme preparation contains 2 M ammonium sulfate to precipitate and stabilize the enzyme for long
term storage. Upon dilution with 0.1 M phosphate buffer, pH 6.3, the precipitated enzyme goes back into
solution. If the excess ammonium sulfate is undesirable, it can be removed by standard desalting procedures.
The desalted and/or diluted enzyme is only stable for one week and gradually degrades to a low molecular
weight form accompanied by a decrease in specific activity. In the event that only a portion of the enzyme
is to be used in a single experiment, it is recommended that the enzyme be aliquoted into smaller sizes and
frozen at -80°C. 5-LO is relatively unstable at room temperature. Hence, to prevent loss of activity when
performing experiments, keep the stock vial of the enzyme on ice (0-4°C) at all times.
Description
5-LO catalyzes the first step in the biosynthesis of leukotrienes by oxidizing arachidonic acid to 5(S)-HpETE. The
enzyme from potato tubers is an 85 kDa protein. Like its mammalian counterparts, this enzyme exhibits both
lipoxygenase and LTA4 synthase activities.1 Potato 5-LO has an apparent Km of 33 μM and an apparent Vmax of
14 μmol/min/mg protein with arachidonic acid as substrate.2
References
1.Shimizu, T., Rådmark, O., and Samuelsson, B. Enzyme with dual lipoxygenase activities catalyzes
leukotriene A4 synthesis from arachidonic acid. Proc. Natl. Acad. Sci. USA 81, 689-693 (1984).
2. Reddanna, P., Whelan, J., Maddipati, K.R., et al. Purification of arachidonate 5-lipoxygenase from potato
tubers. Methods Enzymol. 187, 268-277 (1990).
WARNING
THIS PRODUCT IS FOR RESEARCH ONLY - NOT FOR HUMAN OR VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.
SAFETY DATA
This material should be considered hazardous until further information becomes available. Do not ingest, inhale, get in eyes, on skin, or on clothing. Wash thoroughly after
handling. Before use, the user must review the complete Safety Data Sheet, which has been sent via email to your institution.
WARRANTY AND LIMITATION OF REMEDY
Buyer agrees to purchase the material subject to Cayman’s Terms and Conditions. Complete Terms and Conditions including Warranty and Limitation of Liability information
can be found on our website.
Copyright Cayman Chemical Company, 04/05/2016
CAYMAN CHEMICAL
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